mRNA_H-elongata_contig58834.13384.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig58834.13384.1
Unique NamemRNA_H-elongata_contig58834.13384.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: D8LST5_ECTSI (DNA-binding protein HU n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LST5_ECTSI)

HSP 1 Score: 115 bits (287), Expect = 3.270e-27
Identity = 57/87 (65.52%), Postives = 70/87 (80.46%), Query Frame = 1 
Query:    1 VRKPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKE 261
            +RKP VV  + ER+ L+K QAEAA+ AFT+ ++  V+DGNKI+LQGFGTFE R R GR GRNPRTGE ++IKATT PAFSASK FK+
Sbjct:   67 LRKPAVVEAIAERSNLDKNQAEAALAAFTDVVMQEVADGNKISLQGFGTFEGRARKGRTGRNPRTGEELKIKATTAPAFSASKAFKD 153
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: A0A835Z9S8_9STRA (DNA-binding protein HU n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9S8_9STRA)

HSP 1 Score: 102 bits (254), Expect = 2.700e-23
Identity = 48/91 (52.75%), Postives = 71/91 (78.02%), Query Frame = 1 
Query:    1 VRKPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQERE 273
            ++K   VA +  ++GL+KTQ +AA+ AF +T+++ V+DG K++L GFGTFESR+RA RQGRNP+TGE + I A+  PAFSA+K FK++ +E
Sbjct:    1 MKKADFVASISAKSGLSKTQTDAAISAFMDTVMEAVADGKKVSLVGFGTFESRERAARQGRNPQTGEALSIPASKAPAFSAAKAFKDRVKE 91
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: A0A6L5GQ70_9FIRM (HU family DNA-binding protein n=3 Tax=Eubacteriaceae TaxID=186806 RepID=A0A6L5GQ70_9FIRM)

HSP 1 Score: 97.8 bits (242), Expect = 1.310e-21
Identity = 48/85 (56.47%), Postives = 65/85 (76.47%), Query Frame = 1 
Query:    7 KPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKE 261
            K Q+VA + E++GL+K ++E A+ AFTE+IV+ +  G+K++L GFGTF+ R RA RQG NPRT EPIEIKA+  PAF A K FK+
Sbjct:    3 KTQLVASISEKSGLSKKESEKALSAFTESIVEALQKGDKVSLVGFGTFDVRHRAARQGLNPRTKEPIEIKASNAPAFKAGKAFKD 87
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: E6MGM2_9FIRM (DNA-binding protein HU n=1 Tax=Pseudoramibacter alactolyticus ATCC 23263 TaxID=887929 RepID=E6MGM2_9FIRM)

HSP 1 Score: 97.4 bits (241), Expect = 1.870e-21
Identity = 49/85 (57.65%), Postives = 62/85 (72.94%), Query Frame = 1 
Query:    7 KPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKE 261
            K ++VA M E+ GL K  AE A+ AFT+TIV+ +  G+K++L GFGTF+ R RA RQG NPRT EPIEIKA+  PAF A K FK+
Sbjct:    3 KSELVAAMAEKTGLPKKDAEKALTAFTDTIVETLQKGDKVSLVGFGTFDVRHRAARQGLNPRTKEPIEIKASDAPAFKAGKAFKD 87
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: UPI001A0860CC (HU family DNA-binding protein n=1 Tax=Synechococcales cyanobacterium T60_A2020_003 TaxID=2774349 RepID=UPI001A0860CC)

