mRNA_H-elongata_contig18570.5030.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig18570.5030.1
Unique NamemRNA_H-elongata_contig18570.5030.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: D7FT75_ECTSI (Vacuolar protein 8 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FT75_ECTSI)

HSP 1 Score: 221 bits (563), Expect = 1.070e-59
Identity = 111/140 (79.29%), Postives = 119/140 (85.00%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDM 420
            EVAACLCNLSLSE+DRV +A RCVP LV +SQGGD E  RQAIG LANLAE+IDTHELIAK GGGR M  LMKHD+LD+FREASR I NLLTSFEHQA+IIE GL G+  LA STD ECQYHAALSFRKLSPNLASHR M
Sbjct: 2940 EVAACLCNLSLSEQDRVAVAARCVPALVALSQGGDLEAARQAIGTLANLAEEIDTHELIAKSGGGRVMTGLMKHDALDVFREASRAISNLLTSFEHQAVIIEQGLAGLNALAESTDPECQYHAALSFRKLSPNLASHRGM 3079
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A7S1TZK5_9STRA (Vacuolar protein 8 n=2 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TZK5_9STRA)

HSP 1 Score: 197 bits (502), Expect = 4.200e-52
Identity = 100/145 (68.97%), Postives = 114/145 (78.62%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGGG 435
            EVAACLCNLSLSE DRV IA  CV  LV +SQGGD ET RQ++G LAN+AED +TH  I K GGGR MI LM HDS+DI REASR I NLL+SF HQA IIEDG+PG+  LA S D+ECQY+AALSFRKLSPN+ASH+ +   GG
Sbjct:   25 EVAACLCNLSLSEPDRVAIAAECVEPLVFLSQGGDLETARQSVGTLANIAEDTETHGYIGKAGGGRVMIMLMDHDSIDIHREASRAISNLLSSFHHQAEIIEDGVPGLVALATSPDAECQYNAALSFRKLSPNIASHKVIIENGG 169
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A8J2SEI2_9STRA (Vacuolar protein 8 n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SEI2_9STRA)

HSP 1 Score: 158 bits (400), Expect = 3.190e-38
Identity = 76/130 (58.46%), Postives = 98/130 (75.38%), Query Frame = 1 
Query:   46 RVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGGG 435
            R+ +A+ C+P +V ++Q GDRE  RQA+G+LANLAED+ TH  +A +GG R M+ L+ HD++DI REASR I NLLTSF HQA II DGLPG+  LAL  D+ECQY+AALSFRKL+PN  +H  +   GG
Sbjct: 2979 RMDVAKVCLPAMVHLAQCGDREAARQAMGSLANLAEDMHTHTHVAAYGGARCMVALVDHDAVDIHREASRAIANLLTSFHHQATIISDGLPGLVHLALGPDAECQYNAALSFRKLTPNRDAHEGIMEHGG 3108
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A835Z6R1_9STRA (Vacuolar protein 8 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z6R1_9STRA)

HSP 1 Score: 149 bits (375), Expect = 5.850e-35
Identity = 83/160 (51.88%), Postives = 103/160 (64.38%), Query Frame = 1 
Query:    1 EVAACLCNLSL---SEEDRVTIAERCVPTLVRVSQGG-------------DRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGG 432
            E AACLCNL+L   +   R  +A+ CVP LV +++G              D ET RQAIGALANLAED  TH  IA  GG  A+  +    +LD++REASR + NLLTS E QA ++E GLPGI TLA + D+ECQYHAALS RKL+PN A+H  +C  G
Sbjct: 3149 EAAACLCNLTLGAAAPAQRAALADACVPALVALARGXXXXXXXXXXXXXXDVETARQAIGALANLAEDPATHARIAAEGGTEALAAVAAAAALDMYREASRAVCNLLTSAELQAAVVERGLPGIVTLASAPDAECQYHAALSLRKLTPNTAAHARLCAAG 3308
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A482RX95_9ARCH (Vacuolar protein 8 (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RX95_9ARCH)

