prot_H-canaliculatus_F_contig1287.2391.1 (polypeptide) Hapterophycus canaliculatus Oshoro5f female

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: A0A6H5JR39_9PHAE (ATP-dependent DNA helicase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JR39_9PHAE)

HSP 1 Score: 470 bits (1210), Expect = 5.350e-147
Identity = 275/493 (55.78%), Postives = 355/493 (72.01%), Query Frame = 0

Query:    5 LGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINE---GGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASA-----------------TMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFYASLEEVGA 477
            L +EQ+ V+DLVLKGKSVF TGAAGTGKS+L+KKLHQ L  A +G+ TF+TGTTG AAVGIGGCT+HSFAGIG G++  E+LV R +++  A++ WA C VLVIDEISMM A+LFDKL++VGQR+KGN  PFGG+QLVLCGDFFQLPPVG+ +G  R+CFE  TWD ++ +SIVLK+VFRQ DP F++IL+++REGR++ + +  L  +V      R  +IN    GG S        XXXXXXXXXXXXXXXXXXXXXXX                      + T++FSRN+   R N EEL+ +QG +E Y + D+G    L  ++   A  + +ELKVGA+V+LL+NL++S+ LVNGATGVV EFV+   G RLP V+FD +EG    G VV V+ +  +++S+G+RE+A R+Q+PLRLAWA++VHKSQG T+A+ MVS   MF+FGQAYVALSR TTL GLHL++F    +KAHESV  FYA LE   A
Sbjct:  176 LCEEQRHVIDLVLKGKSVFFTGAAGTGKSFLLKKLHQALRQADQGATTFMTGTTGKAAVGIGGCTIHSFAGIGLGKDSVEQLVGRVKRSRNAQKRWAQCKVLVIDEISMMEADLFDKLNLVGQRVKGNGQPFGGIQLVLCGDFFQLPPVGIGRGSTRFCFEARTWDTSIDQSIVLKQVFRQKDPFFLKILHEMREGRVSSEAEQVLADKVEQCRLERYQQINAKSTGGISAAAPAESGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSYTRLFSRNEDVDRLNVEELQKLQGRAEFYEAEDNGDQMYLTQLQKHCAAGQTIELKVGAKVLLLKNLDSSSQLVNGATGVVKEFVEAS-GRRLPMVEFDTIEG---GGKVVSVISEEEWTVSLGDREMARRVQLPLRLAWALSVHKSQGMTIAHVMVSTRGMFEFGQAYVALSRATTLDGLHLVEFQKGVVKAHESVKKFYAGLERRAA 664          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI000711CEED (hypothetical protein n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI000711CEED)

HSP 1 Score: 298 bits (763), Expect = 4.240e-85
Identity = 171/466 (36.70%), Postives = 258/466 (55.36%), Query Frame = 0

Query:    5 LGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFV-DMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            L  EQ+ V    L+G ++F TG AGTGKSY+++ +   L +    +  ++T +TGIAA  I G T+HSFAGIG G E   + V+R  QN  A+Q W  C VL+IDEISM+   LF+KL  + +R++G+ L FGG+Q+++CGDFFQLPPVG+ +    YCFE++ W+A + ++I++K+VFRQ D +   +L DIR G ++   Q++++   R + +                                           T  +   TK+F+ N +    N+  L +IQ  S  Y + D G S  L  ++        L LK GA+VMLL+NL+ S GLVNG+ GVV  +  D   G  +P V+F        +G ++R V    F+M +G+R VA R+Q+PL+LAW I++HKSQG T+    V +  +F+ G  YVALSR   L  + L +F+   +  ++ V  FY
Sbjct:   69 LSDEQREVYMSALEGYNLFFTGDAGTGKSYVLRLIVSALKEKYGANNIYVTASTGIAACNISGTTLHSFAGIGVGDETVTKCVRRILQNKKAKQRWQTCEVLIIDEISMLDGRLFEKLEAIARRVRGSDLCFGGIQVIVCGDFFQLPPVGLGQNNVVYCFESECWNAVIQKTIIMKKVFRQKDAQLQALLRDIRYGHVS---QSSIQMISRMSRND-----------------------------------------LTHNAVMPTKLFAYNKSVDEYNESALNHIQSDSIEYKAVDDGDSNYLIQLQKSCQAPAVLSLKPGAQVMLLKNLSVSEGLVNGSRGVVDSYTKDPESGQMIPVVRF-----LTSTGDILRTVTHKEFTMELGSRVVARRIQLPLKLAWGISIHKSQGMTIDLLDVDLRGVFEAGMTYVALSRGVALNRMCLRNFSIRGVITNKKVIRFY 485          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI001EAF6C03 (ATP-dependent DNA helicase PIF1 n=2 Tax=Osmia TaxID=124287 RepID=UPI001EAF6C03)

