mRNA_H-elongata_contig62614.13835.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig62614.13835.1
Unique NamemRNA_H-elongata_contig62614.13835.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A835Z8U8_9STRA (Phosphatase 2C-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8U8_9STRA)

HSP 1 Score: 154 bits (389), Expect = 9.790e-43
Identity = 77/103 (74.76%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREGTMK 309
            NS + Q RIEAAGGFVS PPEPGLS+RVWLD+ MTQIGLAMARSIGDHAVK +GVIAEPEVT H + P D+F++LASDGVWEFISSQEAV+ I A+L +G M+
Sbjct:  328 NSPAEQQRIEAAGGFVSPPPEPGLSARVWLDREMTQIGLAMARSIGDHAVKGIGVIAEPEVTEHFLGPDDQFLILASDGVWEFISSQEAVETIAANLDKGCMR 430
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: D7FIB7_ECTSI (PPM-type phosphatase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FIB7_ECTSI)

HSP 1 Score: 147 bits (371), Expect = 6.810e-40
Identity = 84/157 (53.50%), Postives = 90/157 (57.32%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIV------------------------------------------------------PADKFVVLASDGVWEFISSQEAVDIINASLREGTMK 309
            NS   Q RIEAAGGFVS PPEPGLSSRVWLD AMTQIGLAMARSIGDHAVKS+GV+AEPEVTTH I                                                         DKF+VLASDGVWEF+ SQEA+DI+N SL EGTMK
Sbjct:  313 NSPKEQARIEAAGGFVSPPPEPGLSSRVWLDAAMTQIGLAMARSIGDHAVKSIGVVAEPEVTTHTIACMVSLHWLCTWVDRRQSQDRRKRCTLKAPRRFLEHLIENMGSSGSHKTKRTCVQAGDKFIVLASDGVWEFVKSQEAIDIVNTSLEEGTMK 469
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A7R9ZIN9_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZIN9_9STRA)

HSP 1 Score: 133 bits (334), Expect = 6.830e-38
Identity = 64/91 (70.33%), Postives = 76/91 (83.52%), Query Frame = 1 
Query:   37 GGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREGTMK 309
            GGFVS PPEPGLS+RVWLD   TQIGLAMARSIGDHAVK +GVIAEP VT HKI P D+F++LA+DGVWEFISS+EAV +++ ++  G  K
Sbjct:    2 GGFVSPPPEPGLSARVWLDSTCTQIGLAMARSIGDHAVKPIGVIAEPVVTQHKIHPDDEFMILATDGVWEFISSEEAVKVVSDNMHLGATK 92
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: B8CBL0_THAPS (PPM-type phosphatase domain-containing protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CBL0_THAPS)

HSP 1 Score: 134 bits (338), Expect = 1.380e-37
Identity = 66/93 (70.97%), Postives = 77/93 (82.80%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDII 279
            +S   Q RI  AGGFVS PPEPGLSSRVWLD + TQIGLAMARSIGDHAVK VGVIAEP VT H +   D+F++LA+DGVWEF+SS++AVDI+
Sbjct:  122 DSPGEQERIIQAGGFVSPPPEPGLSSRVWLDSSHTQIGLAMARSIGDHAVKGVGVIAEPVVTVHTVEKGDEFMILATDGVWEFLSSEDAVDIV 214
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A6U1PER2_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1PER2_9STRA)

HSP 1 Score: 137 bits (345), Expect = 5.670e-37
Identity = 69/100 (69.00%), Postives = 78/100 (78.00%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREG 300
            N  + Q RIE AGGFVS PPEPGLS+RVWLD  MTQIGLAM+RSIGDHAVK +GVI EPEV    I P D+F +LASDGVWEFI SQEAV+I+   L +G
Sbjct:  233 NLPTEQRRIEKAGGFVSPPPEPGLSARVWLDPEMTQIGLAMSRSIGDHAVKHIGVIPEPEVQIFDIAPEDQFFILASDGVWEFIDSQEAVNIVQQHLGQG 332
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: K0RB05_THAOC (PPM-type phosphatase domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RB05_THAOC)

HSP 1 Score: 139 bits (349), Expect = 1.780e-36
Identity = 68/100 (68.00%), Postives = 81/100 (81.00%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREG 300
            +S   + RI ++GGFVS PPEPGLSSRVWLD   TQ+GLAMARSIGDHAVK VGVIAEP V TH+IVP D+FV++A+DGVWEFI S  AV+I+   LR+G
Sbjct:  409 DSPGEKERILSSGGFVSPPPEPGLSSRVWLDPEQTQVGLAMARSIGDHAVKGVGVIAEPVVETHRIVPGDEFVIMATDGVWEFIDSDAAVEIVADRLRKG 508
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A6U3Z835_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A6U3Z835_9STRA)

