mRNA_H-elongata_contig141815.2892.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig141815.2892.1
Unique NamemRNA_H-elongata_contig141815.2892.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: D7FZF2_ECTSI (Autophagy-related protein 9 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZF2_ECTSI)

HSP 1 Score: 140 bits (354), Expect = 4.090e-37
Identity = 70/79 (88.61%), Postives = 74/79 (93.67%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            + + +SYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHAN FLMRFPRPVLASLMRVTRF SG+VVAVLLVMT LE
Sbjct:  305 WHSSKSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANTFLMRFPRPVLASLMRVTRFVSGSVVAVLLVMTFLE 383
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A6H5K9U8_9PHAE (Autophagy-related protein 9 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9U8_9PHAE)

HSP 1 Score: 139 bits (351), Expect = 1.000e-36
Identity = 69/79 (87.34%), Postives = 74/79 (93.67%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            + + +SYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHAN FLMRFPRPVLAS+MRVTRF SG+VVAVLLVMT LE
Sbjct:  296 WHSSKSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANTFLMRFPRPVLASVMRVTRFVSGSVVAVLLVMTFLE 374
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A836CNL2_9STRA (Autophagy protein Apg9-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNL2_9STRA)

HSP 1 Score: 108 bits (271), Expect = 6.610e-26
Identity = 52/79 (65.82%), Postives = 61/79 (77.22%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            + A + YLGPREWSPLA WTFREFNELQH +++RM+GSY HANA+L  FP P+  S MR  RF SGA VAVLLV T +E
Sbjct:  482 WHASKDYLGPREWSPLANWTFREFNELQHIYDRRMAGSYRHANAYLSAFPAPIWGSAMRTLRFISGAFVAVLLVATAME 560
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A7S3XNV8_HETAK (Autophagy-related protein 9 (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XNV8_HETAK)

HSP 1 Score: 92.8 bits (229), Expect = 2.890e-20
Identity = 45/79 (56.96%), Postives = 59/79 (74.68%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            + AE SYLGPREWS  ARW FREFNEL HFFE+RM+GSY +AN +L +F  P+     R  RF SGA++ VLL++T+++
Sbjct:  439 WHAEGSYLGPREWSNYARWAFREFNELPHFFERRMAGSYKYANLYLAQFSSPLAQLAARGVRFVSGALLTVLLLLTLID 517
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: F0YN89_AURAN (Autophagy-related protein 9 n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YN89_AURAN)

HSP 1 Score: 86.7 bits (213), Expect = 4.470e-18
Identity = 42/70 (60.00%), Postives = 54/70 (77.14%), Query Frame = 1 
Query:   10 EQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLL 219
            ++ YLGPREWSP+A+W+FRE NEL H FE R++ S   ANA++  FPRPV+A+L R   F SGA+VAVLL
Sbjct:  285 KKHYLGPREWSPVAQWSFRELNELPHVFETRLAASRKPANAYVSLFPRPVVATLARCVAFVSGALVAVLL 354
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: I2CRC3_NANGC (Autophagy-related protein 9 (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CRC3_NANGC)

