prot_H-akashiwo_Contig1010.7.1 (polypeptide) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: D8LK58_ECTSI (Phenylalanine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK58_ECTSI)

HSP 1 Score: 457 bits (1175), Expect = 1.160e-157
Identity = 224/366 (61.20%), Postives = 271/366 (74.04%), Query Frame = 0

Query:    2 NNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRLAEEGLDPTKF--------VEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            NN+  SV  KIGK+LH    HPL IIK +IELYF  N  +L G P+F + DDLPPI +++ NFD+L I  DHVSR+P+DTYY++   VLR HTSAHQT+LL QG D FL CGDVYRRDEVD TH+PVFHQMEGVR+  E   P ++           DLK GLEG+A  LFG+ +MRW   YFPFT PS ELE+L++G W+EVLGCGVIH +V+ NAGR     E+GWAFGLGLERLAM+LF+IPDIRLFW++D RF SQFE   +  F+PYSKYPPC KDV++WLPE    H NDLFEV+R+VAGD+VE++ L+D+F HPKT R S+CFRITYR M RSLTNEEVD LQ  VR  +  +   ELR
Sbjct:   39 NNVSPSVAAKIGKDLHRCSAHPLGIIKERIELYFQKNTQAL-GQPQFKVFDDLPPIVTVEQNFDELLIPPDHVSRQPSDTYYVDAQRVLRCHTSAHQTSLLRQGEDRFLVCGDVYRRDEVDSTHYPVFHQMEGVRILTEEEAPARWDVSQRKEIASKDLKRGLEGLATNLFGNVEMRWVDAYFPFTTPSAELEILFRGKWMEVLGCGVIHDEVMDNAGRT---GEVGWAFGLGLERLAMVLFSIPDIRLFWSEDPRFSSQFEAKTLTTFQPYSKYPPCYKDVTFWLPEDNF-HNNDLFEVVRDVAGDLVEEIRLLDDFIHPKTQRKSRCFRITYRSMDRSLTNEEVDRLQEIVRGYITSRLDAELR 399          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A836CML8_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CML8_9STRA)

HSP 1 Score: 449 bits (1154), Expect = 3.570e-154
Identity = 223/373 (59.79%), Postives = 269/373 (72.12%), Query Frame = 0

Query:    2 NNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRD--AFLCCGDVYRRDEVDRTHHPVFHQMEGVRL--------AEEGLDPTKFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAP-----HQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            NN+P SV  KIG NLH R++HPLNIIKT+IE YF  +PA     P+ ++ D L P+ + ++NFD L I   HVSR+ TDTYY+++  VLRTHTSAHQ TLL +G    AFL CGDVYRRDEVDR+H+PVFHQMEGVR+             +       DL++GLEG+A  LFGD +MRW   YFPFT PSFELE+ + G WLEVLGCG I   V+R  G +  D  +GWAFGLGLERLAM+LF+IPDIRLFW+DD RF SQF+ G    FKPYSKYPPCLKDVS+W+P   A      H ND++E +REVAGDMVE+++L+DEF HPKTGR S+CFR+TYR M RSLTN+EVD LQ  VR  LV K GVELR
Sbjct:   49 NNVPASVAAKIGMNLHLRQHHPLNIIKTRIEDYFTQSPALREPRPQIEVHDSLAPVVTARDNFDSLLIPPSHVSRRATDTYYVDDKRVLRTHTSAHQVTLLRRGAPPRAFLVCGDVYRRDEVDRSHYPVFHQMEGVRVFAPDELPAGATAAEREALAVADLRAGLEGLACALFGDVEMRWVDAYFPFTNPSFELEIFFGGKWLEVLGCGAIERDVMRAGGMD--DATVGWAFGLGLERLAMVLFSIPDIRLFWSDDARFTSQFKAGAATTFKPYSKYPPCLKDVSFWVPGATAEIAHGFHDNDMYEAVREVAGDMVEEIALVDEFAHPKTGRVSKCFRLTYRSMDRSLTNKEVDALQELVRQRLVDKLGVELR 419          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A2D4CA88_PYTIN (Phenylalanine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CA88_PYTIN)

