Homology
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
F4NZ18_BATDJ (TGc domain-containing protein n=2 Tax=Batrachochytrium dendrobatidis TaxID=109871 RepID=F4NZ18_BATDJ)
HSP 1 Score: 140 bits (353), Expect = 6.010e-38
Identity = 69/123 (56.10%), Postives = 83/123 (67.48%), Query Frame = 1
Query: 1 YRAGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPN----LDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
Y A VVELYQC C + TRFPRYN +KLLETR GRCGE+ANV AL ++GFDTR+V D+TDHVW EVY Q+RW+H DS D P+ YE +GW KKLTYVIA+ ++V
Sbjct: 168 YGAKVVELYQCESCHKYTRFPRYNDPAKLLETRHGRCGEWANVFALCCKTMGFDTRYVVDFTDHVWTEVYDTTQNRWVHCDSCEGEGAYDTPLMYE---------TGWEKKLTYVIAIGVYDV 281
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
UPI00106B3C69 (peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase-like isoform X1 n=2 Tax=Dendronephthya gigantea TaxID=151771 RepID=UPI00106B3C69)
HSP 1 Score: 141 bits (355), Expect = 2.420e-37
Identity = 66/119 (55.46%), Postives = 82/119 (68.91%), Query Frame = 1
Query: 7 AGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPN--LDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
AG VELYQCPKCK++ RFPRYN KLLETR GRCGE+AN + +LGF TR+V DWTDHVW EV+S+ Q +WLH D D P+ YE +GWNKKLTY+IA + +++
Sbjct: 111 AGRVELYQCPKCKKIMRFPRYNHPQKLLETRIGRCGEWANAFTMCCIALGFKTRYVLDWTDHVWTEVFSDAQQKWLHCDPCEGICDRPLLYE---------AGWNKKLTYIIAFAKNQI 220
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A7SSF4_NEMVE (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (Fragment) n=4 Tax=Nematostella vectensis TaxID=45351 RepID=A7SSF4_NEMVE)
HSP 1 Score: 142 bits (357), Expect = 4.230e-37
Identity = 69/118 (58.47%), Postives = 79/118 (66.95%), Query Frame = 1
Query: 10 GVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPNL--DAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
GVVE Y+CP C + RFPRYN +KLLETR GRCGE+AN +F LGF+TRHV DWTDHVW EVYSE Q RWLH DS D P+ YE +GW KKL+Y+IA S EV
Sbjct: 251 GVVEEYKCPTCSQQIRFPRYNHPAKLLETRCGRCGEWANCFTMFCRVLGFETRHVIDWTDHVWTEVYSESQSRWLHCDSCECACDKPLVYE---------AGWGKKLSYIIAFSKEEV 359
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
UPI00077A6D00 (peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase-like n=1 Tax=Acropora digitifera TaxID=70779 RepID=UPI00077A6D00)
HSP 1 Score: 139 bits (349), Expect = 6.280e-37
Identity = 66/119 (55.46%), Postives = 77/119 (64.71%), Query Frame = 1
Query: 1 YRAGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPNLDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
+ AG VE Y+CPKC R RFPRYN KLL+TR GRCGE+AN AL ++GF+ RHVTDWTDHVWVEV+S+ Q RW+H D D YE W KKLTY+IA S EV
Sbjct: 28 WEAGRVEAYKCPKCGRKERFPRYNHPGKLLKTRCGRCGEFANCKALILRAMGFEVRHVTDWTDHVWVEVFSDSQQRWIHCDGGKCDENFLYE---------RWWKKKLTYIIAFSKDEV 137
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A0A6P8HB87_ACTTE (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase n=1 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8HB87_ACTTE)
HSP 1 Score: 141 bits (356), Expect = 9.880e-37
Identity = 68/119 (57.14%), Postives = 80/119 (67.23%), Query Frame = 1
Query: 7 AGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPN--LDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
AG VE Y+CPKC+ RFPRYN +KLLETRRGRCGE+AN L +LG++ RHV DWTDHVW E++SE Q RWLH DS D P+ YE +GW KKL+YVIA S EV
Sbjct: 382 AGTVENYRCPKCQHDIRFPRYNHPTKLLETRRGRCGEWANCFTLLCRTLGYEARHVVDWTDHVWTEIFSESQQRWLHCDSCEGICDKPLLYE---------AGWGKKLSYVIAFSRDEV 491
