mRNA_H-elongata_contig49526.12143.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig49526.12143.1
Unique NamemRNA_H-elongata_contig49526.12143.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: D8LKY8_ECTSI (Pleckstrin homology (PH) domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LKY8_ECTSI)

HSP 1 Score: 237 bits (605), Expect = 5.450e-69
Identity = 132/213 (61.97%), Postives = 155/213 (72.77%), Query Frame = 1 
Query:   16 DCTKIFANAKQLANFNRQFLLDLRRAYGGP---SQWDLVSPVFRDS--GNWRRTTPERNGHEDPGSVPSTLSTYTSAMDNASKLEGDPFRPSVVAEDVDKGPRQMLEVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKIDAD 639
            DC +IFAN  +LA+FNRQFLLDLR+A+GGP       +VSPV   +    WR+ T       +   +                  GDP RP+      +KGPRQML+VFLKSAPFLKMYS YVNNFD+A ERLTALERDN+SF AFLTACEKQKPCRGL LRDFL+LPVQRIPRYRLLLETILQ+T+I+S+MHS VEESL+TMGGIASKI++D
Sbjct:   76 DCKEIFANTLELAHFNRQFLLDLRKAHGGPFVPGSVGVVSPVSTSTECNEWRKVTSAGRDCLEGEDI------------------GDPLRPTS-PNISEKGPRQMLQVFLKSAPFLKMYSEYVNNFDTARERLTALERDNSSFSAFLTACEKQKPCRGLHLRDFLVLPVQRIPRYRLLLETILQYTSIDSNMHSAVEESLSTMGGIASKINSD 269
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: W7TL77_9STRA (Pleckstrin domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TL77_9STRA)

HSP 1 Score: 98.6 bits (244), Expect = 3.260e-20
Identity = 50/117 (42.74%), Postives = 72/117 (61.54%), Query Frame = 1 
Query:  289 DVDKGPRQMLEVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKIDAD 639
            ++ +G +     F K+APF KMYS YV+N+D A E L  L      F AF+ ACE Q+ C+GL LR +LI+P+QRIPRY+LLL  +L+HT  E      +E ++  + G+   I+ D
Sbjct:  118 ELQRGSQAFGGFFKKAAPFFKMYSSYVSNYDRAMETLLNLIETRLDFAAFVRACELQEGCKGLNLRAYLIMPIQRIPRYKLLLTELLKHTPEEHPDFEDLEAAVKLVSGVVGAINED 234
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: L8HC96_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HC96_ACACA)

HSP 1 Score: 93.6 bits (231), Expect = 1.420e-18
Identity = 44/105 (41.90%), Postives = 71/105 (67.62%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            ++F +   FLK+Y+ Y+NNFD++   L  L++ NA+F A+L+  E +K C GL++  FL++PVQRIPRY LLL+  L+HT  +   H+ V ++L  M  +A+ I+
Sbjct:  135 DIFKQFTSFLKVYTSYINNFDNSITTLAKLKQSNANFSAYLSYAESRKECNGLQINSFLVMPVQRIPRYVLLLKDFLRHTPEDHDDHANVNKALADMQQVAAYIE 239
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: A0A6P8IIR1_ACTTE (FYVE, RhoGEF and PH domain-containing protein 6-like n=1 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8IIR1_ACTTE)

HSP 1 Score: 91.3 bits (225), Expect = 9.960e-18
Identity = 46/105 (43.81%), Postives = 67/105 (63.81%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            + FL  APF+K+YS Y NNF+ A+  L   E+ +  FV+F+T  E  + C+GL LR  LI PVQR+PRY+LLLE++L  T  +    SK+EE+   +  +A  I+
Sbjct:  120 DAFLAMAPFIKLYSTYANNFERASNMLQEWEKKSQDFVSFITRQEAMQECKGLNLRALLITPVQRVPRYKLLLESLLNKTPRDYPDFSKLEEATEEIAKVAEHIN 224
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: A0A835YJ47_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJ47_9STRA)

