mRNA_H-akashiwo_Contig952.8.4 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A7S4DBJ3_HETAK (Methionine synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBJ3_HETAK)

HSP 1 Score: 2518 bits (6526), Expect = 0.000e+0
Identity = 1271/1276 (99.61%), Postives = 1274/1276 (99.84%), Query Frame = 1

Query:    1 ETKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            ETKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCP+AKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFME EKAAKLAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGG +SLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEA+RHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD
Sbjct:   11 ETKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEAEKAAKLAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGHSSLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEAARHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 1286          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A7S2XWE9_9STRA (Methionine synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XWE9_9STRA)

HSP 1 Score: 2003 bits (5190), Expect = 0.000e+0
Identity = 998/1273 (78.40%), Postives = 1113/1273 (87.43%), Query Frame = 1

Query:   10 GSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            G+VENYPYFNRE DSV+AYL RLFQE+I +YDGAMGTMIQKYKL EE+YRGE+FKD+H LVKGNNDMLS+TQP+IIKEIH +YL EG SQLIGTNTFS TTIAMADY+ME++V ++N  S R+ARE  DEVTA DPTRPRFVVGAIGPTNRTGSISP+VED S RNVTFDELE AYY+Q   L+EGG D+++VETIFDTLNAKAA+FA NR+ EE G  + PLF+SGTLVDQSGRTLSGQT EAFY S+RH KPFCVGLNCALGA QMKPFLRRLA V ECFVHVYSNAGLPNAMGGYDDTP+DMAREN IFA E+LVNMIGGCCGSTPPHI AI+  V+GMPPR LP   EP +WLSGLEDL+VSKDRF+FLNVGERCN+AGSI FKK I+ GDY  AM+IAKKQVEDGAMV+D+NVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAG+KWVQGKSI+NSISLKVGEEEFKR A I K+HG A++VMAFDE GQAATE +KV +CKRSYDILV+EV FPPEDIIFDPNILT+ATG+EEHNNYGVDFI AT++IKE CPYA+ISGGVSNLSFGFRGVNVIREA+HSVFLYHAVAAGMDMGIVNAGMMEVYDDIP DLL LVEDAVLNR  +ATE+LL+RSL E+EK A+ GG   K VEWR +PV ERLT+ALIKGI ++ID DVEECRQ V +PL+VIEGPLM GMN+VGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEK A L A+G+     D+DSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVM  CE IL   ++Y+ DV+GLSGLITPSLDEMVFVAKEM K GLKQPLMIGGATTSKMHTAVKI+PQYA  EHPVIHVLDASRSVTVVQSLL    K+ +VE+L+EEYE+LREEHYA LEER +L   + + KGLQ+DFAA PPV AP   GV  L+ Y L DV+P+IDWNPFFQTWELRGRYPNRGYPKIFNDE VG EA+KLFD+AQ ML+DIV NK+L L GV  IF A R   GEDVEV++         KFCMLRQQAEKE++ PYLS+ADF+AP+  G  + D++G FAVAC+GA+EEA+R EA NDDY+KIMVQALADRLVEAFAE LHRD+R ++WGYAPDE+LPAEDLHKIRYQGIRPAPGYPSQPDHTEK TMW+LL+VEE SGIRLSE+LSM+PASSVSAL FAHP A+YFAVGKI +DQV EYA RKGM+V EMEKWLSPILNYDR+
Sbjct:   11 GTVENYPYFNREKDSVRAYLTRLFQEEIAIYDGAMGTMIQKYKLGEEEYRGERFKDYHTLVKGNNDMLSITQPQIIKEIHLKYLEEGNSQLIGTNTFSSTTIAMADYDMESYVRELNVESVRLAREACDEVTAKDPTRPRFVVGAIGPTNRTGSISPNVEDPSVRNVTFDELEIAYYEQVCGLVEGGCDILMVETIFDTLNAKAAVFACNRFLEEHGLEDMPLFVSGTLVDQSGRTLSGQTTEAFYVSIRHCKPFCVGLNCALGATQMKPFLRRLAVVAECFVHVYSNAGLPNAMGGYDDTPDDMARENLIFADEKLVNMIGGCCGSTPPHIKAISEAVKGMPPRPLPEKKEPVLWLSGLEDLVVSKDRFTFLNVGERCNIAGSIRFKKLILGGDYATAMDIAKKQVEDGAMVLDINVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGMKWVQGKSIINSISLKVGEEEFKRQAGIIKRHGAAVIVMAFDEEGQAATESEKVRICKRSYDILVNEVQFPPEDIIFDPNILTIATGIEEHNNYGVDFIEATKKIKEICPYARISGGVSNLSFGFRGVNVIREAIHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPKDLLQLVEDAVLNRHSEATEKLLERSLEEKEKAASGGGPKKKEVEWRIKPVTERLTYALIKGISQYIDEDVEECRQTVDRPLQVIEGPLMQGMNIVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKKAALEAKGITEMPEDDDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMQPCESILDAIKKYQPDVVGLSGLITPSLDEMVFVAKEMEKAGLKQPLMIGGATTSKMHTAVKISPQYANNEHPVIHVLDASRSVTVVQSLLG-DDKDEFVEDLMEEYEDLREEHYAGLEERCFLALDQAKQKGLQIDFAACPPVQAPNQLGVTVLDSYTLEDVLPYIDWNPFFQTWELRGRYPNRGYPKIFNDENVGGEAKKLFDEAQEMLKDIVANKRLHLKGVCGIFAANRV--GEDVEVYTGADKSEVAAKFCMLRQQAEKESEAPYLSMADFIAPKDSG--LDDYMGMFAVACMGAEEEAARFEAENDDYSKIMVQALADRLVEAFAEALHRDMRVSIWGYAPDEKLPAEDLHKIRYQGIRPAPGYPSQPDHTEKTTMWNLLQVEEKSGIRLSEALSMLPASSVSALCFAHPQAEYFAVGKIEKDQVVEYAERKGMEVAEMEKWLSPILNYDRE 1278          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A097PA47_9STRA (Methionine synthase n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A097PA47_9STRA)

HSP 1 Score: 1820 bits (4713), Expect = 0.000e+0
Identity = 927/1276 (72.65%), Postives = 1062/1276 (83.23%), Query Frame = 1

