prot_H-akashiwo_Contig1189.5.1 (polypeptide) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A3R7XQ30_9PROT (Phosphoglycerate kinase n=3 Tax=cellular organisms TaxID=131567 RepID=A0A3R7XQ30_9PROT)

HSP 1 Score: 657 bits (1694), Expect = 3.090e-235
Identity = 336/400 (84.00%), Postives = 362/400 (90.50%), Query Frame = 0

Query:   40 MAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            MAKKSVGDL EA+LKGKKVLVRCDLNVPLDGKTITDDTRIRAS+PTVKYLMEKGAKVLLSSHLGRPK+GPEDKFSL PV+ RL+ELLGT   MAPDC+GD V    D ++EG V+LLEN RFYKEE KN  +FA+KLA++AD++VNDAFGTAHRAH STEGVTK LSPSVAGFLLQKELDYLDGAVS+P RPFAAIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL TGSSLVEEDQL+LAK+LE  AKEKGI+ ILP+DVIVAD F+ DA TQ VSVE IPDGWMGLDNGPESTKMIQ  L +CKT+IWNGPMGVFEFE FAKGTFDIADTLAELTG G TTIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAAL EA
Sbjct:    1 MAKKSVGDLTEADLKGKKVLVRCDLNVPLDGKTITDDTRIRASVPTVKYLMEKGAKVLLSSHLGRPKEGPEDKFSLGPVAPRLSELLGTTVEMAPDCIGDAVTAKVDAMSEGSVLLLENTRFYKEEEKNVPEFAEKLAANADMFVNDAFGTAHRAHGSTEGVTKYLSPSVAGFLLQKELDYLDGAVSDPKRPFAAIVGGSKVSSKIGVIESLMEKVDKIVIGGGMVFTFLKARGLETGSSLVEEDQLELAKKLEVIAKEKGIDFILPSDVIVADKFAADAATQTVSVEAIPDGWMGLDNGPESTKMIQAKLGECKTVIWNGPMGVFEFEAFAKGTFDIADTLAELTGSGCTTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALQEA 400          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A7S0Q943_9EUKA (Phosphoglycerate kinase n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0Q943_9EUKA)

HSP 1 Score: 648 bits (1672), Expect = 4.840e-231
Identity = 339/432 (78.47%), Postives = 373/432 (86.34%), Query Frame = 0

Query:    8 LMLALLFAAAAAFQKSFNGVARTVYTRTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            L+LA     +A    S + +  +  +R+  +EMAKKSVGDL E +LKGKKVLVRCDLNVPLDGKTITDDTRIRAS+PTVKYLMEKGAKVLL+SHLGRPKDGPEDKFSL PV+ RLTELLGTE  MAPDC+G+ V    D + EG V+LLEN RFYKEE KN  +FA+KLA+SAD++VNDAFGTAHRAH STEGVTK L P+VAGFLLQKELDYLDGAVS+P RPFAAIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL TGSSLVEEDQL+LA +LE  A EKGI+ ILPTDVIVAD F+ DA TQ VSVE IP+GWMGLDNGP+STKMIQ  L +CKT+IWNGPMGVFEFE F+KGTFDIADTLAELTG G TTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAAL EA
Sbjct:   23 LLLAAALGVSAYAPLSGSRITASRVSRSASIEMAKKSVGDLSEDDLKGKKVLVRCDLNVPLDGKTITDDTRIRASVPTVKYLMEKGAKVLLASHLGRPKDGPEDKFSLGPVAPRLTELLGTEVTMAPDCIGEGVAACVDAMEEGSVVLLENTRFYKEEEKNVAEFAEKLAASADIFVNDAFGTAHRAHGSTEGVTKFL-PAVAGFLLQKELDYLDGAVSDPKRPFAAIVGGSKVSSKIGVIESLMEKVDKIVIGGGMVFTFLKARGLETGSSLVEEDQLELAAKLEKIAAEKGIDFILPTDVIVADKFAADAATQTVSVEAIPEGWMGLDNGPDSTKMIQAKLGECKTVIWNGPMGVFEFEAFSKGTFDIADTLAELTGTGCTTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALQEA 453          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A7S2ZNW2_9RHOD (Phosphoglycerate kinase n=2 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S2ZNW2_9RHOD)

HSP 1 Score: 640 bits (1651), Expect = 3.520e-228
Identity = 321/402 (79.85%), Postives = 362/402 (90.05%), Query Frame = 0

