mRNA_H-elongata_contig16743.4206.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig16743.4206.1
Unique NamemRNA_H-elongata_contig16743.4206.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: D8LES4_ECTSI (CCT-theta n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LES4_ECTSI)

HSP 1 Score: 172 bits (435), Expect = 3.800e-49
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            VSDMCMHFIEKYEMMV+KV SKWELRR+CNAVHATALVRMGAPTPDEMG C+LVEVRE+GLRKVTVFSQ+DDDSRIATIVL AST SLLNDLE
Sbjct:  302 VSDMCMHFIEKYEMMVIKVLSKWELRRICNAVHATALVRMGAPTPDEMGDCALVEVREVGLRKVTVFSQDDDDSRIATIVLRASTQSLLNDLE 394
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A6H5KSW1_9PHAE (CCT-theta n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSW1_9PHAE)

HSP 1 Score: 158 bits (399), Expect = 4.850e-45
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSL 264
            VSDMCMHFIEKYEMMV+KV SKWELRR+CNAVHATALVRMGAPTPDEMG C+LVEVRE+GLRKVTVFSQ+DDDSRIATIVL A + SL
Sbjct:  302 VSDMCMHFIEKYEMMVIKVLSKWELRRICNAVHATALVRMGAPTPDEMGDCALVEVREVGLRKVTVFSQDDDDSRIATIVLRARSVSL 389
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: K3WHS8_GLOUD (CCT-theta n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WHS8_GLOUD)

HSP 1 Score: 136 bits (342), Expect = 9.730e-36
Identity = 61/93 (65.59%), Postives = 83/93 (89.25%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKY+++ VK+QSKWELRR+C AV+A ALVR+GAPTPDEMG C  V V+EIG +KVTVF+QED+D++I+TI+L ASTD++LND+E
Sbjct:  302 ISEMALHFLEKYKLLAVKIQSKWELRRLCRAVNANALVRLGAPTPDEMGLCDSVTVKEIGGKKVTVFNQEDEDAKISTIILRASTDNVLNDIE 394
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A425BWK5_9STRA (Uncharacterized protein n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A425BWK5_9STRA)

HSP 1 Score: 134 bits (337), Expect = 1.110e-35
Identity = 61/93 (65.59%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M MHF+EKY +M +K+QSKWELRR+C AV+A ALVR+GAPTPDEMG C  V V+EIG +KVTVF Q+ +D++I+TIVL ASTD++LND+E
Sbjct:  180 ISEMAMHFLEKYNLMAIKIQSKWELRRLCRAVNANALVRLGAPTPDEMGFCDSVTVKEIGGKKVTVFQQDQEDAKISTIVLRASTDNVLNDIE 272
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A485KST6_9STRA (CCT-theta n=6 Tax=Saprolegniaceae TaxID=4764 RepID=A0A485KST6_9STRA)

HSP 1 Score: 134 bits (337), Expect = 4.950e-35
Identity = 58/93 (62.37%), Postives = 85/93 (91.40%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKYE++ +++QSKWELRR+C AV+ATALVR+GAPTP+EMG C  V+V+EIG +K+TVFSQ+++D++++TI+L ASTD++LNDLE
Sbjct:  302 ISEMALHFLEKYELLSIRIQSKWELRRLCRAVNATALVRLGAPTPEEMGFCDHVQVKEIGGKKITVFSQDEEDAKMSTIILRASTDNVLNDLE 394
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A067BSY5_SAPPC (Uncharacterized protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067BSY5_SAPPC)

HSP 1 Score: 129 bits (324), Expect = 5.180e-35
Identity = 55/93 (59.14%), Postives = 82/93 (88.17%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKY+++ +++QSKWELRR+C AV+ATALVR+GAPTP+EMG C  V V EIG +K+T+F Q+++D++++TI+L ASTD++LNDLE
Sbjct:   32 ISEMALHFLEKYKLLSIRIQSKWELRRLCRAVNATALVRLGAPTPEEMGFCDFVGVHEIGGKKITIFRQDEEDAKMSTIILRASTDNVLNDLE 124
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: H3G986_PHYRM (CCT-theta n=27 Tax=Peronosporaceae TaxID=4777 RepID=H3G986_PHYRM)