HSP 1 Score: 96.3 bits (238), Expect = 7.230e-21
Identity = 51/103 (49.51%), Postives = 66/103 (64.08%), Query Frame = 1 
Query:   13 QVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQERENQAHRLSIAPPPPSDT 321
            ++V  + ++  + K +A+A V A  +TI+D VS GNK+ L GFGTFE+R R  R+GRNP+TGE I I ATTVPAFSA K FKE           +APP P  T
Sbjct:    5 ELVDAIAQKVSVTKKEADAIVGAMVDTIMDTVSSGNKVTLVGFGTFEARNRQAREGRNPKTGEAIAIPATTVPAFSAGKQFKEM----------VAPPAPKAT 97
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: A0A7S2W3F4_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2W3F4_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 9.950e-21
Identity = 47/88 (53.41%), Postives = 67/88 (76.14%), Query Frame = 1 
Query:    1 VRKPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQ 264
            VRKPQ+VA++ E+ G+ K  ++AA+ A  E+IV+ VS GNK+++ GFGTF+   RA R+GRNP+TGE I+IKA+  PAF+  K FKE+
Sbjct:   49 VRKPQLVAKIAEKTGMTKADSDAALAAVLESIVEEVSAGNKVSMIGFGTFKLTSRAARKGRNPKTGETIDIKASNRPAFTVGKAFKER 136
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: A0A4R3Z7K3_9FIRM (DNA-binding protein HU-beta n=3 Tax=Erysipelotrichaceae TaxID=128827 RepID=A0A4R3Z7K3_9FIRM)

HSP 1 Score: 95.1 bits (235), Expect = 1.380e-20
Identity = 49/84 (58.33%), Postives = 60/84 (71.43%), Query Frame = 1 
Query:    7 KPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFK 258
            K ++V ++ E AGL KTQAEAA+ AF ETI D +  G+KI L+GFGTFE RQR  R+GRNPRTGE + I A+  PAF AS   K
Sbjct:    3 KTELVEKVAESAGLTKTQAEAAINAFVETITDGLKAGDKITLKGFGTFEVRQREAREGRNPRTGETMTIAASKAPAFKASSALK 86
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: K9U4A1_CHRTP (Bacterial nucleoid protein Hbs n=3 Tax=Chroococcidiopsis TaxID=54298 RepID=K9U4A1_CHRTP)

HSP 1 Score: 95.1 bits (235), Expect = 1.460e-20
Identity = 47/86 (54.65%), Postives = 64/86 (74.42%), Query Frame = 1 
Query:    7 KPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQ 264
            K ++V  + E+A + K QA+A + A  ETI+D VS G+K+ L GFG+FESR+R  R+GRNP+TG+ +EI AT VPAFSA K FKE+
Sbjct:    3 KGELVDSIAEKASVTKKQADAVLTAALETIIDAVSTGDKVTLVGFGSFESRERKAREGRNPKTGDKMEIPATKVPAFSAGKLFKEK 88
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: UPI000349D0E7 (HU family DNA-binding protein n=1 Tax=Pseudanabaena sp. PCC 6802 TaxID=118173 RepID=UPI000349D0E7)