Query:   37 VLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDE 438
            V  MAKKSVGDL   +L GKKVLVRCDLNVPLDG TITDDTR+RAS+PT++YLM+ GAKV+LSSHLGRPK GPE+KFSL PV++RL+ELLG +  MAPDCVG+EV  +   + +G V LLENVRFYKEE KN+ +FA+KLA++AD++VNDAFGTAHRAH ST GVT+ L PSV+GFLLQKELDYL+G V+NP RPFAAIVGGSKVSSKIGVIESL+ K DK+IIGGGMVFTFLKARGL+ GSSLVEED+LDLAK LEA+A EKG+E ILPTDV+VADAFS DANT+VVSV++IPDGWMGLDNGPESTKMIQ+ LS+CKT+IWNGPMGVFEFE FAKGTFDIADTLAELTG G TTIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAALDE
Sbjct:   30 VTMMAKKSVGDLSGEDLAGKKVLVRCDLNVPLDGATITDDTRVRASVPTIEYLMKNGAKVILSSHLGRPKSGPEEKFSLKPVASRLSELLGVDVTMAPDCVGEEVTSIVSSMDDGSVTLLENVRFYKEEEKNDPEFAEKLAANADMFVNDAFGTAHRAHGSTAGVTEFLKPSVSGFLLQKELDYLEGTVANPGRPFAAIVGGSKVSSKIGVIESLLGKVDKLIIGGGMVFTFLKARGLNVGSSLVEEDKLDLAKELEAKAAEKGVEFILPTDVVVADAFSADANTKVVSVDDIPDGWMGLDNGPESTKMIQDKLSECKTVIWNGPMGVFEFEAFAKGTFDIADTLAELTGNGVTTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDE 431          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A7S0F4H3_9EUKA (Phosphoglycerate kinase n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0F4H3_9EUKA)

HSP 1 Score: 637 bits (1644), Expect = 4.730e-227
Identity = 335/435 (77.01%), Postives = 374/435 (85.98%), Query Frame = 0

Query:   11 ALLFAAAAAFQKSFNG-----VARTVYTRTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGK-GATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            A+ F+AA+A   SFNG     ++++   R+  +EMAKKSVGD+ EA+LKGK+VLVRCDLNVPLDGKTITDDTRIRASIPTV+YL + GAKVLL+SHLGRPKDGPEDKFSLAPV+ RL+ELLG E  +APDC+GD V    D +++G V+LLEN RFYKEE KN   FA+KLA++AD++VNDAFGTAHRAH STEGVTK L  SVAGFLLQKELDYLDGAVS P RPF AIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL TGSSLVEEDQL+LAK+LE  AKEKGI+ ILPTDVIVAD F+ DA T+ V VEEIP GWMGLDNGPESTKMIQ  L +CKT+IWNGPMGVFEFE FAKGTFDIADTLAE+T K G  TIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAAL EA
Sbjct:    5 AVSFSAASA---SFNGAMPSRMSQSKVARSGAVEMAKKSVGDMKEADLKGKRVLVRCDLNVPLDGKTITDDTRIRASIPTVQYLQKMGAKVLLASHLGRPKDGPEDKFSLAPVAPRLSELLGCEVKLAPDCIGDGVKACVDGMSDGDVILLENTRFYKEEEKNVPAFAEKLAANADMFVNDAFGTAHRAHGSTEGVTKFLDTSVAGFLLQKELDYLDGAVSAPKRPFCAIVGGSKVSSKIGVIESLMTKVDKIVIGGGMVFTFLKARGLETGSSLVEEDQLELAKKLEVLAKEKGIDFILPTDVIVADKFAADAQTKCVKVEEIPAGWMGLDNGPESTKMIQAKLEECKTVIWNGPMGVFEFEAFAKGTFDIADTLAEITSKNGCITIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALQEA 436          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A7S3AND0_9EUKA (Phosphoglycerate kinase n=4 Tax=Eukaryota TaxID=2759 RepID=A0A7S3AND0_9EUKA)

HSP 1 Score: 633 bits (1633), Expect = 3.460e-225
Identity = 325/411 (79.08%), Postives = 358/411 (87.10%), Query Frame = 0