HSP 1 Score: 125 bits (313), Expect = 3.540e-34
Identity = 64/98 (65.31%), Postives = 78/98 (79.59%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKI-VPADKFVVLASDGVWEFISSQEAVDIINASL 291
            +S   + RI  +GG+VS PPEPGL++RVWLD  MTQIGLAMARSIGDHAVK VGVIAEP V+ H I    D+FV++A+DGVWEFISS++AVDI+   L
Sbjct:   36 DSPGEKERILDSGGYVSPPPEPGLTARVWLDPNMTQIGLAMARSIGDHAVKGVGVIAEPVVSVHTIDEELDEFVIIATDGVWEFISSEDAVDIVGRCL 133
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A7S3L068_9STRA (Hypothetical protein n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3L068_9STRA)

HSP 1 Score: 131 bits (330), Expect = 7.280e-34
Identity = 64/99 (64.65%), Postives = 77/99 (77.78%), Query Frame = 1 
Query:    1 NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLRE 297
            +S   Q RIE+ GG+VS PP PGLSSRVWLD  +TQIGLAMARSIGDHA+  VGVIA+P VT   + P D F++LASDGVWEF+SSQ+AV+I+   L E
Sbjct:  389 DSPGEQARIESMGGYVSAPPAPGLSSRVWLDSDLTQIGLAMARSIGDHAIGEVGVIADPVVTERVLEPQDDFMILASDGVWEFLSSQDAVNIVGEHLEE 487
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A7S4EFC8_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4EFC8_9STRA)

HSP 1 Score: 130 bits (327), Expect = 1.420e-33
Identity = 66/100 (66.00%), Postives = 78/100 (78.00%), Query Frame = 1 
Query:   10 SSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREGTMK 309
            + Q RIE+ GG+V+  PEPGLSSRVWLDK  +QIGLAMARSIGDHAVKS+GVIAEP V+ H +   D F+VLA+DGVWEFISS EAV I+   L +G  K
Sbjct:  363 AEQERIESMGGYVTPAPEPGLSSRVWLDKECSQIGLAMARSIGDHAVKSIGVIAEPVVSFHDVRENDDFLVLATDGVWEFISSAEAVKIVAKHLHKGATK 462
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BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Match: A0A448ZK03_9STRA (PPM-type phosphatase domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZK03_9STRA)

HSP 1 Score: 130 bits (326), Expect = 2.140e-33
Identity = 63/100 (63.00%), Postives = 78/100 (78.00%), Query Frame = 1 
Query:   10 SSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAVKSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREGTMK 309
            + Q RIE  GG+V+  PEPGLSSRVWLDK  +QIGLAMARSIGDHAVKS+GVIAEP V+ H +   D+F++LA+DGVWEFISS E+V ++   L +G  K
Sbjct:  371 AEQERIERMGGYVTPAPEPGLSSRVWLDKECSQIGLAMARSIGDHAVKSIGVIAEPVVSFHNVCENDEFLILATDGVWEFISSSESVQLVAEHLHKGATK 470
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig62614.13835.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835Z8U8_9STRA9.790e-4374.76Phosphatase 2C-like domain-containing protein n=1 ... [more]
D7FIB7_ECTSI6.810e-4053.50PPM-type phosphatase domain-containing protein n=2... [more]
A0A7R9ZIN9_9STRA6.830e-3870.33Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
B8CBL0_THAPS1.380e-3770.97PPM-type phosphatase domain-containing protein (Fr... [more]
A0A6U1PER2_9STRA5.670e-3769.00Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
K0RB05_THAOC1.780e-3668.00PPM-type phosphatase domain-containing protein n=1... [more]
A0A6U3Z835_9STRA3.540e-3465.31Hypothetical protein n=1 Tax=Skeletonema marinoi T... [more]
A0A7S3L068_9STRA7.280e-3464.65Hypothetical protein n=1 Tax=Amphora coffeiformis ... [more]
A0A7S4EFC8_9STRA1.420e-3366.00Hypothetical protein n=1 Tax=Pseudo-nitzschia aust... [more]
A0A448ZK03_9STRA2.140e-3363.00PPM-type phosphatase domain-containing protein n=1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig62614contigH-elongata_contig62614:387..3239 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score145.6
Seed ortholog evalue1.3e-32
Seed eggNOG ortholog2880.D7FIB7
EggNOG free text desc.protein serine/threonine phosphatase activity
EggNOG OGsCOG0631@1,KOG0698@2759
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons2
Model size315
Cds size315
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931008.8792896-CDS-H-elongata_contig62614:386..5871622931008.8792896-CDS-H-elongata_contig62614:386..587Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig62614 387..587 +
1691679660.473495-CDS-H-elongata_contig62614:386..5871691679660.473495-CDS-H-elongata_contig62614:386..587Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig62614 387..587 +
1622931008.8970692-CDS-H-elongata_contig62614:3125..32391622931008.8970692-CDS-H-elongata_contig62614:3125..3239Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig62614 3126..3239 +
1691679660.485148-CDS-H-elongata_contig62614:3125..32391691679660.485148-CDS-H-elongata_contig62614:3125..3239Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig62614 3126..3239 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig62614.13835.1prot_H-elongata_contig62614.13835.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig62614 387..3239 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig62614.13835.1