HSP 1 Score: 135 bits (341), Expect = 1.370e-30
Identity = 83/188 (44.15%), Postives = 106/188 (56.38%), Query Frame = 1 
Query:    1 EVAACLCNLSLSE--EDRVTIAERCVPTLVRVSQ--------------------------GGDR---------------ETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGGG 435
            EVAA LC+LSLS+  + R+ IA +C+  LVR++                           GG+                +  RQAIGA+ANLAE++DTHE IAK    RA I L    S+DI REA+R I NLL+SF HQA IIE+G+PG+  L+ S D E  YH+ALSFRKL PN+ SH  + Y GG
Sbjct:  987 EVAATLCSLSLSKNTQHRMEIAYKCLQALVRLASVAGSEHAAISASIEKKKKEAGVEDDLGGNSGNFVPELSAHQKSLIDIARQAIGAMANLAEEVDTHEFIAKAQASRAFIALESFHSIDIQREATRAIANLLSSFRHQATIIEEGIPGLVALSYSEDLEACYHSALSFRKLGPNMKSHSIIVYAGG 1174
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A7S4T4K8_9STRA (Vacuolar protein 8 n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4T4K8_9STRA)

HSP 1 Score: 130 bits (327), Expect = 7.060e-29
Identity = 67/140 (47.86%), Postives = 91/140 (65.00%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDM 420
            E +ACLCNLSL+   R  I + C+  L R+    D +T RQA+GALAN+ E+ +TH+ + K G   + I L++H S+DI REASR I NLL S      +I  GL  + +L +++D ECQY+A+LSFRKLSPN  SH  M
Sbjct:  157 EFSACLCNLSLNVRCRADIVKTCIKELNRLLSSNDTDTVRQAMGALANITENQETHDTMQKSGTSNSTIALLQHSSMDIKREASRAIANLLGSKNQHCEVIRGGLVHLISLGVTSDPECQYNASLSFRKLSPNKDSHFTM 296
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A812KVL6_9DINO (Vacuolar protein 8 (Fragment) n=5 Tax=Sar TaxID=2698737 RepID=A0A812KVL6_9DINO)

HSP 1 Score: 120 bits (300), Expect = 2.890e-25
Identity = 63/139 (45.32%), Postives = 89/139 (64.03%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAE--RCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASH 411
            E A  + N++L +  +   A     +  LV + Q  D E  R A GALAN+AED+ TH  I+  GGGR M+ L+ H S+++ REASR + NLL SF+    +I   LPGI  LA ++D EC+YHA+L++RKL+PNLA+H
Sbjct: 3133 ETAVAIFNVTLDDVAKPAAAADPALMGGLVVLCQSPDEEVARFACGALANIAEDLATHPSISAVGGGRFMVGLIHHGSIEVHREASRALANLLASFDVHGDVIAAALPGIARLAFTSDEECRYHASLAYRKLAPNLAAH 3271
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A7S2P367_9STRA (Vacuolar protein 8 n=2 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2P367_9STRA)

HSP 1 Score: 118 bits (295), Expect = 1.250e-24
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSP 396
            E+AACLCNLS S   ++ I +RC+ TL+ +++  D E  RQ++GALANL ED+ TH + A     R++I+ + H+ +D+ REASR + NL+TS      I+E G+  +  L  S D EC YH ALS RKL+P
Sbjct: 2891 ELAACLCNLSASSH-KLIITDRCIRTLLSLAKTADEEALRQSMGALANLCEDVTTHSIFAASHALRSVIDCLCHEKIDVHREASRAVSNLVTSCCFHGAILEFGMIQLLHLCSSEDKECLYHVALSLRKLAP 3021
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A7S3JQA6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JQA6_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 1.190e-20
Identity = 48/91 (52.75%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLL 273
            EVAA LCNL+L    R+  A  C+P +V ++Q GDRE  RQA+GALANLAE  +TH  +A  GG R ++ L++H  +D+ REASR I NLL
Sbjct:   32 EVAAGLCNLTLEASARMEAARCCLPAIVNLAQCGDREVARQALGALANLAESPETHAHVAACGGSRCVVALVEHTDVDVHREASRAIANLL 122
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BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Match: A0A2R5GK93_9STRA (Vacuolar protein 8 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GK93_9STRA)

