mRNA_H-canaliculatus_F_contig1227.1946.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: A0A6H5JB40_9PHAE (Ion_trans domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JB40_9PHAE)

HSP 1 Score: 525 bits (1352), Expect = 1.560e-151
Identity = 483/1630 (29.63%), Postives = 723/1630 (44.36%), Query Frame = 2

Query:    2 EELIEEALQDKDLGDRRNILLYAASMGKDVSFTSAVRAIRERLGRKALVKQLRESDVHGAPLLFQAAASNSRPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVDHLTRDANDGTSWLI-----AKSADDHHGQQGVERICGTAVHVGSKGMNVLHFACRWGCPCIVDKVISEAKKQG---TIFVHEILCTADAVGRTPLMQALRQDHDENCGVRVPKVDVVLKTLTDVANHTDS----------------DTLAHFTQPVTSEHGSTALMHAAYGGPKQLRIVCSKITSLAKSCKIWDKASNTDRNDRGELRLDFALGLEDVVEEEKLRR----------------------YGFLLKEAARGGHIRALETVVHAIKTGVL------RPNGREYGIHDEEGSKRLKSAITARKMN---LLAEAISSRYRSVEMVARIVDLIETHIPQPQEAWNALRGPERDGSNLVFAASMSESLVEQANSADIFRLVYRSLEKAAKGAF--PESYALHLRQLFIPNTPADERPVSSHDSV------AKTPLAAAMLSRDKAVFQEVYDTFHGLSEDTDTQSWTRQQVVQQIPTQVNPEREDMTLWGAESA-------------RAIMNISPTMLLTVIKA-----VKDATPAADEMARRQENDWTTQRLQR-LVQAAAAVGAFDLFRVS-----PFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVGKAFGAHEAALFKLRTNPDMQD-KYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHTIRDY-------------SEQHEGDAR-----HELTSADLLMRFLQGWGEGRNDISSD----GKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPT-HR----------------------VLRSNQDQDSFVPGSTQTGRISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSALHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG-CAL---PLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVG-VVWHVVRGMCSRFVPGPHAVSGDLPAWNSSSSNSSFTQQSRTVAGAVSETYLWDTAIGAVHRGLFAAIVGSLALAASVILWMVSIPWIAWQI----LRKLHPKDDS---------RSSWHTGYARPLVAIILKGTGWALACTVATVLSMTCALGCVALWLCGLWGIGQWWRYAISSDGKGKRPLQRDWAFAEDLVSKRNGDDFDDGWR-----------------QFRVDVLVKKTTGLDVE 4387
            E L+ +AL  +D  D +N++ YAA  G+   F   V+ +R++LG   LVKQLR  D++G PLLF A +S S                                                  VD   R      +W +        A    G  G   I  T   V   G N+LH A   GC  ++ +++   +       + +H+     D  G TPL   LR  + E      P+V   ++   +     +                   L    +   +    T ++HAA GG   L+++ +KI       K W    N   N    +     + + + +E E                          +G LL  AA+GGH+  LE VVHAIKTG        R   + + ++  E  +R+  A+ A   +   L  +A+ S     E V  +   +    P+  E W  +RG + D  N +  AS S    E+    ++   VY  L++AAK  F  PE    +L++LF+  +    +P  S  S       + TPLA A LS +  +FQ++Y+ +  L++    Q W+R+++++ +P       +D  + G   A             R + N++  +   +++A     VK A     EM+       T + L R  V  AA  GAFD  R       P + + IP LL    D EE V+  VM A+  + NP VM A  S+++ +AE + PMHR  LR LQ  VD L  ++L++LPHT+RG+G           +L   P +Q  + + LA   G++V +W+LEP   L R +  G R  G                   D++DPL RALDRGS AL FV+ PL+LDY+  KFS ++P W S +     ++  FYT+T  D               E ++G A      +     D L+RFLQGW     D+S +    G++ +      +PH T+LP LQF+LAG+IG P  FY VPA+R+    L Y+ ML LF A V+++ +  +P  E  FY+F+AG LWRE LEF D +P  HR                            D   +  G     R+++ + RYLF D WN+LD  T+  V  AF+ R+  +        PG                F+ Q  LA SA  LF+R+L LSQID TLGPM QIIWRM+S  ++F   + V+MA FA+ F+ +F  C   S L E + + G A++T+FKA LG F F+ F ++ G C     P  A   G  LLV YL ++++++LNLLIAVLSTAH EV+AN EKEFHLAR +LI QS   V   R PPP NL+   +G + D V  + W  VRG                       ++    +Q +     ++ TY W T  G + R LFA+ +G +A+  + +LW++S+PW+AW+I    L K+    D          R     G  R  + + L       A   A  L   C  G   LWL G+  + +W  + +S +G+      +       +  +  G ++   W+                  F V  L++ TTGLD+E
Sbjct:   39 ESLMHQALLARDPEDGQNLMAYAARWGRKRWFLYLVQHVRDKLGVGCLVKQLRAWDINGTPLLFLAISSRSSENCFKTVYDELVTALGKGGLVEQIAASDDLGRNIVMYAARGNS------VDVFKR------AWKMYHYHQRTFASPVEGWGGAPSI--TLPKVDYTGRNLLHHAAEAGCLDVIHEILLMVRNLSDSTNVTMHK----PDKNGLTPLHHLLRAKYGE------PEVPDDIEDPAEFPQKFEKLWFYGSKGSFMARRKVMPLPRDGRAAVAVSAITDVIHAARGGLPSLQLILAKICLT----KCWQGKPN---NTVVTIEEALCVAVHETMEGEDEXXXXXXXXXXXXXXXXXXELDAWGWGMLLTAAAKGGHVDVLEHVVHAIKTGSFVEGLSDRNADQRHTVYTNEELQRVDRAVEAIVSSGSYLFGQAVLSG--KAEAVLWVYQFLAQRYPK--ELWKNVRGAQ-DKINALTCASSSSMKCER-QGVEVLVAVYSCLQEAAKYEFGTPEDVTKNLKELFLSRS-LPMKPTGSKSSARTTVESSSTPLAGAALSGNWVLFQKIYEMYERLTD----QRWSREELLRHLPI----ASQDKIMLGQCQAMHECDPHVHTSFGRGVGNVTAVIWRDLVEAYQRAEVKCAVIELSEMSDIPSWRDTFKSLSRKAVGLAAERGAFDDLRELVKAGLPLHDDLIPTLLTSAED-EEEVMDIVMYAVGNASNPLVMGAGVSKNLRRAEVDCPMHRAKLRCLQQIVDHLMNQLLDKLPHTVRGMGMTLLRPMVPHHRL---PGLQQHRLDTLANLAGYMVVEWILEPRH-LNRQATVGKRYNGP------------------DYMDPLQRALDRGSKALFFVDSPLVLDYIYVKFSCTLPNWASSSSIPKTINAGFYTYTAFDEYEFWEVLSVRPGPPEANQGGALQPQPWNSKKWDDRLLRFLQGWNP--TDMSPEEQPRGRSTRRSDRNKLPHTTVLPMLQFSLAGLIGKPAVFYNVPAVRFALEFLHYLVMLGLFCACVKLQDSYRIPPAEVAFYVFMAGTLWREILEFCDGVPARHRRXXXXXXXXXXXXXXXXTAAQWASSKRSDTVDYEQGRGLK-RVASAITRYLFYDTWNLLDTLTLSTVFLAFIFRLLGIREFGGPADPG-------------DYFFVAQFFLAASAPLLFARLLRLSQIDSTLGPMIQIIWRMLSQTLRFGVFIVVVMAGFALAFNAVFGSCDAGSDLDESYGSFGTAFLTVFKAPLGEFKFEDFDDVGGQCPYNPSPGRASDAGTFLLVAYLVVLAVVMLNLLIAVLSTAHGEVYANGEKEFHLARTRLILQSARAVAHRRIPPPFNLVQLVVGFLLDGVSELAWWAVRG-----------------------ADRCGLRQHKESFAPITYTYGWHTFDGMLQRLLFASTMGLVAVTLNALLWVLSLPWVAWRIVCWVLYKVVETGDPDEVETFLSVRLRDKKGKFRAFLWVFLGIIACVFALGFAVALCGVCVAGSFVLWLRGVCNVFEWAHWRLSEEGRFVSGETKKGTCPTAMPGQERGSNWRHAWKYVAKARNFRIRHEHDSEHFHVAPLLQATTGLDME 1560          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D8LH99_ECTSI (Ankyrin Repeat Transient Receptor Potential Channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LH99_ECTSI)

