mRNA_H-elongata_contig160830.3895.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig160830.3895.1
Unique NamemRNA_H-elongata_contig160830.3895.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: D7FUS1_ECTSI (Nitrate/nitrite transporter n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FUS1_ECTSI)

HSP 1 Score: 244 bits (622), Expect = 1.160e-76
Identity = 122/156 (78.21%), Postives = 139/156 (89.10%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGE-YRRASI-FTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            W++FAQYAACFGVELTVNNVAA YF+DEFGLST++AG+VASLFGLMN+FARS+GG WS   YR+     TGMRGRF AQW+AL+WE  FL++FT MN LG AI +LILFS+GVQM+EGTSYGIVPY+VPDATGAVSGIVGAGGNFGAVMWGLIFRF
Sbjct:  254 WIMFAQYAACFGVELTVNNVAANYFSDEFGLSTSKAGMVASLFGLMNLFARSVGGIWSDFLYRKFGAGVTGMRGRFFAQWSALVWEGCFLFLFTYMNTLGAAIPILILFSVGVQMSEGTSYGIVPYIVPDATGAVSGIVGAGGNFGAVMWGLIFRF 409
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: D7FUR8_ECTSI (Nitrate high affinity transporter n=4 Tax=Ectocarpus TaxID=2879 RepID=D7FUR8_ECTSI)

HSP 1 Score: 243 bits (621), Expect = 1.640e-76
Identity = 124/157 (78.98%), Postives = 137/157 (87.26%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRRASI---FTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            WL+F QYAACFGVELTVNNVAA YF+DEF LST++AG+VASLFGLMN+FARSLGG WS ++  A      TGMRGRF AQWTAL+WE  FL++FT MN LG AI VLILFS+GVQMAEGTSYGIVPY+VPDATGAVSGIVGAGGNFGAVMWGLIFRF
Sbjct:  254 WLMFLQYAACFGVELTVNNVAANYFSDEFDLSTSKAGLVASLFGLMNLFARSLGGIWS-DFLFAKFGGGVTGMRGRFFAQWTALLWEGCFLFLFTFMNQLGAAIPVLILFSVGVQMAEGTSYGIVPYIVPDATGAVSGIVGAGGNFGAVMWGLIFRF 409
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A812SYP8_9DINO (NRT2.5 protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812SYP8_9DINO)

HSP 1 Score: 195 bits (495), Expect = 4.050e-56
Identity = 99/156 (63.46%), Postives = 122/156 (78.21%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRR--ASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            W+LF QYAACFGVE+T+NN+ AGY   ++ LS + AG++ASLFGLMN+FAR++GG  S +  +   +  TGMRGR  AQ  +L+WE + L IFT M+ LG  IGVL+LFSLGVQMAEG ++GIVPYV P ATGAVSG+VGAGGN GAV WGLIFRF
Sbjct:  448 WILFIQYAACFGVEVTMNNILAGYLETKYELSVSLAGLIASLFGLMNLFARAMGGALSDKLFQLFGAGTTGMRGRIYAQSISLVWEGIMLLIFTQMDSLGATIGVLLLFSLGVQMAEGCTFGIVPYVKPTATGAVSGVVGAGGNVGAVCWGLIFRF 603
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A812ST29_9DINO (Nitrate/nitrite transporter n=2 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812ST29_9DINO)

HSP 1 Score: 190 bits (483), Expect = 1.160e-55
Identity = 97/156 (62.18%), Postives = 121/156 (77.56%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRR--ASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            W+LF QYAACFGVE+T+NN+ A Y   ++ LS + AG++ASLFGLMN+FAR++GG  S +  +   +  TGMRGR  AQ  +L+WE + L IFT M+ LG  IGVL+LFSLGVQMAEG ++GIVPYV P ATGAVSG+VGAGGN GAV WGL+FRF
Sbjct:  278 WILFIQYAACFGVEVTMNNILAVYLETKYELSVSLAGLIASLFGLMNLFARAMGGALSDKLFQWFGAGTTGMRGRIYAQSISLVWEGIMLLIFTKMDSLGATIGVLLLFSLGVQMAEGCTFGIVPYVKPTATGAVSGVVGAGGNVGAVCWGLMFRF 433
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A812SSL6_9DINO (Nitrate/nitrite transporter n=3 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812SSL6_9DINO)

