prot_H-akashiwo_Contig1022.4.1 (polypeptide) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: D7FUP7_ECTSI (DNA topoisomerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUP7_ECTSI)

HSP 1 Score: 525 bits (1351), Expect = 6.030e-174
Identity = 287/566 (50.71%), Postives = 359/566 (63.43%), Query Frame = 0

Query:  152 RFEQLGEGVISYGPCQFPTLGFIVERHKRIQAKNPPPPHT-------HAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGT-GNSTRVTRAN--FSKCGRCQQMMDLKPTGNGRRGGGGRLAYCRACTTGYPLPRNGRL--EPHPHTCPLCGFQ-------VLAVAQGKVRT-AGYAV-PHCFNIAPGGRCGASSATGGLRCFMCSANCALAGRIAGG 696
            RF++L EGVISYGPCQFPTLGFIV+R+KRIQA       +         P   GG A+A F+W R RL+DH  C +LYEM + E  VA VT +  R   K RP+P+ TVEL KRAS +  + SE +  VAE LY RGIISYPRTET+KF  + D+  ++G F  +P++G Y   LL+ GGF  PREG  DD AHPPIHP K + D N  ++ DEK++YELV KHFLACCSKDA+GH T+V    G E F A GLMV ERNWL++Y PFERW G++IP F  GQ F PS ++M EGRT+ PP LSE+DLI LMD  GIGTDATI+ HI+ IQ     R+YA KN Q RF P+ LG+AL DGY  +G  +  P LRA ME DC  I+ G   ++AVV  C++ MK  F+ C   A KLD+A+A HF  +G+ G  +R  R    FS+CG C   MDLK + +GR      L +     TG+PLP  G+L  +     CPLC FQ       VL+V+ G   T +GY V P+CFN AP    G   +    RCFMCSA+C LA R+ GG
Sbjct:    4 RFDELQEGVISYGPCQFPTLGFIVDRYKRIQAFQSEAFWSIKMEYAEDDPRSPGGKARADFSWQRGRLYDHACCAILYEMTV-EDGVAVVTSVEARRVTKARPIPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINTLLGDFRDNPRFGNYVGGLLDNGGFLWPREGRGDDSAHPPIHPTKSV-DPNSLQA-DEKKIYELVTKHFLACCSKDAVGHQTKVLANCGGEDFTATGLMVTERNWLDVYEPFERWTGRNIPEFFQGQEFVPSVLVMDEGRTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQ----QRKYAVKNEQDRFYPTTLGLALVDGYDNIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISKMKECFQTCQALAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRP-----LLFXXXXXTGFPLPHKGKLTKQDPEFECPLCHFQARFLHVLVLSVSTGNDYTGSGYTVCPYCFNNAPAAHGGEGQSQ--FRCFMCSASCPLAKRVQGG 555          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: B7G3B1_PHATC (DNA topoisomerase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7G3B1_PHATC)

HSP 1 Score: 532 bits (1370), Expect = 1.160e-173
Identity = 324/742 (43.67%), Postives = 427/742 (57.55%), Query Frame = 0

Query:    7 WNACQPIDLFHLPIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCT------QVFPSYNLNVIE---SSPISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGE-GVISYGPCQFPTLGFIVERHKRIQAKNPPP-------------PHTHAPAGDGGAAQAS--------FTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAG-HPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKH--IPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGTGNST-RVTRANFSKCGRCQQMMDLKPTGNGRRGGGGR------LAYCRACTTGYPLPRNGRLEPH--------PHTCPLCGFQVLAVAQGK-VRTAGYAV-PHCFNIAPGGRCGASSATGGLRCFMCS-ANCALAGRIAGG 696
            W+ C PI LF  PIE   R+DM+ LE+ LK  +R+AQ L+LWLDCDREGE I  EV  VC       QV+ +    V+       + ++   N+Y++              AV+AR  +DLR+G+AFTRFQT RLQ                  +F+   E GVISYGPCQFPTLGF+VER  RI+   P                H  +   D  AA+          F+W R  L+D L   VL+E CL E   A VT + GR   K RP+PL TVEL KRAS YLR+GSE     AEELY +G ISYPRTET++F P+ + R +I  F+    ++GAYA++LLN+ GFQ PR G  DDQAHPPI P K + D N  +   ++++Y L+ KH+LACCS+DA+G  T + V +G E F+A GLMVIE+NWLEIY+P+ERW      +P    G    P+S LMKEGR+ PP  +SE +LI+LMD +GIGTDATI+ HI TI     DR+YA K+ +Q+F+P+PLG+AL +GY  MG+ L  P LR +ME +C  +A+G K ++ ++   L  MK+ +E    +A KLDEA+A HF  +G G ST +V   +FS+CG C+  M LK           R      L YC  C  G+ LPR G + P         P  CP+C FQV+ + +G+     GY V P CF+  P    GAS+A G  RCF C    CALA    GG
Sbjct:  132 WSKCDPIALFEAPIETSYRDDMQPLERMLKSLSRQAQVLILWLDCDREGEAIGDEVRTVCLGSNSRLQVYRARFSTVLPVEIERALQTLGRVNEYMVA-------------AVQARSTLDLRVGAAFTRFQTLRLQR-----------------KFDGFAEQGVISYGPCQFPTLGFVVERWARIETFIPEDFWHLELAISVDDTYHQQSQEEDQSAARGRTQQNRTIHFSWKRGYLYDKLLTTVLFEECL-EAGEAVVTAMNGRTRNKWRPVPLATVELQKRASMYLRIGSETLMSAAEELYQQGYISYPRTETERFRPEFEHRPLIQQFSSLQGEFGAYASKLLNENGFQIPRAGKSDDQAHPPITPAKAV-DPNTIQDQIQRKIYSLIVKHYLACCSRDAVGRGTTLTVRMGTEEFNATGLMVIEKNWLEIYSPWERWGSGQGELPPLQVGSRIRPTSFLMKEGRSGPPQPISEVELISLMDRNGIGTDATIAQHISTIL----DREYARKDGRQKFLPTPLGIALVEGYNSMGYQLNKPDLRREMEAECNEVASGRKTKEEIMVPILAKMKSCYETARAEARKLDEAVARHFPRLGAGESTSQVVEESFSECGVCRNSMALKQERENNNRTTARNTVRRKLLYCSTCRAGWTLPR-GVVRPKTEQEDNGPPVKCPICQFQVIRILRGEGYEGNGYHVCPKCFSDPPSDHGGASNA-GDFRCFACQHPTCALASGTPGG 835          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A067D7B1_SAPPC (DNA topoisomerase n=3 Tax=Saprolegnia TaxID=4769 RepID=A0A067D7B1_SAPPC)

HSP 1 Score: 525 bits (1351), Expect = 6.460e-170
Identity = 309/699 (44.21%), Postives = 399/699 (57.08%), Query Frame = 0

Query:    5 RKWNACQPIDLFHLPIEKYVRED--MKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQVFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKED----AVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERHKRIQA-KNPPPPHTHAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAK-NAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAI----GTGNSTRVTRANFSKCGRCQQMMDLKPTGNGRRGGGGRLAYCRACTTGYPLPRNGRLEPHPHTCPLCGFQVLAVAQGKVRTAGYAV-PHCFNIAPGGRCGASSATGGLRCFMCSANCALA 690
            R W  C P+DLF   +EK VRED   K++EQ LK EA +AQ LVLWLDCDREGENIA+EV  VC +      +     S +             H +   + Q  +    A +AR EIDLRLG+AFTRFQT R+Q   P      E+              V+S+G CQFPTLGF+V+RH  I+A       H H            FTW R R++DH+A L LYE C+ E  +A +  I  +DT K++P PL TVE  KRAS +LR+ SE +   AE LY RG++SYPRTET  F   TDLR ++   AGH  WG Y   L N G F+ PR G  DDQAHPPIHP KC+   +   ++ E+R+YELV +HFLACCS DA G  T V + L +E F A+GLM++ RN+L+IY  +E+W    IP++  G  F P+++L+K G T PPP L+E+DLIA MD HGIGTDATI+ HI TI      RQYA K N   +F P+ LG+AL   Y  MG+ L  P LRA +ERDC AIA G K    VV  C+  M+T+++    ++  LDE    +F       G GNS RV    FS CG+C Q M+L+     ++   G L  CR C   Y LP    ++     CPLC FQV+ + Q    T  Y+V PHCFN  P  R         + C+ C+A+CALA
Sbjct:   70 RGWKTCDPVDLFSAAVEKTVREDSTQKKIEQTLKAEAARAQMLVLWLDCDREGENIAFEVKAVCEKANRRLRVKRARFSALIPRDI--------HAAVQNLVQPNENLSLACDARSEIDLRLGAAFTRFQTMRIQKKFPNVQANSESK-------------VVSFGSCQFPTLGFVVDRHLAIEAFVRELFYHIHVTHAIPDVGSIVFTWGRGRVYDHIAALALYESCV-EAQIATINSISKKDTSKRKPFPLTTVEFQKRASRWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLRALLDLHAGHETWGGYVAALQN-GKFEAPRSGKHDDQAHPPIHPTKCVPLSS-LNNDVERRVYELVVRHFLACCSMDARGSQTNVTMTLASETFTASGLMILARNYLDIYV-YEKWAATVIPVYERGDRFVPTTLLLKRGETAPPPLLTESDLIAKMDTHGIGTDATIAEHINTILK----RQYAIKVNNNTQFKPTELGLALVQTYDRMGYQLAKPDLRAAVERDCNAIAQGQKSCDQVVRSCMDQMRTIYQNVVARSGVLDETFQGYFGVANQDAGAGNS-RVVDGAFSACGKCHQPMELR-----KKPDDGSLLVCRRCDDQYALPMLANIQKSTTRCPLCQFQVVDI-QRSAGTNVYSVCPHCFNHPPNARLNE------MPCYSCTADCALA 726          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A1V9Z583_9STRA (DNA topoisomerase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Z583_9STRA)