HSP 1 Score: 297 bits (760), Expect = 6.570e-85
Identity = 176/466 (37.77%), Postives = 253/466 (54.29%), Query Frame = 0

Query:    4 ELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            +L +EQKR+VD VL GK+VF TG AGTGKS+L+KK+ + L        T  T +TG+AA  IGG T+H FAGIG G    +R  Q A ++  +   W     L++DEISM+ A+ FDK+  V + ++ N  PFGG+QL+LCGDFFQLPP+       ++CF++++W+  VH +  L+ V RQ DP F++ILN+IR GR+T    N L+   +      G                                               T + S        N+ +L  ++G S++Y++ DS  S ++  +     V  KL LKVGA+VMLL+N+N S GLVNGA GVV EFVD      +P V+F      H        +++ T  M+ G+  +  R Q+PL+LAWA ++HKSQG TL    + +  +F+ GQ+YVALSR  +L  L +LDF +  + AH  V  FY
Sbjct:  178 QLTEEQKRIVDAVLSGKNVFFTGGAGTGKSFLLKKIIEALPP----DVTIATASTGVAACHIGGVTLHQFAGIGLGTANLDRCYQMAARSSASI--WRKIKYLILDEISMIDADYFDKIEAVARHIRKNERPFGGIQLILCGDFFQLPPISKLDKQTKFCFQSESWEKCVHFNFELQTVHRQKDPEFIKILNNIRIGRVTDSITNTLKATAKQKIEGNG--------------------------------------------ILATTLCSHVKEAEEINEFQLNELKGESKVYVAQDSDQS-MVTTLNKQLTVPNKLILKVGAQVMLLKNINVSKGLVNGARGVVTEFVD-----NMPVVKFKSGIQYHAK------IEKWTLKMNSGS--IIFRKQVPLKLAWAFSIHKSQGLTLDCVEMCLARVFNAGQSYVALSRAQSLQSLRVLDFESQQVWAHPDVLVFY 579          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI000258E8D1 (ATP-dependent DNA helicase PIF1 n=1 Tax=Megachile rotundata TaxID=143995 RepID=UPI000258E8D1)

HSP 1 Score: 296 bits (759), Expect = 8.850e-85
Identity = 178/467 (38.12%), Postives = 246/467 (52.68%), Query Frame = 0

Query:    3 DELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            ++L +EQ+R++D VL GK+VF TG AGTGKS+L+KK+   L        T  T +TG+AA  IGG T+H FAGIG G    +R  Q A +N      W     L++DEISM+ A+ FDK+  V + ++ N  PFGG+QL+LCGDFFQLPP+  K    ++CF+++ WD  VH +  L+ V RQ DP FV ILN+IR GRIT      L+   +      G                                               T + S  +     N+ +L  ++G S++YI+ DS  S +   +     V  KL LK+GA+VMLL+N+N S GLVNGA GVV+EFVD      +P VQF           +        +S+      V  R QIPL+LAWA ++HKSQG TL    + +  +F+ GQ+YVALSR  +L  L +LDF +  + AH  V  FY
Sbjct:  176 EQLTEEQQRILDAVLSGKNVFFTGGAGTGKSFLLKKIIGALPP----DVTMATASTGVAACHIGGITLHQFAGIGLGTANLDRCYQMASRNSATM--WRKTKYLIMDEISMIDADYFDKIEAVARHLRKNERPFGGIQLILCGDFFQLPPISTKDRKAKFCFQSNVWDKCVHLNFELRTVHRQKDPEFVSILNNIRIGRITDKITETLKATAKQKIEDNG--------------------------------------------ILATTLCSHVNEAEEINEFQLNELKGESKVYIAQDSDQS-MTTTLNKQLTVPDKLVLKIGAQVMLLKNINVSNGLVNGARGVVLEFVD-----NMPLVQF--------KSSIQYRAKIEKWSLKTNAGSVIVRKQIPLKLAWAFSIHKSQGLTLDCVEMCLARVFNAGQSYVALSRAQSLQSLRVLDFESQQVWAHPDVLVFY 578          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: A0A7S1CHL2_9STRA (ATP-dependent DNA helicase (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CHL2_9STRA)

HSP 1 Score: 288 bits (738), Expect = 6.320e-84
Identity = 172/457 (37.64%), Postives = 248/457 (54.27%), Query Frame = 0