HSP 1 Score: 81.6 bits (200), Expect = 4.920e-17
Identity = 38/79 (48.10%), Postives = 58/79 (73.42%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            ++ +++YLGPR+WSPLA+W FRE+NEL H FE+R+  S+  A  +  + PRPVL  L R   + +G+VVAVLL+ T+++
Sbjct:   53 WQQKKNYLGPRQWSPLAQWRFREYNELPHLFERRLRASHPFAALYTRQTPRPVLGVLARCLAYMTGSVVAVLLLFTLVD 131
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: W7TMH9_9STRA (Autophagy-related protein 9 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TMH9_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 1.020e-16
Identity = 38/79 (48.10%), Postives = 59/79 (74.68%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            ++ +++YLGPR+WSPLA+W FRE+NEL H FE+R+  S+ +A  +  + PRPVL  L R   + +G+VVAVLL+ T+++
Sbjct:  442 WQQKKNYLGPRQWSPLAQWRFREYNELPHLFERRLRASHPYAALYTRQTPRPVLGVLARCLAYMTGSVVAVLLLFTLVD 520
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A4D9D5X3_9STRA (Autophagy-related protein 9 n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D5X3_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 1.030e-16
Identity = 38/79 (48.10%), Postives = 59/79 (74.68%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            ++ +++YLGPR+WSPLA+W FRE+NEL H FE+R+  S+ +A  +  + PRPVL  L R   + +G+VVAVLL+ T+++
Sbjct:  438 WQQKKNYLGPRQWSPLAQWRFREYNELPHLFERRLRASHPYAALYTRQTPRPVLGVLARCLAYMTGSVVAVLLLFTLVD 516
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A6S8BVW5_9STRA (Autophagy-related protein 9 n=2 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A6S8BVW5_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 1.900e-16
Identity = 38/79 (48.10%), Postives = 55/79 (69.62%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            F  +Q+YLGPR WSP + W FREFNEL+HFFE+R   S  HA+A++ +FP P+  ++     F SG+ + VLL+ T+L+
Sbjct:  382 FHLKQNYLGPRGWSPHSLWQFREFNELEHFFERRTRASMKHADAYIKQFPTPIPTTIASGISFISGSFLGVLLLFTLLD 460
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BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Match: A0A7S1U8L1_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U8L1_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 1.930e-16
Identity = 43/79 (54.43%), Postives = 57/79 (72.15%), Query Frame = 1 
Query:    1 FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFPRPVLASLMRVTRFFSGAVVAVLLVMTVLE 237
            +RA  +YLGPREWSPLAR  FREFNEL H FE+R++ +   A  +   F RP+LA+  R+  F SGA+VAVLLV T+++
Sbjct:  390 WRASNNYLGPREWSPLARCMFREFNELPHVFEERLARTIGPATDYQGSFSRPLLATAGRLLSFISGALVAVLLVFTLMD 468
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig141815.2892.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FZF2_ECTSI4.090e-3788.61Autophagy-related protein 9 n=1 Tax=Ectocarpus sil... [more]
A0A6H5K9U8_9PHAE1.000e-3687.34Autophagy-related protein 9 n=1 Tax=Ectocarpus sp.... [more]
A0A836CNL2_9STRA6.610e-2665.82Autophagy protein Apg9-domain-containing protein n... [more]
A0A7S3XNV8_HETAK2.890e-2056.96Autophagy-related protein 9 (Fragment) n=1 Tax=Het... [more]
F0YN89_AURAN4.470e-1860.00Autophagy-related protein 9 n=1 Tax=Aureococcus an... [more]
I2CRC3_NANGC4.920e-1748.10Autophagy-related protein 9 (Fragment) n=1 Tax=Nan... [more]
W7TMH9_9STRA1.020e-1648.10Autophagy-related protein 9 n=1 Tax=Nannochloropsi... [more]
A0A4D9D5X3_9STRA1.030e-1648.10Autophagy-related protein 9 n=1 Tax=Nannochloropsi... [more]
A0A6S8BVW5_9STRA1.900e-1648.10Autophagy-related protein 9 n=2 Tax=Aplanochytrium... [more]
A0A7S1U8L1_9STRA1.930e-1654.43Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig141815contigH-elongata_contig141815:225..1433 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score140.2
Seed ortholog evalue4.2e-31
Seed eggNOG ortholog2880.D7FZF2
Preferred nameATG9A
KEGG koko:K02349,ko:K17907,ko:K19672
KEGG TC9.A.15.1
KEGG Pathwayko04136,ko04137,ko04138,ko04140,map04136,map04137,map04138,map04140
GOsGO:0000003,GO:0000045,GO:0000139,GO:0000272,GO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0000407,GO:0000421,GO:0000422,GO:0001817,GO:0001818,GO:0001894,GO:0001932,GO:0001934,GO:0002165,GO:0002376,GO:0003008,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0005776,GO:0005783,GO:0005789,GO:0005794,GO:0005802,GO:0005975,GO:0005976,GO:0005977,GO:0005980,GO:0006073,GO:0006091,GO:0006112,GO:0006508,GO:0006511,GO:0006807,GO:0006810,GO:0006897,GO:0006907,GO:0006909,GO:0006914,GO:0006950,GO:0006952,GO:0006955,GO:0006979,GO:0006996,GO:0007005,GO:0007033,GO:0007154,GO:0007275,GO:0007552,GO:0007586,GO:0008104,GO:0008150,GO:0008152,GO:0008219,GO:0009056,GO:0009057,GO:0009251,GO:0009267,GO:0009314,GO:0009416,GO:0009453,GO:0009605,GO:0009607,GO:0009617,GO:0009620,GO:0009628,GO:0009653,GO:0009791,GO:0009886,GO:0009893,GO:0009966,GO:0009967,GO:0009968,GO:0009987,GO:0009991,GO:0010008,GO:0010498,GO:0010562,GO:0010604,GO:0010623,GO:0010646,GO:0010647,GO:0010648,GO:0010669,GO:0012501,GO:0012505,GO:0012506,GO:0015980,GO:0016020,GO:0016021,GO:0016043,GO:0016052,GO:0016192,GO:0016236,GO:0016237,GO:0019220,GO:0019222,GO:0019538,GO:0019941,GO:0019954,GO:0022411,GO:0022600,GO:0022607,GO:0023051,GO:0023056,GO:0023057,GO:0030054,GO:0030154,GO:0030163,GO:0030277,GO:0030587,GO:0030659,GO:0031090,GO:0031152,GO:0031153,GO:0031224,GO:0031288,GO:0031323,GO:0031325,GO:0031399,GO:0031401,GO:0031410,GO:0031667,GO:0031668,GO:0031669,GO:0031902,GO:0031982,GO:0031984,GO:0032006,GO:0032007,GO:0032258,GO:0032268,GO:0032270,GO:0032479,GO:0032480,GO:0032501,GO:0032502,GO:0032588,GO:0032648,GO:0032688,GO:0032872,GO:0032874,GO:0033036,GO:0033365,GO:0033554,GO:0034067,GO:0034497,GO:0034599,GO:0034613,GO:0034727,GO:0035069,GO:0035096,GO:0040011,GO:0042175,GO:0042221,GO:0042325,GO:0042327,GO:0042330,GO:0042331,GO:0042592,GO:0042594,GO:0043161,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043408,GO:0043410,GO:0043632,GO:0043933,GO:0044042,GO:0044085,GO:0044237,GO:0044238,GO:0044247,GO:0044248,GO:0044257,GO:0044260,GO:0044262,GO:0044264,GO:0044265,GO:0044267,GO:0044275,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044432,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0044764,GO:0044804,GO:0044805,GO:0045087,GO:0045937,GO:0046328,GO:0046330,GO:0048468,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048707,GO:0048856,GO:0048869,GO:0048871,GO:0050789,GO:0050794,GO:0050832,GO:0050896,GO:0051171,GO:0051173,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051241,GO:0051246,GO:0051247,GO:0051259,GO:0051260,GO:0051603,GO:0051641,GO:0051703,GO:0051704,GO:0051707,GO:0051716,GO:0055037,GO:0055114,GO:0060033,GO:0060249,GO:0060255,GO:0060729,GO:0061709,GO:0061723,GO:0061726,GO:0061912,GO:0061919,GO:0065003,GO:0065007,GO:0065008,GO:0070302,GO:0070304,GO:0070727,GO:0070887,GO:0070925,GO:0071496,GO:0071704,GO:0071840,GO:0072665,GO:0080090,GO:0080134,GO:0080135,GO:0090382,GO:0090702,GO:0097708,GO:0098542,GO:0098588,GO:0098630,GO:0098657,GO:0098743,GO:0098791,GO:0098805,GO:0098827,GO:0098852,GO:0099120,GO:1901564,GO:1901565,GO:1901575,GO:1902531,GO:1902532,GO:1902533,GO:1903008,GO:1905037
EggNOG free text desc.process utilizing autophagic mechanism
EggNOG OGsKOG2173@1,KOG2173@2759
EC2.7.7.7
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02000,ko03029,ko03036,ko03400,ko04131
Hectar predicted targeting categorysignal anchor
Exons2
Model size237
Cds size237
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929842.418334-CDS-H-elongata_contig141815:224..3831622929842.418334-CDS-H-elongata_contig141815:224..383Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141815 225..383 -
1691679179.5795004-CDS-H-elongata_contig141815:224..3831691679179.5795004-CDS-H-elongata_contig141815:224..383Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141815 225..383 -
1622929842.4317315-CDS-H-elongata_contig141815:1355..14331622929842.4317315-CDS-H-elongata_contig141815:1355..1433Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141815 1356..1433 -
1691679179.5970109-CDS-H-elongata_contig141815:1355..14331691679179.5970109-CDS-H-elongata_contig141815:1355..1433Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141815 1356..1433 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig141815.2892.1prot_H-elongata_contig141815.2892.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig141815 225..1433 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig141815.2892.1

