mRNA_H-akashiwo_Contig981.2.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig981.2.1
Unique NamemRNA_H-akashiwo_Contig981.2.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A482SNF4_9ARCH (Fatty acid hydroxylase family protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SNF4_9ARCH)

HSP 1 Score: 174 bits (440), Expect = 1.180e-50
Identity = 81/182 (44.51%), Postives = 115/182 (63.19%), Query Frame = 1
Query:   79 PKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            P  A  H +   +NL +A+ F     E  VR    +T+++F +    L  +F  ++ A  ++S+ EYYWH LMH    Y  FHK HH YK P PFDDMYIHP+E+FGYYC  +AP  +  +  VSF  Y++I+G  GV+DHSG    +P +YN+ DH++HH  F VNY+FPFP++D+LH T+
Sbjct:   63 PHRAPLHSLITCVNLFIASCFALMATECSVRRVNRMTYEDFDVNL--LPTIFCQVLLACTYESLAEYYWHRLMHLKFFYIAFHKMHHQYKAPEPFDDMYIHPVEAFGYYCILYAPPFLFTMHYVSFIAYMVIMGIFGVMDHSGIKFSIPAIYNTVDHDSHHAKFEVNYSFPFPYLDILHGTF 242          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: W7TYR2_9STRA (C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TYR2_9STRA)

HSP 1 Score: 167 bits (422), Expect = 1.470e-47
Identity = 86/190 (45.26%), Postives = 121/190 (63.68%), Query Frame = 1
Query:   73 RAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRG--DTFLTFDEFGITAVDLRKVFLG----LVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            RAP+    H +F   NL +A+LF   T E  VRG      T     +   ++    LG    +V +L +QS  EYYWH LMH GP+Y+RFHK HH YK P PFDDMYI P+E+ GYY   ++PA VL +  +SF++Y+ +LG CG++DHSG  +++P +Y++  H+ HH + N N+ FPFPF+D+LH TY
Sbjct:  111 RAPQ----HALFATCNLMIASLFALLTAEGTVRGIGSQLKTGHPHVLGTREVIWWLLGESWLVVASLGWQSAWEYYWHRLMHYGPVYRRFHKHHHFYKAPAPFDDMYIAPLEALGYYFILYSPALVLPMHVLSFAVYLTLLGLCGILDHSGIDVRLPRVYDTRFHDIHHSYTNANFGFPFPFLDVLHGTY 296          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A6G0WDA8_9STRA (Fatty acid hydroxylase domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WDA8_9STRA)

HSP 1 Score: 165 bits (417), Expect = 2.160e-47
Identity = 87/208 (41.83%), Postives = 123/208 (59.13%), Query Frame = 1
Query:    1 LFFGYILFSIGLALQHIKKGDQVVRAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            +FFGYI +S  L +   +       APK +  H   V  NL +AA     + E  +RG + +TFD     A  +R++    + A VF++  EYYWH L+H   +Y R HK HHHYK P+PFDDMY+HP E   YY   + P  V  +   +F++Y+II+GT GV+DHSG   ++P++YNS+DH  HH    VN  FPFP+ D+LH T+
Sbjct:   36 VFFGYISYSSLLHVIFYRNAP----APKRSVHHVTLVSFNLLLAATCAMVSTELHMRGHSQMTFDASPSIATIVRQI----IVACVFENTVEYYWHCLLHCRFLYTRVHKVHHHYKRPQPFDDMYMHPFEGAVYYLVLYGPPFVFSMHVSAFAIYMIIMGTFGVLDHSGLPFRIPLVYNSEDHAMHHAKVIVNLGFPFPYWDILHGTF 235          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A1V9YQH7_9STRA (Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YQH7_9STRA)

HSP 1 Score: 159 bits (401), Expect = 4.530e-45
Identity = 86/208 (41.35%), Postives = 121/208 (58.17%), Query Frame = 1
Query:    1 LFFGYILFSIGLALQHIKKGDQVVRAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            +F GYI  SI   L+ +       RA   A  H      NL VA++F   T E   RG + ++F     TA+ L +V    + A VF++  EYYWH ++HT  MY R HK HHHYK PRPFDDMY+HP E   YY   + P  VL +   SF +Y++++GT G++DHSG  +++P +Y++ DH+ HH     NY FPF  +D++H TY
Sbjct:   36 VFLGYIGSSI--LLERLFYPAPSPRAQPKAADHNALALFNLLVASVFAMATAECHARGLSRMSFGPAPSTAMLLLQV----LGACVFENTIEYYWHRILHTRAMYTRIHKVHHHYKRPRPFDDMYMHPCEGMAYYWVLYGPPFVLPMHLHSFVVYMVLMGTFGLLDHSGVPVRIPFVYDTRDHDVHHTRVTYNYGFPFQLLDVVHGTY 237          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A485KEW3_9STRA (Aste57867_4084 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KEW3_9STRA)

