mRNA_H-canaliculatus_F_contig1299.2473.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: D8LK68_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK68_ECTSI)

HSP 1 Score: 811 bits (2095), Expect = 3.860e-255
Identity = 655/2182 (30.02%), Postives = 861/2182 (39.46%), Query Frame = 2

Query:   92 SSLDRSILCELFEKTGGVESWRKKDGWATARPMSEWYGVETNSEGRVISLDLAYNSLEGQLPSGLGELKFLKTLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQLGAEAKTTLGTADSDAG-KMGHLENCILFPDLNAKGWESGDVDEECHHWTSGPLKASQELRHVSFWWKDQGWGNRKGMLYLSLDPHPSIEQEESDHKKKGHRQLTPEPAEHDWTRAVVKVDPEDPLRRLAQTGDRYRLSYLAGGGGGHEIRVAGFKLHLGAAGGHRGDEIFSSVLWKLDLSGNQLEGSIPMEIANLSGLELLKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTELPKDLASEWASMKLHETR-LGLFLDGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPAEKRTVGVEVTEWKPREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPP--GLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSC--------------------------------------------------------------------------GLDDSXXXXXXXXXXXXXXXSP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKEDFSFPSVGAIVPPAWMDAIAAVEALGQERGNPFVMWDDALKSFQSHYKNKS--------NKPPGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLD-EGNYEGVDLN-GCFKNMLDAMIRYLFIYRAPEPSNNGQEVYVVPARLPLYGKENVLAENIGLGEAVEGIKAWFIRTHLPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENK----GTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENISNYSVRIFKNSFPSYFDPEKQPYPRLVVLKPVAED--QEASTGEHDEGTIHSREMWNRWARLWKSRRAGDSC--YLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFGEFEEHLPRDFDADPTGFNVDATWEEGQSGRFKELHEPAYEKLCEFMKKEEGSKGACPC-----LHRFLCGKTSTQPKNWRDEMEQVKHGNGEWAWVRRS 4060
            +S DR +L  L   TGG  SW     W T  P+ +W+GV+   +G V+ LDLA N+L G +P+ LG+LK LKTL                               XXXXXXXXXXXXXX                   QLG   +T +    +DAG + GH       PDL A+G  S D ++E  HW+S PL+   ELR+VSF W+DQGWGNRKG L+LSLDP  S +Q+ S  + K HRQL P+PAEH WT  V+++DP+DPLRRLA  GDRYRLS++AGGGGGH ++   F LH   AGG  GDE++S VLWK+DLS NQL G+IP +++NL  L +L+  XXXXXXXXXXXXXXX             + LP DL ++WA     + R +G+ ++GNPWVRPP   L++GL +  +WW  IA FGEGTS+KLK+VLVGLA+AGKTT+VRHFTG  PA+KRT+GVE++EWKP + LPL+VSLWDF+GQS+Y++SHQIFLT+GALFLLVVD+FEL RD    G+ DPRGAI+RWL ILH+RVPGAVV LVGTHGDKFF +  P S S+                                                                               GLDD                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GQ+QL  AEKRF  LVR YQ ++AKE K             LS   AS + ++  QR S                       PL +  ++F  S+   N +  L DWI+  A EK  FSFP+V  I+P AW+DAIAAVEAL +E+G P+V+W DA+++FQ +++ K         NKP  D +  + L +A+ HREAEGG++LS+ D SSPA+ D+IH+DPRWL+ELV+R+ DHNLVD++KQ +I +EL+ Y+ TR  T  G+  L + H  FIRQGRL+++YLRFLWVHRKL+ EG+Y G  L+   F +M+D MI+YLFIY +    +N ++ ++VPARLP +G +N+L E+IGLGE V G  AWF+R+H P+GIIGRFLAFT+    +SG CWQHGAHI+WKKD++ +DVLL ETT  K    G  T+PAI++ VK + ++A  VL E+ E+L SL++DN+HGYPG+ LP F+++    S++ Q H   YL TQ   L KILE+    SVR+F+ +FPSY DP + PYPRLV+LKPV     QE    E   G I  R  W+RW  L +S   G SC   LVFLC  DFSEIPCGP+GKG+ + NPG L  ++ PLLQGALWL+++ +  V+++DLPL+D+  A + +   E  E       A P+ F+  AT +EGQ   FKELH PAY+ LC+FM KEE       C      +R    +      +WR  ME +K+G G ++WVR+S
Sbjct:    2 ASTDRDVLLVLHRSTGGA-SWTNNANWDTDAPIGDWHGVKVKDDGHVVKLDLASNNLRGDIPAILGKLKLLKTLILSDNTLTAKPGGAQPQLLWSALETRTSKGSXXXXXXXXXXXXXXDLGSNELSGHIPDRLG---QLGVSIRTNVEENFTDAGNRSGHQ---TFVPDLYAEGIGS-DPEDERDHWSSEPLEPLHELRYVSFLWRDQGWGNRKGTLFLSLDPG-STDQQCSTQEPKEHRQLMPDPAEHKWTHEVMEIDPKDPLRRLACAGDRYRLSFIAGGGGGHILQARRFTLHFSVAGGSCGDELYSRVLWKIDLSNNQLTGNIPQDLSNLRDLGVLRLGXXXXXXXXXXXXXXXERLFDVDLTNNELSVLPMDLTAKWALAVQDDNRKVGVSVNGNPWVRPPRAFLARGLVSAGRWWDGIARFGEGTSSKLKMVLVGLAEAGKTTIVRHFTGGDPADKRTIGVELSEWKPNKNLPLAVSLWDFSGQSDYHASHQIFLTEGALFLLVVDLFELGRDEKSAGIPDPRGAIYRWLVILHERVPGAVVALVGTHGDKFFPRPPPSSESAGDEDRENRIDVLNTLLKILAGLLRQERAKLGGQLSADKHLGNLLQLVRTQVEPRLSADDLXXXXXXXXXXXXXXXWADEGLDDLMRLVQTTLVGQHSDEKSKFEXXXXXXXXXXXXXXXADDLCDVLQWVQTRXXXXXXXXXXXXXXXVQLVRTNLQXXXXXXXXXXXXXXXXXXXXXXXXXGGLAYGLXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGHXXXEGLGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLADGLMELFASKMVIQLPADDLGDVLQWLRARLEGHLSGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEDRADSLVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGLGDLVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSDADRADGLEELVHSKMEFQLXXXXXXXXXXXXXXXXXXXXSGEGLGDVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADVLVELVHSKLXXXXXXXXXXXXLRWLRARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSDVDLAGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSAEGLGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGTDLADGLLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQRRAEDLDDLVRLXXXXXXXXXXXXXXXXXXXXXXXXXXXXILGDEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRLQLSGXXXXXXXXXXXXXXLEDQVVSAEDLDDLVQLMRIYLQDQLPAXXXXXXXXXXXXXXXXXXXGPRYAEEIKNGQRQLYKAEKRFRTLVRQYQEDKAKELK-------------LSCGNASAEKDDSFQRGST---------------------HPLILHPRVFCASLGLDNRIGRLSDWIIGVAPEKISFSFPAVATILPAAWVDAIAAVEALHKEKGTPYVLWGDAVRAFQRYFQEKHKEEGKEERNKPLSDEDAATNLKEAMKHREAEGGVILSLRDESSPAQSDMIHVDPRWLIELVRRVTDHNLVDKKKQNKILQELLEYDRTRPDTLSGYKNLCETHACFIRQGRLNRDYLRFLWVHRKLEVEGDYAGAQLDKDAFDSMVDTMIKYLFIYPSHGECSNAEDCHIVPARLPEHGNDNILEESIGLGEVVVGKTAWFLRSHAPTGIIGRFLAFTSSRFEASGKCWQHGAHIQWKKDSNEHDVLLCETTIEKDIIPGQSTFPAIAICVKGKTAEAKGVLREVGEQLLSLLQDNIHGYPGLRLPIFEELNITQSDQFQVHFERYLATQ---LAKILEDTRRESVRMFRAAFPSYGDPVELPYPRLVLLKPVETTSIQETQAEESVNGAIQLRRTWDRWIDLCRS---GGSCDFVLVFLCERDFSEIPCGPQGKGFRVHNPGSLFNTLKPLLQGALWLVKIAVGTVASVDLPLHDVLEAFLHATAAEVLE-------AAPSQFSPSATLDEGQQEGFKELHGPAYKALCDFMTKEEFGPEEARCESCLPWYRPRVHEPIPSEFDWRSHMEMIKNGIG-YSWVRKS 2126          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A6H5JR01_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JR01_9PHAE)

