mRNA_H-canaliculatus_F_contig12368.2017.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_H-canaliculatus_F_contig12368.2017.1
Unique Name	mRNA_H-canaliculatus_F_contig12368.2017.1
Type	mRNA
Organism	Hapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)

Homology

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A0A0VDF3_SACJA (Pyruvate, phosphate dikinase n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A0A0VDF3_SACJA)

HSP 1 Score: 216 bits (549), Expect = 4.480e-63
Identity = 103/111 (92.79%), Postives = 107/111 (96.40%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +VI+EIKKKKG+KLDLEMEA DWKEVV  FKKLNPNVPTDPREQLRGA+LAVFRSWNNDRAIRYRSYNGIPD WGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG
Sbjct:  247 EVITEIKKKKGVKLDLEMEAHDWKEVVDRFKKLNPNVPTDPREQLRGAVLAVFRSWNNDRAIRYRSYNGIPDEWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 357

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A097IU19_9PHAE (Pyruvate, phosphate dikinase n=1 Tax=Desmarestia viridis TaxID=62313 RepID=A0A097IU19_9PHAE)

HSP 1 Score: 213 bits (542), Expect = 3.510e-62
Identity = 100/111 (90.09%), Postives = 106/111 (95.50%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +V+  IK +KG+KLDLEM+A DWKEVVGEFKKLNPNVPTDPREQLRGA+LAVFRSWNNDRAIRYRSYNGIPD WGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG
Sbjct:  220 EVLDRIKARKGVKLDLEMDAEDWKEVVGEFKKLNPNVPTDPREQLRGAVLAVFRSWNNDRAIRYRSYNGIPDEWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 330

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A097IU13_9PHAE (Pyruvate, phosphate dikinase n=1 Tax=Ishige okamurae TaxID=233772 RepID=A0A097IU13_9PHAE)

HSP 1 Score: 213 bits (542), Expect = 3.930e-62
Identity = 102/111 (91.89%), Postives = 108/111 (97.30%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +VI+E+KKKKGIKLD+EM+A+DWKEVV EFKKLNPNVP DPREQLRGAILAVFRSWNNDRAIRYRSYNGIPD WGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG
Sbjct:  233 EVITEMKKKKGIKLDVEMQAADWKEVVEEFKKLNPNVPDDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDEWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 343

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A097IU36_9PHAE (Pyruvate, phosphate dikinase n=6 Tax=Sargassum TaxID=3015 RepID=A0A097IU36_9PHAE)

HSP 1 Score: 209 bits (532), Expect = 7.680e-61
Identity = 100/111 (90.09%), Postives = 106/111 (95.50%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +VI+ IKK+KGIKLDLEMEA+DWKEV  EFKKLNPNVP+DPREQLRGAILAVFRSWNNDRAIRYRSYN IPD WGTAV+VQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG
Sbjct:  203 EVITSIKKRKGIKLDLEMEAADWKEVAEEFKKLNPNVPSDPREQLRGAILAVFRSWNNDRAIRYRSYNNIPDEWGTAVNVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 313

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A097IU01_9PHAE (Pyruvate, phosphate dikinase n=1 Tax=Dictyopteris undulata TaxID=156997 RepID=A0A097IU01_9PHAE)

HSP 1 Score: 207 bits (527), Expect = 3.850e-60
Identity = 97/111 (87.39%), Postives = 108/111 (97.30%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +VI+EIKKKKG+KLDLE++ASDWK+VV EFKK+NPNVP DPREQLRGA+LAVF+SWNNDRAIRYRSYNGIPD+WGTAVSVQAMAYGNMND CGTGVAFSRNPSTGE+KFFG
Sbjct:  198 EVITEIKKKKGVKLDLELDASDWKQVVEEFKKINPNVPQDPREQLRGAVLAVFKSWNNDRAIRYRSYNGIPDSWGTAVSVQAMAYGNMNDECGTGVAFSRNPSTGEDKFFG 308

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A6H5L2G0_9PHAE (Pyruvate, phosphate dikinase n=3 Tax=Ectocarpales TaxID=2877 RepID=A0A6H5L2G0_9PHAE)

HSP 1 Score: 187 bits (476), Expect = 5.110e-53
Identity = 94/111 (84.68%), Postives = 97/111 (87.39%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +VI +IKKKKGIKLDLEMEASDWK+VV EFKKLNPNVP DPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAM           GVAFSRNPSTGENKFFG
Sbjct:  245 EVIDKIKKKKGIKLDLEMEASDWKQVVAEFKKLNPNVPMDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMXXXXXXXXXXXGVAFSRNPSTGENKFFG 355

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: V2YAA4_9FIRM (Pyruvate, phosphate dikinase n=4 Tax=Bacteria TaxID=2 RepID=V2YAA4_9FIRM)

