mRNA_H-elongata_contig169823.4333.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig169823.4333.1
Unique NamemRNA_H-elongata_contig169823.4333.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: D7G1I0_ECTSI (RNA helicase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1I0_ECTSI)

HSP 1 Score: 199 bits (506), Expect = 3.140e-58
Identity = 101/104 (97.12%), Postives = 101/104 (97.12%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQA 312
            RSMVSAENVREQLGRLM RLELRMVSTEFT RDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVW MFEEFVLTSKNYIRTVTGVQA
Sbjct:  568 RSMVSAENVREQLGRLMGRLELRMVSTEFTARDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWCMFEEFVLTSKNYIRTVTGVQA 671
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A6H5L043_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L043_9PHAE)

HSP 1 Score: 174 bits (442), Expect = 4.780e-49
Identity = 87/91 (95.60%), Postives = 87/91 (95.60%), Query Frame = 1 
Query:   40 GRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQA 312
             RLM RLELRMVSTEFT RDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVW MFEEFVLTSKNYIRTVTGVQA
Sbjct:  591 SRLMGRLELRMVSTEFTARDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWCMFEEFVLTSKNYIRTVTGVQA 681
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A836CBU4_9STRA (RNA helicase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CBU4_9STRA)

HSP 1 Score: 164 bits (415), Expect = 2.870e-45
Identity = 82/103 (79.61%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RSM SA+NVREQL RLM RL L + STEFT+RDYYTNIKKAL AGMFMQVAH ERS HYLTVKDNQ+V IHPSS LD+KP W +FEEFVLTSKN+IRTVT VQ
Sbjct:  570 RSMGSADNVREQLSRLMQRLNLPLKSTEFTNRDYYTNIKKALCAGMFMQVAHRERSDHYLTVKDNQIVGIHPSSALDSKPEWVIFEEFVLTSKNFIRTVTAVQ 672
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A183QYA0_9TREM (OB_NTP_bind domain-containing protein n=2 Tax=Schistosoma TaxID=6181 RepID=A0A183QYA0_9TREM)

HSP 1 Score: 150 bits (380), Expect = 1.930e-44
Identity = 71/103 (68.93%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS+ SA+NVR QL R+M R  LR  ST+FT RDYY NI+KAL +G FMQVAHLER+GHYLTVKDNQVV +HPS+V+D+KP W ++ EFVLT+KNYIRTVT V+
Sbjct:   20 RSLKSADNVRVQLSRIMDRFSLRRSSTDFTSRDYYINIRKALVSGFFMQVAHLERTGHYLTVKDNQVVQLHPSTVMDHKPEWVLYNEFVLTTKNYIRTVTEVK 122
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A0M3KHY3_ANISI (OB_NTP_bind domain-containing protein n=2 Tax=Anisakis simplex TaxID=6269 RepID=A0A0M3KHY3_ANISI)

HSP 1 Score: 149 bits (377), Expect = 3.950e-44
Identity = 69/104 (66.35%), Postives = 88/104 (84.62%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQA 312
            RS+ +A+NVR QL R+M +  L+ VST+F  +DYY NI+KAL +G FMQVAHLERSGHY+TVKDNQ+V +HPS+VLD+KP WA++ EFVLT+KNYIRTVT V+A
Sbjct:   21 RSLKNADNVRTQLARIMDKYNLKRVSTDFKSKDYYINIRKALVSGFFMQVAHLERSGHYITVKDNQLVQLHPSTVLDHKPEWALYNEFVLTTKNYIRTVTDVKA 124
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: UPI00096B1D29 (pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15-like n=1 Tax=Amphimedon queenslandica TaxID=400682 RepID=UPI00096B1D29)

HSP 1 Score: 152 bits (383), Expect = 1.080e-43
Identity = 70/103 (67.96%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS+ SA+NVR+QL R+M R  L+  ST+F+ +DYYTNI+KAL AG FMQVAHLERSGHYLTVKDNQVV +HPS+ LD+KP W ++ EFVLTSKNYIRT T ++
Sbjct:  130 RSLKSADNVRDQLSRIMDRFNLKRTSTDFSSKDYYTNIRKALVAGFFMQVAHLERSGHYLTVKDNQVVQLHPSTCLDHKPEWVLYNEFVLTSKNYIRTCTDIK 232
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A1A9V383_GLOAU (OB_NTP_bind domain-containing protein n=4 Tax=Glossina TaxID=7393 RepID=A0A1A9V383_GLOAU)

