mRNA_H-akashiwo_Contig1221.6.4 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A2P4X4K6_9STRA (Formate--tetrahydrofolate ligase n=23 Tax=Peronosporaceae TaxID=4777 RepID=A0A2P4X4K6_9STRA)

HSP 1 Score: 902 bits (2331), Expect = 0.000e+0
Identity = 456/636 (71.70%), Postives = 519/636 (81.60%), Query Frame = 1

Query:  148 KKLDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAAC-ALPPPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            ++L PEQLLTPVPSDI IS A+ P+ I EIA++ GILPEE+  YG NKAK+SLD+ DRL +  NGNYVVVTG+ PTPLGEGKSTTTIGL QALGA L KK F+CIRQPSQGPTFG+KGGAAGGGYAQV+PM+EFNLHLTGDIHAITAANNLLAAAIDTRMFHE+ QSD+ALFRRL P  KDG + F+  M  RL KLGI K  PD LT+EE  +FARLDIDP TITW+RV+DTCDR +R V VG  P EKG++R TGFDITVASEIMAILALTTSL+DMR+RLGRIV+ MSR G PVTA+DLGVGGAL VLMKDA++PTLMQTVE TPV VHAGPFANIAHGNSSI+ADQ+ALKLAG +GFV+TE GFGADIGMEKFFNIKCR SGL P C V+V+TVRALKMHGGGP V AG PL  VY EENLE++  GCAN+  HI N  KFGV  VVA+N F TD+  E++ VK+ AL AGA AAVESNHWA GG+GAKAL  AVV +C  +      F FLYPLE  + EK   +  +IYGADG  FSEEA+ KLA Y   GYG LP+C AKT  SLS +PAAKGVPTGF V VRD+RASVGAGF+Y L GD+MT+PGLPTRPGFYDVD+D +TGK++GLF
Sbjct:   69 RQLKPEQLLTPVPSDIAISDAIAPLPITEIAQAAGILPEEVVPYGSNKAKISLDVRDRLKNQPNGNYVVVTGITPTPLGEGKSTTTIGLCQALGAHLNKKAFACIRQPSQGPTFGVKGGAAGGGYAQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRMFHENAQSDKALFRRLCPTSKDGSRKFSPVMFKRLQKLGITKVSPDDLTDEEIHKFARLDIDPDTITWQRVLDTCDRFLRQVVVGAGPAEKGQTRTTGFDITVASEIMAILALTTSLQDMRERLGRIVIGMSRDGEPVTADDLGVGGALAVLMKDAILPTLMQTVEGTPVFVHAGPFANIAHGNSSIIADQIALKLAGEDGFVVTECGFGADIGMEKFFNIKCRTSGLTPQCVVLVSTVRALKMHGGGPNVVAGKPLDAVYVEENLEMLERGCANMQHHIRNALKFGVAVVVAVNVFATDSPREVELVKQKALEAGATAAVESNHWAEGGKGAKALGHAVVTSCDKMRSQGSPFKFLYPLEFSIAEKFEVICKEIYGADGVEFSEEAKKKLAVYSQRGYGKLPVCCAKTHLSLSTNPAAKGVPTGFSVTVRDIRASVGAGFVYLLCGDMMTVPGLPTRPGFYDVDLDTETGKVIGLF 704          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A7S3GN13_9STRA (Formate--tetrahydrofolate ligase n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GN13_9STRA)

HSP 1 Score: 897 bits (2317), Expect = 0.000e+0
Identity = 454/631 (71.95%), Postives = 518/631 (82.09%), Query Frame = 1