HSP 1 Score: 133 bits (334), Expect = 1.320e-34
Identity = 60/93 (64.52%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKY +M +K+QSKWELRR+C AV+A A+VR+GAPTPDEMG C  V V+EIG +KVTVF QE +D++I+TIVL ASTD++LND+E
Sbjct:  302 ISEMSLHFLEKYNLMAIKIQSKWELRRLCRAVNANAMVRLGAPTPDEMGFCDSVTVKEIGGKKVTVFEQEQEDAKISTIVLRASTDNVLNDIE 394
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: M4B3A9_HYAAE (CCT-theta n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4B3A9_HYAAE)

HSP 1 Score: 132 bits (333), Expect = 2.200e-34
Identity = 60/93 (64.52%), Postives = 80/93 (86.02%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKY +M +K+QSKWELRR+C AV+A ALVR+GAPTPDEMG C  V V+EIG +KVTVF Q+ +D++I+TIVL ASTD++LND+E
Sbjct:  302 ISEMALHFLEKYNLMAIKIQSKWELRRLCRAVNANALVRLGAPTPDEMGFCDSVMVKEIGGKKVTVFQQDQEDAKISTIVLRASTDNVLNDIE 394
[Close]
BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A2D4C5B0_PYTIN (CCT-theta n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C5B0_PYTIN)

HSP 1 Score: 132 bits (331), Expect = 8.230e-34
Identity = 59/93 (63.44%), Postives = 81/93 (87.10%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M MHF+EKY++M +K+QSKWELRR+C AV+A ALVR+GAPTPDEMG C  V V+EIG +KVT+F QE +D++++TIVL AST+++LND+E
Sbjct:  482 ISEMAMHFLEKYQLMAIKIQSKWELRRLCRAVNANALVRLGAPTPDEMGFCDSVMVKEIGGKKVTIFRQEKEDAKMSTIVLRASTENVLNDIE 574
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BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Match: A0A1W0AAN5_9STRA (CCT-theta (Fragment) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0AAN5_9STRA)