HSP 1 Score: 101 bits (252), Expect = 3.660e-19
Identity = 56/155 (36.13%), Postives = 88/155 (56.77%), Query Frame = 1 
Query:    1 EVAACLCNLSLSEEDRVTIAE--------RCVPTLVRVSQGGDRETTRQAIGALANLAEDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFE--HQAIIIEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGGG 435
            EVAA LCNLS+S+E ++ ++          C+  L+ ++Q                +AED +TH ++ +  G   +  LM+   ++++REASR I NLL S    H+  + EDGLP ++ +A S D +CQ HAAL +RKLSPN+ +H ++C   G
Sbjct: 2046 EVAAALCNLSVSDEAKLALSGCGSRRPNLECLRVLITLAQSTXXXXXXXXXXXXXXIAEDPETHPVLTEEQGIHFLSVLMRSRKVEVYREASRAISNLLGSMHVTHKTFLDEDGLPSVFRVAKSEDLQCQLHAALCYRKLSPNVRTHIEICASPG 2200
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig18570.5030.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FT75_ECTSI1.070e-5979.29Vacuolar protein 8 n=2 Tax=Ectocarpus TaxID=2879 R... [more]
A0A7S1TZK5_9STRA4.200e-5268.97Vacuolar protein 8 n=2 Tax=Phaeomonas parva TaxID=... [more]
A0A8J2SEI2_9STRA3.190e-3858.46Vacuolar protein 8 n=1 Tax=Pelagomonas calceolata ... [more]
A0A835Z6R1_9STRA5.850e-3551.88Vacuolar protein 8 n=1 Tax=Tribonema minus TaxID=3... [more]
A0A482RX95_9ARCH1.370e-3044.15Vacuolar protein 8 (Fragment) n=1 Tax=archaeon Tax... [more]
A0A7S4T4K8_9STRA7.060e-2947.86Vacuolar protein 8 n=1 Tax=Ditylum brightwellii Ta... [more]
A0A812KVL6_9DINO2.890e-2545.32Vacuolar protein 8 (Fragment) n=5 Tax=Sar TaxID=26... [more]
A0A7S2P367_9STRA1.250e-2445.45Vacuolar protein 8 n=2 Tax=Leptocylindrus danicus ... [more]
A0A7S3JQA6_9STRA1.190e-2052.75Hypothetical protein (Fragment) n=1 Tax=Aureoumbra... [more]
A0A2R5GK93_9STRA3.660e-1936.13Vacuolar protein 8 n=1 Tax=Hondaea fermentalgiana ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig18570contigH-elongata_contig18570:3085..4618 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score221.5
Seed ortholog evalue7.8e-55
Seed eggNOG ortholog2880.D7FT75
KEGG koko:K08332
EggNOG free text desc.nucleus-vacuole junction assembly
EggNOG OGsKOG0167@1,KOG0167@2759,KOG4224@1,KOG4224@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03029,ko04131
Hectar predicted targeting categoryother localisation
Exons2
Model size1305
Cds size1305
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930061.8178387-CDS-H-elongata_contig18570:3084..38491622930061.8178387-CDS-H-elongata_contig18570:3084..3849Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig18570 3085..3849 -
1691679266.0629454-CDS-H-elongata_contig18570:3084..38491691679266.0629454-CDS-H-elongata_contig18570:3084..3849Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig18570 3085..3849 -
1622930061.8339121-CDS-H-elongata_contig18570:4078..46181622930061.8339121-CDS-H-elongata_contig18570:4078..4618Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig18570 4079..4618 -
1691679266.0818064-CDS-H-elongata_contig18570:4078..46181691679266.0818064-CDS-H-elongata_contig18570:4078..4618Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig18570 4079..4618 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig18570.5030.1prot_H-elongata_contig18570.5030.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig18570 3085..4618 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig18570.5030.1

