mRNA_H-canaliculatus_M_contigs832.5.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_M_contigs832.5.1
Unique NamemRNA_H-canaliculatus_M_contigs832.5.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)
Homology
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Farnesyltransferase, (CAAX box, alpha n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LJX2_ECTSI)

HSP 1 Score: 191 bits (486), Expect = 1.300e-57
Identity = 92/109 (84.40%), Postives = 99/109 (90.83%), Query Frame = 1 
Query:    1 MDDDDLRVPLEGFEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            MD DDL+VPL GFEDVTPIPQ+D  +PVV IAY+HNY VA DYLRAFQ SGETSER+LAITRLVLEYNASHYTAWWFRRRCLFALGMDL +EL+V EDIAGDNPKNYQV
Sbjct:    1 MDVDDLQVPLRGFEDVTPIPQDDSADPVVSIAYRHNYTVATDYLRAFQASGETSERSLAITRLVLEYNASHYTAWWFRRRCLFALGMDLANELEVAEDIAGDNPKNYQV 109
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Farnesyltransferase, (CAAX box, alpha n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YWW6_9STRA)

HSP 1 Score: 125 bits (315), Expect = 2.240e-32
Identity = 60/103 (58.25%), Postives = 75/103 (72.82%), Query Frame = 1 
Query:   19 RVPLEGFEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            + PL G+EDVTPIPQ +  NPV  IAY  +Y+ A DY RA Q SGE SERAL +T  +++ N++HYTAW +RRRCLFAL  DL +EL  V D+A  NPKNYQ+
Sbjct:    7 QQPLPGWEDVTPIPQAECENPVARIAYASDYETAMDYFRAAQASGELSERALELTADIIDMNSAHYTAWQYRRRCLFALNKDLAEELSYVRDVALSNPKNYQI 109
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Hypothetical (protein n=1 Tax=Lotharella oceanica TaxID=641309 RepID=A0A7S2X914_9EUKA)

HSP 1 Score: 113 bits (283), Expect = 1.470e-27
Identity = 59/108 (54.63%), Postives = 73/108 (67.59%), Query Frame = 1 
Query:   13 DLRVPLEG---FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            DL  PL     FED+TPI Q+DGPNPVV IAY  ++    DY RA    GE SERALA+TR V++ N ++YT W FRR CLFALG +L +EL  V+ IA  + KNYQ+
Sbjct:   12 DLTFPLSSSALFEDITPIRQDDGPNPVVSIAYSQHFVDVMDYFRAILKKGEHSERALALTREVIKVNPANYTGWLFRRECLFALGANLKEELKFVDQIAAVSSKNYQL 119
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YNC8_AURAN)

HSP 1 Score: 105 bits (261), Expect = 2.810e-25
Identity = 56/105 (53.33%), Postives = 68/105 (64.76%), Query Frame = 1 
Query:   28 LEGFEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFAL--GMD---LVDELDVVEDIAGDNPKNYQV 327
            +  +EDV PI Q+DGPNPVVPIAY   Y     + RAF  SGE S R L +T  +LE+NA+HYT W  RR+CLFAL  G D   L DE+    D+A  NPKNYQ+
Sbjct:    2 MAAYEDVVPIAQDDGPNPVVPIAYPAEYARLMGFFRAFLASGERSPRVLDLTADLLEHNAAHYTVWHVRRQCLFALADGGDATVLGDEMAYSSDVASGNPKNYQI 106
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein n=5 Tax=Rhizopus TaxID=4842 RepID=A0A367J486_RHIAZ)

HSP 1 Score: 106 bits (264), Expect = 5.980e-25
Identity = 52/94 (55.32%), Postives = 67/94 (71.28%), Query Frame = 1 
Query:   37 FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKN 318
            ++DVTPIPQ+DGPNP+VPIAY   YK A DY RA   + E SER L +T+ +LE N +HYT W +R+  LFAL  DL +ELD +++IA D  KN
Sbjct:    9 WKDVTPIPQDDGPNPLVPIAYSTEYKDAMDYFRAVIRANEKSERVLQLTQDILERNPAHYTVWNYRQDVLFALNKDLNEELDYIDEIAADQAKN 102
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Protein (farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha n=1 Tax=Gossypium arboreum TaxID=29729 RepID=A0A0B0NT77_GOSAR)