Query:   22 NYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTET-PLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVVKA-VEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAP-RAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEG----GQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            +YP F R  DS  AY  +LF+E+IV+YDGAMGTMIQK++L+EEDYRGE+FKD+HKLV+GNND+L+LTQP IIK+IHR+YL +G + LIGTNTFS TTIA ADY+ME+   ++N  SARIAREVADEV+A +P RPRFV G+IGPTNRT SISP V DAS+RNVTFDEL+ AYY++   L++GG D II+ETIFDTLNAKAAIF+V R  +E G  ET PL ISGTLVDQSGRTLSGQT EAFY SVRH  PFCVGLNCALGA QM+PFL+RL+ V ECFV VYSNAGLPNAMGGYDDTP DMA +N +F  E LVNMIGGCCGSTP HI AIA   EG+PPR +    +  MWLSGLEDL+VSK+RF FLNVGERCN+AGS LFKK I+AGDY  AM+IAKKQVEDGAMV+DVN+D+G+LDG+AAMQ+FLKIA+TEPE SKVP M+DSSKFEI+EAGLKWVQGK IVNSISLKVGEEEFKR A + K HG A+VVMAFDE GQAA E +KV +CKRSYDILVDEVGFPPEDI+FDPNILT+ATG+EEHNNY VDFI A R IKE CP+AKISGGVSNLSFGFRGVNVIREAMHSVFLYHAV AGMDMGIVNAGM++VY DIP DLL +VEDAVLN+  +ATE LL+RS+VEREK  A  G+       WRT  V  RLTHAL+KGIDEFID DVEECRQ V +PLEVIEGPLM+GMNVVGDLFG+GKMFLPQVIKSARVMKKAVAYLLPFME+EK  K+  QG++   AD+DS YAG VL+ATVKGDVHDIGKNIV VVLGCNNYKV+D+GVM  CE I+ KA E K  VIGLSGLITPSLDEMVFVAKEM+K G+  PL+IGGATTSKMHTAVKIAP Y+T EHP IHVLDASRSVTVV SLL    KE YV+++L+EY+ELREEHYA LEER    F   +  G  +DF A PPV +P    GV+ ++ Y L DVVPFIDWNPFFQTWELRG+YPNRGYP+IFNDE VG EA+KLF DAQA+L+D+VDNK+L L GV  +FPAAR   GEDVEV++ E     G+ ++GKFCMLRQQ EKE+D+ Y SLADFVA +  G   +D+LGAFAVAC GA+EE  R+EA NDDYNKIMVQALADR VEAFAE LHR++R   WGYAP+EEL  EDL KI+Y GIRPAPGYPSQPDHTEK  MW+LLK  EL+GI L+ESLSM PASSVSALVFAHP++QYFAVG+I +DQV++YA RKGM V E+E+WL+PILNYDRD
Sbjct:   13 DYPRFVRGRDSTLAYFHKLFRERIVIYDGAMGTMIQKHRLTEEDYRGERFKDYHKLVRGNNDLLTLTQPTIIKDIHRKYL-KGGADLIGTNTFSSTTIAQADYDMESLAYELNVESARIAREVADEVSAEEPERPRFVGGSIGPTNRTASISPDVVDASKRNVTFDELDEAYYEEARGLIDGGCDFIIIETIFDTLNAKAAIFSVRRLLKERGMEETMPLIISGTLVDQSGRTLSGQTTEAFYVSVRHCNPFCVGLNCALGAKQMRPFLQRLSKVAECFVSVYSNAGLPNAMGGYDDTPADMAADNSVFVKESLVNMIGGCCGSTPDHINAIAKMCEGVPPRPVHERVKH-MWLSGLEDLVVSKERFQFLNVGERCNIAGSPLFKKLILAGDYAKAMDIAKKQVEDGAMVLDVNMDEGLLDGLAAMQRFLKIAMTEPECSKVPIMVDSSKFEIIEAGLKWVQGKCIVNSISLKVGEEEFKRQAAVVKAHGAAVVVMAFDEEGQAADEYNKVRICKRSYDILVDEVGFPPEDIVFDPNILTIATGIEEHNNYAVDFIKACRTIKEVCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVQAGMDMGIVNAGMLQVYSDIPPDLLQIVEDAVLNKHAEATEALLERSMVEREKALANKGQATAVQAPWRTLEVQARLTHALVKGIDEFIDEDVEECRQLVPRPLEVIEGPLMSGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPFMEKEKEEKMKEQGIEAV-ADDDSAYAGVVLLATVKGDVHDIGKNIVGVVLGCNNYKVYDMGVMIPCEKIIEKAIEVKAQVIGLSGLITPSLDEMVFVAKEMSKAGMTVPLLIGGATTSKMHTAVKIAPNYSTVEHPCIHVLDASRSVTVVGSLLGED-KEEYVQDVLDEYDELREEHYAGLEERHLKTFDAAKKLGFNIDFGASPPVCSPVGGTGVKVVDYYSLEDVVPFIDWNPFFQTWELRGKYPNRGYPRIFNDENVGEEAKKLFADAQALLKDLVDNKRLSLRGVVGVFPAARV--GEDVEVYASEEAKDRGEEAIGKFCMLRQQQEKESDDAYFSLADFVASKESGH--KDYLGAFAVACFGAEEEVERYEALNDDYNKIMVQALADRFVEAFAEALHREMRMKTWGYAPEEELDPEDLLKIKYDGIRPAPGYPSQPDHTEKVMMWELLKARELAGIGLTESLSMTPASSVSALVFAHPESQYFAVGRIEKDQVEDYAVRKGMVVEEVERWLAPILNYDRD 1280          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A836CPG7_9STRA (Methionine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CPG7_9STRA)

HSP 1 Score: 1809 bits (4685), Expect = 0.000e+0
Identity = 916/1276 (71.79%), Postives = 1044/1276 (81.82%), Query Frame = 1