>prot_H-elongata_contig62614.13835.1 ID=prot_H-elongata_contig62614.13835.1|Name=mRNA_H-elongata_contig62614.13835.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=105bp
NSFSSQVRIEAAGGFVSLPPEPGLSSRVWLDKAMTQIGLAMARSIGDHAV
KSVGVIAEPEVTTHKIVPADKFVVLASDGVWEFISSQEAVDIINASLREG
TMKVS
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mRNA from alignment at H-elongata_contig62614:387..3239+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig62614.13835.1 ID=mRNA_H-elongata_contig62614.13835.1|Name=mRNA_H-elongata_contig62614.13835.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2853bp|location=Sequence derived from alignment at H-elongata_contig62614:387..3239+ (Himanthalia elongata Himel1 dioecious)
AACTCCTTCTCTTCTCAGGTTCGTATTGAGGCCGCGGGAGGATTCGTCAG
CCTACCGCCCGAGCCAGGCCTCAGCTCCAGAGTGTGGCTAGACAAGGCCA
TGACTCAAATAGGACTAGCAATGGCCCGGTCGATCGGGGATCACGCGGTG
AAGTCCGTCGGGGTGATCGCCGAACCCGAGGTCACCACTCACAAGATAGT
GGTGAGCAAACAGCACGAACATGATGCTTGGTCGATTGATGACTATAAAA
GGATATGGACAGGTCGGCTGCGTTCGTTATACAGTAGGGTAAGGTCCACA
AGGCGGTTTAAGAAAGGGCGATATTTAGCGGCGGAAGGAGTCGTTCTACC
TACCGCGTATAGGCTATATGGCAACGTGGTTGGGGTCATCTTTGGCATCG
CCCGGTGTGATGACTCAGCACGTGTTTTGATGTGTTTTGATCACAAGTCG
AATAGTAACACGGAACAGACCGGTACATAAGGCAACCTAGGAAAAATCTC
ATTGGGTGTACTGAATAGTATATCAATGATAATGGAATATTTTATTCAAA
ACTATGTGCCTAATGGGGCGCTAAACAAAAATATGGATGACATTCCAGAT
ACGGAATGTTTTTTTGTGTGTATGCCATCAACTGCTTACACTACGCTTTT
GAGCTCAACAATCGGTGTCGATGAAGAGTTGCCCGCGCCTTCCGTCCACG
TTCTGGATGGAAATGACTTGTCGGGGATCAGACGGCAGTGTCTTCAGGGA
AAGCTTGGTAATCTGATCAAAGGTACTGTGTCGGAAGCTTTCTCCAATTC
CCTTCACCACCTGCCGGCGCAGAGTCAGCCGCCGAGTCCTTGAAAACTCG
CGAACTCACACCTGACCGACACCAGAGAATGAATGGGACAGGGTCTAGTC
GTGCCTCCACCCGCATTCGTTCGAATCGAAGGTTGGTGATCCCACCCGAC
GAGTTCATCCGCGTAGAAAACCCCCCTTGTTATCGAGGGGAAGTCACCGC
GTTGTTCCTCTGCTGCGGTGTGCCTAGCGCTGGTGCTCGTCAGGCGCCAA
TCTGCCCCCGTGCTGGAGACTGGCTTAAACCACCACCAGCCCTTGATCCA
CGCCGTGTCGTGCGAGAACTCAAGCACTGCCATACCCGACATGCTATTGA
GGGCGGTACATCCTTTTCACTCGAGACCGTGATCTCCGCGCGGACACCAC
TGTGCCGACAGGAGAAGTGTGCGATGCGTCTATTCTGGCTCACGACAAGG
GATCCTCTAATCGACGGTACGTTTCGCCTACCTTCAGGCGGCGGTGCGCC
TCAGACAACGGAGTGCCAACGTCGACGGCTCCGACGCCGCGACCTTCGAG
GCGCGTAAGAACGCTCGGTATGCTCGGTCGGGACGTGCCTCTTACGACTA
AGACGACTTACAACTATGCACTCTTGCGGTGAAACATTTTGAGCTCCTCG
GAAAGAGCGGATATGAGCTCTTAGCCCAGTTGGCCACACACACCGTGGGT
GGCACGGACGACGGGAAGGTAAATATCCTTCAATAGGATGGTAAAGGAGC
GCCTTCTGCGGGTGGTGTCCGTCGCGGGTTCGGGGCTTCTAACTTCCCCC
ATAACTTGGCAGGGAAAGAGGGGTTAGCGGTGTACGAGATGTGGGTAGAG
GTTCGTCGGGTATGAAGGGCGCCCGGTCGAGGGAATAATAGGGGTGGATT
CTGGAAAACTTATCTTTGATCTACCCCACTCGAAGTTGGCAAGTGTTGCC
GAGGAGCACACACTTGATTCGGTGTACTCCTTTTTTGGTCTAGTTTCGCT
ACGTACTGATATATCGCTGCTTCTTATCGCCCAATAACATTGGGGGGGGG
GGGATGCTTCACTATTTGTAGTCCACATGTCCCTAGTAATCTCTTCTTAT
AACGATTGCTTACATTTTGATTTCTCGATTATATCGTGCCCTCTCTGCTC
TATGGATTATCGAATGGAGCCTAATTTATTCAGAAGCGCCTAAAGCACTC
AACGCATAATATCTTGTTTGCACCAAAACATGGACGAAAATAGCCCATGT
CTGGAAAAGGGGTTATATTTTAGGCTTGCGTATACACATAGACATTATAT
ATATATATATATATATATATATATATATGTATCGTCTCCTCTAATAAATA
GTAAATAGTAAATAATATAGTACCACGTCCTAGTAAGCGCTGCTCGAGGT
CATGTTGTATGTGCCTAGGTTCTAGCTATTTTGATGAAGTGGTCGACGTC
GTATCTCCGACCGACCTCGTCTACTGCAAGGGATGTTAGAGCAAGTGAAG
ATATTCCTGGCGGCGTCTGTATGAGAATAAATGTTTTGTCAGCGGTCAAA
GCCCAAACAAACCGGATTGATTTTCCTTTTTTTAATTTATTTATGCAAGA
CCTGGTATTTTACCCCCGTCATACGCTCAACAGTCGAACAACAACCGACA
AACGCTCACGGGAAAGTTGCGCCATGAAAGAGGTAAAGGGGGAATAACAT
CAAAGGATCACCTCTCCAGCGGAAAGGGAACTTCTGTTGTTGTCTTTTAC
CCGTCTGCATTTTATTCATTAGTTGATGAATGCTTTTCCACTCGAGGCAA
CGGCACTGGTGGACGCAGGCGGGCAAGCTCGCTGCGTGTGCCTGGATATG
TTTTGTGTGTGGGCGTCGATCGTTTCCGAATAGCTGCCTCGTAATACCTC
ACCCCCTTTTATGACGTCGTGGTTTACGCGGATCAGCAGCCGGCGGACAA
GTTCGTGGTGCTGGCTTCGGACGGGGTCTGGGAGTTCATATCAAGCCAGG
AGGCCGTTGACATAATCAATGCCAGTCTGAGGGAGGGTACGATGAAGGTG
AGC
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Coding sequence (CDS) from alignment at H-elongata_contig62614:387..3239+

