mRNA_H-elongata_contig12957.2161.1 (mRNA) Himanthalia elongata Himel1 dioecious

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: D7FTL9_ECTSI (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTL9_ECTSI)

HSP 1 Score: 1001 bits (2587), Expect = 0.000e+0
Identity = 481/551 (87.30%), Postives = 511/551 (92.74%), Query Frame = 2

Query:  173 QVSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDREASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTG 1822
            +VSLLAWTTTPWTLPSNLALCVN  FDY+KLRDVKSGKVY CGA+RL QLYP+M TKKYKPAMKA+LMEELGT+KGSELVGLKYE MF+FF DRE SF+VC D YVTN+SGTGIVHQ+PAFGEDDYRVCL HGVI KGE+IPCPVDSNGLFTSEVGEYAGQ VK ADK+LC+ IKA GRLVSKDS  HSYPFCWRSDTPLIYKAVPSWFV VESIRDKLL +NA+ YWVP+FV EKRFQNWLEGAKDWAVSRNRFWGTPMPMW+S+DGEEMVAVGSIAELK+LSGVEVTDLHRE VDHITIPSKQGKG LKR+EEVFDCWFESGSMPYAQLHYPFENKDKFE+NFPADF+AEGLDQTRGWFYTLTVLGAALFDKPA+KNLIVNGLVLASDG+KMSKRLKNYP    +PMKVI++HGAD     LINSPVVRAESLKFKEEGVQ+TVREVLLPWFNAFRFFVQQARRLEMTCG+RFVPNP+AA  S NVMD WIQATLQGLVQFVHTEMKAYRLYTVVPRLV FIEQLTNWYVRLNRNRLKGAEGAESA  G
Sbjct:  229 EVSLLAWTTTPWTLPSNLALCVNKAFDYVKLRDVKSGKVYYCGADRLVQLYPIMATKKYKPAMKAELMEELGTVKGSELVGLKYEPMFKFFADREQSFVVCEDNYVTNESGTGIVHQSPAFGEDDYRVCLAHGVIAKGEEIPCPVDSNGLFTSEVGEYAGQYVKTADKALCDEIKANGRLVSKDSCTHSYPFCWRSDTPLIYKAVPSWFVGVESIRDKLLKANAETYWVPAFVNEKRFQNWLEGAKDWAVSRNRFWGTPMPMWVSEDGEEMVAVGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQLHYPFENKDKFEKNFPADFIAEGLDQTRGWFYTLTVLGAALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYP----DPMKVIDSHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCGDRFVPNPEAAAKSTNVMDNWIQATLQGLVQFVHTEMKAYRLYTVVPRLVEFIEQLTNWYVRLNRNRLKGAEGAESATAG 775          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: A0A7S3XRA7_HETAK (Isoleucine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XRA7_HETAK)

HSP 1 Score: 771 bits (1992), Expect = 2.990e-267
Identity = 368/561 (65.60%), Postives = 436/561 (77.72%), Query Frame = 2