HSP 1 Score: 529 bits (1362), Expect = 8.570e-166
Identity = 301/670 (44.93%), Postives = 409/670 (61.04%), Query Frame = 2

Query: 2117 PLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKEDFSFPSVGAIVPPAWMDAIAAVEALGQERGNPFVMWDDALKSFQSHYKNKS--------NKPPGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLD-EGNYEGVDL-NGCFKNMLDAMIRYLFIYRAPEPSNNGQEVYVVPARLPLYGKENVLAENIGLGEAVEGIKAWFIRTHLPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENK----GTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENISNYSVRIFKNSFPSYFDPEKQPYPRLVVLKPVAEDQEASTG-EHDEGTIHSREMWNRWARLWKSRRAGDSC--YLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFGEFEEHLPRDFDADPTGFNVDATWEEGQSGRFKELHEPAYEKLCEFMKKEE-GSKGA--CPCL--HRFLCGKTSTQPKNWRDEMEQVKHGNGEWAWVRRS 4060
            PL +  ++FR S+   N V  L DWI++ +TEK  FSFP+V  I+P AW                  V+W DA+K+FQ +++ K         NKP  D E  S L +A+ HREAEGG++LS+ + SSPA+ D+IH+DPRWL++LV+R+ DHNLVD++ +  I +EL+ Y  TR  T  G+  L + H  FIRQGRL+++YLRFLWVHRKL+ EG Y GV L N  F +M+D MI+YLFIY +    +  +  Y+VPARLP  GK+NVL E+IGL E V G  AWF+R+H P+GIIG FLAFT+    +SG CWQHGAHI+WKKD++ +D+LL ETT  K    G  T PAI++ VK + ++A  VL E+ E+L SL++D +H                       H   YL T+   L  ILE+    SVR+F+ +FPSY +  +  YPRLV+LKPV       T  E   G    R  W+RW  L  S   G SC   LV LC  DFSEIPCGP+GKG+ + NPG L  ++ PLLQGALWL+++ +  V+++ LPL+D+  A + +   E  E       A P+ F+  AT +EGQ   FKELH PAY+ LC+FM+KEE G + A  CPCL  HR    +      +WR  M ++K+G G ++WVR+S
Sbjct:   42 PLILHPRVFRASLGLDNRVNRLSDWIIDVSTEKISFSFPAVATILPSAW------------------VLWGDAVKAFQRYFQEKHKEEREDERNKPLSDEEASSNLKEAMKHREAEGGVILSLREESSPAQSDMIHVDPRWLIDLVRRVTDHNLVDKKNEHEILKELLEYGRTRPDTLSGYKNLCETHTCFIRQGRLNRKYLRFLWVHRKLEVEGGYAGVQLENDTFDSMVDTMIKYLFIYPSHGECSKAEGFYIVPARLPEIGKDNVLEESIGLDEVVVGKTAWFLRSHAPTGIIGCFLAFTSSRFEASGECWQHGAHIQWKKDSNEHDMLLCETTIEKDIIPGPGTLPAIAICVKGKTAEAKGVLREVGEQLLSLLKDVIH----------------------VHFERYLATK---LATILEDTRRESVRMFRAAFPSYGEHVELLYPRLVLLKPVETTSIQETQVESVSGAKKLRRTWDRWIDLCWS---GGSCDFVLVILCERDFSEIPCGPQGKGFRVHNPGSLFNTLKPLLQGALWLVKIAVGTVASVYLPLHDVLEAFLHATAAEVLE-------AAPSQFSPSATLDEGQQEGFKELHGPAYKALCDFMRKEEFGPEEAQCCPCLPWHRPRVHEPILSEFDWRSHMYKIKNGVG-YSWVRKS 657          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: D8LKS5_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LKS5_ECTSI)