Query:  178 PVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQ------GFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            PVPSDI++SQ++TP  IGE+AE+ GILP E  LYG NK K+SLD+ +RL    +GNYV+VTG+NPTPLGEGKSTTTIGL+QALGA L KK  +CIRQPSQGPTFGIKGGAAGGGYAQV+PMEEFNLHLTGDIHAI AANNLLAAAI+TRMFHES QSD++LF RL PA KDG + F+  ML RLAKLGIDKTDP+  T+EE+ RFARLDIDP TITW RV+D CDR +RG+  G    E    R TGFDITVASEIMA+LALTTSL DMRDRLGR+V+ MS+AG PVTA+DLGVGGALTVLMKDA+MPT MQTVE+TPV VHAGPFANIAHGNSS++ADQ+ALK+ GP+GF +TEAGFGADIGMEKFFNIKCRYSGL P CAVIVATVRALKMHGGGP V AG PL  VYKEE + LV AGCANL  HI+N +KFGV AVVAIN+F TDT AE++ V+ L+LAAGA AAV +NHWA GG GA  L  AV+ ACA    A       GF FLYPLE+ L+EKI  +  +IYGADG  FSE AE ++ +Y   GYG LPIC+AKTQYSLS D AAKGVPTGF V VRDVRA+VGAG++Y + GDIMTIPGLPTRPGFYDVDID  TG+++GLF
Sbjct:   19 PVPSDIEVSQSITPQPIGEVAEAVGILPSEYFLYGSNKCKISLDIRERLGSNQDGNYVLVTGINPTPLGEGKSTTTIGLAQALGAHLNKKAVACIRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAIGAANNLLAAAIETRMFHESQQSDKSLFSRLCPAEKDGSRKFSPVMLRRLAKLGIDKTDPNEFTDEEKARFARLDIDPDTITWNRVLDVCDRFLRGIVTGAGKNEHFE-RNTGFDITVASEIMAVLALTTSLADMRDRLGRMVIGMSKAGEPVTADDLGVGGALTVLMKDAIMPTCMQTVEQTPVFVHAGPFANIAHGNSSVIADQIALKVVGPDGFCVTEAGFGADIGMEKFFNIKCRYSGLTPQCAVIVATVRALKMHGGGPDVVAGKPLDHVYKEEAVGLVEAGCANLVHHIKNIQKFGVSAVVAINRFSTDTDAELEMVRTLSLAAGATAAVVANHWAEGGAGAADLGRAVIDACAAARAAAETDASTGFKFLYPLEMPLREKIATICREIYGADGVDFSEAAERRIESYTSVGYGSLPICMAKTQYSLSTDAAAKGVPTGFRVLVRDVRAAVGAGYIYPICGDIMTIPGLPTRPGFYDVDIDTTTGRVIGLF 648          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A3M6V8G8_9STRA (Formate--tetrahydrofolate ligase n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6V8G8_9STRA)

HSP 1 Score: 891 bits (2303), Expect = 0.000e+0
Identity = 454/648 (70.06%), Postives = 519/648 (80.09%), Query Frame = 1

Query:  112 LRHSSTNQKMAAKKLDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACA-LPPPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            L+ + T      ++L  EQLLTPVPSDI IS AMTP+ I EIA++ GILPEE+  YG  KAK+SL + DRL D  NGNYVVVTG+ PTPLGEGKSTTTIGL QALGA L KK F+CIRQPSQGPTFG+KGGAAGGGYAQV+PM+EFNLHLTGDIHAITAANNLLAAAIDTRMFHE+ QSD+ALFRRL P  KDG + F+  ML RL KLGI K  PD LT+EE  +FARLDIDP TITW+RV+DTCDR +R V VG  P EKG++R+TGFDITVASEIMAILALTTSL+DMR+RLGRIV+ MSR G PVTA+DLGVGGAL VLMKDA++PTLMQTVE TPV VHAGPFANIAHGNSSI+ADQ+ALKLAG +GFV+TE GFGADIGMEKFFNIKCR SGL P C V+V+T+RALKMHGGGP V AG  L  VY +ENLE++  GCAN+  HI N  KFGV  VVA+N FVTD+  EI+ VK  AL AGAAAAVESNHW+ GG+GAK L  AV+ +C  +      F FLYPLE  + EK   +  +IYGAD   FSEEA+ KLA Y   GYG LP+C AKT  SLS DP AKGVPTGF V VRD+RASVGAGF+Y L GD+ T+PGLPTRPGFYDVD+D +TGK++GLF
Sbjct:   32 LQSTLTPSAKMPRQLKSEQLLTPVPSDITISDAMTPLPITEIAQAAGILPEEVIPYGSTKAKISLSVRDRLKDQPNGNYVVVTGITPTPLGEGKSTTTIGLCQALGAHLDKKAFACIRQPSQGPTFGVKGGAAGGGYAQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRMFHENAQSDKALFRRLCPTAKDGFRKFSPVMLKRLQKLGITKLSPDDLTDEEIRKFARLDIDPDTITWQRVLDTCDRFLRQVVVGAGPAEKGQTRKTGFDITVASEIMAILALTTSLQDMRERLGRIVIGMSRGGEPVTADDLGVGGALAVLMKDAILPTLMQTVEGTPVFVHAGPFANIAHGNSSIIADQIALKLAGEDGFVVTECGFGADIGMEKFFNIKCRTSGLTPQCVVLVSTIRALKMHGGGPNVVAGKQLDPVYVDENLEMLERGCANMQHHIRNALKFGVAVVVAVNVFVTDSPREIELVKHKALEAGAAAAVESNHWSEGGKGAKNLGRAVITSCKKMRTQGSQFKFLYPLEFTIAEKFEVICKEIYGADSVEFSEEAKMKLAVYSERGYGNLPVCCAKTHLSLSTDPTAKGVPTGFSVTVRDIRASVGAGFVYLLCGDMTTVPGLPTRPGFYDVDLDTETGKVIGLF 679          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A6G0WBW9_9STRA (Formate--tetrahydrofolate ligase n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WBW9_9STRA)

