mRNA_H-akashiwo_Contig1.105.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1.105.1
Unique NamemRNA_H-akashiwo_Contig1.105.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
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BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A7S3XY21_HETAK (DNA gyrase subunit B n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XY21_HETAK)

HSP 1 Score: 209 bits (532), Expect = 9.320e-61
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 2 
Query:  245 MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT
Sbjct:    1 MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 101
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A383AXX9_9ZZZZ (HATPase_c domain-containing protein (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A383AXX9_9ZZZZ)

HSP 1 Score: 158 bits (400), Expect = 4.900e-47
Identity = 73/99 (73.74%), Postives = 87/99 (87.88%), Query Frame = 2 
Query:  251 SSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            S+ V N+ YGA+QI VLEGLEPVRKRPGMYIGSTGP+GLHHL++EVVDNS+DEALAGHCD I I++  DGS  + DNGRGIP ++HP+TG+SALETVLT
Sbjct:    7 SNKVSNE-YGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLIYEVVDNSVDEALAGHCDHIEIVLKADGSALISDNGRGIPTDIHPRTGKSALETVLT 104
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A352JFX6_9CYAN (DNA topoisomerase IV subunit B (Fragment) n=1 Tax=Pseudanabaena sp. TaxID=1153 RepID=A0A352JFX6_9CYAN)

HSP 1 Score: 157 bits (398), Expect = 1.580e-46
Identity = 74/93 (79.57%), Postives = 82/93 (88.17%), Query Frame = 2 
Query:  269 KAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            + Y A+QI VLEGLE VRKRPGMYIGSTGPKGLHHLVFEVVDNS+DEALAGHCD IL+ +  DGS+SV DNGRGIP + HPKTG+SALETVLT
Sbjct:    7 ETYNANQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVFEVVDNSVDEALAGHCDHILVELMSDGSVSVSDNGRGIPTDTHPKTGKSALETVLT 99
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: T2RDJ1_PAES9 (Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase family protein n=1 Tax=Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) TaxID=1292036 RepID=T2RDJ1_PAES9)

HSP 1 Score: 156 bits (394), Expect = 6.370e-46
Identity = 72/91 (79.12%), Postives = 82/91 (90.11%), Query Frame = 2 
Query:  275 YGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            YGA QI VLEGLEPVRKRPGMYIGSTGP+GLHHLV+E+VDNSIDEALAG+C +I + I+ DGS+ V+DNGRGIP EVHPKTG+S LETVLT
Sbjct:    5 YGASQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEIVDNSIDEALAGYCSDIYVSINEDGSIFVRDNGRGIPVEVHPKTGKSTLETVLT 95
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A8J8AS25_9FIRM (HATPase_c domain-containing protein (Fragment) n=1 Tax=Proteocatella sp. TaxID=2819256 RepID=A0A8J8AS25_9FIRM)

HSP 1 Score: 156 bits (394), Expect = 8.710e-46
Identity = 72/93 (77.42%), Postives = 81/93 (87.10%), Query Frame = 2 
Query:  269 KAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            K YGADQI VLEGLEPVRKRPGMYIGSTGPKGLHHLV+EVVDN+IDEALAGHC  I + I  D S++V D+GRGIP E+HPKTG++ LETVLT
Sbjct:   22 KDYGADQIQVLEGLEPVRKRPGMYIGSTGPKGLHHLVYEVVDNAIDEALAGHCQNIYVAIEKDNSITVTDDGRGIPVEIHPKTGKTTLETVLT 114
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A356NCY3_9SYNE (DNA topoisomerase IV subunit B (Fragment) n=1 Tax=Synechococcus sp. UBA8638 TaxID=2055762 RepID=A0A356NCY3_9SYNE)

HSP 1 Score: 156 bits (394), Expect = 9.560e-46
Identity = 71/91 (78.02%), Postives = 82/91 (90.11%), Query Frame = 2 
Query:  275 YGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            YGA QI VLEGLEPVRKRPGMYIGSTGP+GLHHLV+EVVDN++DEALAGHC EI + +H DGS  V+DNGRGIP ++HP+TG+SALETVLT
Sbjct:   11 YGAAQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNAVDEALAGHCTEIHVDLHGDGSAEVRDNGRGIPTDIHPRTGKSALETVLT 101
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A6N2EIC5_9CYAN (DNA gyrase subunit B n=12 Tax=Cyanobacteria TaxID=1117 RepID=A0A6N2EIC5_9CYAN)