HSP 1 Score: 190 bits (483), Expect = 4.000e-55
Identity = 97/156 (62.18%), Postives = 121/156 (77.56%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRR--ASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            W+LF QYAACFGVE+T+NN+ A Y   ++ LS + AG++ASLFGLMN+FAR++GG  S +  +   +  TGMRGR  AQ  +L+WE + L IFT M+ LG  IGVL+LFSLGVQMAEG ++GIVPYV P ATGAVSG+VGAGGN GAV WGL+FRF
Sbjct:  341 WILFIQYAACFGVEVTMNNILAVYLETKYELSVSLAGLIASLFGLMNLFARAMGGALSDKLFQWFGAGTTGMRGRIYAQSISLVWEGIMLLIFTKMDSLGATIGVLLLFSLGVQMAEGCTFGIVPYVKPTATGAVSGVVGAGGNVGAVCWGLMFRF 496
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A812T2A0_9DINO (NRT2.5 protein n=3 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812T2A0_9DINO)

HSP 1 Score: 195 bits (495), Expect = 8.530e-55
Identity = 99/156 (63.46%), Postives = 122/156 (78.21%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRR--ASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            W+LF QYAACFGVE+T+NN+ AGY   ++ LS + AG++ASLFGLMN+FAR++GG  S +  +   +  TGMRGR  AQ  +L+WE + L IFT M+ LG  IGVL+LFSLGVQMAEG ++GIVPYV P ATGAVSG+VGAGGN GAV WGLIFRF
Sbjct:  523 WILFIQYAACFGVEVTMNNILAGYLETKYELSVSLAGLIASLFGLMNLFARAMGGALSDKLFQLFGAGTTGMRGRIYAQSISLVWEGIMLLIFTQMDSLGATIGVLLLFSLGVQMAEGCTFGIVPYVKPTATGAVSGVVGAGGNVGAVCWGLIFRF 678
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A448YWL2_9STRA (Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448YWL2_9STRA)

HSP 1 Score: 172 bits (437), Expect = 3.090e-49
Identity = 93/153 (60.78%), Postives = 110/153 (71.90%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRRASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFR 459
            W+LF QYA CFGVELT+NN AA YF D+FGLST  A  +AS+FG MN+FAR LGG  S +  +   + GMRGR  +Q   LI E V + +F   N LG AI V++ FSL VQ AEGTSYGIVPYV P +TGA+SGIVGAGGN GAV +GL FR
Sbjct:  223 WILFIQYACCFGVELTMNNAAALYFKDKFGLSTESAAAIASIFGFMNLFARGLGGFMSDKGNQ---YLGMRGRIASQTICLIIEGVLVLVFAQTNSLGAAIAVMVFFSLFVQAAEGTSYGIVPYVDPPSTGAISGIVGAGGNTGAVCFGLGFR 372
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A7S2UXC3_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UXC3_9STRA)

HSP 1 Score: 166 bits (419), Expect = 2.380e-48
Identity = 86/158 (54.43%), Postives = 107/158 (67.72%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRRASIFTG----MRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFRF 462
            WLLF QYA CFG+ELT+NN A  Y+ DEFG S   AG+V SLFGLMN+FAR+LGG  S    +   F      ++ R I     L++E + L  F+     G A+GV++LFS+GVQ AEGTS+ +VPYV P  TGAVSGIVGAGGN GA+MWG +F F
Sbjct:   53 WLLFVQYACCFGIELTINNFAVSYYVDEFGQSLEIAGLVGSLFGLMNLFARALGGIISDFANKNLTFLNKNHSLKARMITHCGILLYEGLMLIAFSRATTFGAAVGVMVLFSMGVQCAEGTSFALVPYVDPPVTGAVSGIVGAGGNTGAMMWGFLFLF 210
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A1E7ES00_9STRA (Nitrate/nitrite transporter n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7ES00_9STRA)

HSP 1 Score: 171 bits (433), Expect = 3.610e-48
Identity = 90/153 (58.82%), Postives = 110/153 (71.90%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRRASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFR 459
            W+LF QYA CFGVELT+NN AA YF D+F LST  A  +AS+FG MN+FAR LGG W+ +  + S + GMRGR  A    L+ E + + +F   N LG AI V++ FSL VQ AEGTSYGIVPY+ P ATG++SGIVGAGGN GAV +GL FR
Sbjct:  269 WILFIQYACCFGVELTMNNAAALYFKDKFDLSTESAAAIASIFGFMNLFARGLGG-WASD--KGSAYMGMRGRLWAHTICLVIEGILVLVFAQTNTLGAAIAVMVFFSLFVQAAEGTSYGIVPYIDPPATGSISGIVGAGGNTGAVCFGLGFR 418
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BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Match: A0A448YXD0_9STRA (Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448YXD0_9STRA)