Query:   13 SVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPT-LGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAA--AGGKV-VKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDPQE-ADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETD-EPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYD 3822
            SV +YP + +  DS  AYL RLFQE+IVMYDGAMGTMIQK++L EEDYRGE+FKD+ KLV+GNND+LS+TQP+II++IH +YL  G S +IGTNTFS TTIA ADY ME  V ++N+ SA++AR   DEVTA DP+RPRFV G+IGPTNRT SISPSVED S RNV FDEL  AYY +   L+EGGSD+IIVETIFDTLNAKAA+FAV +Y  ET   + PL ISGTLVDQSGRTLSGQTGE FY S+RH KPFC+GLNCALGA QMKPFLRRL+ V ECFVHVYSNAGLPNAMGGYDDTP DMAR+NH FASE +VNMIGGCCGSTP HI A+  DV+   PR LP  L  P MWLSGLE L VSKDR +FLNVGERCN +GSI FKK IMAG+Y +AM++AK QVEDGAMV+D+N+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKFEI+EAGLKWVQGK IVNSISLKVGE EF+R ATI + HG A+VVMAFDE GQAA E+ KV +CKRSYDILVDE+ FPPEDIIFDPNILT+ATG++EHNNY +DFINATRRIKE CP+ KISGGVSNLSFGFRGVN IREAMHSVFLYHA+ AGMDMGIVNAGM++VYDDI  DLL +VEDAVLN+ P ATE LL+R+ +ERE   A  AGGKV VK   WR +PV  RLTH+LIKGID++I+ D EE R    +PL VIEGPLM+GMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEK  K   QG+DP E  D+D+ YAGKV++ATVKGDVHDIGKNIV+VVLGCNNYKV+D+GVM  CEDIL    +YK D++GLSGLITPSLDEMVFVA+EMAK G  QPLMIGGATTSKMHTAVKIAPQY++ EHPVIHVLDASRSVTVV SLL   +K  Y EE+LEEYEELRE               K   K L+++F A P    P   G + L+ Y L  V+ +IDWNPFFQTWELRGRYPNRGYPKIFND  VGAEA+KLFDDAQ ML DIV NK+LRLTGV  ++PA R   GEDV ++S       L  FCMLRQQAEK+T  E YLSLADFVAP G     RDHLG FAVAC+G D+E  R EA NDDY+KIMVQALADRLVEAFAE LH D+R  +WG+A +E++  EDL K++YQGIRPAPGYPSQPDHTEKRTMW++LK EEL+GI+LSESLSMMPASSVSALVFAHP ++YFAVGKIG+DQV+ YAARKGM + E ++WL PILNYD
Sbjct:   14 SVADYPRYTKGKDSCTAYLTRLFQERIVMYDGAMGTMIQKHRLEEEDYRGERFKDYDKLVRGNNDLLSITQPQIIRQIHIEYLESG-SDIIGTNTFSSTTIAQADYGMENLVYELNYESAKLARSACDEVTARDPSRPRFVAGSIGPTNRTASISPSVEDPSYRNVNFDELVDAYYFEAKALVEGGSDIIIVETIFDTLNAKAAVFAVRKYLRETNQEDIPLMISGTLVDQSGRTLSGQTGEGFYVSMRHVKPFCIGLNCALGAQQMKPFLRRLSMVAECFVHVYSNAGLPNAMGGYDDTPSDMARDNHEFASEGMVNMIGGCCGSTPQHIKAMHEDVKDCAPRPLPKPLDPPVMWLSGLEPLQVSKDRINFLNVGERCNCSGSIQFKKLIMAGNYSSAMDVAKAQVEDGAMVVDINLDDGLLDGLAAMQKFCKIAVTEPDVSKVPFMIDSSKFEILEAGLKWVQGKCIVNSISLKVGETEFRRQATIVRDHGAAVVVMAFDEEGQAADEESKVRICKRSYDILVDELKFPPEDIIFDPNILTIATGIDEHNNYAIDFINATRRIKEVCPHCKISGGVSNLSFGFRGVNTIREAMHSVFLYHAIQAGMDMGIVNAGMLQVYDDIAPDLLKIVEDAVLNKHPGATEALLERAQIERELADARKAGGKVAVKEGSWRDQPVKARLTHSLIKGIDQYIETDTEEARSQADRPLSVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKRLKALEQGVDPNEPVDDDAAYAGKVVIATVKGDVHDIGKNIVAVVLGCNNYKVYDLGVMVSCEDILKAVDKYKPDIVGLSGLITPSLDEMVFVAREMAKKGCTQPLMIGGATTSKMHTAVKIAPQYSSPEHPVIHVLDASRSVTVVSSLLGG-EKTEYAEEVLEEYEELREXXXXXXXXXXXXTLEKAMGKRLEINFEATPAPQPPSFIGNKVLDNYSLESVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDATVGAEAKKLFDDAQVMLNDIVANKRLRLTGVLGMYPANRV--GEDVVLYSDASRAEKLATFCMLRQQAEKDTPTEAYLSLADFVAPVGH----RDHLGMFAVACMGCDDEVKRFEAENDDYSKIMVQALADRLVEAFAEALHADVRREIWGFASEEKVAGEDLFKLKYQGIRPAPGYPSQPDHTEKRTMWNVLKAEELAGIQLSESLSMMPASSVSALVFAHPQSEYFAVGKIGKDQVESYAARKGMPLDECQRWLGPILNYD 1281          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: D7FUT0_ECTSI (Methionine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUT0_ECTSI)

HSP 1 Score: 1762 bits (4564), Expect = 0.000e+0
Identity = 892/1227 (72.70%), Postives = 1021/1227 (83.21%), Query Frame = 1