HSP 1 Score: 885 bits (2286), Expect = 0.000e+0
Identity = 444/633 (70.14%), Postives = 514/633 (81.20%), Query Frame = 1

Query:  154 LDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            L P  LL PVPSDI++S ++TP HIG+IA++ G+L +EL  +GK KAKV L + DRL DV +G+YVVVTG+ PTPLGEGKSTTTIGL+QALGA L  K F+CIRQPSQGPTFGIKGGAAGGGYAQVVPM+EFNLH+TGDIHAITAANNL+AAAID RMFHESTQ D+ALF RL P  KDG + FA  ML RLAKLGIDKT+P+ LTE+E  RFARLDIDP TITW RV+DTCDR +RGVT+GQA TE G SR+TGFDITVASEIMA+LAL TSL DMR+RLGR+V+ MSRAG PVT +DLGVGGALT LMKDA+MPTLMQTVE+TPV+VHAGPFANIAHGNSSI+ADQ+ALKL GPEG V+TEAGFGADIGMEKFF+IKCR SGLVP C V+VATVRALKMHGGGP V AG PL  VY +EN++LV  GC N+ +HI N  KFGV  VV++N+F TDT  EI+ V+  AL AGA+AA+E NHWA GG GA  L +AV+ ACA   P+  F FLYPLE  +++K+  V   IYGAD   F+  A+ K+  +   GY   PIC+AKT  SLS DP+ K VPTGF V +RD+RASVGAGFLY L GDIMT+PGLPTRPGFYDVD+D  TGKIVGLF
Sbjct:    4 LPPSALLFPVPSDIEVSDSITPAHIGDIAKASGLLEDELVYFGKTKAKVQLSVRDRLQDVKDGSYVVVTGITPTPLGEGKSTTTIGLAQALGAHLKTKSFACIRQPSQGPTFGIKGGAAGGGYAQVVPMDEFNLHMTGDIHAITAANNLVAAAIDARMFHESTQKDKALFNRLCPVKKDGSRVFASVMLRRLAKLGIDKTNPNDLTEDEIRRFARLDIDPATITWNRVLDTCDRFLRGVTIGQAATEAGHSRDTGFDITVASEIMAVLALATSLEDMRERLGRMVIGMSRAGNPVTTDDLGVGGALTALMKDAIMPTLMQTVEQTPVLVHAGPFANIAHGNSSIIADQIALKLVGPEGIVVTEAGFGADIGMEKFFDIKCRASGLVPQCVVLVATVRALKMHGGGPTVTAGKPLDPVYVDENIDLVTVGCENMMQHIRNAVKFGVKVVVSVNRFATDTDREIEIVRAKALEAGASAAIECNHWAKGGAGAVELGQAVIDACA-SVPSNAFKFLYPLEWSIEQKVEAVCKSIYGADSVEFTPLAQEKIRVFTANGYDKFPICMAKTHLSLSTDPSKKNVPTGFSVTIRDIRASVGAGFLYLLCGDIMTVPGLPTRPGFYDVDVDCKTGKIVGLF 635          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: T0RF22_SAPDV (Formate--tetrahydrofolate ligase n=3 Tax=Saprolegniaceae TaxID=4764 RepID=T0RF22_SAPDV)