HSP 1 Score: 169 bits (429), Expect = 4.530e-48
Identity = 93/153 (60.78%), Postives = 109/153 (71.24%), Query Frame = 1 
Query:    1 WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFARSLGGGWSGEYRRASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPAIGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGLIFR 459
            W LF QYA CFGVELT+NN AA YF D+F LST  A  +AS+FG MN+FAR LGG +S +  +   + GMRGR  AQ   LI E V + +F   N LG AI V++ FSL VQ AEGTSYGIVPYV P ATG++SGIVGAGGN GAV +GL FR
Sbjct:  223 WFLFIQYACCFGVELTMNNAAALYFKDKFELSTESAAAIASIFGFMNLFARGLGGYFSDKGNQ---YMGMRGRLWAQTICLIGEGVLVLVFAQTNSLGAAITVMVFFSLFVQAAEGTSYGIVPYVDPPATGSISGIVGAGGNTGAVCFGLGFR 372
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig160830.3895.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FUS1_ECTSI1.160e-7678.21Nitrate/nitrite transporter n=2 Tax=Ectocarpus Tax... [more]
D7FUR8_ECTSI1.640e-7678.98Nitrate high affinity transporter n=4 Tax=Ectocarp... [more]
A0A812SYP8_9DINO4.050e-5663.46NRT2.5 protein n=1 Tax=Symbiodinium natans TaxID=8... [more]
A0A812ST29_9DINO1.160e-5562.18Nitrate/nitrite transporter n=2 Tax=Symbiodinium n... [more]
A0A812SSL6_9DINO4.000e-5562.18Nitrate/nitrite transporter n=3 Tax=Symbiodinium n... [more]
A0A812T2A0_9DINO8.530e-5563.46NRT2.5 protein n=3 Tax=Symbiodinium natans TaxID=8... [more]
A0A448YWL2_9STRA3.090e-4960.78Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzsch... [more]
A0A7S2UXC3_9STRA2.380e-4854.43Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A1E7ES00_9STRA3.610e-4858.82Nitrate/nitrite transporter n=1 Tax=Fragilariopsis... [more]
A0A448YXD0_9STRA4.530e-4860.78Nitrate/nitrite transporter n=1 Tax=Pseudo-nitzsch... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig160830contigH-elongata_contig160830:415..1228 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score242.7
Seed ortholog evalue1.2e-61
Seed eggNOG ortholog2880.D7FUS1
Preferred nameNRT2.2
KEGG koko:K02575
KEGG TC2.A.1.8
KEGG Pathwayko00910,map00910
KEGG ModuleM00615
GOsGO:0000325,GO:0001101,GO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0005886,GO:0006810,GO:0006811,GO:0006820,GO:0007275,GO:0008150,GO:0008509,GO:0009507,GO:0009536,GO:0009628,GO:0009705,GO:0009791,GO:0009987,GO:0010035,GO:0010167,GO:0015075,GO:0015103,GO:0015112,GO:0015267,GO:0015291,GO:0015318,GO:0015513,GO:0015698,GO:0015706,GO:0015707,GO:0016020,GO:0022622,GO:0022803,GO:0022804,GO:0022832,GO:0022836,GO:0022857,GO:0031090,GO:0032501,GO:0032502,GO:0032991,GO:0034220,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0048364,GO:0048527,GO:0048528,GO:0048731,GO:0048856,GO:0050896,GO:0051179,GO:0051234,GO:0051716,GO:0055085,GO:0070887,GO:0071229,GO:0071241,GO:0071249,GO:0071705,GO:0071944,GO:0080167,GO:0090696,GO:0098588,GO:0098656,GO:0098805,GO:0099402,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1902170,GO:1990351
EggNOG free text desc.cellular response to nitrate
EggNOG OGs2QPIC@2759,COG2223@1
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Hectar predicted targeting categorysignal peptide
Exons2
Model size462
Cds size462
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929943.6855934-CDS-H-elongata_contig160830:414..6771622929943.6855934-CDS-H-elongata_contig160830:414..677Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig160830 415..677 -
1691679217.6344376-CDS-H-elongata_contig160830:414..6771691679217.6344376-CDS-H-elongata_contig160830:414..677Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig160830 415..677 -
1622929943.6963234-CDS-H-elongata_contig160830:1029..12281622929943.6963234-CDS-H-elongata_contig160830:1029..1228Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig160830 1030..1228 -
1691679217.6475039-CDS-H-elongata_contig160830:1029..12281691679217.6475039-CDS-H-elongata_contig160830:1029..1228Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig160830 1030..1228 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig160830.3895.1prot_H-elongata_contig160830.3895.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig160830 415..1228 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig160830.3895.1

