mRNA_H-elongata_contig50827.12339.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig50827.12339.1
Unique NamemRNA_H-elongata_contig50827.12339.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A6H5KEM1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEM1_9PHAE)

HSP 1 Score: 118 bits (295), Expect = 1.820e-29
Identity = 53/69 (76.81%), Postives = 59/69 (85.51%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNVKV 207
            VARY+P GYFKLHTDHVE FN L CGGRLGTLILYLND F GGQTDFP + V+V P AGDAIYFH+V++
Sbjct:  446 VARYRPDGYFKLHTDHVEDFNSLVCGGRLGTLILYLNDDFTGGQTDFPFVHVAVAPFAGDAIYFHSVQL 514          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: D7FP64_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FP64_ECTSI)

HSP 1 Score: 117 bits (293), Expect = 4.490e-29
Identity = 53/69 (76.81%), Postives = 59/69 (85.51%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNVKV 207
            VARY+P GYFKLHTDHVE FN L CGGRLGTLILYLND F GGQTDFP + V+V P AGDAIYFH+V++
Sbjct:  595 VARYRPDGYFKLHTDHVEDFNRLVCGGRLGTLILYLNDDFTGGQTDFPFVHVAVAPFAGDAIYFHSVEL 663          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A7S3Y6W7_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y6W7_HETAK)

HSP 1 Score: 83.2 bits (204), Expect = 1.170e-17
Identity = 37/69 (53.62%), Postives = 49/69 (71.01%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNVKV 207
            V RY+    + LHTDH++ FN L  GGRL T ++YLND F GG+TDFP L VSV P  G A++++NVK+
Sbjct:  156 VIRYQLNQQYTLHTDHMDEFNELEVGGRLATCLMYLNDDFTGGETDFPELKVSVSPKKGSALFWYNVKL 224          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A520HH70_SPHSX (2OG-Fe(II) oxygenase (Fragment) n=1 Tax=Sphingomonas sp. TaxID=28214 RepID=A0A520HH70_SPHSX)

HSP 1 Score: 65.1 bits (157), Expect = 4.790e-11
Identity = 32/67 (47.76%), Postives = 43/67 (64.18%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            V RY P   FKLH+D +    G     R+ T+++YLNDAF GG+T FP  G++V P AGDA+ F N+
Sbjct:  131 VLRYAPGQQFKLHSDVI----GRTRNERVATMLVYLNDAFEGGETTFPDHGLTVTPRAGDALLFRNI 193          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A7S2KU42_9STRA (Hypothetical protein n=2 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2KU42_9STRA)

HSP 1 Score: 65.5 bits (158), Expect = 7.680e-11
Identity = 31/81 (38.27%), Postives = 46/81 (56.79%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDA---------------FVGGQTDFPMLGVSVDPTAGDAIYFHN 198
            V RY+P   F++HTDH+++FN + CGGRL T ++YLND+               F GG+T F     +V P  G A+++ N
Sbjct:  239 VVRYQPGQEFQIHTDHLDSFNDMDCGGRLATCLMYLNDSEDNVPEESLSNNDETFTGGETFFHEFDRAVSPRKGSALFWWN 319          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A196ND86_9SPHN (Fe2OG dioxygenase domain-containing protein n=1 Tax=Sphingomonas sp. TDK1 TaxID=453247 RepID=A0A196ND86_9SPHN)

HSP 1 Score: 64.3 bits (155), Expect = 1.780e-10
Identity = 31/67 (46.27%), Postives = 40/67 (59.70%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            V RY P   ++ H D +          R+ T I+YLN  FVGG+TDFP LGV+V P+AGDAI F  +
Sbjct:  223 VLRYSPGQQYRRHLDTIAG----AANQRVATFIVYLNQGFVGGETDFPALGVTVRPSAGDAILFETL 285          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A0C2MRX7_THEKT (Prolyl 4-hydroxylase subunit alpha-1 n=1 Tax=Thelohanellus kitauei TaxID=669202 RepID=A0A0C2MRX7_THEKT)

HSP 1 Score: 61.2 bits (147), Expect = 8.780e-10
Identity = 29/74 (39.19%), Postives = 45/74 (60.81%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVE----AFNGLCC---GGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            +A Y   GY+  H D+      + N       GGRL T +LY++D  +GG+T FP LG+SV P+ GDA++++N+
Sbjct:   79 LANYGIGGYYDTHFDYARKTEPSINDTLTRRNGGRLATYLLYMSDVTIGGKTGFPRLGISVQPSKGDALFWYNL 152          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A3S0EJZ5_9SPHN (2OG-Fe(II) oxygenase n=1 Tax=Sphingomonas ginkgonis TaxID=2315330 RepID=A0A3S0EJZ5_9SPHN)

HSP 1 Score: 62.0 bits (149), Expect = 9.340e-10
Identity = 27/67 (40.30%), Postives = 41/67 (61.19%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            V RY+P   FK H D++   +    G R+ T +L+LN+ + GG+T+FP +GV      GDA++F NV
Sbjct:  159 VLRYRPGEQFKAHVDYLGPASPAADGQRIATFLLFLNEDYAGGETEFPSIGVRHRGRTGDALFFANV 225          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: A0A0Q4I1C2_9SPHN (Fe2OG dioxygenase domain-containing protein n=2 Tax=Sphingomonas sp. Leaf22 TaxID=1735687 RepID=A0A0Q4I1C2_9SPHN)