HSP 1 Score: 103 bits (257), Expect = 6.380e-25
Identity = 51/96 (53.12%), Postives = 63/96 (65.62%), Query Frame = 1 
Query:   37 FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQ 324
            + DVTP+PQ+DGPNPVVPIAYK  ++   DY RA   + E S RAL +TR  +  N  +YT W FRR  L AL  DL +ELD ++ IA  N KNYQ
Sbjct:   18 WRDVTPLPQDDGPNPVVPIAYKEEFRETMDYFRAIYKADERSPRALRLTRRAIHLNPGNYTVWHFRRLVLEALNADLDEELDFLQRIANSNSKNYQ 113
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein n=1 Tax=Mucor plumbeus TaxID=97098 RepID=A0A8H7VCP4_9FUNG)

HSP 1 Score: 106 bits (264), Expect = 6.420e-25
Identity = 49/97 (50.52%), Postives = 68/97 (70.10%), Query Frame = 1 
Query:   37 FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            ++D+TPIPQ+DGPNP+VPIAY   Y  A DY RA   + E S+R L + + V++ N +HYT W +R++ LF+L  DL  ELD +++IA D  KNYQV
Sbjct:   12 WKDITPIPQDDGPNPLVPIAYSREYADAMDYFRAVTRANEKSQRVLTLIQDVIDMNPAHYTVWNYRQQVLFSLNADLNQELDYIDEIAADQAKNYQV 108
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein n=1 Tax=Glycine max TaxID=3847 RepID=C6TD99_SOYBN)

HSP 1 Score: 103 bits (256), Expect = 1.490e-24
Identity = 53/106 (50.00%), Postives = 69/106 (65.09%), Query Frame = 1 
Query:   19 RVPLEG---FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            RVPL     + DVTP+PQ DGPNPVVPI Y   +    DY RA  ++ E S RALA+T   +++N+ +YT W FRR  L +L +DL DEL+ VE +A  N KNYQ+
Sbjct:   14 RVPLRERVEWSDVTPVPQNDGPNPVVPIQYTEEFSEVMDYFRAVYLTDERSPRALALTAEAVQFNSGNYTVWHFRRLLLESLKVDLNDELEFVERMAAGNSKNYQM 119
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein n=1 Tax=Daucus carota subsp. sativus TaxID=79200 RepID=A0A162B876_DAUCS)

HSP 1 Score: 105 bits (261), Expect = 2.500e-24
Identity = 53/106 (50.00%), Postives = 69/106 (65.09%), Query Frame = 1 
Query:   19 RVPL---EGFEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            RVPL   E + DV P+ Q DGPNPVVPIAY   +    DY RA  ++ E S R+L +T L +  NA +YT W FRR  L AL ++L +ELD V+ I+ +NPKNYQ+
Sbjct:    6 RVPLSEQEEWSDVIPVTQNDGPNPVVPIAYSEEFSETMDYFRAVYLADERSPRSLKLTALAIRLNAGNYTVWQFRRLILEALNVNLNEELDFVDSISSNNPKNYQI 111
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BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Match: Uncharacterized (protein n=2 Tax=Fagus sylvatica TaxID=28930 RepID=A0A2N9GTT7_FAGSY)