Query:  170 QQVSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDREAS---FIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTC----GERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTGEC 1828
            ++ SLLAWTTTPWTLPSNLALCVN DFDY+K+RDV  GKVY    ERL QLYP MLTKK+KP    +  E L  +KG+ LVG +Y  +F +F  R  S   F V SDGYVT+DSGTGIVHQAPAFGEDDYRVCL HG+I K E+ PCPVD NG FT  V ++ G+++KEAD  +C  +K +GRLV K  Y HSYPFCWRSDTPLIYKAVPSWFV+VE I+++LLA+N Q YWVP+FV+EKRF NWL  AKDWA+SRNRFWGTP+P+W SDD +E+VAVGS+ EL+ LSGV V DLHRE +DHITIPSK+G+G L+R++EVFDCWFESGSMPYAQ HYPFENK++FE  FPADF+AEGLDQTRGWFYTL V+  ALFDKPA+KNLIVNGLVLA DG+KMSKRLKNYP    +P  V+  +GAD     L+NSPVVRAE L+FKEEGV + V++V LPW+NAFRFF+Q ARR E+T     G RF P+  AA  S N  DAWI A  + L++FV  EM AYRLYTVVP L+ F+  LTNWY+RLNR+RLKG +G   A    C
Sbjct:  145 EEASLLAWTTTPWTLPSNLALCVNVDFDYVKVRDVAGGKVYYLAKERLCQLYPQMLTKKWKPEKAGETFEILEELKGAALVGRRYTPLFPYFAARAESHGNFRVVSDGYVTSDSGTGIVHQAPAFGEDDYRVCLEHGLIAKDEEAPCPVDFNGRFTEAVADFQGRHIKEADADICAHLKERGRLVQKAVYDHSYPFCWRSDTPLIYKAVPSWFVSVEKIKERLLANNDQTYWVPAFVKEKRFHNWLRDAKDWAISRNRFWGTPIPLWCSDDYQEIVAVGSVDELEALSGVRVEDLHREHIDHITIPSKEGRGALRRVDEVFDCWFESGSMPYAQQHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAGDGKKMSKRLKNYP----DPSIVVSKYGADALRLYLVNSPVVRAEPLRFKEEGVLAVVKDVFLPWYNAFRFFMQNARRWELTHPEGGGRRFAPDAAAAAGSANPTDAWILAAKEDLIKFVRQEMGAYRLYTVVPELLTFLNHLTNWYIRLNRDRLKGKDGPAEAHAALC 701          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: A0A482S0Q8_9ARCH (Isoleucine--tRNA ligase n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S0Q8_9ARCH)

HSP 1 Score: 757 bits (1955), Expect = 1.930e-261
Identity = 359/553 (64.92%), Postives = 441/553 (79.75%), Query Frame = 2

Query:  173 QVSLLAWTTTPWTLPSNLALCVNPDFDYIKLRD-VKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFID-REASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTG 1822
            +VSLLAWTTTPWTLPSNLALCVNP FDY+K+RD  ++ +V+I   +RL+Q++P +         +A+L E L  MKGSEL+GLKY  +F++F+  +  +F V  D YVT + GTGIVHQAPAFGEDDYRVC+ + ++ KGE+I CPVDSNG FT+EV E++G+ VKEAD  +C  +K KGRLV K+ Y H YPFCWRS+TPLIYKAVPSWFV VE +R +LLA+NAQ YWVP+FVQEKRF NWL  AKDWA+SRNRFWGTP+P+WISDD EEMVA+GS+ EL+ LSG ++TDLH+EF+D ITIPS++GKG L+RI+EVFDCWFESGSMPYAQLHYPFEN D+FE+ FPADF+AEGLDQTRGWFYTL V+   LFDKPA+KNLIVNGLVLA+DG+KMSKRLKNYP    +P+ V+  +GAD     LINSPVVRA+ LKF+E+GV   +R V++PW+N+FRFF Q   R E + G+ FVPN Q A +S+N +D WI A   GLV+FVH EMKAYRLYTVVPRLV FI++L+ WY+RLNR RLKG  G E A  G
Sbjct:  159 EVSLLAWTTTPWTLPSNLALCVNPTFDYVKIRDKTRNNRVFILLEKRLAQIFPEVGKAGCTDEKRAELYEVLQRMKGSELLGLKYTPLFDYFVHIKPNAFFVVGDSYVTEEGGTGIVHQAPAFGEDDYRVCIANNIVGKGEEIVCPVDSNGRFTNEVPEFSGRAVKEADNDICALLKNKGRLVLKEVYAHQYPFCWRSETPLIYKAVPSWFVTVEKLRPQLLANNAQTYWVPNFVQEKRFHNWLADAKDWAISRNRFWGTPIPLWISDDLEEMVAIGSVEELERLSGAKITDLHKEFIDPITIPSQKGKGVLRRIDEVFDCWFESGSMPYAQLHYPFENADRFEKGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYP----DPVLVVNNYGADALRLYLINSPVVRADVLKFQEDGVSEVLRGVMIPWYNSFRFFTQCVDRWEGSTGKTFVPNAQLARSSQNDVDIWILAASLGLVEFVHQEMKAYRLYTVVPRLVEFIDELSKWYLRLNRFRLKGEMGDEDAAVG 707          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: A0A7S2WSX5_9STRA (Isoleucine--tRNA ligase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WSX5_9STRA)