Query:  172 KDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGK-VVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAV----AKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDPQEADE--DSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEG-YELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            +D+H LVKGNNDMLS+TQP IIKEIH +Y+  G S +I TNTFS TTIAMADYEME  V ++NF SAR+ARE  +E TA DPT+PRFV+GA+GPTNRTGSISP VED S RNVTFDEL  AY  QT  L++GG+D+IIVETIFDTLNAKA +FAV  YF+E G T+ PLF+SGTLVDQSGRTLSGQT E FY S+RH KPFC+GLNCALGA+QM+PFL+RLA V ECFVHVYSNAGLPNAMGGYD+TPEDMAR+N +FA E L+NM+GGCCGSTP HI A+   VE    R LP   EP MWLSGLEDLIV+K+RF FLNVGERCN++GSI FKK I+ GDY  AM++AK+QVEDGAMVID+N+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKF IVEAGLKWVQGKSIVNSISLKVGEEEFKRHA I K HG A+VVMAFDE GQAA  D+KV +CKRSYD+LVDEVGFPPEDIIFDPNILT+ATG++EHNNY VDFI AT RIKE CPY KISGGVSNLSFGFRGVNVIRE+MHSVFL+HAV AGMDMGIVNAGM+EVYDDI   LL +VEDAV NR   ATE LLD + +E+EK     GK  V+A  WRT+ V ERLTH+LIKGID+++  DVEECR  +     +PL+VIEGPLM+GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK AK+ A G+DP   D+  DS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYKV+D+GVM  C+ ILAKA++ KVDVIGLSGLITPSLDEMVFVAKEMAK G K PL+IGGATTSKMHTAVKI+PQYA+ E PVIHVLDASRSVTVV +LLS  +K  YVEE++EEYEELREEHYA  EER YL F K  +K   +DF  RPPV  P  PG+  + G YEL DV+ +IDWNPFFQTWELRGRYPNRGYPKIFNDE+VG EA+KLF++AQ ML+ IV  K L L G+ AIFPAA T DGEDVEV++ +  +  L +FCMLRQQAEKE+D+PYLS ADF+AP+  G  V D++G FA+AC+G ++     E  NDDY+KIMVQAL DR VEAFAEKLH D+R  +WGYAPDE++  +DL K++YQGIRPAPGYPSQPDHTEKRTMWDLL   EL+GI LSESLSMMPASSVSALVFAHPD++YFAVG I +DQV  YA RKGMD+   EKWLSPIL+YDRD
Sbjct:    3 QDYHILVKGNNDMLSITQPHIIKEIHTKYVDSG-SDIIATNTFSATTIAMADYEMEKDVYELNFESARLAREACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGADIIIVETIFDTLNAKAGVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAEEGLINMLGGCCGSTPAHIKAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQERLTHSLIKGIDQWVIEDVEECRLMLDDQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDLILAKAKQLKVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLS-DRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKNIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVAIFPAA-TVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESG--VADYMGMFAIACMGCEKMVKEFEDLNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1224          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A7S3JTL8_9STRA (Methionine synthase n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JTL8_9STRA)

HSP 1 Score: 1757 bits (4551), Expect = 0.000e+0
Identity = 890/1294 (68.78%), Postives = 1051/1294 (81.22%), Query Frame = 1

Query:   19 ENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDR----FSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDE-VGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHA-VAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGK-------VVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVA----KPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQGLDP--QEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPR-APGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGK----FCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            E +PYF R  DSV+ YL +LFQ +I MYDGAMGTMIQK+KL EED+RGE+FKD+H L+KGNND+LS+T+P+II  I+ +YLVEG SQLIGTNTFS TTIA ADY+M++ + ++N+ SAR+AREV D+VTA DPT+PRFVVGAIGPTNRT SISP V D + RN TFDEL  AYY+Q V L++GG+D++IVETIFDTLNAKAA+FAVN Y E   N + PL ISGTLVDQSGRTLSGQTGEAFYAS+RHAKP CVGLNCALGA+ M PFL+RLA+  ECFVHVYSNAGLPNAMGGYDDTP+DMAREN +FA    VNMIGGCCGSTPPHIAAI   ++   PR LPT   P MWLSGLED +V          F+NVGERCN+AGS  FK+ +M G +  AM+IAK QVE+GA ++DVNVDDGMLDG+ AMQKF+K+AVTEP++SKVPFMIDSSKFEIVEAGLKW QGK IVNSISLKVGEE+FK HA   +KHG A+VVMAFDE GQAA+ +DKV +CKRSYDILV E + +PPEDI+FDPNILT+ TG+EEHNNYGVDFI A R IK+ CP+AKISGGVSNLSFGFRGVN+IRE++H+VFL+HA + +GMDMGIVNA  M    ++P  L  +  DAV+N RPDATERLL  +  E EK+AA   K       V K   WR  P+ ER+TH+L+KGI EF+D D EE R  +A    +PLEVIEGPLM GMNVVGDLFG+GKMFLPQVIKSARVMKKAVAYLLP+ME EK   L AQG+DP  Q+ D+DS YAGK L+ATVKGDVHDIGKNIV+VVLGCNNYKV+DIGVMC+C+ IL KA+EYKVD IGLSGLITPSLDEMVFVAKEM+K GL+ PL+IGGATTSKMHTAVKIAPQYA+ EHPVIHVLDASR+VTVV SLL+A  K  YV ++ +EY ELR+E+YASLE+R +L   + +AK  +++++  PP  AP+ A G   +  YEL+DVVP IDWNPFFQTWELRGRYPNRGYPKIFNDE VGAEA++LFDDAQ M++ IVD K+L L GV  ++ A R+E GEDV+V+  +  + +  K    FCM RQQAE E+   Y+S ADF+APRG   A +D+LG FAVAC GA+E A+++EA NDD++KIM+QALADRLVEA AE LHRD+RT  WGYA DE L AEDL K++Y+GIRPAPGYPSQPDHTEKRTMWDLL VE  SGI+LSES+SMMPASSVSALVFAHP+A YFAVGKI +DQV+ YA RKG  +PEME+WLSP+LNYDRD
Sbjct:   83 EGFPYFVRGRDSVREYLTKLFQTRITMYDGAMGTMIQKHKLEEEDFRGERFKDYHMLIKGNNDLLSITKPDIIGGIYEKYLVEGGSQLIGTNTFSSTTIAQADYDMQSLIYELNYDSARLAREVCDKVTAQDPTKPRFVVGAIGPTNRTASISPDVNDPTARNCTFDELVEAYYEQCVGLVDGGADILIVETIFDTLNAKAAVFAVNEYLEHA-NVDIPLIISGTLVDQSGRTLSGQTGEAFYASIRHAKPICVGLNCALGASAMAPFLKRLADCAECFVHVYSNAGLPNAMGGYDDTPQDMARENLMFAENGWVNMIGGCCGSTPPHIAAIQEAIKDCKPRPLPTRKAPRMWLSGLEDFVVESAHNNVGLPFVNVGERCNIAGSRKFKRLVMEGKFAEAMDIAKDQVENGAHIVDVNVDDGMLDGMNAMQKFIKMAVTEPDVSKVPFMIDSSKFEIVEAGLKWCQGKPIVNSISLKVGEEKFKEHALCLRKHGAAVVVMAFDEQGQAASREDKVRICKRSYDILVSEPIYYPPEDIVFDPNILTIGTGIEEHNNYGVDFIAAVREIKQVCPWAKISGGVSNLSFGFRGVNIIRESIHAVFLHHACLDSGMDMGIVNAKEMLALSEVPKHLRGVCIDAVMNTRPDATERLL--TATEAEKEAAEARKRGGGDVAVAKTTTWRDMPIEERITHSLVKGISEFVDKDTEELRAEIAARGGRPLEVIEGPLMAGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPYMEAEKRENLIAQGIDPDSQQQDDDSQYAGKFLIATVKGDVHDIGKNIVAVVLGCNNYKVYDIGVMCECDKILEKAKEYKVDAIGLSGLITPSLDEMVFVAKEMSKKGLQVPLLIGGATTSKMHTAVKIAPQYASDEHPVIHVLDASRAVTVVSSLLNADDKPDYVADIADEYAELRDEYYASLEDRKFLTLEQAQAKRCELNWSKVPP--APKTALGAHTIT-YELSDVVPLIDWNPFFQTWELRGRYPNRGYPKIFNDETVGAEAKRLFDDAQKMMKQIVDEKRLELRGVYGLYAAQRSEGGEDVDVWDSDAARDAKEKPKATFCMFRQQAETESAAAYMSQADFIAPRG---AAKDYLGMFAVACFGAEEWANKYEAQNDDFSKIMMQALADRLVEACAEALHRDMRTKYWGYASDETLSAEDLIKVKYEGIRPAPGYPSQPDHTEKRTMWDLLDVESRSGIKLSESISMMPASSVSALVFAHPEATYFAVGKIEKDQVKSYAERKGYSLPEMERWLSPMLNYDRD 1367          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A8K1C6F4_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C6F4_PYTOL)