>prot_H-elongata_contig18570.5030.1 ID=prot_H-elongata_contig18570.5030.1|Name=mRNA_H-elongata_contig18570.5030.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=435bp
EVAACLCNLSLSEEDRVTIAERCVPTLVRVSQGGDRETTRQAIGALANLA
EDIDTHELIAKFGGGRAMIELMKHDSLDIFREASRVIVNLLTSFEHQAII
IEDGLPGIYTLALSTDSECQYHAALSFRKLSPNLASHRDMCYGGGLKTLF
HLLKVNDLKTRKHAATALRDLCAHADHKQQIADEGGMEALVLVALEREIE
LQILAMAGLRHLSLHDLLKPAIIEAGALRPIVRCVKWANEDLKCQVAAAL
ANLSEEFQNQIAMVEEGAVQALVTLARTENDEIQQDSSRALANLSSNEEN
HALVYRLGGLRALVGLTDSVEDVCQRYAAFGLRFLCSDPEVRVSIVQDGL
IKPFLALAQSPLIEYQRTAAATFASFSLHDENKQKMVREGCLGQILACCL
YSDLEVVRNCTFALANLADSLDLQSDVVREGGIEI
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mRNA from alignment at H-elongata_contig18570:3085..4618-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig18570.5030.1 ID=mRNA_H-elongata_contig18570.5030.1|Name=mRNA_H-elongata_contig18570.5030.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1534bp|location=Sequence derived from alignment at H-elongata_contig18570:3085..4618- (Himanthalia elongata Himel1 dioecious)
GAGGTGGCGGCCTGTTTGTGCAACTTGTCTCTAAGTGAGGAAGACCGGGT
CACGATCGCCGAGCGTTGCGTACCCACCTTGGTACGTGTGTCACAAGGCG
GAGATCGAGAAACAACAAGGCAGGCGATCGGCGCTCTCGCCAACCTTGCC
GAAGACATCGACACTCATGAGCTGATCGCCAAATTTGGTGGCGGGAGGGC
CATGATTGAGCTGATGAAACATGACTCGCTCGACATATTTCGTGAGGCAA
GCCGAGTTATCGTTAACCTGCTTACGAGTTTTGAGCACCAGGCTATTATT
ATCGAGGATGGGCTGCCAGGAATATATACCCTCGCTCTCAGCACAGATTC
CGAGTGCCAGTATCACGCCGCGCTAAGCTTCCGCAAGCTGTCGCCAAACC
TTGCCTCCCATCGAGACATGTGCTACGGTGGAGGGTTAAAGACTCTGTTC
CACCTACTGAAGGTAAATGATCTTAAGACTAGGAAGCACGCGGCGACGGC
GCTTCGGGATCTCTGCGCGCACGCTGACCACAAGCAGCAGGTCAGCGGTT
CGCCTTTTCGACCGTAGTTCACCAATGTCGCACAATGTCCCGCGCTTATC
CCTTCTTTGTGGTCGTTTCTTGGCTACTCTTGACGTGTGGCCATATTCCT
GTTTGAACCACCTGGGCAGCGTCTGTGTATAGAGTGGCGCCCATAGGTGA
TTAGCTAATGGCTAAACCTCACACCACTGCACCTATTCAATATGATTGTT
ATGTTTGTCTTGAGTCCAGATCGCAGATGAAGGCGGAATGGAAGCGCTTG
TTCTAGTGGCCTTGGAACGCGAGATCGAGCTTCAGATCCTTGCTATGGCA
GGGCTAAGGCACCTCTCTCTACACGATCTTCTCAAGCCAGCCATCATAGA
AGCAGGCGCTCTGCGGCCGATCGTGCGCTGCGTTAAGTGGGCGAACGAGG
ATCTCAAGTGCCAGGTCGCCGCAGCGCTCGCCAACCTCTCAGAGGAATTT
CAGAATCAAATCGCGATGGTGGAGGAGGGCGCCGTCCAGGCTCTCGTCAC
CCTCGCACGAACAGAAAACGACGAAATTCAACAGGACTCTTCTAGGGCGC
TCGCTAACCTTAGTAGCAACGAGGAGAACCACGCGCTTGTGTATCGACTT
GGTGGCCTTCGCGCTCTGGTGGGACTGACGGACTCTGTGGAGGACGTGTG
CCAAAGGTACGCCGCGTTCGGGCTCCGTTTCCTGTGCAGCGATCCGGAAG
TGCGCGTGAGCATCGTCCAGGACGGCCTGATCAAGCCATTCCTGGCGCTG
GCGCAGAGTCCCCTGATCGAGTATCAGCGGACTGCGGCGGCGACCTTCGC
CAGTTTTTCTCTCCACGACGAGAACAAGCAGAAGATGGTGCGAGAGGGTT
GCCTCGGGCAGATCCTGGCGTGTTGCCTCTACTCGGACCTCGAGGTGGTC
CGAAACTGTACCTTTGCTTTGGCCAATCTCGCCGACTCGCTAGATCTGCA
GAGCGACGTCGTGCGCGAGGGCGGCATTGAGATT
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Coding sequence (CDS) from alignment at H-elongata_contig18570:3085..4618-

