mRNA_H-canaliculatus_F_contig13847.3053.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig13847.3053.1
Unique NamemRNA_H-canaliculatus_F_contig13847.3053.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A6H5KXN7_9PHAE (Transmembrane 9 superfamily member n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KXN7_9PHAE)

HSP 1 Score: 197 bits (501), Expect = 3.720e-58
Identity = 96/103 (93.20%), Postives = 100/103 (97.09%), Query Frame = 1 
Query:    1 GPDAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQSSQ 309
            GPDAG+GEDSGWKQVHGDVFRAPKHLM+FSALFGTGCQL VLVL+VILFAIAGPLHGDVYEERGEMV+TFIVCYALTS VSGY SGSYYRQFFTTPRAEQSSQ
Sbjct:  273 GPDAGVGEDSGWKQVHGDVFRAPKHLMLFSALFGTGCQLAVLVLLVILFAIAGPLHGDVYEERGEMVTTFIVCYALTSLVSGYMSGSYYRQFFTTPRAEQSSQ 375
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: D8LEY0_ECTSI (Transmembrane 9 superfamily member n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEY0_ECTSI)

HSP 1 Score: 157 bits (398), Expect = 6.130e-45
Identity = 74/82 (90.24%), Postives = 80/82 (97.56%), Query Frame = 1 
Query:    1 GPDAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSG 246
            GPDAG+GEDSGWKQVHGDVFRAPKHLM+FSALFGTGCQL VLVL+VILFAIAGPLHGDVYEERGEMV+TFIVCYALT+ +SG
Sbjct:  273 GPDAGIGEDSGWKQVHGDVFRAPKHLMLFSALFGTGCQLAVLVLLVILFAIAGPLHGDVYEERGEMVTTFIVCYALTTLISG 354
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: W7TF26_9STRA (Transmembrane 9 superfamily member n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TF26_9STRA)

HSP 1 Score: 159 bits (402), Expect = 7.030e-44
Identity = 77/103 (74.76%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:    1 GPDAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQSSQ 309
            G + G+ E+SGWKQVHGDVFRAP HLM FSAL+G GCQ   LVLVV+ +AIAGPLHGDVYEERGE+V++ IV YALT  V+GYTSGSYYRQFF TPR+EQSSQ
Sbjct:  264 GSEHGISEESGWKQVHGDVFRAPLHLMFFSALYGIGCQFLALVLVVVGWAIAGPLHGDVYEERGELVTSVIVAYALTCLVAGYTSGSYYRQFFPTPRSEQSSQ 366
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A835YQT7_9STRA (Transmembrane 9 superfamily member n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQT7_9STRA)

HSP 1 Score: 150 bits (380), Expect = 9.580e-41
Identity = 72/96 (75.00%), Postives = 85/96 (88.54%), Query Frame = 1 
Query:   22 EDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQSSQ 309
            ++SGWKQVHGDVFRAP HLM+F+AL+GTG Q+  LV  V+LFA+AGPLHGDVYEERGEMV+T IV YALT+ V+GY SGSYYRQ+FTTPRAE +SQ
Sbjct:  270 QESGWKQVHGDVFRAPAHLMLFAALYGTGWQIATLVAGVVLFAMAGPLHGDVYEERGEMVTTVIVVYALTAAVAGYGSGSYYRQYFTTPRAEAASQ 365
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A7S4DBZ3_HETAK (Transmembrane 9 superfamily member n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBZ3_HETAK)

HSP 1 Score: 141 bits (356), Expect = 4.210e-38
Identity = 66/98 (67.35%), Postives = 81/98 (82.65%), Query Frame = 1 
Query:   16 LGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQSSQ 309
            +GEDSGWKQVHGDVFRAP+HL +F+AL GTG QL  L    +LFA+AGPLHGDVYEERGEM++  IV YAL++ V+GY SG+YY+Q F TP+AE+ SQ
Sbjct:  117 MGEDSGWKQVHGDVFRAPRHLALFAALVGTGWQLAALAAGAVLFALAGPLHGDVYEERGEMMTACIVMYALSTVVAGYASGAYYKQHFATPKAERGSQ 214
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A7S3M626_9STRA (Transmembrane 9 superfamily member (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3M626_9STRA)

HSP 1 Score: 136 bits (342), Expect = 4.900e-37
Identity = 63/99 (63.64%), Postives = 86/99 (86.87%), Query Frame = 1 
Query:   16 LGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGP-LHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQSSQ 309
            +GED+GWKQVHGDVFR P++L +F A+ GTG QL +LVL VIL+A+ GP LHG++YE+RGEM+STFI+C++L+S ++GY+SGSYYR+FF T R+E +SQ
Sbjct:  223 IGEDTGWKQVHGDVFRPPENLELFCAIVGTGWQLVILVLFVILYAMLGPYLHGNMYEDRGEMISTFIICFSLSSAIAGYSSGSYYRRFFPTARSELNSQ 321
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A7R9U3Y7_9STRA (Transmembrane 9 superfamily member n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U3Y7_9STRA)