HSP 1 Score: 1753 bits (4541), Expect = 0.000e+0
Identity = 884/1275 (69.33%), Postives = 1043/1275 (81.80%), Query Frame = 1

Query:   28 PYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAA--GGKVVKA---VEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAK---LAAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQ-KEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDR 3825
            P+   ETD + AYL  L +++I+++DG MGTMIQ++KL+EED+RGE+FKDF  LVKGNND+LS+T+P+IIK+IHR+Y+  G +QLIGTNTFSGTTIA ADY+ME  V ++N+ SA+IAREVADEVTA DP +PRFV G+IGPTNRT SISP+VED   RNVTFDEL AAYYQQ   L++GGSD+++VETIFDTLNAKAA++A+++Y E TG    PLFISGT+VD SGRTLSGQT EAFY S+RH+KPFC+GLNCALGA QMKPFL+RL+NV ECFV VY+NAGLPNAMGGYDD+P+ MA     F  + L+NM+GGCCG+TP HI A+A        R LP L +P MWLSGLEDL+V+K+RFSFLN+GERCN++GSI FKK I+ GDY  AM+IA++QVEDGAMVIDVNVDDGMLDGVAAM++FL IAVTEP++SKVPFMIDSSKF IVEAGLK VQGK IVNSISLKVGEE F  HA I K+HG A+VVMAFDE GQAATE++K+ +CKRSYDILV++VGFPPEDIIFDPNILT+ATG+EEHNNYGVDFINATRRIKE CPYAKISGGVSNLSFGFRGVNVIREA+HSVFLYHAVAAGMDMGIVNAGM+EVYDDIP DLL LVED VLNR   ATE LLDRSL+E+EK  AA  GG    A    EWRT+P+ ERLTHAL+KGI EFID DVEE R+A A+PL+VIEGPLM+GMNVVGDLFG+GKMFLPQVIKSARVMKKAVAYLLPFMEEEKA      AA G   +E D+DS YAGKVLMATVKGDVHDIGKNIV VVLGCNNYK+ D+GVM  CEDIL  A+E KVD+IGLSGLITPSLDEMV VA+EM K GLK PLM+GGATTSKMH AVKIAP Y+T +HPVIHVLDASRSV VV +LL   + +E +VE+LLEEYEELREE+YASL++   + FA  + K  Q+DF   PP       G   +E  +L  +VPFIDWNPFFQTWELRGRYPNRGYPKIF+DE+VG EARK+FD+ Q +L++I+  K + + GV+ IFPA R  +GEDV V  P      + KFCMLRQQAEKET +PY+SLADFVAPR  G    D++G FAV   G +E A++ E+ +DD+NKIM QA+ DRL EAFAE +HR++R   WGYA +E L  EDL K++Y GIRPAPGYPSQPDHTEKR +W+LLK EEL+G+ L++S  M+P S+VSAL FAHP++QYFAVGK+ ++QV EYA RK   + E EKWL+PIL YDR
Sbjct:   18 PFVRGETD-IFAYLTALMKQRILIFDGGMGTMIQQHKLTEEDFRGERFKDFPMLVKGNNDLLSITRPDIIKDIHREYMEVGLAQLIGTNTFSGTTIAQADYKMEHLVYELNYESAKIAREVADEVTAKDPLKPRFVAGSIGPTNRTLSISPNVEDPGFRNVTFDELVAAYYQQVEGLVDGGSDILLVETIFDTLNAKAAVYAIDQYQENTGR-RLPLFISGTIVDMSGRTLSGQTTEAFYVSLRHSKPFCIGLNCALGANQMKPFLQRLSNVAECFVSVYANAGLPNAMGGYDDSPQLMADHCEEFCKDYLINMMGGCCGTTPKHIRAMAEMTMNYTARPLPPLKDPFMWLSGLEDLVVTKERFSFLNIGERCNISGSIRFKKLILKGDYGTAMDIARQQVEDGAMVIDVNVDDGMLDGVAAMERFLNIAVTEPDVSKVPFMIDSSKFHIVEAGLKCVQGKCIVNSISLKVGEELFCEHARIVKRHGAAVVVMAFDEEGQAATEEEKIRICKRSYDILVEKVGFPPEDIIFDPNILTIATGMEEHNNYGVDFINATRRIKEVCPYAKISGGVSNLSFGFRGVNVIREAIHSVFLYHAVAAGMDMGIVNAGMLEVYDDIPKDLLKLVEDVVLNRHDGATEALLDRSLIEKEKLEAAKKGGVDPNAGIQQEWRTKPIGERLTHALVKGISEFIDQDVEEMRKACARPLDVIEGPLMDGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAKSRLAAAANGEVLEEEDDDSQYAGKVLMATVKGDVHDIGKNIVGVVLGCNNYKIIDMGVMVPCEDILRVAKEEKVDIIGLSGLITPSLDEMVHVAREMNKAGLKIPLMVGGATTSKMHAAVKIAPHYSTADHPVIHVLDASRSVVVVGNLLREDEEREDFVEDLLEEYEELREEYYASLDDIKMISFADAKKKAFQIDFENNPPFQKVNRLGTHLIEHLDLEKLVPFIDWNPFFQTWELRGRYPNRGYPKIFDDESVGEEARKVFDEGQKLLKEIISKKLMHVKGVSGIFPAYR--EGEDVIVCDPSNPDTPVSKFCMLRQQAEKETSDPYMSLADFVAPRRAG--TYDYIGGFAVGVFGVEELAAKFESEHDDFNKIMSQAIGDRLAEAFAEYIHREMRVNDWGYAAEEVLDKEDLLKVKYDGIRPAPGYPSQPDHTEKRVLWELLKAEELAGLTLTDSYMMLPGSAVSALCFAHPESQYFAVGKVSKEQVVEYAERKNQTIEETEKWLAPILGYDR 1286          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: K3WL46_GLOUD (Methionine synthase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WL46_GLOUD)

