prot_H-akashiwo_Contig1127.1.1 (polypeptide) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI000B8F2756 (uncharacterized protein LOC110913170 n=1 Tax=Helianthus annuus TaxID=4232 RepID=UPI000B8F2756)

HSP 1 Score: 195 bits (495), Expect = 2.160e-55
Identity = 120/321 (37.38%), Postives = 182/321 (56.70%), Query Frame = 0

Query:    4 KKRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPML--AHGRLTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVNE-TIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            ++RT++ ++AK L+V+ STV   IK+G ++     + PNLT ++K  RL F  S I P+ +      +Y M +  H+DEKWF++ + S+ Y L P E  P +K   KK  +K VMFL  VARP        +  GK+G +P  +L+  ++ SKN  AGT +     ++  E  +  +ID+VLPA+    P+       G    ++IQ D A  H  + +D +F+   + GGFD +LR QP  SPDLNVLDLGFF  +QSL   ++   S + L+ A   ++    +  LN+VFLTLQ+ M   ++V G NNY+ PH+ K R
Sbjct:   97 RRRTNIRSIAKALNVSKSTVHRRIKEGELRPHTNAIKPNLTEENKKERLEFCLSMITPSLSNPS---FYDMFNVVHIDEKWFYMSKPSKSYYLVPGEDEPLRKCKSKKFITK-VMFLAAVARPRFDTLGNEIFSGKIGIFPFTKLEPAKRSSKNRVAGTMETKPILSVTKEVTRSWLIDKVLPAIRDKWPQ-------GHTGPIFIQQDNAKPHISI-DDEQFVQEASRGGFDIRLRFQPPNSPDLNVLDLGFFRAIQSLQ-EQEVLGSIDELVHAVQTSFHKMSSRELNNVFLTLQTCMKEIMKVHGSNNYQTPHISKGR 404          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI001C25E3EC (uncharacterized protein LOC121760632 n=15 Tax=Salvia splendens TaxID=180675 RepID=UPI001C25E3EC)

HSP 1 Score: 189 bits (480), Expect = 8.930e-53
Identity = 112/320 (35.00%), Postives = 183/320 (57.19%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPMLAHGR--LTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+++  VA  ++V+ +T+  ++K+  +K   + + P LT  +K+ R+++  S+I PT    GK  Y+SM +  H+DEKWF++ +TS  Y L P+E  P +    K+  +K VMF+  V+RP+       + DGK+G +P  +    Q+ SKN   GT +    ++I  E     +I++++P++ A  P + S       K ++IQ D A  H + A D +F A  +  GF+F L  QP  SPD NVLDLG+F  +QSL    K   S + L++  + ++ +   + LN VF+TLQS +TA ++V G N+YK PHM K R
Sbjct:  118 ERSTIRKVASKMEVSKTTIGRFLKRNQLKPHTSAIKPTLTETNKIARIKWCLSHIQPTLA-EGKLLYHSMHNIVHIDEKWFYMTKTSDRYYLLPDEDVPYRSCKSKRFITK-VMFMAAVSRPLCGPDGDIIFDGKIGLFPFTEQIPAQRSSKNRPKGTMETKPIQSITKEVMTACLINQIIPSIKAKWPANAS-------KKIFIQQDNAKPHLRAA-DHQFEALASTDGFEFHLISQPPNSPDTNVLDLGYFRAIQSLQ-DDKMATSVDDLLRNVFNSFEELSPQTLNRVFITLQSCLTAILQVHGKNDYKIPHMNKNR 426          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI0009019CDE (uncharacterized protein LOC109159770 n=3 Tax=Ipomoea TaxID=4119 RepID=UPI0009019CDE)

HSP 1 Score: 185 bits (469), Expect = 1.130e-52
Identity = 119/298 (39.93%), Postives = 168/298 (56.38%), Query Frame = 0