HSP 1 Score: 435 bits (1118), Expect = 1.000e-148
Identity = 214/364 (58.79%), Postives = 272/364 (74.73%), Query Frame = 0

Query:    3 NIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRLAEEGLDPT-------KFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            N+ +S+  KIG NLH RKNHPLNIIKTKIE YFN   +   G PKF I D+L P+ S  + FD + + +DHVSR+ TDTYY+N   VLR HTSAH+  ++ QG  AFL  GDVYRRDE+D +H+PVFHQMEGVR+  E LDP+       + V+ +LK  LEG+  ELFG+ +MRW   YFPFTEPS ELE+ +  DWLEVLG GV+  +++RNAG     +E+GWAFGLGLERLAM+LF+IPDIRLFW++D+RF SQF+ G+I +FKPYSKYP C KDVS+W  +    H+N+L EV+R++AGDMVEQV L+DEF HPKT R S+C+RITYR M R+LTN+EVD LQ  VR ++V + G+ELR
Sbjct:   62 NVTESIASKIGVNLHQRKNHPLNIIKTKIEGYFNKLHSD-HGAPKFQIFDNLSPVVSAHDCFDAMLVPKDHVSRRITDTYYVNPETVLRAHTSAHEVPMMKQGIKAFLISGDVYRRDEIDASHYPVFHQMEGVRIFSE-LDPSIPHEEKVEIVKEELKKTLEGMVHELFGEVEMRWVEAYFPFTEPSLELEIFFNNDWLEVLGSGVLRQEIVRNAGLG---EEVGWAFGLGLERLAMVLFDIPDIRLFWSNDERFISQFKDGEITKFKPYSKYPGCYKDVSFWHEDNF--HENNLCEVVRDIAGDMVEQVVLVDEFRHPKTQRQSKCYRITYRHMDRNLTNDEVDELQGLVREKMVAELGLELR 418          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A7S2ST50_9STRA (Phenylalanine--tRNA ligase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2ST50_9STRA)

HSP 1 Score: 429 bits (1103), Expect = 5.130e-147
Identity = 218/365 (59.73%), Postives = 261/365 (71.51%), Query Frame = 0

Query:   17 HNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRL--AEEGLDPTKFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFE-KGDIVEFKPYSKYPPCLKDVSYWLPETRAP-------------------HQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            H +  HPLN I+ +IE Y+            F    +LPPI S+++NFDDL I +DHVSR+P+DTYY+N+  VLRTHTSAHQ+TLL +G  AFL  GDVYRRDE+DR+H+PVFHQMEGV +  AE G D    VE DLK GLEG+ARELFGD +MRW   YFPFT+PSFELEV Y+GDWLEVLGCGVI   ++R AGR    +E GWAFGLGLERLAM+LF IPDIRLFW+ D RF SQF   G+IV+F+PYSKYPPC KDVS+WLPE                       H NDL EV+R+ A D+VEQ+ LID+FTHPKT R SQC+RIT+R M R+LTN EVD +Q QVR  +V + GVELR
Sbjct:   20 HTQPGHPLNTIRQRIEQYWKYRGFERGCDVDFFCASELPPIVSVQHNFDDLLIPKDHVSRRPSDTYYINDEYVLRTHTSAHQSTLLREGHSAFLATGDVYRRDEIDRSHYPVFHQMEGVLMFGAEVGADDP-IVEEDLKIGLEGMARELFGDVEMRWVDAYFPFTDPSFELEVFYEGDWLEVLGCGVIQQDIIRQAGRG---EERGWAFGLGLERLAMVLFGIPDIRLFWSKDPRFLSQFPVSGEIVKFEPYSKYPPCYKDVSFWLPEGAVVASSDGEVVDEAEASSGVVFHPNDLCEVVRDAAQDIVEQIELIDDFTHPKTQRRSQCYRITFRHMDRNLTNAEVDEMQLQVRRSMVARLGVELR 380          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A7S2WCK3_9STRA (Phenylalanine--tRNA ligase n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2WCK3_9STRA)

HSP 1 Score: 430 bits (1106), Expect = 6.850e-147
Identity = 206/360 (57.22%), Postives = 266/360 (73.89%), Query Frame = 0