HSP 1 Score: 168 bits (426), Expect = 9.870e-46
Identity = 78/101 (77.23%), Postives = 88/101 (87.13%), Query Frame = 2 
Query:  245 MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            M  +T     YGA+QI VLEGLEPVRKRPGMYIGSTGP+GLHHLV+EVVDNS+DEALAGHCD+IL++IH DGS  V DNGRGIP +VHP+TGRSALETVLT
Sbjct:    1 MSEATKVQNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDQILVVIHADGSCGVTDNGRGIPTDVHPRTGRSALETVLT 101
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A354B1I0_9CYAN (DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1 Tax=Synechococcales bacterium UBA10510 TaxID=2055795 RepID=A0A354B1I0_9CYAN)

HSP 1 Score: 162 bits (410), Expect = 1.100e-45
Identity = 75/101 (74.26%), Postives = 87/101 (86.14%), Query Frame = 2 
Query:  245 MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            M  +T    AYGA+QI VLEGLEPVRKRPGMYIG+TGP+GLHHLV+EVVDNS+DEALAGHCD IL+ ++ DGS  V DNGRGIP ++HP+TGRSALETVLT
Sbjct:    1 MSEATKVEAAYGAEQIQVLEGLEPVRKRPGMYIGTTGPRGLHHLVYEVVDNSVDEALAGHCDRILVALNDDGSCLVTDNGRGIPTDIHPRTGRSALETVLT 101
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: K9P9X0_CYAGP (DNA gyrase subunit B n=7 Tax=Cyanobacteria TaxID=1117 RepID=K9P9X0_CYAGP)

HSP 1 Score: 167 bits (423), Expect = 2.550e-45
Identity = 76/92 (82.61%), Postives = 86/92 (93.48%), Query Frame = 2 
Query:  272 AYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            AYGA+QI VLEGLEPVRKRPGMYIG+TGP+GLHHLV+EVVDNS+DEALAGHCDEIL+ +H DGS SV DNGRGIP ++HP+TGRSALETVLT
Sbjct:   10 AYGAEQIQVLEGLEPVRKRPGMYIGTTGPRGLHHLVYEVVDNSVDEALAGHCDEILVTLHGDGSASVTDNGRGIPTDIHPRTGRSALETVLT 101
[Close]
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Match: A0A4P7ZTJ9_9SYNE (DNA gyrase subunit B n=5 Tax=Synechococcales TaxID=1890424 RepID=A0A4P7ZTJ9_9SYNE)

HSP 1 Score: 166 bits (420), Expect = 6.880e-45
Identity = 76/101 (75.25%), Postives = 90/101 (89.11%), Query Frame = 2 
Query:  245 MMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVVDNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETVLT 547
            M  +T    AYGA+QI VLEGLEPVRKRPGMYIGSTGP+GLHHLV+EVVDNS+DEALAGHCD+IL++++ DGS +V DNGRGIP +VHP+TG+SALETVLT
Sbjct:    1 MSEATKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDQILVVLNEDGSCAVTDNGRGIPTDVHPRTGKSALETVLT 101
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1.105.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3XY21_HETAK9.320e-61100.00DNA gyrase subunit B n=1 Tax=Heterosigma akashiwo ... [more]
A0A383AXX9_9ZZZZ4.900e-4773.74HATPase_c domain-containing protein (Fragment) n=1... [more]
A0A352JFX6_9CYAN1.580e-4679.57DNA topoisomerase IV subunit B (Fragment) n=1 Tax=... [more]
T2RDJ1_PAES96.370e-4679.12Histidine kinase-, DNA gyrase B-, and HSP90-like A... [more]
A0A8J8AS25_9FIRM8.710e-4677.42HATPase_c domain-containing protein (Fragment) n=1... [more]
A0A356NCY3_9SYNE9.560e-4678.02DNA topoisomerase IV subunit B (Fragment) n=1 Tax=... [more]
A0A6N2EIC5_9CYAN9.870e-4677.23DNA gyrase subunit B n=12 Tax=Cyanobacteria TaxID=... [more]
A0A354B1I0_9CYAN1.100e-4574.26DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1... [more]
K9P9X0_CYAGP2.550e-4582.61DNA gyrase subunit B n=7 Tax=Cyanobacteria TaxID=1... [more]
A0A4P7ZTJ9_9SYNE6.880e-4575.25DNA gyrase subunit B n=5 Tax=Synechococcales TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1contigH-akashiwo_Contig1:4490003..4492391 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog292564.Cyagr_2242
Preferred namegyrB
PFAMsDNA_gyraseB,DNA_gyraseB_C,HATPase_c,Intein_splicing,Toprim
Model size548
Max annot lvl1117|Cyanobacteria
KEGG koko:K02470
Hectar predicted targeting categoryother localisation
Exons5
Evalue1.37e-47
EggNOG OGsCOG0187@1|root,COG0187@2|Bacteria,1G139@1117|Cyanobacteria,22SG4@167375|Cyanobium
EC5.99.1.3
DescriptionA type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Cds size306
COG categoryL
BRITEko00000,ko01000,ko03032,ko03400
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1.105.1prot_H-akashiwo_Contig1.105.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1 4490003..4492075 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387035.7704723-CDS-H-akashiwo_Contig1:4490002..44900741681387035.7704723-CDS-H-akashiwo_Contig1:4490002..4490074Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1 4490003..4490074 -
1681387035.782672-CDS-H-akashiwo_Contig1:4491267..44913351681387035.782672-CDS-H-akashiwo_Contig1:4491267..4491335Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1 4491268..4491335 -
1681387035.7916179-CDS-H-akashiwo_Contig1:4491822..44918971681387035.7916179-CDS-H-akashiwo_Contig1:4491822..4491897Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1 4491823..4491897 -
1681387035.805961-CDS-H-akashiwo_Contig1:4491984..44920751681387035.805961-CDS-H-akashiwo_Contig1:4491984..4492075Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1 4491985..4492075 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387035.822173-UTR-H-akashiwo_Contig1:4492075..44920841681387035.822173-UTR-H-akashiwo_Contig1:4492075..4492084Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1 4492076..4492084 -
1681387035.8382895-UTR-H-akashiwo_Contig1:4492159..44923911681387035.8382895-UTR-H-akashiwo_Contig1:4492159..4492391Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1 4492160..4492391 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1.105.1