Query:   27 IKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPM--LAHGRLTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +K+G +K     + P LT +++  RL+F  S I+ ++      F   M    H+DEKWFF+ RTSQ Y L PEE  P +    KK  +K VMFLC VARP   L    + DGK+G +P    +  ++ SKN  AGT +    E+++ E  ++ +ID VLPA+ A  P S S       K +YIQ D A  H  +  D EFL A    GFD  L  QP  SPD+NVLDLGFF  LQSL   ++  ++ + L+ A ++A+ +   E+L++VFLTLQ  M   ++  G NNYK PH+ K +
Sbjct:   10 VKEGSLKSHSNAIKPMLTEENRKVRLQFCISMIDSSSPHNNPCFI-DMYDRVHIDEKWFFLSRTSQKYYLLPEEDEPYRTCKSKKFITK-VMFLCAVARPRFDLERNEMFDGKIGMFPFVYKEPAKRRSKNREAGTLETKPIESVNKEVTRKWLIDYVLPAIRAKWPPSSS-------KTIYIQQDNARPHISM-NDVEFLEAAKKDGFDIHLTCQPPNSPDMNVLDLGFFRALQSLQ-HQEAPRNVDELVAATHKAFNEISVESLSNVFLTLQLCMVEVMKKFGGNNYKLPHINKQK 296          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI0020096116 (uncharacterized protein LOC125197723 n=1 Tax=Salvia hispanica TaxID=49212 RepID=UPI0020096116)

HSP 1 Score: 186 bits (473), Expect = 1.250e-52
Identity = 114/320 (35.62%), Postives = 180/320 (56.25%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPMLAHGRLT--DGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+    VA  ++V+ +TV  + K G+I+     + P LT  +K++R+R+  S+I P A   GK  Y++M +T H+DEKWF++ + S  Y L P+E  P +    K+  +K VMF+C V+RP       T  DGK+G +P  +    ++ SKN   GT +     +++ E  +  ++++++PA+ A  P + S       K +YIQ D A  H   + D +F A  ++ GF F L  QP+ SPD NVLDLGFF  +QSL    K   + + L+   + ++ +   + LN+VFLTLQS  +  +EV G NNYK PH+ K R
Sbjct:   29 ERSYYRKVASKMEVSKTTVGRWAKNGLIRPHTNAIKPALTEANKISRMRWCLSHIQP-AIYEGKLLYHAMLNTVHIDEKWFYMTKASDRYYLLPDEDEPYRSCKSKRFITK-VMFMCAVSRPQFGTDGQTIFDGKIGIFPFTEQVPAKRKSKNRPRGTLETKPIPSVNKEAMRECLLNQIIPAIKAKWPANAS-------KEIYIQQDNAKPHLN-SSDSQFEALASSDGFKFHLISQPANSPDTNVLDLGFFRAIQSLQ-DDKVATNIDQLLGNVWSSFEELTPQTLNNVFLTLQSCFSKILEVHGGNNYKIPHLNKER 337          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI001C264D25 (uncharacterized protein LOC121774216 n=2 Tax=Salvia splendens TaxID=180675 RepID=UPI001C264D25)

HSP 1 Score: 187 bits (474), Expect = 1.880e-52
Identity = 119/321 (37.07%), Postives = 179/321 (55.76%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPML-AHGRLT-DGKVGCWPVGQLKQQQKVSKNAAAGTW--KFVNETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+S+  +A  L+V+ ST+  ++K+G ++     + P LT  +K+ R R+  S + P  T GG  F   M +  H+DEKWF++ + S  Y L P+E  P +    K++ +K VMF+C V+RP   A+G+ T DGKVG +P  ++   Q+ SKN A GT   K +N ++     +  +I +++PA+ A  P        G  K +YIQ D A  H   A DPEF A   + GFD QL  QP  SPD N+LDLGFF  +QSL    K  K+ + L+     ++ +   + LN VFL+LQ+ +T  +   G N YK PH+ K R
Sbjct:   60 ERSSLRKMAVKLNVSKSTLGQWVKQGKLRPHTNAIKPALTNMNKIARARWSLSQLQPQITQGGVQFQ-GMHNVVHIDEKWFYMTKVSDRYYLLPDEDEPYRSCKSKRYITK-VMFMCAVSRPQFDANGQATYDGKVGIFPFTEVLPAQRKSKNRARGTMETKAIN-SVTKAVMRDCLIHKIIPAIKAKWPV-------GASKDIYIQQDNATPH-ITAMDPEFQAVAKSDGFDIQLICQPPNSPDTNILDLGFFRAIQSLQ-HEKPCKTVDELVGNVCSSFTELSPQTLNKVFLSLQACLTEILHCRGGNGYKVPHINKDR 368          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI001C27E83B (uncharacterized protein LOC121790904 n=2 Tax=Salvia splendens TaxID=180675 RepID=UPI001C27E83B)