HSP 1 Score: 91.3 bits (225), Expect = 1.140e-17
Identity = 51/123 (41.46%), Postives = 80/123 (65.04%), Query Frame = 1 
Query:  265 FRPSVVAEDVDKGPRQMLEVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            F  S+ AE   K  +++  VF +SA F KMYS+Y+ N+D A +R+  LE  +  F AF+ ACE Q+ CRGL LR +LI PVQR+PRY+LLL  +L+HT      ++ +  +L+ +  +A++++
Sbjct:  102 FLQSLQAEHAQK--KRLGIVFKESASFFKMYSMYIVNYDEAMKRVRLLE-GSGEFRAFVRACELQEACRGLDLRSYLIQPVQRVPRYKLLLAELLKHTKETHPDYANISMALSEITVVATRLN 221
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: L8GKC4_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GKC4_ACACA)

HSP 1 Score: 90.1 bits (222), Expect = 2.820e-17
Identity = 50/134 (37.31%), Postives = 81/134 (60.45%), Query Frame = 1 
Query:  247 KLEGDP-FRPSVVAED--VDKGPRQMLEVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKIDAD 639
            KL+  P F  S    D    K    +L  F+   PF KMY++Y+N+FD+A +R+T L+  +  F  FL  C+ +  C+ L L  FLI+P+QR+PRY LLL  IL++TA ++S H+K+E+   T+  +   ++++
Sbjct:  248 KLDKRPSFYDSATKSDSKTQKSLGDLLRAFI---PFFKMYTVYINSFDNANKRITDLQNSSKKFTQFLNDCKARPECKKLDLVSFLIMPIQRLPRYELLLRDILRNTA-DASEHAKIEQVFNTVAEVNKHVNSE 377
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: UPI00109BAF0D (rho guanine nucleotide exchange factor 39-like n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI00109BAF0D)

HSP 1 Score: 89.0 bits (219), Expect = 6.130e-17
Identity = 45/105 (42.86%), Postives = 66/105 (62.86%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            + FL  APF+K+YS Y NNF+ A+  L   ER +  FV F+   E ++ C+GL LR  LI PVQR+PRY++LLE++L  T  +    SK+EE+   +  +A  I+
Sbjct:  122 DAFLAMAPFIKLYSTYANNFEHASRILQEWERKSPEFVQFIXRQEAKEECKGLNLRSLLITPVQRVPRYKMLLESLLNKTPRDYPDFSKLEEATEEIAKVAEHIN 226
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: UPI00138FF88E (FYVE, RhoGEF and PH domain-containing protein 2 n=1 Tax=Nematostella vectensis TaxID=45351 RepID=UPI00138FF88E)

HSP 1 Score: 88.2 bits (217), Expect = 1.180e-16
Identity = 45/105 (42.86%), Postives = 67/105 (63.81%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            + FL  APF+K+YS Y NNF++A+  L   E+ +  F AF+T  E  + C+GL +R  LI PVQR+PRY+LLLE++L  T  +    SK+EE+   +  +A  I+
Sbjct:  189 DAFLAMAPFIKLYSTYANNFEAASRLLQEWEKKSPEFSAFITRQEALEECQGLNIRALLITPVQRVPRYKLLLESLLNKTPRDYPDFSKLEEATYEIAKVAEHIN 293
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: UPI001BB19EF8 (rho guanine nucleotide exchange factor 39-like isoform X1 n=2 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB19EF8)

HSP 1 Score: 87.8 bits (216), Expect = 1.550e-16
Identity = 43/105 (40.95%), Postives = 65/105 (61.90%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            E FL+ AP++K+YS Y NNF+ A + L   E   A+FV FL   EK+  CR L L   LI P+QR+PRY+LLL+ +L HT      ++K++++L  +  +   I+
Sbjct:  132 EAFLQLAPYIKLYSTYANNFEKAIDLLQEWESKAANFVRFLRETEKKDKCRSLSLHALLITPIQRVPRYKLLLKQLLDHTPSSHQDYTKIKDALQNISEVTHGIN 236
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BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Match: A0A397TNA4_9GLOM (Uncharacterized protein n=1 Tax=Glomus cerebriforme TaxID=658196 RepID=A0A397TNA4_9GLOM)