HSP 1 Score: 95.1 bits (235), Expect = 1.460e-20
Identity = 46/86 (53.49%), Postives = 64/86 (74.42%), Query Frame = 1 
Query:    7 KPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQ 264
            K ++V  + ++A +NK QA+  + A  E IVD VS GNK+ L GFG+FE R+R  R+GRNP+TG+ +EI ATTVPAFSA K+F+E+
Sbjct:    3 KGELVDAIAKKASVNKKQADEVLTATLEVIVDAVSSGNKVTLVGFGSFEPRERKAREGRNPKTGDKMEIPATTVPAFSAGKSFRER 88
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BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Match: A0A7S1Y344_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1Y344_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 1.530e-20
Identity = 46/88 (52.27%), Postives = 66/88 (75.00%), Query Frame = 1 
Query:    1 VRKPQVVAEMMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQGRNPRTGEPIEIKATTVPAFSASKNFKEQ 264
            VRKP +V  + ER G +K+++  A+ A  +TI++ V+DG K+NLQGFGTF+  +R  R GRNP+TGE +EIKA+  P+FSA+K FKE+
Sbjct:   66 VRKPALVEMVAERMGTSKSESTQAIEAVVDTIMEVVADGKKLNLQGFGTFKLSERKARTGRNPKTGESLEIKASKTPSFSAAKGFKEK 153
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig58834.13384.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LST5_ECTSI3.270e-2765.52DNA-binding protein HU n=2 Tax=Ectocarpus TaxID=28... [more]
A0A835Z9S8_9STRA2.700e-2352.75DNA-binding protein HU n=1 Tax=Tribonema minus Tax... [more]
A0A6L5GQ70_9FIRM1.310e-2156.47HU family DNA-binding protein n=3 Tax=Eubacteriace... [more]
E6MGM2_9FIRM1.870e-2157.65DNA-binding protein HU n=1 Tax=Pseudoramibacter al... [more]
UPI001A0860CC7.230e-2149.51HU family DNA-binding protein n=1 Tax=Synechococca... [more]
A0A7S2W3F4_9STRA9.950e-2153.41Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]
A0A4R3Z7K3_9FIRM1.380e-2058.33DNA-binding protein HU-beta n=3 Tax=Erysipelotrich... [more]
K9U4A1_CHRTP1.460e-2054.65Bacterial nucleoid protein Hbs n=3 Tax=Chroococcid... [more]
UPI000349D0E71.460e-2053.49HU family DNA-binding protein n=1 Tax=Pseudanabaen... [more]
A0A7S1Y344_9STRA1.530e-2052.27Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig58834contigH-elongata_contig58834:197..2050 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score96.3
Seed ortholog evalue1.3e-17
Seed eggNOG ortholog1128427.KB904821_gene2844
Preferred namehup
KEGG koko:K03530
GOsGO:0000976,GO:0001067,GO:0001130,GO:0001216,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003690,GO:0003700,GO:0005488,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005886,GO:0006139,GO:0006259,GO:0006355,GO:0006725,GO:0006807,GO:0006873,GO:0006875,GO:0006879,GO:0006950,GO:0007154,GO:0008150,GO:0008152,GO:0009267,GO:0009605,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0009991,GO:0010106,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0016020,GO:0016043,GO:0019219,GO:0019222,GO:0019725,GO:0030003,GO:0030312,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031667,GO:0031668,GO:0031669,GO:0032991,GO:0032993,GO:0033554,GO:0034641,GO:0040007,GO:0042262,GO:0042592,GO:0042594,GO:0043170,GO:0043565,GO:0044212,GO:0044237,GO:0044238,GO:0044260,GO:0044424,GO:0044444,GO:0044464,GO:0045893,GO:0045935,GO:0046483,GO:0046916,GO:0048518,GO:0048522,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050896,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0051716,GO:0055065,GO:0055072,GO:0055076,GO:0055080,GO:0055082,GO:0060255,GO:0065007,GO:0065008,GO:0071496,GO:0071704,GO:0071840,GO:0071944,GO:0080090,GO:0090143,GO:0090304,GO:0097100,GO:0097159,GO:0098771,GO:0140110,GO:1901360,GO:1901363,GO:1902680,GO:1903506,GO:1903508,GO:1990837,GO:2000112,GO:2001141
EggNOG free text desc.Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions
EggNOG OGs1G6UT@1117,1HBG8@1150,COG0776@1,COG0776@2
COG Functional cat.L
Best tax levelOscillatoriales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03032,ko03036,ko03400
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionHistone-like bacterial DNA-binding protein
Ec32 orthologEc-15_002670.1
Exons3
Model size1139
Cds size447
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig58834.13384.1prot_H-elongata_contig58834.13384.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig58834 862..2023 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930954.748279-UTR-H-elongata_contig58834:196..8611622930954.748279-UTR-H-elongata_contig58834:196..861Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig58834 197..861 -
1691679639.1209936-UTR-H-elongata_contig58834:196..8611691679639.1209936-UTR-H-elongata_contig58834:196..861Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig58834 197..861 -
1622930954.8231707-UTR-H-elongata_contig58834:2023..20501622930954.8231707-UTR-H-elongata_contig58834:2023..2050Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig58834 2024..2050 -
1691679639.1759756-UTR-H-elongata_contig58834:2023..20501691679639.1759756-UTR-H-elongata_contig58834:2023..2050Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig58834 2024..2050 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930954.7685235-CDS-H-elongata_contig58834:861..10671622930954.7685235-CDS-H-elongata_contig58834:861..1067Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 862..1067 -
1691679639.1339872-CDS-H-elongata_contig58834:861..10671691679639.1339872-CDS-H-elongata_contig58834:861..1067Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 862..1067 -
1622930954.7859845-CDS-H-elongata_contig58834:1466..16221622930954.7859845-CDS-H-elongata_contig58834:1466..1622Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 1467..1622 -
1691679639.144454-CDS-H-elongata_contig58834:1466..16221691679639.144454-CDS-H-elongata_contig58834:1466..1622Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 1467..1622 -
1622930954.8062859-CDS-H-elongata_contig58834:1938..20231622930954.8062859-CDS-H-elongata_contig58834:1938..2023Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 1939..2023 -
1691679639.1634083-CDS-H-elongata_contig58834:1938..20231691679639.1634083-CDS-H-elongata_contig58834:1938..2023Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig58834 1939..2023 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig58834.13384.1