HSP 1 Score: 187 bits (476), Expect = 2.300e-52
Identity = 111/320 (34.69%), Postives = 181/320 (56.56%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPMLAHGR--LTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+++  +A  LDV+ STV  ++K+  ++     + P L+  +K+ R+R+  S+I P   + G   Y+ M +  H+DEKWF++ +TS  Y L P+E  P +    K+  +K VMF+C V RP  A     + DGK+G +P   ++  ++ SKN   GT +    ++++ E  +  +I +++PA+ A  P ++S       K ++IQ D A  H     D +FL+  +  GF F +R QP QSPD NVLDLG+F  +QSL    K  K  + L++  + ++ +     LN+VFLTLQ  +T  ++V G N+YK PHM K R
Sbjct:   99 ERSNIRKLASKLDVSKSTVGRFVKQNQLRPHTNAIKPLLSASNKVARMRWGLSHIQPLI-VNGMLKYHPMHNVVHIDEKWFYMTKTSDRYYLLPDENEPYRSCKSKRFITK-VMFMCAVCRPHFAENGEVIFDGKIGIFPFTTMEPAKRRSKNRPRGTLETKPIQSVNKEVMRECLIQKIIPAIKAKWPENIS-------KEIFIQQDNAKPHIHH-NDADFLSVASTDGFQFHIRCQPPQSPDCNVLDLGYFRAIQSLQ-DDKVAKGVDDLLRNVHSSFDELSPYTLNNVFLTLQGCLTEILKVQGGNDYKTPHMNKER 407          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: A0A484N929_9ASTE (Uncharacterized protein n=1 Tax=Cuscuta campestris TaxID=132261 RepID=A0A484N929_9ASTE)

HSP 1 Score: 185 bits (470), Expect = 2.720e-52
Identity = 124/324 (38.27%), Postives = 176/324 (54.32%), Query Frame = 0

Query:    4 KKRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPMLAHGR--LTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVNET-IDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPE---GLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +KRT++ A+A  +    STV  ++K+GM++     + P LT  +K++RLRF    I P  TI     + S  +  H+DEKWFF+ +TSQ + L PEE+ P +    K+  +K VMFLCVV RP+    +  L DGKVG +   +  Q ++ SKN   G  +    T +  +  K M+I+ V+PA+    P  LS       K++ IQ D A  H +   D +F+AA  + GF   L  QP  SPDLNVLDLGFF  +QSL    K   +P     L+ A   A+     E LN V+L+ Q ++T  +E  G NNYK PHM K R
Sbjct:   12 RKRTNLRALAAAIGKPKSTVHEWVKRGMLRSHSNAIKPYLTEANKISRLRFCLDQIEPH-TIPNSPAFKSFHNVLHIDEKWFFMSKTSQKFYLLPEEEDPYRACKSKRFIAK-VMFLCVVGRPLFGENKELLRDGKVGIFHFTEKMQAKRKSKNRERGVVEVKAITSVTKQITKEMLINTVIPAIQEKWPSQLS-------KIIQIQQDNARPHIQ-GIDTDFVAAGNSNGFQITLCNQPPNSPDLNVLDLGFFRAIQSL----KDQCAPSTVADLLDAVEGAYNALSPETLNKVWLSYQQVLTKIMEHEGGNNYKLPHMGKDR 321          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI0020091A08 (uncharacterized protein LOC125206097 n=1 Tax=Salvia hispanica TaxID=49212 RepID=UPI0020091A08)

HSP 1 Score: 187 bits (474), Expect = 4.420e-52
Identity = 116/320 (36.25%), Postives = 182/320 (56.88%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPML-AHGRLT-DGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+S+  +A  L V+ +T+  +IK+  ++     + P LT  +KL RL++  + + P+ T  GK  ++ M +  H+DEKWF++ +TS  Y L P+E  P +    K++ +K VMF+C V+RP   + G+ T DGKVG +P  +    Q+ SKN   GT +     T++ E  +  +I +++PA+ A  P   S       K +YIQ D A  H   A DPEF A   + GF   L  QP  SPD N+LDLGFF  +QSL   +K  ++ + L++   +A+ +   + LN VFL+LQ+ +T  ++  GDN+YK PHM K R
Sbjct:   98 QRSSIRTMACNLSVSKTTIHNWIKQDKLRPHTNAIKPALTELNKLARLKWCLTQLQPSIT-QGKVHFHGMHNIVHIDEKWFYMTKTSDRYYLLPDEDEPYRSCKSKRYITK-VMFMCAVSRPQFDSEGQPTYDGKVGIFPFTEAVPAQRKSKNRDRGTLETKPITTLNKEVMRGCIIHKIIPAIKAKWPDWAS-------KEIYIQQDNATPH-ITATDPEFQAIANSDGFKIHLICQPPNSPDTNILDLGFFRAIQSLQ-HQKPCRNVDELVENVCKAYEELTPQTLNRVFLSLQACLTQILQFRGDNSYKVPHMNKDR 406          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: UPI001C2784BB (uncharacterized protein LOC121808713 n=8 Tax=Salvia splendens TaxID=180675 RepID=UPI001C2784BB)