HSP 1 Score: 758 bits (1958), Expect = 4.630e-261
Identity = 354/555 (63.78%), Postives = 442/555 (79.64%), Query Frame = 2

Query:  176 VSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDR--EASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGV---EVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTG 1822
            VSLLAWTTTPWTLPSNLALCVN +FDY+K++DVK+ ++YI    RL QLYPVM  KK+KP   + L   L  +KG +LVG +Y  +F +F+D   E++F V  DGYVT D GTGIVHQAPAFGEDDYRVCL HGVI KG  +PCPVD NG +T+EV ++ GQ+VK+AD ++C+ +KA+GRLV+   Y+HSYPFCWRSDTPLIYKAVPSWFVAVE  +D+LL +N Q YWVP+FV+E RF NWL+ A+DWA+SRNRFWGTP+P+W S+D +E++A+GS+AEL++L+G+    VTDLHRE +D I IPSKQGKG+L+R++EVFDCWFESGSMPYAQLHYPFENK++FE+ FPA F+AEGLDQTRGWFYTL VL  ALFD+PA+ NLIVNG+VLA+DG+KMSKRLKNYP    +P  VI  +GAD     LINSPVVRAE LKF E GV+   R+V+LPW+N FRFF+Q A R ++  G+ F P+   A +S N ++ WI+A LQ L+ FVH EM  YRLYTV+PRLV F+EQL+ WY+RLNR+RL+GA+G E A  G
Sbjct:  228 VSLLAWTTTPWTLPSNLALCVNVEFDYVKIQDVKTERLYILLETRLVQLYPVMGGKKWKPEKNSTLFTVLEKIKGKDLVGKRYTPLFRYFVDSFGESAFRVIEDGYVTADGGTGIVHQAPAFGEDDYRVCLKHGVIAKGVQLPCPVDFNGCYTAEVSDFEGQHVKQADAAICDMLKAQGRLVASAKYLHSYPFCWRSDTPLIYKAVPSWFVAVEQFKDRLLKNNEQTYWVPNFVKEGRFHNWLKEARDWAISRNRFWGTPIPIWASEDMQELIAIGSVAELEELAGLPKGSVTDLHRESIDDIVIPSKQGKGNLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEKGFPAHFIAEGLDQTRGWFYTLMVLSTALFDRPAFSNLIVNGMVLAADGKKMSKRLKNYP----DPNAVIATYGADALRMYLINSPVVRAEPLKFTEPGVKGVARDVILPWYNTFRFFLQNAHRWQLATGKTFRPDAALASSSANPINVWIRAELQSLIAFVHEEMSKYRLYTVMPRLVSFLEQLSKWYLRLNRDRLRGADGEEEALVG 778          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: A0A7S3GN89_9STRA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GN89_9STRA)

HSP 1 Score: 742 bits (1916), Expect = 1.650e-257
Identity = 358/552 (64.86%), Postives = 423/552 (76.63%), Query Frame = 2