>prot_H-elongata_contig160830.3895.1 ID=prot_H-elongata_contig160830.3895.1|Name=mRNA_H-elongata_contig160830.3895.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=154bp
WLLFAQYAACFGVELTVNNVAAGYFTDEFGLSTTRAGIVASLFGLMNIFA
RSLGGGWSGEYRRASIFTGMRGRFIAQWTALIWEAVFLYIFTLMNDLGPA
IGVLILFSLGVQMAEGTSYGIVPYVVPDATGAVSGIVGAGGNFGAVMWGL
IFRF
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mRNA from alignment at H-elongata_contig160830:415..1228-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig160830.3895.1 ID=mRNA_H-elongata_contig160830.3895.1|Name=mRNA_H-elongata_contig160830.3895.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=814bp|location=Sequence derived from alignment at H-elongata_contig160830:415..1228- (Himanthalia elongata Himel1 dioecious)
TGGCTCCTGTTCGCTCAGTACGCAGCGTGCTTTGGTGTGGAGTTGACCGT
TAACAACGTGGCGGCTGGATACTTCACCGACGAATTCGGCCTGTCTACCA
CAAGAGCTGGAATAGTAGCTTCCCTTTTTGGTCTTATGAACATTTTCGCC
CGTTCGCTCGGTGGCGGGTGGTCTGGTGAGTACCGGCGTGCTAGCATCTG
TCCATTGGCTTACAGTAAGCACAGTGCGTGGATCACTTTTTTATTTCATG
ATGCTTACAAATTTCGAGTAGAAGCCGATAGTGGAAGATATCAAGGGCCA
TGTCCCCTATGTGGGAGAGTAGAGAGACCGCCCGCGAAGAAGAAGTAATC
CCGCCGAGTCAGGCGCGTGCACGCCATAACTTGGTGGCTTTCATTTCTTC
TTCCTCTGTCTAGCACGAGAGCGATATCTGCCTCTGGCTGGACACAGACA
TATTTTTCACGCCATTGTCGCCGCCACTGCTATTTCAGCCCTTCCTTTCG
CCGATTTTGTGAAAACCACAGACTTCCTCTACGCGAAATTCGGCTCTGGA
GTCACCGGCATGCGTGGACGCTTCATCGCGCAGTGGACCGCTCTCATTTG
GGAAGCTGTCTTTCTCTACATATTTACCCTTATGAACGACCTTGGTCCTG
CCATCGGCGTCCTCATCCTCTTCTCTCTCGGGGTACAGATGGCTGAGGGA
ACCTCGTACGGTATCGTTCCCTACGTCGTCCCCGACGCGACGGGGGCTGT
GTCAGGTATCGTGGGTGCTGGCGGTAATTTTGGCGCGGTGATGTGGGGTC
TGATCTTCCGGTTT
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Coding sequence (CDS) from alignment at H-elongata_contig160830:415..1228-

>mRNA_H-elongata_contig160830.3895.1 ID=mRNA_H-elongata_contig160830.3895.1|Name=mRNA_H-elongata_contig160830.3895.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=924bp|location=Sequence derived from alignment at H-elongata_contig160830:415..1228- (Himanthalia elongata Himel1 dioecious)
TGGCTCCTGTTCGCTCAGTACGCAGCGTGCTTTGGTGTGGAGTTGACCGT
TAACAACGTGGCGGCTGGATACTTCACCGACGAATTCGGCCTGTCTACCA
CAAGAGCTGGAATAGTAGCTTCCCTTTTTGGTCTTATGAACATTTTCGCC
CGTTCGCTCGGTGGCGGGTGGTCTGGTGAGTACCGGCGTGCTAGCATCTT
GGCTCCTGTTCGCTCAGTACGCAGCGTGCTTTGGTGTGGAGTTGACCGTT
AACAACGTGGCGGCTGGATACTTCACCGACGAATTCGGCCTGTCTACCAC
AAGAGCTGGAATAGTAGCTTCCCTTTTTGGTCTTATGAACATTTTCGCCC
GTTCGCTCGGTGGCGGGTGGTCTGGTGAGTACCGGCGTGCTAGCATCTTC
ACCGGCATGCGTGGACGCTTCATCGCGCAGTGGACCGCTCTCATTTGGGA
AGCTGTCTTTCTCTACATATTTACCCTTATGAACGACCTTGGTCCTGCCA
TCGGCGTCCTCATCCTCTTCTCTCTCGGGGTACAGATGGCTGAGGGAACC
TCGTACGGTATCGTTCCCTACGTCGTCCCCGACGCGACGGGGGCTGTGTC
AGGTATCGTGGGTGCTGGCGGTAATTTTGGCGCGGTGATGTGGGGTCTGA
TCTTCCGGTTTTCACCGGCATGCGTGGACGCTTCATCGCGCAGTGGACCG
CTCTCATTTGGGAAGCTGTCTTTCTCTACATATTTACCCTTATGAACGAC
CTTGGTCCTGCCATCGGCGTCCTCATCCTCTTCTCTCTCGGGGTACAGAT
GGCTGAGGGAACCTCGTACGGTATCGTTCCCTACGTCGTCCCCGACGCGA
CGGGGGCTGTGTCAGGTATCGTGGGTGCTGGCGGTAATTTTGGCGCGGTG
ATGTGGGGTCTGATCTTCCGGTTT
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