prot_H-akashiwo_Contig9.85.1 (polypeptide) Heterosigma akashiwo CCMP452

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_H-akashiwo_Contig9.85.1
Unique Nameprot_H-akashiwo_Contig9.85.1
Typepolypeptide
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Sequence length305
Homology
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A7S3XXY9_HETAK (Proline--tRNA ligase n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XXY9_HETAK)

HSP 1 Score: 140 bits (352), Expect = 1.430e-33
Identity = 72/99 (72.73%), Postives = 81/99 (81.82%), Query Frame = 0
Query:  206 ELPALLPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPLA 304
            ELPALLPALLEE+QAAMFAKA+AARD S+V+VTEW DFVPAL RG++VLTPFVDAGDWE+ V      E + G  E    +TSVAAKTLCIPFDQPPLA
Sbjct:  604 ELPALLPALLEEVQAAMFAKARAARDESIVRVTEWSDFVPALERGSMVLTPFVDAGDWEEVVKERSRAEALEGQPEDARTSTSVAAKTLCIPFDQPPLA 702          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A7S2UX41_9STRA (Proline--tRNA ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UX41_9STRA)

HSP 1 Score: 111 bits (278), Expect = 4.850e-26
Identity = 58/94 (61.70%), Postives = 67/94 (71.28%), Query Frame = 0
Query:  211 LPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPLA 304
            LPALLE IQA M A+AK ARD  +VQVT+W+DFVPALN+G + LTPF +   WED V      E + G  E    +TSVAAKTLCIPFDQPPLA
Sbjct:   88 LPALLETIQADMLARAKQARDDKIVQVTKWEDFVPALNKGYMALTPFCNEDPWEDTVKEKSREEALDGEAEDERTSTSVAAKTLCIPFDQPPLA 181          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A482UP51_9ARCH (Proline--tRNA ligase n=1 Tax=archaeon TaxID=1906665 RepID=A0A482UP51_9ARCH)

HSP 1 Score: 102 bits (255), Expect = 4.700e-23
Identity = 53/93 (56.99%), Postives = 63/93 (67.74%), Query Frame = 0
Query:  211 LPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            +PALL++IQ  MF +AK  RD  LV VT W+DFVPAL R  LVLTPF     WE+ V +    E + GG E+   A SVAAKTLC+PFDQPPL
Sbjct:   55 VPALLDQIQKDMFQRAKVGRDEKLVTVTRWEDFVPALERQCLVLTPFCCERSWEEAVKKRSREEALRGGEESATCAASVAAKTLCMPFDQPPL 147          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A7S0T0X0_9STRA (Proline--tRNA ligase n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0T0X0_9STRA)

HSP 1 Score: 105 bits (262), Expect = 3.820e-22
Identity = 53/90 (58.89%), Postives = 65/90 (72.22%), Query Frame = 0
Query:  214 LLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            LL++IQ  MF KAK  RD+ +V VT+W+DFVPAL + NLVLTPF D  +WE+KV ++   E + G  E    ATSVAAKTLC PFDQPPL
Sbjct:  297 LLDQIQNDMFIKAKTERDSHIVTVTKWEDFVPALEKQNLVLTPFCDITEWEEKVKKMSRDEALRGQAEAANTATSVAAKTLCKPFDQPPL 386          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: W7TR23_9STRA (Proline--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TR23_9STRA)

HSP 1 Score: 102 bits (254), Expect = 1.460e-20
Identity = 53/90 (58.89%), Postives = 63/90 (70.00%), Query Frame = 0
Query:  214 LLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            +LEEIQA +FAK KA RD  LV+V EWKDFVPAL    ++LTPFV+  +WE++V R    E + G  E    ATS AAKTLCIPFDQP L
Sbjct:  629 VLEEIQANLFAKCKAERDKKLVKVWEWKDFVPALEEKCVILTPFVNETEWEEEVKRRSREEALAGESEDARTATSAAAKTLCIPFDQPDL 718          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A835YT63_9STRA (Proline--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YT63_9STRA)

HSP 1 Score: 100 bits (249), Expect = 7.110e-20
Identity = 50/90 (55.56%), Postives = 64/90 (71.11%), Query Frame = 0
Query:  214 LLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            +LE+IQ  +FAKA A+RD    +V EW DFVPALN+ ++VLTPF + G+WE+KV  +   E + GG E    +TSVAAKTLCIP DQP L
Sbjct:  799 VLEDIQNNLFAKATASRDEHYSRVLEWADFVPALNKNDIVLTPFCNEGEWEEKVKAMSRDEALQGGSEDVRTSTSVAAKTLCIPLDQPEL 888          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A7S3H5B0_9STRA (Proline--tRNA ligase n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H5B0_9STRA)

HSP 1 Score: 100 bits (248), Expect = 8.660e-20
Identity = 51/91 (56.04%), Postives = 65/91 (71.43%), Query Frame = 0
Query:  213 ALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            ALLE IQ ++ A A+  RD  +V VT+W+DFVPAL +  LV+TPF D  +WE+KV  +   EG+ G +E+   ATSVAAKTLC PFDQPPL
Sbjct:  588 ALLETIQDSLLAAARRGRDEKMVTVTKWEDFVPALEKQCLVMTPFCDDREWEEKVKVMSREEGLKGQQESATVATSVAAKTLCKPFDQPPL 678          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: F0YK75_AURAN (Proline--tRNA ligase n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YK75_AURAN)

