mRNA_H-elongata_contig13006.2193.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig13006.2193.1
Unique NamemRNA_H-elongata_contig13006.2193.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: D8LB90_ECTSI (Tafazzin family protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LB90_ECTSI)

HSP 1 Score: 192 bits (488), Expect = 2.900e-58
Identity = 86/111 (77.48%), Postives = 102/111 (91.89%), Query Frame = 1 
Query:    1 QVECHNQETLVEAVLNRGDKGLLTVSNHMSVYDDPGLWSALIPFWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            +VECHN++ L++AVL+RGD+GLLTVSNHM VYDDPGLWSALIPFW R GR++MRWA+CTDDIY A+P+L+ +F AGRT+PIKRTRGMEQPLF+AFFQKLE GEWGHI AEG
Sbjct:  181 KVECHNRQALLDAVLDRGDRGLLTVSNHMCVYDDPGLWSALIPFW-RTGRRRMRWALCTDDIYYAHPVLKNIFEAGRTLPIKRTRGMEQPLFKAFFQKLEGGEWGHIFAEG 290
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A6H5KEM7_9PHAE (Tafazzin family protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEM7_9PHAE)

HSP 1 Score: 190 bits (482), Expect = 1.390e-56
Identity = 85/111 (76.58%), Postives = 101/111 (90.99%), Query Frame = 1 
Query:    1 QVECHNQETLVEAVLNRGDKGLLTVSNHMSVYDDPGLWSALIPFWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            +VECHN++ L++AVL+RGD+GLLTVSNHM VYDDPGLWSAL+PFW R GRK+MRWA+CTDDIY A+P+L+ +F AGRT+PIKR RGMEQPLF+AFFQKLE GEWGHI AEG
Sbjct:  190 KVECHNRQELLDAVLDRGDRGLLTVSNHMCVYDDPGLWSALVPFW-RTGRKRMRWALCTDDIYYAHPVLKNIFDAGRTLPIKRNRGMEQPLFKAFFQKLEGGEWGHIFAEG 299
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A7S2UUF3_9STRA (Tafazzin family protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UUF3_9STRA)

HSP 1 Score: 105 bits (263), Expect = 1.100e-24
Identity = 49/112 (43.75%), Postives = 74/112 (66.07%), Query Frame = 1 
Query:    1 QVECHNQETLVEAVLNRGDK-GLLTVSNHMSVYDDPGLWSALIPFWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            +V  H +E L++ V NR    G+L+VSNHMS+YDDPGLWSA+IP+W R   + +R+++C DD+Y  +  L  +F AG+ +P+ R  G  Q   + + + ++EG W HI AEG
Sbjct:  178 KVVTHRKELLMDCVYNREPGVGILSVSNHMSMYDDPGLWSAIIPWW-RMPHRLLRYSLCNDDMYFVSKYLTPIFLAGKALPVSRKAGPNQQFMKDYTEVVDEGNWCHIFAEG 288
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A7J7PEG5_9CHLO (Tafazzin family protein n=1 Tax=Scenedesmus sp. NREL 46B-D3 TaxID=2650976 RepID=A0A7J7PEG5_9CHLO)

HSP 1 Score: 90.1 bits (222), Expect = 5.480e-19
Identity = 47/110 (42.73%), Postives = 64/110 (58.18%), Query Frame = 1 
Query:   16 NQETLVEAVLNRGD-KGLLTVSNHMSVYDDPGLWSALIP---FWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            NQ+T  E V NR    GLLT+SNH S +DDPGL  A++P   FW+     + RW +C  DI   N LL++ F  G+T+P++R  G+ Q +     + L  GEW HI  EG
Sbjct:   46 NQDTYTELVHNRPHGAGLLTISNHTSTFDDPGLPCAMLPWHFFWTEHNHGRNRWTMCASDICFRNELLRQFFLNGKTLPVERGAGVGQRIMGVAARALARGEWLHIFPEG 155
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: C1E8W0_MICCC (Tafazzin family protein n=2 Tax=Micromonas TaxID=38832 RepID=C1E8W0_MICCC)