HSP 1 Score: 516 bits (1330), Expect = 4.680e-155
Identity = 355/983 (36.11%), Postives = 522/983 (53.10%), Query Frame = 2

Query: 1286 NPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPAEK--RTVGVEVT-EWKPREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRD----PPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRS----YQAERAKEDKVLRNYAQSRAEGELSSRKASK--DAEEGRQRASAGLRRTPGSFRMPP-ARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKE---DFSFPSVGAIVPPAWMDAIAAVEALGQERGN-PFVMWDDALKSFQSHYKNKSNKPPGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDE--RKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLDEGNYEGVDLNGC-FKNMLDAMIRYLFIYRAPEPSNNGQEVYVVPARLPLYGKENVLAENIGLGEAVEGIKAWFIRTHLPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRT-----WPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFS-----------------NLTKILENISNYSVRIFKNSFPSYFDPEKQPYPRLVVLKPVAEDQEASTGEHDEGTIHSREMWNRWARLWKSRRAGDSCYLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFGEFEEHLPRDFDADPTG----FNVDATWEEGQSGRFKELHEPAYEKLCEFMKKEEGSKGAC-----PCLHRFLCGKTSTQPKNWRDEMEQVKH-GNGEWAWVRRSNLKSF 4075
            NPW RPPSTVL  G +    WW D+  FGEG SNKLK+VLVGLA AGKTT+VRH TGR   ++  RTVG+E+T +WKP +E+PL VS+WDFAGQ++YYSSHQ+FLT+GALFLLVVD+   S++         DPR  I+ WL++LH RVPGA V LVG+H D                                         Q + EDA +    L       +   R     V+  + + +A G  S R++ +  DAE  +      LR      RM P +   + T +PL + G +F+ S+    +V EL+ WIV+AA+ +E    F+FP+V   V  AW++A  A++ L   +G  P V+W  A++ F+   +  S  PP   +   +L++A+ HREAEGG+LLS+ D S+P   D++HLDP WL+ELV+R+ DHNLVD+  +K+G + + L  Y + +G      + L+  H  + R GRL++ YLRFLW+HRK+ EG    VDL    F++++  M R L +Y A +  N      VVPARL  YG + +      L   V  I+  F + + P GI+GRFLA++A+ +   G CWQHGA   +K     YDV LYE+   +  +      +  +++ ++    +A++VL EL + L+SL+ D+ +GYPG+  P +        + L   L   LD                     +L + +  +     ++ +       D E+  YPRLV+++P  E  E  T E  +     R  W+RW +   S        + FLC +D SE+PCGP G+G+ I++P + V+  LPL+Q +LW+LRV +  VS +DLPLN +  A V +   E  E + +  D   +     F+  AT    +   FK+L   +YE LC+FM+  E S   C     PC HR L         +WRD M QV++   G  AWV ++N K+F
Sbjct:  248 NPWSRPPSTVLESGFERALGWWEDVKRFGEGKSNKLKMVLVGLAGAGKTTIVRHMTGRSVPKRSDRTVGIEITTDWKPGDEVPLQVSVWDFAGQADYYSSHQLFLTKGALFLLVVDLHAFSKEIGSGVDNFTDPRRRIYWWLEMLHMRVPGAAVALVGSHVD-----------------------------------------QMESEDARQAETHLHTGEASWFFRGRVPCSLVVSKFIKDKA-GNASRRQSIRCIDAESTKPMEVDHLR---DDGRMAPRSGSSRFTAEPLVLHGNVFKVSL-APTSVVELRQWIVKAASGRECPTGFNFPAVDQTVSKAWIEAYDAMDVL---KGTAPCVLWSRAVEEFR---RKMSGIPPVAGDAGKVLLRAMQHREAEGGVLLSLADDSAPVASDMLHLDPSWLIELVRRLADHNLVDKDKKKRGSLEQGLRVYAKRQGLAA---APLFDIHNVYCRSGRLNRAYLRFLWMHRKI-EGPGPTVDLTDPEFESIVSTMERLLVMYTARDADN-----LVVPARLQEYGDQRIPDR---LTNMVLKIECSFGQVYPPPGIVGRFLAWSAQQVADYGECWQHGAFFSYKYQYARYDVFLYESEVEEAHQDGTVDMFAGLTLGIQGPPPQAAEVLEELRKSLESLVSDSAYGYPGLVGPMYFRPAEEIESSLLVRLGSVLDXXXXXXXXXXXXXXXXXXXXXSLEETVRKLGGIVTQLLEQDLLPASD-EQSAYPRLVLVRPELETAEDMTHERIQ-----RAGWDRWTKA-LSEMGFHKFRVHFLCGHDLSEVPCGPGGRGFPIKHPKDWVKQWLPLMQVSLWMLRVAVGAVSNVDLPLNKVLGAFVMATGAELLEGVGKVPDRISSAALPVFSEGAT--RAEMDGFKDLRGKSYEGLCKFMRDVESSPPQCLSGCLPC-HRTLA------VVDWRDHMVQVRNPSTGRRAWVLKTNEKAF 1150          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A6H5JPJ1_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPJ1_9PHAE)

HSP 1 Score: 257 bits (657), Expect = 4.750e-66
Identity = 257/950 (27.05%), Postives = 407/950 (42.84%), Query Frame = 2