HSP 1 Score: 149 bits (377), Expect = 1.260e-43
Identity = 69/103 (66.99%), Postives = 85/103 (82.52%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS+ SA+NVREQL R+M R  L+  STEFT +DYY NI+KAL  G FMQVAHLER+GHYLT+KDNQ+V +HPS+ LD+KP W ++ EFVLT+KNYIRTVT V+
Sbjct:   60 RSLKSADNVREQLARIMDRFNLKRSSTEFTSKDYYVNIRKALVQGFFMQVAHLERTGHYLTIKDNQIVQLHPSTCLDHKPDWVIYNEFVLTTKNYIRTVTDVK 162
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A2D4LTP4_9SAUR (OB_NTP_bind domain-containing protein n=2 Tax=Amniota TaxID=32524 RepID=A0A2D4LTP4_9SAUR)

HSP 1 Score: 148 bits (374), Expect = 1.390e-43
Identity = 69/103 (66.99%), Postives = 85/103 (82.52%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS++SA+NVR+QL R+M R  L   ST+FT RDYY NI+KAL  G FMQVAHLER+GHYLTVKDNQVV +HPS+VLD+KP W ++ EFVLT+KNYIRT T ++
Sbjct:   29 RSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIK 131
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BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: A0A293MUQ8_ORNER (OB_NTP_bind domain-containing protein (Fragment) n=1 Tax=Ornithodoros erraticus TaxID=265619 RepID=A0A293MUQ8_ORNER)

HSP 1 Score: 149 bits (377), Expect = 1.830e-43
Identity = 68/103 (66.02%), Postives = 85/103 (82.52%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS+ SA+NVR+QL R+M R  LR  STEFT +DYY NI+K L +G FMQVAHLER+GHYLT+KDNQVV +HPS+ LD+KP W ++ EFVLT+KNYIRTVT ++
Sbjct:   73 RSLKSADNVRQQLSRIMDRFNLRRTSTEFTSKDYYINIRKTLVSGFFMQVAHLERTGHYLTIKDNQVVQLHPSTCLDHKPEWVVYNEFVLTTKNYIRTVTDIK 175
[Close]
BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Match: UPI0014035809 (pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15-like n=2 Tax=Vertebrata TaxID=7742 RepID=UPI0014035809)