HSP 1 Score: 133 bits (334), Expect = 9.290e-35
Identity = 62/97 (63.92%), Postives = 76/97 (78.35%), Query Frame = 1 
Query:    7 DAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAE 297
            + GLG++SGWKQ+HGDVFR P HLM+FSAL+GTG QL VL   VIL+A  G  HG+VYEERGE+  + +  Y+LTS V+GY SGSYYRQ+F TPR E
Sbjct:  136 EPGLGDESGWKQLHGDVFREPPHLMLFSALYGTGWQLAVLAFGVILYAALGRFHGEVYEERGEVTQSVLSIYSLTSVVAGYASGSYYRQYFNTPRRE 232
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A6U4JKR6_9STRA (Transmembrane 9 superfamily member n=6 Tax=Phaeomonas parva TaxID=124430 RepID=A0A6U4JKR6_9STRA)

HSP 1 Score: 131 bits (329), Expect = 1.810e-33
Identity = 62/97 (63.92%), Postives = 75/97 (77.32%), Query Frame = 1 
Query:    7 DAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAE 297
            + GL ++SGWKQ+HGDVFR P  LM+++AL+GTG QL VL   VILFA  G  HG+VYEERGEM  + +  YALTS V+GY+SGSYYRQFF TPR E
Sbjct:  289 EPGLSDESGWKQLHGDVFREPPSLMLYAALYGTGWQLAVLAFGVILFASLGRFHGEVYEERGEMTQSLLATYALTSVVAGYSSGSYYRQFFNTPRRE 385
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A7R9W337_9STRA (Transmembrane 9 superfamily member (Fragment) n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9W337_9STRA)

HSP 1 Score: 120 bits (301), Expect = 1.530e-32
Identity = 58/94 (61.70%), Postives = 73/94 (77.66%), Query Frame = 1 
Query:   22 EDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQS 303
            E+SGWKQVHGDVFRAP HL +F+AL GTG QL VL L VILFA+AGP+HG+V+EERGE++   +  YAL+S VSGY SG Y++ +  T  A+ S
Sbjct:   75 EESGWKQVHGDVFRAPSHLPLFAALIGTGWQLVVLTLGVILFAVAGPIHGEVHEERGEILHAVVWIYALSSVVSGYASGKYFKLYSATSGAKGS 168
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BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Match: A0A6U5J731_9STRA (Transmembrane 9 superfamily member n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A6U5J731_9STRA)