HSP 1 Score: 523 bits (1347), Expect = 2.350e-169
Identity = 314/697 (45.05%), Postives = 406/697 (58.25%), Query Frame = 0

Query:    5 RKWNACQPIDLFHLPIEKYVRED--MKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQVFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERHKRIQA-KNPPPPHTHAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSND-EKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAK-NAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHF-----DAIGTGNSTRVTRANFSKCGRCQQMMDLKPTGNGRRGGGGRLAYCRACTTGYPLPRNGRLEPHPHTCPLCGFQVLAVAQGKVRTAGYAV-PHCFNIAPGGRCGASSATGGLRCFMCSANCALA 690
            R W  C PIDLF   +EK VRED   K++ Q L+ EA +AQ LVLWLDCDREGENIA+EV  VC +      +     S +              P+ +       A +AR EIDLRLG+AFTRFQT R+Q   P      E+              V+S+G CQFPTLGF+V+R   IQA    P  H H            F+W+R R+FD +A L L+E C+ +  VA VT++  ++T K++PLPL TVE  KRAS +LR+ SE +   AE LY RG++SYPRTET  F   TDL  ++     H  WG Y N LL  G F+ PR G  DDQAHPPIHP K +       +ND E+R+YE V +HFLACCS+DA G  T V + L  E F A GLM++ERN+L+IY  +E+W    IP++  G TF P+++L+K G T PPP L+E+DLIA MD HGIGTDATI+ HI TI      RQYA K N   +F P+ LG+AL   Y  MG+ L  P LRA MERDC AIA G K  + VV +C++ MKT+FE+   ++  LDE    +F     +A+   NS  V RA FS CG+CQQ M+L+     ++   G L  CR C   Y LP +  +      CPLC FQV+ + Q    +AGY+V PHCFN  PGGR         + C+ C+A+C LA
Sbjct:   70 RGWKTCDPIDLFTAAVEKTVREDSTQKKIAQTLRAEASRAQMLVLWLDCDREGENIAFEVKAVCEKANRRLRVLRARFSALIPRDIQRAVQSLVQPNENLSL----ACDARSEIDLRLGAAFTRFQTMRIQKKFPNVQVTSESR-------------VVSFGSCQFPTLGFVVDRFLAIQAFVREPFYHIHVAHTIPDVGSIVFSWNRARVFDFVAGLALFETCV-DAQVATVTDVTKKETSKRKPLPLTTVEFQKRASRWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLPALLALHTDHEMWGGYVN-LLQGGKFEAPRRGSHDDQAHPPIHPTKSVA--LAAITNDVERRVYEFVVRHFLACCSRDARGSQTSVAMRLADETFGAKGLMILERNYLDIYK-YEKWAATTIPVYERGDTFVPTTLLLKAGETAPPPLLTESDLIAKMDQHGIGTDATIAEHINTILK----RQYAVKVNNDTQFQPTELGLALVQTYDRMGYQLAKPDLRAAMERDCNAIAQGQKTCEQVVRDCMSQMKTIFESVVARSNVLDETFQGYFGEATPEALAV-NSRVVDRA-FSACGKCQQPMELR-----KKSDDGGLLVCRRCNELYALPLHSAVAQSATRCPLCHFQVVEI-QKAAGSAGYSVCPHCFNAPPGGRLNE------MACYQCTADCPLA 726          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A7S4EJM4_9STRA (DNA topoisomerase n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4EJM4_9STRA)

HSP 1 Score: 519 bits (1336), Expect = 2.950e-168
Identity = 322/778 (41.39%), Postives = 418/778 (53.73%), Query Frame = 0

Query:    7 WNACQPIDLFHLPIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQ--------VFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGE-----GVISYGPCQFPTLGFIVERHKRI-------------------QAKNPPPPHTHAPAGDGG------------------AAQASFTWDRNRLFDHLACLVLYEMCL-AEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQ----WGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKH--IPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGTGNS-TRVTRANFSKCGRCQQMMDLKPTGNGRRGGGG---------------RLAYCRACTTGYPLPRNGRLEPH----------PHTCPLCGFQVLAVAQGK-VRTAGYAV-PHCFNIAPG--GRCGASSATGGLRCFMCS-ANCALAGRIAGG 696
            W+   P+ LF  P+E   R+DM  L Q L   A+    LVLWLDCDREGE IA EV+ VC +        V+ +    V+    I ++    +       ++H +     AV+AR E+DLR+G+AFTRFQT RLQ               ++ +    GE     GV+SYGPCQFPTLGF+VER  RI                   QA  PPPP   AP  DG                   A   +FTW R RL+D LA L +YE CL   PCVA VT++ GR   K RP+PL T+EL KRAS YLR+GSE     AEELY +G+ISYPRTET++F P+ D   ++  F    +    + +YAN LLN+ GFQ PR G  DDQAHPPI P K + D N  + + ++ +Y LV KH+LACCS+DA+G  T + V + +E F A GLM+ ++NWLEIY P+ERW+     +P    G   AP S++MKEG+T PP  +SE +LI LMD  GIGTDATI+ HI TIQ    DR Y  K+   RF+P+PLG+AL +GY  MG+ L  P LR + E +C  +A   K ++ +V   L  MK  +   T+ A KLD+AMA HF  IG GN  T V +A FS CG CQQ M LK                          +L +C AC  GY +PR G+L P           P  CP+C FQV+++ +G      GY + P C++  P   G            CF CS   CALAG   GG
Sbjct:   91 WSRVDPVQLFEAPLEIIYRDDMVPLRQMLTNLAKSVDGLVLWLDCDREGEAIADEVLRVCLESNRRLEPYVYRAKFSTVLAPEIIRALGTLGRI------NNHFVQ----AVQARSELDLRVGAAFTRFQTLRLQ---------------KKFQLPGAGENGGSSGVVSYGPCQFPTLGFVVERWARIEIFVPDDFWFLELTLKVPQQAVAPPPPA--APDTDGSNRTNNADRXXXXXXXXXNARPIAFTWKRQRLYDRLATLAIYESCLDTTPCVATVTKLEGRPKNKWRPVPLATIELQKRASRYLRIGSETLMSAAEELYQQGLISYPRTETERFRPEFDHHALLRDFCSLDEAAGPYASYANHLLNENGFQNPRAGQNDDQAHPPITPCKVV-DPNTIQDHTQRNVYILVSKHYLACCSRDAVGRETSLTVRIASEEFTAKGLMIEQKNWLEIYHPWERWSTGQGELPHLMVGSRIAPHSLVMKEGQTTPPLPISEPELITLMDKAGIGTDATIAQHITTIQ----DRDYVTKDGNLRFLPTPLGIALVEGYNSMGYQLNKPDLRRETEHECNLVANRQKTKEEIVPLILNKMKDCYLKATRDARKLDQAMARHFQRIGAGNDHTEVVQAQFSTCGNCQQPMALKQERRXXXXXXXXXXXXXXXXXXDVPRKLLFCNACQEGYVMPRRGQLRPKTSEEGNNDNTPVKCPICNFQVISIVRGDGYEGNGYNICPKCYSDPPADHGXXXXXXXXTNHPCFSCSHPTCALAGGTQGG 836          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A6A5C7F2_NAEFO (DNA topoisomerase n=1 Tax=Naegleria fowleri TaxID=5763 RepID=A0A6A5C7F2_NAEFO)

HSP 1 Score: 508 bits (1309), Expect = 4.100e-165
Identity = 304/693 (43.87%), Postives = 405/693 (58.44%), Query Frame = 0

