mRNA_H-akashiwo_Contig1126.7.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1126.7.1
Unique NamemRNA_H-akashiwo_Contig1126.7.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A7S3V009_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3V009_HETAK)

HSP 1 Score: 136 bits (342), Expect = 8.850e-38
Identity = 66/75 (88.00%), Postives = 68/75 (90.67%), Query Frame = 2 
Query:    2 MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDMEN--TETTSTSNGSSGGDERHEVNWSAHFNAPHPNVTQRMYFV 220
            MYTACTRSVSAVPPAFYAHLMAFRGAFYIHD E   TETTSTSNGSSGGDE+ EVNW  HFNAPHPNVT+RMYFV
Sbjct:  115 MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDTEGSITETTSTSNGSSGGDEQQEVNWGQHFNAPHPNVTKRMYFV 189
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A7S3UXA3_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UXA3_HETAK)

HSP 1 Score: 136 bits (342), Expect = 2.590e-34
Identity = 66/75 (88.00%), Postives = 68/75 (90.67%), Query Frame = 2 
Query:    2 MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDMEN--TETTSTSNGSSGGDERHEVNWSAHFNAPHPNVTQRMYFV 220
            MYTACTRSVSAVPPAFYAHLMAFRGAFYIHD E   TETTSTSNGSSGGDE+ EVNW  HFNAPHPNVT+RMYFV
Sbjct:  799 MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDTEGSITETTSTSNGSSGGDEQQEVNWGQHFNAPHPNVTKRMYFV 873
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: C0PNQ0_MAIZE (Piwi domain-containing protein n=1 Tax=Zea mays TaxID=4577 RepID=C0PNQ0_MAIZE)

HSP 1 Score: 58.9 bits (141), Expect = 1.950e-7
Identity = 27/47 (57.45%), Postives = 35/47 (74.47%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIH-DMENTETTSTSNGSSGG 142
            Y  CTRSVS VPPA+YAHL AFR  FY+  +M   +T+ +SNG++GG
Sbjct:  239 YARCTRSVSVVPPAYYAHLAAFRARFYMEPEMSENQTSKSSNGTNGG 285
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A1D6P2M1_MAIZE (Argonaute101 n=10 Tax=PACMAD clade TaxID=147370 RepID=A0A1D6P2M1_MAIZE)

HSP 1 Score: 58.9 bits (141), Expect = 2.760e-7
Identity = 27/47 (57.45%), Postives = 35/47 (74.47%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIH-DMENTETTSTSNGSSGG 142
            Y  CTRSVS VPPA+YAHL AFR  FY+  +M   +T+ +SNG++GG
Sbjct:  940 YARCTRSVSVVPPAYYAHLAAFRARFYMEPEMSENQTSKSSNGTNGG 986
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A1D6LSF6_MAIZE (Argonaute10b n=3 Tax=Zea mays TaxID=4577 RepID=A0A1D6LSF6_MAIZE)

HSP 1 Score: 58.5 bits (140), Expect = 3.730e-7
Identity = 28/46 (60.87%), Postives = 35/46 (76.09%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIH-DMENTETTSTSNGSSG 139
            Y  CTRSVS VPPA+YAHL AFR  FY+  +M + +T+ TSNG+SG
Sbjct:  876 YARCTRSVSVVPPAYYAHLAAFRARFYMEPEMLDNQTSKTSNGTSG 921
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: F0W310_9STRA (Argonaute4 (AGO4) putative n=2 Tax=Albugo TaxID=65356 RepID=F0W310_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 3.820e-7
Identity = 30/73 (41.10%), Postives = 42/73 (57.53%), Query Frame = 2 
Query:    2 MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGGDERHEVNWSAHFNAPHPNVTQRMYFV 220
            M+  CTRSVS VPP +YAHL AFR  F++++M +  +T       G D     N+   F+  H  V+ RMYF+
Sbjct: 1156 MFARCTRSVSVVPPVYYAHLAAFRARFFLNEMSDGASTI------GSDNSKASNYD--FSHVHEQVSNRMYFI 1220
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A830C5V8_9LAMI (Protein argonaute mel1 n=1 Tax=Phtheirospermum japonicum TaxID=374723 RepID=A0A830C5V8_9LAMI)

