mRNA_H-elongata_contig47238.11832.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig47238.11832.1
Unique NamemRNA_H-elongata_contig47238.11832.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Match: D7G861_ECTSI (ADP-ribosylation factor GTPase-activating protein, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G861_ECTSI)

HSP 1 Score: 238 bits (606), Expect = 2.660e-67
Identity = 165/271 (60.89%), Postives = 186/271 (68.63%), Query Frame = 1 
Query:    1 RQLTMDCWDIAQVTRMELGGNKQLRAWFARCKTENSALEMKYRTKAATLYRANLHAAAEEEIARRKAGGKEMARSAEGGRTEAGKGRIYAASKATATRDLARERENAVVAXXXXXXXXXXXXXXXXXXXRGADGSTGVVDPLLA----------SGAADSAVVTVPDVSSLTGSGEGARRCGNEE-YEVVFGAGGLGFTLMKDAVSRALVTRLAPGGAAFHLGVRTGDYLVGVSGNRLFDYDKLMVLLPRAPRPLRLLFEGAGAALAAPPP 780
            + L MD WD  QV RMELGGN QL+AWF +C+TENSALEMKYRTKAATLYR NL  AA+E++A   +GG + A   +G    + KGRI+AA++  A   LA+E       XXXXXXXXXXXXXX     R    S+    P+ A          +GAA         V S  G  E ARRCG EE YEVVFG GG+GFTLMKDAVSRALVTRLAPGG AF LGVRTGDYLVGV G RL DYD+LM+LLPR PRPLRLLFEGAGAALAA PP
Sbjct:   38 KSLAMDRWDAGQVRRMELGGNGQLQAWFTKCQTENSALEMKYRTKAATLYRENLRVAADEDLA---SGGGQAAGELQGA-PGSRKGRIFAAAERAAASSLAQEEAXXXXXXXXXXXXXXXXXXXRREAERAGALSSAAQVPVAARHDPPSAHAGAGAAPGGATAAATVGS--GMEEYARRCGREEEYEVVFGPGGMGFTLMKDAVSRALVTRLAPGGMAFRLGVRTGDYLVGVDGRRLHDYDQLMLLLPRTPRPLRLLFEGAGAALAASPP 302
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BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Match: A0A835ZEM7_9STRA (Arf-GAP domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEM7_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 1.020e-11
Identity = 31/54 (57.41%), Postives = 40/54 (74.07%), Query Frame = 1 
Query:    1 RQLTMDCWDIAQVTRMELGGNKQLRAWFARCKTENSALEMKYRTKAATLYRANL 162
            R LT+D W+   +  ++LGGN QL +W  RCK +NSA+E+KYRTKAATLYR  L
Sbjct:   18 RSLTLDRWEERHIASLKLGGNAQLHSWLTRCKVQNSAIEVKYRTKAATLYREKL 71
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BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Match: A0A7R9U3N9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U3N9_9STRA)

HSP 1 Score: 60.8 bits (146), Expect = 8.460e-7
Identity = 40/115 (34.78%), Postives = 64/115 (55.65%), Query Frame = 1 
Query:  439 AADSAVVTVPDVSS--LTGSGEGARRCGNEEYEVVFGAGGLGFTLMKDAVSRALVTRLAPGGAAFHLGVRTGDYLVGVSGNRLFDYDKLMVLLPRAPRPLRLLF------EGAGA 759
            AA +A+   P+ +S  +T  G  + R G ++    FG G +G  L +    RA V+ + P G A   G+R GD++ GV+G R+ DYD++M L+ R  RP+ + F      +GAG+
Sbjct:  134 AAAAALGDAPEAASRSVTAPGGSSGRDGVQDVWCSFGEGRMGLRLTRAKNMRATVSMITPNGNADVQGLRVGDFVCGVNGKRVGDYDEVMELVQRLKRPVSITFRRITRCDGAGS 248
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BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Match: A0A835YRV1_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRV1_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 6.730e-6