>prot_H-elongata_contig141815.2892.1 ID=prot_H-elongata_contig141815.2892.1|Name=mRNA_H-elongata_contig141815.2892.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=79bp
FRAEQSYLGPREWSPLARWTFREFNELQHFFEQRMSGSYVHANAFLMRFP
RPVLASLMRVTRFFSGAVVAVLLVMTVLE
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mRNA from alignment at H-elongata_contig141815:225..1433-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig141815.2892.1 ID=mRNA_H-elongata_contig141815.2892.1|Name=mRNA_H-elongata_contig141815.2892.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1209bp|location=Sequence derived from alignment at H-elongata_contig141815:225..1433- (Himanthalia elongata Himel1 dioecious)
TTTCGCGCTGAACAGAGCTACCTCGGCCCGAGAGAGTGGAGCCCCCTGGC
GAGGTGGACCTTCAGGGAGTTTAACGAGGTGGATGCGTGCGTGAGGAGGA
AGGGCGGGCAGGCGCCAACTGCGCGCTCGCACTGACGCTGACGGTCCACA
CACCGTTGATTCTTGTCCCTCACACACACAGAGACTGTAACGCACTGTAG
ATAGAGGTGACCAACCAGACCAGTTCTGCTTGCTGCTTGCTCTAACGCGT
TCAGCCGCACTCTCGTGGGTGGACCTCTCGTCCAAAGATTTTTCGTATGG
GGGAAGGCGTGTGGGAGGGGAGGGGGGAGACGCGATGCCTTGCACCTGTC
CTCTTATGAGTGCCATTGCTGCTGCTGCTGCTGCTGATGATGATGATGAT
GTTATTTTCTTATTTTGCTGATTGGGGGGGGGGGAAGAAAGAACCCCCCC
CCCTCCTAAAATGAAGAAAAAGGAAAAATATATATTACAAACGAATACAA
AAATAAGTATGGACCTGCAAAATATAAAAAGCTTGATCACACGGCGCTAG
CTTTTGGGAGGGCACGTGTTTATTGGTGAGGGTTATGTCTGGGCTTTTAT
ACGATTTGATCCCAAATGCTTCTCGCCTTCCCCCCCCCCCTCCTCCCCCA
AACACCAACCTTTCTTCATATAAACGCTGTTACTCTTTTGTGNNNNNNNN
NNCCTACCCCAAACACACATTTATTTGGTATAGGAGGCGGTTTCTAATTG
ATGGGATTGCTCTTTTTCAGGATTTGGAGGCTTAGAAAGAGGTGGGATTT
TGCTCTTTTTATCGTTTTTGTCTGTCCTTCCCTCTCTTTCGACTCCATAA
GGGTGTTTATTGATGATTATCTTGCCGCCCACACCCCTTACACACCCCCC
TCTTCCTCCTCCCGAGGACGTGTGGTCAATGGTCTAACAATTAAAGCGTA
CAAACCTAGAGCTTCTTTTTCACAGAAGTTCATTCGTCGTCTTCGTTTAA
CTTCCTTCCCTCCCCCTCTCCCTCTCCAAACCCTTGCCCCTCCGATACAG
CTCCAGCACTTCTTCGAGCAGCGGATGTCTGGCAGCTACGTGCACGCGAA
CGCGTTCCTGATGAGGTTTCCTAGACCCGTGCTGGCGTCCTTGATGCGGG
TGACGAGATTCTTCTCAGGCGCCGTGGTGGCGGTTCTCTTGGTGATGACC
GTACTAGAG
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Coding sequence (CDS) from alignment at H-elongata_contig141815:225..1433-

