mRNA_H-akashiwo_Contig87.10.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig87.10.1
Unique NamemRNA_H-akashiwo_Contig87.10.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A7S0K957_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus aporus TaxID=1398097 RepID=A0A7S0K957_9STRA)

HSP 1 Score: 106 bits (264), Expect = 1.530e-24
Identity = 58/128 (45.31%), Postives = 71/128 (55.47%), Query Frame = 2
Query:  152 EESCTACQFCNDTCSLADCTTCSDKRQ---------RLRSVRKDRT--DSYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLL-EKFRIGVLIQC 499
            E +C AC FC DTC    C  C  +             R  RK RT  +  +T C+V RH T  S W+V +G +YD T +LSRHPGG  SIL   GG  D SED+ YHSK A+  L  KFRIGV++ C
Sbjct:   71 EGTCDACPFCQDTCMDPSCAICKSELVTSHSKPFFVNTRRKRKLRTVPEQCYTMCQVRRHNTRESAWLVADGVIYDATPFLSRHPGGERSILKYSGGVKDCSEDLNYHSKNARNFLWPKFRIGVVVDC 198          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A8J2SW01_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SW01_9STRA)

HSP 1 Score: 108 bits (271), Expect = 5.360e-24
Identity = 56/128 (43.75%), Postives = 80/128 (62.50%), Query Frame = 2
Query:  155 ESCTACQFCNDTCSLADCTTCSDKRQRLRSVRKDRTDSYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQCGQVVSSSDSGCSI 538
            ++C AC +C+DTC+  DC  C+  R R R +   R  + F+PCEV RH +  SCW+V    V+D T +L RHP G  SI+ R  GGAD +ED+ +HS +AQKL  + +IG L  CG    + D+GC++
Sbjct:  194 DACDACPYCHDTCADPDCPRCAVWRLRPRKM--CRGCAVFSPCEVRRHASAESCWLVAGRDVFDVTSFLPRHPAGTRSIV-RKAGGADCTEDLGFHSAKAQKLWMQHKIGRLEPCG-AEKNPDNGCAV 317          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A7S3K3K6_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K3K6_9STRA)

HSP 1 Score: 98.6 bits (244), Expect = 6.390e-22
Identity = 49/116 (42.24%), Postives = 67/116 (57.76%), Query Frame = 2
Query:  152 EESCTACQFCNDTCSLADCTTCSDKRQRLRSVRKDRTDSYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQC 499
            E+ C AC +C+D CS   C  CS K    R   +      FT CE+ RH T  SCW+V   ++YD T ++ RHP G  SI+ R  GG D +ED+ +HS +AQKL  + +IG L+ C
Sbjct:   27 EQICDACPYCDDRCSNPCCIVCSTKPITKRQGNR------FTRCEIRRHSTAESCWLVAGSTIYDVTSFIPRHPAGTYSIV-RNAGGRDCTEDLAFHSGKAQKLWNQHKIGRLMLC 135          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A7S3V462_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3V462_9STRA)

HSP 1 Score: 98.6 bits (244), Expect = 1.110e-21
Identity = 52/175 (29.71%), Postives = 88/175 (50.29%), Query Frame = 2
Query:   44 MCQSFFAIPAGDDLAHPISPDLLSVD----ESGFPVVLTAEESCTACQFCNDTCSLADCTTCSDKRQR-------------------LRSVRKDRTDSYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQC 499
            MC+S       D +   ++P L +      ++  P ++  + SC AC  C D C+   C +C +K +                     R+V+ D ++ Y TPC++ARH T  S W++    ++D T ++  HPGG  SIL + GG  D + D+++HS +A K+ ++ RIG+L  C
Sbjct:   18 MCES-----DNDTIDRNMAPQLQTASKHDSDTSIPDLVYIQSSCDACPNCPDVCASNSCLSCREKAKMDSSITERPSRNKTPSNPFLSRNVKNDESELYITPCQLARHNTKESAWLLCGYEIFDATMFIKGHPGGESSILRKAGGVVDCARDLKFHSPRAIKMWKQSRIGILRPC 187          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A7S3LGK6_9STRA (Hypothetical protein n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3LGK6_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 1.890e-20
Identity = 53/145 (36.55%), Postives = 72/145 (49.66%), Query Frame = 2
Query:  101 PDLLSVDESGFPVVLTAEESCTACQFCNDTCSLADCTTCSDKRQRLRSVR----KDRTDSYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQCGQVVSSSD 523
            PD  S D S     L     C AC +C+DTC    C  C +K     S      +  +   FT C+V +H T  S WIV    +YD T Y+ RHPGG   IL + GG  D ++D Q+HSK  ++   K+++GVL+ C    S  D
Sbjct:   34 PDGASDDSS-----LVGSGPCEACPYCSDTCGDDSCKLCVNKTCLEISTPTCPPRSASLPVFTLCQVRKHCTEKSVWIVAGKDIYDVTAYIDRHPGGRECILRKAGGARDCTDDFQFHSKAGRRGWNKYKVGVLVPCPGHASHQD 173          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: W6KYX4_9TRYP (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Phytomonas sp. isolate Hart1 TaxID=134013 RepID=W6KYX4_9TRYP)