Query:    2 NNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRL-AEEGLDPTKFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGD-MVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            NN+P++V + + K L  + +HPL I+K+KI  +F++      G  +F+  D L P+ S K  FDDL I +DH+SRKPTDT+Y++ +  LR HTSAHQ  L++ G  +FLC GDVYRRDE+D++H+PVFHQMEGV++   E    ++ VE DLK  LEG+ R +FGD +MRW   YFPFT+PS ELE+ +  +W+EVLGCGVIH  ++ N G     D+ GWAFGLGLERLAM+LF+IPDIRLFWTDD RFH+QF  G I +FKPYSKYPPCLKD+S+WLP+  A H NDLF  IR+ AGD +VE+V+LIDEFTHPKT R S C+RI+YR M RSLTNEE+D +Q  +R  + QK GVELR
Sbjct:   64 NNVPENVAKHVEKRLLQKPHHPLQILKSKIFEHFDTAEVERGG-VEFEKYDSLVPVVSTKQCFDDLLIPKDHISRKPTDTFYVDHDTCLRPHTSAHQIELITSGSRSFLCAGDVYRRDEIDQSHYPVFHQMEGVKIFPRESNVTSEQVEEDLKKSLEGLVRTIFGDVEMRWVEAYFPFTDPSIELEIWFNDEWMEVLGCGVIHQGIMDNCGLG---DQKGWAFGLGLERLAMVLFDIPDIRLFWTDDSRFHTQFTDGAITKFKPYSKYPPCLKDMSFWLPDNDAFHPNDLFSAIRDCAGDDLVERVTLIDEFTHPKTSRTSHCYRISYRSMDRSLTNEEIDTIQENIREHVTQKLGVELR 419          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A7S1ZPZ1_TRICV (Phenylalanine--tRNA ligase (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZPZ1_TRICV)

HSP 1 Score: 429 bits (1103), Expect = 9.530e-147
Identity = 215/382 (56.28%), Postives = 272/382 (71.20%), Query Frame = 0

Query:    3 NIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRL-------------AEEGLDPTKFVEHDLKSGLEGVARELFG-DCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDI-VEFKPYSKYPPCLKDVSYWL-------------PETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGV 356
            N+P  +  ++G+NLH    HPL+ IK+ I  +F+S P   +G P F+  DD+ P+ S  +NFD L I +DHVSR  +DTYYL++  VLRTHTSAHQTTLL++G D FL  GDVYRRDE+D TH+PVFHQMEGVR+              EEG++     E DLK  LEG+AR LFG D + RW  +YFPFT+PSFELEV++ G+WLEVLGCGV+H  +++NAG        GWAFGLGLERLAM+LF+IPDIRLFWTDD+RFHSQFE G + V F+PYSKYPPC KD+S+W+             P+    H+ND +EV+R+  GD+VE+V LIDEF HPKTGR S+C+RI+YR M RSLTNEEVD+LQ +VRT  V+  GV
Sbjct:   26 NVPPHIASRVGRNLHLTPGHPLHTIKSVIYDHFSSLP---NGCPPFETRDDMSPVVSTHHNFDSLLIPKDHVSRSKSDTYYLDDETVLRTHTSAHQTTLLAEGLDRFLVTGDVYRRDEIDGTHYPVFHQMEGVRIFTEEELAASGASTEEEGIE---LAERDLKLRLEGLARALFGSDVETRWVGEYFPFTDPSFELEVMHGGEWLEVLGCGVVHRDIIQNAGLGMRP---GWAFGLGLERLAMVLFSIPDIRLFWTDDERFHSQFEPGRVDVTFEPYSKYPPCFKDISFWIDEGMDDDDGSDDGPDAAPFHENDFYEVVRDAGGDLVERVELIDEFLHPKTGRTSKCYRISYRSMDRSLTNEEVDLLQERVRTGAVEGLGV 398          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A7R9U394_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U394_9STRA)

HSP 1 Score: 431 bits (1107), Expect = 1.090e-146
Identity = 213/367 (58.04%), Postives = 265/367 (72.21%), Query Frame = 0