HSP 1 Score: 87.4 bits (215), Expect = 2.290e-16
Identity = 44/105 (41.90%), Postives = 68/105 (64.76%), Query Frame = 1 
Query:  319 EVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTACEKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESLTTMGGIASKID 633
            ++FL  APF KMYS+YV NF+SA   +    RDN +F AFL    K   C+GL L+ +LI+PVQRIPRY+LLLE +L+ T +    +  ++++   +  +A+ ++
Sbjct:  426 DIFLDMAPFFKMYSIYVKNFNSALSVINVQLRDNPAFSAFLRDIIKTGQCKGLTLQAYLIMPVQRIPRYKLLLEDLLKKTVVTHPDYLNLKKAYQVIENVATFVN 530
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig49526.12143.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LKY8_ECTSI5.450e-6961.97Pleckstrin homology (PH) domain-containing protein... [more]
W7TL77_9STRA3.260e-2042.74Pleckstrin domain-containing protein n=2 Tax=Monod... [more]
L8HC96_ACACA1.420e-1841.90RhoGEF domain containing protein n=1 Tax=Acanthamo... [more]
A0A6P8IIR1_ACTTE9.960e-1843.81FYVE, RhoGEF and PH domain-containing protein 6-li... [more]
A0A835YJ47_9STRA1.140e-1741.46Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
L8GKC4_ACACA2.820e-1737.31RhoGEF domain containing protein n=1 Tax=Acanthamo... [more]
UPI00109BAF0D6.130e-1742.86rho guanine nucleotide exchange factor 39-like n=1... [more]
UPI00138FF88E1.180e-1642.86FYVE, RhoGEF and PH domain-containing protein 2 n=... [more]
UPI001BB19EF81.550e-1640.95rho guanine nucleotide exchange factor 39-like iso... [more]
A0A397TNA4_9GLOM2.290e-1641.90Uncharacterized protein n=1 Tax=Glomus cerebriform... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig49526contigH-elongata_contig49526:2236..4003 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score238.8
Seed ortholog evalue2.3e-60
Seed eggNOG ortholog2880.D8LKY8
EggNOG free text desc.Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
EggNOG OGsCOG5422@1,KOG4424@2759
Ec32 ortholog descriptionPleckstrin homology domain
Ec32 orthologEc-11_006060.1
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons3
Model size639
Cds size639
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig49526.12143.1prot_H-elongata_contig49526.12143.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig49526 2236..4003 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930814.563229-CDS-H-elongata_contig49526:2235..25861622930814.563229-CDS-H-elongata_contig49526:2235..2586Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 2236..2586 +
1691679581.7223988-CDS-H-elongata_contig49526:2235..25861691679581.7223988-CDS-H-elongata_contig49526:2235..2586Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 2236..2586 +
1622930814.5758502-CDS-H-elongata_contig49526:3180..33391622930814.5758502-CDS-H-elongata_contig49526:3180..3339Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 3181..3339 +
1691679581.733265-CDS-H-elongata_contig49526:3180..33391691679581.733265-CDS-H-elongata_contig49526:3180..3339Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 3181..3339 +
1622930814.590483-CDS-H-elongata_contig49526:3874..40031622930814.590483-CDS-H-elongata_contig49526:3874..4003Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 3875..4003 +
1691679581.741306-CDS-H-elongata_contig49526:3874..40031691679581.741306-CDS-H-elongata_contig49526:3874..4003Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49526 3875..4003 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig49526.12143.1