Query:    5 RKWNACQPIDLFHL--PIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQVFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKED----AVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERH---KRIQAKNPPPPHTHAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNP--DTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGTGNSTRVTRANFSKCGRCQQMMDLKPTGNGRRGGGGRLAYCRACTTGYPLPRNGRLEPHPHTCPLCGFQVLAVAQGKVRTAGYAV-PHCFNIAPG-GRCGASSATGGLRCFMCS 684
            R W +C PI L  +  P+ + V E   ++E+ L+ EAR A  L+LWLDCDREGENIA+EVI VC +V  + +   IE++ I    F        + +   + +       AV+ARQE+DLR+G++FTRFQ+ RLQ                  +F  L   VISYGPCQFPTLGF+V+R    K  +++     +     G+ G AQ  F W R RLFDHL CLVLYE+C+  P  A VT +  +   KQRP+PL T+EL K AS  LRM S  + KVAE LY +G ISYPRTET  +N   D DL+ +I   + +P WG YA+ LLN GG+++P  G +DD+AHPPIHP+K     ND K  D   LYEL+ +HFLACCS+DALG  T+V + +  E F  +GLMV ++N+LEI+  +++W+ K IP+F   Q F P+S+LM EG T PPP+LSE+DLI+ MD +GIGTDAT++ HIKTIQ    DR Y  K    RF  + LG+AL  GY  +G+ L  P LRA+ME+D   I+ G   + AV+ + L   K VF    + A  LD+A+ +HF  IG    ++V   +FS CG C   MDLK         G R  +C+ C   Y LP     E     CP+C FQVL V   K     Y + P+CFN  P     GA +   G RCF C+
Sbjct:   74 RSWASCDPIVLMDVSTPVIRTVPEKNAEIEKNLENEARNASKLILWLDCDREGENIAFEVIEVCKRVNRTLS---IENNTILRARFSEFTPAAINRAIRNLERPNVLDSMAVDARQELDLRIGASFTRFQSMRLQK-----------------KFHGLPGDVISYGPCQFPTLGFVVKRDLLIKNFESEKFWTLNCSHDMGEEGKAQ--FLWKRGRLFDHLTCLVLYELCVENP-TATVTRVDKKRRTKQRPVPLNTIELQKLASQKLRMSSHDTMKVAESLYNKGFISYPRTETNNYNQFSDDDLKNLIQEHSTNPVWGQYASDLLN-GGYRKPTSGKQDDKAHPPIHPLKGSCQTNDQKEQD---LYELISRHFLACCSQDALGSETKVHIVIANEEFTCSGLMVEQKNYLEIWK-YDKWSDKSIPVFQQNQQFVPTSLLMDEGETRPPPHLSESDLISAMDSNGIGTDATVAQHIKTIQ----DRGYVIKRGN-RFEATNLGIALLQGYDLIGYELSTPNLRAKMEKDMMCISQGQITKDAVINQSLAMYKHVFMQICKSAHLLDKAVQQHFKPIGESGQSKVLVRSFSMCG-CGNSMDLKEQD------GHRFLFCKNCKEAYNLPSKCEYESISDQCPICNFQVLDVTTQK--NTNYKICPNCFNNPPDLENAGAETHANGFRCFSCT 724          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A1E7EWM7_9STRA (DNA topoisomerase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EWM7_9STRA)

HSP 1 Score: 506 bits (1303), Expect = 5.120e-165
Identity = 314/752 (41.76%), Postives = 414/752 (55.05%), Query Frame = 0

Query:    5 RKWNACQPIDLFHLPIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQ--------VFPSYNLNVIESS---PISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEG---VISYGPCQFPTLGFIVERHKRIQ------------AKNPPPPHTHAPAGDG---GAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQ----WGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKH--IPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGTGNSTRVTRANFSKCGRCQQMMDLKP-------TGNGRRGG-GGR-----------LAYCRACTTGYPLPRNGRLEPH--------PHTCPLCGFQVLAVAQGKVRTA-GYAV-PHCFNIAPGGRCGASSATGGLRCFMCS-ANCALAG 691
            ++W  C P++LF  P+E    EDM++L + L  E++K   LVLWLDCDREGE IA EV+ VC          V+ +    V++      ++++   N Y +Q             AV+AR E+DLR+G+AFTRFQT RLQ               ++      GEG   V+SYGPCQFPTLGF+VER  RI+            +    PP +    GD     A   +FTW R                  +P  A V  + GR   K RPLPL T+EL KRAS YLR+GSE   K AE+LY  G ISYPRTET++F P+     +I  FA        + +YAN+LLN+ G+Q PR GP DDQAHPPI P K + D N  +   ++++Y LV KH+LACCS+DA+G  T + +++ +E F A GLMV ++NWLEIY P+ERW+     +P    G    P S++MKEG+T PP  +SE +LI +MD  GIGTDATI+ HI TIQ     R YA K+  QRF P+ LG+AL +GY  MG+ L  P LR + ER+C  +A G K +  +V   L  MK  +   T+ A KLD+AMA H+  +GTGN T V +A FS+CG CQ+ M LK        +G  RRGG GGR           L +C  C  G+ LPR G   P         P  C +C FQV+AVA+G   T  GY + P C+N +P      S+  G   CF CS   CALAG
Sbjct:  115 KQWGRCDPLELFEAPLEVTYSEDMERLRRMLANESKKVDVLVLWLDCDREGEAIADEVLQVCVDSNRRLIPNVYRAKFSTVMDGEIKRALNTLGRINDYFVQ-------------AVKARTELDLRVGAAFTRFQTTRLQ---------------KKFTLPGNGEGGNSVVSYGPCQFPTLGFVVERWARIETFVQDDFWFLELSLQVQPPSSGIDGGDNRNNNARPITFTWKR------------------QPNTATVISLEGRPKNKWRPLPLATIELQKRASRYLRIGSETLMKAAEQLYQNGFISYPRTETERFRPEFQHHPLIRDFASLSDAAGPYASYANQLLNENGYQPPRAGPNDDQAHPPITPCKVV-DPNTIQDLTQQKVYILVVKHYLACCSRDAVGRETSLTLKIASEEFTAKGLMVEQKNWLEIYHPYERWSTGQGELPHLRVGSRVIPESLVMKEGKTTPPQPISEPELITIMDKCGIGTDATIAQHIATIQ----QRDYATKDNNQRFSPTKLGIALVEGYNNMGYQLNKPDLRRETERECNLVANGQKSKDDIVGPILDKMKDCYRVATRDAHKLDQAMARHYSRVGTGNDTEVLQAQFSECGNCQRSMALKQERRTGNNSGGARRGGQGGRGRGNSSSEPKKLLFCNTCQEGWMLPR-GECRPKIEEGANASPVKCAICDFQVIAVARGGGYTGNGYNICPKCYNDSPQEY---SNGGGNHACFGCSHPTCALAG 811          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A448Z093_9STRA (DNA topoisomerase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z093_9STRA)