HSP 1 Score: 143 bits (360), Expect = 2.590e-37
Identity = 69/116 (59.48%), Postives = 90/116 (77.59%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFK-----KLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            Q+I ++K+KKG+  D+E++A+D KE+ G+FK     K+  + P+DP+EQL GAI AVFRSWNN RAI YR  N IP +WGTAV+VQ+MA+GNM D CGTGVAF+RNP+TGE K  G
Sbjct:  155 QLIDKMKEKKGVTQDVELDANDLKELAGQFKAEYKEKIGADFPSDPKEQLMGAIKAVFRSWNNPRAIVYRRMNDIPGSWGTAVNVQSMAFGNMGDDCGTGVAFTRNPATGEKKLMG 270

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A7C4Y918_9BACT (Pyruvate, phosphate dikinase n=1 Tax=candidate division WOR-3 bacterium TaxID=2052148 RepID=A0A7C4Y918_9BACT)

HSP 1 Score: 142 bits (357), Expect = 6.640e-37
Identity = 68/116 (58.62%), Postives = 89/116 (76.72%), Query Frame = 1

Query:    1 QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNV-----PTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            ++I ++K++KG+KLD+E++A DWK VV  +KKL  +V     P DP+EQL GAI AVF+SWNN RAI YR  + IPD WGTA +VQAM +GN+ +  GTGVAF+RNPSTGEN F+G
Sbjct:  156 KIIEKVKEEKGVKLDIELDAEDWKRVVENYKKLVKDVTKKDFPQDPKEQLWGAIEAVFKSWNNKRAIEYRKIHKIPDDWGTACNVQAMVFGNLGENSGTGVAFTRNPSTGENVFYG 271

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: A0A847VSF9_9FIRM (Pyruvate, phosphate dikinase n=2 Tax=Erysipelotrichia bacterium TaxID=2184014 RepID=A0A847VSF9_9FIRM)

HSP 1 Score: 141 bits (356), Expect = 9.010e-37
Identity = 68/115 (59.13%), Postives = 87/115 (75.65%), Query Frame = 1

Query:    4 VISEIKKKKGIKLDLEMEASDWKEVVGEFK-----KLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +I EIK++KG+KLD E+ A+D KE+V +FK      +  + P DP+ QL  A++AVFRSWNNDRAI YR  N IP +WGTAV++Q M +GNM D CGTGVAF+RNP+TGENK FG
Sbjct:  156 IIDEIKEEKGVKLDTELCANDMKEMVAKFKAYYKESVGEDFPQDPKVQLMKAVIAVFRSWNNDRAIYYRRMNDIPSSWGTAVNIQMMVFGNMGDDCGTGVAFTRNPATGENKLFG 270

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BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Match: UPI000590B25B (pyruvate, phosphate dikinase n=1 Tax=Candidatus Soleaferrea massiliensis TaxID=1470354 RepID=UPI000590B25B)

HSP 1 Score: 140 bits (353), Expect = 2.290e-36
Identity = 69/115 (60.00%), Postives = 86/115 (74.78%), Query Frame = 1

Query:    4 VISEIKKKKGIKLDLEMEASDWKEVVGEFK-----KLNPNVPTDPREQLRGAILAVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSRNPSTGENKFFG 333
            +I E+K+KKG+KLD E++A D KE+V  FK     +LN   P+ P +QL GA+ AVFRSWNN RAI YR  N IP +WGTAV+VQAM +GNM D  GTGVAF+R+P+TGENK FG
Sbjct:  156 IIDEVKEKKGVKLDTELDAEDLKELVVRFKNYYKEQLNSEFPSTPHDQLMGAVKAVFRSWNNPRAIYYRRMNDIPSSWGTAVNVQAMVFGNMGDDSGTGVAFTRDPATGENKLFG 270

The following BLAST results are available for this feature:

BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot

BLAST of mRNA_H-canaliculatus_F_contig12368.2017.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A0A0VDF3_SACJA	4.480e-63	92.79	Pyruvate, phosphate dikinase n=1 Tax=Saccharina ja...	[more]
A0A097IU19_9PHAE	3.510e-62	90.09	Pyruvate, phosphate dikinase n=1 Tax=Desmarestia v...	[more]
A0A097IU13_9PHAE	3.930e-62	91.89	Pyruvate, phosphate dikinase n=1 Tax=Ishige okamur...	[more]
A0A097IU36_9PHAE	7.680e-61	90.09	Pyruvate, phosphate dikinase n=6 Tax=Sargassum Tax...	[more]
A0A097IU01_9PHAE	3.850e-60	87.39	Pyruvate, phosphate dikinase n=1 Tax=Dictyopteris ...	[more]
A0A6H5L2G0_9PHAE	5.110e-53	84.68	Pyruvate, phosphate dikinase n=3 Tax=Ectocarpales ...	[more]
V2YAA4_9FIRM	2.590e-37	59.48	Pyruvate, phosphate dikinase n=4 Tax=Bacteria TaxI...	[more]
A0A7C4Y918_9BACT	6.640e-37	58.62	Pyruvate, phosphate dikinase n=1 Tax=candidate div...	[more]
A0A847VSF9_9FIRM	9.010e-37	59.13	Pyruvate, phosphate dikinase n=2 Tax=Erysipelotric...	[more]
UPI000590B25B	2.290e-36	60.00	pyruvate, phosphate dikinase n=1 Tax=Candidatus So...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
H-canaliculatus_F_contig12368	contig	H-canaliculatus_F_contig12368:2273..3472 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90	2022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female	2021-02-24