Query:  176 VSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSG-KVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDR-EASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTG 1822
            VSLLAWTTTPWTLPSNLALCVN  F+Y+K+ D   G +V+I   +RL Q++P +        ++A L E +  M G +LVGLKY  +F +F+     +F V SD YVT + GTGIVHQAPAFGEDDYRVCL + ++ KGED+PCPVD+NGLFT+EV E+ G+ VKEAD  +C  +K  GRLV K+ Y HSYPFCWRS+TPLIYKAVPSWFV+VE I+ +LLA+N+  YWVP+FVQEKRF NWL  AKDWA+SRNRFWGTP+P+WISDD EE+V +GS+ EL  LSGV VTDLH+E VDHITIPSKQGKG LKR++EVFDCWFESGSMPYAQ+HYPFENK++F   FPADF+AEGLDQTRGWFYTL V+   LFDKPA+KNLIVNGLVLA+DG+KMSKRLKNYP     P  V+   GAD     LINSPVVRA+ LKF++EGV   VR VLLPWFN FRFF Q   R E   G +FVPN + +  S N +D WI A   GLV+FVH EMKAYRLYTVVPRLV FI+QLT WY+RLNR R+KG    E A  G
Sbjct:  230 VSLLAWTTTPWTLPSNLALCVNEKFEYVKIIDKTRGNRVFILLEKRLGQIFPEVNKPNCTDEIRATLYEVVERMTGDKLVGLKYTPLFNYFVATCPQAFRVVSDSYVTEEGGTGIVHQAPAFGEDDYRVCLKNDIVAKGEDLPCPVDANGLFTAEVPEFVGRGVKEADNDICALLKNNGRLVLKEQYAHSYPFCWRSETPLIYKAVPSWFVSVEKIKPQLLANNSLTYWVPAFVQEKRFHNWLADAKDWAISRNRFWGTPIPLWISDDLEEIVTIGSVEELYQLSGVRVTDLHKEHVDHITIPSKQGKGLLKRVDEVFDCWFESGSMPYAQMHYPFENKERFTNGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYPA----PEIVVNECGADALRLYLINSPVVRADVLKFQQEGVVEVVRGVLLPWFNTFRFFTQCVDRWESISGTKFVPNVEVSKGSTNDVDVWILAATVGLVEFVHQEMKAYRLYTVVPRLVDFIDQLTKWYIRLNRFRIKGESSQEEALVG 777          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: W7U130_9STRA (Isoleucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U130_9STRA)

HSP 1 Score: 741 bits (1913), Expect = 6.500e-254
Identity = 361/572 (63.11%), Postives = 435/572 (76.05%), Query Frame = 2

Query:  179 SLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELG-TMKGSELVGLKYEAMFEFFID-------------RE--ASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVE-VTDLHREFVDHITIPSKQGKGH--LKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTGECTF 1834
            SLLAWTTTPWTLPSNLALCVNP+  YIK+RD KSGK Y+    RL QLYP + + K K   K +  E++G    G+E+ G  Y  +F++F++             RE   +F V  D YVT+DSGTGIVHQAP FGEDDYRVCL HGVI KG ++PCPVDSNG FT +V ++ GQ+VK AD  +C+ +KAKGRLV K +Y HSYPFCWRSDTPLIYKAVPSWFVAV ++++KLLA+N + YWVP+FV+E RF NWL  AKDWA+SRNRFWGTP+P+W S+D EE VA+ SI +L+ LSGV+ +TDLHRE VD ITIPS +G  +  L+R++EVFDCWFESGSMPYAQLHYPF+NK++FE NFPADF+AEGLDQTRGWFYTL V+   LFDKP ++NLIVNGLVLASDG+KMSKRLKNYP     P  +++ +GAD     LI+SPVVRAE L+FKEE V   ++ V LPW NAFRFF Q   RLEM  G  F P+P+ A  S NVMD WIQA+LQG+++FVH EM AYRLYTV PRL+ FIE+LTNWYVRLNR RLKG EG E + TG  T 
Sbjct:  217 SLLAWTTTPWTLPSNLALCVNPEIRYIKVRDKKSGKQYVVAEARLVQLYPKLASGKVKEEQKQEWYEQIGEAFLGAEMAGWGYTPLFDYFVEWNEGERAGKEGDGRENLGAFRVLVDAYVTSDSGTGIVHQAPGFGEDDYRVCLAHGVIRKGTNVPCPVDSNGRFTPDVRDFVGQHVKAADGDICQLLKAKGRLVDKATYSHSYPFCWRSDTPLIYKAVPSWFVAVGTVKEKLLANNQKTYWVPAFVKEHRFHNWLADAKDWAISRNRFWGTPIPLWCSEDMEEQVAIESIEQLERLSGVKGITDLHRESVDQITIPSSRGPSYPPLRRVDEVFDCWFESGSMPYAQLHYPFDNKERFEANFPADFIAEGLDQTRGWFYTLMVISTCLFDKPPFQNLIVNGLVLASDGKKMSKRLKNYPP----PDDILKKYGADALRLYLISSPVVRAEPLRFKEEDVLGMIKRVFLPWINAFRFFQQGVARLEMEKGSVFTPSPERARASTNVMDVWIQASLQGMIKFVHDEMAAYRLYTVTPRLLRFIEELTNWYVRLNRTRLKGQEGDEESLTGLSTL 784          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: A0A6V2M295_9STRA (Isoleucine--tRNA ligase n=4 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6V2M295_9STRA)