HSP 1 Score: 885 bits (2286), Expect = 0.000e+0
Identity = 451/633 (71.25%), Postives = 510/633 (80.57%), Query Frame = 1

Query:  154 LDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            L P QL TPVPSDI+IS A TPVHI EIA + G+L +E+  YGK KAKV+L + DRLS V +G YVVVTG+ PTPLGEGKSTTTIGL+QALGA L KK F+CIRQPSQGPTFGIKGGAAGGGYAQVVPM+EFNLH+TGDIHAITAANNLL+AAID RMFHESTQ D+ALF RL PA KDG + FA  ML RL KLGI KT+P  LT++E  RFARLDIDP T+TW RV+DTCDR +RG+T+G+APTEKG  RETGFDITVASEIMA+LAL TSL DMR+RLGR+VV MSR G PVTA+DLGVGGALT LMKDA+MPTLMQTVE+TPV+VHAGPFANIAHGNSSI+ADQ+ALKL G +G V+TEAGFGADIGMEKFF+IKCRYSGL P C V+VATVRALKMHGGGP V AG PL  VY +ENL LV AGC N+ +HI N RKFGV  VV++N+F TD+  EI  V   A+ AGA AAVE NHWALGG GA  LAEAVV ACA   P   F FLYPLE  ++EK   V   IYGAD   FS+ A+AKL  +   GY   PIC+AKT  S S DP  K VPTGF V +RD+RASVGAGFLY L GDIMT+PGLPTRPGFYD+D+D  +GKI+GLF
Sbjct:    4 LTPAQLKTPVPSDIEISDATTPVHIREIATAAGLLEDEIYFYGKTKAKVALAVRDRLSHVEHGAYVVVTGITPTPLGEGKSTTTIGLAQALGAHLKKKSFACIRQPSQGPTFGIKGGAAGGGYAQVVPMDEFNLHMTGDIHAITAANNLLSAAIDARMFHESTQKDKALFNRLCPAKKDGSRVFAPVMLRRLRKLGITKTNPSELTDDEISRFARLDIDPATLTWNRVLDTCDRFLRGITIGKAPTEKGHERETGFDITVASEIMAVLALATSLDDMRERLGRMVVGMSRKGDPVTADDLGVGGALTALMKDAIMPTLMQTVEQTPVLVHAGPFANIAHGNSSIIADQIALKLVGRDGVVVTEAGFGADIGMEKFFDIKCRYSGLRPQCVVLVATVRALKMHGGGPTVTAGKPLDAVYVDENLALVTAGCDNMMQHIRNARKFGVSVVVSVNRFATDSDKEIAIVCAKAVEAGALAAVECNHWALGGRGAVELAEAVVKACA--SPTNNFKFLYPLEASIEEKARAVCQKIYGADDVIFSDLAKAKLEVFTKCGYSHFPICMAKTHLSFSTDPTKKNVPTGFSVTIRDIRASVGAGFLYLLCGDIMTVPGLPTRPGFYDIDVDTTSGKIIGLF 634          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A8K1FBV6_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FBV6_PYTOL)

HSP 1 Score: 884 bits (2283), Expect = 0.000e+0
Identity = 449/635 (70.71%), Postives = 516/635 (81.26%), Query Frame = 1