HSP 1 Score: 159 bits (402), Expect = 5.740e-45
Identity = 82/205 (40.00%), Postives = 120/205 (58.54%), Query Frame = 1
Query:   10 GYILFSIGLALQHIKKGDQVVRAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            GY ++S  L +   +     VR+P     H   V  NL +AA F   + E  VRG + + FD+   ++     V    V A++  ++ EYYWH L+H   +Y R HK HHHYK P+PFDDMY+HP E   Y+C  + P  VL ++  +F LY++++G  GV+DHSG S  +P++Y + DH  HH   NVNY FPF ++DL+H T+
Sbjct:   42 GYTIYSSLLHVIFYRHAPPPVRSPH----HMTIVSFNLIMAATFAMLSTELHVRGHSRMCFDD---SSPPSSLVVSQAVLAMLGSNLVEYYWHRLLHIRALYVRVHKVHHHYKRPQPFDDMYMHPFEGAIYFCILYGPPFVLPMNVHAFILYMVVMGLFGVLDHSGLSFHIPLVYKAQDHAVHHAKVNVNYGFPFVYLDLVHGTF 239          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: L1JX35_GUITC (Sterol desaturase n=2 Tax=Guillardia theta TaxID=55529 RepID=L1JX35_GUITC)

HSP 1 Score: 157 bits (398), Expect = 2.290e-44
Identity = 81/177 (45.76%), Postives = 106/177 (59.89%), Query Frame = 1
Query:   94 FHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            +H     +N+  A     F V  IVRG T L FD     A  L  V      A+ +QS+ EYYWH LMH    YK FHK HH YK P+PFDDMYIHP+E+ GYYC  ++P  V  +    F LY+ I+G CG++DHSG       +YN++DH+ HH  F+ N+AFPF +MD+LH T+
Sbjct:   72 YHDFVCAMNVITAGCSAGFVVRSIVRGSTRLLFD--WKAAGRLPGVVGTFCLAVGWQSVLEYYWHRLMHLPFFYKHFHKMHHSYKSPQPFDDMYIHPVEAVGYYCILYSPPFVFPLHVYGFVLYMAIMGVCGILDHSGIKWGFLGIYNTEDHDKHHEHFDCNFAFPFVWMDILHGTF 246          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A061RXV7_9CHLO (C-4 sterol methyl oxidase n=2 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061RXV7_9CHLO)

HSP 1 Score: 157 bits (396), Expect = 5.090e-44
Identity = 83/178 (46.63%), Postives = 111/178 (62.36%), Query Frame = 1
Query:   97 HWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFA-PAHVL-KISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            H ++  +NL  A+ F+ F  E  +RG   +   E   T + L   +L  ++A + QS+  YYWH LMH  P YK  HK HH YK PRPFDD++IHP+E+FGYY   +  PAH L ++    ++LY+ I G  GV+DHSG  + VP  Y+  DH+AHHR FNVNYAFPF  MD+ H TY
Sbjct:   80 HGLYCTINLLTASSFLAFVTEAHIRGWNNIKLPE-PDTLIYLE--WLPFLRATLAQSVLVYYWHRLMHWRPCYKALHKLHHFYKAPRPFDDLFIHPVEAFGYYTLLYLFPAHCLGRMPLGDYALYLTIHGVFGVLDHSGIYLSVPPFYHVSDHDAHHRHFNVNYAFPFVLMDIFHGTY 254          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A067CC58_SAPPC (Fatty acid hydroxylase domain-containing protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067CC58_SAPPC)

HSP 1 Score: 154 bits (389), Expect = 2.510e-43
Identity = 80/184 (43.48%), Postives = 112/184 (60.87%), Query Frame = 1
Query:   73 RAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            RA   A  H      NL VA++F     E  VRG + +TF   G +   L    L +  A VF++  EYYWH  +HT   Y R HK HH+YK PRPFDDMY+HP+E   YY   + P +V  +   SFS+Y+ ++G  G++DHSG ++++P++Y++ DHE HHR  N NY FPF ++D+LH TY
Sbjct:   51 RAQPQAPDHSALTLFNLLVASVFAMMAAEGHVRGLSRMTF---GPSPPPLSMA-LQVGLACVFENTVEYYWHRFLHTRAPYARVHKVHHYYKSPRPFDDMYMHPVEGMAYYWVLYGPPYVFAMHWQSFSVYMALMGLFGLLDHSGIAVRMPLVYDTRDHELHHRRVNANYGFPFQYLDVLHGTY 230          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: T0RTT2_SAPDV (Fatty acid hydroxylase domain-containing protein n=1 Tax=Saprolegnia diclina (strain VS20) TaxID=1156394 RepID=T0RTT2_SAPDV)