Query: 1265 LGLFLDGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTG---RQP---AEKRTVGVEVTEWK---------PREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNV--AELKDWIVEAATEKED-FSFPSVGAIVPPAWMDAIAAVEALGQ-----------------------ERGNPFVMWDDALKSFQSHYKNKSNKPPGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGD--------VIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLDEGNYEGVDLNGCFK-------NMLDAMIRYLFIYRAPEPSNNGQEVYVVPARLPLYGKENVLAENIGLGEAVEGIKAWFIRT-------HLPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCL--------------PAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENIS------------------NYSVRIFKNSFPSYFD-------------PEKQ-PYPRLV-VLKPVAEDQEASTGEHDEGTIHSREMWNRWARLWKSRRAGDSCYLV-FLCAYDFSEIPCGPEGKGYLIQNPG--ELVQSMLPLLQGALWLLRVGIKIVSAID-LPLNDLSSALVQSA 3772
            + ++   NPW  PP+ VL  G+    ++ +D+ +FG  +SN+LKVVLVGL  AGKT+V     G    +P   AE+RTVGVE+ + +          R    L V LWDFAGQ  YY +HQ+FLT GALF+LVVD+F  S D     D   A+ +WLDIL  RVPG+VV LVGTH D F           S + C   D                              +D V        +E K  R  A+  + G                R  AG++    S R  P R++        +  ++   ++     V  A+L++     A    D +SFPSV + VP   + A+A +EA+ +                       + G PF+++ +AL  F      KS       E +   + A+   E +G IL++  D     E          VIH++P W  +L++RI+D  L+D  +Q ++ E +      +  +P   + L K H  F   G + + Y++FLW  R L+ G          F+        M+++++   F++R      + ++ Y+VP+ LP +    V A+ + LG  V G  A F R         +P G+I R LA+ ++       CW+ G    + +    + VLLYE  +  G R+             KA   L ++  +L+ L+ D  +G+PG+ L                 D +    + EL++H+    +  F  L +    I+                  N+ ++          D              EK  P PRL+ VL+ V+     S   H       R+M ++  R  +       CY + FLCAYD +   CG +G GY ++  G  E +++ LP+++ +LWLLRVGI +V+  D LP++ +   L + A
Sbjct:  178 VSIYTAENPWTEPPADVLELGMAYAARFLKDLNDFGRTSSNRLKVVLVGLGNAGKTSVAVRLEGLASSKPLPTAEERTVGVEIRDIQLAPGPANGGSRGNAELDVKLWDFAGQRAYYDTHQMFLTPGALFVLVVDIFAYSEDGHSRED---ALEQWLDILQARVPGSVVLLVGTHSDMF----------DSPADCSKRD-----------------------------IMDRV-------GRECKSARERARQESAGNSD-------------REYAGVQ---DSRRYQPLRIV--------VVDELLALNLTPSGGVTTAQLQEHFEGVAYGVHDGYSFPSVNSEVPEPHLLAVATLEAVRRGATLRGSGGDPAEVVRRLDGEKLDEGQPFILFPEALSLFLEVAGEKSLFGGNATEGEQAFLAAITLHERQGAILVTRADGGGEKEDMKPASEQHLVIHVNPAWFADLIRRIVDVRLLDPAQQEKLAEVI------KRQSPASVAELSKQHGRFFLAGEVSKRYMKFLWKERDLELGPASPSLAAPPFEMSEETITTMVESLLAVRFMFRV----RHNKDHYIVPSCLPDHVGCTVDAKKL-LGLDVGG--AIFSRKLQLGGEQPVPPGLIPRLLAWCSQGDARITTCWKRGVCFAFNRK---HLVLLYEHRKEAG-RSVIECHAMGSAHNEKAGGTLDDVVAELRRLVGDPKYGFPGIWLFFDGGVEHQKATSAGELDKLLSRFAGELKDHM----NVNFKELARKSNVIAGGVERHLRKGQEEMATSFNHQIQQLGQDMSRKLDCIAAVTSKCLLVSVEKDIPCPRLIMVLEDVSPGSALSRLSH-----FRRDMVDKLLRR-EGEGPAPQCYRIRFLCAYDLTAGTCGKDGLGYKVEIQGWQEWLKACLPVIKVSLWLLRVGISVVTRADVLPVDGVIETLEEVA 1027          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A6H5JSN1_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSN1_9PHAE)

HSP 1 Score: 253 bits (647), Expect = 8.530e-66
Identity = 270/932 (28.97%), Postives = 402/932 (43.13%), Query Frame = 2

Query: 1286 NPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQP------AEKRTVGVEVTEWK----PRE-----ELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNV--AELKDWIVEAATEKED-FSFPSVGAIVPPAWMDAIAAVEAL---------GQE---------------RGNPFVMWDDALKSFQSHYKNKSNKPPGDIEPKSILIQALAHREAEGGILLS-------MEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLDEG----------NYEGVDLNGCFKNMLDAMIRYLFIYRAPEPSNNGQEVYVVPARLPLY---GKENVLAENIGLGEAVEGIKAWFIRTH-LPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCL-----------PAFDDIRGLTSN---ELQEHLIPYLDTQFSNLTKILENISNY------SVRIFKNSFPSYFD-------------PEKQ-PYPRLVVLKPVAEDQEASTGEHDEGTIHS--REMWNRWARLWKSRRAGDSCYLV-FLCAYDFSEIPCGPEGKGYLIQNPG--ELVQSMLPLLQGALWLLRVGIKIVSAID-LPLNDLSSALVQSA 3772
            NPW  PP+ VL  GL     + +D+ +FG  +SN+LKVVLVGL  AGKT+V     G  P      A++RTVGVE+ + +    P          L V LWDFAGQ  YY +HQ+FLT GALF+LVVD+F  S D     D   A+ +WLDIL  RVPG+VV L+GTH D F   A  CS                                       KR    V+           + +N+   R   E    KA+ D      R  AG++    + R  P RV+        +  ++   ++     V  AEL++ I   A    D +SFPSV   VP   + A+A +EA+         G E                G+PFV++ +AL  F      K        E   + + A+   E +G ILL+       MED +S  E  VIH++P W  +L++RI+D  L+D     +  +E VA    R P P     L K H  F   G L++ Y+RFLW  R L+ G               D+    +++LD  +R++F  R  E      + Y+VP+ LP +     +      + +G A+   +  F+  H +P G+I R LA+  +      ACW+ G    +K+      VLLYE  +    R              KA   L ++  +L  L+ D  +G+PGV L            + + + GL +    EL+E +   ++     L  I   +  +       +   +       D              EK  P PRL++   V+ED      E D+  +    REM ++   L+  +      Y + FLCAYD S   CG +G GY ++       +Q  +P++Q ALW +RVG+ +VS  D LP++D+   L + A
Sbjct:   13 NPWTEPPADVLQVGLAYAASFLKDLEDFGRTSSNRLKVVLVGLGNAGKTSVAVRLEGLAPSKPLPSAKERTVGVEIRDIRLGSGPANARSGGNAELDVKLWDFAGQRAYYDTHQMFLTPGALFVLVVDMFAYSEDGHSRED---ALEQWLDILQARVPGSVVLLMGTHSDSFDSPAE-CS---------------------------------------KRVDCFVKGELLP------LYKNFIMKRIGDEC---KAAAD------RGHAGVQ---DNRRYEPLRVV--------VVKELLALNLSPSGGVTTAELREHIEGVAYGVHDGYSFPSVNNEVPEPHLLAVATLEAVRRGVSLRGSGGEPAEVARRLNAGTKVGEGHPFVLFPEALSRFLEVAGEKPMFGGKATEGARVFLAAIKLHEVQGAILLTRADGGGEMEDMASE-EHLVIHMNPAWFADLIRRIVDVRLLD---PAQARQEKVADLIKRQP-PASVGDLSKQHHRFFLAGELNRRYMRFLWKDRDLELGPASPNTAPPLEMSEEDITTIVESLLD--VRFMFRVRQNE------DHYIVPSCLPDHVGCDMDPKKLMELEVGGAIFSRRLEFVGAHSVPPGLIPRLLAWCGQGDERITACWKRGVCFSFKEHL----VLLYEHRD--AGRAVIECHAMGSAHDEKAWRTLEDVVTELGQLVGDAKYGFPGVGLFFVGGGDKQKVSSAETLEGLLTRFTGELKEQINVKVEELGQKLDDIAGGVEKHLRKGQEEMASLREDVSLQLDCIAAVTSECLLVSAEKDIPCPRLIM---VSED---DVPESDQSRLSHFRREMMDQ---LFLGKGQTPQHYRIRFLCAYDLSAATCGEDGLGYKVEIGSWKGWLQKYMPVIQVALWTVRVGVSVVSRADILPVDDVMGKLREVA 847          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: D7FU35_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU35_ECTSI)