>prot_H-akashiwo_Contig1.105.1 ID=prot_H-akashiwo_Contig1.105.1|Name=mRNA_H-akashiwo_Contig1.105.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=102bp
MMMSSTVGNKAYGADQITVLEGLEPVRKRPGMYIGSTGPKGLHHLVFEVV
DNSIDEALAGHCDEILIIIHPDGSLSVQDNGRGIPCEVHPKTGRSALETV
LT
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mRNA from alignment at H-akashiwo_Contig1:4490003..4492391-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1.105.1 ID=mRNA_H-akashiwo_Contig1.105.1|Name=mRNA_H-akashiwo_Contig1.105.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=2389bp|location=Sequence derived from alignment at H-akashiwo_Contig1:4490003..4492391- (Heterosigma akashiwo CCMP452)
AACAAAAAAGCGAGGAGGAGGGCGACACGATTGGACATTCTACTTTTTGT
GGGATCGGATAATCAGCACAATGAAGTGCTATATCCCTCTCGCTGTTTTG
GGCTTTGCCTGCATGCGCTGCTGGCAGGAGGTCTCAGCGTTTACCCCTGG
GAAATCACATATGATCAGAGCTGGGAAAGGCTCTCCCTATCATGTACGAC
GTTGATGCGTTCGTTTCTTCCTCTCAAATATTGCATGAAACATGATAATG
GCACTAGGTTTTCATCCTCACTACTTTTTTTAATGTGCACCATGTCTTTG
ATTCTAGAAGAGGATAATGATGATGTCATCGACAGTTGGCAACAAGGCAT
ATGGGGCAGATCAAATTACAGTTTTGGAAGGACTGGAGCCAGTCAGGAAA
CGGCCAGGCAAGTATGAAGTCGTGCAACTTGGGTCGGAAAAGAAAATTAC
TTGTCCCAATGAACTGCTTATTAACTTATGAATCTCAACTCCAGGAATGT
ACATTGGTTCAACTGGGCCGAAGGGGCTCCATCACTTGGTGTTTGAAGTG
GTTGACAATTCAATTGATGGTAAGCTCATCCTTGTCTTCATTTTTTTCTC
TGCAGTACAGTGATGAGTTCGGCTTGGATTATTGTTGAGATGGCATTACC
ATCAAATGTATTATATATAATGAAGCATTATATATATATAATGTGCATGT
ATAGCAAGTATTACTCGGATGTCTTTTCCTTTATATATATGGTATAGGGA
CCTGATAATAGCGATGCTCATTTTTGGAATCCACCCACCATCAGCAAGTA
GTACCCTGTGCATGAAAGAATGCATGCAGTATTTTTTAGATAATGTTCAA
ACATAAAAATGAAATGGAAAATTAAGAGCCCATCACTTATGCACTGCTAC
TGCCACCCATTTTTAGCTCTGGCTTTGCTACGGATTTCTAATTCGCATAG
AAGCACAACTCCCTTTCATTTTTTACAACAGCCGTATGCCTGTCCGAGCT
CCCACTTTGCTCTTCATAATCTCTTTCCTCTTTTCATGACCACTATCTCT
CCACAGAGGCACTTGCGGGGCACTGTGATGAAATATTGATTATTATCCAC
CCAGATGGAAGCCTGTCTGTCCAGGTATGATTTTTTGTTTCCTCTACATT
TGTATTATGCAATGCGCTCCTCTCATATCCTGGGCCAAAACCACTGTGTT