HSP 1 Score: 152 bits (384), Expect = 1.720e-42
Identity = 80/208 (38.46%), Postives = 115/208 (55.29%), Query Frame = 1
Query:    1 LFFGYILFSIGLALQHIKKGDQVVRAPKTAHFHWIFVFLNLSVAALFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            +F GY+       L+ +       R+   A  H      NL VA++F     E  +RG   L+   FG +   L  + L +  A +F++  EYYWH  +HT   Y   HK HHHYK PRPFDDMY+HP+E   YY   + P +V  +   SF +Y+ ++G  G++DHSG  +++P++Y++ DHE HHR  N NY FPF + D LH TY
Sbjct:   36 VFLGYL--GASFLLERVFYPSPSPRSQPKAPDHTALTLFNLLVASVFAMLAAEGHMRG---LSRMSFGPSPAPLT-IALEVGLACLFENTVEYYWHRFLHTRAAYAHVHKVHHHYKSPRPFDDMYMHPVEGMAYYWVLYGPPYVFAMHMHSFCIYMALMGLFGLLDHSGIPVRIPLVYDTRDHEIHHRRVNYNYGFPFQYFDFLHGTY 237          
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Match: A0A024URC2_9STRA (Fatty acid hydroxylase domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024URC2_9STRA)

HSP 1 Score: 143 bits (360), Expect = 1.910e-39
Identity = 73/180 (40.56%), Postives = 104/180 (57.78%), Query Frame = 1
Query:   97 HWIFVFLNLSVAALFMTFTVERIVRGDTFLTFD----EFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAVSFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 624
            H   V  NL +AA +     E  VRG + + FD     +   AV +    LG        +  EYYWH L+HT  +Y R HK HH+YK PRPFDDMY+HP+E   Y+   + P  V+ +  VSF +Y++++G  GV+DHSG S ++P++Y S+DH  HH   + N  FP P+ D+LH T+
Sbjct:   16 HTTLVSFNLLLAATYAMAAAELHVRGRSAMVFDAEWPSWKTIAVQVALAILG-------ANCVEYYWHRLLHTRFLYTRVHKVHHYYKRPRPFDDMYMHPVEGAAYFFILYGPPFVMSMHVVSFVIYMMVMGLFGVMDHSGLSFRIPLVYTSNDHATHHAKVHFNLGFPLPYWDILHGTH 188          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig981.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 23
Match NameE-valueIdentityDescription
A0A482SNF4_9ARCH1.180e-5044.51Fatty acid hydroxylase family protein n=1 Tax=arch... [more]
W7TYR2_9STRA1.470e-4745.26C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis ... [more]
A0A6G0WDA8_9STRA2.160e-4741.83Fatty acid hydroxylase domain-containing protein n... [more]
A0A1V9YQH7_9STRA4.530e-4541.35Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=12... [more]
A0A485KEW3_9STRA5.740e-4540.00Aste57867_4084 protein n=1 Tax=Aphanomyces stellat... [more]
L1JX35_GUITC2.290e-4445.76Sterol desaturase n=2 Tax=Guillardia theta TaxID=5... [more]
A0A061RXV7_9CHLO5.090e-4446.63C-4 sterol methyl oxidase n=2 Tax=Tetraselmis sp. ... [more]
A0A067CC58_SAPPC2.510e-4343.48Fatty acid hydroxylase domain-containing protein n... [more]
T0RTT2_SAPDV1.720e-4238.46Fatty acid hydroxylase domain-containing protein n... [more]
A0A024URC2_9STRA1.910e-3940.56Fatty acid hydroxylase domain-containing protein n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig981contigH-akashiwo_Contig981:231074..231697 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog55529.EKX53146
PFAMsFA_hydroxylase
Model size624
Max annot lvl2759|Eukaryota
Hectar predicted targeting categorysignal peptide
Exons1
Evalue3.75e-45
EggNOG OGsCOG3000@1|root,KOG0873@2759|Eukaryota
DescriptionC-4 methylsterol oxidase activity
Cds size624
COG categoryI
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388067.0312595-CDS-H-akashiwo_Contig981:231073..2316971681388067.0312595-CDS-H-akashiwo_Contig981:231073..231697Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig981 231074..231697 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig981.2.1prot_H-akashiwo_Contig981.2.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig981 231074..231697 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig981.2.1