Properties


Property Name	Value
Taxonomic scope	Eukaryota
Seed ortholog score	186.8
Seed ortholog evalue	5.6e-45
Seed eggNOG ortholog	2880.D8LI11
Preferred name	PPDK
KEGG rclass	RC00002,RC00015
KEGG ko	ko:K01006
KEGG Reaction	R00206
KEGG Pathway	ko00620,ko00710,ko00720,ko01100,ko01120,ko01200,map00620,map00710,map00720,map01100,map01120,map01200
KEGG Module	M00169,M00171,M00172,M00173
GOs	GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464
EggNOG free text desc.	pyruvate, phosphate dikinase activity
EggNOG OGs	2QREJ@2759,COG1080@1
EC	2.7.9.1
COG Functional cat.	G
BiGG Reaction	iRC1080.CRv4_Au5_s10_g148_t1
Best tax level	Eukaryota
Best eggNOG OG	NA\|NA\|NA
BRITE	ko00000,ko00001,ko00002,ko01000
Hectar predicted targeting category	other localisation
Ec32 ortholog description	Pyruvate, phosphate dikinase
Ec32 ortholog	Ec-18_001310.1
Exons	2
Model size	342
Cds size	342
Stop	1
Start	0

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622928951.9272883-CDS-H-canaliculatus_F_contig12368:2272..2347	1622928951.9272883-CDS-H-canaliculatus_F_contig12368:2272..2347	Hapterophycus canaliculatus Oshoro5f female	CDS	H-canaliculatus_F_contig12368 2273..2347 +
1696845626.8786917-CDS-H-canaliculatus_F_contig12368:2272..2347	1696845626.8786917-CDS-H-canaliculatus_F_contig12368:2272..2347	Hapterophycus canaliculatus Oshoro5f female	CDS	H-canaliculatus_F_contig12368 2273..2347 +
1622928951.9523604-CDS-H-canaliculatus_F_contig12368:3205..3472	1622928951.9523604-CDS-H-canaliculatus_F_contig12368:3205..3472	Hapterophycus canaliculatus Oshoro5f female	CDS	H-canaliculatus_F_contig12368 3206..3472 +
1696845626.8957555-CDS-H-canaliculatus_F_contig12368:3205..3472	1696845626.8957555-CDS-H-canaliculatus_F_contig12368:3205..3472	Hapterophycus canaliculatus Oshoro5f female	CDS	H-canaliculatus_F_contig12368 3206..3472 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_H-canaliculatus_F_contig12368.2017.1	prot_H-canaliculatus_F_contig12368.2017.1	Hapterophycus canaliculatus Oshoro5f female	polypeptide	H-canaliculatus_F_contig12368 2273..3472 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at H-canaliculatus_F_contig12368:2273..3472+
coding sequence from alignment at H-canaliculatus_F_contig12368:2273..3472+

protein sequence of mRNA_H-canaliculatus_F_contig12368.2017.1

>prot_H-canaliculatus_F_contig12368.2017.1 ID=prot_H-canaliculatus_F_contig12368.2017.1|Name=mRNA_H-canaliculatus_F_contig12368.2017.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=114bp
QVISEIKKKKGIKLDLEMEASDWKEVVGEFKKLNPNVPTDPREQLRGAIL
AVFRSWNNDRAIRYRSYNGIPDAWGTAVSVQAMAYGNMNDGCGTGVAFSR
NPSTGENKFFGGE*