Query:   29 RTVYTRTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            R + +R   +EMAKKSVGDL EA+LKGKKVLVRCDLNVPLDGKTITDDTRIRAS+PTV+YL+ KGAKVLLSSHLGRPK GPEDKFSL P + RL+ELLG E  MAPDC+GD V +  D +A+G V+LLEN RFYKEE KN  +FA+KLA++ADL+VNDAFGTAHRAH STEGVTK LSPSVAGFLLQKELDYLDGAVS P RPFAAIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL TGSSLVEEDQL+LA +LE  A EKGI  ILP+DV+VAD F+ DA TQ V  + IP+GWMGLDNGPE+T  I + LSDCKT+IWNGPMGVFEFE F+KGTF IADTLAELTG G TTIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAAL EA
Sbjct:   38 RVIQSRVAPVEMAKKSVGDLTEADLKGKKVLVRCDLNVPLDGKTITDDTRIRASVPTVEYLVSKGAKVLLSSHLGRPKSGPEDKFSLGPCAPRLSELLGKEVKMAPDCIGDGVKECVDAMADGDVLLLENTRFYKEEEKNVPEFAEKLAANADLFVNDAFGTAHRAHGSTEGVTKFLSPSVAGFLLQKELDYLDGAVSEPKRPFAAIVGGSKVSSKIGVIESLMEKVDKIVIGGGMVFTFLKARGLETGSSLVEEDQLELASKLEKIAAEKGITFILPSDVVVADKFAADAATQTVKADAIPEGWMGLDNGPEATAEIIKELSDCKTVIWNGPMGVFEFEAFSKGTFAIADTLAELTGTGCTTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALQEA 448          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A0M0JJT3_9EUKA (Phosphoglycerate kinase (Fragment) n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JJT3_9EUKA)

HSP 1 Score: 634 bits (1636), Expect = 8.220e-225
Identity = 325/425 (76.47%), Postives = 365/425 (85.88%), Query Frame = 0

Query:   15 AAAAAFQKSFNGVARTVYTRTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            A AA+F      V  +  +RT+V+EM KKSVG L EA+LKGK+VLVRCDLNVPLDGKTITDDTRIRAS+PTVKYL+EKGAKVLL+SHLGRPKDGPEDKFSL PV+ R+ ELLG    MAPDC+GD V K  D++  G V+LLEN RFYKEE KN  +FA+KLAS+ADL+VNDAFGTAHRAHASTEGVTK L  SVAGFLLQKELDYLDGAVS P RPFAAIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL TG+SLVE DQL+LA +LE  AKEKGI+ ILP+DV++AD F+ DA TQ+V V  IP+GWMGLDNGP +TK I E LSDCKT+IWNGPMGVFEF+ F+KGT+D+A+ LA+LTG G TTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAAL EA
Sbjct:   77 AGAASFTLPTAHVGSSQVSRTSVVEMKKKSVGALTEADLKGKRVLVRCDLNVPLDGKTITDDTRIRASVPTVKYLVEKGAKVLLTSHLGRPKDGPEDKFSLGPVAPRMAELLGKPVRMAPDCIGDAVKKCVDEMQNGDVLLLENSRFYKEEEKNKPEFAEKLASNADLFVNDAFGTAHRAHASTEGVTKYLKTSVAGFLLQKELDYLDGAVSEPKRPFAAIVGGSKVSSKIGVIESLMTKVDKIVIGGGMVFTFLKARGLETGASLVEMDQLELATKLEKMAKEKGIDFILPSDVVIADKFAADAATQIVKVNAIPEGWMGLDNGPMATKEIIEKLSDCKTVIWNGPMGVFEFDAFSKGTYDVAEALAKLTGTGCTTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALQEA 501          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A8J5XF92_DIALT (Phosphoglycerate kinase n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XF92_DIALT)

HSP 1 Score: 630 bits (1626), Expect = 3.110e-224
Identity = 331/436 (75.92%), Postives = 377/436 (86.47%), Query Frame = 0