HSP 1 Score: 253 bits (647), Expect = 1.080e-64
Identity = 288/1036 (27.80%), Postives = 437/1036 (42.18%), Query Frame = 2

Query: 1280 DGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPA-----EKRTVGVEVTEWK----PREE-----LPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARV-MKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKED-FSFPSVGAIVPPAWMDAIAAVEAL---------GQER---------GNP-----FVMWDDALKSFQ----SHYKNKSNKPPGDIEPKSILIQALAHREAEGGILLSMED--------------RSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVH-RKLDEGNYEGVDLN---GCFKNMLDAMIRYLFIYRAPEPSNNGQEV-----YVVPARLPLYGKENVLAENI---GLGEAVEGIKAWFIRTH-LPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRTWPAISVFVKCE-RSKASD------VLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENISNYSVRIFKNSFPSYFDPE--KQPYPRLVVLKPVAEDQEASTGEHDEGTIHSREMWNRWARLW--KSRRAGDS-----CYLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFGEF----EEHLPRDFD--ADPTGFNVDATWEEG----------QSGRFKELHEPAYEKLCEFMKKEE--GSKGACPCLHRFLCGKTSTQPKNWRDEMEQVKHGNGEWAWVRRSNLKSF 4075
            + NPW  PP+ V+++G+     + RD+ ++G   SN+LKVVLVGL +AGKT++      R  +     E+RTVGVE+ + K    P  E     + L VSLWDFAGQ  YY +HQ+FLT GALF+LVVD+F  +           A+ +WL+IL  RVPG+VV LVGTH D F   A+ C                                                + + +R K+D V     + R E + +  +   +  EG    +  L+   G+ R  P RV +K     L +      TS D ++ +  L+  +   A    D +SFPSV ++VP  ++ AIA +EA+         G+ R         G+P     FV + +A+  F     S +   SN      EP+ I + A+   EA G ILL+  D               ++ A   +IH++P    +LV+R++D  LVD R+Q ++ E + A    R         L   HK F++ G + ++YL+FLW+    L E + E   L       + M+ +++   F+YR      +GQ       YVV + LP     NV    I    +G A+   K   +  H +P G++ R LA+         ACW+ G    +K       VLLYE     GT +W      ++C  R   SD       L ++ +++  LI D+ +G+PG+ L        + S E +E +I       + L +I++ + +     F        D    + P PRLV + P  E +     E D     S E W+   R W    +R G         + FLC YD S   CGP G+GY +       +  +P  +    L  + +++   + +P  D  + L   A G      EE L    +  A   G  +D    EG          Q G    +    YE L E +K  E  G +G  P        K     +  R E         EWAWVR  N+  F
Sbjct:  319 ENNPWKEPPAEVMNKGMAHAAVFLRDLDDYGRAWSNRLKVVLVGLGEAGKTSIATRLEDRLGSSCPKPEERTVGVEIRDIKLGPGPTNEGSGPNVELDVSLWDFAGQRAYYDTHQMFLTPGALFVLVVDMFTYTEGQ----SREDALEQWLEILQARVPGSVVLLVGTHTDLFAENAAEC------------------------------------------------TRRTDRFKKD-VEEIMTRLRCECDSAKDRVETELGEGH---TTDLK---GNPRYQPLRVVLKEELLALDL------TSSDGQD-IDLLQQRLEHLAYNGYDGYSFPSVRSVVPEPYLPAIATLEAVRRGADLRGSGRTREAVAQRLGEGDPKKRRSFVRFSEAVSLFTKQKGSMFSRVSNLFRRAGEPRRIFLAAVKSHEAHGAILLTGVDGGGGSQEQHNTGIGAAASAADLIIHVNPSRFADLVRRVVDVRLVDPRQQAKVEEAMEACTSVRPM----LLTLTDQHKRFVKAGEVSKDYLKFLWLRDMDLGEASQEAPPLEMSEADVEVMVGSLLDVRFMYRV----RDGQHAFVPDRYVVASCLPNKAGLNVDPGKILELKMGSAIYSQKLKLVGAHAVPPGLVPRLLAWCGRGEGRIKACWKRGVCFAFKNHL----VLLYEVRAIDGT-SW------IECHARGNVSDDDSARTALKDIGDQVHELINDHKYGFPGLGL--------VRSGESEETVISSDSDHKALLERIVDVLRDQMNVKFMMDRSMRQDVAFLRWPIPRLVCVLPAPESRAKPLEEKD----RSFEQWSATLRNWCVDGKRTGKGFATRKLRVFFLCPYDRSLAECGPGGQGYEVTELLGWAKKAMPFAEVGRALASIALRVCIGLAIPEVDFEATLGTVAGGALSKFAEESLNSGIESVASIAGERLDGGRAEGLPQGAGAHGAQQGWPSPVQGFTYETLKEVIKSFEVDGMRGRSP--FPSFDAKMHLVDRGGRGE---------EWAWVRTKNIDQF 1246          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A6H5K9A9_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9A9_9PHAE)