HSP 1 Score: 104 bits (260), Expect = 3.710e-24
Identity = 54/106 (50.94%), Postives = 70/106 (66.04%), Query Frame = 1 
Query:   19 RVPLEG---FEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVSGETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIAGDNPKNYQV 327
            R+PL     + DVTP+PQ+DGPNPVVPIAYK ++    DY RA   + E S RAL++T   + +NA++YT W FRR  L AL  DL  EL  +E+IA  N KNYQ+
Sbjct:   13 RIPLSQRPEWSDVTPLPQDDGPNPVVPIAYKPDFVETMDYFRAVYQADERSSRALSLTAEAIHFNAANYTVWHFRRLILEALDADLQVELGFIENIASGNSKNYQI 118
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_M_contigs832.5.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Farnesyltransferase,1.300e-5784.40CAAX box, alpha n=2 Tax=Ectocarpus TaxID=2879 RepI... [more]
Farnesyltransferase,2.240e-3258.25CAAX box, alpha n=1 Tax=Tribonema minus TaxID=3033... [more]
Hypothetical1.470e-2754.63protein n=1 Tax=Lotharella oceanica TaxID=641309 R... [more]
Uncharacterized2.810e-2553.33protein (Fragment) n=1 Tax=Aureococcus anophageffe... [more]
Uncharacterized5.980e-2555.32protein n=5 Tax=Rhizopus TaxID=4842 RepID=A0A367J4... [more]
Protein6.380e-2553.13farnesyltransferase/geranylgeranyltransferase type... [more]
Uncharacterized6.420e-2550.52protein n=1 Tax=Mucor plumbeus TaxID=97098 RepID=A... [more]
Uncharacterized1.490e-2450.00protein n=1 Tax=Glycine max TaxID=3847 RepID=C6TD9... [more]
Uncharacterized2.500e-2450.00protein n=1 Tax=Daucus carota subsp. sativus TaxID... [more]
Uncharacterized3.710e-2450.94protein n=2 Tax=Fagus sylvatica TaxID=28930 RepID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_M_contigs832contigH-canaliculatus_M_contigs832:8358..10436 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male2022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D8LJX2
Preferred nameRAM2
PFAMsPPTA
Model size449
Max annot lvl2759|Eukaryota
KEGG rclassRC00069,RC03004
KEGG koko:K05955
KEGG ReactionR09844
KEGG Pathwayko00900,ko01130,map00900,map01130
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0003824,GO:0004311,GO:0004659,GO:0004660,GO:0004661,GO:0004662,GO:0004663,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005953,GO:0005965,GO:0006464,GO:0006807,GO:0006950,GO:0007323,GO:0008104,GO:0008150,GO:0008152,GO:0008318,GO:0008360,GO:0009414,GO:0009415,GO:0009628,GO:0009787,GO:0009788,GO:0009966,GO:0009968,GO:0009987,GO:0010035,GO:0010467,GO:0010646,GO:0010648,GO:0016740,GO:0016765,GO:0018342,GO:0018343,GO:0018344,GO:0019538,GO:0022603,GO:0022604,GO:0023051,GO:0023057,GO:0032991,GO:0033036,GO:0034613,GO:0036211,GO:0042221,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0048509,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051128,GO:0051179,GO:0051604,GO:0051641,GO:0065007,GO:0065008,GO:0070727,GO:0071704,GO:0072657,GO:0072659,GO:0097354,GO:0140096,GO:1901419,GO:1901420,GO:1901564,GO:1901700,GO:1902494,GO:1905957,GO:1905958,GO:1990234,GO:1990778
Exons4
Evalue2.14e-58
EggNOG OGsCOG5536@1|root,KOG0530@2759|Eukaryota
Ec32 ortholog descriptionProtein prenyltransferase, alpha subunit
Ec32 orthologEc-01_010010.1
EC2.5.1.58,2.5.1.59
DescriptionCAAX-protein geranylgeranyltransferase activity
Cds size447
COG categoryO
BRITEko00000,ko00001,ko01000,ko01006
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854147.4552114-CDS-H-canaliculatus_M_contigs832:8357..84661696854147.4552114-CDS-H-canaliculatus_M_contigs832:8357..8466Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs832 8358..8466 +
1696854147.4704213-CDS-H-canaliculatus_M_contigs832:9285..94151696854147.4704213-CDS-H-canaliculatus_M_contigs832:9285..9415Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs832 9286..9415 +
1696854147.4828286-CDS-H-canaliculatus_M_contigs832:9858..99481696854147.4828286-CDS-H-canaliculatus_M_contigs832:9858..9948Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs832 9859..9948 +
1696854147.4932501-CDS-H-canaliculatus_M_contigs832:10318..104361696854147.4932501-CDS-H-canaliculatus_M_contigs832:10318..10436Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs832 10319..10436 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_M_contigs832.5.1prot_H-canaliculatus_M_contigs832.5.1Hapterophycus canaliculatus Oshoro7m malepolypeptideH-canaliculatus_M_contigs832 8358..10436 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_M_contigs832.5.1

