mRNA_H-akashiwo_Contig947.2.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig947.2.1
Unique NamemRNA_H-akashiwo_Contig947.2.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A7S2V6M1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V6M1_9STRA)

HSP 1 Score: 133 bits (334), Expect = 7.080e-34
Identity = 76/216 (35.19%), Postives = 121/216 (56.02%), Query Frame = 3
Query:  114 VGIPTLKNQL-TSVERQRRLKALKFW----GLVVCTFVALAMISSHSMDLSESLTNSISYLTSTPLRQKRFVFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFGALTAHATWEA 746
            +G  +L N L  S+   R L     W    GL V   V L  I++  + +S++LT  I+ +T+    +  +V++GSYT  V  I       G GI  F ++  TG+L+   + +  G+NPS L +   K +L+A NELG  D GK+F+  I  ++G L  ++ ++ PG+NPSSI++ P  +YLA S+FL  GG +++ ++ DGS G LT    W+A
Sbjct:    4 IGEESLYNGLGLSLHELRMLSRTWKWHAVNGLCVFFMVGLLYIATIPLQISDALTEHITSMTAISSSKVAYVYMGSYTTAVTGIKAGEDFHGEGIYSFGVNEETGSLEEIASPLPVGDNPSFLCMHPTKFVLYATNELGLSDTGKVFAMKISPETGALSVMNSVDSPGWNPSSISVHPRGEYLAVSHFLRGGGIALYKLEDDGSIGDLTDFTNWDA 219          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A521P740_9BACT (Lactonase family protein n=1 Tax=Chitinophagaceae bacterium TaxID=1869212 RepID=A0A521P740_9BACT)

HSP 1 Score: 70.9 bits (172), Expect = 2.520e-10
Identity = 51/187 (27.27%), Postives = 89/187 (47.59%), Query Frame = 3
Query:  300 QKRFVFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGS---FGALTAHA------TWEAGSRCHTAAFTPGGAAFVLALDWALDLVLS 833
            Q+ ++FVG+YT              +GI V++ +A  G +Q+  NT +S  NPS L V    + ++A NE G ++ G++ +F  D   G L  L+     G +P  I + P  K+   + + G G  +   ++ DGS   +  L  H         +A +  H+  F+P    ++LA D  +D ++S
Sbjct:   19 QQYYLFVGTYTTG----------KSKGIYVYQFNAQNGEMQWVSNT-DSCTNPSFLAVSPNGNFVYAVNETGRENPGRVSAFAFDKTKGKLQLLNQQASGGDDPCFITVHPSGKWAVVANYSG-GSLAAFPIRTDGSIQPYAQLIQHKGSGPNQARQASAHVHSTFFSPDYQ-YILAPDLGMDKIMS 192          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A5B6TJN7_9BACT (Lactonase family protein n=3 Tax=Rufibacter TaxID=1379908 RepID=A0A5B6TJN7_9BACT)

HSP 1 Score: 68.6 bits (166), Expect = 1.620e-9
Identity = 59/187 (31.55%), Postives = 84/187 (44.92%), Query Frame = 3
Query:  312 VFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGS---QDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFGALT---AHA-----TWEAGSRCHTAAFTPGGAAFVLALDWALDLVLSSR 839
            V+VG+Y K           D   I  F LD  TG L  +   V+ GENPS LT+ +++  L+A NE      Q  G + +F ID  +GGL  L+     G  P  I++    K    + ++G G  S + +QPDG   A T    HA       + G   H     P    F LA+D  +D V+S +
Sbjct:   32 VYVGTYAKP----------DAESIFAFRLDEETGTLT-SAFAVKGGENPSYLTLDKDRKHLYAVNETTEYEGQKSGAVSAFSIDQKTGGLTFLNKQPSLGGAPCYISLDHKNKMAMVANYVG-GNVSAYLLQPDGRLSASTDMEQHAGKGPRQQQDGPHAHCITPDPNNN-FALAVDLGIDQVISYK 205          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A5B9MDT8_9BACT (6-phosphogluconolactonase n=1 Tax=Stieleria maiorica TaxID=2795974 RepID=A0A5B9MDT8_9BACT)