>prot_H-akashiwo_Contig981.2.1 ID=prot_H-akashiwo_Contig981.2.1|Name=mRNA_H-akashiwo_Contig981.2.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=208bp
LFFGYILFSIGLALQHIKKGDQVVRAPKTAHFHWIFVFLNLSVAALFMTF
TVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMH
TGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCFFFAPAHVLKISAV
SFSLYVIILGTCGVIDHSGCSIKVPMLYNSDDHEAHHRFFNVNYAFPFPF
MDLLHQTY
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mRNA from alignment at H-akashiwo_Contig981:231074..231697+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig981.2.1 ID=mRNA_H-akashiwo_Contig981.2.1|Name=mRNA_H-akashiwo_Contig981.2.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=624bp|location=Sequence derived from alignment at H-akashiwo_Contig981:231074..231697+ (Heterosigma akashiwo CCMP452)
CTTTTCTTCGGCTACATCCTCTTCAGTATTGGCCTTGCTTTGCAACATAT CAAAAAGGGCGACCAGGTGGTTCGGGCCCCTAAAACTGCCCATTTCCATT GGATTTTCGTTTTTCTTAACCTCTCAGTCGCTGCACTGTTCATGACTTTC ACAGTTGAGAGAATAGTACGAGGGGACACCTTCTTGACGTTTGATGAATT TGGAATCACTGCAGTGGACTTGAGAAAAGTTTTTCTGGGTTTGGTCAAAG CTCTCGTTTTTCAGTCTATTTGTGAGTATTATTGGCATTTACTCATGCAC ACAGGACCCATGTACAAGAGGTTCCACAAATTTCATCACCACTACAAAGT TCCCAGGCCCTTTGACGATATGTACATCCACCCTATAGAGTCCTTTGGGT ACTATTGCTTTTTTTTTGCGCCAGCCCATGTTCTGAAAATTTCAGCAGTC AGCTTCAGTCTTTATGTGATAATTTTGGGGACCTGTGGTGTCATTGATCA TTCAGGATGCAGTATCAAGGTCCCTATGCTATACAATAGTGATGATCATG AAGCGCATCATCGGTTCTTCAATGTCAACTATGCATTTCCATTTCCTTTT ATGGACCTGCTGCACCAAACCTAT
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Coding sequence (CDS) from alignment at H-akashiwo_Contig981:231074..231697+

>mRNA_H-akashiwo_Contig981.2.1 ID=mRNA_H-akashiwo_Contig981.2.1|Name=mRNA_H-akashiwo_Contig981.2.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=624bp|location=Sequence derived from alignment at H-akashiwo_Contig981:231074..231697+ (Heterosigma akashiwo CCMP452)
CTTTTCTTCGGCTACATCCTCTTCAGTATTGGCCTTGCTTTGCAACATAT
CAAAAAGGGCGACCAGGTGGTTCGGGCCCCTAAAACTGCCCATTTCCATT
GGATTTTCGTTTTTCTTAACCTCTCAGTCGCTGCACTGTTCATGACTTTC
ACAGTTGAGAGAATAGTACGAGGGGACACCTTCTTGACGTTTGATGAATT
TGGAATCACTGCAGTGGACTTGAGAAAAGTTTTTCTGGGTTTGGTCAAAG
CTCTCGTTTTTCAGTCTATTTGTGAGTATTATTGGCATTTACTCATGCAC
ACAGGACCCATGTACAAGAGGTTCCACAAATTTCATCACCACTACAAAGT
TCCCAGGCCCTTTGACGATATGTACATCCACCCTATAGAGTCCTTTGGGT
ACTATTGCTTTTTTTTTGCGCCAGCCCATGTTCTGAAAATTTCAGCAGTC
AGCTTCAGTCTTTATGTGATAATTTTGGGGACCTGTGGTGTCATTGATCA
TTCAGGATGCAGTATCAAGGTCCCTATGCTATACAATAGTGATGATCATG
AAGCGCATCATCGGTTCTTCAATGTCAACTATGCATTTCCATTTCCTTTT
ATGGACCTGCTGCACCAAACCTAT
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