HSP 1 Score: 739 bits (1908), Expect = 1.340e-253
Identity = 363/558 (65.05%), Postives = 431/558 (77.24%), Query Frame = 2

Query:  170 QQVSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKK-YKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDREAS---FIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGH-LKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESARTGE 1825
            + V+ +AWTTTPWTLPSNLALCV+P  +Y+K+ D KSGK YI    R++Q+YP+M  KK +KP M  +L E   T+ GSELVG KY  +F+FF +   S   F V SD YVT+D+GTGIVHQAPAFGEDDYRVCL H VI KG++IPCPVDSNG+FT  V    G++VK+AD +L   IK  GRLV KD+  HSYPFCWRSDTPLIYKAVPSWFV VE IRDK++ +N + YWVPS V+E RF NWL  A+DWAVSRNRFWGTP+P+W S+D  E+V +GSIAELK+LSGVEV DLHRE VD +TIPSK+  G  L+RI+EVFDCWFESGSMPYAQ HYPFENK++FE+ FPADF+AEGLDQTRGWFYTL VL  ALFDKPA+KNLIVNGLVLA DG+KMSKRLKNYP    +P+ VI  +GAD     LINSPVVRAESLKF+E GV   V+EV LP +NAFRFF+Q   R E   G +FVP+ +    ++N  D WI A  QGL++FVH EMKAYRLYTV+P LV F+ QLTNWYVRLNR+RLKG EG ++    E
Sbjct:  217 ENVNFIAWTTTPWTLPSNLALCVHPTMEYVKVLDKKSGKTYIMAKNRMAQMYPIMNNKKKWKPKMADELFEIQATLPGSELVGKKYTPIFDFFANTPESENYFRVLSDTYVTDDAGTGIVHQAPAFGEDDYRVCLAHNVIKKGQEIPCPVDSNGVFTEAVPPVKGEHVKKADPTLIALIKENGRLVQKDNLDHSYPFCWRSDTPLIYKAVPSWFVKVEEIRDKIVKNNNETYWVPSAVKEGRFHNWLTDARDWAVSRNRFWGTPIPIWASEDMSEIVCIGSIAELKELSGVEVDDLHRETVDSVTIPSKKNPGTVLRRIDEVFDCWFESGSMPYAQKHYPFENKEQFEKGFPADFIAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAGDGKKMSKRLKNYP----DPILVISKYGADALRMYLINSPVVRAESLKFQEGGVLGVVKEVFLPLYNAFRFFLQNVERWEGR-GNKFVPDIEKVRGTKNPTDIWISAATQGLIKFVHEEMKAYRLYTVMPALVTFVTQLTNWYVRLNRDRLKGLEGEDADAEAE 769          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: L1K156_GUITC (Isoleucine--tRNA ligase n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1K156_GUITC)

HSP 1 Score: 736 bits (1899), Expect = 4.200e-252
Identity = 351/548 (64.05%), Postives = 420/548 (76.64%), Query Frame = 2

