mRNA_H-canaliculatus_M_contigs75991.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_M_contigs75991.1.1
Unique NamemRNA_H-canaliculatus_M_contigs75991.1.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)
Homology
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: 5-oxoprolinase ((ATP-hydrolyzing) n=2 Tax=unclassified Rhodobacteraceae TaxID=58840 RepID=B6AVV9_9RHOB)

HSP 1 Score: 342 bits (878), Expect = 1.790e-113
Identity = 171/176 (97.16%), Postives = 175/176 (99.43%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKV+VSVEGDKIVSDFTGSSG+DKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVF+AFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPA ARR+EVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  249 TLKVRVSVEGDKIVSDFTGSSGLDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFNAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPANARRNEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 424
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Acetophenone (carboxylase delta subunit n=8 Tax=Rhodobacterales TaxID=204455 RepID=A0A0N7M9P8_9RHOB)

HSP 1 Score: 337 bits (865), Expect = 1.680e-111
Identity = 168/176 (95.45%), Postives = 172/176 (97.73%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKVKVSVEGD+IVSDFTG+SGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPEN+IVNALHPAPVALRHIVGHFVPD V+DAFDKIVP LVPAEGAGCLCNFQVSLRPRTDAPAP  ARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  250 TLKVKVSVEGDRIVSDFTGTSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENSIVNALHPAPVALRHIVGHFVPDAVYDAFDKIVPDLVPAEGAGCLCNFQVSLRPRTDAPAPMTARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 425
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Hydantoinase (B/oxoprolinase family protein n=1 Tax=Boseongicola sp. TaxID=1886600 RepID=A0A7Y3BE93_9RHOB)

HSP 1 Score: 336 bits (861), Expect = 3.190e-111
Identity = 165/176 (93.75%), Postives = 172/176 (97.73%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKVK+S+EGD+I+SDF G+SGVDKKGINCPLVY KAYACYALKVAIAPEIPNNAASLAPFEI AP+NTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAP +ARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  223 TLKVKISIEGDRILSDFAGTSGVDKKGINCPLVYTKAYACYALKVAIAPEIPNNAASLAPFEIAAPKNTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPESARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 398
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Acetophenone (carboxylase delta subunit n=2 Tax=Roseobacteraceae TaxID=2854170 RepID=A0A238KID8_9RHOB)

HSP 1 Score: 335 bits (860), Expect = 9.500e-111
Identity = 168/176 (95.45%), Postives = 171/176 (97.16%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKVKVSVEGDKIVS+F GSSG+DKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEI APEN+IVNALHPAPVALRHIVGHFVPD VFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAP  ARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  250 TLKVKVSVEGDKIVSNFAGSSGLDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEIEAPENSIVNALHPAPVALRHIVGHFVPDAVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPENARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 425
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Acetophenone (carboxylase delta subunit n=11 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A0P1EB40_9RHOB)

HSP 1 Score: 334 bits (856), Expect = 3.800e-110
Identity = 164/176 (93.18%), Postives = 173/176 (98.30%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKVKVSVEGD+IV+DFTG+SG+DKKGINCPLVY KAYACYALKVAIAPEIPNNAASLAPFEITAPEN+IVNALHPAPVALRHIVGHFVPD V+DAFDKIVPGLVPAEGAGCLCNFQVSLRPR+DAPAP+ ARRSEVLTFNSGGSGARPEHDG+NATAFPSGVMTMPIEATEHAGP
Sbjct:  250 TLKVKVSVEGDRIVTDFTGTSGLDKKGINCPLVYTKAYACYALKVAIAPEIPNNAASLAPFEITAPENSIVNALHPAPVALRHIVGHFVPDAVYDAFDKIVPGLVPAEGAGCLCNFQVSLRPRSDAPAPSDARRSEVLTFNSGGSGARPEHDGMNATAFPSGVMTMPIEATEHAGP 425
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: N-methylhydantoinase (B n=1 Tax=Litoreibacter ponti TaxID=1510457 RepID=A0A2T6BDP7_9RHOB)