>mRNA_H-elongata_contig18570.5030.1 ID=mRNA_H-elongata_contig18570.5030.1|Name=mRNA_H-elongata_contig18570.5030.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=2610bp|location=Sequence derived from alignment at H-elongata_contig18570:3085..4618- (Himanthalia elongata Himel1 dioecious)
GAGGTGGCGGCCTGTTTGTGCAACTTGTCTCTAAGTGAGGAAGACCGGGT
CACGATCGCCGAGCGTTGCGTACCCACCTTGGTACGTGTGTCACAAGGCG
GAGATCGAGAAACAACAAGGCAGGCGATCGGCGCTCTCGCCAACCTTGCC
GAAGACATCGACACTCATGAGCTGATCGCCAAATTTGGTGGCGGGAGGGC
CATGATTGAGCTGATGAAACATGACTCGCTCGACATATTTCGTGAGGCAA
GCCGAGTTATCGTTAACCTGCTTACGAGTTTTGAGCACCAGGCTATTATT
ATCGAGGATGGGCTGCCAGGAATATATACCCTCGCTCTCAGCACAGATTC
CGAGTGCCAGTATCACGCCGCGCTAAGCTTCCGCAAGCTGTCGCCAAACC
TTGCCTCCCATCGAGACATGTGCTACGGTGGAGGGTTAAAGACTCTGTTC
CACCTACTGAAGGTAAATGATCTTAAGACTAGGAAGCACGCGGCGACGGC
GCTTCGGGATCTCTGCGCGCACGCTGACCACAAGCAGCAGGAGGTGGCGG
CCTGTTTGTGCAACTTGTCTCTAAGTGAGGAAGACCGGGTCACGATCGCC
GAGCGTTGCGTACCCACCTTGGTACGTGTGTCACAAGGCGGAGATCGAGA
AACAACAAGGCAGGCGATCGGCGCTCTCGCCAACCTTGCCGAAGACATCG
ACACTCATGAGCTGATCGCCAAATTTGGTGGCGGGAGGGCCATGATTGAG
CTGATGAAACATGACTCGCTCGACATATTTCGTGAGGCAAGCCGAGTTAT
CGTTAACCTGCTTACGAGTTTTGAGCACCAGGCTATTATTATCGAGGATG
GGCTGCCAGGAATATATACCCTCGCTCTCAGCACAGATTCCGAGTGCCAG
TATCACGCCGCGCTAAGCTTCCGCAAGCTGTCGCCAAACCTTGCCTCCCA
TCGAGACATGTGCTACGGTGGAGGGTTAAAGACTCTGTTCCACCTACTGA
AGGTAAATGATCTTAAGACTAGGAAGCACGCGGCGACGGCGCTTCGGGAT
CTCTGCGCGCACGCTGACCACAAGCAGCAGATCGCAGATGAAGGCGGAAT
GGAAGCGCTTGTTCTAGTGGCCTTGGAACGCGAGATCGAGCTTCAGATCC
TTGCTATGGCAGGGCTAAGGCACCTCTCTCTACACGATCTTCTCAAGCCA