HSP 1 Score: 88.2 bits (217), Expect = 8.530e-19
Identity = 46/112 (41.07%), Postives = 63/112 (56.25%), Query Frame = 1 
Query:   10 CHNQETLVEAVLNRG-DKGLLTVSNHMSVYDDPGLWSALIP---FWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            C N ET    V  R    GL+TVSNH S +DDPGL S +IP   F S    + +RW +CT +I   N L+ + F AG+T+PI R  G+ Q +     +++  G+W HI  EG
Sbjct:   57 CTNYETFYHHVTQRRRPNGLITVSNHASTFDDPGLLSWMIPWRYFLSEPTHRGIRWTMCTSEICAKNALVHKFFAAGKTVPITRGGGINQAVMGTMAERVSRGDWLHIFPEG 168
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A383WP59_TETOB (Tafazzin family protein n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WP59_TETOB)

HSP 1 Score: 88.6 bits (218), Expect = 1.260e-18
Identity = 46/110 (41.82%), Postives = 64/110 (58.18%), Query Frame = 1 
Query:   16 NQETLVEAVLNRGDK-GLLTVSNHMSVYDDPGLWSALIP---FWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            N ET  E V NR +  GLLT+SNH S +DDPGL  A++P   FW+     K RW +C  DI   N + ++ F  G+T+P++R  G+ Q +     + L +GEW HI  EG
Sbjct:   46 NHETYKELVHNRPEGVGLLTISNHTSTFDDPGLPCAMLPWHFFWTEHSHCKNRWTMCASDICFTNEVFRQFFLNGKTLPVERGAGVGQRIMGVAARALAQGEWLHIFPEG 155
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A1V9ZBT6_9STRA (Elongation factor 3 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZBT6_9STRA)

HSP 1 Score: 89.7 bits (221), Expect = 1.370e-18
Identity = 41/108 (37.96%), Postives = 68/108 (62.96%), Query Frame = 1 
Query:   16 NQETLVEAVLNR-GDKGLLTVSNHMSVYDDPGLWSALIPFWSRAGRKKMRWAICTDDI-YNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            N+++L + +L R   + L+TV+NH S  DDP +++ ++P WS    +  RW +C+ +  Y   PLL  VF A +T+PIKR  G++ P+ E  F+K++ G+W H+  EG
Sbjct: 1130 NEDSLAKWILARPAHRALITVTNHSSTVDDPAVFARILP-WSCVNPRICRWGLCSQEYCYTKGPLLSAVFYASKTLPIKRGAGLDHPMLEDLFKKVQNGQWVHVFPEG 1236
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BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A0D2LY10_9CHLO (Tafazzin family protein n=1 Tax=Monoraphidium neglectum TaxID=145388 RepID=A0A0D2LY10_9CHLO)

HSP 1 Score: 85.1 bits (209), Expect = 2.120e-18
Identity = 39/94 (41.49%), Postives = 57/94 (60.64%), Query Frame = 1 
Query:   61 GLLTVSNHMSVYDDPGLWSALIP---FWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            GLLT SNH S +DDPGL SAL+P   FW+     KMRW +C  D+   N ++++ F  G+ +P++R  G  QP+     ++L  G+W H+  EG
Sbjct:   70 GLLTYSNHTSTFDDPGLPSALLPWWLFWTEHSHGKMRWTMCARDVCFLNDVMRQFFLNGKALPVERGGGAAQPVIGVAARRLAAGDWLHVFPEG 163
[Close]
BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A8J4DAP2_9CHLO (Tafazzin family protein n=6 Tax=Volvox TaxID=3066 RepID=A0A8J4DAP2_9CHLO)