Query:    8 LMLALLFAAAAAFQ--KSFNGVARTVYT---RTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDE 438
            L+LAL+ A+AAA++   S    A  VY    R   ++M KKSVGDL +AELKGK+VLVRCDLNVPLDGK ITDDTRIRAS+PT+K+LM KGAKVLLSSHLGRPKDGPEDKFSLAP + R++ELLG E VMAPDC+GD V  +   + +G VMLLENVRFYKEE KN   FA+KLA++ADLYVNDAFGTAHRAH STEGVTK L PSVAGFLLQKELDYL+GAV +P RPFAAIVGGSKVSSKIGVIES++ K DK+IIGGGMVFTFLKARGLSTGSSLVEED++DLA+ LEA+AK KG++LILPTDV++AD F  +AN++ V V EIPDGWMGLD+G  STKMIQ+ LSDCKTIIWNGPMGVFEF+KFA GT  IA+TLA+LTGKGA TIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEG+VLPGVAAL+E
Sbjct:    5 LVLALVAASAAAYRMPSSRLSAASRVYASKARCADIQMVKKSVGDLTDAELKGKRVLVRCDLNVPLDGKKITDDTRIRASVPTIKHLMSKGAKVLLSSHLGRPKDGPEDKFSLAPCAVRMSELLGKEVVMAPDCIGDSVAALVAKMNDGDVMLLENVRFYKEEEKNVPAFAEKLAANADLYVNDAFGTAHRAHGSTEGVTKYLKPSVAGFLLQKELDYLEGAVQSPKRPFAAIVGGSKVSSKIGVIESMLDKVDKLIIGGGMVFTFLKARGLSTGSSLVEEDKVDLARSLEAKAKAKGVKLILPTDVVIADKFDANANSKTVPVTEIPDGWMGLDSGAASTKMIQDELSDCKTIIWNGPMGVFEFDKFAAGTTGIAETLAQLTGKGAITIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGQVLPGVAALNE 440          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A5J4ZAT6_PORPP (Phosphoglycerate kinase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4ZAT6_PORPP)

HSP 1 Score: 629 bits (1621), Expect = 2.000e-223
Identity = 315/397 (79.35%), Postives = 358/397 (90.18%), Query Frame = 0

Query:   42 KKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDE 438
            KKS+ DL +A+L+GK+V VRCDLNVPLDGKTITDDTR+RAS+PT+KYLME GAKV+LSSHLGRPK GPEDKFSLAPVS RL+ELLGT  VMAPDC+GD+V+ +  ++  G VMLLENVRFY EE KN+++FAKKLA++ADLYVNDAFGTAHRAH ST GVT+ +  SVAGFLL+KEL+YL GAVS P RPFAAIVGGSKVSSKIGVIESLM K DK++IGGGMVFTFLKARGL+ GSSLVEED+LDLAK LEA+AK KGI+ ILPTDV++AD F+ DANT+V SVE I DGWMGLDNGP+STKMIQ+ L+DCKT+IWNGPMGVFEFEKFAKGTFDIA+TLA LTGKG  TIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAAL++
Sbjct:   47 KKSIKDLSKADLEGKRVFVRCDLNVPLDGKTITDDTRVRASVPTIKYLMENGAKVILSSHLGRPKSGPEDKFSLAPVSTRLSELLGTNVVMAPDCIGDKVSALVGEMKNGDVMLLENVRFYPEEEKNDKEFAKKLAANADLYVNDAFGTAHRAHGSTAGVTEFVPVSVAGFLLEKELEYLAGAVSEPKRPFAAIVGGSKVSSKIGVIESLMEKVDKIVIGGGMVFTFLKARGLNVGSSLVEEDKLDLAKELEAKAKAKGIKFILPTDVVLADKFAADANTKVTSVEAIEDGWMGLDNGPDSTKMIQDELADCKTVIWNGPMGVFEFEKFAKGTFDIAETLAALTGKGTITIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALND 443          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A835YTR9_9STRA (Phosphoglycerate kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YTR9_9STRA)

HSP 1 Score: 629 bits (1621), Expect = 2.770e-223
Identity = 325/416 (78.12%), Postives = 362/416 (87.02%), Query Frame = 0