HSP 1 Score: 67.8 bits (164), Expect = 3.220e-9
Identity = 44/141 (31.21%), Postives = 74/141 (52.48%), Query Frame = 3
Query:  315 FVGSYTKEVDSIV---VDCAM-DGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANE---LGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFG 716
            +VG+++  +  ++   VD    +GRGI +F +D TTG L   G T   G +PS L V    + L++ANE   +G+  +G + +F ID  SG L  +  +   G  P+ I++ P  ++L  + + G G  ++  V  DG  G
Sbjct:   43 YVGTFSSPLQDVLPTQVDLPPGNGRGIHLFRVDRTTGQLSAIG-TQRMGTSPSCLAVNEAGTRLYSANETDRVGAAKEGSVSTFSIDRSSGELTPMGTVRSGGAGPTYISLHPSGRFLLVANYFG-GSVAVLPVAADGQLG 181          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A3D2HA39_9BACT (3-carboxymuconate cyclase n=1 Tax=Bacteroidales bacterium TaxID=2030927 RepID=A0A3D2HA39_9BACT)

HSP 1 Score: 66.2 bits (160), Expect = 9.320e-9
Identity = 42/135 (31.11%), Postives = 72/135 (53.33%), Query Frame = 3
Query:  312 VFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFG 716
            + +GSYT    S          GI V+  ++ TG+ +   + V   ENPS LTV R++  ++A NE+    +GK+ SFL++S +G L  L+ +   G +P  +++  + KY+    + G+G  S   ++ DGS G
Sbjct:   28 LLIGSYTSSSKS---------DGIYVYAFNSKTGDAELI-SKVSGEENPSYLTVSRDRKYVYAVNEVR---EGKISSFLLNSTTGELTFLNRVSSGGVSPCYVSVDDNNKYVFVGNY-GNGTLSAIPLKADGSLG 148          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A2T6FC58_9BURK (Hemagglutinin n=1 Tax=Glaciimonas sp. PCH181 TaxID=2133943 RepID=A0A2T6FC58_9BURK)

HSP 1 Score: 61.6 bits (148), Expect = 3.090e-7
Identity = 57/188 (30.32%), Postives = 84/188 (44.68%), Query Frame = 3
Query:  309 FVFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNL---QFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFGALTA----------HATWEAGSRCHTAAFTPGGAAFVLALDWALDLVLS 833
            F +VGS T    S        G GITVF+L+ TTG+L   Q  G+ V    NPS L + R    L+A +     D   + +F +D  +G L  ++     G NP  + I P  +++  S  LG    ++  +  DGS GAL+           H   +  S+ H   F P G  FV+  D  LD + +
Sbjct:    2 FAYVGSRTTRERSA------RGDGITVFQLNQTTGDLSLVQVVGDLV----NPSFLALDRSGDHLYAVHG----DLSDISAFKVDKSTGKLQFINRQSTGGKNPVHLAIDPSGRFIVVSNHLGSS-LAVLPIGADGSLGALSQLVTLTGPLGPHRVEQKLSKPHFNPFDPSGH-FVVVPDKGLDRIFT 173          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A0H4PCM4_9BACT (6-phosphogluconolactonase n=1 Tax=Cyclobacterium amurskyense TaxID=320787 RepID=A0A0H4PCM4_9BACT)

