mRNA_H-akashiwo_Contig972.4.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig972.4.1
Unique NamemRNA_H-akashiwo_Contig972.4.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: A0A7S3Y2D0_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y2D0_HETAK)

HSP 1 Score: 103 bits (257), Expect = 1.800e-27
Identity = 52/56 (92.86%), Postives = 52/56 (92.86%), Query Frame = 1 
Query:    1 AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSKRARDAGASAP 168
            AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSKRA  A A AP
Sbjct:   29 AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSKRATAADAPAP 84
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: A0A4D9D9L7_9STRA (tRNA-synt_His domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D9L7_9STRA)

HSP 1 Score: 58.2 bits (139), Expect = 1.830e-8
Identity = 27/43 (62.79%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:    7 AALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGS 135
            AA+ CE+  A  EPF +VHFD+ R HRGQMA A  LRL LEGS
Sbjct:  192 AAVACEVTHASLEPFQAVHFDMHRPHRGQMATAMNLRLLLEGS 234
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: W7TTH8_9STRA (Histidine ammonia-lyase n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TTH8_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 2.150e-8
Identity = 28/49 (57.14%), Postives = 33/49 (67.35%), Query Frame = 1 
Query:    7 AALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSKRARDA 153
            AA+ CE+  A  EPF ++HFD+ R HRGQMA A  LRL LEGS   R A
Sbjct:  204 AAVACEVTHASMEPFQALHFDMHRPHRGQMATAMNLRLLLEGSAMVRPA 252
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: A0A8K1C9F9_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C9F9_PYTOL)

HSP 1 Score: 52.4 bits (124), Expect = 2.040e-6
Identity = 25/46 (54.35%), Postives = 32/46 (69.57%), Query Frame = 1 
Query:    1 AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSK 138
            AVAALTCE+L+A + PF++   D+ R HRG +  A  LRL LE SK
Sbjct:  190 AVAALTCELLQASSVPFEAEFVDVSRPHRGIVTSATNLRLMLENSK 235
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: A0A835Z9D3_9STRA (AA_TRNA_LIGASE_II domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9D3_9STRA)

HSP 1 Score: 51.6 bits (122), Expect = 3.810e-6
Identity = 24/44 (54.55%), Postives = 30/44 (68.18%), Query Frame = 1 
Query:    7 AALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSK 138
            AAL+ +   A +EP    HFD+ RQHRGQM VAE +RL L GS+
Sbjct:  192 AALSLDACAAFSEPLQGAHFDVYRQHRGQMEVAERVRLLLTGSR 235
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: A0A2D4BVR0_PYTIN (Histidine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BVR0_PYTIN)

HSP 1 Score: 49.3 bits (116), Expect = 2.500e-5
Identity = 24/46 (52.17%), Postives = 32/46 (69.57%), Query Frame = 1 
Query:    1 AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSK 138
            AVAALTCE+++A + PF++ + D  R HRG +  A  LRL LE SK
Sbjct:  188 AVAALTCEVVQASSLPFEAEYVDASRPHRGIVTSATNLRLMLETSK 233
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BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Match: W4FK51_9STRA (Histidine--tRNA ligase n=10 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FK51_9STRA)