Query:   28 AGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKK----GMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFV------DMRWGPRLPKVQFDPVEGCHDSGPVVR-VVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFYASLE 473
            AGTGKS+L+++L   L DA      F+T +TGIAA  IGG TVHSF+GIG G+E  + L  R  +N  A + W    VL++DEISM+  +L DKL  V +R++ N  PFGGVQL++CGDF QLPPVG+      G  ++CF+ + W+  V    VL +VFRQ D RFV +LN++R G++T +    L+     AA S                                           S     T++FS N   +  N+  L  + GP   Y + DSG     +N++ +     +L LK GA+VMLL+NL+  AGLVNG+ GVV++F       D   G     ++F  V+   DSG + R ++    +++  G    ASR Q+PL+LAWA+++HKSQG T+    V++  +F+ GQAYVALSR T+L  L +  F  + ++AH  V  FY  L+
Sbjct:    1 AGTGKSFLLRRLVLSLRDAKGEESVFVTASTGIAACNIGGLTVHSFSGIGLGKEDRKFLGDRVVRNRNATKRWRAAHVLIVDEISMLDGDLLDKLEYVARRVRNNEAPFGGVQLIMCGDFHQLPPVGMSDRGGGGRVKFCFQAECWERLVQSEFVLDQVFRQKDNRFVTLLNELRHGKVTAETIAHLQ-----AAGS-----------------------------------------GLSDDMVPTRLFSTNSDVSAINRRHLAELTGPEHTYDAVDSGLDPYRSNLQKNCQAAARLSLKPGAQVMLLKNLDVKAGLVNGSRGVVVDFQEDTELDDDEVGVGGRGIKFPIVDFFSDSGDMKRRMIRPEEWTIEEGASIKASRRQVPLKLAWALSIHKSQGMTIDALTVNLSRVFECGQAYVALSRATSLERLRVEGFDPARVRAHPDVVRFYRRLK 411          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI00083C38E9 (ATP-dependent DNA helicase PIF1 n=1 Tax=Eufriesea mexicana TaxID=516756 RepID=UPI00083C38E9)

HSP 1 Score: 295 bits (754), Expect = 7.010e-84
Identity = 181/468 (38.68%), Postives = 256/468 (54.70%), Query Frame = 0

Query:    3 DELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRV-VDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            +++  EQ ++++ VL GK+VF TG+AGTGKS+L++ +   L        T  T +TG+AA  IGG T+H F+GIG G    ER VQ A +N  A  +W     L+IDEISM+  + FDK+  V + ++ +  PFGG+QL+LCGDFFQLPPV  K    ++CF+++ W+  VH +  L+ V RQ DP FV+ILN IR GR+T D    L+   +    + G                                               T++ S  +     N+ +L +++G S+IYI+ DS  S + N +     V  KL LKVGA+VMLL+N+N S GLVNGA GVV +FVD      +P VQF        SG   R  +++     ++G   V  R QIPL+LAWA ++HKSQG TL    +S+  +F+ GQAYVALSR  +L  L +LDF +  + AH  V  FY
Sbjct:  193 EQITDEQNKILNAVLSGKNVFFTGSAGTGKSFLLRTIIAALPP----DVTVATASTGVAACHIGGQTLHQFSGIGLGTANLERCVQMASRNSSAA-SWRKTKHLIIDEISMVDGDYFDKIEAVARHIRKSERPFGGIQLILCGDFFQLPPVSTKDKNTKFCFQSEAWEKCVHFNFELQTVHRQKDPEFVKILNSIRIGRVTDDIAERLKATAKQKIENNG--------------------------------------------ILATRLCSHVNEAEEINEHQLNSLEGESKIYIAQDSDVS-MTNTLNQQLTVPDKLILKVGAQVMLLKNVNISDGLVNGARGVVTKFVD-----NVPIVQFR-------SGLQYRAKLEKWNLKTNLGT--VVHRKQIPLKLAWAFSIHKSQGLTLDCIEMSLARVFNAGQAYVALSRAQSLQSLRVLDFHSQQVWAHTDVLMFY 596          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: A0A7M7R825_APIME (ATP-dependent DNA helicase PIF1 n=6 Tax=Apis TaxID=7459 RepID=A0A7M7R825_APIME)

HSP 1 Score: 292 bits (747), Expect = 6.860e-83
Identity = 175/469 (37.31%), Postives = 254/469 (54.16%), Query Frame = 0