>prot_H-elongata_contig49526.12143.1 ID=prot_H-elongata_contig49526.12143.1|Name=mRNA_H-elongata_contig49526.12143.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=213bp
MSPKPDCTKIFANAKQLANFNRQFLLDLRRAYGGPSQWDLVSPVFRDSGN
WRRTTPERNGHEDPGSVPSTLSTYTSAMDNASKLEGDPFRPSVVAEDVDK
GPRQMLEVFLKSAPFLKMYSLYVNNFDSATERLTALERDNASFVAFLTAC
EKQKPCRGLRLRDFLILPVQRIPRYRLLLETILQHTAIESSMHSKVEESL
TTMGGIASKIDAD
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mRNA from alignment at H-elongata_contig49526:2236..4003+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig49526.12143.1 ID=mRNA_H-elongata_contig49526.12143.1|Name=mRNA_H-elongata_contig49526.12143.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1768bp|location=Sequence derived from alignment at H-elongata_contig49526:2236..4003+ (Himanthalia elongata Himel1 dioecious)
ATGTCGCCCAAACCAGACTGCACCAAGATCTTCGCCAACGCCAAGCAGCT
GGCAAACTTTAATCGGCAGTTTCTTCTGGACCTCCGAAGGGCTTATGGAG
GTCCTAGCCAGTGGGACCTCGTATCTCCTGTGTTTAGAGACTCCGGGAAC
TGGAGACGGACCACCCCGGAGAGGAACGGGCATGAGGATCCTGGCAGTGT
CCCGTCGACGCTCTCCACGTACACCTCGGCGATGGATAACGCGAGCAAGC
TAGAGGGTGATCCGTTCCGACCGTCGGTGGTCGCAGAGGATGTAGATAAG
GGTCCGCGGCAGATGCTAGAGGTTTTCCTGAAGTCGGCGCCCTTCCTCAA
GGTGCGGAGATTGATGCGGATTAGGGTACAAATACTATCTCATATTTGAT
TTGATTTGATTTCGTTTGCCATTAGTAACTCGACTGCCCATCTGGAATTT
GTCTTTGTTATCGGTGTCCTCGTTTGTCATAGGATATGTTTTGTCGTTGG
TTTACTCGATGCGCGCTGTCTTAGCGTCAAGCAGGACTCTCGCGGGCGCG
TGTTGCCCTTGGTCATGTGTGCTCGCCAATAGAGCAGGAGAGCCTGCTGT
GTTCTGTGTGCTACTTTGTGACGTGCTTTTAGCTCTTTCTACTAGACCAG
ACCATACGTCACGTCATGTCACGCCATGTCACATTGTTGGATGTAAATAA
TTAAAAGCAAAAGCGAAAAATGCGGAACTTATCTATCCTAATGGTCAAGG
TTACCGGGCATCCGGGAGTCTTTTTTACGATACGATGTTGCCATGTCGTA
GCCACGCTGCATTTGTCGACGAACTCTTAAATACCTGATTATACATTATC
GGAATCAATGTCTCCTCCGCCCTCGCCGATCGATCAATGTATGGATGTAT
GTGGGGGGATGTATGGATATGTTCCCTCGCGGTGGCGGCGTGTAGATGTA
TTCCTTGTACGTGAACAACTTCGACAGTGCGACGGAGCGTTTGACGGCGT
TAGAGAGAGATAACGCCTCCTTCGTAGCCTTCCTCACGGCGTGCGAGAAG
CAGAAGCCTTGCCGCGGCCTTCGCCTTCGAGATTTTCTCATCCTACCTGT
GCAGGTGGGAAATAGTATTTTGAATATATTGAAAATAGTATAGTCTAGTG
TTGCTAATGGAATATCCTGGAAGCGTGAGCATCTCTGGAATATCACTGAT
GTCTGAGCCGGTCATCCTCTTCCTTTCCCCACGTGTAAGCTATTGAATCG
ATCGATCGATCAGAGGTGTGTCGACTATTTACCTCCGTTTCTGCCGCCGA
TTTTCCAAGGGCAGGGTAGGGCAACCGGTTGCGCAAAAAGTCGAAATGAC
TGGATAGCACTTAAAAAAAAAATCTAGTAAATCTACAGAGAAGTTTTGAG
TTTAGTTTTTGGCATGAAAGTCAGTAGAGCGAAAACTTTTGTTTTTTTTC
GGCGCCCTGAACCCCCCTCCCCCCGACACACGCCTCCCTCTCTAACCCAA
ACTTCTAGAAGGTCTTTTGAAGGGTGCAGTATTCGACATTGCTCCTATTA
TAATATTAGAATAGTGTACTCTAGACTGAGCTCGAGCAATCCCTGCGTGC
TGCGTGTGTGTGTAACTGTACGGTAACTATACGTTGCAGCGGATCCCTCG
GTATAGGCTTCTCCTAGAGACGATTCTGCAGCACACGGCGATCGAGAGCT
CCATGCATTCCAAGGTGGAGGAGAGCCTGACCACGATGGGAGGTATCGCT
TCCAAGATTGACGCCGAC
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Coding sequence (CDS) from alignment at H-elongata_contig49526:2236..4003+