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A0A814MIM0_9BILA (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase n=1 Tax=Brachionus calyciflorus TaxID=104777 RepID=A0A814MIM0_9BILA)
HSP 1 Score: 140 bits (353), Expect = 1.480e-36
Identity = 68/120 (56.67%), Postives = 81/120 (67.50%), Query Frame = 1
Query: 1 YRAGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHAD--SPNLDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHE 354
++AG VE+Y+C C +TRFPRYN SKLLETR GRCGE+AN L SL FD R++ DWTDHVW EVYSE Q RWLHAD LD P+ YE GWNK+L+Y+IA S +E
Sbjct: 238 WQAGNVEVYRCTTCNELTRFPRYNHPSKLLETRGGRCGEWANCFCLILRSLDFDARYILDWTDHVWCEVYSESQQRWLHADPCENTLDKPLLYE---------HGWNKQLSYIIAFSRYE 348
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A0A7S4P662_GUITH (Hypothetical protein (Fragment) n=3 Tax=Guillardia theta TaxID=55529 RepID=A0A7S4P662_GUITH)
HSP 1 Score: 141 bits (355), Expect = 1.480e-36
Identity = 66/119 (55.46%), Postives = 79/119 (66.39%), Query Frame = 1
Query: 7 AGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHAD--SPNLDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
AGVVELY C C + TRFPRYN KL+ET+RGRCGE+AN L ++GF+ RHV DWTDHVW EV+SEDQ RW+H D + D+P+ Y GW KKL+YVIA S EV
Sbjct: 399 AGVVELYHCNDCNKTTRFPRYNHPGKLMETKRGRCGEWANAFTLCCIAMGFEARHVVDWTDHVWTEVFSEDQQRWIHCDPCEDSWDSPLLYS---------EGWGKKLSYVIAFSKDEV 508
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A0A7D9DEH3_PARCT (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase n=1 Tax=Paramuricea clavata TaxID=317549 RepID=A0A7D9DEH3_PARCT)
HSP 1 Score: 139 bits (349), Expect = 1.680e-36
Identity = 66/118 (55.93%), Postives = 78/118 (66.10%), Query Frame = 1
Query: 10 GVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADSPN--LDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
G VELYQCPKCK++ RFPRYN KLLETR GRCGE+AN + +LGF TR+V DWTDHVW EV+SE Q RWLH D D P+ YE +GW KKLTY IA + ++
Sbjct: 111 GRVELYQCPKCKKIMRFPRYNHPQKLLETRHGRCGEWANAFTMCCVALGFKTRYVLDWTDHVWTEVFSEAQQRWLHCDPCEDVCDRPLLYE---------AGWGKKLTYCIAFAKDQI 219
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
A0A3M7SYA2_BRAPC (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase n=1 Tax=Brachionus plicatilis TaxID=10195 RepID=A0A3M7SYA2_BRAPC)
HSP 1 Score: 139 bits (351), Expect = 2.750e-36
Identity = 68/118 (57.63%), Postives = 80/118 (67.80%), Query Frame = 1
Query: 7 AGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHAD--SPNLDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHE 354
AG VE+Y+C C+ +TRFPRYN SKLL+TR GRCGE+AN L S+ FD R+V DWTDHVW EVYSE Q RWLHAD LD P+ YE GWNK+LTY+IA S +E
Sbjct: 242 AGNVEVYKCSNCQTLTRFPRYNHPSKLLDTRGGRCGEWANCFCLVLRSMDFDARYVLDWTDHVWCEVYSESQKRWLHADPCENTLDKPLVYE---------HGWNKQLTYIIAFSRYE 350
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Match:
UPI00077A7C7E (peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase-like n=1 Tax=Acropora digitifera TaxID=70779 RepID=UPI00077A7C7E)
HSP 1 Score: 136 bits (342), Expect = 7.920e-36
Identity = 64/119 (53.78%), Postives = 81/119 (68.07%), Query Frame = 1
Query: 7 AGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTSLGFDTRHVTDWTDHVWVEVYSEDQHRWLHADS--PNLDAPMTYEVRSVLESPQSGWNKKLTYVIAVSPHEV 357
AG VE Y+C C + TRFPRYN +KLLETR+GRCGE+AN L ++GF++RHV DWTDHVW E+YSE Q RWLH D+ + D P+ YE +GW KKL+Y+IA S +V
Sbjct: 165 AGNVESYKCQNCGKKTRFPRYNHPAKLLETRQGRCGEWANCFTLICRAMGFESRHVLDWTDHVWTEIYSEAQQRWLHCDACENSCDKPLIYE---------AGWGKKLSYIIAFSHEQV 274
The following BLAST results are available for this feature:
BLAST of mRNA_H-fluviatilis_contig100139.86.1 vs. uniprot