GCCATCATAGAAGCAGGCGCTCTGCGGCCGATCGTGCGCTGCGTTAAGTG
GGCGAACGAGGATCTCAAGTGCCAGGTCGCCGCAGCGCTCGCCAACCTCT
CAGAGGAATTTCAGAATCAAATCGCGATGGTGGAGGAGGGCGCCGTCCAG
GCTCTCGTCACCCTCGCACGAACAGAAAACGACGAAATTCAACAGGACTC
TTCTAGGGCGCTCGCTAACCTTAGTAGCAACGAGGAGAACCACGCGCTTG
TGTATCGACTTGGTGGCCTTCGCGCTCTGGTGGGACTGACGGACTCTGTG
GAGGACGTGTGCCAAAGGTACGCCGCGTTCGGGCTCCGTTTCCTGTGCAG
CGATCCGGAAGTGCGCGTGAGCATCGTCCAGGACGGCCTGATCAAGCCAT
TCCTGGCGCTGGCGCAGAGTCCCCTGATCGAGTATCAGCGGACTGCGGCG
GCGACCTTCGCCAGTTTTTCTCTCCACGACGAGAACAAGCAGAAGATGGT
GCGAGAGGGTTGCCTCGGGCAGATCCTGGCGTGTTGCCTCTACTCGGACC
TCGAGGTGGTCCGAAACTGTACCTTTGCTTTGGCCAATCTCGCCGACTCG
CTAGATCTGCAGAGCGACGTCGTGCGCGAGGGCGGCATTGAGATTATCGC
AGATGAAGGCGGAATGGAAGCGCTTGTTCTAGTGGCCTTGGAACGCGAGA
TCGAGCTTCAGATCCTTGCTATGGCAGGGCTAAGGCACCTCTCTCTACAC
GATCTTCTCAAGCCAGCCATCATAGAAGCAGGCGCTCTGCGGCCGATCGT
GCGCTGCGTTAAGTGGGCGAACGAGGATCTCAAGTGCCAGGTCGCCGCAG
CGCTCGCCAACCTCTCAGAGGAATTTCAGAATCAAATCGCGATGGTGGAG
GAGGGCGCCGTCCAGGCTCTCGTCACCCTCGCACGAACAGAAAACGACGA
AATTCAACAGGACTCTTCTAGGGCGCTCGCTAACCTTAGTAGCAACGAGG
AGAACCACGCGCTTGTGTATCGACTTGGTGGCCTTCGCGCTCTGGTGGGA
CTGACGGACTCTGTGGAGGACGTGTGCCAAAGGTACGCCGCGTTCGGGCT
CCGTTTCCTGTGCAGCGATCCGGAAGTGCGCGTGAGCATCGTCCAGGACG
GCCTGATCAAGCCATTCCTGGCGCTGGCGCAGAGTCCCCTGATCGAGTAT
CAGCGGACTGCGGCGGCGACCTTCGCCAGTTTTTCTCTCCACGACGAGAA
CAAGCAGAAGATGGTGCGAGAGGGTTGCCTCGGGCAGATCCTGGCGTGTT
GCCTCTACTCGGACCTCGAGGTGGTCCGAAACTGTACCTTTGCTTTGGCC
AATCTCGCCGACTCGCTAGATCTGCAGAGCGACGTCGTGCGCGAGGGCGG
CATTGAGATT
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