>mRNA_H-elongata_contig49526.12143.1 ID=mRNA_H-elongata_contig49526.12143.1|Name=mRNA_H-elongata_contig49526.12143.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1278bp|location=Sequence derived from alignment at H-elongata_contig49526:2236..4003+ (Himanthalia elongata Himel1 dioecious)
ATGTCGCCCAAACCAGACTGCACCAAGATCTTCGCCAACGCCAAGCAGCT
GGCAAACTTTAATCGGCAGTTTCTTCTGGACCTCCGAAGGGCTTATGGAG
GTCCTAGCCAGTGGGACCTCGTATCTCCTGTGTTTAGAGACTCCGGGAAC
TGGAGACGGACCACCCCGGAGAGGAACGGGCATGAGGATCCTGGCAGTGT
CCCGTCGACGCTCTCCACGTACACCTCGGCGATGGATAACGCGAGCAAGC
TAGAGGGTGATCCGTTCCGACCGTCGGTGGTCGCAGAGGATGTAGATAAG
GGTCCGCGGCAGATGCTAGAGGTTTTCCTGAAGTCGGCGCCCTTCCTCAA
GATGTCGCCCAAACCAGACTGCACCAAGATCTTCGCCAACGCCAAGCAGC
TGGCAAACTTTAATCGGCAGTTTCTTCTGGACCTCCGAAGGGCTTATGGA
GGTCCTAGCCAGTGGGACCTCGTATCTCCTGTGTTTAGAGACTCCGGGAA
CTGGAGACGGACCACCCCGGAGAGGAACGGGCATGAGGATCCTGGCAGTG
TCCCGTCGACGCTCTCCACGTACACCTCGGCGATGGATAACGCGAGCAAG
CTAGAGGGTGATCCGTTCCGACCGTCGGTGGTCGCAGAGGATGTAGATAA
GGGTCCGCGGCAGATGCTAGAGGTTTTCCTGAAGTCGGCGCCCTTCCTCA
AGATGTATTCCTTGTACGTGAACAACTTCGACAGTGCGACGGAGCGTTTG
ACGGCGTTAGAGAGAGATAACGCCTCCTTCGTAGCCTTCCTCACGGCGTG
CGAGAAGCAGAAGCCTTGCCGCGGCCTTCGCCTTCGAGATTTTCTCATCC
TACCTGTGCAGATGTATTCCTTGTACGTGAACAACTTCGACAGTGCGACG
GAGCGTTTGACGGCGTTAGAGAGAGATAACGCCTCCTTCGTAGCCTTCCT
CACGGCGTGCGAGAAGCAGAAGCCTTGCCGCGGCCTTCGCCTTCGAGATT
TTCTCATCCTACCTGTGCAGCGGATCCCTCGGTATAGGCTTCTCCTAGAG
ACGATTCTGCAGCACACGGCGATCGAGAGCTCCATGCATTCCAAGGTGGA
GGAGAGCCTGACCACGATGGGAGGTATCGCTTCCAAGATTGACGCCGACC
GGATCCCTCGGTATAGGCTTCTCCTAGAGACGATTCTGCAGCACACGGCG
ATCGAGAGCTCCATGCATTCCAAGGTGGAGGAGAGCCTGACCACGATGGG
AGGTATCGCTTCCAAGATTGACGCCGAC
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