HSP 1 Score: 477 bits (1228), Expect = 7.250e-135
Identity = 496/1688 (29.38%), Postives = 708/1688 (41.94%), Query Frame = 2

Query:    2 EELIEEALQDKDLGDRRNILLYAASMGKDVSFTSAVRAIRERLGRKALVKQLRESDVHGAPLLFQAAASNSRPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVDHLTRDANDG------------TSWLIAK--SADDHHGQQGVER-ICGTAVHVGSKGMNVLHFACRWGCPCIVDKVISEAKKQGTIFVHEILCTADAVGRTPLMQALRQDH---DENCGVRVP--------KVDVVLKTLTDVANHTDSDTLAHFTQPV--------------TSEHGSTALMHAAYGGPKQLRIVCSKITSLAKSCKIWDKASNTDRNDRGELRLDFALG-LEDVVEEEKLRRYGFLLKEAARGGHIRALETVVHAIKTG------------------VLRPNGREY-----GIHDEEGSKRLKSAITARKMNLLAEAI-SSRYRSVEMVARIVDLIETHI-PQPQEAWNALRGPERDGSNLVFAASMSESLVEQANSADIFRLVYRSLEKAAKGAFPESYALHLRQLFIPNTPADERPVSSHDSVAKTPLAAAMLSRDKAVFQEVYDTFHGLSEDTDTQSWTRQQVVQQIPTQVNPEREDMTLWGAESA---------RAIMNISPTMLLTVIKAVKDATPAADEMARR------QENDWTTQR------LQRLVQAAAAVGAF----DLFRVS-PFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVG-KAFGAHEAALFKLRTNPDMQDKYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGAL----DFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHTIRDYSEQHEGDARHELTSADLLMRFLQGW-----------------GEGRN--------------------------DISSDGKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPTHRVLRSNQDQDSFVPGSTQTG--------------------RISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSA------------LHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG---CALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHV----VRGMCSRFVPGPHAVSGDLPAWNSSSSNSSFTQQSRTVAGAVSETYLWDTAIGAVHRGLFAAIVGSLALAASVILWMVSIPWIAWQILRKLHP-----------------------KDDS-------------RSSWHTGYARPLVAIILKGTGWALACTVATVLSMTCALGCVALWLCGLWGIGQWWRYAI---SSDGKGKRPLQRD---WAFAEDLVSKRN-GDDFDDGWRQFRVDVLVKKTTGLDVEGLWQ 4399
            E +++EAL  K   D +N+L+YAA+ G +  F   +  IR +LG  ALV +LRE D+ G PLLF AA+SN +                                                 V +  R+  D             T  L  +  + D      G+ R + G A  +G    N LH A   G   ++ +V+          V+E + TAD +GRTP+M  LR D     E  G  +         K   + + L D     D+     + +P                     T LMHAA GG   L +  +K+   +++          D      L ++  +G  ++     K   +  LL  AA+ G I  L  V+ AIK G                  V RP G  +     G   +E  +    AI  R  ++ + AI S R  ++E+V    DL+      +  + W+ L G     S+L  AAS S  +    +   +F  V+  L++       +   L  RQL    +P    P      V  +PL  A  S +  +F++VYDT+    E+     W RQQV+ QIP+     R  + L               R   +I   M   ++KA + A   + +   R      + N   + R       +R V+ AA  G F    DL +   P +   IP LL+ IGD+E+++I  ++  +  + NPF M A  SR +  +E + PMH++GLRRLQ+ +D   +++L++LP T+RG+G +  G H+   F    +   Q K++RL    GFI  QW LEP  +L           GD   ++ Y           D+IDPL RALDRGS  L    DF    L     SE  S  V P T+      + +             Q EG       S   L+R LQGW                  +G+                           +  +     +    + +PH T+LPGLQF+LAGI G P  FY+VP IR+ + + SY+ ML LF  SV ++   ++P  E  FY+F AGLLWRE LEFRD +P  R  +  + Q S   GST+TG                    R+ +   RY+F D WN LD STI  +  AF+ R+  L                D+   L H     Q   A SA  LFSR+L LSQID TLGPMTQ+IWRMMSH ++FSA +A++M SFA+ FH +F  C E S             L + F T GD+++T+F   LG  DF++F+       C LP  A   GI LLV+Y+  M+++LLNLLIAVLST H +V+ANAEKEFH ARA+LI QS   V R R PPP+NLI    G++ D    VW V     RG     +  P                              S T+ W T  G + R  FA   G  A+  S ILW+VS+PW+AW + R +H                        +DD              +   H GY R  ++ I       +A   A+ L +      + LW  G+              + D     P Q D   W  A +L S RN G   + G   F +  L+K  TGLD++ L Q
Sbjct:   31 ESILDEALLSKCPEDGQNLLMYAAAHGNEEWFLHLIDHIRSQLGIPALVTELREIDIDGCPLLFHAASSNCKYSCFRTAKYIILSVLGKGELVQQFELVDGLGRGILMHAARSNHVDNFKEVFNRCREVADRMRPHDEPTVGGQTESLDPQLPATDTASADIGLLREVLGKADRIGK---NCLHHAAEAGSYAVLHEVVKLCCS-----VYEEMNTADIMGRTPIMYVLRNDSCRGQEQQGSTLALKEESLRKKFHELYEALQDATCPQDTPVKTGWMEPSRVPPPSLAVPKGTRVDTRAVTELMHAARGGLVSLELALNKLPLASRNIAEGGFTVALDNA----LAVNMTIGNTQEQSHRTKAWGWALLLAAAAKLGDIDVLYHVLLAIKKGKFTLVKHGENDHDFRLAKVERPAGGGWVELPRGDRVQEAVR----AINTRGKSVFSYAILSGRTDAMELV---YDLVRGLFGDKTSQTWDTLSGKTNKSSSLTCAASAS--MEADNHGITMFDKVFSLLQQQT-----DDRLLVQRQL----SPRFAMPHRGKGKVKISPLVGAAFSSNWILFRKVYDTY----EEWTGHRWWRQQVLAQIPSGPPRRRSVLELQEMHDCEDDIPALIIRGAWSIPAVMWRDIVKAYQRAQLCSPDATSRGVPQPLRINPLPSWRDEFKGYSRRAVKEAAKRGTFRSLRDLVQEGFPLHEHHIPALLENIGDHEKDIIETILATVTNASNPFAMGAGLSRTLRTSEVDHPMHQLGLRRLQSIIDDFTMELLDKLPRTVRGMGMELIGGHQPITFGEGFH---QGKHHRLGNLAGFIAVQWFLEPRVLL-----------GDVAVENRYKE--------PDYIDPLRRALDRGSKGLYKYDDFETYKL-----SEILSTPVKPPTANQEGEQSPA-------------QKEG-----FISMTFLLRLLQGWDHRDPKRRCNFPSRVFRAKGQEASGAQAXXXXXXXXXXXXXXPVSAAANAQAPASRPEPPNQWRIPHSTMLPGLQFSLAGIQGKPETFYKVPVIRFAFEIFSYLVMLVLFCFSVLLKEHDSIPWGEVTFYVFAAGLLWREILEFRDGMPARRH-KPPKRQVSKNDGSTRTGSEQPLSFPRISRLGTGNRFNRMVSAFTRYVFYDTWNFLDTSTIGCILVAFIFRMIAL----------------DDKFFLFHA----QFFYALSAPLLFSRLLVLSQIDATLGPMTQVIWRMMSHTLRFSAFIAMVMLSFALAFHAVFHTCGEYSEPQCTLDDDDAFPLRDAFGTFGDSFVTVFTYALGGPDFEAFQLAGSDCRCDLPEGARNAGIFLLVVYMITMTVVLLNLLIAVLSTTHGKVYANAEKEFHHARARLIYQSARVVSRSRPPPPLNLIKLVCGILLDAGTEVWRVGVWVTRGRSQARMLKPF-----------------------------STTHQWKTLEGGLQRLEFAFTFGVAAVGLSTILWIVSVPWVAWCLHRLVHKAEEETXXXXXXXXXXXXXXXXXERDDGXXXXXXXXXXXXXKEKPHLGYFRRFLSGIKSMCSLLVAMVAASFLCLLYIFATMILWAVGIXXXXXXXXXXXDKKNGDMPEPMPGQEDGHHWKRAWELASLRNQGARANRGQAHFHIAPLLKSKTGLDMQHLAQ 1589          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D8LHA0_ECTSI (Transient Receptor Potential Channel. Partial sequence n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHA0_ECTSI)