HSP 1 Score: 57.8 bits (138), Expect = 6.430e-6
Identity = 48/174 (27.59%), Postives = 80/174 (45.98%), Query Frame = 3
Query:  288 TPLRQKRFVFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYL-ATSYFLGDGGFSIHSVQPDGSFGALTAHATWEAGS---------RCHTAAFTP 779
            TPL+  RF+ +G+YT   +          +GI + E D  +G +  T +T    +NPS +   + ++L++A  E G ++ GK+ SF +  D   L  L+ +   G +P  I + P EK++ A +Y  GD  F++     +G           E GS           H+A F P
Sbjct:   31 TPLKH-RFL-LGTYTNSPE----------QGIHLMEFDTDSGKIN-TLSTASEPDNPSFVIANQAQNLVYAVEETGGENGGKVSSFSLIDDE--LTKLNTVSAEGQSPCYITLDPSEKFIIAANYSSGD--FTVIPTDENGQLKPAVQVLAHEGGSVVPNRQKQSHVHSAIFHP 187          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A7C3FXB7_9CHLR (Lactonase family protein n=1 Tax=Thermoflexia bacterium TaxID=2420334 RepID=A0A7C3FXB7_9CHLR)

HSP 1 Score: 57.4 bits (137), Expect = 8.050e-6
Identity = 40/144 (27.78%), Postives = 70/144 (48.61%), Query Frame = 3
Query:  294 LRQKRFVFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFGALT 725
            ++Q   ++VG+Y             D  GI+V+E+D TTG L F+ +      NPS L +      L+A NELG Q    + +F +D+++G L  L+  +  G     I+++    +   S +   G  +++ +Q DGS  A +
Sbjct:    2 IKQAVLIYVGAYASG----------DQEGISVYEMDMTTGELTFS-SLAGGVANPSFLALHPSGRFLYAVNELG-QPGSAVSAFAVDAETGALTFLNKQDAHGTAACYISVNDAGTHAYISNYS-SGTAAVYPIQADGSLDAAS 132          
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Match: A0A7G9BSA9_9SPHI (Lactonase family protein n=2 Tax=Olivibacter sp. SDN3 TaxID=2764720 RepID=A0A7G9BSA9_9SPHI)

HSP 1 Score: 54.3 bits (129), Expect = 8.710e-5
Identity = 49/184 (26.63%), Postives = 80/184 (43.48%), Query Frame = 3
Query:  312 VFVGSYTKEVDSIVVDCAMDGRGITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKGKLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGFSIHSVQPDGSFGALTAHATWEAGS----------RCHTAAFTPGGAAFVLALDWALDLVLS 833
            + +G+YT    S         +GI V+  D  +G L +        +NPS LT+ +++  +++ NELG +  G++ +F  DS +  L  L+     G  P  I I  ++K +  S + G G F++   + DG             GS            H+A F+P G  F+L  D   D V S
Sbjct:   18 LLIGTYTDTGKS---------QGIYVYAFDTVSGKLTYRSEVPT--DNPSFLTLSKDRKYVYSVNELG-EGNGRVSAFAYDSSTAKLRFLNQQAALGDAPCHITIDQEDKNVIVSNYNG-GNFAVFGRERDGRL-TEKKQVVGHQGSGPDKSRQEKPHVHSAIFSPNGK-FLLVQDLGTDQVSS 186          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig947.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
A0A7S2V6M1_9STRA7.080e-3435.19Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A521P740_9BACT2.520e-1027.27Lactonase family protein n=1 Tax=Chitinophagaceae ... [more]
A0A5B6TJN7_9BACT1.620e-931.55Lactonase family protein n=3 Tax=Rufibacter TaxID=... [more]
A0A5B9MDT8_9BACT3.220e-931.216-phosphogluconolactonase n=1 Tax=Stieleria maiori... [more]
A0A3D2HA39_9BACT9.320e-931.113-carboxymuconate cyclase n=1 Tax=Bacteroidales ba... [more]
A0A2T6FC58_9BURK3.090e-730.32Hemagglutinin n=1 Tax=Glaciimonas sp. PCH181 TaxID... [more]
A0A0H4PCM4_9BACT6.430e-627.596-phosphogluconolactonase n=1 Tax=Cyclobacterium a... [more]
A0A7C3FXB7_9CHLR8.050e-627.78Lactonase family protein n=1 Tax=Thermoflexia bact... [more]
A0A7G9BSA9_9SPHI8.710e-526.63Lactonase family protein n=2 Tax=Olivibacter sp. S... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig947contigH-akashiwo_Contig947:47244..48670 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog634452.APA01_11250
PFAMsLactonase
Model size839
Max annot lvl204441|Rhodospirillales
Hectar predicted targeting categorysignal anchor
Exons4
Evalue5.34e-14
EggNOG OGsCOG2706@1|root,COG2706@2|Bacteria,1MUKZ@1224|Proteobacteria,2U2PG@28211|Alphaproteobacteria,2JX2M@204441|Rhodospirillales
DescriptionLactonase, 7-bladed beta-propeller
Cds size759
COG categoryG
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388054.0607102-CDS-H-akashiwo_Contig947:47243..473571681388054.0607102-CDS-H-akashiwo_Contig947:47243..47357Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig947 47244..47357 -
1681388054.0745878-CDS-H-akashiwo_Contig947:47526..476351681388054.0745878-CDS-H-akashiwo_Contig947:47526..47635Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig947 47527..47635 -
1681388054.0898304-CDS-H-akashiwo_Contig947:47903..482121681388054.0898304-CDS-H-akashiwo_Contig947:47903..48212Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig947 47904..48212 -
1681388054.1027718-CDS-H-akashiwo_Contig947:48363..485901681388054.1027718-CDS-H-akashiwo_Contig947:48363..48590Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig947 48364..48590 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388054.1141868-UTR-H-akashiwo_Contig947:48590..486701681388054.1141868-UTR-H-akashiwo_Contig947:48590..48670Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig947 48591..48670 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig947.2.1prot_H-akashiwo_Contig947.2.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig947 47244..48590 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig947.2.1