HSP 1 Score: 86.3 bits (212), Expect = 4.690e-18
Identity = 42/116 (36.21%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:    1 QVECHNQETLVEAVLNRG-DKGLLTVSNHMSVYDDPGLWSALIP---FWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTR-GMEQPLFEAFFQKLEEGEWGHIVAEG 333
            + E  NQ+ L+ A+ NR    G+LTV NH S++DDPG+ S ++P   FW    +  +RW++C  ++   N LL++ F +G+T+P++R + G+ QP+       L  G+W H+  EG
Sbjct:   46 KTEIINQDRLINAIENRQHSSGVLTVCNHTSMFDDPGVLSIIMPWSWFWMDPVQHHVRWSLCAKEVCFKNELLRQFFLSGKTLPVERGKAGVHQPVVSLAAMALASGQWVHVFPEG 161
[Close]
BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Match: A0A2W1BMN9_HELAM (Tafazzin family protein n=4 Tax=Heliothinae TaxID=95178 RepID=A0A2W1BMN9_HELAM)

HSP 1 Score: 85.9 bits (211), Expect = 6.190e-18
Identity = 45/107 (42.06%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:   13 HNQETLVEAVLNRGDKGLLTVSNHMSVYDDPGLWSALIPFWSRAGRKKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLEEGEWGHIVAEG 333
            +N+ETL  AV     + LLTVSNH S +DDPGLW  L    S   R +MRW++   DI   N L    F  G+ +P+ R  G+ QP  +   ++L  GEW HI  EG
Sbjct:   47 YNRETLAAAVRRPRGQPLLTVSNHHSCFDDPGLWGVL-DVRSLTSRSRMRWSLAAHDICFTNALHSCFFALGKCVPVVRGAGVHQPAMDFCVERLARGEWVHIFPEG 152
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig13006.2193.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LB90_ECTSI2.900e-5877.48Tafazzin family protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KEM7_9PHAE1.390e-5676.58Tafazzin family protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S2UUF3_9STRA1.100e-2443.75Tafazzin family protein n=1 Tax=Fibrocapsa japonic... [more]
A0A7J7PEG5_9CHLO5.480e-1942.73Tafazzin family protein n=1 Tax=Scenedesmus sp. NR... [more]
C1E8W0_MICCC8.530e-1941.07Tafazzin family protein n=2 Tax=Micromonas TaxID=3... [more]
A0A383WP59_TETOB1.260e-1841.82Tafazzin family protein n=1 Tax=Tetradesmus obliqu... [more]
A0A1V9ZBT6_9STRA1.370e-1837.96Elongation factor 3 n=1 Tax=Thraustotheca clavata ... [more]
A0A0D2LY10_9CHLO2.120e-1841.49Tafazzin family protein n=1 Tax=Monoraphidium negl... [more]
A0A8J4DAP2_9CHLO4.690e-1836.21Tafazzin family protein n=6 Tax=Volvox TaxID=3066 ... [more]
A0A2W1BMN9_HELAM6.190e-1842.06Tafazzin family protein n=4 Tax=Heliothinae TaxID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig13006contigH-elongata_contig13006:6185..8435 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score194.1
Seed ortholog evalue3.4e-47
Seed eggNOG ortholog2880.D8LB90
KEGG rclassRC00004,RC00037
KEGG koko:K13511
KEGG ReactionR09037
KEGG Pathwayko00564,map00564
EggNOG free text desc.cardiolipin acyl-chain remodeling
EggNOG OGsKOG2847@1,KOG2847@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01004
Hectar predicted targeting categoryother localisation
Exons3
Model size333
Cds size333
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig13006.2193.1prot_H-elongata_contig13006.2193.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig13006 6185..8435 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929773.2539222-CDS-H-elongata_contig13006:6184..63211622929773.2539222-CDS-H-elongata_contig13006:6184..6321Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 6185..6321 +
1691679152.2414124-CDS-H-elongata_contig13006:6184..63211691679152.2414124-CDS-H-elongata_contig13006:6184..6321Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 6185..6321 +
1622929773.2657163-CDS-H-elongata_contig13006:7033..71751622929773.2657163-CDS-H-elongata_contig13006:7033..7175Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 7034..7175 +
1691679152.2506928-CDS-H-elongata_contig13006:7033..71751691679152.2506928-CDS-H-elongata_contig13006:7033..7175Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 7034..7175 +
1622929773.277329-CDS-H-elongata_contig13006:8381..84351622929773.277329-CDS-H-elongata_contig13006:8381..8435Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 8382..8435 +
1691679152.26309-CDS-H-elongata_contig13006:8381..84351691679152.26309-CDS-H-elongata_contig13006:8381..8435Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig13006 8382..8435 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig13006.2193.1