HSP 1 Score: 232 bits (592), Expect = 8.790e-59
Identity = 259/949 (27.29%), Postives = 397/949 (41.83%), Query Frame = 2

Query: 1214 PKDLASEWASMKLHETRLGLFLDGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPA-----EKRTVGVEVTEWK---------PREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKED-FSFPSVGAIVPPAWMDAIAAVEA--LGQE----------------RGNP-----FVMWDDAL-----KSFQSHYKNKSNKPPG---------------------DIEPKSILIQALAHREAEGGILLSMED--------------RSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVH-RKLDEGNYEGVDLNGCFKN---MLDAMIRYLFIYRAPEPSNNG-QEVYVVPARLP-LYGKENVLAENIGL--GEAVEGIKAWFIRTH-LPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENISNYSVRIFKNSFPSYFDPEKQPYPRLVVLKPVAEDQEASTGEHDEGTIHSREMWNRWARLW--KSRRAGDSCY-----LVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFG 3778
            P+ +A E+A+     + + +  D NPW  PP+ V+ +G+     + RD+ ++G   SN+LKVVLVGL  AGKT++      R        E+RTVGVE+   K             + L VSLWDFAGQ  YY +HQ+FLTQGALF+LVVD+F  +        P  A+ +WLDIL  RVPGAV+ LVGTH D F   A+ C+  +                            ++ +E   KR      S Q + AK          + AE EL  R    D E              G+    P RV+      L++      TS D  + +  L+  +   A    D +SF SV ++VP  ++  IA +EA  LG +                 G+P     FV + +A+     +  +S     SN                         D E + I + A+   EA G ILL+  D               ++ A   +IH++P    +LV+R++D  LVD R+Q ++ + + A    R         L   HK F++ G + ++YL+FLW+   +L + + E   L     +   M+ +++   F+YR  +  +    + YVV + LP   G E    + + L  G AV   K   +  H +P G++ R LA+         ACW+ G    +K     + VLLYE  +  G  +W             A   L ++ +++  LI D  +G+PG+ L   +    L S++        L  +  N+ +   N+     R  K          + P PRLV + P  E       E D     S + W+   R W    +R G         L FLC YD S   CGP G+GY +       +  +PL +  L L     K+ + + +P  D  + L   A G
Sbjct:    7 PRLVAEEFAA-----SSVTVDTDNNPWKEPPADVMKRGMAYAAVFLRDLDDYGRAWSNRLKVVLVGLGGAGKTSIATRLEDRLGGCCPNPEERTVGVEIRNIKLGPGATSEGSGPNVELDVSLWDFAGQRAYYDTHQMFLTQGALFVLVVDMFAYAEGH----SPEDAVEQWLDILQARVPGAVLLLVGTHTDLFEDNATECTKRT-------------------------DRFKKDVEKIMKRI-----SCQCDSAK----------NLAETELGERHP--DLE--------------GNPHYRPLRVVLEEDL-LTLD----LTSSDWED-IGLLQRRLEHLAYNGYDGYSFSSVRSVVPEPYLPVIATLEAVRLGADLRGSGGTREAVVQRLREGDPRKRRSFVRFSEAVSLHRKQQEESTLSRVSNLAASLLFPXXXXXXXXXXXXXLFGRDDELRRIFLAAIKSHEAYGAILLTEVDGGGCPQEQRNVGIGAAASAADLIIHVNPSRFADLVRRVVDVRLVDPRQQVKVEQAMAACPSVRPM----LLTLTDQHKRFVKAGEVSKDYLKFLWLRDMELGQASPEAPPLEMSEADVDVMVGSLLDVRFMYRVRDGHDAFVPDRYVVASCLPNKAGPEVDPGKMLELKAGSAVYSQKLKLVGAHAVPPGLVPRLLAWCGRGEGHIKACWKRGVCFAFKN----HMVLLYEVRDTAGN-SWIECHARGSMFDESARTALNDIGDQVNELINDRKYGFPGLGLIRGESEEALVSSDGDHEA---LVKRIVNMLRDQMNVKFMMDRSIKQDLAFL----RWPIPRLVCVLPAPESGAKPLEEKD----RSFDKWSATLRNWCLGGKRKGKGVATRKLRLFFLCPYDRSLAECGPGGQGYEVTELLTWAKKAIPLAKVGLALASTAFKVCTGLSIPEADFEATLGTKAGG 864          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A6H5L2L4_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2L4_9PHAE)

HSP 1 Score: 225 bits (573), Expect = 1.800e-56
Identity = 264/1013 (26.06%), Postives = 403/1013 (39.78%), Query Frame = 2