HSP 1 Score: 48.9 bits (115), Expect = 3.420e-5
Identity = 25/46 (54.35%), Postives = 29/46 (63.04%), Query Frame = 1 
Query:    1 AVAALTCEMLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSK 138
            AVAALTCE L+A   PF   H D  R HRG + VA  LR+ L+ SK
Sbjct:  193 AVAALTCEALQASPSPFQEAH-DSTRPHRGMLTVATHLRVMLDNSK 237
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig972.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
A0A7S3Y2D0_HETAK1.800e-2792.86Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A4D9D9L7_9STRA1.830e-862.79tRNA-synt_His domain-containing protein n=1 Tax=Na... [more]
W7TTH8_9STRA2.150e-857.14Histidine ammonia-lyase n=2 Tax=Nannochloropsis ga... [more]
A0A8K1C9F9_PYTOL2.040e-654.35Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A835Z9D3_9STRA3.810e-654.55AA_TRNA_LIGASE_II domain-containing protein (Fragm... [more]
A0A2D4BVR0_PYTIN2.500e-552.17Histidine--tRNA ligase n=1 Tax=Pythium insidiosum ... [more]
W4FK51_9STRA3.420e-554.35Histidine--tRNA ligase n=10 Tax=Aphanomyces astaci... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig972contigH-akashiwo_Contig972:54316..57239 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7G588
Preferred nameHARS
PFAMsHGTP_anticodon,WHEP-TRS,tRNA-synt_His
Model size168
Max annot lvl2759|Eukaryota
KEGG rclassRC00055,RC00523
KEGG koko:K01892,ko:K06675
KEGG ReactionR03655
KEGG Pathwayko00970,ko04111,map00970,map04111
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0000003,GO:0000122,GO:0000166,GO:0002119,GO:0002164,GO:0002181,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0003824,GO:0004812,GO:0004821,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0005829,GO:0006082,GO:0006139,GO:0006355,GO:0006357,GO:0006399,GO:0006412,GO:0006418,GO:0006427,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0007275,GO:0007276,GO:0007281,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009791,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010629,GO:0016070,GO:0016874,GO:0016875,GO:0017076,GO:0019219,GO:0019222,GO:0019538,GO:0019752,GO:0019953,GO:0022412,GO:0022414,GO:0030154,GO:0030554,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031974,GO:0032501,GO:0032502,GO:0032504,GO:0032543,GO:0032553,GO:0032555,GO:0032559,GO:0034641,GO:0034645,GO:0034660,GO:0035639,GO:0036094,GO:0042802,GO:0042803,GO:0043038,GO:0043039,GO:0043043,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0044703,GO:0045892,GO:0045934,GO:0046483,GO:0046983,GO:0048468,GO:0048519,GO:0048523,GO:0048609,GO:0048856,GO:0048869,GO:0050789,GO:0050794,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0051704,GO:0060255,GO:0065007,GO:0070013,GO:0071704,GO:0080090,GO:0090304,GO:0097159,GO:0097367,GO:0140053,GO:0140098,GO:0140101,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1902679,GO:1903506,GO:1903507,GO:2000112,GO:2000113,GO:2001141
Exons2
Evalue9.09e-05
EggNOG OGsCOG0124@1|root,COG2986@1|root,KOG0222@2759|Eukaryota,KOG1936@2759|Eukaryota
Ec32 ortholog descriptionHistidyl-tRNA Synthetase Similar to Histidine Ammonia-Lyase
Ec32 orthologEc-14_006630.1
EC6.1.1.21
DescriptionHistidyl-trna synthetase
Cds size144
COG categoryJ
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016,ko03036
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388063.952672-UTR-H-akashiwo_Contig972:54315..543391681388063.952672-UTR-H-akashiwo_Contig972:54315..54339Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig972 54316..54339 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388063.9678543-CDS-H-akashiwo_Contig972:54339..544031681388063.9678543-CDS-H-akashiwo_Contig972:54339..54403Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig972 54340..54403 +
1681388063.978779-CDS-H-akashiwo_Contig972:57159..572391681388063.978779-CDS-H-akashiwo_Contig972:57159..57239Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig972 57160..57239 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig972.4.1prot_H-akashiwo_Contig972.4.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig972 54340..57239 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig972.4.1