>prot_H-canaliculatus_M_contigs832.5.1 ID=prot_H-canaliculatus_M_contigs832.5.1|Name=mRNA_H-canaliculatus_M_contigs832.5.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=149bp
MDDDDLRVPLEGFEDVTPIPQEDGPNPVVPIAYKHNYKVAGDYLRAFQVS
GETSERALAITRLVLEYNASHYTAWWFRRRCLFALGMDLVDELDVVEDIA
GDNPKNYQVWYHRRALAERTGSAGDELAYVDRVVEEDPKNYHAWSHRQW
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mRNA from alignment at H-canaliculatus_M_contigs832:8358..10436+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_M_contigs832.5.1 ID=mRNA_H-canaliculatus_M_contigs832.5.1|Name=mRNA_H-canaliculatus_M_contigs832.5.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=2079bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs832:8358..10436+ (Hapterophycus canaliculatus Oshoro7m male)
ATGGACGATGACGACCTGCGAGTGCCGCTAGAAGGCTTTGAAGATGTCAC
GCCCATCCCTCAAGAGGATGGTCCCAATCCCGTCGTGCCTATCGCCTACA
AACATAACTGTGAGTTGACCCAGAATGCAACCTGTCGGGAATATTCAAAT
TGCGATCGCGCGAAAAAATGTCACCTGCATCAACTTGAAGACTCACCCTC
CTTTGTTTGTATAAACGAGCGAGATGAATGCTTGCATGGAGAAGCACCGG
GACGTGCCACGCGCACCTTCCATCGAGTTGTTCATCGTGACCAATGCTTC
TCCATGTATTTCCGACAGGTGCTATCGCGGTCGTTTCACAACTTCTTCTA
GTCGTTTTTGTGTCTTGTCTAGTTCTGAAGGTATACCACAGAAGAACGAC
TTTCAGGAATTCACGCTGTGTCCCCCCACATGTAGGTGCTGTGCCATGCC
AGTTCTGTTTACGACCATCATTTGCGATCACCACACCCATGAAAGGTTTA
CGACGGCGACCGGGGTAACGATCCAAGTCACACTTGCACGTGCTACCCCT
CGAGGAGCACCGCCCAAAAAAATTCGTCGCTACACAGACCTCCTTGGGCG
GCTAGACCTACGATCCCCTCCTGTGGGGTAAGAAATCAGTAGTGTGCAAG
GGAGGAAACTACCACCGCCCAAGACGGGACTTAACTCAAGGTCGAGAACT
GCTTCTACCTCTCGGATGGTCGGCTTATTTTTGCGGTTTGCTCCACGCTA
TATTGACGCAGGGGTTAGCCTACGCACGGACAACCACAGCTTTGACCGCT
AGCTCAAGCAGTCGATTGAATGCACCCGGAAACTATGGCAACCATGAGAA
GCCGGAGGGGACATCAGGGTTCTCTCCGGCCTCCCTCGCTGACGCCTAGA
GTTTAAACTTTGCTACCACGATCATCAGATAAGGTTGCCGGGGACTACCT
GAGGGCGTTCCAGGTCTCTGGAGAGACGAGCGAGAGAGCACTGGCTATAA
CGAGACTGGTCCTAGAGTACAACGCCTCACACTACACCGCGTGGTGGTTC
CGTCGAAGGTGGGTGCGCGCATTTCAAAGCACGAGCTCTGCGTTGCGCAA
AAAGGTTGGGAGATGAACGGGGAGGGGCAAACGCGAACACTCACGGACGG
GCCGAGTGTCGTACAAGAACAGCAGTACCAGGAAAAGGGGTTCAAGTTGG
GCTCCAGGGTACTTGTTGGCCGCAAACGTTGGTGTGTTGAGCACCAGACC
GCAGCAGCGTCTCCCGCAGTCTGTAGATTACCCGTAGGTCAAAAAGGTGG
ATCAACGTCACTTGCAACCGTTATGCCGCTGGTGGTATGTCCTCTCTTGC
TACATCGGCACCGGAGAGTCTCATGCCGTCTACATAACTATTCACGAGGG
CCTTCGCCAAGAGGTTATCGTCCTACCTTGATCAGCAGACAGCCTGTTTA
ATTTAAATTGATGAATCTACGTCGGTGATGACGACGACGCCGGCGAGGCA
GGTGCCTCTTCGCGCTCGGGATGGACCTGGTTGACGAACTGGACGTCGTT
GAAGACATCGCCGGCGACAACCCAAAAAACTACCAGGTTTGGTGAGTTCC
GCACGCGCAAGCACGGACGAGAATGTCAATTGCCGCGGCGTGCCCGACAG
CGATAATGCTGGCGTAGCTTTGGCGTCTGTAGCAGCAGCTCTTGGCCTGG
CACGTTGTCATCAAGCTTTGGCCACCGTTCTTATCGCCGAACTCTGGTCG
TGTTGCTCATGGTGTCGGTGCTATAGAAAGAGAGGCGTTCTCTGTGGCGG
CTCCGAAGCAGGCGAGGGCCACCGCGCGCTCGCCCGCGGCTGACGCTAGT
TGGGCTTTGAAGCCCACTGTCCGGCATGATCGGAACGTTTCCACGAGTTG
TATGCCTTGCCTCTCCTGACCTTGGTCTGTTGCTGGTGCTTGGATCGATT
CCATGTTCTAGGTATCACCGGCGCGCTCTAGCGGAGCGCACGGGGAGCGC
GGGAGACGAGCTGGCTTACGTCGATCGAGTCGTGGAGGAGGACCCTAAGA
ACTACCACGCCTGGTCCCACCGGCAGTGG
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Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs832:8358..10436+