Query:  179 SLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDR--EASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESA 1813
            S+LAWTTTPWTLPSNLALCV+P  +Y  + DVK+  VY+    RL QLY            K    + L T+KG EL GLKYE +F FF++R  +  F V  D YVT DSGTGIVHQAPAFGEDDYRVCL  GVI KGE +PCPVD++G FT EV ++ GQ+VKEADK++  ++KA GRLV   +  HSYPFCWRSDTPLIYKAVPSWFV VE++++KLLA+N + YWVP FV+EKRF NWL GA+DWA+SRNRFWGTP+P+W S+D EE++ VGS+ EL+ LSGV V DLH++ +DHITIPSK GKG L+R+EEVFDCWFESGSMPYAQ HYPFENK+KFE+ FPADF+AEGLDQTRGWFYTL V+G ALFDKPA+KN+IVNGLVLA DG+KMSKRLKNYP    +P  VI +HGAD     LINSPVVR E L+FKE+GV+  VR+V LPW+NA+RF +Q  RRL+   G     +      S+N MD WIQA   GL+QFV  EM+AYRLYTVVPRL+ F+E LTNWYV++NR RLKG  G E A
Sbjct:  230 SMLAWTTTPWTLPSNLALCVHPTMEYAYVEDVKTSAVYVLAVPRLVQLYK-----------KESEYKVLKTVKGQELRGLKYEPLFNFFVERKGDVGFRVLCDEYVTADSGTGIVHQAPAFGEDDYRVCLKEGVIFKGEHVPCPVDADGRFTEEVSDFQGQHVKEADKAILASLKASGRLVKSGAIKHSYPFCWRSDTPLIYKAVPSWFVKVETLKEKLLANNDKTYWVPDFVKEKRFHNWLAGARDWAISRNRFWGTPIPIWHSEDWEEVICVGSVDELEQLSGVRVNDLHKDSIDHITIPSKMGKGELRRVEEVFDCWFESGSMPYAQCHYPFENKEKFEKTFPADFIAEGLDQTRGWFYTLMVIGTALFDKPAFKNVIVNGLVLAEDGKKMSKRLKNYP----DPELVINSHGADALRMYLINSPVVRGEELRFKEQGVKDVVRDVFLPWYNAYRFLIQVVRRLQSEEGVTVQGSGTGYIQSDNTMDQWIQAAAAGLLQFVQKEMQAYRLYTVVPRLLRFMEDLTNWYVKMNRKRLKGGAGVEEA 762          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: B8C8U0_THAPS (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C8U0_THAPS)

HSP 1 Score: 730 bits (1885), Expect = 1.310e-251
Identity = 353/552 (63.95%), Postives = 425/552 (76.99%), Query Frame = 2

Query:  176 VSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKK-YKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDREAS----FIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKG-HLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAES 1810
            VS +AWTTTPWTLPSN+ALCV+P  +Y+K+ D K+ K YI    RLSQ++P+M  KK +KPAM  +L E   T  G +LVG KY+ +F++F D        F V SD YVT+D+GTG+VHQAPAFGEDDYRVCL H VI+KG D+PCPVDSNG+FT +V E  G +VK+AD +L + IK KGRLV KD+  HSYPFCWRSDTPLIYKAVPSWF+ VE IRD+++ +N + YWVP+ V+E RF +WL  A+DWAVSRNRFWGTP+P+W ++D  E+V +GS+ EL +LSGV V DLH++ VD ITIPSK+  G  L R++EVFDCWFESGSMPYAQ+HYPFENK+KFE  FPADF+AEGLDQTRGWFYTL VL  ALFDKPA+KNLIVNGLVLASDG+KMSKRLKNYP    +P  VI  +GAD     LINSPVVRAESLKF+E GV   V+EV LP +NAFRFFVQ   R E   G +FVP+ +   ++ N  D WI A  QGL++FVH EM AYRLYTV+P LV F+ QLTNWYVRLNR+RLKG EG ES
Sbjct:  218 VSFVAWTTTPWTLPSNIALCVHPAMEYVKILDKKADKRYILAKARLSQIFPIMNNKKKWKPAMAEELYEVEATYVGKDLVGKKYQPVFDYFNDGSPESKEYFRVLSDNYVTDDAGTGVVHQAPAFGEDDYRVCLAHKVIEKGNDLPCPVDSNGMFTEQVPEVKGLHVKKADDNLIKLIKDKGRLVQKDNLDHSYPFCWRSDTPLIYKAVPSWFIKVEEIRDRIVENNKKTYWVPNQVKEGRFHSWLTDARDWAVSRNRFWGTPIPIWCNEDMTEVVCIGSVDELAELSGVRVDDLHKDVVDKITIPSKKNPGTELHRVDEVFDCWFESGSMPYAQMHYPFENKEKFENGFPADFIAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYP----DPNVVISKYGADALRMYLINSPVVRAESLKFQESGVLGVVKEVFLPLYNAFRFFVQNTERWEAKNGSKFVPSKEKVQSTANPTDVWISAATQGLIKFVHEEMAAYRLYTVMPALVSFVTQLTNWYVRLNRDRLKGLEGGES 765          