>prot_H-akashiwo_Contig972.4.1 ID=prot_H-akashiwo_Contig972.4.1|Name=mRNA_H-akashiwo_Contig972.4.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=48bp
MLEAPTEPFDSVHFDICRQHRGQMAVAETLRLFLEGSKRARDAGASAP
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mRNA from alignment at H-akashiwo_Contig972:54316..57239+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig972.4.1 ID=mRNA_H-akashiwo_Contig972.4.1|Name=mRNA_H-akashiwo_Contig972.4.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=2924bp|location=Sequence derived from alignment at H-akashiwo_Contig972:54316..57239+ (Heterosigma akashiwo CCMP452)
GCGGTGGCTGCTCTTACTTGCGAGATGCTGGAAGCCCCCACGGAGCCTTT
TGACAGTGTTCACTTTGACATTTGCCGGCAGCACAGAGGTAATTTCTTCA
GCAACATTCCAGAAATATATTGCAGGAGAATCAAACACTTTTCTTTGTTA
TTTTACATAAGACTATAGATGCATGTATATTTTGAGTATAGTGCTCGCAA
CCTTTCCTGCGTCATCCATTCCAGCATTTTCAAACAAGGAGGAGAAGAAG
GAGAGGGGAGAGGTGTAACCGTTTCCGtTTTTTGTCTTTTCCGCTATTGG
TGTTGTTGTGCTGCTGAACCACCTTCGTTACGATGGGACCTAGTTGGAGT
TTGGCCAACTTGGTGACTCTGTGAAGAAGGCATGACTTCATGTCGAAACG
TCGGGAGCTGGAGAGACGTATCATTTGCTAGAAGATTAATAATTAATTGT
TCCGTACCATCAGTGATCACTTTCATTTCAATATAATATATAATAATTAA
GGAGATCCTTCAGAATAAGACAAAAATAATGTGAGATGTTAGATTTTACA
GAAAAGCTACAAATATATGTATGGCTAGGTCTAACTACTAGGACCAGCAC
CTCGAGATGATCATCCTTGCTTGGCGCCAAAAAAAAAAAAAAAGAAGATA
ATGAAGAAATTACAACATATACGCGCTGCCGGAAAGTAGAAACGTTGGCA
ACCACCCTTCGTACAAAAACCTAAACATGAAATCCCAAACCTGAATCAAT
ACAAAACATACTATCCAAACAAGAATGCAGCACACCCCACTATCTCTGGA
AGATAACGACAATCCCCTCACCATCCTCGAACCCCGAGATCCTCCAGTTC
TTTCTGGAGcCCCTGGCCCGCAGATCCACCACCTTAATGAGCGTGAACTG
CACGCGGAcGCGCACTCCAGGCACTCAGTTAGTGGGACCGTTGAAAGAGG
CTTCTGCCATTTTGCCGAGAGCCGAGCAGCAATGCGCCTCAGGAAGGTCT
GCATGGACTGGTGGGCAATACCCTCCACAGTGACTATCAAGGGGCAGAAA
GTAGCACGGCGCTCTTCCGCAGCAGCCTTGTACCTCCTCACCTTTTCACG
GGCAGAGGTATCGAGGGCATTCTGGTAAGAGATGCGTTGGTTTGCACGTC
CAGGGCTGCCAGCATGTATATAATGCGGGTATCAAAAAGACAGTCCCTAT
CACATTTTTTGTTGTTGTATATATATGTAATATGATATATACCTAGGTAG
TTTTGGAAAATCAGTAACATGGCGCATTTTACAACTATATATATATTTAA
ATATAATCTTCCCTCCCGCAACTGTTACGCCTCTCCAGCTCCCTATGTTT
TGACATGAAATCGTGCCTTCTTCACAAGAGCCACAAGTATATGTACATCG
TTGTTTGTCATTCATTAAATAAATAATGGTATAGCGTAGTGTAATGTGGC