HSP 1 Score: 152 bits (384), Expect = 1.860e-43
Identity = 71/103 (68.93%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:    1 RSMVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAHLERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGVQ 309
            RS++SA+NVR+QLGR+M R  L   STEFT RDYY+NI++AL +G FMQVAHLER+GHYLTVKDNQVV +HPS+ LD+KP W ++ EFVLT+KNYIRTVT V+
Sbjct:  166 RSLMSADNVRQQLGRIMDRFALPRRSTEFTSRDYYSNIRRALVSGFFMQVAHLERTGHYLTVKDNQVVQLHPSTCLDHKPEWVLYNEFVLTTKNYIRTVTDVK 268
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig169823.4333.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G1I0_ECTSI3.140e-5897.12RNA helicase n=1 Tax=Ectocarpus siliculosus TaxID=... [more]
A0A6H5L043_9PHAE4.780e-4995.60Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836CBU4_9STRA2.870e-4579.61RNA helicase n=1 Tax=Tribonema minus TaxID=303371 ... [more]
A0A183QYA0_9TREM1.930e-4468.93OB_NTP_bind domain-containing protein n=2 Tax=Schi... [more]
A0A0M3KHY3_ANISI3.950e-4466.35OB_NTP_bind domain-containing protein n=2 Tax=Anis... [more]
UPI00096B1D291.080e-4367.96pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]
A0A1A9V383_GLOAU1.260e-4366.99OB_NTP_bind domain-containing protein n=4 Tax=Glos... [more]
A0A2D4LTP4_9SAUR1.390e-4366.99OB_NTP_bind domain-containing protein n=2 Tax=Amni... [more]
A0A293MUQ8_ORNER1.830e-4366.02OB_NTP_bind domain-containing protein (Fragment) n... [more]
UPI00140358091.860e-4368.93pre-mRNA-splicing factor ATP-dependent RNA helicas... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig169823contigH-elongata_contig169823:97..897 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score198.0
Seed ortholog evalue2.2e-48
Seed eggNOG ortholog2880.D7G1I0
Preferred nameDHX15
KEGG koko:K04615,ko:K12820,ko:K14305
KEGG TC1.l.1,9.A.14.15.1
KEGG Pathwayko03013,ko03040,ko04024,ko04080,ko04727,ko04742,ko04915,ko05032,map03013,map03040,map04024,map04080,map04727,map04742,map04915,map05032
KEGG ModuleM00427
GOsGO:0000166,GO:0000375,GO:0000377,GO:0000390,GO:0000398,GO:0000460,GO:0000462,GO:0000463,GO:0000466,GO:0000470,GO:0001932,GO:0001934,GO:0003674,GO:0003676,GO:0003723,GO:0003724,GO:0003725,GO:0003729,GO:0003824,GO:0004004,GO:0004386,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005681,GO:0005684,GO:0005689,GO:0005730,GO:0005737,GO:0005739,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0008026,GO:0008144,GO:0008150,GO:0008152,GO:0008186,GO:0008380,GO:0009507,GO:0009526,GO:0009536,GO:0009893,GO:0009941,GO:0009966,GO:0009967,GO:0009987,GO:0010467,GO:0010562,GO:0010604,GO:0010646,GO:0010647,GO:0016020,GO:0016043,GO:0016070,GO:0016071,GO:0016072,GO:0016462,GO:0016604,GO:0016607,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0019220,GO:0019222,GO:0022411,GO:0022613,GO:0023051,GO:0023056,GO:0030490,GO:0030554,GO:0030684,GO:0030686,GO:0031323,GO:0031325,GO:0031399,GO:0031401,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032268,GO:0032270,GO:0032553,GO:0032555,GO:0032559,GO:0032872,GO:0032874,GO:0032984,GO:0032988,GO:0032991,GO:0034470,GO:0034641,GO:0034660,GO:0035639,GO:0036094,GO:0042254,GO:0042273,GO:0042274,GO:0042325,GO:0042327,GO:0042623,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043408,GO:0043410,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0045937,GO:0046483,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051174,GO:0051246,GO:0051247,GO:0060255,GO:0065007,GO:0070013,GO:0070035,GO:0070302,GO:0070304,GO:0071014,GO:0071704,GO:0071826,GO:0071840,GO:0080090,GO:0080134,GO:0080135,GO:0090304,GO:0097159,GO:0097367,GO:0140098,GO:1900744,GO:1900745,GO:1901265,GO:1901360,GO:1901363,GO:1902531,GO:1902533,GO:1990904
EggNOG free text desc.helicase activity
EggNOG OGsCOG1643@1,KOG0925@2759
EC3.6.4.13
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03009,ko03019,ko03036,ko03041,ko04030
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionRNA helicase family protein
Ec32 orthologEc-07_001080.1
Exons2
Model size312
Cds size306
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig169823.4333.1prot_H-elongata_contig169823.4333.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig169823 97..891 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929990.847893-CDS-H-elongata_contig169823:96..2611622929990.847893-CDS-H-elongata_contig169823:96..261Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169823 97..261 -
1691679237.2647407-CDS-H-elongata_contig169823:96..2611691679237.2647407-CDS-H-elongata_contig169823:96..261Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169823 97..261 -
1622929990.867807-CDS-H-elongata_contig169823:750..8911622929990.867807-CDS-H-elongata_contig169823:750..891Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169823 751..891 -
1691679237.2806702-CDS-H-elongata_contig169823:750..8911691679237.2806702-CDS-H-elongata_contig169823:750..891Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169823 751..891 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929990.8793528-UTR-H-elongata_contig169823:891..8971622929990.8793528-UTR-H-elongata_contig169823:891..897Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig169823 892..897 -
1691679237.2895994-UTR-H-elongata_contig169823:891..8971691679237.2895994-UTR-H-elongata_contig169823:891..897Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig169823 892..897 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig169823.4333.1