Identity = 28/73 (38.36%), Postives = 43/73 (58.90%), Query Frame = 1 
Query:  526 YEVVFGAGGLGFTLMKDAVSRALVTRLAPGGAAFHLGVRTGDYLVGVSGNRLFDYDKLMVLLPRAPRPLRLLF 744
            Y+V FG G LGFT++K      +V ++ P   A   GV  GD +V ++  R+  YD++M +LP  PRP+ + F
Sbjct:  361 YDVTFGDGRLGFTMLKSEQGLGVVCKVQPDSTAASAGVSPGDTVVAINRRRMSSYDEVMSVLPSMPRPVLVTF 433
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BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Match: A0A8J2SXS4_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SXS4_9STRA)

HSP 1 Score: 56.6 bits (135), Expect = 3.180e-5
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 1 
Query:  583 SRALVTRLAPGGAAFHLGVRTGDYLVGVSGNRLFDYDKLMVLLPRAPRPLRLLFEGAGAALAA 771
            S ALVT++   G A   GVR GDY+V ++G    DYD+++ L  RAPRPLRL     G   +A
Sbjct:  198 SMALVTKVVTNGPAHSAGVRVGDYVVALNGRPATDYDEVVALFARAPRPLRLTVRRFGGDASA 260
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig47238.11832.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D7G861_ECTSI2.660e-6760.89ADP-ribosylation factor GTPase-activating protein,... [more]
A0A835ZEM7_9STRA1.020e-1157.41Arf-GAP domain-containing protein (Fragment) n=1 T... [more]
A0A7R9U3N9_9STRA8.460e-734.78Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A835YRV1_9STRA6.730e-638.36Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A8J2SXS4_9STRA3.180e-546.03Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig47238contigH-elongata_contig47238:1274..4135 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score245.0
Seed ortholog evalue5e-62
Seed eggNOG ortholog2880.D7G861
KEGG koko:K12492,ko:K12493,ko:K14480
KEGG Pathwayko04144,map04144
EggNOG free text desc.GTPase activator activity
EggNOG OGsCOG5347@1,KOG0706@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131
Hectar predicted targeting categoryother localisation
Exons6
Model size993
Cds size981
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930779.6095967-CDS-H-elongata_contig47238:1273..14331622930779.6095967-CDS-H-elongata_contig47238:1273..1433Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 1274..1433 -
1691679566.6547132-CDS-H-elongata_contig47238:1273..14331691679566.6547132-CDS-H-elongata_contig47238:1273..1433Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 1274..1433 -
1622930779.6303182-CDS-H-elongata_contig47238:1508..16951622930779.6303182-CDS-H-elongata_contig47238:1508..1695Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 1509..1695 -
1691679566.6737523-CDS-H-elongata_contig47238:1508..16951691679566.6737523-CDS-H-elongata_contig47238:1508..1695Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 1509..1695 -
1622930779.6450775-CDS-H-elongata_contig47238:2116..22311622930779.6450775-CDS-H-elongata_contig47238:2116..2231Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 2117..2231 -
