mRNA_H-elongata_contig61388.13691.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig61388.13691.1
Unique NamemRNA_H-elongata_contig61388.13691.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: D7G5R8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5R8_ECTSI)

HSP 1 Score: 158 bits (400), Expect = 7.060e-45
Identity = 75/97 (77.32%), Postives = 89/97 (91.75%), Query Frame = 1 
Query:    1 QLGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            QLGGLFQKP+L+EKLLSKAPFRFLHD++ AV+ASTGF AGL+S +EL+SG++K+K  KI YLDK++ALVGICSG D+ VRPSKVVAGLEPEGTNALL
Sbjct:   12 QLGGLFQKPRLSEKLLSKAPFRFLHDVISAVTASTGFAAGLYSDEELDSGSVKDKGSKIGYLDKIIALVGICSGRDIDVRPSKVVAGLEPEGTNALL 108
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A835YT93_9STRA (Microtubule-binding protein MIP-T3-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YT93_9STRA)

HSP 1 Score: 135 bits (339), Expect = 1.020e-34
Identity = 65/97 (67.01%), Postives = 77/97 (79.38%), Query Frame = 1 
Query:    1 QLGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            +LG +  KPKLTEKLLSK PFRFLHD++ AV+A+TGF  GL+SGDEL+SG +KEK  K+AYLDK VA VG C G  V +R +KVVAGLEPE TNA L
Sbjct:   15 ELGAVISKPKLTEKLLSKPPFRFLHDVISAVTAATGFGTGLYSGDELDSGLVKEKGPKMAYLDKAVACVGFCQGTAVDIRSAKVVAGLEPENTNAFL 111
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A8J4D5E8_9CHLO (MIP-T3 domain-containing protein n=1 Tax=Volvox reticuliferus TaxID=1737510 RepID=A0A8J4D5E8_9CHLO)

HSP 1 Score: 116 bits (291), Expect = 1.780e-31
Identity = 56/93 (60.22%), Postives = 69/93 (74.19%), Query Frame = 1 
Query:   13 LFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            +F KPKL+EKLL K PFRFLHD+V AV +STGF  GLF GDEL+  AI+EK  K+AYL K++ +V +  G   P RP+K+VAGLEPE TN  L
Sbjct:   18 VFDKPKLSEKLLVKPPFRFLHDVVSAVQSSTGFAPGLFQGDELDGKAIQEKDAKVAYLRKIIDVVSMTLGEQCPARPNKIVAGLEPENTNVFL 110
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A7S3WCQ6_EMIHU (Hypothetical protein (Fragment) n=2 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3WCQ6_EMIHU)

HSP 1 Score: 118 bits (295), Expect = 8.270e-31
Identity = 61/96 (63.54%), Postives = 71/96 (73.96%), Query Frame = 1 
Query:    4 LGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            LG L ++PKLT  LLSK PFRFLHDIV  V+ +TGF  GLFS DEL+SG IKEK +KIAYL K+   V + SG  + VRP KVVAG+EPE TNA L
Sbjct:   27 LGPLIKRPKLTAALLSKPPFRFLHDIVSEVTRATGFADGLFSEDELQSGRIKEKELKIAYLSKIFKCVEVASGGALGVRPGKVVAGMEPENTNAFL 122
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A7S3YFX9_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3YFX9_HETAK)

HSP 1 Score: 114 bits (285), Expect = 8.880e-31
Identity = 56/102 (54.90%), Postives = 70/102 (68.63%), Query Frame = 1 
Query:    1 QLGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALLAVSNE 306
            +LGG+F KPKL+EKLLSK PFRF+HD +   +A TGF  GLF G EL+   +K+K  KIAYL+K +  +    G  + VR SK+VAGLEPE TN LLA   E
Sbjct:    1 KLGGIFPKPKLSEKLLSKPPFRFIHDCISNTTAETGFLEGLFEGAELDGKGMKDKGDKIAYLEKAITAICHAKGESIDVRASKIVAGLEPENTNLLLAALGE 102
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: R1FU15_EMIHU (MIP-T3 domain-containing protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=R1FU15_EMIHU)