HSP 1 Score: 333 bits (853), Expect = 1.050e-109
Identity = 165/176 (93.75%), Postives = 170/176 (96.59%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKV +SVEGD+IV+DFTG+S VDKKGINCPLVY KAYACYALKVAIAPEIPNNAASLAPFE+TAPENTIVNALHPAPVALRHIVGHFVPD VFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPA ARRSEVLTFNSGGSGARPE DGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  249 TLKVNISVEGDRIVTDFTGTSAVDKKGINCPLVYTKAYACYALKVAIAPEIPNNAASLAPFEVTAPENTIVNALHPAPVALRHIVGHFVPDAVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPATARRSEVLTFNSGGSGARPEVDGLNATAFPSGVMTMPIEATEHAGP 424
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: hydantoinase (B/oxoprolinase family protein n=1 Tax=Ruegeria sp. R13_0 TaxID=2821099 RepID=UPI001ADAFF15)

HSP 1 Score: 332 bits (852), Expect = 1.520e-109
Identity = 162/176 (92.05%), Postives = 172/176 (97.73%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKV++SVEG+KIV+DF+G+SGVDKKGINCPLVY KAYACYALKVAIAPEIPNNAASLAPFE++APENTIVNALHPAPVALRHIVGHFVPD V+DAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAP  ARRSEVLTFNSGGSGARPEHDG+NATAFPSGVMTMPIEATEHAGP
Sbjct:  250 TLKVRISVEGNKIVTDFSGTSGVDKKGINCPLVYTKAYACYALKVAIAPEIPNNAASLAPFEVSAPENTIVNALHPAPVALRHIVGHFVPDAVYDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPEGARRSEVLTFNSGGSGARPEHDGMNATAFPSGVMTMPIEATEHAGP 425
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: hydantoinase (B/oxoprolinase family protein n=1 Tax=Sulfitobacter aestuariivivens TaxID=2766981 RepID=UPI00168AC278)

HSP 1 Score: 326 bits (836), Expect = 3.900e-107
Identity = 162/176 (92.05%), Postives = 168/176 (95.45%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKV VSV  D+IV+DFTG+S +DKKGINCPLVY KAYACYALKVAIAP+IPNNAASLAPFE+TAPENTIVNALHPAPVALRHIVGHFVPD VFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPA ARRSEVLTFNSGGSGARP HDGLNATAFPSGVMTMPIEATEHAGP
Sbjct:  250 TLKVSVSVHEDRIVTDFTGTSRLDKKGINCPLVYTKAYACYALKVAIAPDIPNNAASLAPFEVTAPENTIVNALHPAPVALRHIVGHFVPDAVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPADARRSEVLTFNSGGSGARPAHDGLNATAFPSGVMTMPIEATEHAGP 425
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BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Acetophenone (carboxylase delta subunit n=1 Tax=Planktotalea frisia TaxID=696762 RepID=A0A1L9P2E7_9RHOB)

HSP 1 Score: 325 bits (834), Expect = 7.810e-107
Identity = 162/176 (92.05%), Postives = 169/176 (96.02%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKVKV+V  D+IVSDFTGSS +DKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEI AP NTIVNA+HPAPVALRHIVGHFVPD V++AFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPA ARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMP+EATEHAGP
Sbjct:  250 TLKVKVTVHEDRIVSDFTGSSDLDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEIEAPVNTIVNAVHPAPVALRHIVGHFVPDAVYNAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAHARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPVEATEHAGP 425
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Match: Hydantoinase (B/oxoprolinase family protein n=1 Tax=Silicimonas sp. TaxID=2058390 RepID=A0A849GT89_9RHOB)