1691679566.6847563-CDS-H-elongata_contig47238:2116..22311691679566.6847563-CDS-H-elongata_contig47238:2116..2231Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 2117..2231 -
1622930779.657036-CDS-H-elongata_contig47238:2749..29711622930779.657036-CDS-H-elongata_contig47238:2749..2971Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 2750..2971 -
1691679566.69568-CDS-H-elongata_contig47238:2749..29711691679566.69568-CDS-H-elongata_contig47238:2749..2971Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 2750..2971 -
1622930779.673923-CDS-H-elongata_contig47238:3513..36701622930779.673923-CDS-H-elongata_contig47238:3513..3670Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 3514..3670 -
1691679566.7113428-CDS-H-elongata_contig47238:3513..36701691679566.7113428-CDS-H-elongata_contig47238:3513..3670Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 3514..3670 -
1622930779.6877122-CDS-H-elongata_contig47238:3983..41231622930779.6877122-CDS-H-elongata_contig47238:3983..4123Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 3984..4123 -
1691679566.7224333-CDS-H-elongata_contig47238:3983..41231691679566.7224333-CDS-H-elongata_contig47238:3983..4123Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig47238 3984..4123 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930779.7052474-UTR-H-elongata_contig47238:4123..41351622930779.7052474-UTR-H-elongata_contig47238:4123..4135Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig47238 4124..4135 -
1691679566.7343369-UTR-H-elongata_contig47238:4123..41351691679566.7343369-UTR-H-elongata_contig47238:4123..4135Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig47238 4124..4135 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig47238.11832.1prot_H-elongata_contig47238.11832.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig47238 1274..4123 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig47238.11832.1

>prot_H-elongata_contig47238.11832.1 ID=prot_H-elongata_contig47238.11832.1|Name=mRNA_H-elongata_contig47238.11832.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=327bp
MDCWDIAQVTRMELGGNKQLRAWFARCKTENSALEMKYRTKAATLYRANL
HAAAEEEIARRKAGGKEMARSAEGGRTEAGKGRIYAASKATATRDLARER
ENAVVAAAAVGALAKKKKDKTSRRRRGADGSTGVVDPLLASGAADSAVVT
VPDVSSLTGSGEGARRCGNEEYEVVFGAGGLGFTLMKDAVSRALVTRLAP
GGAAFHLGVRTGDYLVGVSGNRLFDYDKLMVLLPRAPRPLRLLFEGAGAA
LAAPPPGNSSSPLTSSRLPTLLATGADRASTRSSDSESSSSNSSSGNGSS
ISGSSSGSTTASSGGTGGRETDTADEE
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mRNA from alignment at H-elongata_contig47238:1274..4135-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig47238.11832.1 ID=mRNA_H-elongata_contig47238.11832.1|Name=mRNA_H-elongata_contig47238.11832.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2862bp|location=Sequence derived from alignment at H-elongata_contig47238:1274..4135- (Himanthalia elongata Himel1 dioecious)