HSP 1 Score: 60.1 bits (144), Expect = 5.650e-9
Identity = 30/67 (44.78%), Postives = 41/67 (61.19%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            V RY P   F+LH+D +          R+ T++LYLND FVGG+T FP LG+++ P   DA+ F NV
Sbjct:  204 VLRYAPGQEFRLHSDAIAGARNQ----RIATMLLYLNDDFVGGETVFPDLGLTIRPKRCDALLFGNV 266          
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Match: UPI0009EC8EBB (2OG-Fe(II) oxygenase n=1 Tax=unclassified Sphingomonas TaxID=196159 RepID=UPI0009EC8EBB)

HSP 1 Score: 59.7 bits (143), Expect = 8.280e-9
Identity = 30/67 (44.78%), Postives = 41/67 (61.19%), Query Frame = 1
Query:    1 VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPMLGVSVDPTAGDAIYFHNV 201
            + RY+    F+LH+D + A        R  T+I+YLN+ F+GG+T FP+L V V P  GDAI F NV
Sbjct:  222 ILRYQVGQQFRLHSDALPASKNQ----RTKTVIIYLNNDFIGGETVFPILNVKVSPVMGDAIIFSNV 284          
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig50827.12339.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KEM1_9PHAE1.820e-2976.81Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FP64_ECTSI4.490e-2976.81Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S3Y6W7_HETAK1.170e-1753.62Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A520HH70_SPHSX4.790e-1147.762OG-Fe(II) oxygenase (Fragment) n=1 Tax=Sphingomon... [more]
A0A7S2KU42_9STRA7.680e-1138.27Hypothetical protein n=2 Tax=Skeletonema marinoi T... [more]
A0A196ND86_9SPHN1.780e-1046.27Fe2OG dioxygenase domain-containing protein n=1 Ta... [more]
A0A0C2MRX7_THEKT8.780e-1039.19Prolyl 4-hydroxylase subunit alpha-1 n=1 Tax=Thelo... [more]
A0A3S0EJZ5_9SPHN9.340e-1040.302OG-Fe(II) oxygenase n=1 Tax=Sphingomonas ginkgoni... [more]
A0A0Q4I1C2_9SPHN5.650e-944.78Fe2OG dioxygenase domain-containing protein n=2 Ta... [more]
UPI0009EC8EBB8.280e-944.782OG-Fe(II) oxygenase n=1 Tax=unclassified Sphingom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig50827contigH-elongata_contig50827:3860..4066 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score118.2
Seed ortholog evalue1.5e-24
Seed eggNOG ortholog2880.D7FP64
KEGG rclassRC00478
KEGG koko:K00472
KEGG ReactionR01252
KEGG Pathwayko00330,ko01100,map00330,map01100
EggNOG free text desc.procollagen-proline 4-dioxygenase activity
EggNOG OGsKOG1591@1,KOG1591@2759
EC1.14.11.2
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Exons1
Model size207
Cds size207
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930834.333712-CDS-H-elongata_contig50827:3859..40661622930834.333712-CDS-H-elongata_contig50827:3859..4066Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50827 3860..4066 +
1691679590.463469-CDS-H-elongata_contig50827:3859..40661691679590.463469-CDS-H-elongata_contig50827:3859..4066Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50827 3860..4066 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig50827.12339.1prot_H-elongata_contig50827.12339.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig50827 3860..4066 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig50827.12339.1

>prot_H-elongata_contig50827.12339.1 ID=prot_H-elongata_contig50827.12339.1|Name=mRNA_H-elongata_contig50827.12339.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=69bp
VARYKPQGYFKLHTDHVEAFNGLCCGGRLGTLILYLNDAFVGGQTDFPML
GVSVDPTAGDAIYFHNVKV
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mRNA from alignment at H-elongata_contig50827:3860..4066+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig50827.12339.1 ID=mRNA_H-elongata_contig50827.12339.1|Name=mRNA_H-elongata_contig50827.12339.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=207bp|location=Sequence derived from alignment at H-elongata_contig50827:3860..4066+ (Himanthalia elongata Himel1 dioecious)
GTCGCGCGGTACAAGCCGCAGGGCTACTTCAAGCTCCACACGGACCATGT TGAAGCGTTCAACGGCCTCTGCTGCGGCGGGCGTCTCGGTACCCTGATCC TCTACCTCAACGACGCCTTCGTGGGGGGGCAAACAGACTTCCCGATGCTC GGGGTCTCCGTCGACCCCACGGCTGGGGACGCCATATACTTTCACAACGT CAAGGTC
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Coding sequence (CDS) from alignment at H-elongata_contig50827:3860..4066+

>mRNA_H-elongata_contig50827.12339.1 ID=mRNA_H-elongata_contig50827.12339.1|Name=mRNA_H-elongata_contig50827.12339.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=414bp|location=Sequence derived from alignment at H-elongata_contig50827:3860..4066+ (Himanthalia elongata Himel1 dioecious)
GTCGCGCGGTACAAGCCGCAGGGCTACTTCAAGCTCCACACGGACCATGT
TGAAGCGTTCAACGGCCTCTGCTGCGGCGGGCGTCTCGGTACCCTGATCC
TCTACCTCAACGACGCCTTCGTGGGGGGGCAAACAGACTTCCCGATGCTC
GGGGTCTCCGTCGACCCCACGGCTGGGGACGCCATATACTTTCACAACGT
CAAGGTCGTCGCGCGGTACAAGCCGCAGGGCTACTTCAAGCTCCACACGG
ACCATGTTGAAGCGTTCAACGGCCTCTGCTGCGGCGGGCGTCTCGGTACC
CTGATCCTCTACCTCAACGACGCCTTCGTGGGGGGGCAAACAGACTTCCC
GATGCTCGGGGTCTCCGTCGACCCCACGGCTGGGGACGCCATATACTTTC
ACAACGTCAAGGTC
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