HSP 1 Score: 97.8 bits (242), Expect = 4.620e-19
Identity = 58/103 (56.31%), Postives = 69/103 (66.99%), Query Frame = 0
Query:  207 LPALLPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFVDAGDWE------DKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            L A L A L+++ AAMFAKAKAARDA LV+VT+W DFVP LN  NL+LTP+ D  D +      DK SR    E +G   E    ATS+AAKTLC+P DQPPL
Sbjct:  455 LGAALAAELDDVHAAMFAKAKAARDAKLVRVTDWADFVPKLNENNLILTPWCDPADQDAEEAVKDK-SRTEALEALGLDDEDARTATSLAAKTLCVPHDQPPL 556          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A1Z5KNG0_FISSO (Proline--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KNG0_FISSO)

HSP 1 Score: 96.7 bits (239), Expect = 1.450e-18
Identity = 60/102 (58.82%), Postives = 69/102 (67.65%), Query Frame = 0
Query:  207 LPALLPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFV--DAGDWED---KVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQPPL 303
            L A L A L+EIQ AMF KAK ARDA +V+VTEWKDFVP L + NLVLTP+   +  DWED   K SR    + +G   E    ATSVAAKTLCIPF+QP L
Sbjct:  816 LGARLKAKLDEIQGAMFVKAKEARDAHVVKVTEWKDFVPNLEKHNLVLTPWCGGEHQDWEDWVKKTSREESLKTLGQEGEDEKTATSVAAKTLCIPFNQPEL 917          
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Match: A0A7R9Z9S7_9STRA (Proline--tRNA ligase n=2 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9Z9S7_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 1.980e-18
Identity = 58/98 (59.18%), Postives = 64/98 (65.31%), Query Frame = 0
Query:  211 LPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRGNLVLTPFV--DAGDWEDKVSRLFPPEGV---GGGRETPAAATSVAAKTLCIPFDQPPL 303
            +P  LE IQ  MFAKAKAARD+ +VQVTEWKDFVP L   NLVLTP+   +  DWED V      E +   G   E    ATSVAAKTLCIPFDQP L
Sbjct:  861 IPEKLESIQKDMFAKAKAARDSHVVQVTEWKDFVPNLELNNLVLTPWCGGEHQDWEDWVKDKSREESLAARGEEEEDTRTATSVAAKTLCIPFDQPEL 958          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig9.85.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3XXY9_HETAK1.430e-3372.73Proline--tRNA ligase n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S2UX41_9STRA4.850e-2661.70Proline--tRNA ligase n=1 Tax=Fibrocapsa japonica T... [more]
A0A482UP51_9ARCH4.700e-2356.99Proline--tRNA ligase n=1 Tax=archaeon TaxID=190666... [more]
A0A7S0T0X0_9STRA3.820e-2258.89Proline--tRNA ligase n=1 Tax=Chromulina nebulosa T... [more]
W7TR23_9STRA1.460e-2058.89Proline--tRNA ligase n=2 Tax=Monodopsidaceae TaxID... [more]
A0A835YT63_9STRA7.110e-2055.56Proline--tRNA ligase n=1 Tax=Tribonema minus TaxID... [more]
A0A7S3H5B0_9STRA8.660e-2056.04Proline--tRNA ligase n=1 Tax=Spumella elongata Tax... [more]
F0YK75_AURAN4.620e-1956.31Proline--tRNA ligase n=1 Tax=Aureococcus anophagef... [more]
A0A1Z5KNG0_FISSO1.450e-1858.82Proline--tRNA ligase n=2 Tax=Fistulifera solaris T... [more]
A0A7R9Z9S7_9STRA1.980e-1859.18Proline--tRNA ligase n=2 Tax=Pseudictyota dubia Ta... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 234..304
e-value: 1.3E-12
score: 49.6
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 235..302
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 240..300
e-value: 6.0E-8
score: 32.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..159
NoneNo IPR availablePANTHERPTHR43382:SF2BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASEcoord: 211..302
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 211..302

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig9contigH-akashiwo_Contig9:5907673..5909123 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastp: OGS1.0 vs UniRef902022-09-16
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig9.85.1mRNA_H-akashiwo_Contig9.85.1Heterosigma akashiwo CCMP452mRNAH-akashiwo_Contig9 5904574..5909123 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_H-akashiwo_Contig9.85.1 ID=prot_H-akashiwo_Contig9.85.1|Name=mRNA_H-akashiwo_Contig9.85.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=305bp
MVMQALELYLAMYEELLACPWCGEEDRGARSSRRPDHHHSGGLHPRVRPG
HPGRHQPPPGPELRQDVRHPVRGRPGQEGPGLADLLGADHAHHRRDGHGA
RRRHGAGAAPPRGPRAGGGHPHPQQQVQAGGAAALLREGGGRPGGRGRAG
THRRPAELPRGLEVQPLGAEGRARARGGGAPGHGAGHRAAVPAPRQEQAG
TPRPPELPALLPALLEEIQAAMFAKAKAARDASLVQVTEWKDFVPALNRG
NLVLTPFVDAGDWEDKVSRLFPPEGVGGGRETPAAATSVAAKTLCIPFDQ
PPLA*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR017449Pro-tRNA_synth_II
IPR016061Pro-tRNA_ligase_II_C
IPR004499Pro-tRNA-ligase_IIa_arc-type