Query:    3 DELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGS--KLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            +++ +EQ R++D VL+GK++F TG+AGTGKS+L++K+   L        T  T +TG+AA  IGG T+H FAGIG G   +ER  + A ++  A   W     L+IDEISM+  + FDK+  V + ++ N  PFGG+QL+LCGDFFQLPPV  K    ++CF+++ W   +H +  L+ V+RQ DP F++ILN+IR GR+T D    L+   +    + G                                               T++ S  +     N+ +L  ++G S+ YI+ DS  S  K+LN       V  KL LK+GA+VMLL+N+N S GLVNGA GVVI+F D      +P VQF      H        +++     ++G   +  R QIPL+LAWA ++HKSQG TL    + +  +FD GQ+YVALSR  +L  L +L+F    + AH  V  FY
Sbjct:  194 EQVSEEQNRILDAVLRGKNIFFTGSAGTGKSFLLRKIIAALPP----DVTIATASTGVAACHIGGITLHQFAGIGLGTANSERCFEMASRSSSATL-WRKTKHLIIDEISMVDGDYFDKIEAVARHIRKNERPFGGIQLILCGDFFQLPPVSKKDKKAKFCFQSNAWTKCIHFNYELQTVYRQKDPEFIKILNNIRIGRVTDDIAKTLKATAKQKIENNG--------------------------------------------ILATRLCSHVNEVEEINEFQLNELKGESKTYIAQDSDQSMTKILNQ---QLTVPDKLILKIGAQVMLLKNINVSNGLVNGARGVVIKFAD-----NVPIVQFKSGIQYHAK------LEKWNLKTNIGT--IVCRKQIPLKLAWAFSIHKSQGLTLDCVEMCLARVFDAGQSYVALSRAQSLQSLRVLEFNNQQVWAHSDVLMFY 597          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI0005ABD807 (ATP-dependent DNA helicase PIF1 n=2 Tax=Opiinae TaxID=68883 RepID=UPI0005ABD807)

HSP 1 Score: 291 bits (746), Expect = 1.270e-82
Identity = 181/466 (38.84%), Postives = 251/466 (53.86%), Query Frame = 0

Query:    4 ELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFY 469
            EL  EQK V+D VLKGK++F TG+AGTGKS+L+K++   L  A     T  T +TG+AA  IGG T+H F+GIG G    ER  Q A +   A   W     L+IDEISM+  E F+K+  V + ++ N  PFGG+QL+LCGDFFQLPPV       ++CF++  W+  VH +  L+ V RQ DP+FV++LN +R GR+T +    L+       S+   +I +GG                                        T++ S  +     N+ +L N++G ++ Y++ DS  S +   +     V  KL LKVGA+VMLL+N+N S GLVNGA GVVI+F D       P VQF   E  H             +S+   +     R Q+PL+LAWA ++HKSQG TL    +S+  +FD GQAYVALSR  +L  L +LDF    + A+ SV  FY
Sbjct:  199 ELTDEQKGVLDAVLKGKNIFFTGSAGTGKSFLLKRIIAELPPAV----TTATASTGVAACHIGGITLHQFSGIGLGTGTVERCYQMASRPATAT-IWRRTKYLIIDEISMVDGEYFEKIEAVARHVRKNDRPFGGIQLILCGDFFQLPPVNKTSDTKKFCFQSKAWEKCVHLNFELQRVHRQKDPKFVKVLNSLRIGRVTDEITTILK-------STSSQKIEKGG-------------------------------------ILATRLCSHVNEANEINESQLANLKGSTKTYVAQDSEES-MTKTLDQQLPVPGKLTLKVGAQVMLLKNINISNGLVNGARGVVIKFED-----ETPVVQFRSGEIYH--------ARFEKWSIKTASGAFVHRKQVPLKLAWAFSIHKSQGLTLDCVEMSLAKVFDAGQAYVALSRAQSLDSLRVLDFNGRQVWANTSVLEFY 601          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: UPI0015891689 (ATP-dependent DNA helicase PIF1 n=1 Tax=Chelonus insularis TaxID=460826 RepID=UPI0015891689)

HSP 1 Score: 291 bits (745), Expect = 1.370e-82
Identity = 177/468 (37.82%), Postives = 260/468 (55.56%), Query Frame = 0