HSP 1 Score: 122 bits (305), Expect = 2.970e-31
Identity = 57/90 (63.33%), Postives = 73/90 (81.11%), Query Frame = 1 
Query:    7 DAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFAIAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQF 276
            +A L EDSGWKQVHGDVFR+P  L +FSAL G+G QL  ++L VILFA+AGP+HGDVYEERGE++S  IV Y L+S  +GY SG++YR++
Sbjct:   30 NAALNEDSGWKQVHGDVFRSPPFLELFSALVGSGWQLFFIMLTVILFAVAGPIHGDVYEERGEVISATIVVYVLSSITAGYYSGAFYRKY 119
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig13847.3053.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KXN7_9PHAE3.720e-5893.20Transmembrane 9 superfamily member n=1 Tax=Ectocar... [more]
D8LEY0_ECTSI6.130e-4590.24Transmembrane 9 superfamily member n=1 Tax=Ectocar... [more]
W7TF26_9STRA7.030e-4474.76Transmembrane 9 superfamily member n=2 Tax=Monodop... [more]
A0A835YQT7_9STRA9.580e-4175.00Transmembrane 9 superfamily member n=1 Tax=Tribone... [more]
A0A7S4DBZ3_HETAK4.210e-3867.35Transmembrane 9 superfamily member n=1 Tax=Heteros... [more]
A0A7S3M626_9STRA4.900e-3763.64Transmembrane 9 superfamily member (Fragment) n=1 ... [more]
A0A7R9U3Y7_9STRA9.290e-3563.92Transmembrane 9 superfamily member n=1 Tax=Pinguio... [more]
A0A6U4JKR6_9STRA1.810e-3363.92Transmembrane 9 superfamily member n=6 Tax=Phaeomo... [more]
A0A7R9W337_9STRA1.530e-3261.70Transmembrane 9 superfamily member (Fragment) n=1 ... [more]
A0A6U5J731_9STRA2.970e-3163.33Transmembrane 9 superfamily member n=1 Tax=Corethr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig13847contigH-canaliculatus_F_contig13847:695..2633 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score157.1
Seed ortholog evalue4.3e-36
Seed eggNOG ortholog2880.D8LEY0
Preferred nameTM9SF3
KEGG koko:K02639,ko:K17087
KEGG Pathwayko00195,map00195
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005794,GO:0005795,GO:0005797,GO:0005801,GO:0005802,GO:0012505,GO:0016020,GO:0016021,GO:0031224,GO:0031410,GO:0031982,GO:0031984,GO:0031985,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044444,GO:0044446,GO:0044464,GO:0097708,GO:0098791
EggNOG free text desc.secretion of lysosomal enzymes
EggNOG OGsKOG1277@2759,KOG1278@1
COG Functional cat.I
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00194,ko04147
Hectar predicted targeting categorysignal anchor
Exons3
Model size309
Cds size309
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig13847.3053.1prot_H-canaliculatus_F_contig13847.3053.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig13847 695..2633 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929125.8563726-CDS-H-canaliculatus_F_contig13847:694..7361622929125.8563726-CDS-H-canaliculatus_F_contig13847:694..736Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 695..736 +
1696845688.0923805-CDS-H-canaliculatus_F_contig13847:694..7361696845688.0923805-CDS-H-canaliculatus_F_contig13847:694..736Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 695..736 +
1622929125.8684456-CDS-H-canaliculatus_F_contig13847:1515..17181622929125.8684456-CDS-H-canaliculatus_F_contig13847:1515..1718Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 1516..1718 +
1696845688.1041656-CDS-H-canaliculatus_F_contig13847:1515..17181696845688.1041656-CDS-H-canaliculatus_F_contig13847:1515..1718Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 1516..1718 +
1622929125.8823354-CDS-H-canaliculatus_F_contig13847:2569..26331622929125.8823354-CDS-H-canaliculatus_F_contig13847:2569..2633Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 2570..2633 +
1696845688.1120484-CDS-H-canaliculatus_F_contig13847:2569..26331696845688.1120484-CDS-H-canaliculatus_F_contig13847:2569..2633Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13847 2570..2633 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig13847.3053.1