HSP 1 Score: 91.3 bits (225), Expect = 1.180e-19
Identity = 43/73 (58.90%), Postives = 52/73 (71.23%), Query Frame = 2
Query:  272 FTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVL 490
            F+  EVA H T  S WIV+ GSVYD T YL  HPGGI +IL RGGG  D + D+ +HS +AQKLLE F+IGV+
Sbjct:   17 FSADEVACHCTRESLWIVMRGSVYDVTSYLGSHPGGIETILKRGGGAKDCAVDMSFHSSRAQKLLESFKIGVI 89          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: B8BQT3_THAPS (Cytochrome b5 heme-binding domain-containing protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BQT3_THAPS)

HSP 1 Score: 87.0 bits (214), Expect = 1.060e-18
Identity = 35/78 (44.87%), Postives = 52/78 (66.67%), Query Frame = 2
Query:  272 FTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQCGQ 505
            +T C++ RH T  S WI+V  ++YD T YL  HPGG  +IL + GG  D +ED+ +HSK+AQK   K+++G L +C +
Sbjct:    2 YTMCQLRRHNTADSAWILVGDTIYDATPYLRNHPGGTTTILKKSGGVVDCTEDLSFHSKRAQKEWRKYKVGTLRRCSR 79          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: W6KDY4_9TRYP (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Phytomonas sp. isolate EM1 TaxID=134006 RepID=W6KDY4_9TRYP)

HSP 1 Score: 89.0 bits (219), Expect = 1.440e-18
Identity = 39/69 (56.52%), Postives = 51/69 (73.91%), Query Frame = 2
Query:  284 EVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVL 490
            E+ARH T  S WIVV+ SVYD T YL  HPGGI S+L RGGG  D S D+ +H+ +A+K+LE F+IG++
Sbjct:   22 EIARHCTRESLWIVVDNSVYDVTSYLGSHPGGIESLLKRGGGARDCSADMAFHTSRARKMLESFKIGIV 90          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A6G0WP77_9STRA (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WP77_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 6.590e-18
Identity = 39/87 (44.83%), Postives = 57/87 (65.52%), Query Frame = 2
Query:  281 CEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLIQCGQVVSSSDSGCSIC 541
            CEV RH++L SCW+V NG VYD T  + +HP G   IL R  GG D ++D+++HSK AQ+  ++  IG L+ CG +  ++D G  +C
Sbjct:  119 CEVKRHKSLTSCWLVSNGIVYDVTNVIQQHPAGTKCIL-RKAGGQDCTQDLKFHSKDAQRCWKQCAIGKLLPCGDIDPAADDGPGLC 204          
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Match: A0A7S1VLX7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1VLX7_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 1.030e-17
Identity = 43/117 (36.75%), Postives = 59/117 (50.43%), Query Frame = 2
Query:  161 CTACQFCNDTCSLADCTTCSDKRQRLRSVRKDRTD------------------SYFTPCEVARHRTLGSCWIVVNGSVYDCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQ 457
            C AC  C D C+  DC +C   R        D T                   SY+TPC++ RH T+ S W++V  ++YD T YL RHPGG  SIL R GG  D  +D+ +HS++ +
Sbjct:   69 CDACPHCGDRCARVDCLSC---RPASPESETDDTSVEEVGKCGFPWGDDSSQLSYYTPCQLRRHNTMESAWLLVGDTIYDATTYLRRHPGGSTSILRRAGGAVDCGQDLAFHSRKGR 182          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig87.10.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S0K957_9STRA1.530e-2445.31Hypothetical protein n=1 Tax=Leptocylindrus aporus... [more]
A0A8J2SW01_9STRA5.360e-2443.75Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A7S3K3K6_9STRA6.390e-2242.24Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S3V462_9STRA1.110e-2129.71Hypothetical protein n=1 Tax=Chaetoceros debilis T... [more]
A0A7S3LGK6_9STRA1.890e-2036.55Hypothetical protein n=1 Tax=Amphora coffeiformis ... [more]
W6KYX4_9TRYP1.180e-1958.90Cytochrome b5 heme-binding domain-containing prote... [more]
B8BQT3_THAPS1.060e-1844.87Cytochrome b5 heme-binding domain-containing prote... [more]
W6KDY4_9TRYP1.440e-1856.52Cytochrome b5 heme-binding domain-containing prote... [more]
A0A6G0WP77_9STRA6.590e-1844.83Cytochrome b5 heme-binding domain-containing prote... [more]
A0A7S1VLX7_9STRA1.030e-1736.75Hypothetical protein (Fragment) n=1 Tax=Grammatoph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig87contigH-akashiwo_Contig87:552101..552815 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog112098.XP_008608302.1
PFAMsCyt-b5
Model size715
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons1
Evalue9.23e-21
EggNOG OGs2D10A@1|root,2SG82@2759|Eukaryota
Ec32 ortholog descriptionCytochrome b5-like heme/steroid binding domain
Ec32 orthologEc-12_006480.1
DescriptionCytochrome b5-like Heme/Steroid binding domain
Cds size501
COG categoryS
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388018.5066319-UTR-H-akashiwo_Contig87:552100..5521431681388018.5066319-UTR-H-akashiwo_Contig87:552100..552143Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig87 552101..552143 +
1681388018.5246537-UTR-H-akashiwo_Contig87:552644..5528151681388018.5246537-UTR-H-akashiwo_Contig87:552644..552815Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig87 552645..552815 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388018.5162294-CDS-H-akashiwo_Contig87:552143..5526441681388018.5162294-CDS-H-akashiwo_Contig87:552143..552644Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig87 552144..552644 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig87.10.1prot_H-akashiwo_Contig87.10.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig87 552144..552644 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig87.10.1