>mRNA_H-canaliculatus_M_contigs832.5.1 ID=mRNA_H-canaliculatus_M_contigs832.5.1|Name=mRNA_H-canaliculatus_M_contigs832.5.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=447bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs832:8358..10436+ (Hapterophycus canaliculatus Oshoro7m male)
ATGGACGATGACGACCTGCGAGTGCCGCTAGAAGGCTTTGAAGATGTCAC
GCCCATCCCTCAAGAGGATGGTCCCAATCCCGTCGTGCCTATCGCCTACA
AACATAACTATAAGGTTGCCGGGGACTACCTGAGGGCGTTCCAGGTCTCT
GGAGAGACGAGCGAGAGAGCACTGGCTATAACGAGACTGGTCCTAGAGTA
CAACGCCTCACACTACACCGCGTGGTGGTTCCGTCGAAGGTGCCTCTTCG
CGCTCGGGATGGACCTGGTTGACGAACTGGACGTCGTTGAAGACATCGCC
GGCGACAACCCAAAAAACTACCAGGTTTGGTATCACCGGCGCGCTCTAGC
GGAGCGCACGGGGAGCGCGGGAGACGAGCTGGCTTACGTCGATCGAGTCG
TGGAGGAGGACCCTAAGAACTACCACGCCTGGTCCCACCGGCAGTGG
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