HSP 1 Score: 118 bits (295), Expect = 2.650e-30
Identity = 61/96 (63.54%), Postives = 71/96 (73.96%), Query Frame = 1 
Query:    4 LGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            LG L ++PKLT  LLSK PFRFLHDIV  V+ +TGF  GLFS DEL+SG IKEK +KIAYL K+   V + SG  + VRP KVVAG+EPE TNA L
Sbjct:   14 LGPLIKRPKLTAALLSKPPFRFLHDIVSEVTRATGFADGLFSEDELQSGRIKEKELKIAYLSKIFKCVEVASGGALGVRPGKVVAGMEPENTNAFL 109
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A8J4AKX1_9CHLO (Uncharacterized protein n=2 Tax=Volvox TaxID=3066 RepID=A0A8J4AKX1_9CHLO)

HSP 1 Score: 118 bits (296), Expect = 3.960e-29
Identity = 58/93 (62.37%), Postives = 69/93 (74.19%), Query Frame = 1 
Query:   13 LFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            +F KPKL+EKLL K PFRFLHD+V AV ASTGF  GLF GDEL+  AI+EK  K+AYL K++ +V I  G   P RP+K+VAGLEPE TN  L
Sbjct:   18 VFDKPKLSEKLLVKPPFRFLHDVVSAVQASTGFAPGLFQGDELDGKAIQEKDAKVAYLRKIIDVVSITLGEQCPARPNKIVAGLEPENTNIFL 110
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A7S4NCB2_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Prymnesium polylepis TaxID=72548 RepID=A0A7S4NCB2_9EUKA)

HSP 1 Score: 110 bits (276), Expect = 9.190e-29
Identity = 53/96 (55.21%), Postives = 69/96 (71.88%), Query Frame = 1 
Query:    4 LGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            LG L ++PKL++ LL K PFRFLHDIV  V+ +TGF  GL+ GDEL+SG+IK+K  K+AYL K++  V I     + +R  KVVAG+EPE TNA L
Sbjct:   36 LGTLIKRPKLSDALLLKPPFRFLHDIVSEVTKATGFATGLYEGDELKSGSIKDKDTKVAYLSKIIKCVEIALNSQIAIRAGKVVAGMEPENTNAFL 131
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BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A7S3WJE7_9SPIT (Hypothetical protein n=3 Tax=Choreotrichia TaxID=141411 RepID=A0A7S3WJE7_9SPIT)

HSP 1 Score: 115 bits (287), Expect = 4.680e-28
Identity = 56/96 (58.33%), Postives = 70/96 (72.92%), Query Frame = 1 
Query:    4 LGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            LG + ++PKLT  LL K PFRFLHDIV  V+ +TGF  GLF  +EL+SG IKEK +KIAYL K++  V I +G  + +RP KVVAG+EPE TNA L
Sbjct:   49 LGAIIKRPKLTAALLGKPPFRFLHDIVSEVTRATGFAEGLFGEEELQSGKIKEKELKIAYLAKIIKSVEIATGKTITIRPGKVVAGMEPENTNAFL 144
[Close]
BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Match: A0A7J7QCI1_9CHLO (Microtubule-binding protein MIP-T3-domain-containing protein n=1 Tax=Scenedesmus sp. NREL 46B-D3 TaxID=2650976 RepID=A0A7J7QCI1_9CHLO)