HSP 1 Score: 186 bits (473), Expect = 1.340e-51
Identity = 116/320 (36.25%), Postives = 181/320 (56.56%), Query Frame = 0

Query:    5 KRTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPML-AHGR-LTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVN-ETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYKAR 321
            +R+S+  +A  +DV+ ST   ++K   I+     + P LT  +K+ R+R+  S+I PT +  GK  Y++M +  H+DEKWF++ +TS  Y L PEE  P +    K+  +K VMF+C V+RPM  + G+ + D K+G +P  Q     + SKN   GT +     +++ E  +  ++++++P + A  P + +       K +YIQ D A  H K + D +F A  +  GF F L  QP+ SPD NVLDLGFF  +QSL    K   + + L+   + ++ +   + LN VFLTLQS +T  +EV G NNYK PH+ K R
Sbjct:  136 ERSSIKKLAIKMDVSKSTAGRWVKGNKIRAHTNAIKPALTDVNKIARMRWSLSHIQPTIS-EGKLTYHAMHNIVHIDEKWFYMTKTSDRYYLLPEEDEPYRSCKSKRFITK-VMFMCAVSRPMFGSDGQPIFDSKIGIFPFTQQVPAMRKSKNRPRGTLETKPIPSVNKEAMRECLLNQIIPTIKAKWPANAN-------KEIYIQQDNAKPHLK-SSDLQFEAIASTDGFKFHLVSQPANSPDSNVLDLGFFRAIQSLQ-DDKLATNIDELLANVWSSFQELTPQTLNKVFLTLQSCLTKILEVHGGNNYKIPHLNKDR 444          

BLAST of mRNA_H-akashiwo_Contig1127.1.1 vs. uniprot
Match: A0A6A5AA16_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A5AA16_9STRA)

HSP 1 Score: 186 bits (471), Expect = 1.480e-51
Identity = 113/316 (35.76%), Postives = 173/316 (54.75%), Query Frame = 0

Query:    6 RTSVAAVAKYLDVAPSTVQLYIKKGMIKRSKAWVSPNLTTKHKLNRLRFIRSYINPTATIGGKYFYYSMRSTFHLDEKWFFICRTSQGYILTPEEQAPAQKVHHKKHTSKKVMFLCVVARPM--LAHGRLTDGKVGCWPVGQLKQQQKVSKNAAAGTWKFVNETIDYEGYKRMVIDRVLPALWAGCPRSLSHFANGQPKVLYIQDDGACAHGKLAEDPEFLAACTAGGFDFQLRRQPSQSPDLNVLDLGFFNHLQSLTWRRKFFKSPEGLIKAAYRAWLDSPNEALNDVFLTLQSIMTATIEVAGDNNYKFPHMYK 319
            R +   +AK   V  + +   +K   + R  + + P LT +H++ R  F +S+I       G+  ++ M    H+DEKWF+I + ++ + L  +E  P +K   K+H +K VMFLC VARP    AH ++ DGKVG WP  + +  ++ SKN   GT   V  T+    Y+R ++D+V+PA+ A  P         +   +Y+Q D A  H  + +DPE LAA    G++ QL  QP+QSPD NVLDLGFFN +QSL   +   ++ + LIK    A+ +     L+  F+TLQ +M   +  +GDN YK PH+ K
Sbjct:  100 RGNYRTMAKATGVPKTALWRLVKAKKLNRRTSRLRPMLTPQHRVARYNFAKSFIRDGYQ--GRRQWHDMLDCVHIDEKWFYITQVNRRFYLWHDEPTPQRKAQSKRHITK-VMFLCAVARPRHDSAHRKMWDGKVGLWPFVETQLAKRRSKNRDRGTPVTVPMTVTKPVYRRFLVDKVIPAIQAQWP-------GRRGATIYVQQDNAKPHVAV-DDPEVLAAGRKEGWNIQLMAQPAQSPDFNVLDLGFFNAIQSLQ-HQTTARTIDDLIKCVEDAFENLAWRVLDKTFMTLQKVMEEAMRSSGDNVYKLPHLKK 403