HSP 1 Score: 58.2 bits (139), Expect = 5.100e-7
Identity = 26/46 (56.52%), Postives = 35/46 (76.09%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGG 142
            Y  CTRSVS VPPA+YAHL AFR  +Y+  +E +++ STS G++GG
Sbjct:  913 YARCTRSVSIVPPAYYAHLAAFRARYYMEGIEMSDSGSTSAGAAGG 958
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: UPI001C36130B (Uncharacterized protein n=1 Tax=Carya illinoinensis TaxID=32201 RepID=UPI001C36130B)

HSP 1 Score: 57.0 bits (136), Expect = 6.050e-7
Identity = 27/46 (58.70%), Postives = 37/46 (80.43%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGG 142
            Y  CTRSVS VPPA+YAHL+AFR  FY+ D E++E+ S ++G++GG
Sbjct:  148 YARCTRSVSIVPPAYYAHLVAFRARFYM-DPESSESGSMASGTAGG 192
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BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A2K3PG38_TRIPR (Argonaute protein n=1 Tax=Trifolium pratense TaxID=57577 RepID=A0A2K3PG38_TRIPR)

HSP 1 Score: 57.4 bits (137), Expect = 8.310e-7
Identity = 30/72 (41.67%), Postives = 43/72 (59.72%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGGDERHEVNWSAHFNAPHPNVTQRMYFV 220
            Y  CTRSVS VPPA+YAHL+AFR  +Y+ D  +T  + ++NG +    R+  N  A   +   +V   M+FV
Sbjct:  369 YARCTRSVSIVPPAYYAHLLAFRARYYLSDAADTSDSGSANGGT----RNATNVVAALPSIIESVKDNMFFV 436
[Close]
BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Match: A0A6P5XMM0_DURZI (protein argonaute 5-like isoform X1 n=2 Tax=Durio zibethinus TaxID=66656 RepID=A0A6P5XMM0_DURZI)

HSP 1 Score: 57.4 bits (137), Expect = 9.440e-7
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = 2 
Query:    5 YTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGGDER 151
            Y  CTRSVS VPPA+YAHL AFR  +Y+ D EN+++ ST   S+GG  R
Sbjct:  905 YARCTRSVSIVPPAYYAHLAAFRARYYVED-ENSDSGSTGGESTGGGRR 952
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1126.7.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3V009_HETAK8.850e-3888.00Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S3UXA3_HETAK2.590e-3488.00Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
C0PNQ0_MAIZE1.950e-757.45Piwi domain-containing protein n=1 Tax=Zea mays Ta... [more]
A0A1D6P2M1_MAIZE2.760e-757.45Argonaute101 n=10 Tax=PACMAD clade TaxID=147370 Re... [more]
A0A1D6LSF6_MAIZE3.730e-760.87Argonaute10b n=3 Tax=Zea mays TaxID=4577 RepID=A0A... [more]
F0W310_9STRA3.820e-741.10Argonaute4 (AGO4) putative n=2 Tax=Albugo TaxID=65... [more]
A0A830C5V8_9LAMI5.100e-756.52Protein argonaute mel1 n=1 Tax=Phtheirospermum jap... [more]
UPI001C36130B6.050e-758.70Uncharacterized protein n=1 Tax=Carya illinoinensi... [more]
A0A2K3PG38_TRIPR8.310e-741.67Argonaute protein n=1 Tax=Trifolium pratense TaxID... [more]
A0A6P5XMM0_DURZI9.440e-757.14protein argonaute 5-like isoform X1 n=2 Tax=Durio ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1126contigH-akashiwo_Contig1126:203270..204013 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FQK3
PFAMsArgoL1,ArgoL2,ArgoMid,ArgoN,PAZ,Piwi
Model size446
Max annot lvl2759|Eukaryota
KEGG koko:K11593
Hectar predicted targeting categoryother localisation
Exons2
Evalue1.12e-10
EggNOG OGsKOG1041@1|root,KOG1041@2759|Eukaryota
Descriptiongene silencing by RNA
Cds size222
COG categoryK
BRITEko00000,ko03019,ko03036
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387090.7721605-UTR-H-akashiwo_Contig1126:203269..2034921681387090.7721605-UTR-H-akashiwo_Contig1126:203269..203492Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1126 203270..203492 -
1681387090.815331-UTR-H-akashiwo_Contig1126:204012..2040131681387090.815331-UTR-H-akashiwo_Contig1126:204012..204013Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1126 204013..204013 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387090.7855313-CDS-H-akashiwo_Contig1126:203492..2036181681387090.7855313-CDS-H-akashiwo_Contig1126:203492..203618Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1126 203493..203618 -
1681387090.8022702-CDS-H-akashiwo_Contig1126:203916..2040121681387090.8022702-CDS-H-akashiwo_Contig1126:203916..204012Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1126 203917..204012 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1126.7.1prot_H-akashiwo_Contig1126.7.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1126 203493..204012 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1126.7.1