HSP 1 Score: 132 bits (331), Expect = 9.460e-34
Identity = 57/93 (61.29%), Postives = 84/93 (90.32%), Query Frame = 1 
Query:    1 VSDMCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSLVEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE 279
            +S+M +HF+EKY+++ +++QSKWELRR+C AV+ATALVR+GAPTP+EMG C  V V+E+G +KVT+FSQE++D++++TI+L ASTD++LNDLE
Sbjct:  316 ISEMALHFLEKYKLLSIRIQSKWELRRLCRAVNATALVRLGAPTPEEMGFCDHVAVQEVGGKKVTIFSQEEEDAKMSTIILRASTDNVLNDLE 408
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig16743.4206.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LES4_ECTSI3.800e-4990.32CCT-theta n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
A0A6H5KSW1_9PHAE4.850e-4587.50CCT-theta n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxI... [more]
K3WHS8_GLOUD9.730e-3665.59CCT-theta n=1 Tax=Globisporangium ultimum (strain ... [more]
A0A425BWK5_9STRA1.110e-3565.59Uncharacterized protein n=1 Tax=Peronospora effusa... [more]
A0A485KST6_9STRA4.950e-3562.37CCT-theta n=6 Tax=Saprolegniaceae TaxID=4764 RepID... [more]
A0A067BSY5_SAPPC5.180e-3559.14Uncharacterized protein n=1 Tax=Saprolegnia parasi... [more]
H3G986_PHYRM1.320e-3464.52CCT-theta n=27 Tax=Peronosporaceae TaxID=4777 RepI... [more]
M4B3A9_HYAAE2.200e-3464.52CCT-theta n=1 Tax=Hyaloperonospora arabidopsidis (... [more]
A0A2D4C5B0_PYTIN8.230e-3463.44CCT-theta n=2 Tax=Pythium insidiosum TaxID=114742 ... [more]
A0A1W0AAN5_9STRA9.460e-3461.29CCT-theta (Fragment) n=1 Tax=Thraustotheca clavata... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig16743contigH-elongata_contig16743:5541..6788 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score166.0
Seed ortholog evalue8.1e-39
Seed eggNOG ortholog2880.D8LES4
Preferred nameCCT8
KEGG koko:K09500
GOsGO:0000003,GO:0000323,GO:0001775,GO:0002199,GO:0002252,GO:0002263,GO:0002274,GO:0002275,GO:0002283,GO:0002366,GO:0002376,GO:0002443,GO:0002444,GO:0002446,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005764,GO:0005766,GO:0005773,GO:0005775,GO:0005813,GO:0005815,GO:0005829,GO:0005832,GO:0005856,GO:0005874,GO:0005911,GO:0006457,GO:0006458,GO:0006810,GO:0006887,GO:0006955,GO:0007338,GO:0007339,GO:0008037,GO:0008150,GO:0009506,GO:0009566,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0009988,GO:0010556,GO:0010557,GO:0010604,GO:0010638,GO:0012505,GO:0015630,GO:0016020,GO:0016043,GO:0016192,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019219,GO:0019222,GO:0019953,GO:0022414,GO:0022607,GO:0030054,GO:0030141,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031410,GO:0031647,GO:0031974,GO:0031981,GO:0031982,GO:0031983,GO:0032204,GO:0032206,GO:0032210,GO:0032212,GO:0032879,GO:0032880,GO:0032940,GO:0032991,GO:0033043,GO:0033044,GO:0034774,GO:0035036,GO:0035578,GO:0036230,GO:0042119,GO:0042582,GO:0042623,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043299,GO:0043312,GO:0043933,GO:0044085,GO:0044183,GO:0044297,GO:0044422,GO:0044424,GO:0044428,GO:0044430,GO:0044433,GO:0044437,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0044703,GO:0045055,GO:0045111,GO:0045321,GO:0045935,GO:0046903,GO:0046931,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051052,GO:0051054,GO:0051082,GO:0051128,GO:0051130,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051704,GO:0055044,GO:0060205,GO:0060255,GO:0060341,GO:0061077,GO:0065003,GO:0065007,GO:0065008,GO:0070013,GO:0070201,GO:0070202,GO:0070203,GO:0071840,GO:0080090,GO:0097708,GO:0099080,GO:0099081,GO:0099503,GO:0099512,GO:0099513,GO:0101002,GO:0101031,GO:1901998,GO:1903827,GO:1903829,GO:1904356,GO:1904358,GO:1904813,GO:1904814,GO:1904816,GO:1904851,GO:1904951,GO:2000112,GO:2000278,GO:2000573,GO:2001252
EggNOG free text desc.assists the folding of proteins upon ATP hydrolysis
EggNOG OGsKOG0362@1,KOG0362@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko03110
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionTCP-1-like chaperonin intermediate domain
Ec32 orthologEc-21_005380.1
Exons2
Model size279
Cds size270
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig16743.4206.1prot_H-elongata_contig16743.4206.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig16743 5541..6779 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929977.1568425-CDS-H-elongata_contig16743:5540..55681622929977.1568425-CDS-H-elongata_contig16743:5540..5568Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig16743 5541..5568 -
1691679231.8027034-CDS-H-elongata_contig16743:5540..55681691679231.8027034-CDS-H-elongata_contig16743:5540..5568Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig16743 5541..5568 -
1622929977.1686418-CDS-H-elongata_contig16743:6537..67791622929977.1686418-CDS-H-elongata_contig16743:6537..6779Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig16743 6538..6779 -
1691679231.8121743-CDS-H-elongata_contig16743:6537..67791691679231.8121743-CDS-H-elongata_contig16743:6537..6779Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig16743 6538..6779 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929977.1869519-UTR-H-elongata_contig16743:6779..67881622929977.1869519-UTR-H-elongata_contig16743:6779..6788Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig16743 6780..6788 -
1691679231.8214085-UTR-H-elongata_contig16743:6779..67881691679231.8214085-UTR-H-elongata_contig16743:6779..6788Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig16743 6780..6788 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig16743.4206.1

