mRNA_H-elongata_contig101571.135.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig101571.135.1
Unique NamemRNA_H-elongata_contig101571.135.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: D7FPB2_ECTSI (Proteasome subunit alpha type n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPB2_ECTSI)

HSP 1 Score: 189 bits (480), Expect = 1.180e-58
Identity = 93/96 (96.88%), Postives = 95/96 (98.96%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFK 321
            DRLIDPTSVTNIFK+T+KIGCLMTGMIADSRVLVQRLRYEAHEFRFK GYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFK
Sbjct:   56 DRLIDPTSVTNIFKITDKIGCLMTGMIADSRVLVQRLRYEAHEFRFKYGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFK 151
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A835YUN1_9STRA (Proteasome subunit alpha type n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YUN1_9STRA)

HSP 1 Score: 161 bits (407), Expect = 1.300e-47
Identity = 76/97 (78.35%), Postives = 86/97 (88.66%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            DRLIDP SVTN+F+VT+++GCLMTGM AD+R  VQRLRYEAHEFRFK GYD P   LARR ADI QVYTQQAS+RALACVA+L+AVDDEKGPQ+FKV
Sbjct:   56 DRLIDPASVTNLFRVTDRVGCLMTGMTADARASVQRLRYEAHEFRFKYGYDVPVGTLARRFADICQVYTQQASLRALACVALLVAVDDEKGPQVFKV 152
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A7S2WQZ1_9STRA (Proteasome subunit alpha type n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WQZ1_9STRA)

HSP 1 Score: 147 bits (370), Expect = 5.350e-42
Identity = 69/97 (71.13%), Postives = 83/97 (85.57%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            DRLIDP+SVT ++++TEKIGCLMTG+ AD RV V RLR EAHEF+F  GYD P HVLA+RIADI+QVYTQ+AS+R LAC  +L +VDDEKGPQLFK+
Sbjct:   57 DRLIDPSSVTYVYQITEKIGCLMTGLTADCRVQVTRLRQEAHEFKFNYGYDMPVHVLAKRIADISQVYTQEASLRMLACNVILSSVDDEKGPQLFKI 153
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: H3G9M2_PHYRM (Proteasome subunit alpha type n=26 Tax=Oomycota TaxID=4762 RepID=H3G9M2_PHYRM)

HSP 1 Score: 144 bits (363), Expect = 5.620e-41
Identity = 66/98 (67.35%), Postives = 82/98 (83.67%), Query Frame = 1 
Query:   31 QDRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            QD+LI+P SVTN+F++T +IGCLMTG+ ADS+  VQRLR+EAHEF  K GY  P HVLA+RIAD+AQVYTQ ASMRA   + ML++VD+EKGPQLFK+
Sbjct:   54 QDKLIEPDSVTNVFQITPEIGCLMTGLYADSKAQVQRLRFEAHEFENKFGYKIPCHVLAKRIADVAQVYTQHASMRAFGVITMLVSVDEEKGPQLFKI 151
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A067BYZ3_SAPPC (Proteasome subunit alpha type n=4 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067BYZ3_SAPPC)

HSP 1 Score: 144 bits (363), Expect = 5.620e-41
Identity = 68/97 (70.10%), Postives = 81/97 (83.51%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            D+LIDP SVTNIFK+T  IGCLMTG+ AD++  VQRLRYEAH+F  K GY  P HVLA+R+ADI+QVYTQ ASMRAL  V MLI+VD+EKGPQL+K+
Sbjct:   55 DKLIDPDSVTNIFKITPLIGCLMTGLYADAKAQVQRLRYEAHDFEHKYGYPVPCHVLAKRLADISQVYTQHASMRALGVVTMLISVDEEKGPQLYKI 151
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A7S2XVL7_9STRA (Proteasome subunit alpha type n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XVL7_9STRA)

HSP 1 Score: 144 bits (362), Expect = 8.620e-41
Identity = 66/97 (68.04%), Postives = 82/97 (84.54%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            DRLIDP SVT++++V  KIGCLMTGM AD+RV+VQRLRYEA E+RFK+GYD P HVLARR+AD++QVYTQ A +RA+A   +L  VDDEKGP LF++
Sbjct:   57 DRLIDPESVTSLYQVNNKIGCLMTGMTADARVVVQRLRYEAAEYRFKHGYDVPVHVLARRLADLSQVYTQHAGIRAMAATTILAGVDDEKGPMLFRI 153
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: F0W2W5_9STRA (Proteasome subunit alpha type n=2 Tax=Albugo TaxID=65356 RepID=F0W2W5_9STRA)