HSP 1 Score: 427 bits (1097), Expect = 1.980e-117
Identity = 493/1820 (27.09%), Postives = 703/1820 (38.63%), Query Frame = 2

Query:    2 EELIEEALQDKDLGDRRNILLYAASMGKDVSFTSAVRAIRERLGRKALVKQLRESDVHGAPLLFQAAASNSRPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVV--------------DHLTRDANDGTSWLIAKSADDHHGQQG----------------VERICGTAVHVGSKGMNVLHFACRWGCPCIVDKVISEAKKQGTIFVHEILCTADAVGRTPLMQALRQ--------------DHDENCGVRVPKVDVVLKTLTDVANHT----------DSDTLAHFTQPVTSEHGSTALMHAAYGGPKQLRIVCSKITSLAKSCKIWDKASNTDRNDRGELRLDFALGLEDVVE---------EEKLRRYG--FLLKEAARGGHIRALETVVHAIK----------TGVLRPNGREYGIHDEEG----SKRLKSA---ITARKMNLLAEAI-SSRYRSVEMVARIV-----------------------DLIETHIPQPQE----------AWNALRGPERDGSNLVFAASMSESLVEQANSADIFRLVYRSLEKAAKGAFPESYALHLRQLFIPNTPADERPVSSHDSVAKTPLAAAMLSRDKAVFQEVYDTFHGLSEDTDTQSWTRQQVVQQIPTQVNPEREDMTLWGAESARAIMNISP-----------TMLLTVIKAVKDATPAADE---MARRQENDWTTQR------LQRLVQAAAAVGAFDLFRVS-----PFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVGKAF-GAHEAALFKLRTNPDMQDKYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTH-----------------TIRDYSEQHEG---DARHELTSADLLMRFLQGWGE-------------------------------------------------------------------------------------GRNDISSDGK---------------------NDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPTHRVLRSNQDQDSFVPGSTQTGRISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETS------------ALHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG---CALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVV-----WHVVRGMCSRFVPGPHAVSGDLPAWNSSSSNSSFTQQSR--------------TVAG-------AVSETYLWDTAIGAVHRGLFAAIVGSLALAASVILWMVSIPWIAWQILRKLHP-------------------------KDDSRSSWHTGYARPLVAIILKGTGWALACTVATVLSMTCALGCVALWLCGLWGIGQWWRY---AISSDGKGKRPLQRD---WAFAEDLVSKRNG---DDFDDGWRQFRVDVLVKKTTGLDVEGL-WQRMKRKEKGLS 4429
            E L++EALQ K   D +N+LLYAA+ G        V  IR R+G  ALVK+LRE+D+ G PLLF AA+S  +                                                 V              D     ANDG   +         G++                 +  + GT   V  KGM  LH A   G   ++ +V+ E    G+ +    L  +D  GRTP+M  LR               D ++  G+   ++   L+ +     H               L    Q        T L+HAA GG   L +  +K    +K          TD  D  ++ LD AL +E  ++           +   +G   LL  AAR G +  L  V+ AI+           G  R   R   + +       S +L+     I +R ++L + AI S R+ +VE++  +V                       D  + H  Q Q            W+ L G     S+L  AAS+S  +    N   +F LV+  LEK  +    E      R+L  P +    +  S+    + +PLA A+ S +  +F+EVYD +  L+       W R+ V++QIP    P      L   E      NI P            M   ++KA K   P   E      RQ     + R       +R  + AA  G F   R       P + + +P LL+ IGD+E +VI  ++ A+  + NPF M A  SR +  ++ + PMH  GL RLQ+ +D    K+LE+LP T+RG+G A  G H+  +F        Q K +RL    GFIV QW+LEP+ ++ +++ +             YA          D++DPL RALDRGS ALDF+N PL+ DY+  KF+ ++P WTS  PF   V+E F+ +                  +    E+  G     + +  +   L+RFLQGW                                                                                        