Query:  154 LDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQG--FSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            L  +QLL PVPSDI IS A+ P+ I EIA + GILPEE+  YG +KAK+SLD+ DRL D  NGNYVVVTG+ PTPLGEGKSTTTIGL QALGA L +KVF+CIRQPSQGPTFG+KGGAAGGGYAQV+PM+EFNLHLTGDIHAITAANNLLAAAI+TRMFHESTQ D+ALF RL P  KDG + F+  ML RLAKLGI+KT+PD LTEEE  +FARLDIDP TITW+RV+DTCDR +R V VG A TEKG  R TGFDITVASEIMAILALTTSL+DMR+RLGRIV+ + + G PVTA+DLGVGGAL VLMKDA+MPTLMQTVE TPV VHAGPFANIAHGNSSI+ADQ+ALKLAG +G+V+TEAGFGADIGMEKFFNIKCR SGL P C V+V+T+RALKMHGGGP V AG PL  VY +ENLEL+  GCAN+  HI N  KFGV  VVA+N F +DT+ EI+ VK  AL AGA AAVES+HWA GG+GA  L +AVV +C     AQG  F FLYPLE  + +K   +  +IYGA    FSE+A+ K+  Y+  GYG LP+C AKT  SLS DP AKGVPTGF V VRD+RASVGAGF+Y L GD+MT+PGLPTRPGFYDVD+D +TGK++GLF
Sbjct:    5 LHSQQLLRPVPSDIAISDAIAPLLIHEIAAAAGILPEEVVPYGASKAKISLDVRDRLKDAKNGNYVVVTGITPTPLGEGKSTTTIGLCQALGAHLDRKVFACIRQPSQGPTFGVKGGAAGGGYAQVIPMDEFNLHLTGDIHAITAANNLLAAAIETRMFHESTQKDKALFNRLCPRAKDGSRTFSPVMLRRLAKLGINKTNPDDLTEEEAAKFARLDIDPETITWQRVLDTCDRFLRQVNVGVAATEKGHIRTTGFDITVASEIMAILALTTSLKDMRERLGRIVIGLDKKGEPVTADDLGVGGALAVLMKDAIMPTLMQTVEGTPVFVHAGPFANIAHGNSSIIADQIALKLAGEDGYVVTEAGFGADIGMEKFFNIKCRTSGLTPQCVVLVSTIRALKMHGGGPNVVAGQPLDPVYVDENLELLERGCANMQHHIRNALKFGVAVVVAVNVFASDTEREIELVKAKALEAGATAAVESSHWADGGKGAAKLGQAVVESCKTMR-AQGSPFKFLYPLEFTIAQKFEAICKEIYGAAAVDFSEQAKKKMEIYQARGYGSLPVCCAKTHLSLSTDPTAKGVPTGFTVTVRDIRASVGAGFVYLLCGDMMTVPGLPTRPGFYDVDLDVETGKVIGLF 638          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: K3WGN0_GLOUD (Formate--tetrahydrofolate ligase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WGN0_GLOUD)

HSP 1 Score: 875 bits (2261), Expect = 9.770e-314
Identity = 449/640 (70.16%), Postives = 511/640 (79.84%), Query Frame = 1

Query:  139 MAAKKLDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQG--FSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            M+A+ L PEQLLTPVPSDI IS A+TP+ I +IA++ GILP E+  YG  KAK+SL + DRL D  NGNYVVVTG+ PTPLGEGKSTTTIG+ QALGA L KK F+CIRQPSQGPTFG+KGGAAGGGYAQVVPM+EFNLHLTGDIHAITAANNLLAAAIDTRMFHES+Q D           KDG + F+  ML RL  LGI K  PD LT+EE  +FARLDIDP TITW+RV+D CDR +R V VG APTEKG++R TGFDITVASEIMAILALTTSL+DMR+RLGRIV+ + R G PVTA+DLGVGGAL VLMKDA+ PTLMQTVE TPV VHAGPFANIAHGNSSI+AD++ALKLAG +G+VITEAGFGADIGMEKFFNIKCR SGL P C V+V+T+RALKMHGGGP V AG PL  VY +ENLEL+  GCAN+  HI N  KFGV  VVA+N F TD++ EI+ VK  AL AGA AAVESNHWALGG+GA  L  AV+ +C     AQG  F FLYPLE+G+ EK   +A  IYGADG  FSEEA+ KL  Y   GY  LP+C AKT  SLS DP AKGVPTGF V VRD+RASVGAGF+Y L GD+MT+PGLPTRPGFYDVD+D +TGK++GLF
Sbjct:    1 MSARPLKPEQLLTPVPSDITISDAITPLPITQIADAAGILPSEVIPYGATKAKISLSVRDRLKDTPNGNYVVVTGITPTPLGEGKSTTTIGIVQALGAHLNKKAFACIRQPSQGPTFGVKGGAAGGGYAQVVPMDEFNLHLTGDIHAITAANNLLAAAIDTRMFHESSQKD-----------KDGSRKFSPVMLTRLKNLGITKITPDDLTDEEISKFARLDIDPDTITWQRVLDVCDRFLRQVVVGVAPTEKGKTRTTGFDITVASEIMAILALTTSLKDMRERLGRIVIGLDRKGEPVTADDLGVGGALAVLMKDAIQPTLMQTVEGTPVFVHAGPFANIAHGNSSIIADEIALKLAGEDGYVITEAGFGADIGMEKFFNIKCRTSGLTPQCVVLVSTIRALKMHGGGPNVVAGQPLDPVYVDENLELLERGCANMQHHIHNALKFGVAVVVAVNVFATDSEREIELVKAKALEAGATAAVESNHWALGGKGAVDLGRAVIESCG-KMRAQGSPFKFLYPLEVGIAEKFEIIAKQIYGADGVEFSEEAKKKLEIYTARGYSNLPVCCAKTHLSLSTDPTAKGVPTGFSVTVRDIRASVGAGFVYLLCGDMMTVPGLPTRPGFYDVDLDVETGKVIGLF 628          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A024TI45_9STRA (Formate--tetrahydrofolate ligase n=2 Tax=Aphanomyces TaxID=100860 RepID=A0A024TI45_9STRA)