HSP 1 Score: 509 bits (1311), Expect = 7.010e-165
Identity = 324/756 (42.86%), Postives = 410/756 (54.23%), Query Frame = 0

Query:    7 WNACQPIDLFHLPIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQ--------VFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERHKRIQAKNPPP----------PHTHAPAGDG-------GAAQAS----FTWDRNRLFDHLACLVLYEMCL-AEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTF-----AGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKH--IPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDAIGTGNS-TRVTRANFSKCGRCQQMMDLKPTGNGRRGGGG-----------RLAYCRACTTGYPLPRNGRLEPH------------PHTCPLCGFQVLAVAQGK-VRTAGYAV-PHCFNIAPG--GRCGASSATGGLRCFMCS-ANCALAGRIAGG 696
            W+ C PI LF  P+E   R+DM  L + L   A+    LVLWLDCDREGE IA EV+ VC +        V+ +    V+    + ++    +        +H   Q   AV+AR E+DLR+G+AFTRFQT RLQ         +   AG   R    G GV+SYGPCQFPTLGF+VER  RI+   P            P     A DG       GA   S    F W R RLFD  A L +YE CL  EPCVA VT + GR   K RP+PL T+EL KRAS YLR+GSE     AEELY +G+ISYPRTET++F P+ D R +I  F     A  P + +YAN LLN+ GFQ PR G  DDQAHPPI P K + D    + + ++ +Y LV KH+LACCS+DA+G  T + V + +E F A GLM+ ++NWLEIY P+ERW+     +P    G   AP S +MKEG+T PP  +SE +LI LMD  GIGTDATI+ HI TIQ    DR YA K+  QRF+P+PLG+AL +GY  MG+ L  P LR + E +C  +A   K ++ +V   L  MK V+    ++A KLD++MA  F  IG GN  T + +A FS+CG CQQ M LK                      +L +C AC  GY LPR G   P                     FQV+A+A+G      GY V P C+N  P   G            CF CS   CALAG   GG
Sbjct:  103 WSRCDPIQLFEAPLEIVYRDDMVPLRRMLTNLAKNVDGLVLWLDCDREGEAIADEVLRVCLESNRRLEPYVYRAKFSTVLGPEILRALGTLGRI-------NHHFVQ---AVQARSELDLRVGAAFTRFQTLRLQKKF------QLPGAGEDGR----GAGVVSYGPCQFPTLGFVVERWARIEIFVPEDFWFLELTLRVPRQAGAADDGRGSGGGRGAGNDSRPIAFAWKRQRLFDRFATLAIYESCLDTEPCVATVTRLEGRPKNKWRPIPLATIELQKRASRYLRIGSETLMSAAEELYQKGLISYPRTETERFRPEFDHRALIRDFCTLADAAGP-YASYANHLLNENGFQNPRAGQNDDQAHPPITPCKVV-DPATIQDHTQRNVYVLVAKHYLACCSRDAIGRETSLTVRIASEEFTAKGLMIEQKNWLEIYHPWERWSTGQGELPHVMVGSRIAPHSFVMKEGQTTPPLPISEPELITLMDKSGIGTDATIATHIATIQ----DRDYATKDGNQRFLPTPLGIALVEGYNSMGYQLNKPDLRRETEHECNLVANRQKTKEEIVPLILNKMKDVYLTAAREARKLDQSMARRFQRIGAGNDHTELVQARFSQCGNCQQPMSLKQERRTXXXXXXXXXXXXXDTPRKLLFCNACQEGYILPRKGIPRPKISEXXXXXXXXXXXXXXXXNFQVVAIARGDGYEGNGYNVCPKCYNDPPADHGXXXXXXXXXTHPCFSCSHPACALAGGTQGG 832          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A0F7UL88_NEOCL (DNA topoisomerase n=1 Tax=Neospora caninum (strain Liverpool) TaxID=572307 RepID=A0A0F7UL88_NEOCL)