>prot_H-elongata_contig16743.4206.1 ID=prot_H-elongata_contig16743.4206.1|Name=mRNA_H-elongata_contig16743.4206.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=90bp
MCMHFIEKYEMMVVKVQSKWELRRVCNAVHATALVRMGAPTPDEMGSCSL
VEVREIGLRKVTVFSQEDDDSRIATIVLGASTDSLLNDLE
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mRNA from alignment at H-elongata_contig16743:5541..6788-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig16743.4206.1 ID=mRNA_H-elongata_contig16743.4206.1|Name=mRNA_H-elongata_contig16743.4206.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1248bp|location=Sequence derived from alignment at H-elongata_contig16743:5541..6788- (Himanthalia elongata Himel1 dioecious)
GTCAGCGACATGTGCATGCATTTTATTGAGAAGTATGAGATGATGGTGGT
AAAGGTGCAGAGCAAGTGGGAACTGCGGCGCGTGTGCAACGCGGTGCACG
CCACCGCGCTCGTCCGCATGGGTGCGCCTACGCCCGACGAGATGGGGAGC
TGCTCCCTCGTCGAGGTTCGGGAGATCGGGCTCCGCAAGGTGACGGTGTT
CAGCCAAGAAGACGACGACTCTCGCATCGCCACAATCGTCCTCGGGGCCA
GGTTAGCCCGTCCGGGGGGGGGGGGGTTACAGGGACTCGCGCCACCTTGC
TTCATGAGTTTGCAACCGGTTGCCCCAGTGGTTCAGTTGGTAACCTCGAA
ATCTTCTGACGTTGGGACGTGAGTTCGAATCTCGGCGCAGTGACACGACC
TCGAATTTTTTCTCATTATCATGCCCAACAAGTCGAGAACGATTATTTCG
TGGGGACACAAAATTTTACATACTATATACAGTATACTATTCACGGTCGA
TGAGGGAAAGGGACTATGACGGCTAAATCCTTCTCGCGATAAAAAAAAAT
ATAAGGCAAGCATCGCATAAGGATTTTTGGGAAAATATTTTGTGACCTTC
CTCCTGTGTGATTGTTGGATGAGAGAGGGTTGGAAACCTGTATCTTGCTT
TGATCTCGAGGATGGAGAGAAAGTTGACGGGTGGCAGTCCCTTGTTTTTG
GGAGAAAGGTTTCTGACTTCAGATGAAACATTCTCCGACGATTCTCTTGA
TGTATCTTTGTGATGCGTTTTGGTTAGAAATCTGGGAGGGGAAATGAATC
GTTGTACAGCGGGAGAAGGAATCGCGGCTCGGGTAGGTGTGAAGACCGTG
TCCACGTATTTTGATTAATAAAGTGTAGCGGCAGAATGGTCGTTAAATGA
AAAAAAGAGAGATATATGTGGGTATCTACGTCTAAACTCGTAAAAATTGA
TGTTTTTGGTGGGAGATAATGTTTGAGGCATGGGCTATAAATAAAGCTAC
TGGTGGGCAAGAAGTAGTCTAAGAGGGCGTAAAACGGATGTAGACTGGAT
ATGACCAAAACATGCTTGCGGCCATCGGCGATCGATCACGTAGGAACCAT
GAATGCTCCGTCCTTCCGTGTAAGCGCAAACCATGAGTACATACCTCCCC
GCCCCCCTCTATCTCTCTATCTTTCTCGCTTGCTCACTCTCTCCCTCAAA
CGTGCATTCTCAACAAACAGCACCGACTCTCTCCTGAATGACCTCGAG
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Coding sequence (CDS) from alignment at H-elongata_contig16743:5541..6788-

>mRNA_H-elongata_contig16743.4206.1 ID=mRNA_H-elongata_contig16743.4206.1|Name=mRNA_H-elongata_contig16743.4206.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=540bp|location=Sequence derived from alignment at H-elongata_contig16743:5541..6788- (Himanthalia elongata Himel1 dioecious)
ATGTGCATGCATTTTATTGAGAAGTATGAGATGATGGTGGTAAAGGTGCA
GAGCAAGTGGGAACTGCGGCGCGTGTGCAACGCGGTGCACGCCACCGCGC
TCGTCCGCATGGGTGCGCCTACGCCCGACGAGATGGGGAGCTGCTCCCTC
GTCGAGGTTCGGGAGATCGGGCTCCGCAAGGTGACGGTGTTCAGCCAAGA
AGACGACGACTCTCGCATCGCCACAATCGTCCTCGGGGCCAGATGTGCAT
GCATTTTATTGAGAAGTATGAGATGATGGTGGTAAAGGTGCAGAGCAAGT
GGGAACTGCGGCGCGTGTGCAACGCGGTGCACGCCACCGCGCTCGTCCGC
ATGGGTGCGCCTACGCCCGACGAGATGGGGAGCTGCTCCCTCGTCGAGGT
TCGGGAGATCGGGCTCCGCAAGGTGACGGTGTTCAGCCAAGAAGACGACG
ACTCTCGCATCGCCACAATCGTCCTCGGGGCCAGCACCGACTCTCTCCTG
AATGACCTCGAGCACCGACTCTCTCCTGAATGACCTCGAG
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