mRNA from alignment at H-canaliculatus_F_contig12368:2273..3472+

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_H-canaliculatus_F_contig12368.2017.1 ID=mRNA_H-canaliculatus_F_contig12368.2017.1|Name=mRNA_H-canaliculatus_F_contig12368.2017.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=1200bp|location=Sequence derived from alignment at H-canaliculatus_F_contig12368:2273..3472+ (Hapterophycus canaliculatus Oshoro5f female)
CAGGTGATCTCTGAGATCAAGAAAAAGAAGGGGATCAAGCTCGACTTGGA
GATGGAGGCGTCGGATTGGAAGGAGGTAAGAGGACGACACACGGCGGAAG
CTGAAAAAACGGAAGTGGATGTTGTCCAGAAGAAGCTCTGTGGACAGCAT
GGCATGATCCCGCAAAGCTGTGAACGGCTTGAAAGAACTGCACGGGAAGA
TGTGTAGCGTTGAGTCGGGTTCTTCGGAAACTCTCAAGGATCTTTCGGCA
CCTTTTACAAAGCCCGCGGCTGGAAATGTGCCTGCTGGAAGGAGTCAGGG
AATACCTTAGGCACACCTATGTTTCCCTTTCCACATGGCACGTCAACGAC
GGCGTTCGCTCGGCATGACTGGAAGATCAGCAGCGAGAGGGGGCGGGTGG
TGGTACGCCTGCTGCTCGTGGTGAGGCGATAGCTCTGGGTCGTGCTGAAA
GCTGAAGGAATGGCCGGCGAACAGCAAAGCTAATAGGTTCCCTTGTCGGC
CACCACGAGCAGCATGCGCACCATGACCCGCCCCCTCTCGCTGCTGATCT
TCCGATCATGCCGAGCGAACGCCGTCGTTGACGTGCCGTGTCCCTCGCCA
CCCTCCCTCGCGCTCCCTCTAACGCCGGTTGCCGGAACAAGCAATCCTGT
CGTCCTTTTCCCGTGAACGAAGAAAAAAATTGAAGAAAAAAAAACGGTGC
TTGTGAACGAAAGAAAGAGGAAAAAGGAAGGTAAACGAAAGAGAAAAAAG
AAAAAGGTCAACGAAAGAAGAGAAAAAAATGGACGAAGACGAGCGCAGAC
GAGCGGGTTGGCCGGCCGGCCGGCCGGCCGGCCTATGCCTGGCCCACCCT
TCCCCTCTCCTTCGGTATCGACATTTGAAGAGGCAAAACGTTTTTTTTTC
GTGACTCTCTTTCGACAAAAAAAATTAAAAAAGGTCGTCGGCGAGTTCAA
GAAGCTCAACCCGAACGTCCCAACCGACCCGAGGGAGCAGCTCCGTGGCG
CCATCCTCGCGGTGTTCCGGTCCTGGAACAACGACCGCGCCATCCGCTAC
CGGTCGTACAACGGCATCCCTGATGCGTGGGGCACCGCCGTATCGGTGCA
GGCCATGGCCTACGGCAACATGAACGACGGCTGCGGTACGGGGGTGGCTT
TCTCGAGGAACCCTTCCACTGGAGAGAACAAGTTCTTTGGCGGTGAGTAA

Coding sequence (CDS) from alignment at H-canaliculatus_F_contig12368:2273..3472+

>mRNA_H-canaliculatus_F_contig12368.2017.1 ID=mRNA_H-canaliculatus_F_contig12368.2017.1|Name=mRNA_H-canaliculatus_F_contig12368.2017.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=684bp|location=Sequence derived from alignment at H-canaliculatus_F_contig12368:2273..3472+ (Hapterophycus canaliculatus Oshoro5f female)
CAGGTGATCTCTGAGATCAAGAAAAAGAAGGGGATCAAGCTCGACTTGGA
GATGGAGGCGTCGGATTGGAAGGAGCAGGTGATCTCTGAGATCAAGAAAA
AGAAGGGGATCAAGCTCGACTTGGAGATGGAGGCGTCGGATTGGAAGGAG
GTCGTCGGCGAGTTCAAGAAGCTCAACCCGAACGTCCCAACCGACCCGAG
GGAGCAGCTCCGTGGCGCCATCCTCGCGGTGTTCCGGTCCTGGAACAACG
ACCGCGCCATCCGCTACCGGTCGTACAACGGCATCCCTGATGCGTGGGGC
ACCGCCGTATCGGTGCAGGCCATGGCCTACGGCAACATGAACGACGGCTG
CGGTACGGGGGTGGCTTTCTCGAGGAACCCTTCCACTGGAGAGAACAAGT
TCTTTGGCGGTGAGTAAGTCGTCGGCGAGTTCAAGAAGCTCAACCCGAAC
GTCCCAACCGACCCGAGGGAGCAGCTCCGTGGCGCCATCCTCGCGGTGTT
CCGGTCCTGGAACAACGACCGCGCCATCCGCTACCGGTCGTACAACGGCA
TCCCTGATGCGTGGGGCACCGCCGTATCGGTGCAGGCCATGGCCTACGGC
AACATGAACGACGGCTGCGGTACGGGGGTGGCTTTCTCGAGGAACCCTTC
CACTGGAGAGAACAAGTTCTTTGGCGGTGAGTAA