>mRNA_H-elongata_contig141815.2892.1 ID=mRNA_H-elongata_contig141815.2892.1|Name=mRNA_H-elongata_contig141815.2892.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=474bp|location=Sequence derived from alignment at H-elongata_contig141815:225..1433- (Himanthalia elongata Himel1 dioecious)
TTTCGCGCTGAACAGAGCTACCTCGGCCCGAGAGAGTGGAGCCCCCTGGC
GAGGTGGACCTTCAGGGAGTTTAACGAGTTTCGCGCTGAACAGAGCTACC
TCGGCCCGAGAGAGTGGAGCCCCCTGGCGAGGTGGACCTTCAGGGAGTTT
AACGAGCTCCAGCACTTCTTCGAGCAGCGGATGTCTGGCAGCTACGTGCA
CGCGAACGCGTTCCTGATGAGGTTTCCTAGACCCGTGCTGGCGTCCTTGA
TGCGGGTGACGAGATTCTTCTCAGGCGCCGTGGTGGCGGTTCTCTTGGTG
ATGACCGTACTAGAGCTCCAGCACTTCTTCGAGCAGCGGATGTCTGGCAG
CTACGTGCACGCGAACGCGTTCCTGATGAGGTTTCCTAGACCCGTGCTGG
CGTCCTTGATGCGGGTGACGAGATTCTTCTCAGGCGCCGTGGTGGCGGTT
CTCTTGGTGATGACCGTACTAGAG
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