Query: 1286 NPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPA-----EKRTVGVEVTEWK-----------PREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWI-VEAATEKEDFSFPSVGAIVPPAWMDAIAAVEALGQERGNP-----FVMWDDALKSFQSHYKNK-----SNKPPGDIEPKSILIQALAHREAEGGILLSMED--------------RSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVH-RKLDEGNYEGVDLN---GCFKNMLDAMIRYLFIYRAPEPSNNGQEV-----YVVPARLPLYGKENVLAENI---GLGEAVEGIKAWFIRTH-LPSGIIGRFLAFTAESIVSSGACWQHGAHIKWKKDAHTYDVLLYETTENKGTRTWPAISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDIRGLTSNELQEHLIPYLDTQFSNLTKILENISNYSVRI---FKNSFPSYFDPEKQPYPRLVVLKPVAEDQEASTGEHDEGTIHSREMWNRWARLW--KSRRAGDS-----CYLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSAL------VQSAFGEFEEHLPRDFDADPTGFNVDATWEEGQS------GRFKELHEP----AYEKLCEFMKKEEGSKGACPCLHRFLCGKTSTQP-KNWRDEMEQVKHGN--GEWAWVRRSNLKSF 4075
            NPW  PP+ V+ +G+     + +D+ ++G    N+LKVVLVGL +AGKT++     GR  +     E+RTVGVE+ + K           P +EL   VSLWDFAGQ  YY +HQ+FLT GALF+LVVD+F              A+ +WL+IL  RVPG+++ LVGTH D F   A+ C+  + S   G                   SP    ++D   R       Y+ + AK+          RAE EL                                      G P   P ++         N+A   DW  ++    + ++   + GA +  +     A  + L +  GNP     FV + +A+  F +  +       SN    D EP+ I + A+   EA G I L+  D               ++ A   +IH++P    +LV+R++D  LV  R+Q ++ E + A    R         L   HK FI+ G + ++YL+FLW+    L E + E   L       + M+ +++   F+YR      +GQ       YVV + LP     +V    I     G A+   K   +R H +P G++ R L+                 H+          VLLYE     GT +W             A   L ++ +++  LI D+ + +PG+         GL  +   E  + Y D+    L + + ++    + +      S        + P PRLV + P  E       E D     S E W+   R W    +R G         + FLC YD S   CGP G+GY +    E  +  +P  +  L L  + +++   + +P  D  + L        S F E   H   +  A   G  +D    EG        G  +E   P     YE L E +K  E            + G     P  ++  +M  V  G    EWAWVR  N+  F
Sbjct:   66 NPWKEPPAKVMEKGMAHTAVFLKDLDDYGRAWYNRLKVVLVGLGEAGKTSIATRLEGRLGSSCPKPEERTVGVEIRDIKLGPGPTNEGIGPNDEL--DVSLWDFAGQRAYYDTHQMFLTPGALFVLVVDMFTYEVGQ----SKEDALEQWLEILQARVPGSIMLLVGTHTDLFKANAAECTRRTDSFKEGRS------YCSTSSNNSYLSPWPTDVDDIMTRM-----RYECDSAKD----------RAEAELGE-----------------------------GHTTDLKGNPRYQPLRVVLEEDLLALNLAS-SDWQDIDLLQHRLEYLAYNGGADLRGSGSTREAVAQRLRE--GNPKKRRSFVRFSEAVSLFAAQQEGSMLSWLSNSFLRDNEPRRIFLAAVKSHEAHGAIHLTGVDGGGGSQEQHNTGIGAAASAADLIIHVNPSRFADLVRRVVDVRLVAPRQQAKVEEAMEACPLVRS----SLLTLTDQHKRFIKTGEVSKDYLKFLWLRDMDLREASQEAPPLEMSEADVEVMVSSLLDVRFMYRV----RDGQHAFVPDRYVVASCLPNKAGPDVDPGKILELKTGNAIYSQKLKLVRVHAVPPGLVPRLLS----------------CHL----------VLLYEVRATDGT-SWIECHARGNVFDESARTALKDIGDQVDELISDHKNRFPGL---------GLVRSGESEETVVYSDSDHKALLERIVDVQRDQMNVKLMMDRSMRQDVAFLRWPIPRLVCVLPAPESGAKPLEEGD----RSFEQWSATLRNWCLGGKRTGKGFATRKLRMFFLCPYDRSIAECGPGGQGYEVTELVEWAKKAMPSAKVGLALASIALRVCIGLAIPEVDFKATLGTKAGGALSTFAEESLHSGVESVASIAGDRLDGGRAEGLPHGAGGHGAQQEWPSPMQGITYETLKEVIKSFE------------VDGMRHRSPFPSFDVKMRLVDRGGRGAEWAWVRTKNIDQF 959          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: D8LLG1_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLG1_ECTSI)

HSP 1 Score: 180 bits (456), Expect = 6.970e-42
Identity = 264/1075 (24.56%), Postives = 414/1075 (38.51%), Query Frame = 2