HSP 1 Score: 115 bits (287), Expect = 6.780e-28
Identity = 56/96 (58.33%), Postives = 70/96 (72.92%), Query Frame = 1 
Query:    4 LGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESGAIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALL 291
            +  L  KPKLT+KLLSK PFRFLHD++ AV A TGF  GLFSG EL++ AI++K  K+AYL K+ A+V +  G  VP +P K+VAGLEPE TN  L
Sbjct:   18 IDPLVDKPKLTDKLLSKPPFRFLHDVISAVQAKTGFAPGLFSGAELDAHAIQDKEGKVAYLTKITAVVSMALGEPVPAKPLKIVAGLEPENTNLFL 113
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig61388.13691.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5R8_ECTSI7.060e-4577.32Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835YT93_9STRA1.020e-3467.01Microtubule-binding protein MIP-T3-domain-containi... [more]
A0A8J4D5E8_9CHLO1.780e-3160.22MIP-T3 domain-containing protein n=1 Tax=Volvox re... [more]
A0A7S3WCQ6_EMIHU8.270e-3163.54Hypothetical protein (Fragment) n=2 Tax=Emiliania ... [more]
A0A7S3YFX9_HETAK8.880e-3154.90Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
R1FU15_EMIHU2.650e-3063.54MIP-T3 domain-containing protein n=1 Tax=Emiliania... [more]
A0A8J4AKX1_9CHLO3.960e-2962.37Uncharacterized protein n=2 Tax=Volvox TaxID=3066 ... [more]
A0A7S4NCB2_9EUKA9.190e-2955.21Hypothetical protein (Fragment) n=1 Tax=Prymnesium... [more]
A0A7S3WJE7_9SPIT4.680e-2858.33Hypothetical protein n=3 Tax=Choreotrichia TaxID=1... [more]
A0A7J7QCI1_9CHLO6.780e-2858.33Microtubule-binding protein MIP-T3-domain-containi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig61388contigH-elongata_contig61388:2246..3057 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score158.3
Seed ortholog evalue1.9e-36
Seed eggNOG ortholog2880.D7G5R8
Preferred nameTRAF3IP1
KEGG koko:K19680
GOsGO:0000003,GO:0000122,GO:0001654,GO:0001655,GO:0001738,GO:0001817,GO:0001818,GO:0001822,GO:0001932,GO:0001933,GO:0002009,GO:0002119,GO:0002164,GO:0002682,GO:0002683,GO:0002697,GO:0002698,GO:0002831,GO:0002832,GO:0003002,GO:0003008,GO:0003674,GO:0005102,GO:0005215,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005813,GO:0005815,GO:0005829,GO:0005856,GO:0005929,GO:0005930,GO:0006355,GO:0006357,GO:0006810,GO:0006928,GO:0006935,GO:0006950,GO:0006970,GO:0006972,GO:0006996,GO:0007017,GO:0007018,GO:0007275,GO:0007389,GO:0007399,GO:0007423,GO:0007507,GO:0007610,GO:0007611,GO:0007612,GO:0008017,GO:0008092,GO:0008104,GO:0008150,GO:0008306,GO:0008589,GO:0009605,GO:0009628,GO:0009653,GO:0009790,GO:0009791,GO:0009792,GO:0009888,GO:0009889,GO:0009890,GO:0009892,GO:0009966,GO:0009968,GO:0009987,GO:0010468,GO:0010556,GO:0010558,GO:0010563,GO:0010605,GO:0010629,GO:0010646,GO:0010648,GO:0010970,GO:0015630,GO:0015631,GO:0016043,GO:0019219,GO:0019220,GO:0019222,GO:0021532,GO:0021915,GO:0022414,GO:0022607,GO:0022611,GO:0023051,GO:0023057,GO:0030030,GO:0030031,GO:0030326,GO:0030705,GO:0030990,GO:0030992,GO:0031076,GO:0031110,GO:0031113,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031333,GO:0031347,GO:0031348,GO:0031399,GO:0031400,GO:0031503,GO:0032101,GO:0032102,GO:0032268,GO:0032269,GO:0032271,GO:0032479,GO:0032480,GO:0032501,GO:0032502,GO:0032504,GO:0032648,GO:0032688,GO:0032838,GO:0032886,GO:0032991,GO:0033036,GO:0033043,GO:0035050,GO:0035107,GO:0035108,GO:0035113,GO:0035295,GO:0035735,GO:0035869,GO:0036064,GO:0036342,GO:0040011,GO:0040024,GO:0042073,GO:0042221,GO:0042325,GO:0042326,GO:0042330,GO:0042509,GO:0042532,GO:0042592,GO:0042733,GO:0042995,GO:0043009,GO:0043010,GO:0043053,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043254,GO:0043900,GO:0043901,GO:0044085,GO:0044087,GO:0044422,GO:0044424,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044463,GO:0044464,GO:0044782,GO:0045184,GO:0045879,GO:0045892,GO:0045934,GO:0045936,GO:0045992,GO:0045995,GO:0046425,GO:0046426,GO:0046907,GO:0048513,GO:0048519,GO:0048523,GO:0048568,GO:0048583,GO:0048585,GO:0048598,GO:0048609,GO:0048729,GO:0048731,GO:0048736,GO:0048793,GO:0048856,GO:0048878,GO:0050687,GO:0050688,GO:0050730,GO:0050732,GO:0050789,GO:0050793,GO:0050794,GO:0050877,GO:0050890,GO:0050896,GO:0051093,GO:0051098,GO:0051101,GO:0051128,GO:0051129,GO:0051171,GO:0051172,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051241,GO:0051246,GO:0051248,GO:0051252,GO:0051253,GO:0051493,GO:0051641,GO:0051649,GO:0051960,GO:0051961,GO:0055088,GO:0055115,GO:0060173,GO:0060255,GO:0060271,GO:0060429,GO:0065007,GO:0065008,GO:0065009,GO:0070507,GO:0070925,GO:0071840,GO:0072001,GO:0072359,GO:0080090,GO:0080134,GO:0097014,GO:0097542,GO:0097546,GO:0099111,GO:0099568,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1901620,GO:1901621,GO:1902531,GO:1902532,GO:1902679,GO:1902903,GO:1903506,GO:1903507,GO:1904892,GO:1904893,GO:1905515,GO:2000026,GO:2000112,GO:2000113,GO:2001141
EggNOG free text desc.intraciliary transport
EggNOG OGsKOG3809@1,KOG3809@2759
COG Functional cat.Z
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036
Hectar predicted targeting categoryother localisation
Exons2
Model size306
Cds size306
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930990.7474773-CDS-H-elongata_contig61388:2245..23731622930990.7474773-CDS-H-elongata_contig61388:2245..2373Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61388 2246..2373 +
1691679653.1893997-CDS-H-elongata_contig61388:2245..23731691679653.1893997-CDS-H-elongata_contig61388:2245..2373Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61388 2246..2373 +
1622930990.7621868-CDS-H-elongata_contig61388:2879..30571622930990.7621868-CDS-H-elongata_contig61388:2879..3057Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61388 2880..3057 +
1691679653.1991372-CDS-H-elongata_contig61388:2879..30571691679653.1991372-CDS-H-elongata_contig61388:2879..3057Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61388 2880..3057 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig61388.13691.1prot_H-elongata_contig61388.13691.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig61388 2246..3057 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig61388.13691.1