>mRNA_H-elongata_contig62614.13835.1 ID=mRNA_H-elongata_contig62614.13835.1|Name=mRNA_H-elongata_contig62614.13835.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=630bp|location=Sequence derived from alignment at H-elongata_contig62614:387..3239+ (Himanthalia elongata Himel1 dioecious)
AACTCCTTCTCTTCTCAGGTTCGTATTGAGGCCGCGGGAGGATTCGTCAG
CCTACCGCCCGAGCCAGGCCTCAGCTCCAGAGTGTGGCTAGACAAGGCCA
TGACTCAAATAGGACTAGCAATGGCCCGGTCGATCGGGGATCACGCGGTG
AAGTCCGTCGGGGTGATCGCCGAACCCGAGGTCACCACTCACAAGATAGT
GAACTCCTTCTCTTCTCAGGTTCGTATTGAGGCCGCGGGAGGATTCGTCA
GCCTACCGCCCGAGCCAGGCCTCAGCTCCAGAGTGTGGCTAGACAAGGCC
ATGACTCAAATAGGACTAGCAATGGCCCGGTCGATCGGGGATCACGCGGT
GAAGTCCGTCGGGGTGATCGCCGAACCCGAGGTCACCACTCACAAGATAG
TGCCGGCGGACAAGTTCGTGGTGCTGGCTTCGGACGGGGTCTGGGAGTTC
ATATCAAGCCAGGAGGCCGTTGACATAATCAATGCCAGTCTGAGGGAGGG
TACGATGAAGGTGAGCCCGGCGGACAAGTTCGTGGTGCTGGCTTCGGACG
GGGTCTGGGAGTTCATATCAAGCCAGGAGGCCGTTGACATAATCAATGCC
AGTCTGAGGGAGGGTACGATGAAGGTGAGC
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