CGGCAGCTGACGATGGACTGCTGGGACATTGCTCAGGTGACAAGGATGGA
GCTCGGCGGCAACAAGCAGCTTAGGGCGTGGTTCGCCAGATGCAAAACGG
AGAACAGCGCCCTGGAGATGAAGTACAGGACCAAGGCCGCCACGCTCTAC
CGGTACGTCGGCGCGTCGGTGGAGTAAAACCCAGAGCTCCGTGTTGCACC
ATTTTTATGGGCATAATGATTATGTTTTTATAGGTAACGTGAACTATCGT
GGGGATGACACATTGCCGCTCCTCCAAACGGAAGGGTCACCCCCATCCGT
TGTTCAGTGGTGCAAGTTTCAAAATTGAACAAACACTAAAGACTCTAGGT
GGGCAGAGAGGCAACGCATGCTGCAAAAAATCGTTTTAACTGTTAAAGTC
CAGGATCCAGAAGTGAGAGTGAAAACGCGCGCGCGTGTCCACGACCGTTT
GTTCGGCAACGACAGTGCCAACCTGCATGCCGCCGCGGAGGAAGAGATCG
CTCGACGCAAAGCCGGGGGCAAAGAGATGGCAAGGTCGGCAGAAGGCGGA
CGGACAGAAGCCGGAAAGGGTCGCATTTACGCCGCCTCAAAAGCAACCGC
GACAAGGGACCTCGCCCGAGAGGTGCAGTATATAAATTACAGTAGTAAAT
ACAATCCTGGAGAGGTTGAAACGGTAACGGGTGAAAACCAGGCCTCCGCG
TACTCACGCGCGGCGTTCTTTTTTCCTGTCGTGCTTGACGCCAGGACTTG
GTCGTAGCGGGGGTAGGGACCATTAATAGTACATTTTTTGTGAGGAGCGA
TTGATGGCTAAACACTCCGAAGATGTCATGTTTCGTCGTGTTTTGGCTTG
CGGTGTGTACATCTTGTATAGGACCTTGTCGGCCGACACTACGATTCCAG
CAAGGGCACAACTTGGAAATCATCCGCATCCCGATCGCTTGAAGCACACC
CTCCACTTCCACACACACACCGCCCCACTCCCCATATTTCCCCTCCTCGT
CCCCTTTCCGGGTGAGGAATTGGATCGACTTATTCCGGGTGGTACCCCAT
CATACATTCTCCATCCGCGTACGCATGTTCATTTCGAACCGAGGCGAGTC
GAGCGCACGAACGCGGCAGCACCCCACCTCTCTTTACATTCCTCGCCCTC
TCGCCTCGCTACAGAGAGAAAACGCGGTGGTGGCGGCAGCGGCGGTCGGA
GCGCTAGCCAAGAAGAAGAAGGACAAGACCAGCCGCAGACGACGGGGCGC
GGACGGGAGCACCGGCGTGGTAGATCCCTTGTTGGCGTCTGGTGCTGCTG
ATTCGGCGGTGGTGACGGTGCCGGACGTTTCGAGTTTGACTGGGAGCGGA
GAAGGGGCGAGGCGGTGCGGAAACGAAGAGTATGAGGTGTGCGGTGTGCG
GTATGCGGACGTGCGTGCTCTTGTAGAGAACTCCCACCCACCCACTCACC
CACCCCTATGAGGCAACATTTTTTTATGATGCCTGATCTCTGGACTCGGC
AAGACGTAAAACCTCGGATTTTGCTCCCTAGATTAGCCCCTTATTTGACG
ATGTAATGTGGGAGCCGAAATGACATCTTGAGAGCTTTGGGGGGAAATCC
ATCACGTTTATTTACACAATCATTTGCCCTCATTTTGGGGGATGTGTGAT
GCAACCAGGGCCTCGAAATGAGAGTTTTGAGAGTTTTTAAGGGCACAATC
ATATGCTCTCTCAGCGTTTGTATTTGTGTATAGTGGGAGTGGTCGGGGAG
AGGCTTTATCTAAAACAGTCGGGTTGCCGGTGAACGCCATATTATTGGGT
ACAGAAAATCCAATGGTGTCTTCTCCGCGCCCCCCCCCTCGAAAATGCCG
CTCTTTCCACCTATTTGTATGTGAATGTGAATTGTGTGTCTCCTGTTGCC
GGAGGTGGTCTTCGGAGCTGGAGGCTTGGGCTTCACCCTTATGAAGGACG
CTGTCAGCCGCGCACTTGTCACTCGTCTCGCCCCCGGCGGCGCTGCTTTT
CACCTCGGAGTTCGTACGGGTGCGTTTGCTTGTGTATGGTTGTGCGTCTA
CAACTGCGAGCGCGCGGACGGGTGATTGGAACTTCGCGCGTCGGACGGAA
CGATCGCCATCGACGCGACAACGGAGAACACTTCGGTGTACACACCACAC
ACACACAAACACACGGATATAGACACGTGCGAGAGTGGCACGTCCTTCTC
TTTTTCTCCCTCAAAAAAACAAGCTGGGGAGATGTTGACATGTTCTCGTT
GGGGCGTGTCACTACTCTCAAGATCTGTAGGCAAGAGCAGTATATGTATA
TGCACATATAATTGTTTTGAACAAGGCAAAAACAACCACCTCTATATGGC
CATCCTGTTCCCTGGGGACTAGCCCCTGATTCCTAAAAGCCTCCGGGCCT
TCTCGCCTCCTTTATTATGGATTACTTGACTTCGTTTCAGGTGACTATCT
TGTCGGCGTGAGCGGAAACCGCCTTTTTGACTACGACAAGCTGATGGTCC
TCCTTCCCCGGGCACCTCGGCCGCTGCGCCTGCTCTTCGAGGGCGCGGGG