Query:    5 LGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFYASL 472
            L +EQ  V++ VL+GK++F TG+AGTGKSYL++++   L        T +T +TG+AA  IGG T+H FAGIG G    E   + A + + A   W     L+IDEISM+ ++LFDK+  V + ++ +  PFGG+QL+LCGDFFQLPPV       ++CFE+++W+  VH +  L+ V RQ DP FV++LN+IR GR+T + +N L+   + +    G                                               T++ S        N+ +L N++G S+IYI+ DS  S +   +    +V  KL LKVGA+VMLL+N+N + GLVNGA GVV +F +      +P VQF     C  SG V  V  +  +++ + N  + SR QIPL+LAWA ++HKSQG TL    +++  +FD GQAYVALSR  +L  L ++DF    +  + +V  FY  L
Sbjct:  197 LTEEQNEVLEAVLRGKNIFFTGSAGTGKSYLLRRIIGELPP----DVTTITASTGVAACHIGGITLHQFAGIGLGNSNLECCYKLASRPNIAA-CWRKTKYLIIDEISMVDSQLFDKIEAVARHIRKSDKPFGGIQLILCGDFFQLPPVKKNDEKSKFCFESESWEKCVHYNFELRTVHRQKDPTFVQLLNNIRIGRVTDNIKNILKATAKQSIEKDG--------------------------------------------ILATRLCSHVREVDEINESQLNNLEGISKIYIAQDSDDS-MTKTLNQQLSVPGKLILKVGAQVMLLKNININDGLVNGARGVVTKFEN-----DIPVVQF-----C--SGKVYHVKSEK-WTVRIANGNLISRKQIPLKLAWAFSIHKSQGLTLDCVEMNLSKVFDAGQAYVALSRAQSLETLRVIDFNEDQVWVNTNVLQFYKKL 601          

BLAST of mRNA_H-canaliculatus_F_contig1287.2391.1 vs. uniprot
Match: A0A8C2VKX3_CHILA (ATP-dependent DNA helicase PIF1 n=2 Tax=Chinchilla lanigera TaxID=34839 RepID=A0A8C2VKX3_CHILA)

HSP 1 Score: 288 bits (737), Expect = 1.510e-82
Identity = 185/479 (38.62%), Postives = 260/479 (54.28%), Query Frame = 0

Query:    4 ELGQEQKRVVDLVLKGKSVFVTGAAGTGKSYLIKKLHQVLTDAGEGSGTFLTGTTGIAAVGIGGCTVHSFAGIGQGREPAERLVQRAQQNDFARQNWADCSVLVIDEISMMSAELFDKLSVVGQRMKGNSLPFGGVQLVLCGDFFQLPPVGVKKGMPRYCFETDTWDAAVHESIVLKEVFRQMDPRFVRILNDIREGRITLDGQNALEGQVRPAASSRGGRINEGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSASATMTKIFSRNDACARCNKEELENIQGPSEIYISSDSGGSKLLNNVKMDSAVEKKLELKVGARVMLLRNLNTSAGLVNGATGVVIEFVDMRWGPRLPKVQFDPVEGCHDSGPVVRVVDQATFSMSVGNREVASRLQIPLRLAWAITVHKSQGATLANAMVSMEDMFDFGQAYVALSRTTTLAGLHLLDFTASNIKAHESVTHFYASLEEVGAGVKSP 482
            +L +EQ  V+ LVLKG+SVF TG+AGTGKSYL+K++   L  AG    T  T +TG+AA  IGG T+H+FAGIG GR P  + V  AQ+    RQ W +C  LVIDEISM+ A+LFDKL  V + ++    PFGGVQL++CGDF QLPPV      PR+CF+  +W   V  ++ L EV+RQ DP FV +L  +R GR +    + +  Q+R  A+ + GR                                             T++ +  D     N+  L+ + G    + + DS   +L   +     V + L+LK+GA+VML++NL  S GLVNGA GVV+ F     G  LP+V+F           V  V+    +++     +V SR Q+PL+LAWA+++HKSQG +L    +S+  +F  GQAYVALSR  +L GL +LDF    +     V  FYA+L     G++SP
Sbjct:   91 QLSEEQAAVLGLVLKGQSVFFTGSAGTGKSYLLKRILGSLPPAG----TVATASTGVAACHIGGTTLHAFAGIGSGRAPLAQCVALAQRPGV-RQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQEKPFGGVQLIICGDFLQLPPVTKGSQPPRFCFQAKSWRRCVPVTLELTEVWRQADPAFVSLLQAVRLGRCS----DEVTRQLRATAAHKVGRDG----------------------------------------VVATRLCTHQDDVVLTNERRLQELPGEVHSFEALDSH-PELARALDAQCPVSRLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAE--GRGLPQVRF--------LCGVTEVIRTDRWTVQATGGQVLSRQQLPLQLAWALSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPLVVHCDARVLRFYATLRR-SLGLESP 508