>prot_H-akashiwo_Contig1126.7.1 ID=prot_H-akashiwo_Contig1126.7.1|Name=mRNA_H-akashiwo_Contig1126.7.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=74bp
MYTACTRSVSAVPPAFYAHLMAFRGAFYIHDMENTETTSTSNGSSGGDER
HEVNWSAHFNAPHPNVTQRMYFV*
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mRNA from alignment at H-akashiwo_Contig1126:203270..204013-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1126.7.1 ID=mRNA_H-akashiwo_Contig1126.7.1|Name=mRNA_H-akashiwo_Contig1126.7.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=744bp|location=Sequence derived from alignment at H-akashiwo_Contig1126:203270..204013- (Heterosigma akashiwo CCMP452)
CATGTATACTGCCTGTACAAGGTCAGTATCAGCTGTGCCACCAGCTTTCT
ATGCGCATCTGATGGCATTCCGGGGAGCCTTCTACATTCACGACATGGTA
TGTTTTCTTTAGCAGGCTACATTTGTATTATAGCTATATGTACGACGCCC
TTATACAACGAATTTCATGTTCCAAAATTCTTAAAGCAATGTCCTCAGCT
GACAGGCTTGCATCAAGACAATTCTACTTATTATTGCAATTTGTTGGGGT
AACATGTTGTAGTAATATCAATTCTTCATCATGTGGCGCACCATTCTTTC
ATGCTCTCTCTCTCTCTCTTATGCACTTGATTCTTCCCTTTCTGTCTCTG
CAAACCCAAACGAACAACATCACCAACCACATTCCCTGGTTGCAGGAGAA
TACAGAGACAACCAGCACCAGCAACGGCTCCAGCGGTGGTGATGAGCGGC
ATGAAGTCAACTGGAGCGCACACTTCAATGCTCCACATCCAAATGTGACT
CAAAGAATGTACTTTGTGTGAAAATTTAGCTGCTATACTGACAAAGTGTA
GTTAGGGAAAATATAGGAAAAGGAAGAAGAAAAGAGATGGTCATGAGAGA
AAATGAGACAGGTTCGTGGAAAGGCTACTACTATGTACAAGCGGCAGATT
ATGATCACGAGTGGGTGATGACAAACAAGAAGAGGTTTCATAGTAGTACA
CTGCTGCTGTGTACTGTAGTAGTGAAGATGCATCATTGACAAAA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1126:203270..204013-

>mRNA_H-akashiwo_Contig1126.7.1 ID=mRNA_H-akashiwo_Contig1126.7.1|Name=mRNA_H-akashiwo_Contig1126.7.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=222bp|location=Sequence derived from alignment at H-akashiwo_Contig1126:203270..204013- (Heterosigma akashiwo CCMP452)
ATGTATACTGCCTGTACAAGGTCAGTATCAGCTGTGCCACCAGCTTTCTA
TGCGCATCTGATGGCATTCCGGGGAGCCTTCTACATTCACGACATGGAGA
ATACAGAGACAACCAGCACCAGCAACGGCTCCAGCGGTGGTGATGAGCGG
CATGAAGTCAACTGGAGCGCACACTTCAATGCTCCACATCCAAATGTGAC
TCAAAGAATGTACTTTGTGTGA
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