HSP 1 Score: 865 bits (2236), Expect = 7.690e-310
Identity = 435/633 (68.72%), Postives = 504/633 (79.62%), Query Frame = 1

Query:  154 LDPEQLLTPVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            L P  L  PVPSDI++S + TP HIG+IA++ G+L +E+  YG  KAKV L + DRL+ V +G YVVVTG+ PTPLGEGKSTTTIGL+QALGA L KK F+CIRQPSQGPTFGIKGGAAGGGYAQVVPM+EFNLH+TGDIHAITAANNL+AAAID RMFHESTQSD+ALF RL P  KDG + FA  ML RL KLGIDKT+PD LT++ER  FARLDID +TITW RV+DTCDR +RG+T GQA TE G  R TGFDITVASEIMA+LAL TSL DMR+RLGR+V+ MSR+  PVTA+DLGVGGALT LMKDA+MPTLMQTVE+TPV+VHAGPFANIAHGNSSI+ADQ+ALKL G +G V+TEAGFGADIGMEKFF+IKCR SGL+P C V+VATVRALKMHGGGP V AG PL  VY +EN++LV  GCAN+ +HI N  KFGV  VV++N+F TDT  EI  V+  A+ AGA+AA+E NHW+ GG GA  L +AVV ACA P  +  F FLYPL   ++EK+  V   IYGAD   FS  A  K+  +   GY   PIC+AKT  S S DP  K VP+GF V +RD+RASVGAGFLY L GDIMT+PGLPTRPGFYDVD+D DTGKIVGLF
Sbjct:    4 LPPSALKWPVPSDIEVSDSTTPAHIGDIAKAAGLLDDEIVYYGTTKAKVQLSVRDRLASVKDGKYVVVTGITPTPLGEGKSTTTIGLAQALGAHLNKKSFACIRQPSQGPTFGIKGGAAGGGYAQVVPMDEFNLHMTGDIHAITAANNLVAAAIDARMFHESTQSDKALFNRLCPVKKDGSRVFANVMLRRLRKLGIDKTNPDDLTDDERKHFARLDIDSSTITWNRVLDTCDRFLRGITTGQAATELGHGRNTGFDITVASEIMAVLALATSLADMRERLGRMVIGMSRSEVPVTADDLGVGGALTALMKDAIMPTLMQTVEQTPVLVHAGPFANIAHGNSSIIADQIALKLVGSDGIVVTEAGFGADIGMEKFFDIKCRASGLIPQCVVLVATVRALKMHGGGPTVTAGKPLDAVYVDENIDLVTEGCANMMQHIRNALKFGVSVVVSVNRFATDTDREIQIVRSKAVEAGASAAIECNHWSKGGAGAVELGKAVVDACAAP--SNHFDFLYPLNWSIEEKVDAVCKKIYGADSVEFSPLALEKVKVFTACGYDKFPICMAKTHLSFSTDPTKKNVPSGFSVTIRDIRASVGAGFLYLLCGDIMTVPGLPTRPGFYDVDVDCDTGKIVGLF 634          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A7S2E0F1_9STRA (Formate--tetrahydrofolate ligase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2E0F1_9STRA)