Query: 1265 LGLFLDGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQ-----PAEKRTVGVEVTEWKPREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEGRQRASAG---LRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKEDFSFPSVGAIVPPAWMDAIAAVEAL--------------------GQERGNPFVMWDDALKSFQSHYKNKSNKPPGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFSRLYKAHKSFIRQGRLDQEYLRFLWVHRKLDEGNYEGVDLNGCFKNMLDAMIRYLFIYRAPEPSNNGQ-EVYVVPARLPLYGKENVLAENIG-LGEAVE-GIKAWFI----RTHLPSGIIGRFL------------------------AFTA---ESIVSSGAC--------------------WQHGAHIKWK-KDA--HTYDVLLYETTEN----KGTRTWP-AISVFVKCERSKASDVLAELEEKLKSLIRDNVHGYPGVCLPAFDDI-RGLTSN--ELQEHLIPYLDTQFSNLTKILENISNYSV--------------RIFKNSFPSYFDPEKQPYPRLVVLKPVAEDQEASTGEHDEGTIHSREMWNR--WARLWKSRRAG-----DSCYLVFLCAYDFSEIPCGPEGKGYLIQNPGELVQSMLPLLQGALWLLRVGIKIVSAIDLPLNDLSSALVQSAFG---EF-EEHLPRDFDADPT--------GFNVDATWEEGQSGRFKE-LHEP------AYEKLCEFMKK---EEGSKGACPCLHRFLCGKTSTQPKNWRDEMEQVKHGNG--EWAWVRRSNLKSF 4075
            L + L GNPW  PP  V+  G D V  +   ++  G      LKVVLVG   AGKTT+ R     +     P   R + V +  W P  + PL V +WDFAG  +Y+S+HQ+FLT GAL LLVVD+ +  RD   +    G ++ WLD L  RVPG+ V +V TH D F                                                   D  RS  A  A +  V  +    R +   + R   ++     + AS G   +  +P     P    +   G  +S+ G+    S+D  + V E K W V      E   FPSV  IVP  W    A ++AL                    G+++ + FV  + A + + SH   +S             ++ ++ R  +  + L     S  A   +IHL+P W+  L++ ++DH L D  +     ++L AYE  RG      + L + H+ F   G L +E+LRFLW          +GVD    F  +++ M  +  ++   +    G    ++VPARLP    + V  ++   L +A+  G +  F+      ++P  II +FL                        AF A   E +V  G                      ++ G  IK   KD     Y  L ++++ +     GT  W  ++    K       D+  +L+++L+S++ + +  + G  L   + + R L S   + Q      LD +   +   +E +   S+              R   NS          P P L+ + P  E       E D     S E W+     R  +  + G         L FLC  D S   CGP+G+GY +       +   PL +  L L  + +K+ + + +P  D+ + L  +A G   EF EE L    +A  T        G   +     G     +E +  P      AY++L E ++    +E  KG  P               ++   M+ V  G    EWAWVRR N+  +
Sbjct:  146 LSVDLKGNPWETPPWNVIQGGWDQVVSFHEGLSQSGGQVVPSLKVVLVGAVGAGKTTLARGLLDGKIAQTLPPRTRGIDVHIQPWVPDFDPPLEVLIWDFAGHDDYHSTHQMFLTDGALHLLVVDLHKFDRD---VLSRGGGVYIWLDSLLCRVPGSAVLVVATHADAFGD-------------------------------------------------DHERSAGALEALKAAVTEHLETKRQDWLAAKRLEERELMSQARPASTGRDFMSESPADRSKPAPPSLHLCGF-ISVSGR----SLDGLSTVGE-KIWEVA----NEPAVFPSVRKIVPGIWRRVWAVMDALHVGADPDSAVRLEGPVVTIEGRDK-HEFVTEEQAFEVW-SHADRESATQLEAAGHFGEQLRLVSSRHFKAALNLRHMGGSVLAVCGLIHLNPSWINVLLREVLDHRLTDPNEDEWWWKQLKAYERNRGIP---INELLETHRHFTTTGILTEEFLRFLW------RDVLKGVD-EKVFARLVETMTAHDAMFPCNDGVTEGNVREFMVPARLP----DGVTGDSFAKLEDAMSRGTRMQFVIEIYAKYVPPAIIAKFLGGFGRDESGVFRNLMFRTCWARGVAFVADGRECLVRVGEVSARPLPMIEINIAGPDVNDVFKVGFEIKKNVKDLLREAYPGLAFDSSAHPRFENGTEAWQDSLEALQK-------DLFDKLDQRLESVLSEFLEAFKGQSLSVNEAVARRLNSLCLDCQRDANFQLDAKVHEVVARVEQVVRASLSTTAVPKGGLNETERGILNSLARNVAFLLWPIPSLMCVLPARESGAKPLDERD----RSFEEWSTVLQKRCSEGNKMGRGVATQKLRLFFLCPLDMSLAECGPDGQGYQVTELLSWAKKAKPLAELGLALASIAVKVCTGLAMPAADIEAVLGTNAGGVLSEFVEEALTSSVEAMATVAGERLEDGSPAERVHHAGACRLEREKMRRPTPVQGFAYDQLKEVIRSFDVDETQKGKSPV-------------PSFDKTMQLVDRGGRGVEWAWVRRRNVDQY 1118          

BLAST of mRNA_H-canaliculatus_F_contig1299.2473.1 vs. uniprot
Match: A0A8J9VRT8_BRALA (MFHAS1 protein n=1 Tax=Branchiostoma lanceolatum TaxID=7740 RepID=A0A8J9VRT8_BRALA)

HSP 1 Score: 118 bits (295), Expect = 1.180e-22
Identity = 136/517 (26.31%), Postives = 215/517 (41.59%), Query Frame = 2

Query: 1277 LDGNPWVRPPSTVLSQGLDAVEKWWRDIANFGEGTSNKLKVVLVGLAKAGKTTVVRHFTGRQPA----EKRTVGVEVTEWKPREELPLSVSLWDFAGQSEYYSSHQIFLTQGALFLLVVDVFELSRDPPGLADPRGAIFRWLDILHDRVPGAVVGLVGTHGDKFFHQASPCSSSSSSSSCGLDDSXXXXXXXXXXXXXXXSPGQQQLEDAEKRFLDLVRSYQAERAKEDKVLRNYAQSRAEGELSSRKASKDAEEG--RQRASAGLRRTPGSFRMPPARVMKTTGKPLSIPGKIFRTSVDRRNNVAELKDWIVEAATEKEDFSFPSVGAIVPPAWMDAIAAVEAL---GQERGNPFVM---WDDALKSFQSHYKNKSNKP--------------PGDIEPKSILIQALAHREAEGGILLSMEDRSSPAEGDVIHLDPRWLMELVKRIIDHNLVDERKQGRIPEELVAYEETRGPTPGGFS--RLYKAHKSFIRQGRLDQEYLRFLWVHRKLDEGNYE 2743
            +DGNP ++PP+ V S+G+DA+ +++ ++    E  S +LKVV++G   AGKT++VR            E RT  VE+T+W P + +   V  +DF G   Y+ +H+ FLTQGAL LL V++             R AI  WLD L+ RVPGAVV +VG+  D        CS +                                  D E++  D+   ++ +     + +R         +L + +  KD + G  ++     L RT         R + T  +PL + G    +S +  + +  LK  IVE+A   +   FP +  I+P  W      +  L   G E GN  V    W  +  SF       S KP               G IE +  L   L++ +  G IL   +    P   D +  DP  L++++K +  H             +LV     + P   GFS  +L K  ++ + +G L +E +  L        GN +
Sbjct:  603 VDGNPLIKPPAEVCSEGIDAIRQYFEELERSDEKVSARLKVVVLGEKMAGKTSLVRTLVSGVSTLTEEEDRTHCVEITQWTPDDNITFEV--YDFGGHDVYHLTHRFFLTQGALNLLTVNLKTYRCTEQSY---REAIGFWLDTLNARVPGAVVTIVGSKTDM-------CSDA----------------------------------DIEEKTRDIQERFKQQNDVWQETIRQQIT-----KLENARTVKDVKVGTDQEEVQQQLERT---------RQLLT--RPLRLTGVFCVSSAESTSGLDTLKSHIVESANNTQ--LFPILRIILPQTWAKFEKRLRNLRDRGTETGNSDVSTQSWYFSDYSFSLDSDLDSGKPVWLTRDECLQHGKRAGLIEDR--LEPVLSYLQQVGTILRYTD---IPELKDFVFHDPSGLIDVIKELFHH-------------DLVKVFTNKNPRLKGFSNTKLKKIRRNLLGRGFLPREVVSALLGPHATPVGNVD 1037