R D                           ND+  RW  +PH T+LPGLQF+LAG++G P  FY+VPAIR+ + + +Y+                                                                           Y+F D WN LD STI  +   FV R+  L                D+   L H     Q   A SA  LFSR+L LSQID TLGPMTQ+IWRM+S  ++FSA ++V+M SFA+ FH +F  C E               L + F T G +++T+F A LG  DF+ +        C LP  A+  GI L+V+Y+ +M+++LLNLLIAVLSTAH++V+ N +KEFHLARAKLI QS   V R R PPP+NL+   LG + D          W V           P   +           N +   ++                +AG       A ++T+ W T  G V R LFA   G+ A+  S ILW +S+PW+AW +LR  HP                         +++ +   H GY R +    L      +A  VA +L     L  V LW  GL  I +W R      S+D     P Q +   W  A DLVS RN    +DFD     F V   +K  TGLD + L W    R +K  S
Sbjct:   38 ESLLDEALQSKWPEDGQNVLLYAAAQGNQTWLLGLVDLIRSRVGIHALVKELRETDIDGVPLLFHAASSRRKHCCFRIAYDLVFTVLGKGGVIEQFEALDGLGRGILMHAARSNHVDTFKDVFDKCRKAAESVRLHDQSNAVANDGRIEIPEVPLYGFRGERPSDSTTQTPEKSSNSLLLREVLGT---VDRKGMTCLHHAAEAGSSEVLCEVVKEC---GSHWAD--LGASDRSGRTPIMLVLRNAFCCEGEEHTCRQTDLEDKFGILFDEIP--LEPIRATGGHRYIGWMQPSRIQPQRLPAPKQTPVETRAVTELIHAARGGVVSLELALNKALPNSK----------TDSEDGFKVHLDSALAVEVCIDGSWRQSQDDHHRTVIWGRALLLAAAARLGDVDVLYHVLVAIEDDEFFLEADDAGKFRLPERPIPVDETPTAIPVSNKLREVLGMINSRNVSLFSLAILSRRHEAVELIFDLVVRLFGEDQSSRNDQRAVRFRDGDDGDQGHQGQGQRDRGNQPHHDTIWSILTGHSHTTSSLTCAASVS--MEADGNGRTMFCLVFEMLEKQFRRGVGEK---KFRELLSPKS-MTLKSSSTDQEHSISPLAGAVFSSNWVLFREVYDRYEKLA----GCCWQRETVLKQIPQ--GPPHGRSVLKDEELHEYHDNIPPLITRGAWTIPAVMWPDIVKAYKRGEPDDREGYCCCGRQHQRLLSWRDDFKHYSRRGAKVAAKRGDFTALRELVNEGFPLHDDHMPFLLENIGDHEHDVIDTILAAVASTSNPFAMAAGVSRTLRMSKVDFPMHSEGLGRLQSIMDDFTSKVLEQLPQTVRGMGVALLGGHQPIVFGEGLE---QGKRHRLTNLAGFIVVQWILEPNLLVGKINTQKK-----------YA--------GPDYLDPLRRALDRGSEALDFLNTPLVKDYVHIKFACTLPSWTSRNPFQPTVNEGFFKYDDFDTYPLSEVFSAPDENLGSKDEESRGLKAALKEQFLTKSFLLRFLQGWDHRDSLLKWNIPIGDIRFASKKFKSDTRASNNXXXXXXXXXXXXXXXXXXXXXPGCCGGRFKSIIPSWLLCCNGNVSGNAXXXXXXNARGDAHDSSAHXXXXXXXXXXXSSCRQPLWFNDKPKRWT-IPHSTILPGLQFSLAGVLGKPETFYQVPAIRFVFEIFTYL---------------------------------------------------------------------------YVFYDTWNFLDTSTIGCLLVTFVFRMIAL----------------DDDFFLFHA----QFFYAASAPLLFSRLLVLSQIDATLGPMTQVIWRMISDTLRFSAFISVVMLSFALAFHAVFHTCGEFEDPTCNTNDDPDFPLRDAFGTFGRSFVTVFSAALGGPDFELYEAAGSECRCKLPDGAQEAGIFLMVVYMVIMAVVLLNLLIAVLSTAHSKVYDNGDKEFHLARAKLIYQSARVVTRRRPPPPLNLVKVVLGTLIDATTEPVRFRRWMVRNPPEDENEANPADGAXXXXXXXXGHENDAVEGEAEGDQGNIGGGSPGDCPIAGTPGDEFIAWTQTHEWRTFEGGVQRVLFAFTFGAAAIVMSAILWTLSVPWVAWCLLRLAHPVVEKRAENLEDEEDDTNQGLGDIEEEEEMKEKVHPGYFRRIFITFLWSAVLVVATAVAAILCALYILFSVVLWFVGLHMIYKWVRTNWTPESNDRPRAMPGQEEGDHWQRAWDLVSLRNHGVREDFDQA--HFNVLPFLKSKTGLDRQHLAWLTKARDDKAKS 1705          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D7FX79_ECTSI (Ankyrin Repeat Transient Receptor Potential Channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX79_ECTSI)

HSP 1 Score: 416 bits (1070), Expect = 3.980e-117
Identity = 394/1357 (29.03%), Postives = 600/1357 (44.22%), Query Frame = 2