>prot_H-elongata_contig58834.13384.1 ID=prot_H-elongata_contig58834.13384.1|Name=mRNA_H-elongata_contig58834.13384.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=149bp
MMERAGLNKTQAEAAVVAFTETIVDFVSDGNKINLQGFGTFESRQRAGRQ
GRNPRTGEPIEIKATTVPAFSASKNFKEQERENQAHRLSIAPPPPSDTPR
VRHPIVSLTRRILYISGMLAGSNEKRPLLPSEAQRFRTRWIYRYRVYV*
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mRNA from alignment at H-elongata_contig58834:197..2050-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig58834.13384.1 ID=mRNA_H-elongata_contig58834.13384.1|Name=mRNA_H-elongata_contig58834.13384.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1854bp|location=Sequence derived from alignment at H-elongata_contig58834:197..2050- (Himanthalia elongata Himel1 dioecious)
GTGAGAAAGCCGCAGGTTGTTGCGGAGATGATGGAGAGAGCCGGCCTCAA
CAAAACGCAGGCGGAAGCAGCCGTGGTCGCGTTCACGGAGACTATCGTCG
ATTTCGTGTCTGGTAGGTTTAGCGCCCGCCCGCCACCGGTGCGCCCCCCA
AAACATGTATATCTAGTTCAGGGACCGGAACCGATACCCAAAAAAATCGG
CGTCCACCTCGCATGGTCACTGCGGTACTTGATGGTACCCCAGTCATCTG
ACGCAATTGTGAGAAACAGAGGGAAAATGTCGGTAGAAGAGTCATTGAGG
AGACGCCAGCAGCAGGCGGCGGGCAATGGCGCCCTCATTCCGCGGTTGAG
TTGCACGGTGTACGGTATAGTGACCGGGCGTGATTTTGTATGTCCGTGTT
GTGTGCTTTCTGTTTGTTGATGCTTCAGATGGGAATAAGATCAACTTGCA
GGGGTTCGGCACGTTTGAGTCTCGCCAGAGGGCGGGTCGGCAGGGCCGCA
ACCCGAGAACGGGCGAGCCGATCGAAATCAAGGCGACCACCGTGCCGGCG
TTTTCAGCATCGAAGAACTTCAAGGAACAGGAACGTGCACCGCGTCCGAA
AGTGTTGGCTCCGCGAGGGGTGCCCGGTTGCCGTGTGGTTGTACTTTTTG
TTTTTTAAAGATCAGTCCGACAGTCGTTTAAAGTACCGCATGCGGCTCGT
GCGTTGGGCTTATTATTATTATTATTATTTATTTTAGAAATGTGGCCAAT
AATGATTCTTCCACTAGGTTTGCGAAAATGACATTAAAGGCAAGGAAATG
TCACCCTAACAAGGGCTGACAAGTGACGTTCAAGGCAATTTAATGTCGAT
GACAGACTCGTAATCTCATTTCTTGTCGTCAAGTAAATGTGTGCGCTGTT
ATCTTCGCCCTCTATGACGCTGAGCTCTTGTTCGTTGTCCCTGTAATTTT
CCCGCTTGCGCCGTTATTTTTCCCTTCCATCAGGTGAAAACCAAGCACAT
CGGCTGAGCATTGCCCCCCCCCCTCCGTCGGATACTCCGCGAGTGCGGCA
CCCCATTGTTAGTTTAACGAGGCGTATATTATATATATCTGGAATGTTAG
CTGGTTCAAATGAAAAGAGGCCATTACTGCCTTCAGAAGCTCAGCGGTTC
CGAACGCGGTGGATATACCGATACCGCGTGTATGTTTAGGCAAGATGGTA
CCGATAGAGAAATTATCTGGAATCGGTGATGGAGGTTCGAAGCCAGGTCC
TTCTCTTGAGGCAAGGCGATTCGGGCATCAAACGGTCCCACAGCACGTAG
GATACACTGTACAAGTGCCGTGAGCTGGGAATCTAGCTTCCGTGGTCTGT
TCATGCTTTCAGCGTCAGTTCTCTATTTCTTCAGTTAACATGAGGGCTTT
CGCTTGTTCAAATTATTGGTCGATCCCACCCGTGACATGGATTGAATATT
ATGTCGTCTGGTAACGGTCTCACACCAAGAGGGTGCGTTTGTTATGAGAG
GTCGTCGTAGGGTGTTGAAATATGAACAAAGAAAGCCCCTTCTAGGTCTG
AAGATGGTAACCAGAGTTAAATACGCACGGATCGTTGGAGACGACAAAAG
TTGACGTGGTGGTTAGAGCTACCGATGTTACCTTAAGAGATAGGGGTCAC
AATGTTGTTCTCTCGCATAGGGAAATTATCTACAAGAGATCTTTACCACG
AACTAGGGGTTCGGTTGTTGCGGGACTGAATCCTGTGGGCAGTCATGCGG
GCGAAGATACAGACGAAAATACAAGTCCGGACGCAGACGCACCAATGACA
GCTTATACATAACCTCTACAGTAACATGAAACGGTGCATGCTAAACTATG
ACGC
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Coding sequence (CDS) from alignment at H-elongata_contig58834:197..2050-