HSP 1 Score: 143 bits (360), Expect = 1.590e-40
Identity = 68/98 (69.39%), Postives = 81/98 (82.65%), Query Frame = 1 
Query:   31 QDRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            QD+LIDP SVT+IF++T +IGCLMTGM AD++  VQRLRYEA+EF FK GY  P +VLA+R ADIAQVYTQ ASMRA   V MLI +DDEKGPQL+K+
Sbjct:   54 QDKLIDPESVTHIFQITAEIGCLMTGMYADAKAQVQRLRYEANEFAFKFGYSVPCYVLAKRAADIAQVYTQHASMRAFGVVTMLIGIDDEKGPQLYKI 151
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A6G0XHM2_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XHM2_9STRA)

HSP 1 Score: 143 bits (360), Expect = 1.970e-40
Identity = 68/97 (70.10%), Postives = 80/97 (82.47%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            D+LIDP SVTNIFK+T  IGCLMTG+ AD++  VQRLRYEAHEF  K GY  P HVLA+R+ADI+QVYTQ ASMRAL  V +LI +D+EKGPQLFK+
Sbjct:   63 DKLIDPDSVTNIFKITPAIGCLMTGLYADAKAQVQRLRYEAHEFENKFGYAVPVHVLAKRLADISQVYTQHASMRALGVVTILIGIDEEKGPQLFKI 159
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A4D9D8R2_9STRA (PROTEASOME_ALPHA_1 domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D8R2_9STRA)

HSP 1 Score: 150 bits (380), Expect = 3.620e-40
Identity = 72/97 (74.23%), Postives = 84/97 (86.60%), Query Frame = 1 
Query:   34 DRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            DRLIDP+SV+NI+++T+K+G +MTGM  DSR  V RLR EAHEFRFK GYD P HVLA+RIADIAQVYTQQASMRALA V ML+ VDDE+GPQL+KV
Sbjct:   82 DRLIDPSSVSNIYQITDKLGAVMTGMTTDSRAQVTRLRQEAHEFRFKYGYDIPVHVLAKRIADIAQVYTQQASMRALATVCMLVGVDDERGPQLYKV 178
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BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Match: A0A2D4BD13_PYTIN (Proteasome subunit alpha type n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BD13_PYTIN)