Analysis Date: 2022-09-19 (
Diamond blastx: OGS1.0 vs UniRef90 )
Total hits: 25
0 1 Expect = 6.01e-38 / Id = 56.10 Expect = 2.42e-37 / Id = 55.46 Expect = 4.23e-37 / Id = 58.47 Expect = 6.28e-37 / Id = 55.46 Expect = 9.88e-37 / Id = 57.14 Expect = 1.48e-36 / Id = 56.67 Expect = 1.48e-36 / Id = 55.46 Expect = 1.68e-36 / Id = 55.93 Expect = 2.75e-36 / Id = 57.63 Expect = 7.92e-36 / Id = 53.78 Sequence F4NZ18_BATDJ UPI00106B3C69 A7SSF4_NEMVE UPI00077A6D00 A0A6P8HB87_ACTTE A0A814MIM0_9BILA A0A7S4P662_GUITH A0A7D9DEH3_PARCT A0A3M7SYA2_BRAPC UPI00077A7C7E Pages
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Alignments
The following features are aligned
Analyses
This mRNA is derived from or has results from the following analyses
Properties
Property Name Value
Taxonomic scope Metazoa Seed ortholog score 140.6 Seed ortholog evalue 4.8e-31 Seed eggNOG ortholog 45351.EDO33371 Preferred name NGLY1 KEGG ko ko:K01456 KEGG Pathway ko04141,map04141 GOs GO:0000224,GO:0001558,GO:0003674,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0006355,GO:0006357,GO:0006457,GO:0006464,GO:0006508,GO:0006511,GO:0006515,GO:0006516,GO:0006517,GO:0006807,GO:0006950,GO:0007154,GO:0007267,GO:0007275,GO:0007399,GO:0007494,GO:0007495,GO:0008150,GO:0008152,GO:0008361,GO:0009056,GO:0009057,GO:0009100,GO:0009889,GO:0009966,GO:0009967,GO:0009987,GO:0010033,GO:0010243,GO:0010468,GO:0010498,GO:0010556,GO:0010646,GO:0010647,GO:0010721,GO:0010769,GO:0010771,GO:0010975,GO:0010977,GO:0015035,GO:0015036,GO:0016043,GO:0016491,GO:0016651,GO:0016667,GO:0016668,GO:0016787,GO:0016810,GO:0016811,GO:0019219,GO:0019222,GO:0019538,GO:0019725,GO:0019941,GO:0022008,GO:0022603,GO:0022604,GO:0023051,GO:0023052,GO:0023056,GO:0030154,GO:0030163,GO:0030246,GO:0030308,GO:0030433,GO:0030510,GO:0030513,GO:0031323,GO:0031326,GO:0031344,GO:0031345,GO:0032501,GO:0032502,GO:0032535,GO:0033554,GO:0034976,GO:0035295,GO:0036211,GO:0036503,GO:0040008,GO:0042221,GO:0042592,GO:0043161,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0045454,GO:0045595,GO:0045596,GO:0045664,GO:0045665,GO:0045926,GO:0047134,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048565,GO:0048583,GO:0048584,GO:0048638,GO:0048640,GO:0048670,GO:0048671,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050768,GO:0050770,GO:0050771,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051093,GO:0051128,GO:0051129,GO:0051171,GO:0051239,GO:0051241,GO:0051252,GO:0051603,GO:0051716,GO:0051960,GO:0051961,GO:0055114,GO:0055123,GO:0060255,GO:0060284,GO:0061387,GO:0065007,GO:0065008,GO:0071704,GO:0071840,GO:0080090,GO:0090066,GO:0090092,GO:0090100,GO:0090287,GO:0120035,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1901698,GO:1903506,GO:2000026,GO:2000112,GO:2001141 EggNOG free text desc. Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins EggNOG OGs 38EIG@33154,3BAEH@33208,KOG0909@1,KOG0909@2759 EC 3.5.1.52 COG Functional cat. O Best tax level Metazoa Best eggNOG OG NA|NA|NA BRITE ko00000,ko00001,ko01000 Hectar predicted targeting category other localisation Exons 5 Model size 357 Cds size 357 Stop 0 Start 0
Relationships
The following polypeptide feature(s) derives from this mRNA:
The following CDS feature(s) are a part of this mRNA:
Feature Name Unique Name Species Type Position