Query:    2 EELIEEALQDKDLGDRRNILLYAASMGKDVSFTSAVRAIRERLGRKALVKQLRESDVHGAPLLFQAAASNSRPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVDHLTRDANDGTSWLI-----AKSADDHHGQQGVERICGTAVHVGSKGMNVLHFACRWGCPCIVDKVISEAKKQGTIFVHEILCTADAVGRTPLMQALRQDHDENCGVRVPKVDVVLKTLTDVANHTDS----------------DTLAHFTQPVTSEHGSTALMHAAYGGPKQLRIVCSKITSLAKSCKIWDKASNTDRNDRGELRLDFALGLEDVVEEEKLRR----------------------YGFLLKEAARGGHIRALETVVHAIKTGVL------RPNGREYGIHDEEGSKRLKSAITARKMN---LLAEAISSRYRSVEMVARIVDLIETHIPQPQEAWNALRGPERDGSNLVFAASMSESLVEQANSADIFRLVYRSLEK--------------------------AAKGAFP--ESYALHLRQLFIPNTPADERPVSSHDSV------AKTPLAAAMLSRDKAVFQEVYDTFHGLSEDTDTQSWTRQQVVQQIPTQVNPEREDMTLWGAESA-------------RAIMNISPTMLLTVIKAVKDATPAADEMARRQE--------NDWTTQRLQRLVQAAAAVGAFDLFRVSPFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVGKAFGAHEAALFKLRTNPDMQD-KYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHTIRD-YSEQHEGDARHELTSA---------------DLLMRFLQGWGEGRNDISSD----GKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPT-HRVLRSNQ--------DQDSFVPGSTQTG-------------RISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSALHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG-CAL---PLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEV 3610
            E L+ +AL  +D  D +N++ YAA  G++  F   V+ +R++LG   LVKQLR  D++G PLLF A +S S                                                  VD   R      +W +        +    G  G   I  T   V   G N+LH A   GC  ++++++   +K      +  +   D  G TPL   LR  + E      P+V   ++   +     +                   L    +   +    T ++HAA GG   L+++ +KI       K W    N   N    +     + + + +E E                          +G LL  AA+GGH+  LE VVHAIKTG        R   + + ++  +  +R+  A+ A   +   L  +A+ S    VE+V  +   +    PQ  E W  +RG +   + L  A+S S     Q    ++   VY  L++                          AAK  F   E    +L+ LF+  +   + P  S  S       + TPLA A LS +  +FQ +Y+ +  L++    Q W+R+++++ +P       +D  + G   A             R + N++  +   +++A + A   + E+   +E         D      ++ V+ AA  GAFD  R      E +   L L  D    ++++         NP VM A  S+++ +AE + PMHR  LR LQ  VD L  ++L++LPHT+RG+G           +L   P +Q  + + LA   G++V +W+LEP  +L R +  G R  G                   D++DPL RALDRGS ALDFV+ PL+LDY+  KFS ++P W S +     ++  FYT+   D Y+       R E   A               D L+RFLQGW     D++ +    GK+ +      +PH T+LP LQF+LAG+IG P  FY VPA+R+    L Y+ ML LF + V+++ +  +P  E  FY+F+AG LWRE LEF D +P  HR  R N+        +  + V G++                R+++ + RYLF D WN+LD  T+  V  AFV R+  +      T PG                F+ Q  LA SA  LF+R+L LSQID TLGPM QIIWRM+S  ++F   + V+MA FA+ F+ +F  C   S L E + + G A +T+FKA LG F F+ F ++ G C     P  A   G  LLV YL ++++++LNLLIAVLSTAH E+
Sbjct:   30 ESLMHQALLARDPEDGQNLMAYAARWGREEWFLYLVQHVRDKLGVGCLVKQLRAWDINGTPLLFLAISSRSSENCFKAVFDELVTALGKGGLVEQIAASDDLGRNIVMYAARGNS------VDVFKR------AWKMYHYHQRTFSSPVEGWGGAPSI--TLTKVDYTGRNLLHHAAEAGCLDVLNEILLMVRKLSDS-ANGTMHKPDKNGLTPLHHLLRAKYGE------PEVPGDIEDPAEFPQKFEKLWLYGSKGSFMARRKVMALPRDGRADVAVSAITDVIHAARGGLPSLQLILAKICLT----KFWQGKPN---NTVVTIEEALCVAVHETMEGEDEXXXXXXXXXXXXXXXXXXELDAWGWGMLLAAAAKGGHVDVLEHVVHAIKTGSFVEGLSDRNADQLHTVYTNQELQRVDRAVEAIVSSGSYLFGQAVLSG--KVEVVLWVYQFLAQRYPQ--ELWKNVRGAQDKINALTCASSASMKCERQ--GVEVLVAVYSCLQEVAEKYLICVTSGHIKRVHQVRDSRREAAKCEFKTHEDITRNLKDLFVSRSHTMQ-PAGSKSSARTTVKSSSTPLAGAALSGNWVLFQRIYEMYERLTD----QRWSREELLRHLPI----ASQDKIMLGQCQAMHECGRHVHTSFGRGVGNVTAVIWRDLVEAYQRAEVTSAEIEGSEEVISDIPSWRDTFKSLSRKAVRLAAERGAFDDLR------ELVKAGLPLHDDLIPTLLTS---------NPLVMGAGVSKNLRRAEVDCPMHRAKLRCLQRIVDHLINQLLDKLPHTVRGMGMTLLRPMVPHHRL---PGLQQHRLDTLANLAGYMVVEWILEPR-LLNRQATVGKRYNGP------------------DYMDPLQRALDRGSKALDFVDSPLVLDYIHVKFSCTLPNWASSSSIPKTINAGFYTYNAFDEYNFSEVLSVRPEPPEANPGGGSQPCNSKKWDDRLLRFLQGWNP--TDMNPEEQPRGKSTRRSDRNKLPHTTILPMLQFSLAGLIGKPAVFYNVPAVRFALEFLHYLVMLGLFCSCVKLQDSYRIPPAEVAFYVFMAGTLWREILEFCDGVPARHRRHRKNKHIKEGNQANMATAVQGASSKRSDMVDYGQGRGLKRVASAITRYLFYDTWNLLDTLTLSTVFLAFVFRLLGIREFGGPTDPG-------------DYFFVAQFFLAVSAPLLFARLLRLSQIDNTLGPMIQIIWRMLSQTLRFGVFIVVVMAGFALAFNAVFGSCDAGSGLDESYGSFGTALLTVFKAPLGEFKFEDFDDVGGQCPYNPSPGRASDAGTFLLVAYLIVLAVVMLNLLIAVLSTAHGEL 1291          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D8LH97_ECTSI (Ankyrin Repeat Transient Receptor Potential Channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LH97_ECTSI)

HSP 1 Score: 404 bits (1039), Expect = 1.140e-113
Identity = 358/1182 (30.29%), Postives = 516/1182 (43.65%), Query Frame = 2