HSP 1 Score: 1750 bits (4533), Expect = 0.000e+0
Identity = 882/1274 (69.23%), Postives = 1045/1274 (82.03%), Query Frame = 1

Query:   28 PYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREK-QAAAGGKVVKAV---EWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKL---AAQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQ-KEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDR 3825
            P+   E+D +  YL  L +++ +++DG MGTMIQ+YK SEEDYRGE+FKD+  LVKGNND+LSLTQPE+I+ IH+QY+  G++Q+IGTNTFSGTTIA ADY+ME  V ++N+ SARIARE ADEVTA DP +PRFV G+IGPTNRT SISP+VED   RNVTFDEL  AYY Q   L++GGSD+++VETIFDTLNAKAA++AV+ Y E+TG  + PLFISGT+VD SGRTLSGQT EAFY S+RH+KPFC+GLNCALGA QMKPFL+RL+NV ECFV VY+NAGLPNAMGGYDD P  MA     F  ++L+NM+GGCCG+TP HI AIA       PR LP   +P MWLSGLEDL+V+K+RFSFLNVGERCN++GSI FKK IM GDY  AM+IA++QVEDGAMVIDVNVDDGMLDGVAAM++FLKIAVTEP++SKVPFM+DSSKF IVEAGLK VQGK IVNSISLKVG+E F  HA I K+HG A+VVMAFDE+GQAATE +KV +CKRSYDILV+EVGFPPEDIIFDPNILTVATG+EEHNNYGVDFINA + IKEQ PY KISGGVSNLSFGFRGVNVIREA+HSVFLYHAV AGMDMGIVNAGM+EVYDDIPADLL L ED VLNR P ATE LLDRSL+E+EK +AA  G VV      EWRT+P+AERLTH+L+KGI EFID DVEE R+  A+PL+VIEGPLM+GMNVVG+LFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEK  +     A G+  +  DEDS YAGKVLMATVKGDVHDIGKNIV VVLGCNNYK+ D+GVM  CEDIL  A+E  VD+IGLSGLITPSLDEMV+VA+EM+K G+K PLM+GGATTSKMH AVKIAP Y+T EHPVIHVLDASRSV VV +LL   + +E +VEELLE+YEELREE+YASL++   + ++  R+KG Q+DF   PP       G+  ++  +L  +VP+IDWNPFFQTWELRGRYPNRGYPKIF+DE VGAEARK+FD+AQ +L++I+D K +++ GV+ IFPA R  +G+DV V  P+  +A + KFCMLRQQAEKET +PY+SLADF+AP G G   +D++G FAV   G +E A++ EA +DD+NKIM QAL DRL EAFAE +HR++R   WGYA DE L  EDL K++Y GIRPAPGYPSQPDHTEKR +WDLLK  EL G+ L++S  M+P S+VSAL FAHP++QYFAVGK+G+DQV EYA RK   + E E+WL+PIL YDR
Sbjct:   21 PFVRGESD-IFQYLSALMKQRTLIFDGGMGTMIQQYKFSEEDYRGERFKDYDMLVKGNNDLLSLTQPEVIRTIHKQYMEIGEAQMIGTNTFSGTTIAQADYKMEDLVYELNYESARIAREAADEVTALDPLKPRFVAGSIGPTNRTLSISPNVEDPGFRNVTFDELVEAYYTQIEGLVDGGSDILLVETIFDTLNAKAAVYAVDVYQEKTGK-KLPLFISGTIVDMSGRTLSGQTTEAFYVSLRHSKPFCIGLNCALGANQMKPFLQRLSNVAECFVSVYANAGLPNAMGGYDDGPALMAEYCEEFCKDQLINMMGGCCGTTPAHIKAIADVCGKYAPRPLPPPKDPFMWLSGLEDLVVTKERFSFLNVGERCNISGSIRFKKLIMKGDYGTAMDIARQQVEDGAMVIDVNVDDGMLDGVAAMERFLKIAVTEPDVSKVPFMVDSSKFHIVEAGLKCVQGKCIVNSISLKVGKELFCEHARIVKRHGAAVVVMAFDEDGQAATEAEKVRICKRSYDILVNEVGFPPEDIIFDPNILTVATGMEEHNNYGVDFINACKVIKEQNPYCKISGGVSNLSFGFRGVNVIREAIHSVFLYHAVKAGMDMGIVNAGMLEVYDDIPADLLKLCEDVVLNRHPGATEALLDRSLIEKEKVEAAKKGGVVATTNQQEWRTKPIAERLTHSLVKGISEFIDQDVEEMRKVSARPLDVIEGPLMDGMNVVGNLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKNKQRLENTANGIANEVEDEDSQYAGKVLMATVKGDVHDIGKNIVGVVLGCNNYKIIDMGVMVPCEDILRVAREENVDIIGLSGLITPSLDEMVYVAREMSKAGMKIPLMVGGATTSKMHAAVKIAPHYSTAEHPVIHVLDASRSVVVVGNLLRQDEEREEFVEELLEDYEELREEYYASLDDIKVVSYSDARSKGFQIDFVNNPPFQKLNRVGLHVIDDLDLEKLVPYIDWNPFFQTWELRGRYPNRGYPKIFDDENVGAEARKIFDEAQKLLKEIIDKKLMQVRGVSGIFPAYR--EGDDVIVCDPDHPEAPVSKFCMLRQQAEKETSDPYMSLADFIAPAGAG--YQDYIGGFAVGVFGVEEMAAKFEAEHDDFNKIMSQALGDRLAEAFAEYIHREMRVNDWGYAADEVLDKEDLLKVKYDGIRPAPGYPSQPDHTEKRALWDLLKATELIGLELTDSYMMLPGSAVSALCFAHPESQYFAVGKVGKDQVIEYAQRKNFSLEETERWLAPILGYDR 1288          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: H3G8D7_PHYRM (Methionine synthase n=29 Tax=Peronosporaceae TaxID=4777 RepID=H3G8D7_PHYRM)