>prot_H-elongata_contig169823.4333.1 ID=prot_H-elongata_contig169823.4333.1|Name=mRNA_H-elongata_contig169823.4333.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=102bp
MVSAENVREQLGRLMSRLELRMVSTEFTDRDYYTNIKKALTAGMFMQVAH
LERSGHYLTVKDNQVVSIHPSSVLDNKPVWAMFEEFVLTSKNYIRTVTGV
QA
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mRNA from alignment at H-elongata_contig169823:97..897-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig169823.4333.1 ID=mRNA_H-elongata_contig169823.4333.1|Name=mRNA_H-elongata_contig169823.4333.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=801bp|location=Sequence derived from alignment at H-elongata_contig169823:97..897- (Himanthalia elongata Himel1 dioecious)
AGATCAATGGTGTCAGCAGAGAACGTGAGGGAGCAGCTGGGGAGGCTGAT
GTCACGGCTGGAGCTGCGCATGGTGTCGACGGAGTTCACCGACAGAGACT
ACTACACGAACATAAAAAAGGCTCTGACTGCGGGCATGTTTATGCAGGTG
GCAAAACATACACGCTACGTTTGCTTGCGTGTTTGTGTTTGAAAGGAGAA
ATATATGAGGTTTGCGGGGGGGGAGGGTCTATCTCGTTCGAGCTATATTG
CTGCTATCCTCCCATTTGTTTAAGATTGTTTCACTTTGCAGTAGTAGGGT
TTATTTGAGGCCATTCCATTTGATCCTCCCGTGCACGGCGCGAGGGGCCG
GGACGGTATGGGATGGGACACGACGGGACGTTCTTGGATCGAGCGGCCAT
CACACTACATACTGCTGTTTTTGCCGCTCTCGAACCTAGAATGTTTTGCC
TCTCCGTTCGACCCCGACTGTGCGCATCTGCTGCATGGTGTGTGTGTGTG
TGTGTGTGTGTCGTTTCGTTCTAGTCCTGTGAATGCGAGGCGCTCCGCTG
TTGCTGTTTCTATAACCGCTCTCTCTCTCTAGTCCTTCCTCCCCCCCAAA
CCCTCCTTTGACCCCTCCCCACCCTCCCGTCGAAAGGTGGCACACCTGGA
GCGATCGGGACACTACCTGACCGTCAAAGATAACCAGGTTGTGTCGATCC
ATCCTTCATCGGTGCTGGACAACAAGCCTGTTTGGGCCATGTTCGAGGAG
TTCGTGCTCACCTCAAAGAACTACATCCGTACTGTCACGGGCGTGCAGGC
G
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Coding sequence (CDS) from alignment at H-elongata_contig169823:97..897-

>mRNA_H-elongata_contig169823.4333.1 ID=mRNA_H-elongata_contig169823.4333.1|Name=mRNA_H-elongata_contig169823.4333.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=612bp|location=Sequence derived from alignment at H-elongata_contig169823:97..897- (Himanthalia elongata Himel1 dioecious)
ATGGTGTCAGCAGAGAACGTGAGGGAGCAGCTGGGGAGGCTGATGTCACG
GCTGGAGCTGCGCATGGTGTCGACGGAGTTCACCGACAGAGACTACTACA
CGAACATAAAAAAGGCTCTGACTGCGGGCATGTTTATGCAGATGGTGTCA
GCAGAGAACGTGAGGGAGCAGCTGGGGAGGCTGATGTCACGGCTGGAGCT
GCGCATGGTGTCGACGGAGTTCACCGACAGAGACTACTACACGAACATAA
AAAAGGCTCTGACTGCGGGCATGTTTATGCAGGTGGCACACCTGGAGCGA
TCGGGACACTACCTGACCGTCAAAGATAACCAGGTTGTGTCGATCCATCC
TTCATCGGTGCTGGACAACAAGCCTGTTTGGGCCATGTTCGAGGAGTTCG
TGCTCACCTCAAAGAACTACATCCGTACTGTCACGGGCGTGCAGGCGGTG
GCACACCTGGAGCGATCGGGACACTACCTGACCGTCAAAGATAACCAGGT
TGTGTCGATCCATCCTTCATCGGTGCTGGACAACAAGCCTGTTTGGGCCA
TGTTCGAGGAGTTCGTGCTCACCTCAAAGAACTACATCCGTACTGTCACG
GGCGTGCAGGCG
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