1622928997.8645718-CDS-H-fluviatilis_contig100139:71..110 1622928997.8645718-CDS-H-fluviatilis_contig100139:71..110 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 72..110 + 1691135144.6986246-CDS-H-fluviatilis_contig100139:71..110 1691135144.6986246-CDS-H-fluviatilis_contig100139:71..110 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 72..110 + 1622928997.8768404-CDS-H-fluviatilis_contig100139:166..220 1622928997.8768404-CDS-H-fluviatilis_contig100139:166..220 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 167..220 + 1691135144.7139337-CDS-H-fluviatilis_contig100139:166..220 1691135144.7139337-CDS-H-fluviatilis_contig100139:166..220 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 167..220 + 1622928997.8942158-CDS-H-fluviatilis_contig100139:269..293 1622928997.8942158-CDS-H-fluviatilis_contig100139:269..293 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 270..293 + 1691135144.7231314-CDS-H-fluviatilis_contig100139:269..293 1691135144.7231314-CDS-H-fluviatilis_contig100139:269..293 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 270..293 + 1622928997.912513-CDS-H-fluviatilis_contig100139:325..412 1622928997.912513-CDS-H-fluviatilis_contig100139:325..412 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 326..412 + 1691135144.7343104-CDS-H-fluviatilis_contig100139:325..412 1691135144.7343104-CDS-H-fluviatilis_contig100139:325..412 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 326..412 + 1622928997.9336293-CDS-H-fluviatilis_contig100139:443..596 1622928997.9336293-CDS-H-fluviatilis_contig100139:443..596 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 444..596 + 1691135144.745991-CDS-H-fluviatilis_contig100139:443..596 1691135144.745991-CDS-H-fluviatilis_contig100139:443..596 Heribaudiella fluviatilis SAG_13_90 CDS H-fluviatilis_contig100139 444..596 +
Sequences
The following sequences are available for this feature:
protein sequence of mRNA_H-fluviatilis_contig100139.86.1
>prot_H-fluviatilis_contig100139.86.1 ID=prot_H-fluviatilis_contig100139.86.1|Name=mRNA_H-fluviatilis_contig100139.86.1|organism=Heribaudiella fluviatilis SAG_13_90|type=polypeptide|length=119bp
YRAGVVELYQCPKCKRVTRFPRYNVASKLLETRRGRCGEYANVCALFTTS back to top mRNA from alignment at H-fluviatilis_contig100139:72..596+
Legend: polypeptide CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-fluviatilis_contig100139.86.1 ID=mRNA_H-fluviatilis_contig100139.86.1|Name=mRNA_H-fluviatilis_contig100139.86.1|organism=Heribaudiella fluviatilis SAG_13_90|type=mRNA|length=525bp|location=Sequence derived from alignment at H-fluviatilis_contig100139:72..596+ (Heribaudiella fluviatilis SAG_13_90)TACAGAGCTGGTGTTGTGGAACTCTACCAGTGCCCCAAG GTTGTCGTGTG
GCATAGTCAAGTGCAATCACGTTGTGCGTGTGCATCCAGGCGGAGTGCAA
GCGCGTCACACGCTTTCCGCGTTACAATGTGGCATCCAAACTGCTGGAG G
TGCGTGCCGACTGTCATCGTAGTTTTCAGGACTTGTGTTTGTGCGCAGAC
TCGACGCGGGAGGTGTGGAGAG GTAACCGCCTATGAAATCAAAGAATGGG
ATAGTACGCGAATGTGTGTGCATTGTTCACCACCAGTTTGGGCTTTGACA
CCAGGCACGTGACAGATTGGACCGACCATGTCTGGGTCGAG GTGAGTCCG
GACATACACTATGCTCCGTCAGGTGTATTCTGAAGATCAGCATCGTTGGT
TGCATGCCGATTCTCCGAATTTGGATGCGCCCATGACATATGAGGTTCGA
TCTGTGCTTGAGTCTCCCCAGTCCGGTTGGAACAAGAAGCTGACTTATGT
CATTGCTGTATCACCTCATGAGGTT back to top Coding sequence (CDS) from alignment at H-fluviatilis_contig100139:72..596+
>mRNA_H-fluviatilis_contig100139.86.1 ID=mRNA_H-fluviatilis_contig100139.86.1|Name=mRNA_H-fluviatilis_contig100139.86.1|organism=Heribaudiella fluviatilis SAG_13_90|type=CDS|length=714bp|location=Sequence derived from alignment at H-fluviatilis_contig100139:72..596+ (Heribaudiella fluviatilis SAG_13_90) back to top