HSP 1 Score: 1746 bits (4521), Expect = 0.000e+0
Identity = 875/1257 (69.61%), Postives = 1037/1257 (82.50%), Query Frame = 1

Query:   82 QEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAA--GGKVVKA---VEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEK-AAKLA--AQGLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQ-KEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDR 3825
            +++  ++DG MGTMIQ ++ +EED+RGE+F D   LVKGNND+LS+TQP++IK+IH+QY+  G++QLIGTNTFSGTTIA ADY+ME  V ++NF SAR+ARE ADEVT  DP  PRFV G+IGPTNRT SISP+VED   RNVTFDEL  AYY Q   L++GGSDV++VETIFDTLNAKAA+FA+N+Y E TG  + PLFISGT+VD SGRTLSGQT EAFY S+RH+KPFC+GLNCALGA QMKPFL+RLANV ECFV VY+NAGLPNAMGGYDDTP  MA     F+ +RL+NM+GGCCG+TP HI AIA       PR LP L EP MWLSGLED++V+K+RFSFLNVGERCN++GSI FK+ IM GDY  AM++A++QVEDGAMVIDVNVDDGMLDGVAAM++FLKIAVTEP++SKVPFM+DSSKF IVEAGLK VQGK IVNSISLKVG+E F  HA I K HG A+VVMAFDE GQAATE +KV +CKRSYDILV+EVGFPPEDIIFDPNILTVATG+EEHNNYGVDFINA R IKEQ PY KISGGVSNLSFGFRGVNVIREA+HSVFLYHAV AGMDMGIVNA M+EVYDDIP DLL LVED VLNR PD TE LLDRSL+E+EK  AA  GG V  +    EWR++P+ ERLTHAL+KGI EFID DVEE R+   KPL+VIEGPLM+GMNVVG LFG+GKMFLPQVIKSARVMKKAVAYLLP+MEEEK   +LA  A G+  +E DEDS YAGKVL+ATVKGDVHDIGKNIV VVLGCNNYK+ D+GVM  CEDILA A++Y VDVIGLSGLITPSLDEMV+VA+EM+K GLK PL++GGATTSKMH AVKIAP Y+T EHPV+HVLDASRSV VV +LL   + ++ +VEE++EEYEE+RE++YASL++   + F   + K  Q+D+ A+PP       G+  +E   L D+VPFIDWNPFFQTWELRGRYPNRGYPKIF+DEAVGAEARK+FD+AQ +L++I+D K LR+ GV+ IFPA R  +G+DV V  P+  +  + KFCMLRQQAEKET +PY+SLADF+AP+G GG  +D++G FAV   G ++ A++ EA +DD+NKIM QA+ DRL EAFAE +HR++R   WGYA DE L  EDL K++Y GIRPAPGYPSQPDHTEKR +WDLLK +EL  ++L++S  M+P S+VSAL FAHPD+QYFAVGKIG+DQV EYA RKG+ + E E+WL+PIL YDR
Sbjct:    2 KQRTFIFDGGMGTMIQTHRFTEEDFRGERFADSPCLVKGNNDLLSITQPQVIKDIHKQYMQVGRAQLIGTNTFSGTTIAQADYQMEHLVYELNFESARLAREAADEVTLLDPLLPRFVAGSIGPTNRTLSISPNVEDPGFRNVTFDELVDAYYTQVEGLVDGGSDVLLVETIFDTLNAKAAVFAINKYQETTGR-KLPLFISGTIVDMSGRTLSGQTTEAFYVSLRHSKPFCIGLNCALGANQMKPFLQRLANVAECFVSVYANAGLPNAMGGYDDTPAMMAEYCEEFSKDRLINMMGGCCGTTPQHIEAIAIMAAKYEPRPLPELKEPFMWLSGLEDMVVTKERFSFLNVGERCNISGSIRFKRLIMKGDYGTAMDVARQQVEDGAMVIDVNVDDGMLDGVAAMERFLKIAVTEPDVSKVPFMVDSSKFHIVEAGLKCVQGKCIVNSISLKVGKELFMEHARIVKSHGAAVVVMAFDEEGQAATEAEKVRICKRSYDILVNEVGFPPEDIIFDPNILTVATGMEEHNNYGVDFINACRIIKEQNPYCKISGGVSNLSFGFRGVNVIREAIHSVFLYHAVQAGMDMGIVNAAMLEVYDDIPKDLLKLVEDVVLNRNPDGTEALLDRSLIEKEKADAAKKGGVVASSGNKQEWRSKPIGERLTHALVKGISEFIDQDVEEMRKVAEKPLDVIEGPLMDGMNVVGKLFGSGKMFLPQVIKSARVMKKAVAYLLPYMEEEKNQRRLANTANGIANEEEDEDSQYAGKVLIATVKGDVHDIGKNIVGVVLGCNNYKIIDMGVMVPCEDILAAAKKYNVDVIGLSGLITPSLDEMVYVAREMSKAGLKVPLIVGGATTSKMHAAVKIAPHYSTPEHPVMHVLDASRSVVVVGNLLRQDEERDEFVEEVMEEYEEMREDYYASLDDIKLIGFENAKTKSYQIDYTAKPPFQKINRVGLHVIEDLPLEDLVPFIDWNPFFQTWELRGRYPNRGYPKIFDDEAVGAEARKVFDEAQKLLQEIMDKKLLRVRGVSGIFPAYR--EGDDVIVCDPKNPEQEISKFCMLRQQAEKETSDPYISLADFIAPKGVGG--QDYIGGFAVGVFGVEDMAAKFEADHDDFNKIMSQAIGDRLAEAFAEYIHREMRVKDWGYAADEVLNQEDLLKVKYDGIRPAPGYPSQPDHTEKRALWDLLKADELIDLKLTDSYMMLPGSAVSALCFAHPDSQYFAVGKIGKDQVVEYAERKGLTLEETERWLAPILGYDR 1253          