>prot_H-akashiwo_Contig947.2.1 ID=prot_H-akashiwo_Contig947.2.1|Name=mRNA_H-akashiwo_Contig947.2.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=253bp
MNYQEGTFDLAVGIPTLKNQLTSVERQRRLKALKFWGLVVCTFVALAMIS
SHSMDLSESLTNSISYLTSTPLRQKRFVFVGSYTKEVDSIVVDCAMDGRG
ITVFELDATTGNLQFTGNTVESGENPSILTVGREKSLLFAANELGSQDKG
KLFSFLIDSDSGGLVALDDIELPGYNPSSINISPDEKYLATSYFLGDGGF
SIHSVQPDGSFGALTAHATWEAGSRCHTAAFTPGGAAFVLALDWALDLVL
SSR
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mRNA from alignment at H-akashiwo_Contig947:47244..48670-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig947.2.1 ID=mRNA_H-akashiwo_Contig947.2.1|Name=mRNA_H-akashiwo_Contig947.2.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=1427bp|location=Sequence derived from alignment at H-akashiwo_Contig947:47244..48670- (Heterosigma akashiwo CCMP452)
CTCTGAATACCTGAACATATAGATAAGCCTTATAGTTGATACTTCAGATC TCTCTCATCCCGGCGGGTCTGGAGTGTCAGATGAATTACCAGGAGGGGAC ATTCGACCTGGCTGTAGGAATTCCGACGTTGAAAAATCAATTGACTTCTG TAGAAAGGCAACGACGCTTAAAAGCTCTAAAGTTCTGGGGCCTTGTAGTC TGTACTTTTGTTGCCTTAGCAATGATTTCATCCCATTCGATGGACCTTTC AGAGAGCCTGACGAATTCGATATCATATCTTACTTCCACACCCCTTAGAC AGAAGCGGTAAGTCGAGGAGGGCTGGTATGGATGATTCTGATTTCCATTA ATCTTGTCGCCAATTGTGTCTCAGGACTTTTCGCATTTCATGCATTTCGC GACTTTAACTTTCTAATAACAATTTATATAATCAACATACAATGAATACA CCTCACAGGTTTGTGTTTGTTGGAAGTTACACCAAGGAGGTCGATTCCAT TGTTGTCGACTGTGCCATGGATGGGAGGGGTATCACTGTGTTTGAACTAG ACGCAACTACAGGAAACTTGCAATTCACAGGAAACACAGTAGAGTCTGGA GAGAATCCTTCTATTTTGACAGTCGGAAGAGAGAAAAGTCTACTTTTTGC TGCAAATGAGCTTGGGTCTCAGGACAAAGGAAAACTGTTTTCTTTTCTAA TCGACAGTGATTCTGGGGGGCTGGTAGCGCTTGATGATATTGAGCTTCCT GGCTATAATCCAAGCAGGTGGGAGCTACTGTCATCTAGACAGTACAAGAA CAATCTGAAAGTTGCAACTCAGCTTTATGATACTTGTACATACCTGGAAG ATCTACTTTTACTAGTAGTATCAGGATGGATGACGGTACTTGCGTTATCT TTCTTCCATAACACATCATCAGATAAATATTGTCAGCCGAAGAAAGTTGA ATCAAGCCACTTAGTATCTTTTGAATACGATATAATAATTAAACGTTGAA TGCTTCATTGCTGCCCATCTTCCCCACTCTTACAGCATAAACATCTCTCC GGATGAGAAATACCTGGCTACAAGCTATTTTTTGGGCGATGGAGGGTTTT CAATTCATTCCGTTCAGCCTGACGGATCGTTTGGAGCTCTGACGGTGAGT GCGTGCATTTCTTCCCCAACTTGAAACCGCTTTCTTCTTCAGGGAGCAGG GAGGAATCAATGTTGCCATTTTAAAAATCAGAGGGTGTGTATGAGTATAC ACAACAAGATATACCAATGCAATCCCTCGTCTACTTTACCAATGAACTCC ACCACCCACACAGGCACACGCGACTTGGGAAGCCGGCTCGCGCTGCCACA CTGCGGCCTTCACCCCGGGGGGCGCCGCCTTTGTGCTGGCGCTGGATTGG GCGCTGGACCTGGTGCTTTCTTCGCGG
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Coding sequence (CDS) from alignment at H-akashiwo_Contig947:47244..48670-