ATTTTACATGCGCCATGGCATCTGAGCAACTAGTCGAACTTGCAGCTCTC
TGGACATTTCTTCCTCAACAAGACTCTCTCTCTTAGTCTCCCCATCTCGG
CAAGTTGAATCGTCTTCCTCCTTTTCCTTCTTTATTTTTCTATTGTGCTA
TGTTCAGTAATTGTGATAGATTAATTTTGTGATACATATATATATTACAA
GATAGATGATGATCTAGGATGATTGCATTTTGAGATGCTCAAAATAGGAT
AATTTTGATGGCAATGAAATTTAGTATTTTGATGGAAGCGCAAAGTTCTA
TCATCCTGGATCATCAAAACTGGTGATGATGAAAAACAACCAAGCTATGC
TAAAAACAACTAGGTTGGGTGATGATGATGAATTTGGGTGATGTAGGGGG
AGATGACTGGTCTCTACTACCCATAGGCCAGTCATCTGACACAAACAGCC
AATGAAGGAAACACAACTAACACAGCCAATGAAAACAGCAAACAGAAGAG
GTCGGCTCGTGGCAACATCGATCAGTGGGAGTGGCAAAAGTCCAAAGTGA
CACATCGAGTCAGCGGGATCAGCTAAGCGAGATGGTCTTGATATGTTTGG
GGCCTAACTCCTCAGATGGGTAATGCAGTCGAGCTCAGCTGATAAACTCT
AATGATGTGATGAGTCCATAACAGGGTTATTGACGAATCCAGAAAAGGTG
AATGTGGTGATGCCAAGAGGTCTTGGTCATCTATGCGCAAAGACACAAGC
CGGTGACTGAGGAAAACGATAGGATTGCGCATTGTCACCGGGAGTGGTCT
TGGCAAGCTGAAAACGGGAGAGCAACGATGAAACACGACGAGGCACAAAC
AGAGGGGCGAAAGCTCTCTGATTTAATGTATTATCCAGCCATATATTAGC
TACGAGCATTGAAATGGGGGGGGTTAGACTCCATTGTCGTATAAGTCATT
TTAAGGCTATAATAATAGTTCTTTATTGAGACATTGAGCAGGAGAAGTGA
GTCGCTTGGAAAGAAGATTATTTTGACGAGGTCATCAATCAAGCGTTCCT
CGCCGCCCCCCCCCCACCACGCACCCCCCGCCCGCCCGCCCGCCGCCGCC
CGCCGCCGCCGCCGCAGGACAACGGGCGGGGCATCCCCTGCGAGGTGCAC
CCGAAGACCGGCCGCAGCGCCCTGGAGACGGTGCTGACC
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1:4490003..4492391-

>mRNA_H-akashiwo_Contig1.105.1 ID=mRNA_H-akashiwo_Contig1.105.1|Name=mRNA_H-akashiwo_Contig1.105.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=306bp|location=Sequence derived from alignment at H-akashiwo_Contig1:4490003..4492391- (Heterosigma akashiwo CCMP452)
ATGATGATGTCATCGACAGTTGGCAACAAGGCATATGGGGCAGATCAAAT
TACAGTTTTGGAAGGACTGGAGCCAGTCAGGAAACGGCCAGGAATGTACA
TTGGTTCAACTGGGCCGAAGGGGCTCCATCACTTGGTGTTTGAAGTGGTT
GACAATTCAATTGATGAGGCACTTGCGGGGCACTGTGATGAAATATTGAT
TATTATCCACCCAGATGGAAGCCTGTCTGTCCAGGACAACGGGCGGGGCA
TCCCCTGCGAGGTGCACCCGAAGACCGGCCGCAGCGCCCTGGAGACGGTG
CTGACC
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