>prot_H-canaliculatus_F_contig13847.3053.1 ID=prot_H-canaliculatus_F_contig13847.3053.1|Name=mRNA_H-canaliculatus_F_contig13847.3053.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=103bp
GPDAGLGEDSGWKQVHGDVFRAPKHLMVFSALFGTGCQLTVLVLVVILFA
IAGPLHGDVYEERGEMVSTFIVCYALTSFVSGYTSGSYYRQFFTTPRAEQ
SSQ
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mRNA from alignment at H-canaliculatus_F_contig13847:695..2633+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig13847.3053.1 ID=mRNA_H-canaliculatus_F_contig13847.3053.1|Name=mRNA_H-canaliculatus_F_contig13847.3053.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=1939bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13847:695..2633+ (Hapterophycus canaliculatus Oshoro5f female)
GGGCCAGACGCAGGCCTTGGAGAAGACAGTGGCTGGAAGCAGGTGGGCCA
TAGTGGTAGCCGTAACGATGCCAGCCGCTGGTTCACCGGTTCGGTTCAAT
CTGGTTTGATCTCGTTTGGGTTGGTTTGGCGTTTTTGGTTAGGCTTGGTT
GGGCATGATTCAGGTAGATCGTGTTCTGGTTTCCGGACAGTTGTTGGGGC
ACCGCAGTGTCACCGTTCCCACGAAAGGGGAACGTGTGTTGAGGGAGACT
AGGCTTTGCTTGTGCTGCGTGAACAATTCGAGAGGCCACGCAAACTCCCC
TTCAGTCAAGATATTTACCGACGTTGATATCGACGCCCCCCGCCCCCCCG
CCGCCGTCGCCGCCACCTATCCTCCGCCACCTATCCTCCGTGCCTCCCCT
GCCTTCCGTCACCAATGAAAACAAGAATTAACGTCCGTCGTGAAAGCTGA
ATCAAATAGAATTTCAAATAAAATTGGAAACCAAAATGGCAACAAGATGT
ACCTGTCCCTTCTCAGAGAGGCTTCTTGCCGCGCGATTACGTTTTTTCTT
CGTTTTTTAAGGTCCGCGGAGACGCGTTCCAAGCGCCGAATCGAAAAAAT
TCGAAACGAATGAAGAATGGTGCTTTTATCCCGTCCAATCAACTGAACCC
TGACTGCACGGAACCCAACGCAAGCCTCCTGAGACACCTGGGGAGAGGTG
TCAGCGGGAATTGCTTTCGTACGTCTTGTCGACTCTTTTATTGGAAATGA
GCTGAAAGATCCACAAGTACCATATCAAAAGACAGTAACCTTCGCTCTTC
TTTGCTTGTTCTTTGTAAAAGGTCCACGGGGACGTGTTCCGAGCACCGAA
GCATCTCATGGTCTTCAGCGCGCTGTTCGGCACGGGCTGCCAGCTGACGG
TGCTGGTGCTGGTGGTAATCCTCTTCGCGATCGCGGGGCCCCTCCACGGC
GACGTGTACGAGGAGCGAGGGGAGATGGTGTCCACGTTCATCGTCTGCTA
CGCGCTCACGTCCTTCGTCTCGGGGTATGTTGGGTCTGTGACCGTTGTGC
TTGTCGTCGGTGTGTTTCGTGTGTGTGTCTTTGTGCGGTTTCCTCCGCAT
GTCTGTGCGTGTGGGTTTGTTTGTGTGTGTGTCCATATGTGCGCTCGTGT
GCGTGGGCTCTGCGGGTGTAACTTCCGCTTCGCCCGTTTTTGTGCGCTTC
GTGTGGCTGTGTGCTTTTCTGTGTTTTTTTGTCGGTCCCTTCGTGCGTGT
TTTGCTTTGGAACCACGGTTGTGCCAAGCACCGGTATATATATGCATCAT
GTGCTCGGCGTGCCGTCGATTAGATCGATGAGATCGCAGTGGTGATTTGG
CGTGCTTGTCGTGCAAACTCGGTGGCTTCCTTTGCGCTGAAAGTGGGGGC
GGGGAATTTCGAAGAAGTGTGTTCGGTAGGGGAGGGTCTGGGCAGCAGGT
GCTCGAAATGTGGCAGACGGTGTTTACATCATGGAGGCTGTTTTTGCTGG
CGTTTTTAGTGTTGATGGGCGTGATTTGTTGAGCTTGACTTCGTAACAGA
CGCATATCTTGATGGTGATTAGTGCTCTCTCTTACTGCGCTGATTCTTCT
CACGGATGATGGTGACCTTTTTGTTTTTGTCTATATCGCTGCCGTTCGAC
AGTTGTTCTTGTTGTTGTTGCTGTCATTCTCGCCGTTGCCGTCACCATCT
CAGCTATTGTGATCATCATCATCATACTTGACATCGACGTTTTTGTTGTT
GCTTTCATCCTTACAGAGGTCACGTTCGTCCTCTCCCGCCACATTCGTCT
CGTACACAACTTGGCGCGAATTTGTGTCGCCCGCTGCCAACTGATTCCCT
CCCACTCAAATTTGATTTCGGACAGGTACACGAGCGGGAGCTACTACCGA
CAGTTCTTCACCACCCCGCGTGCGGAACAAAGCTCTCAG
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig13847:695..2633+

>mRNA_H-canaliculatus_F_contig13847.3053.1 ID=mRNA_H-canaliculatus_F_contig13847.3053.1|Name=mRNA_H-canaliculatus_F_contig13847.3053.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=618bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13847:695..2633+ (Hapterophycus canaliculatus Oshoro5f female)
GGGCCAGACGCAGGCCTTGGAGAAGACAGTGGCTGGAAGCAGGGGCCAGA
CGCAGGCCTTGGAGAAGACAGTGGCTGGAAGCAGGTCCACGGGGACGTGT
TCCGAGCACCGAAGCATCTCATGGTCTTCAGCGCGCTGTTCGGCACGGGC
TGCCAGCTGACGGTGCTGGTGCTGGTGGTAATCCTCTTCGCGATCGCGGG
GCCCCTCCACGGCGACGTGTACGAGGAGCGAGGGGAGATGGTGTCCACGT
TCATCGTCTGCTACGCGCTCACGTCCTTCGTCTCGGGGTCCACGGGGACG
TGTTCCGAGCACCGAAGCATCTCATGGTCTTCAGCGCGCTGTTCGGCACG
GGCTGCCAGCTGACGGTGCTGGTGCTGGTGGTAATCCTCTTCGCGATCGC
GGGGCCCCTCCACGGCGACGTGTACGAGGAGCGAGGGGAGATGGTGTCCA
CGTTCATCGTCTGCTACGCGCTCACGTCCTTCGTCTCGGGGTACACGAGC
GGGAGCTACTACCGACAGTTCTTCACCACCCCGCGTGCGGAACAAAGCTC
TCAGGTACACGAGCGGGAGCTACTACCGACAGTTCTTCACCACCCCGCGT
GCGGAACAAAGCTCTCAG
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