>mRNA_H-elongata_contig58834.13384.1 ID=mRNA_H-elongata_contig58834.13384.1|Name=mRNA_H-elongata_contig58834.13384.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=894bp|location=Sequence derived from alignment at H-elongata_contig58834:197..2050- (Himanthalia elongata Himel1 dioecious)
ATGATGGAGAGAGCCGGCCTCAACAAAACGCAGGCGGAAGCAGCCGTGGT
CGCGTTCACGGAGACTATCGTCGATTTCGTGTCTGATGATGGAGAGAGCC
GGCCTCAACAAAACGCAGGCGGAAGCAGCCGTGGTCGCGTTCACGGAGAC
TATCGTCGATTTCGTGTCTGATGGGAATAAGATCAACTTGCAGGGGTTCG
GCACGTTTGAGTCTCGCCAGAGGGCGGGTCGGCAGGGCCGCAACCCGAGA
ACGGGCGAGCCGATCGAAATCAAGGCGACCACCGTGCCGGCGTTTTCAGC
ATCGAAGAACTTCAAGGAACAGGAACATGGGAATAAGATCAACTTGCAGG
GGTTCGGCACGTTTGAGTCTCGCCAGAGGGCGGGTCGGCAGGGCCGCAAC
CCGAGAACGGGCGAGCCGATCGAAATCAAGGCGACCACCGTGCCGGCGTT
TTCAGCATCGAAGAACTTCAAGGAACAGGAACGTGAAAACCAAGCACATC
GGCTGAGCATTGCCCCCCCCCCTCCGTCGGATACTCCGCGAGTGCGGCAC
CCCATTGTTAGTTTAACGAGGCGTATATTATATATATCTGGAATGTTAGC
TGGTTCAAATGAAAAGAGGCCATTACTGCCTTCAGAAGCTCAGCGGTTCC
GAACGCGGTGGATATACCGATACCGCGTGTATGTTTAGGTGAAAACCAAG
CACATCGGCTGAGCATTGCCCCCCCCCCTCCGTCGGATACTCCGCGAGTG
CGGCACCCCATTGTTAGTTTAACGAGGCGTATATTATATATATCTGGAAT
GTTAGCTGGTTCAAATGAAAAGAGGCCATTACTGCCTTCAGAAGCTCAGC
GGTTCCGAACGCGGTGGATATACCGATACCGCGTGTATGTTTAG
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