HSP 1 Score: 502 bits (1293), Expect = 8.110e-164
Identity = 305/738 (41.33%), Postives = 393/738 (53.25%), Query Frame = 0

Query:    5 RKWNACQPIDLFHLPIEKYVREDMKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQVFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKEDAVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERHKRIQ---------------------AKNPPPPHTHAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSNDEKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAKNAQQRFIPSPLGVALSDGYTEM----GHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHF----DAIGTGNSTRVTRANFSKCGRCQQMMDLK-----------PTGNGRRGGGG---------------RLAYC----RACTTGYPLPRNGRLEPHPHTCPLCGFQVLAVAQGKVRTAGYAVPHCFNIAPGGRCGASSATGGLRCFMC 683
            + WN   P DL+  P+EK + +D   +++ L + A+KAQWLVLWLDCDREGENIA+EV++VC++      +     S ++             P  ++     DAV+ R EIDLR+G++FTRF T R                  R R + L    ISYGPCQ PTLGF+V R   ++                       N PP                FTWDR RLFD LA LVLYEMCL  P VA VT++RG +  K RP+PL TVEL K AS  LRM S     +AE LY RGIISYPRTET+ F+P  DL  +I    G   WGAYA +L+ +G F  PR+G +DD+AHPPIHP+K +   ++F+  DE +LYE + +HFLACCS+DA G  T+V +++  E FHA GL V+ER WLE Y P+E+W+G  +P F   +   P+S+LM E  TEPPP L+EADLI LMD +GIGTDAT+  HI+TIQ    DR Y  KN   +F+P+ LGVAL  G+  +    G  L  P LRA+ME D A +A G K +Q V+   L+ M+ VF      A  +D  M        +++G    T V  ANFS+CG+C+  MDLK           P G GRR G G               R   C    + C T   LP  G L PH   C  C FQ L+V     +      P CF   P            LRCF C
Sbjct:   74 QNWNTTPPEDLYSAPVEKSISQDAGDVQKNLVDLAKKAQWLVLWLDCDREGENIAFEVLSVCSKANSRLKVFRAVFSALTKADLERACRSLQAPDRNQ----SDAVDTRSEIDLRIGASFTRFLTMRY-----------------RTRVK-LPSPTISYGPCQSPTLGFVVSRFVEVENFVREAYWTIRVTINKLDDEKGPNAPP------------LSVDFTWDRQRLFDQLAVLVLYEMCLENP-VATVTDVRGTERTKPRPVPLCTVELHKMASRKLRMSSAQCMSIAESLYQRGIISYPRTETEVFSPTMDLLALIRVHTGSTAWGAYAQKLV-EGDFLWPRDGARDDKAHPPIHPLKLMQR-SEFEKEDEWKLYEFITRHFLACCSEDAFGFETRVEIDIAGEGFHATGLTVLERRWLEAY-PYEQWSGTRLPRFLVNERVMPTSMLMSERHTEPPPLLTEADLIDLMDKNGIGTDATMHDHIRTIQ----DRHYCYKNENMQFVPTDLGVALYQGFKRLAEVSGVDLSLPDLRARMEADMALVARGAKTKQEVLDIHLSEMRRVFLLLKAHAAHMDREMRFRLGGLEESVGRDGVTLV--ANFSECGKCRAPMDLKASGGPRGDGSEPGGRGRRRGRGGRHPTTAELTSAVANRFLVCTRLSQGCQTVLLLPPKGDLAPHAVRCAYCSFQALSVTNTHTKKTHQLCPQCFRNPPSEEASHLQGEATLRCFAC 767          