BLAST of mRNA_H-elongata_contig12957.2161.1 vs. uniprot
Match: W2Q337_PHYPN (Isoleucine--tRNA ligase n=29 Tax=Peronosporaceae TaxID=4777 RepID=W2Q337_PHYPN)

HSP 1 Score: 733 bits (1893), Expect = 2.980e-251
Identity = 360/553 (65.10%), Postives = 423/553 (76.49%), Query Frame = 2

Query:  158 LAHLQQVSLLAWTTTPWTLPSNLALCVNPDFDYIKLRDVKSGKVYICGAERLSQLYPVMLTKKYKPAMKADLMEELGTMKGSELVGLKYEAMFEFFIDREASFIVCSDGYVTNDSGTGIVHQAPAFGEDDYRVCLLHGVIDKGEDIPCPVDSNGLFTSEVGEYAGQNVKEADKSLCEAIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPSFVQEKRFQNWLEGAKDWAVSRNRFWGTPMPMWISDDGEEMVAVGSIAELKDLSGVEVTDLHREFVDHITIPSKQGKGHLKRIEEVFDCWFESGSMPYAQLHYPFENKDKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGRKMSKRLKNYPGKFYNPMKVIEAHGADXXXX-LINSPVVRAESLKFKEEGVQSTVREVLLPWFNAFRFFVQQARRLEMTCGERFVPNPQAACNSENVMDAWIQATLQGLVQFVHTEMKAYRLYTVVPRLVGFIEQLTNWYVRLNRNRLKGAEGAESA 1813
            LA    V+LLAWTTTPWTLPSNLALCVN  FDY+K++D+K+ KVYI    RL Q+YP M  K YK        E L   KGS LVG KY  +F+ F D   +F V SD YV++  GTGIVHQAP FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G +VK+AD  +C+ +K +GRLVSK + VHSYPFC+RS TPLIY+A+P WFV VE IRD+++A+N   YWVPSFVQEKRF NWL   KDW +SR RFWGTP+P+W+SDD EE+V VGSIAEL++L+G +VTDLHREF+DH+TIPSKQGKG L+R+ EVFDCWFESGSMPYAQ HYPFENKDKFE NFPADFVAEGLDQTRGWFYTL VL  ALFDKPA+KNLIVNGLVLA DGRKMSK LKN    F +P ++++ +GAD     LINSPVVRAE LKF+  GV   +RE+ LPWFN+ RFF QQA RL++  G  FVPN +AA  S NVMD+WI A +  L++FVH EMKAYRLYTVVPRLV FI QLTNWYVRLNR RLKG+ G+  A
Sbjct:  218 LADEPDVALLAWTTTPWTLPSNLALCVNESFDYVKIKDIKTEKVYILDEARLCQVYPKMSKKGYKGGE----FEILEKFKGSTLVGKKYVPLFDCFKDWPNAFRVLSDNYVSDSGGTGIVHQAPTFGEDDYRVCVREGVADKFT-LPDPLDDNGVFTDAVPLVKGLHVKKADDVICQDLKNRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIVANNKLTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELEELAGEKVTDLHREFIDHLTIPSKQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKDKFEANFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKN----FTDPEEILQKYGADALRLYLINSPVVRAEPLKFQAPGVLGVIREIFLPWFNSARFFAQQATRLQLETGVAFVPNREAALASTNVMDSWIIAAMHNLIKFVHEEMKAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEA 761