>prot_H-akashiwo_Contig87.10.1 ID=prot_H-akashiwo_Contig87.10.1|Name=mRNA_H-akashiwo_Contig87.10.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=167bp
MCQSFFAIPAGDDLAHPISPDLLSVDESGFPVVLTAEESCTACQFCNDTC
SLADCTTCSDKRQRLRSVRKDRTDSYFTPCEVARHRTLGSCWIVVNGSVY
DCTKYLSRHPGGIPSILSRGGGGADASEDIQYHSKQAQKLLEKFRIGVLI
QCGQVVSSSDSGCSIC*
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mRNA from alignment at H-akashiwo_Contig87:552101..552815+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig87.10.1 ID=mRNA_H-akashiwo_Contig87.10.1|Name=mRNA_H-akashiwo_Contig87.10.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=715bp|location=Sequence derived from alignment at H-akashiwo_Contig87:552101..552815+ (Heterosigma akashiwo CCMP452)
ACCCGTCATTATTTTACGAAGTTCAGAAAAAATAAAGGGAAACATGTGCC AATCATTTTTTGCAATCCCAGCAGGAGATGATCTTGCTCACCCGATCAGT CCTGACCTCCTCTCGGTTGATGAATCTGGCTTTCCTGTTGTGCTGACGGC GGAAGAGTCGTGTACTGCTTGCCAATTCTGTAATGATACCTGCAGCCTGG CTGACTGCACCACATGCTCTGACAAACGACAGCGACTTAGAAGCGTTCGT AAAGATAGAACTGATAGTTACTTCACACCCTGCGAAGTTGCGCGGCATCG GACATTGGGAAGCTGTTGGATAGTCGTGAATGGTAGTGTATATGACTGTA CCAAGTATCTGAGCCGGCATCCAGGAGGCATCCCTTCAATACTATCCCGA GGAGGGGGCGGTGCAGATGCTTCCGAAGATATTCAGTACCACTCAAAGCA GGCTCAGAAATTGCTTGAAAAGTTCAGGATAGGAGTGTTGATACAATGTG GTCAGGTGGTTTCAAGCAGTGATTCGGGCTGTTCAATTTGCTAGTGCCCA AGGTTTTGTATACATTGAAAACGTTAATGGTATTTGAGGTGTCAGAAATG CTCATGACCAATCATGAAAATAGTGTGTTGAATGGGCGATAACGGTTTTG TCTACTCACATTTTTGAACTGATTTAGGACAGAAATTCATTTTTACATCG TCATCCAAGAGGCCC
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Coding sequence (CDS) from alignment at H-akashiwo_Contig87:552101..552815+

>mRNA_H-akashiwo_Contig87.10.1 ID=mRNA_H-akashiwo_Contig87.10.1|Name=mRNA_H-akashiwo_Contig87.10.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=501bp|location=Sequence derived from alignment at H-akashiwo_Contig87:552101..552815+ (Heterosigma akashiwo CCMP452)
ATGTGCCAATCATTTTTTGCAATCCCAGCAGGAGATGATCTTGCTCACCC
GATCAGTCCTGACCTCCTCTCGGTTGATGAATCTGGCTTTCCTGTTGTGC
TGACGGCGGAAGAGTCGTGTACTGCTTGCCAATTCTGTAATGATACCTGC
AGCCTGGCTGACTGCACCACATGCTCTGACAAACGACAGCGACTTAGAAG
CGTTCGTAAAGATAGAACTGATAGTTACTTCACACCCTGCGAAGTTGCGC
GGCATCGGACATTGGGAAGCTGTTGGATAGTCGTGAATGGTAGTGTATAT
GACTGTACCAAGTATCTGAGCCGGCATCCAGGAGGCATCCCTTCAATACT
ATCCCGAGGAGGGGGCGGTGCAGATGCTTCCGAAGATATTCAGTACCACT
CAAAGCAGGCTCAGAAATTGCTTGAAAAGTTCAGGATAGGAGTGTTGATA
CAATGTGGTCAGGTGGTTTCAAGCAGTGATTCGGGCTGTTCAATTTGCTA
G
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