>mRNA_H-akashiwo_Contig947.2.1 ID=mRNA_H-akashiwo_Contig947.2.1|Name=mRNA_H-akashiwo_Contig947.2.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=759bp|location=Sequence derived from alignment at H-akashiwo_Contig947:47244..48670- (Heterosigma akashiwo CCMP452)
ATGAATTACCAGGAGGGGACATTCGACCTGGCTGTAGGAATTCCGACGTT
GAAAAATCAATTGACTTCTGTAGAAAGGCAACGACGCTTAAAAGCTCTAA
AGTTCTGGGGCCTTGTAGTCTGTACTTTTGTTGCCTTAGCAATGATTTCA
TCCCATTCGATGGACCTTTCAGAGAGCCTGACGAATTCGATATCATATCT
TACTTCCACACCCCTTAGACAGAAGCGGTTTGTGTTTGTTGGAAGTTACA
CCAAGGAGGTCGATTCCATTGTTGTCGACTGTGCCATGGATGGGAGGGGT
ATCACTGTGTTTGAACTAGACGCAACTACAGGAAACTTGCAATTCACAGG
AAACACAGTAGAGTCTGGAGAGAATCCTTCTATTTTGACAGTCGGAAGAG
AGAAAAGTCTACTTTTTGCTGCAAATGAGCTTGGGTCTCAGGACAAAGGA
AAACTGTTTTCTTTTCTAATCGACAGTGATTCTGGGGGGCTGGTAGCGCT
TGATGATATTGAGCTTCCTGGCTATAATCCAAGCAGCATAAACATCTCTC
CGGATGAGAAATACCTGGCTACAAGCTATTTTTTGGGCGATGGAGGGTTT
TCAATTCATTCCGTTCAGCCTGACGGATCGTTTGGAGCTCTGACGGCACA
CGCGACTTGGGAAGCCGGCTCGCGCTGCCACACTGCGGCCTTCACCCCGG
GGGGCGCCGCCTTTGTGCTGGCGCTGGATTGGGCGCTGGACCTGGTGCTT
TCTTCGCGG
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