BLAST of mRNA_H-akashiwo_Contig1022.4.1 vs. uniprot
Match: A0A1V9Y6X7_9STRA (DNA topoisomerase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9Y6X7_9STRA)

HSP 1 Score: 505 bits (1300), Expect = 4.230e-163
Identity = 303/700 (43.29%), Postives = 400/700 (57.14%), Query Frame = 0

Query:    5 RKWNACQPIDLFHLPIEKYVRED--MKQLEQQLKEEARKAQWLVLWLDCDREGENIAYEVINVCTQVFPSYNLNVIESSPISSVSFYNKYLLQYHPSSHRISQKED----AVEARQEIDLRLGSAFTRFQTQRLQAAPPRAPPRREAAAGRRARFEQLGEGVISYGPCQFPTLGFIVERHKRIQA-KNPPPPHTHAPAGDGGAAQASFTWDRNRLFDHLACLVLYEMCLAEPCVARVTEIRGRDTVKQRPLPLETVELTKRASNYLRMGSEHSTKVAEELYMRGIISYPRTETQKFNPDTDLRGMIGTFAGHPQWGAYANRLLNQGGFQQPREGPKDDQAHPPIHPVKCITDDNDFKSND-EKRLYELVCKHFLACCSKDALGHSTQVFVELGAERFHANGLMVIERNWLEIYAPFERWNGKHIPLFNPGQTFAPSSVLMKEGRTEPPPYLSEADLIALMDHHGIGTDATISAHIKTIQAPAPDRQYAAK-NAQQRFIPSPLGVALSDGYTEMGHSLMNPALRAQMERDCAAIAAGTKQRQAVVAECLTHMKTVFEACTQQAIKLDEAMAEHFDA----IGTGNSTRVTRANFSKCGRCQQMMDLKPTGNGRRGGGGRLAYCRACTTGYPLPRNGRLEPHPHTCPLCGFQVLAVAQGKVRTAGYAV-PHCFNIAPGGRCGASSATGGLRCFMCSANCALA 690
            R W +C P++LF   +EK VRED   KQ+E+ LK EA KAQ LVLWLDCDREGENIA+EV +VC +V  +  L V+ +       F        H + H + Q  +    A +AR EIDLRLG+AFTRFQT R+Q   P      E+              V+S+G CQFPTLGF+V+R+  I++    P  + H            FTW R R++DHLACL LYE CL +  +A +T I  RDT K++P PL TVE  KRA+ +LR+ SE +   AE LY RG++SYPRTET  F   TDL  ++     H  WG Y + LL  G ++ PR G  DDQAHPPIHP K ++  +   SND E+++YE V  HFLACCS DA G  T V +++  E F A+GLM+ ERN+L+IY  +E+W    IP++  G  F P+ + +K G T PPP L+E+DLIA MD HGIGTDATI+ HI TI      RQYA K N   +F P+ LG+AL   Y  MG+ L  P LRA MERDC AIA G K    VV  C+  M+ +FE+  +++  LDE    +F +    + T NS RV   +F  CG+C + M+L+   +G     G L  C  C   Y LP +         CP+C F V+ + Q    + GY V P CFN  P  R         + C+ C+A+C  A
Sbjct:   70 RSWKSCDPVELFSAAVEKTVREDSTQKQIEKTLKSEASKAQLLVLWLDCDREGENIAFEVKSVCEKV--NRRLRVLRAR------FSALIPRDIHHAIHNLVQPNENLALACDARSEIDLRLGAAFTRFQTMRIQKKFPAVQNTSESR-------------VVSFGSCQFPTLGFVVDRYLAIESFVREPFYYLHVTHTLQDVGSIVFTWSRTRVYDHLACLALYETCL-DAQIATITSISRRDTSKRKPFPLTTVEFQKRAARWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLMALLALHTEHETWGGYVS-LLQNGKYEHPRSGKNDDQAHPPIHPTKSVSLSS--ISNDVERKVYEFVVLHFLACCSMDARGSQTNVTMQIAQETFSASGLMIRERNYLDIYR-YEKWAATVIPVYERGDQFVPTIMELKFGETSPPPLLTESDLIAKMDQHGIGTDATIAEHINTILK----RQYAIKMNNNTQFKPTELGLALVKSYDRMGYQLAQPDLRAAMERDCNAIAQGQKTCNQVVQSCMEQMRRIFESVVEKSRVLDETFQSYFGSAKPEVLTANS-RVVNPHFGMCGKCNKAMELRKLSDG-----GGLLTCAQCKGQYRLPIHATAVQTSTHCPICRFIVVEI-QKSDNSPGYMVCPFCFNEPPNPRLNE------MACYNCTADCPFA 726