Query:  488 KGMNVLHFACRWGCPCIVDKVISEAKKQGTIFVHEILCTADAVGRTPLMQALRQDHDENCGVRVPKVDVVLKTLTDVANHTDSDTLAHFTQPVTSEHGSTALMHAAYGGPKQLRIVCSKITSLAKSCKIWDKASNTDRNDRGELRLDFALGL---EDVVEEEKLRRYGFLLKEAA-RGGHIRALETVVHAIKTGVLRPNGREYGIHDEEGSKRLKSAITARKMNLLA-EAISSRYRSVEMVARIVDLIETHIPQPQEAWNALRGPERDGSNLVFAASMSESLVEQANSADIFRLVYRSLEKAAKGAFPESYALHLRQLFIPNTPADERPVSSHDSVAKTPLAAAMLSRDKAVFQEVYDTFHGLSEDTDTQSWTRQQVVQQIP-------TQVNP------EREDMTLWGAESARAIMNISPTMLLTVIKAVKDATPAADEMARRQENDWTTQRLQRLVQAAAAVGAF----DLFRVS-PFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVGKAFGAHEAALFKLRTNPDMQDKYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHT---IRDYSEQHEGDARHELTSA----------------------------DLLMRFLQGWGEGRNDISSDGKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPTHRVLRSN----QDQDSFVPGSTQTG------RISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSA------------LHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG---CALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHVV---RGMCSR 3787
            KGMN LH A   GC  ++ KVI +    G     E     D   RTP+M  L     E       + D  L+    +     S T     + V +  G     HA  G  +   +   +I+   +  K             G + L+ AL        +E+  +   G L  EA               A   G+L     E G  D      L   +TA K N    E  + R+   +  +R+ +L        ++    +R   + G +L   A +S   V         + VYR + K      PE++A+   + F     A+  P++   S +  P         +  F  VY      + D   +   R Q+V Q P       TQ++P          +  W        +   P +    ++         +E+   +E  +      R V  A+  GAF    DL +   P + + IP LL  IGD+E+++I  V+ A+  + NPF M A  SR + +A  + PMH+ GLRRLQ  +D+                                                F + QWMLEPS +++++      N     R   Y              DPL  ALDRGS AL+F+  PL+LDY+  KF+ ++P W+S  PF   ++E  Y +    + D ++   G    + T                                L+RFLQGW   R+       NDQ V  + +PHLT+LPGLQF+LAGI+G P  F++VP IR+ Y + SY+ ML LF +SV ++    +P  E IFYIF AGLLWRE LEF D +PT R   S     ++     PG +  G      R+ +   RY+F D WN +D STI  +  AF+ R+        +                    F  Q   A SA  LFSR+L LSQID TLGPMTQ+IWRMMSH+++FSA +A++M SFA+ FH +F  C E S             L + F T GD+ +T+F + LG  +FD F +      C LP  A + GI+++V+Y   MS++LLNLLIAVLSTAH EV+ NAEKEFHLARA+L+ QS   V   R PPP+NL+  G G++ DV   +W  +   R + SR
Sbjct:  189 KGMNCLHHAAEAGCSEVLGKVIKKYSDAGGSSYAE----RDNNNRTPVMCVLSDASREE------RADDSLRDKFTLLYSNMSTTCGEQDEKVCTHDGG----HAKQGWMEMTDVPTQQISRSEEISKARGVTELMHAARGGLVSLELALNEPLPSSRLEDGSISLDGALNVEACDEPASDEPRWEPQPAWGKGLLLAAAAERGDIDV-----LHVVLTAIKDNKFTKEPNTDRFTLRKQESRLAEL---PYGDGEKVRTVVREMSKGGKSLFSEAILSGQTVA-------VKHVYRLIVKLFGKTHPETWAILTGRDFNSGKVAEASPLACAASASMEPC-----DHGRDTFDLVYSYLQTHAPD---EKKLRIQLVPQPPPLDLTAKTQISPLVGAAFSSNWILFWRVYDTYTTLAKKPWLRTNALRRATSDKSGGEELMSWRE--YLRAYSSRAVHVASERGAFTDLRDLVKEGFPLHDDHIPALLVSIGDHEQDIIETVLFAVANASNPFGMAAGVSRFLRRARVDHPMHQRGLRRLQTRIDR------------------------------------------------FTLEQWMLEPSLLVEKI------NKAKKYRGPVYK-------------DPLQSALDRGSDALEFLFSPLVLDYVHVKFAGTLPHWSSRNPFQPTINEGLYKYDNFEVYDLAKLLRGSKNDQATGRTAPSGASSASTGSTNRGSFLWRSPGSIMSFLLRFLQGWDHPRS-------NDQSV--WSIPHLTILPGLQFSLAGILGKPETFFKVPVIRFVYEIFSYLVMLVLFCSSVLLKEPDFIPRDEVIFYIFAAGLLWREVLEFLDGVPTRRHRTSGGPRTEEGGRVEPGISHLGAGSNFNRMVSAFTRYVFYDTWNFMDTSTICCIFVAFIFRMIARFDSEFDL-------------------FYAQFFYALSAPLLFSRLLVLSQIDATLGPMTQVIWRMMSHMLRFSAFIAMVMLSFALAFHAVFHTCGEHSEPQCTLDDDDAFPLRDAFGTFGDSLLTVFSSALGGPEFDLFDDAGSDCRCNLPRGARSAGISMMVVYTITMSVVLLNLLIAVLSTAHDEVYVNAEKEFHLARARLVVQSARSVAHRRPPPPLNLVKLGFGVLIDVFTEIWRFILWARSLRSR 1236          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: A0A6H5JTT3_9PHAE (Ion_trans domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTT3_9PHAE)

HSP 1 Score: 350 bits (898), Expect = 1.390e-97
Identity = 227/586 (38.74%), Postives = 312/586 (53.24%), Query Frame = 2

Query: 2216 QWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHT---IRDYSEQHEGDARHELTSAD-------------------------------LLMRFLQGWGEGRNDISSDGKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPT--HRVLRSNQDQ--DSFVPGSTQTG------RISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSA------------LHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEG---CALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHVV---RGMCSR 3787
            QWMLEPS +++++      N     R   Y              DPL  ALDRGS AL+F+  PL+LDY+  KF+ ++PPW S  PF   ++E F+ +    + D ++   G    +    +                                L+R LQGW   R+       N+ +  W+ VPHLT+LPGLQF+LAGI+G P  F++VP IR+ Y   SY+ ML LF +SV ++    +P  E IFYIF AGLLWRE LEF D +PT  HR+ R    +      PG +  G      R+ +   RY+F D WN +D STI  +  AF+ R+        +                    F  Q   A SA  LFSR+L LSQID TLGPMTQ+IWRMMSH+++FSA +A++M SFA+ FH +F  C E S             L + F T GD+++T+F + LG  + D F ++     C LP  A + GI+++V+Y   MS++LLNLLIAVLSTAH EV+ NAEKEFHLARA+L+ QS   V   R PPP+NLI  G G++ D+   +W  +   R + SR
Sbjct:   66 QWMLEPSLLVEKI------NKAKKYRGPVYK-------------DPLQSALDRGSDALEFLFSPLVLDYVHVKFAGTLPPWWSRNPFQPTINEGFFEYDDFEVYDLAKLLRGSGNDQAKGGNEPSGTPTASTGSTAGESLGSFLWQLPGSFMSFLLRVLQGWDHPRS-------NESV--WYYVPHLTILPGLQFSLAGILGKPETFFKVPVIRFVYESFSYLVMLVLFCSSVLLKEPDFIPRDEVIFYIFAAGLLWREVLEFLDGVPTRRHRISRGLGPEVGGRVKPGISHLGAGSNFNRMVSAFTRYVFYDTWNFMDMSTICCILVAFIFRMIARFDSEFDL-------------------FYAQFFYALSAPLLFSRLLVLSQIDATLGPMTQVIWRMMSHMLRFSAFIAMVMLSFALAFHAVFHTCGEYSEPQCTLDDDDPFPLRDAFGTFGDSFVTVFSSALGGPEIDLFDDVGSDCRCNLPRGARSAGISMMVVYTITMSVVLLNLLIAVLSTAHDEVYVNAEKEFHLARARLVVQSAQSVAHLRPPPPLNLIKLGFGVLIDLFTEIWRFILWARSLRSR 604          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D7FMR2_ECTSI (Ion_trans domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMR2_ECTSI)

HSP 1 Score: 357 bits (916), Expect = 8.190e-96
Identity = 271/780 (34.74%), Postives = 396/780 (50.77%), Query Frame = 2