>prot_H-elongata_contig61388.13691.1 ID=prot_H-elongata_contig61388.13691.1|Name=mRNA_H-elongata_contig61388.13691.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=102bp
QLGGLFQKPKLTEKLLSKAPFRFLHDIVLAVSASTGFTAGLFSGDELESG
AIKEKAVKIAYLDKLVALVGICSGYDVPVRPSKVVAGLEPEGTNALLAVS
NE
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mRNA from alignment at H-elongata_contig61388:2246..3057+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig61388.13691.1 ID=mRNA_H-elongata_contig61388.13691.1|Name=mRNA_H-elongata_contig61388.13691.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=812bp|location=Sequence derived from alignment at H-elongata_contig61388:2246..3057+ (Himanthalia elongata Himel1 dioecious)
CAGCTGGGTGGGTTGTTCCAGAAGCCAAAACTGACAGAGAAACTTCTCTC
GAAGGCGCCATTCCGCTTTCTGCACGACATCGTCTTGGCAGTCTCCGCTT
CCACAGGCTTTACGGCTGGCTTGTTCAGGTGCATCTCCGACGATACGCTA
TGGTACGGTAAATAGTATATATATCTGGATCAATTAATGGGAAGTTGCGG
TCCGTGGAAATCGCCCCGCTTACTCCGGCGTTTGTGTGCGATTACTTCGG
GCTGCGCCGCAAAGACGGTATTACGACTCAATTATTGTAGAGCCGCAGAC
AACGATACACTGTACCTCGTCGCGGGCCGCCAGGCAAGTGACACTATTTC
GGTCAGGTTGTCTCGAATCGGCGGTAGAGCGGTAAAAATTCCCTCGGACG
CCGACGGTGCCCTTCCTTAGACGAAGCGAGCAAGCGCGCATTGACATCTA
CCTTCCTTCTTTACGCATCGACCTTGTGGTAGATTCAAAATCTAACGTCG
AACCTACCCACGATGAAAAGTCGAGCGTCGTATGGCGGTATCATCCCGTC
CCCTCGCGAATACATCTCGAGACTTGGGGCTTCAGCCATATTGCGTCTCT
CACCGCCCCGGCGCTACCTCACAAAATGCGATAGCGGCGATGAACTGGAA
TCTGGCGCTATCAAGGAGAAGGCGGTGAAGATCGCTTACCTAGACAAGCT
CGTGGCGCTCGTGGGGATATGTTCCGGATATGACGTCCCGGTTCGTCCAT
CCAAGGTCGTAGCGGGACTGGAGCCAGAGGGGACAAACGCACTGCTTGCG
GTAAGCAATGAG
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Coding sequence (CDS) from alignment at H-elongata_contig61388:2246..3057+