Query:   38 LEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLA--------------KRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            + M KKSVGDL +AELKGKKVLVRCDLNVPLDGKTITDDTR+RASIPTVKYL+EKGA+V LSSHLGRPK+GPEDKFSL+ V+ RLTELLG EC MAPDC G EV  M D L +G VMLLENVRFYKEE KN  +FA++LA+  D++VNDAFGTAHRAH STEGVTK LSPSVAGFLLQKELDYL+GAVS P RPFAAIVGGSKVSSKIGVIES++AK DK+IIGGGMVFTFLKARGL+TGSSLVEEDQL+LA              K+LE  AKEKG++LILP+DV++AD F+ DANTQVVS + IPDGWMGLDNGPE+TK IQ AL+DCKT+IWNGPMGVFE E FAKGTF +A+TLAE+T KG  TIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAAL+EA
Sbjct:   38 MTMVKKSVGDLSDAELKGKKVLVRCDLNVPLDGKTITDDTRVRASIPTVKYLLEKGARVALSSHLGRPKNGPEDKFSLSAVAPRLTELLGQECKMAPDCKGAEVKAMVDSLKDGEVMLLENVRFYKEEEKNVPEFAEELAAPFDMFVNDAFGTAHRAHGSTEGVTKFLSPSVAGFLLQKELDYLEGAVSEPKRPFAAIVGGSKVSSKIGVIESMLAKVDKLIIGGGMVFTFLKARGLNTGSSLVEEDQLELATKAIFAARDATCASKQLEKIAKEKGVQLILPSDVVIADKFAADANTQVVSSDAIPDGWMGLDNGPEATKEIQAALADCKTVIWNGPMGVFEMEAFAKGTFAVANTLAEITPKGCITIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALNEA 453          

BLAST of mRNA_H-akashiwo_Contig1189.5.1 vs. uniprot
Match: A0A0G4GAZ1_VITBC (Phosphoglycerate kinase n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4GAZ1_VITBC)

HSP 1 Score: 623 bits (1606), Expect = 3.820e-221
Identity = 318/418 (76.08%), Postives = 364/418 (87.08%), Query Frame = 0

Query:   22 KSFNGVARTVYTRTNVLEMAKKSVGDLGEAELKGKKVLVRCDLNVPLDGKTITDDTRIRASIPTVKYLMEKGAKVLLSSHLGRPKDGPEDKFSLAPVSARLTELLGTECVMAPDCVGDEVNKMADDLAEGGVMLLENVRFYKEETKNNEDFAKKLASSADLYVNDAFGTAHRAHASTEGVTKTLSPSVAGFLLQKELDYLDGAVSNPVRPFAAIVGGSKVSSKIGVIESLMAKCDKVIIGGGMVFTFLKARGLSTGSSLVEEDQLDLAKRLEAEAKEKGIELILPTDVIVADAFSPDANTQVVSVEEIPDGWMGLDNGPESTKMIQEALSDCKTIIWNGPMGVFEFEKFAKGTFDIADTLAELTGKGATTIIGGGDSVAAVEKAGLADKMSHISTGGGASLELLEGKVLPGVAALDEA 439
            +SF G++R        + MAKKSV  L E +L GK+VLVRCDLNVPLDGKTITDDTRIRASI T+KYL + GAKVLL+SHLGRPK+GPEDKFSL PV+ RL+ELLG +  +APDC+GD+V +M   +  G VMLLENVRFY EE KN+ DFAKKLA++ADL+VNDAFGTAHRAH STEGVTK L+PSVAGFLLQKELDYL+GAVS+P RPFAAIVGGSKVSSKI VIE+L+ K DK+IIGGGMVFTFLKARG+S G+SLVEED++DLAK++E  A  KG+ELILPTDVI+AD F+ DA ++VV V EIPDGWMGLDNGPESTKMIQ+ L++CKTI+WNGPMGVFEF+KFA GT D+A TLAELT KGATTIIGGGDSVAAVEKAGLA+KMSHISTGGGASLELLEGKVLPGVAALDEA
Sbjct:   32 RSFRGISRRA-----PMTMAKKSVSTLTETDLAGKRVLVRCDLNVPLDGKTITDDTRIRASIQTIKYLADNGAKVLLTSHLGRPKNGPEDKFSLGPVAGRLSELLGKDVPIAPDCIGDKVEEMVGKMNNGDVMLLENVRFYAEEEKNDPDFAKKLAANADLFVNDAFGTAHRAHGSTEGVTKFLAPSVAGFLLQKELDYLEGAVSDPKRPFAAIVGGSKVSSKITVIETLLEKVDKLIIGGGMVFTFLKARGMSVGASLVEEDKMDLAKKIEEIASSKGVELILPTDVIIADKFAADAESKVVPVGEIPDGWMGLDNGPESTKMIQDKLAECKTIVWNGPMGVFEFDKFAAGTIDVAKTLAELTEKGATTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKVLPGVAALDEA 444