HSP 1 Score: 139 bits (349), Expect = 7.250e-39
Identity = 66/98 (67.35%), Postives = 78/98 (79.59%), Query Frame = 1 
Query:   31 QDRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRYEAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDEKGPQLFKV 324
            QD+LIDP SVT++F +T  IG L+TG+ AD+R  VQRLRYEAH+F  K GY  P HVLA+RIAD+AQVYTQ ASMRA   +  LI+VDDEKGPQLFKV
Sbjct:   54 QDKLIDPDSVTSVFPITPSIGALVTGLPADARAQVQRLRYEAHDFEHKYGYKIPCHVLAKRIADVAQVYTQHASMRAFGVITFLISVDDEKGPQLFKV 151
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig101571.135.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FPB2_ECTSI1.180e-5896.88Proteasome subunit alpha type n=1 Tax=Ectocarpus s... [more]
A0A835YUN1_9STRA1.300e-4778.35Proteasome subunit alpha type n=1 Tax=Tribonema mi... [more]
A0A7S2WQZ1_9STRA5.350e-4271.13Proteasome subunit alpha type n=2 Tax=Rhizochromul... [more]
H3G9M2_PHYRM5.620e-4167.35Proteasome subunit alpha type n=26 Tax=Oomycota Ta... [more]
A0A067BYZ3_SAPPC5.620e-4170.10Proteasome subunit alpha type n=4 Tax=Saprolegniac... [more]
A0A7S2XVL7_9STRA8.620e-4168.04Proteasome subunit alpha type n=1 Tax=Fibrocapsa j... [more]
F0W2W5_9STRA1.590e-4069.39Proteasome subunit alpha type n=2 Tax=Albugo TaxID... [more]
A0A6G0XHM2_9STRA1.970e-4070.10Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A4D9D8R2_9STRA3.620e-4074.23PROTEASOME_ALPHA_1 domain-containing protein n=1 T... [more]
A0A2D4BD13_PYTIN7.250e-3967.35Proteasome subunit alpha type n=1 Tax=Pythium insi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig101571contigH-elongata_contig101571:1247..2264 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score192.2
Seed ortholog evalue1.3e-46
Seed eggNOG ortholog2880.D7FPB2
Preferred namePSMA6
KEGG koko:K02730
KEGG Pathwayko03050,map03050
KEGG ModuleM00337,M00340
GOsGO:0000502,GO:0000932,GO:0003674,GO:0003676,GO:0003723,GO:0003735,GO:0003824,GO:0004175,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005739,GO:0005773,GO:0005774,GO:0005829,GO:0005839,GO:0005840,GO:0005844,GO:0006355,GO:0006464,GO:0006508,GO:0006511,GO:0006807,GO:0007088,GO:0007275,GO:0007346,GO:0007517,GO:0007519,GO:0008134,GO:0008150,GO:0008152,GO:0008233,GO:0009056,GO:0009057,GO:0009888,GO:0009889,GO:0009896,GO:0009987,GO:0010035,GO:0010038,GO:0010043,GO:0010468,GO:0010498,GO:0010499,GO:0010556,GO:0010564,GO:0010638,GO:0010965,GO:0012505,GO:0014706,GO:0016020,GO:0016363,GO:0016579,GO:0016787,GO:0019219,GO:0019222,GO:0019538,GO:0019773,GO:0019941,GO:0022626,GO:0030016,GO:0030017,GO:0030071,GO:0030163,GO:0031090,GO:0031323,GO:0031326,GO:0031331,GO:0031347,GO:0031597,GO:0031974,GO:0031981,GO:0032101,GO:0032270,GO:0032436,GO:0032501,GO:0032502,GO:0032991,GO:0033043,GO:0033044,GO:0033045,GO:0033047,GO:0034399,GO:0034515,GO:0035639,GO:0035770,GO:0036211,GO:0036464,GO:0042175,GO:0042221,GO:0042788,GO:0043161,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043292,GO:0043412,GO:0043632,GO:0043687,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044437,GO:0044444,GO:0044445,GO:0044446,GO:0044449,GO:0044464,GO:0045732,GO:0045787,GO:0045840,GO:0045842,GO:0045862,GO:0045931,GO:0048513,GO:0048518,GO:0048522,GO:0048583,GO:0048731,GO:0048856,GO:0050727,GO:0050789,GO:0050794,GO:0050896,GO:0051059,GO:0051090,GO:0051091,GO:0051092,GO:0051128,GO:0051130,GO:0051171,GO:0051247,GO:0051252,GO:0051603,GO:0051726,GO:0051781,GO:0051783,GO:0051785,GO:0051983,GO:0051984,GO:0060255,GO:0060537,GO:0060538,GO:0061061,GO:0062033,GO:0065007,GO:0065009,GO:0070011,GO:0070013,GO:0070646,GO:0070647,GO:0071704,GO:0080090,GO:0080134,GO:0090068,GO:0097159,GO:0098588,GO:0098805,GO:0099080,GO:0099081,GO:0099512,GO:0140096,GO:1901265,GO:1901363,GO:1901564,GO:1901565,GO:1901575,GO:1901800,GO:1901970,GO:1901987,GO:1901989,GO:1901990,GO:1901992,GO:1902099,GO:1902101,GO:1902494,GO:1903052,GO:1903364,GO:1903506,GO:1905368,GO:1905369,GO:1905818,GO:1905820,GO:1990904,GO:2000112,GO:2001141,GO:2001252
EggNOG free text desc.threonine-type endopeptidase activity
EggNOG OGsCOG0638@1,KOG0182@2759
EC3.4.25.1
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01002,ko03051
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionNucleophile aminohydrolases, N-terminal
Ec32 orthologEc-15_004060.2
Exons2
Model size324
Cds size324
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929554.8349931-CDS-H-elongata_contig101571:1246..13611622929554.8349931-CDS-H-elongata_contig101571:1246..1361Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig101571 1247..1361 +
1691679067.944633-CDS-H-elongata_contig101571:1246..13611691679067.944633-CDS-H-elongata_contig101571:1246..1361Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig101571 1247..1361 +
1622929554.8520162-CDS-H-elongata_contig101571:2055..22641622929554.8520162-CDS-H-elongata_contig101571:2055..2264Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig101571 2056..2264 +
1691679067.9564595-CDS-H-elongata_contig101571:2055..22641691679067.9564595-CDS-H-elongata_contig101571:2055..2264Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig101571 2056..2264 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig101571.135.1prot_H-elongata_contig101571.135.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig101571 1247..2264 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig101571.135.1