Query:    1 MNNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRLAEE-----GLDPT---KFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            MNNI  ++ +++G++LH   NHPLNIIK +IE YF    A   G  +F++ DDL P+  +  NFDDL I EDHVSR P+DTYY+N+  +LR HTSAHQ TLL  G D FLC GDVYRRDE+D +H+PVFHQMEGVR+        G DP    K VE DLK  LEG+   +FG  + RW   YFPFTEPSFELE+ Y  +WLEVLGCGV+   +LR AGR    ++ GWAFGLGLERLAM+LF++PDIRLFW++D+RF  QF+ G+I +FK +SKYP C KDVS+W+ E    H NDL E++R V GD+VE+V LIDEFTHPKTG+ S  FRI YR M RSLTNEEV+ +Q++VR +LV  F VELR
Sbjct:   82 MNNISPAIVERVGRSLHRTPNHPLNIIKKRIEGYFVER-AKKDG-VEFEVFDDLAPVVKITQNFDDLLIPEDHVSRSPSDTYYINDELMLRGHTSAHQATLLQNGFDNFLCSGDVYRRDEIDASHYPVFHQMEGVRVFPHLEPAAGSDPVQGNKEVEADLKDALEGMVDAVFGPVEKRWIDAYFPFTEPSFELEIFYNDEWLEVLGCGVMQQDILRKAGRG---EQPGWAFGLGLERLAMVLFDVPDIRLFWSNDERFIKQFKDGEITKFKSFSKYPACYKDVSFWIDEETPFHPNDLAEIVRGVGGDLVEEVKLIDEFTHPKTGKTSNAFRINYRSMERSLTNEEVNEMQDRVRDQLVDAFKVELR 443          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A024TS89_9STRA (Phenylalanine--tRNA ligase n=11 Tax=Aphanomyces TaxID=100860 RepID=A0A024TS89_9STRA)

HSP 1 Score: 428 bits (1100), Expect = 2.290e-146
Identity = 211/364 (57.97%), Postives = 264/364 (72.53%), Query Frame = 0

Query:    2 NNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRLAEEGLDPTKF------VEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            NN+ +++  K+G NLH + NHPLNIIKTKI  YF+   A+  G PK  + D+L P+ S+ + FD + I EDHVSR+PTDTYYLN   +LRTHTS H+  L+ QG   FL  GDVYRRDE+D +H+PVFHQMEG+R   E      +      V+  LK+ LEG+   +FG  +MRW   YFPFT+PS ELE+ ++G+WLEVLGCGV+  Q+  + G     DE+GWAFGLGLERLAM+LF+IPDIRLFWT D RF SQF+ G I +FKPYSKYPPC KDVS+WLP     H+N+LFEV+R VAGDMVEQV L+D FTHPKT R S+CFRITYR + R+LTNEEVD +Q  VR  +V++ GVELR
Sbjct:   37 NNVSEAIASKVGMNLHRQPNHPLNIIKTKIATYFDDLHAN--GQPKMHLFDNLSPVVSVADCFDHMLIPEDHVSRRPTDTYYLNPTTLLRTHTSCHEVPLMKQGIKNFLVAGDVYRRDEIDASHYPVFHQMEGLRFFPELSRSIPYDARVALVQEHLKTTLEGMVESIFGKVEMRWVDAYFPFTDPSLELEIYFEGEWLEVLGCGVLQRQIAEHGG---VVDEVGWAFGLGLERLAMVLFDIPDIRLFWTTDARFLSQFQDGVISKFKPYSKYPPCYKDVSFWLPPEF--HENNLFEVVRNVAGDMVEQVVLVDAFTHPKTQRSSKCFRITYRHLDRNLTNEEVDDIQVVVRDAMVKELGVELR 393          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: W7TNG4_9STRA (Phenylalanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TNG4_9STRA)

HSP 1 Score: 429 bits (1104), Expect = 2.710e-146
Identity = 209/369 (56.64%), Postives = 264/369 (71.54%), Query Frame = 0