Query: 1814 AAAVGAFD----LFRVSPFYHEAIPCLLDLIGDYEENVISAVMLAMKCSRNPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVDKLGLKILEELPHTIRGVG-KAFGAHEAALFKLRTNPDMQD----------KYNRLATKMGFI-------VTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFA----------WLPSW----NMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHTIRD--YSEQHEGD-------ARHELTSADLLMRFLQGWGEGRNDISSDGKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPTHRVLRSN---------------QDQDSFVPGST-------QTG----RISTTLYRYLFEDAWNILDASTILLVASAFVLRVF----DLHRLSIETKPGANDNGNDNGNNLV-HVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFC--KCQETSALHEQFNTVGDAYITMFKAMLGTFDFDSF---RNIEGCALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHVVRGMCSRFVPG-----PHAVSGDLPAWNSSSSNSSFTQQSRTVAGAVS 3895
            AAA G FD    L       H  +  L+  IGD EE VI+ VM A++ S NPFV  A+AS  + K   +    R+GL RLQ E+D+   ++L +LP T++GVG +   + E         PD ++          + N +A  +G+I       +  W       +K+++          ++  D   FA          WL S      +  +DPL +AL +GS A DF+N PL+LDY+  K+  + P      P  + ++E F T+   +  Y+   +G         R   T+ + L+R LQGW  G   +    K   ++   G PH T+LPG QF LAG++G PT F+ VP +R+ +   SY+ ML L+ +SV I+ +  +P  E +FY+F AGLL+RE LEFRD LP      S+               +D  S  P S        ++G    R+++ L RY  +D WNILD ++I  V  AF+ R+     D  + +              GN L+    F+ +  LA  A  LF+RVLSL+Q+D T GPMTQIIW M+ HL +FS  + V+M SFA+ FH ++    C E   L E + T+ ++ + MF++MLG  +FD F   RN+  C  P      GITLL +Y+ + +IL+LNLLIAVLST H  V  NA  EFHLAR++ IQ S   V+ GR PPP+NL+M  + ++ DV G + H V G   RF+ G     P A S  +PA   + S+     + R   G +S
Sbjct:  943 AAAEGTFDGLRALIEKGWNCHAHLATLIGDIGDAEEQVIATVMCAVENSSNPFVTSAIASVCMAKHGRDCSKDRLGLARLQEEIDQFTTELLGKLPQTVKGVGLELISSFEQPDLGKNDEPDGEEEPATEAELARRKNIMANHVGYIAVVVHCLILDWEGGGIKDIKQIAIAS-------QQALDEKAFALCSVLGACVSWLVSKMISATLQDLDPLQQALTKGSDAHDFINAPLVLDYLELKWKCTTPNLDRRNPLDYTINEGFSTYRSGNGKYTLPEKGVDIGDTFLERGGTTAEESLLRLLQGWDRGPTRVLRF-KEKHLLH--GFPHTTVLPGTQFLLAGLLGKPTDFFLVPFVRFMFETFSYLVMLGLYWSSVAIQYSDIIPADEILFYLFAAGLLFREILEFRDALPPSVSSSSSPTSSPSDATMSSDDGEDHGSSEPDSLAARAREERSGLSQRRVTSGLRRYFSKDNWNILDTASIFCVMLAFIFRIIAYIADFAQAATTIL----------GNTLIGQAFFIARFSLAAIAPLLFARVLSLAQVDNTFGPMTQIIWNMLFHLARFSVFMLVVMVSFALAFHSLYSDPNCSEGDELFESYRTLRESLLDMFRSMLGDANFDRFEADRNLT-CTGPSWEFDAGITLLTIYIVLQAILMLNLLIAVLSTVHDTVSENAITEFHLARSQFIQASAASVEGGRLPPPLNLLMAAILIVVDVFGEICHWV-GKSLRFLSGCGKARPVASSAAVPASGGAGSDPRRRPEERKGPGMIS 1700          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: A0A6H5K4M1_9PHAE (Ion_trans domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K4M1_9PHAE)

HSP 1 Score: 278 bits (711), Expect = 3.690e-74
Identity = 275/916 (30.02%), Postives = 377/916 (41.16%), Query Frame = 2

Query: 1952 NPFVMCAVASRHIEKAESELPMHRIGLRRLQAEVD-------------KLGLKI-----------LEELPHTIRGVG-KAFGAHEAALFKLRTNPDMQDKYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNVSEHFYTHT--------------IRDYSEQHEGDARHELTSADLLMRFLQGWGE----------------GRNDISSDGKNDQIVRWFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFVAGLLWRETLEFRDFLPTHRV-------------LRSNQDQDSFVPGSTQTG------RISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQE------------TSALHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEGCALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHVV----RGMCSRFVPGPHAVSGDLPAWNSSSSNSSFTQQSRTVAGAVSETYLWDTAIGAVHRGLFAAIVGSLALAASVILWMVSIPWIAWQILRKLHPKDDSRSSWHTGYARPLVAIILKGTGWALACTVATVL-SMTCALGCVA---LWLCGLWGIGQWWRYAISSDGKGKRPLQRDWAFAEDLVSKRNGDDFDDGWRQFRVDVLVKKTTGLDVEGLWQRMKRKE 4417
            NPF M A  SR +  +E + PMH++GLRRLQ+ +D             +L L +           L + P  +RG+G +  G H+   F    +   Q K++RL    GFI     L  S ++   + R +      +R   ++R+        D+IDPL R LDRGS  LD VN PL+ DY+  KF+ ++P WTS  PF   V+E  Y +               ++  +   EG+         + M FL                        +  +     +      +PH T+ PGLQF+LAGI G P  FY+                                            GLLWRE LEFR+ +P  R               R+  +Q    P  ++ G      RI +T    +F D W  LD  TI  +  AF+ RV  L                D+   L H  F      A SA  LFSR+L LSQID TLGPMTQ+IWRMMSH ++FSA +A++M SFA+ FH +F  C E               + + F T GD+++T+F                 C LP  A   GI LLV+Y+  M+++LL LLIAVLST H +V+ NAEKEFH ARA  I QS   V R R PPP+NLI    G++ D   ++W       RG     +  P + S                 Q +T+ G +              R  FA   G  A+  S ILW+VS+PW+    L  L  K    +SW       L+  I  G     +  +A V  S  C L   A   LW  G+W I  W   A                 AE                 FRV  L+K  TGLD++ L Q  K ++
Sbjct:   83 NPFAMGAGLSRTLRTSEVDHPMHQLGLRRLQSIIDDFTKEACESIKSPRLVLNVPRGVSVSQRAALGQAPTPVRGMGTELIGGHQPITFGEGFH---QGKHHRLGNLAGFIAVA--LSESRIVATNTGRVAN-----KRVTSHSRYK-----EPDYIDPLRRVLDRGSKGLDMVNSPLVRDYVHVKFTGTLPHWTSRNPFQPIVNEGLYKYDDFETYGLSEILSTPVKPPTANQEGEHSPAQKQGFISMTFLLTLPSQAPTPPMPVPPVQPVSATTNAQAPACRQKPTNQRRIPHSTMPPGLQFSLAGIQGKPHTFYK--------------------------------------------GLLWREILEFRNGVPARRHKPPKRQVPKNEDNTRAGSEQSLSSPRISRPGTRNRFNRIVSTFTWNVFYDTWKFLDTPTIGCILVAFIFRVTTL----------------DDKFFLFHARFF----YALSAPRLFSRLLVLSQIDATLGPMTQVIWRMMSHTLRFSAFIAMVMLSFALAFHAVFHTCGEHIEPQCTFDDDDAFPIRDAFGTFGDSFVTVFTDCR-------------CNLPEGARNAGIFLLVVYMVTMTVVLLKLLIAVLSTTHGKVYDNAEKEFHHARACFIYQSARVVSRSRPPPPLNLIKLVCGILLDAGTMLWRAGAWFRRGRSQAKMLKPFSTS----------------HQRKTLEGGL-------------QRLEFAFTFGVAAVGLSAILWIVSVPWVD-SCLHCLVHKAGEAASW-------LLQTIFSGIRSMFSLLIAMVAASFLCVLYVFATMILWAVGIWMIVTWGLGACQP--------------AESRARANRAQ------AHFRVAPLLKSKTGLDMKHLAQLTKNRD 849          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D7FNX2_ECTSI (Ion_trans domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNX2_ECTSI)