>prot_H-elongata_contig101571.135.1 ID=prot_H-elongata_contig101571.135.1|Name=mRNA_H-elongata_contig101571.135.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=108bp
MIFVFIFAFCQDRLIDPTSVTNIFKVTEKIGCLMTGMIADSRVLVQRLRY
EAHEFRFKNGYDCPAHVLARRIADIAQVYTQQASMRALACVAMLIAVDDE
KGPQLFKV
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mRNA from alignment at H-elongata_contig101571:1247..2264+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig101571.135.1 ID=mRNA_H-elongata_contig101571.135.1|Name=mRNA_H-elongata_contig101571.135.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1018bp|location=Sequence derived from alignment at H-elongata_contig101571:1247..2264+ (Himanthalia elongata Himel1 dioecious)
ATGATTTTTGTTTTTATTTTTGCTTTTTGTCAGGATCGGCTGATCGACCC
GACCTCAGTGACGAACATTTTCAAGGTTACCGAGAAGATCGGTTGCCTCA
TGACGGGCATGATAGGTGAGAAGAAAAGAAAGGGGATTGATCGATCGATC
GATGGTGATGAAAGCAAGGTTGCGCTCAATGGGATATTTTGTCTGGCCGT
GTTTCGACGCTCAATGCTCGTGGGCGACGGGGAGAGAGTAGGGGATATAT
GTCGGAAGACGGGTTTACGCTCCGTGGAAGGTGCCGTCCTACGCCGTGTA
CGAAGGCAAGCCCACGATTCAAGCTCTTGTCGCGATGTGGGTGGATGGGG
ATTGGAAAAAAAGAATGTGGAAGGGAATGTATTCGAAGAAGAGAGAGCAG
AGCACCCACCCTCCCCCCTGGCCTTTTTATTCATGGTGATCCTACTGATA
TCATGGCGTGTGTCCCGCCTTCCATCTCGTACAACTCCCAATACCTTCTG
CTCTCCATGCTCGGGCGGTTTTTTGGGGGGGTTTTCTCTCCCGTCGCAGT
GGATATGATACTAAAAGAGCTTCTGGTAATTTTTATACTACCGGATGCCA
AGGTCACAAACGAACTGCGTTACCTTCGGCGCCATCTTTCGGTCGACGAT
TATTTTCACCAATTTCATGGGTAATGACCAGTCCCTCGCCCTTCTCCCCG
CCCGCCCGCCCGCCCCCCTGTGATCCCTCCTTATCTTTATGTGCCCTCTT
TGGCGCCGTTGGGGTGGTCTCTCCCGTGTCTACTGGCGTCCTCTGACGGA
AAATTCCAGCCGACTCAAGGGTGCTGGTGCAGCGGCTGCGGTACGAGGCC
CACGAGTTTCGCTTCAAGAACGGGTACGACTGTCCCGCGCATGTGCTGGC
CCGGCGCATCGCCGACATCGCGCAGGTTTACACGCAGCAGGCGTCAATGC
GCGCGCTGGCGTGCGTCGCCATGCTGATCGCGGTGGACGACGAGAAGGGC
CCACAGCTCTTCAAGGTA
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Coding sequence (CDS) from alignment at H-elongata_contig101571:1247..2264+

>mRNA_H-elongata_contig101571.135.1 ID=mRNA_H-elongata_contig101571.135.1|Name=mRNA_H-elongata_contig101571.135.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=648bp|location=Sequence derived from alignment at H-elongata_contig101571:1247..2264+ (Himanthalia elongata Himel1 dioecious)
ATGATTTTTGTTTTTATTTTTGCTTTTTGTCAGGATCGGCTGATCGACCC
GACCTCAGTGACGAACATTTTCAAGGTTACCGAGAAGATCGGTTGCCTCA
TGACGGGCATGATAGATGATTTTTGTTTTTATTTTTGCTTTTTGTCAGGA
TCGGCTGATCGACCCGACCTCAGTGACGAACATTTTCAAGGTTACCGAGA
AGATCGGTTGCCTCATGACGGGCATGATAGCCGACTCAAGGGTGCTGGTG
CAGCGGCTGCGGTACGAGGCCCACGAGTTTCGCTTCAAGAACGGGTACGA
CTGTCCCGCGCATGTGCTGGCCCGGCGCATCGCCGACATCGCGCAGGTTT
ACACGCAGCAGGCGTCAATGCGCGCGCTGGCGTGCGTCGCCATGCTGATC
GCGGTGGACGACGAGAAGGGCCCACAGCTCTTCAAGGTACCGACTCAAGG
GTGCTGGTGCAGCGGCTGCGGTACGAGGCCCACGAGTTTCGCTTCAAGAA
CGGGTACGACTGTCCCGCGCATGTGCTGGCCCGGCGCATCGCCGACATCG
CGCAGGTTTACACGCAGCAGGCGTCAATGCGCGCGCTGGCGTGCGTCGCC
ATGCTGATCGCGGTGGACGACGAGAAGGGCCCACAGCTCTTCAAGGTA
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