HSP 1 Score: 865 bits (2236), Expect = 1.330e-309
Identity = 436/631 (69.10%), Postives = 511/631 (80.98%), Query Frame = 1

Query:  178 PVPSDIDISQAMTPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQVVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPAGKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTEKGRS----RETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALPPPAQ--GFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            PVPSDID+SQ++TP+ +G++AE+ GILP EL LYG +KAKVSLD+  RL+D  NG+YVVVTG+NPTPLGEGKSTTTIGL Q LG  LGKK  +CIRQPSQGPTFG+KGGAAGGGYAQVVPMEEFNLHLTGDIHAI A+NNLLAAAIDTR+FHE+TQSD ALFRRL P  KDG + FA PML RL +LGIDKTDP  LTEEE  RFARLDIDP TITW+RV+DTCDR +RG+ +G  P E  ++    R TG DI VASEIMA+LALTTSL DM+DRL R+V+  S++G PVT +D+G  GA+ VLMKDA+ PTLMQTVE+TPV+VHAGPFANIAHG SS++ADQ+ALKL G +GFVITEAGFGADIGMEKFFNIKCRYSGLVP CAVIVATVRALKMHGGGP V+ G  L  VY +E L+LV+AGCANL  HI N R++ V  VVA+N+F +DT AEI+ VK  ++ AGA AAV +NHWA GG GA  LA AVV AC      +   F  LYPLEL +KEKI K+  D YGADGA FS+ A  ++AAYE  G+G LPIC+AKTQYSLS DPAAKGVPTGF + VR+V+ + GAGFL  + GD+M IPGLPTRPGFYDVD+D +TG+++GLF
Sbjct:   19 PVPSDIDVSQSITPLPMGQVAEAVGILPSELLLYGAHKAKVSLDVRRRLADQKNGHYVVVTGINPTPLGEGKSTTTIGLCQGLGVALGKKNIACIRQPSQGPTFGVKGGAAGGGYAQVVPMEEFNLHLTGDIHAIVASNNLLAAAIDTRIFHEATQSDAALFRRLCPTAKDGSRKFAPPMLRRLVRLGIDKTDPSDLTEEEASRFARLDIDPDTITWRRVLDTCDRMLRGMQIGIGPAEMKKATPATRTTGVDIAVASEIMAVLALTTSLEDMKDRLCRMVIGRSKSGTPVTCDDIGCTGAIMVLMKDAVEPTLMQTVEQTPVLVHAGPFANIAHGQSSVIADQIALKLVGEDGFVITEAGFGADIGMEKFFNIKCRYSGLVPGCAVIVATVRALKMHGGGPPVKGGGELDPVYSQEALDLVQAGCANLQHHIRNARRYNVKVVVAVNKFFSDTDAEIELVKAKSMEAGAHAAVMANHWAEGGRGAAELARAVVEACESKRDKEEGAFQLLYPLELTVKEKITKICQDTYGADGAEFSDFASEQIAAYEATGFGHLPICMAKTQYSLSTDPAAKGVPTGFTIRVREVKCAAGAGFLVPICGDMMMIPGLPTRPGFYDVDLDTETGRVIGLF 649          

BLAST of mRNA_H-akashiwo_Contig1221.6.4 vs. uniprot
Match: A0A6H5KVA0_9PHAE (Formate--tetrahydrofolate ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KVA0_9PHAE)

HSP 1 Score: 863 bits (2230), Expect = 9.390e-309
Identity = 455/645 (70.54%), Postives = 524/645 (81.24%), Query Frame = 1