AATTCTAAAAACAAACCCTTTCATCAGAGCCCTCTTTTCACACCACCACC
CACACCCCGACAGCCTTTGGGGCCATCCATCAAATGACCACATCAATCAT
CAAGGGAAGGAGGGGGGTATGTGCAGCATCAGCATGATGGCTTTGTAGAA
ATAGAGGGAGAGGTTTTGTAAAAGGGGAGTTTAAGGGGATGGGCGATTTG
GGGACAGGGGGCTCAGAATTGACAAATATTACTCAAAGTTACCTCCTTCA
CGGTAGTACAAGAGGGACAAATGAGTTCCTGAAGAAGCAACTTTAATTCG
TAGCAGGGCGGCCGGCTTCACAAGACACGCAAGGGATTGCTGTCATGGTC
CAAAAAAAAAGAAAACGGCTACATTATTCTACAGATACTTCTAACGCTTA
GCTTGAGGTGCCTGCTCGGAGTGAACGCCTAATGAATTGCCTGATACAAC
AAGGGAATTTCAAAAGAGAGAGAAAGAAAAAGCAAAGAGGAATCAGTAAG
ACACCCAAGAGGTGTCTTTTGGCCTTTGGTCCGACTTTGGTTTTTTCTCC
CAAATGGTGTCACGCTAAAGTATACAAGAATTTGGCGAATAAAAAAAAAA
ACGTGTTCCGCTTTTGGGAAAATTGCGCTGAAAAGCTGAAACAGCAAATT
TGCATTTTCAGCTATTCACCGCAATTTTCCCAAGCGAAGTTTTTGAGATA
TATAGCTTAGATGAGGAGGCTGTTTTAGATTTTTTAGTCGAAGGTCATAT
GTTTTAAATCTTAACTGGCCGTGCGGCTAGAGCGCGCGGATTGCTTGTTT
TTTACGTCACCTCCGGGTTCGAGTCTGGGTTCGCAAAATTTGCCGTACTT
CCCATAGAGCGATGTCCCCTTTTGCCTGACATCAACTCCCCATCCGGAAA
GTTCTGGTAAAATACGATTGGTCAATCATTCATTCATTCATTCATTCATG
AAGAATGAAAACTCTTCCTGCATGCATATACCCAGCCTGCATTCATTTCC
ACCCGGGATTTTGACACATTGCATCAGCAAAAAATAGTCCGAGGAAGAGA
TGGAACAAATTGAATTACTGCTTTGCCCAGAATTTTCAAAAAAATAATAC
TTTACTGGCTCCCGCAGCATTTATTTTGATGAAATGATTTATTTCATCCC
TGATTTGTTTCATCCCAGGCAAAATAAACCAAGGGATGAAATACATTGTT
TTTTTCCATCATCCCTCTAGCGCTTTACAGAACAAGGGGCACACCACACA
AAACGATTCATTTCATTCCTAGAAATACTCCCAGATACCTCGAACTCGCA
GTTAAAAAGAACGACGCcaATAACAACCCCCCGCGGCCCCTTCCGGTGCC
CCTTCCGGTGCCCCCGCCGCCGCCGCCGCGCCGCCGCCGCGCAGGCCAGA
TGGCGGTCGCGGAGACCCTGCGGCTGTTCCTCGAGGGCTCAAAGCGCGCC
CGCGACGCGGGCGCCTCCGCCCCT
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Coding sequence (CDS) from alignment at H-akashiwo_Contig972:54316..57239+

>mRNA_H-akashiwo_Contig972.4.1 ID=mRNA_H-akashiwo_Contig972.4.1|Name=mRNA_H-akashiwo_Contig972.4.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=144bp|location=Sequence derived from alignment at H-akashiwo_Contig972:54316..57239+ (Heterosigma akashiwo CCMP452)
ATGCTGGAAGCCCCCACGGAGCCTTTTGACAGTGTTCACTTTGACATTTG
CCGGCAGCACAGAGGCCAGATGGCGGTCGCGGAGACCCTGCGGCTGTTCC
TCGAGGGCTCAAAGCGCGCCCGCGACGCGGGCGCCTCCGCCCCT
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