HSP 1 Score: 185 bits (470), Expect = 2.630e-45
Identity = 138/417 (33.09%), Postives = 208/417 (49.88%), Query Frame = 2

Query: 3263 MTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSALHEQFNTVGDAYITMFKAMLGTFDFDSFRNI-EGCA---LPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGLIADVVGVVWHVVR---GMCSRFVPGPHAVSGDLPAWNSSSSNSSFTQQSRTVAGAVSETYLWDTAIGAVHRGLFAAIVGSLALAASVILWMVSIPWIAWQILRKLHPK------DDSRSSWHTGYARPLVAIILKGTGWALACTVATVLSMT----CALGCVALWLCGLWGIGQWWRYAISSDGK---GKRPLQ-----------RDWAFAEDLVSKRNGDDF--DDGWRQFRVDVLVKKTTGLDVEGLWQRMKRK 4414
            M QIIWRMMS  ++F   + V+MA FA+ F  +F  C   S L E F + G A++++F+A LG F F+ F ++   C     P  A   G  LLV YL ++++++LNLL+AVL+TAH EV+AN EKEFHLAR +LI QS   V   R PPP+NL+   +GL+ D VG +   VR   G C     G     G +P                     ++ TY W T  G + R  F   +G +A+A + +LW++S+PW+AW+++  +  +      DD     +    R  +   L   G   AC +A + +      C +G   LW  G+W + +W R+  S +GK   G+   +           ++W  A   V+K        + G   F V  L++ TTGLD++ L    K+K
Sbjct:    1 MIQIIWRMMSQTLRFGVFIVVVMAGFALAFTTVFGSCNADSLLGESFGSFGTAFLSVFEAPLGEFSFEDFHDVGTQCPDNPAPGRASDAGTFLLVAYLVVLAVVMLNLLVAVLTTAHGEVNANGEKEFHLARTRLILQSARAVAHRRIPPPLNLVQLIVGLLLDTVGELAWWVRLDAGRCKLRQEGE----GFVP---------------------ITATYGWHTFDGTLQRLFFVLTMGLVAVALNSLLWVLSLPWVAWRLVCWVSYEFANEGDDDEDEETNAPVKRGKLRESLLALGTIAACWLALLFAAALCGACIVGSFFLWFRGVWNVWEWVRWRRSEEGKFVSGETDQECPTAMPGQEHGQNWRHAWQHVAKARNLRIRHEHGSEHFHVAPLLQSTTGLDMKRLCSLAKKK 392          

BLAST of mRNA_H-canaliculatus_F_contig1227.1946.1 vs. uniprot
Match: D7FLB1_ECTSI (Ankyrin Repeat Transient Receptor Potential Channel n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLB1_ECTSI)

HSP 1 Score: 157 bits (397), Expect = 1.380e-34
Identity = 190/693 (27.42%), Postives = 276/693 (39.83%), Query Frame = 2

Query: 1772 NDWTTQRLQR--LVQAAA------AVGAFDLFRVSPFYHEAIPCLLDLIGDYEENVISAVML-AMKCSRNPFVMCAVASRHIEKAESELPM-HRIGLRRLQAEVDKLGLKILEELPHTIRGVGKAFGAHEAALFKLRTNPDMQDKYNRLATKMGFIVTQWMLEPSTVLKRLSARGSRNWGDGERDDDYARFAWLPSWNMDFIDPLHRALDRGSGALDFVNCPLLLDYMSEKFSRSVPPWTSGTPFSHNV--SEHFYTHTIRD-----------YSEQHEGDARHELTSAD--LLMRFLQGWGEGRNDISSDGKNDQIVR-------------WFGVPHLTLLPGLQFTLAGIIGVPTRFYEVPAIRWFYGVLSYIAMLCLFVASVRIESTTAVPIVEKIFYIFV-AGLLWRETLEFRDFLPTHRVLRSNQDQDSFVPGSTQTGRISTTLYRYLFEDAWNILDASTILLVASAFVLRVFDLHRLSIETKPGANDNGNDNGNNLVHVGFLGQVLLAGSAIPLFSRVLSLSQIDGTLGPMTQIIWRMMSHLVKFSAVVAVLMASFAMTFHVIFCKCQETSALHEQFNTVGDAYITMFKAMLGTFDFDSFRNIEGCALPLEAEAVGITLLVLYLAMMSILLLNLLIAVLSTAHAEVHANAEKEFHLARAKLIQQSWLDVQRGRQPPPINLIMPGLGL 3733
            +DWTT  L++    Q A       A+GA       P   +    L+ L  +     +    L  + CS NPFV     S    K+ S  P   R  L  LQ  VD L L+ILE LP T++G         A                             + EP   L R  ARG                  LP        PL  AL        F   PLL D++S +F+  +P    G   + N+       T+  R             +   EG  + +  S D  LL+      G+    + S    D ++              W     LT LPG QFT+AG++G+P ++Y VPA+R    +  Y+ ML  F   V       +   E  F  ++ AG++       RDF                             LY     D WNILD   + LV    ++R  D                 DN          G+ L A SA  +FSR+L  +Q+    GPM Q+++ M + LVKF  V+ V+M  FAM+FH +F             +T G   +T+FKAMLG  DF  F +  G     + ++V   L V+YL +++++LLNLLIAVLST+H++V   A++EF +++A++I+   + V     PPP NL+   L L
Sbjct:  506 SDWTTVVLEKSNFAQGAGGGGLDWAIGALSCLLQYPTMFDTAD-LIQLSRERSAEWLRTCFLETVACSDNPFVPGMTLSIAFAKSASVAPEGERRKLLSLQERVDTLLLEILERLPQTVQGFDGGMTGCSA-----------------------------VFEPE--LSREDARG------------------LPG-------PLRLALQERQHVQTFCTQPLLTDFLSRRFTYGLP----GLRDTQNILGDRRELTNLARGGPGELMSGQKYLALDEEGRRKEDGRSLDRCLLLDLQDKIGQEGKLLDSRKIIDTLLSPCILLQGANCAGLWNTAVSLTFLPGAQFTIAGLVGMPDQYYRVPAMRMLLDLAVYLGMLVFFSTVVLFHEDGPLTPGEAAFAFYILAGIITEGQEMRRDF----------------------------GLYA---ADHWNILDLLGLGLVTGGLIVRCAD----------------GDNS--------WGRALYALSAPLIFSRLLFFAQMLRFHGPMIQVVFSMTAELVKFGVVIIVVMLGFAMSFHALFGDV----------DTFGGTCLTLFKAMLGEVDF--FGSFPGN----QYDSVATALFVIYLVVIAVMLLNLLIAVLSTSHSKVEGKADQEFKVSKARIIEHYRMVVDMCLLPPPFNLVQLILSL 1066