>mRNA_H-elongata_contig61388.13691.1 ID=mRNA_H-elongata_contig61388.13691.1|Name=mRNA_H-elongata_contig61388.13691.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=612bp|location=Sequence derived from alignment at H-elongata_contig61388:2246..3057+ (Himanthalia elongata Himel1 dioecious)
CAGCTGGGTGGGTTGTTCCAGAAGCCAAAACTGACAGAGAAACTTCTCTC
GAAGGCGCCATTCCGCTTTCTGCACGACATCGTCTTGGCAGTCTCCGCTT
CCACAGGCTTTACGGCTGGCTTGTTCAGCAGCTGGGTGGGTTGTTCCAGA
AGCCAAAACTGACAGAGAAACTTCTCTCGAAGGCGCCATTCCGCTTTCTG
CACGACATCGTCTTGGCAGTCTCCGCTTCCACAGGCTTTACGGCTGGCTT
GTTCAGCGGCGATGAACTGGAATCTGGCGCTATCAAGGAGAAGGCGGTGA
AGATCGCTTACCTAGACAAGCTCGTGGCGCTCGTGGGGATATGTTCCGGA
TATGACGTCCCGGTTCGTCCATCCAAGGTCGTAGCGGGACTGGAGCCAGA
GGGGACAAACGCACTGCTTGCGGTAAGCAATGAGCGGCGATGAACTGGAA
TCTGGCGCTATCAAGGAGAAGGCGGTGAAGATCGCTTACCTAGACAAGCT
CGTGGCGCTCGTGGGGATATGTTCCGGATATGACGTCCCGGTTCGTCCAT
CCAAGGTCGTAGCGGGACTGGAGCCAGAGGGGACAAACGCACTGCTTGCG
GTAAGCAATGAG
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