BLAST of mRNA_H-akashiwo_Contig952.8.4 vs. uniprot
Match: A0A067CQR9_SAPPC (Methionine synthase n=4 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067CQR9_SAPPC)

HSP 1 Score: 1740 bits (4507), Expect = 0.000e+0
Identity = 895/1283 (69.76%), Postives = 1033/1283 (80.51%), Query Frame = 1

Query:    4 TKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVD-EVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPYAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAG----GKVVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAVAKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKAAKLAAQ---GLDPQEADEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGQASLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEASRHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 3828
            T   +   PY   +TD  + YL  L   +I+++DG MGTMIQK+K SEE+YRGE+FKD+  LVKGNND+LSLTQ +IIK+IH +Y+  G +QLIGTNTFSGTTIA ADY ME  V +INF SA +ARE  DE+TA +P  PRFV G+IGPTNRT SISP+VED   RNVTFDEL  AYY+Q   LM+GGSD+++VETIFDTLNAKAA+FAV++Y +    T  PLFISGT+VD SGRTLSGQT EAFY S+RH+KPFC+GLNCALGA QMKPFLRRLANV ECFV VY+NAGLPNAMGGYDD P  MA     F  E L+NMIGGCCG+TP HIAAIAA     PPR  P+   P MWLSGLED++V+K+RF+FLNVGERCN++GSI FKK I+ GDY  AME+A+ QVE+GAMV+DVNVDDGMLDGVAAM++FLKIAVTEP++SKVPFMIDSSKF +VEAGLK VQGK IVNSISLKVGE EF RHA I K +G A+VVMAFDE GQAATE +KV +CKRSYDILV   V FPPEDIIFDPNILT+ATG++EHNNYGVDFINA R IK Q PY KISGGVSNLSFGFRGVNVIREAMHSVFLYHAV AGMDMGIVNAGM+++YDDIPADLL +VED VLNR P+ATE+LLDRS++EREK  AA     G VV   EWRT+PV ERLTHAL+KGI ++ID DVEE R   A+PL VIEGPLM+GMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLP+ME EKAAK AA    G+  +  D+DSMYAGKVLMATVKGDVHDIGKNIV VVLGCNNYK+ D GVM  CE+IL  A+E+KVD+IGLSGLITPSLDEMVFVAKEMAK G+ QPLMIGGATTSKMH AVKIAPQYAT +HPVIHVLDASRSV VV +LL   +K  +VEE+LEEYEE+RE++YASL++   + + ++R +GLQ+DFA  PP       G   +E  +L D++PFIDWNPFFQTWELRGRYPNRGYPKIF+DE VGAEA+KLFDDAQ ML++I+  K L + GV  I+ A R   GEDV ++ P+     +  F MLRQQAEKETDEPYLSLADFV+P   G  + DH+G FAV C G +E   ++EA +DDY+KIM QA+ DRLVEAFAE +HRD+R  LWGYA  EEL  EDL K++Y GIRPAPGYPSQPDHTEKRTMW+LL  E L G+ LSE+  MMPASSVSAL FAHP +QYFAVGKI +DQV+ YAARK   V   EKWLSPIL YDRD
Sbjct:    4 THAEILAAPYVRGQTDLFE-YLTALMSTRILIFDGGMGTMIQKHKFSEEEYRGERFKDWPHLVKGNNDLLSLTQRQIIKDIHTEYMEIGGAQLIGTNTFSGTTIAQADYHMEDIVYEINFESAALAREACDELTAKNPLLPRFVAGSIGPTNRTLSISPNVEDPGFRNVTFDELVQAYYEQIEGLMDGGSDILLVETIFDTLNAKAAVFAVDKY-QTDKQTRIPLFISGTIVDMSGRTLSGQTTEAFYVSLRHSKPFCIGLNCALGANQMKPFLRRLANVAECFVSVYANAGLPNAMGGYDDDPVLMAEYCGEFCEEGLLNMIGGCCGTTPKHIAAIAAAAAKHPPRPTPSPKAPFMWLSGLEDMVVTKERFAFLNVGERCNISGSIRFKKLILKGDYSTAMEVARAQVEEGAMVVDVNVDDGMLDGVAAMERFLKIAVTEPDVSKVPFMIDSSKFHVVEAGLKCVQGKCIVNSISLKVGEAEFIRHAKIVKSYGAAVVVMAFDEYGQAATEAEKVRICKRSYDILVGPHVLFPPEDIIFDPNILTIATGMDEHNNYGVDFINACRVIKAQNPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVQAGMDMGIVNAGMLQIYDDIPADLLQIVEDVVLNRNPEATEKLLDRSVIEREKAEAAKKGGTGAVVSQQEWRTKPVNERLTHALVKGISDYIDADVEEMRLLCARPLHVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPYMEAEKAAKQAADKANGIVSEVEDDDSMYAGKVLMATVKGDVHDIGKNIVGVVLGCNNYKIIDAGVMVPCEEILRLAREHKVDIIGLSGLITPSLDEMVFVAKEMAKAGMTQPLMIGGATTSKMHAAVKIAPQYATIDHPVIHVLDASRSVVVVGNLLKEDEKAEFVEEILEEYEEMREDYYASLDDVKMVPYDRIRERGLQIDFATSPPHGHSHFLGNHVVENVDLHDLLPFIDWNPFFQTWELRGRYPNRGYPKIFDDENVGAEAKKLFDDAQKMLKEILKAKSLSVRGVIGIYKAHRV--GEDVVLYDPKQPTKVVATFHMLRQQAEKETDEPYLSLADFVSPAELG--LEDHVGMFAVGCFGVEELGVQYEAKHDDYSKIMAQAIGDRLVEAFAEYIHRDMRVRLWGYAQHEELAQEDLLKVKYDGIRPAPGYPSQPDHTEKRTMWNLLNAEAL-GLHLSENFVMMPASSVSALCFAHPASQYFAVGKIAKDQVEAYAARKKQSVELTEKWLSPILAYDRD 1279