GCAGCGTTAGCAGCCCCGCCGCCGGGCAATTCATCCTCTCCACTCACCTC
TTCGCGCTTGCCAACGCTGCTCGCCACGTCGCCGCCGTTTTCCCACCAGC
TTGAGAAGGAGCAGCAACGAGAACGACCACGGTGGTTGGGTCCGCGGGGG
AGCGGCGCCGACCGTGCGTCTACCCGTTCAAGCGACAGCGAATCCTCTTC
GAGCAATAGCAGTAGTGGTAACGGTAGCAGCATCAGCGGCAGCAGCAGTG
GTAGTACCACCGCTAGCAGCGGGGGAACTGGTGGCCGCGAGACGGACACT
GCTGACGAAGAG
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Coding sequence (CDS) from alignment at H-elongata_contig47238:1274..4135-

>mRNA_H-elongata_contig47238.11832.1 ID=mRNA_H-elongata_contig47238.11832.1|Name=mRNA_H-elongata_contig47238.11832.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1962bp|location=Sequence derived from alignment at H-elongata_contig47238:1274..4135- (Himanthalia elongata Himel1 dioecious)
ATGGACTGCTGGGACATTGCTCAGGTGACAAGGATGGAGCTCGGCGGCAA
CAAGCAGCTTAGGGCGTGGTTCGCCAGATGCAAAACGGAGAACAGCGCCC
TGGAGATGAAGTACAGGACCAAGGCCGCCACGCTCTACCGATGGACTGCT
GGGACATTGCTCAGGTGACAAGGATGGAGCTCGGCGGCAACAAGCAGCTT
AGGGCGTGGTTCGCCAGATGCAAAACGGAGAACAGCGCCCTGGAGATGAA
GTACAGGACCAAGGCCGCCACGCTCTACCGTGCCAACCTGCATGCCGCCG
CGGAGGAAGAGATCGCTCGACGCAAAGCCGGGGGCAAAGAGATGGCAAGG
TCGGCAGAAGGCGGACGGACAGAAGCCGGAAAGGGTCGCATTTACGCCGC
CTCAAAAGCAACCGCGACAAGGGACCTCGCCCGAGAGTGCCAACCTGCAT
GCCGCCGCGGAGGAAGAGATCGCTCGACGCAAAGCCGGGGGCAAAGAGAT
GGCAAGGTCGGCAGAAGGCGGACGGACAGAAGCCGGAAAGGGTCGCATTT
ACGCCGCCTCAAAAGCAACCGCGACAAGGGACCTCGCCCGAGAGAGAGAA
AACGCGGTGGTGGCGGCAGCGGCGGTCGGAGCGCTAGCCAAGAAGAAGAA
GGACAAGACCAGCCGCAGACGACGGGGCGCGGACGGGAGCACCGGCGTGG
TAGATCCCTTGTTGGCGTCTGGTGCTGCTGATTCGGCGGTGGTGACGGTG
CCGGACGTTTCGAGTTTGACTGGGAGCGGAGAAGGGGCGAGGCGGTGCGG
AAACGAAGAGTATGAGAGAGAAAACGCGGTGGTGGCGGCAGCGGCGGTCG
GAGCGCTAGCCAAGAAGAAGAAGGACAAGACCAGCCGCAGACGACGGGGC
GCGGACGGGAGCACCGGCGTGGTAGATCCCTTGTTGGCGTCTGGTGCTGC
TGATTCGGCGGTGGTGACGGTGCCGGACGTTTCGAGTTTGACTGGGAGCG
GAGAAGGGGCGAGGCGGTGCGGAAACGAAGAGTATGAGGTGGTCTTCGGA
GCTGGAGGCTTGGGCTTCACCCTTATGAAGGACGCTGTCAGCCGCGCACT
TGTCACTCGTCTCGCCCCCGGCGGCGCTGCTTTTCACCTCGGAGTTCGTA
CGGGTGGTCTTCGGAGCTGGAGGCTTGGGCTTCACCCTTATGAAGGACGC
TGTCAGCCGCGCACTTGTCACTCGTCTCGCCCCCGGCGGCGCTGCTTTTC
ACCTCGGAGTTCGTACGGGTGACTATCTTGTCGGCGTGAGCGGAAACCGC
CTTTTTGACTACGACAAGCTGATGGTCCTCCTTCCCCGGGCACCTCGGCC
GCTGCGCCTGCTCTTCGAGGGCGCGGGGGCAGCGTTAGCAGCCCCGCCGC
CGGGCAATTCATCCTCTCCACTCACCTCTTCGCGCTTGCCAACGCTGCTC
GCCACGTGACTATCTTGTCGGCGTGAGCGGAAACCGCCTTTTTGACTACG
ACAAGCTGATGGTCCTCCTTCCCCGGGCACCTCGGCCGCTGCGCCTGCTC
TTCGAGGGCGCGGGGGCAGCGTTAGCAGCCCCGCCGCCGGGCAATTCATC
CTCTCCACTCACCTCTTCGCGCTTGCCAACGCTGCTCGCCACCGGCGCCG
ACCGTGCGTCTACCCGTTCAAGCGACAGCGAATCCTCTTCGAGCAATAGC
AGTAGTGGTAACGGTAGCAGCATCAGCGGCAGCAGCAGTGGTAGTACCAC
CGCTAGCAGCGGGGGAACTGGTGGCCGCGAGACGGACACTGCTGACGAAG
AGCGGCGCCGACCGTGCGTCTACCCGTTCAAGCGACAGCGAATCCTCTTC
GAGCAATAGCAGTAGTGGTAACGGTAGCAGCATCAGCGGCAGCAGCAGTG
GTAGTACCACCGCTAGCAGCGGGGGAACTGGTGGCCGCGAGACGGACACT
GCTGACGAAGAG
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