Query:  151 KLDPEQLLTPVPSDIDISQAM--TPVHIGEIAESCGILPEELELYGKNKAKVSLDLLDRLSDVGNGNYVVVTGVNPTPLGEGKSTTTIGLSQALGAVLGKKVFSCIRQPSQGPTFGIKGGAAGGGYAQ---VVPMEEFNLHLTGDIHAITAANNLLAAAIDTRMFHESTQSDEALFRRLVPA-GKDGKKAFAKPMLARLAKLGIDKTDPDALTEEERGRFARLDIDPTTITWKRVIDTCDRHVRGVTVGQAPTE---KGRSRETGFDITVASEIMAILALTTSLRDMRDRLGRIVVAMSRAGAPVTAEDLGVGGALTVLMKDALMPTLMQTVERTPVMVHAGPFANIAHGNSSIVADQVALKLAGPEGFVITEAGFGADIGMEKFFNIKCRYSGLVPNCAVIVATVRALKMHGGGPKVQAGTPLPQVYKEENLELVRAGCANLAKHIENTRKFGVHAVVAINQFVTDTQAEIDAVKELALAAGAAAAVESNHWALGGEGAKALAEAVVAACALP--PPAQGFSFLYPLELGLKEKIIKVATDIYGADGATFSEEAEAKLAAYEGAGYGGLPICIAKTQYSLSCDPAAKGVPTGFEVHVRDVRASVGAGFLYALAGDIMTIPGLPTRPGFYDVDIDFDTGKIVGLF 2052
            KL  +Q+ +PVP DI++SQ++  +  HI ++AE CGIL  ELE YG+ KAKVSLD+  RL    NGNYVVVTG+NPTPLGEGKSTTT+GL+QALGA LGKKVF+ IRQPSQGPTFGIKGGAAGGG +Q     PMEEFNLHLTGDIHAITAANNL+AAAIDTRMFHE+TQSDEALFRRL P  G++  + FA  ML+RL+KLGIDKT P+ LT+EE  RFARLDIDP++ITWKRV+DTCDR +R +TVGQ P E   +G  RETGFDITVASEIMA+LAL TSL+D+RDRLGR+V+  SR G  VT +DLGV GAL VLMKDA+ PTLMQT E+TPV+VHAGPFANIAHGNSSIVAD+VALKL G +GFV+TEAGFGADIGMEKFFNIKCR SG  P CAV+VATVRALKMHGGGP V+AGTPL  VYKEENL+L+R GCANLA HI N  K+GV  VV +N F TDT AEID V+ELAL AGA +AV+S+HWA GG GA  LAEAV+ ACA     P++ F FLYP E  +KEK+  V  ++Y A    +S EAE K+A YE AGYG LPIC+AKTQYSLS DP+AKG P+G  V VRDVRASVGAGF+Y L GDI TIPGLPTRPGFYDVDIDF TG++VGLF
Sbjct:    3 KLTSDQVSSPVPRDIEVSQSLEGSLAHISKVAEDCGILESELEPYGRFKAKVSLDVCRRLESSPNGNYVVVTGINPTPLGEGKSTTTVGLTQALGAHLGKKVFANIRQPSQGPTFGIKGGAAGGGDSQGGTPFPMEEFNLHLTGDIHAITAANNLVAAAIDTRMFHENTQSDEALFRRLCPENGRE--RRFAPVMLSRLSKLGIDKTRPEDLTDEEISRFARLDIDPSSITWKRVVDTCDRMLRSITVGQGPKEGESRGAVRETGFDITVASEIMAVLALATSLQDLRDRLGRMVIGRSRRGEAVTTDDLGVSGALCVLMKDAIKPTLMQTAEQTPVLVHAGPFANIAHGNSSIVADRVALKLVGEDGFVVTEAGFGADIGMEKFFNIKCRASGNTPQCAVLVATVRALKMHGGGPPVKAGTPLANVYKEENLDLLRRGCANLAVHITNALKYGVKVVVGVNVFSTDTPAEIDLVRELALEAGAFSAVKSSHWAEGGAGAVQLAEAVMGACASTRADPSKPFRFLYPSEASIKEKMNTVCREMYRAASVEWSPEAEEKVAIYEKAGYGKLPICVAKTQYSLSTDPSAKGAPSGHTVFVRDVRASVGAGFVYLLCGDINTIPGLPTRPGFYDVDIDFGTGRVVGLF 645