>prot_H-elongata_contig13006.2193.1 ID=prot_H-elongata_contig13006.2193.1|Name=mRNA_H-elongata_contig13006.2193.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=111bp
QVECHNQETLVEAVLNRGDKGLLTVSNHMSVYDDPGLWSALIPFWSRAGR
KKMRWAICTDDIYNANPLLQRVFRAGRTMPIKRTRGMEQPLFEAFFQKLE
EGEWGHIVAEG
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mRNA from alignment at H-elongata_contig13006:6185..8435+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig13006.2193.1 ID=mRNA_H-elongata_contig13006.2193.1|Name=mRNA_H-elongata_contig13006.2193.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2251bp|location=Sequence derived from alignment at H-elongata_contig13006:6185..8435+ (Himanthalia elongata Himel1 dioecious)
CAGGTTGAGTGCCACAACCAGGAAACCCTGGTGGAGGCTGTCTTGAACCG
GGGGGACAAGGGACTCTTGACCGTGAGTAACCACATGTCTGTCTACGATG
ACCCCGGCCTGTGGTCGGCGCTCATTCCATTCTGGAGGTGAGGGTGAGGA
TGGAGACCTGGGAAAATCATAACCCGACCTTTCCTGTTTCTTGGGAGGCG
AAACGCGATAGCAGCACAAGGCTGTTCAGATGTTTTGCGGTAACCTACCA
TCCCGTCCGCGTGTAGCATAGCACACGCGCCCATACGTAGGATAACCCAA
TTCAGCTCTAGCCAAAGCCAAAGCCACGACCATCTTTTAAGCATGCACTT
CTGAATCGTGCATTAATTTTGTTTCTTTATGAGTTTTCGAAAAAGAGCAG
TCACTGTCACTTTCACTTTTAATATATAGTATATAGTGCATAGTATATAG
TATATAGTACCGAGAGGTGAGGCAAGGCCGACAAAAATTGTCGATATTTT
TCGTTTCGCGCGTACAGAACATTAAAATAGGGCTATCAGAGGTGTGTTTC
ACCTATTAATAGCAACAGATACAGCCTAGCCTAGCCTAGCCTCCCAACTT
GCGGCTCTACAAATACAAGAGGTTTGTCACACGATGAGGCATGTTCTGGA
GAGGCGCCGCGGTGCCTCTCCAGGCGCACAGCACCGCACATATACCTTAA
TTCAGTACCGCTAACCAGTAACAGAGCCAGGCGATCATCCGTTGTATAAA
CGCGCAAATTTCGTCGCGGTCGCTCCCGGGATACCACCGAACACCGCCCG
ATTACTCAGAGGTACAGCGCTTGTGTGTGTTGTTTGCGTGCCTTGGGAGT
AGAGCTGGCAGAAAAAAAATGCGCTGGGCCATATGCACGGACGACATCTA
CAACGCCAACCCGCTCCTCCAGAGAGTATTTCGCGCCGGGAGGACGATGC
CTATCAAGCGCACCCGCGGGATGGAGCAGCCTCTCTTCGAGGTGCGGGGC
CACGCCGGCGCGAGACGTGCTTACTGTCTTCCGCGTGATGCCTGGCGCGT
GCGTGCGTGGCTAGATACGTCCAACAAGGACGCTTGACACTGAGGACGGG
GTAGGAAGCGTTGAGTGGTCCCCGTTACGGCGTCGAGGTAGTTCCATCAG
CGTATCAAATGATATTTTCCGCATATGAAAGTTCATGAAGTGTGTCCTGT
GAGCTCAGAGACCTTGATCGAAGAGTCTTGTCTCTCCCCAACGGCGTAAA
ACGCAGTCACATTCTATAGGGTGCATATGCCAAACATTGGATTGTTGGAA
ACCCCTAAAGCAATCATAGACTTACGAGAGTAGTCTGCCTGGGTTATTAA
TAAGTATATTACGGTCTGAGTGTAATCCCATTATGTATGTATATCAGTAT
GTGGTTTCGAGTGCGGCGTTTATGCATTCGCCCGGATATCAAAGCCCCCT
TCTGGTACCTGGAGTATAGGACATAGTATAGTACAGTATATTATAGTATA
GTATACTCGAAAGCGATAATGCCCGCTGAATGGACAATACTACGATTTGG
TCACTCGGTGTGTCCAGTGTCCGATTGATCCGTACCATGTCCGGTTTGGC
GTTGCAGAGGCAAGGGCAGAGGCAAGGGTAGTGATGCCTTAAACTTGGTA
CAGTACCATCCTTACGAAGCGCAACTTGGCCGGCGTAAACGCGGTATCAT
GTATCATGTGTAATAGATAATATGAGAAAGTTAGACCCTTTAGATGCGGA
TAGATTACATTCACTGTGAACAATAGAAAGTATATATATATATATATAAG
CTTACGTTTTTACGTTGGATGTCTATGTTGGGATACCGGGATATTTCCCC
CATGGCATAGACGACATGCCTCACCTAATCAGTTAATTCATATTGGGTTC
CTTTAATATCATCTGCTGTGACATAGCCACATATCATCGCGCTGCCATGT
AATACCTATCAACGCTACACGCGTACTACACCGTACTACACCGTACTGCA
CCGTACTACACCGTACTACACCGGACCTCAAACTGTTAATATTTACGATC
ATTATATCGCCCCGTCCGGGCATTGTTGGGATTCGTGGTCTAGACTATGA
CCGTTATCGCCCATTAGCGTACTACATGCCTTTAACATTCGTACACCCCC
CTCTTCCCCAAATCACCGCTCCTGCTCCCCTTTGTCTCTGACCGAAGGCG
TTTTTCCAGAAGTTAGAGGAGGGCGAGTGGGGGCATATCGTGGCCGAGGG
G
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Coding sequence (CDS) from alignment at H-elongata_contig13006:6185..8435+