Query:    1 MNNIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRL----------AEEGLDPTKFVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            MNN+P ++  KIG++LH   +HPL IIK +IE YF    A+       +  D+L P+  + NNFDDL I  DHVSR P DTYY+++ +VLRTHTSAHQ+T+L +G +AFL  GDVYRRDE+DRTH+PVFHQMEGVR+           EEG+   K VE DLK  LEG+   LFGD + RW   YFPFT+PSFELE+ Y  +WLEVLGCGV+  +++R AG+    + +GWAFGLGLERLAM+LF IPDIRLFW+ D+RF  QFE G I  F P+SKYPPC KDVS+W+ +    + ND FE++R V GD+VE V L+DEF HPKTGR S+CFR+ YR M RSLTNEE+D+LQ +VRT++     VELR
Sbjct:   79 MNNVPVTLSGKIGRDLHRNPSHPLGIIKDRIEGYFKERAATTG--VAVECFDNLFPVVPIVNNFDDLLIPPDHVSRSPIDTYYVDDKQVLRTHTSAHQSTMLGKGLEAFLVTGDVYRRDEIDRTHYPVFHQMEGVRIFPGLLESRGSREEGV---KEVEKDLKEALEGMVVTLFGDVERRWVDTYFPFTDPSFELEIFYNDEWLEVLGCGVMQQEIVRRAGKG---ETLGWAFGLGLERLAMVLFQIPDIRLFWSTDERFTKQFEAGKITTFAPFSKYPPCYKDVSFWIDKVAGFNNNDFFEIVRGVGGDLVESVVLVDEFVHPKTGRASKCFRVNYRSMERSLTNEEIDVLQEEVRTQMSDALKVELR 439          

BLAST of mRNA_H-akashiwo_Contig1010.7.1 vs. uniprot
Match: A0A484EE71_BRELC (Phenylalanine--tRNA ligase n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EE71_BRELC)

HSP 1 Score: 428 bits (1100), Expect = 3.580e-146
Identity = 212/364 (58.24%), Postives = 267/364 (73.35%), Query Frame = 0

Query:    3 NIPDSVYQKIGKNLHNRKNHPLNIIKTKIELYFNSNPASLSGHPKFDIVDDLPPIASLKNNFDDLRIEEDHVSRKPTDTYYLNENEVLRTHTSAHQTTLLSQGRDAFLCCGDVYRRDEVDRTHHPVFHQMEGVRLAEEGLD---PTK----FVEHDLKSGLEGVARELFGDCKMRWNADYFPFTEPSFELEVLYQGDWLEVLGCGVIHPQVLRNAGRNRADDEIGWAFGLGLERLAMILFNIPDIRLFWTDDDRFHSQFEKGDIVEFKPYSKYPPCLKDVSYWLPETRAPHQNDLFEVIREVAGDMVEQVSLIDEFTHPKTGRHSQCFRITYRDMSRSLTNEEVDMLQNQVRTELVQKFGVELR 359
            NI  S+  KIG NLH ++NHPL+IIKTKIE YF+     L G PKF I DDL P+ +  + FD + + +DHVSRK TDTYY+++  VLR HTSAH+ T + +G  +FL  GDVYRRDE+D  H+PVFHQMEGVR+  E LD   P K    +V+ +LK+ LEG+A ELFGD +MRW   YFPFTEPS ELE+L+ G+WLEVLGCGV+  +++RNAG     + +GWAFGLGLERLAMILF IPDIRLFW+ D+RF SQF  G I  FKPYSKYP C KDVS+W  +    H+N+L EV+R++AGDMVEQV+++DEFTHP++ R S+C+RITYR M R+LTN E+D LQ  VR  +V    V LR
Sbjct:   50 NISQSIADKIGVNLHRQQNHPLHIIKTKIERYFDDQ-NRLHGSPKFTIFDDLDPVVTTYDCFDSMLVPQDHVSRKVTDTYYVDKYRVLRAHTSAHEVTTMKKGYTSFLISGDVYRRDEIDANHYPVFHQMEGVRIFTE-LDVFIPRKEKVVYVQQELKNTLEGIAIELFGDVEMRWVEAYFPFTEPSLELEILFNGEWLEVLGCGVLQQEIVRNAGLG---ENVGWAFGLGLERLAMILFGIPDIRLFWSGDERFTSQFRDGQITRFKPYSKYPACFKDVSFWHDDNF--HENNLCEVVRDIAGDMVEQVAVVDEFTHPQSQRKSKCYRITYRHMDRNLTNSEIDDLQKVVRARIVHSLNVVLR 406