>mRNA_H-elongata_contig13006.2193.1 ID=mRNA_H-elongata_contig13006.2193.1|Name=mRNA_H-elongata_contig13006.2193.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=666bp|location=Sequence derived from alignment at H-elongata_contig13006:6185..8435+ (Himanthalia elongata Himel1 dioecious)
CAGGTTGAGTGCCACAACCAGGAAACCCTGGTGGAGGCTGTCTTGAACCG
GGGGGACAAGGGACTCTTGACCGTGAGTAACCACATGTCTGTCTACGATG
ACCCCGGCCTGTGGTCGGCGCTCATTCCATTCTGGAGCAGGTTGAGTGCC
ACAACCAGGAAACCCTGGTGGAGGCTGTCTTGAACCGGGGGGACAAGGGA
CTCTTGACCGTGAGTAACCACATGTCTGTCTACGATGACCCCGGCCTGTG
GTCGGCGCTCATTCCATTCTGGAGTAGAGCTGGCAGAAAAAAAATGCGCT
GGGCCATATGCACGGACGACATCTACAACGCCAACCCGCTCCTCCAGAGA
GTATTTCGCGCCGGGAGGACGATGCCTATCAAGCGCACCCGCGGGATGGA
GCAGCCTCTCTTCGAGTAGAGCTGGCAGAAAAAAAATGCGCTGGGCCATA
TGCACGGACGACATCTACAACGCCAACCCGCTCCTCCAGAGAGTATTTCG
CGCCGGGAGGACGATGCCTATCAAGCGCACCCGCGGGATGGAGCAGCCTC
TCTTCGAGGCGTTTTTCCAGAAGTTAGAGGAGGGCGAGTGGGGGCATATC
GTGGCCGAGGGGGCGTTTTTCCAGAAGTTAGAGGAGGGCGAGTGGGGGCA
TATCGTGGCCGAGGGG
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