HSP 1 Score: 319 bits (817), Expect = 8.620e-107
Identity = 157/176 (89.20%), Postives = 166/176 (94.32%), Query Frame = 1 
Query:    1 TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPEIPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPEHDGLNATAFPSGVMTMPIEATEHAGP 528
            TLKV++ VE D+IV+DF G+S +DKKGINCPLVY KAYACYALKVAIAPEIPNNAASLAPFE+TAPENTIVNALHPAPVALRHIVGHFVPD V++AFDKIVPGLVPAEGAGCLCNFQVSLRPRTD PAP  ARRSEVLTFNSGGSGARPE DGLNATAFPSGVMTMPIEATEHAGP
Sbjct:   56 TLKVRIRVEKDRIVTDFAGTSPIDKKGINCPLVYTKAYACYALKVAIAPEIPNNAASLAPFEVTAPENTIVNALHPAPVALRHIVGHFVPDTVYNAFDKIVPGLVPAEGAGCLCNFQVSLRPRTDKPAPPDARRSEVLTFNSGGSGARPEQDGLNATAFPSGVMTMPIEATEHAGP 231
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_M_contigs75991.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
5-oxoprolinase1.790e-11397.16(ATP-hydrolyzing) n=2 Tax=unclassified Rhodobacter... [more]
Acetophenone1.680e-11195.45carboxylase delta subunit n=8 Tax=Rhodobacterales ... [more]
Hydantoinase3.190e-11193.75B/oxoprolinase family protein n=1 Tax=Boseongicola... [more]
Acetophenone9.500e-11195.45carboxylase delta subunit n=2 Tax=Roseobacteraceae... [more]
Acetophenone3.800e-11093.18carboxylase delta subunit n=11 Tax=Alphaproteobact... [more]
N-methylhydantoinase1.050e-10993.75B n=1 Tax=Litoreibacter ponti TaxID=1510457 RepID=... [more]
hydantoinase1.520e-10992.05B/oxoprolinase family protein n=1 Tax=Ruegeria sp.... [more]
hydantoinase3.900e-10792.05B/oxoprolinase family protein n=1 Tax=Sulfitobacte... [more]
Acetophenone7.810e-10792.05carboxylase delta subunit n=1 Tax=Planktotalea fri... [more]
Hydantoinase8.620e-10789.20B/oxoprolinase family protein n=1 Tax=Silicimonas ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_M_contigs75991contigH-canaliculatus_M_contigs75991:1..528 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male2022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog314270.RB2083_2175
Preferred nameapc4
PFAMsHydantoinase_B
Model size528
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC00632
KEGG koko:K01474
KEGG ReactionR03187
KEGG Pathwayko00330,ko01100,map00330,map01100
Hectar predicted targeting categoryother localisation
Exons1
Evalue2.93e-114
EggNOG OGsCOG0146@1|root,COG0146@2|Bacteria,1QU46@1224|Proteobacteria,2TVYU@28211|Alphaproteobacteria,3ZFZK@58840|unclassified Rhodobacteraceae
EC3.5.2.14
DescriptionN-methylhydantoinase B acetone carboxylase, alpha subunit
Cds size528
COG categoryEQ
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854097.6333222-CDS-H-canaliculatus_M_contigs75991:0..5281696854097.6333222-CDS-H-canaliculatus_M_contigs75991:0..528Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs75991 1..528 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_M_contigs75991.1.1prot_H-canaliculatus_M_contigs75991.1.1Hapterophycus canaliculatus Oshoro7m malepolypeptideH-canaliculatus_M_contigs75991 1..528 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_M_contigs75991.1.1

>prot_H-canaliculatus_M_contigs75991.1.1 ID=prot_H-canaliculatus_M_contigs75991.1.1|Name=mRNA_H-canaliculatus_M_contigs75991.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=176bp
TLKVKVSVEGDKIVSDFTGSSGVDKKGINCPLVYAKAYACYALKVAIAPE
IPNNAASLAPFEITAPENTIVNALHPAPVALRHIVGHFVPDVVFDAFDKI
VPGLVPAEGAGCLCNFQVSLRPRTDAPAPAAARRSEVLTFNSGGSGARPE
HDGLNATAFPSGVMTMPIEATEHAGP
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mRNA from alignment at H-canaliculatus_M_contigs75991:1..528-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_M_contigs75991.1.1 ID=mRNA_H-canaliculatus_M_contigs75991.1.1|Name=mRNA_H-canaliculatus_M_contigs75991.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=528bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75991:1..528- (Hapterophycus canaliculatus Oshoro7m male)
ACGTTAAAGGTGAAAGTGAGTGTTGAGGGCGACAAGATCGTGTCTGACTT
CACCGGGTCCTCTGGTGTCGACAAGAAGGGCATCAACTGTCCGCTGGTCT
ATGCCAAGGCTTATGCCTGCTATGCCTTGAAAGTCGCCATTGCGCCGGAA
ATCCCCAACAACGCAGCTTCGCTTGCCCCGTTTGAGATCACCGCCCCGGA
AAACACAATCGTCAATGCGCTGCATCCCGCACCTGTGGCCCTGCGCCACA
TTGTGGGCCACTTCGTGCCTGACGTGGTCTTTGACGCGTTTGATAAGATC
GTACCCGGCCTCGTGCCTGCCGAAGGGGCGGGGTGCCTGTGCAATTTCCA
GGTTTCCTTGCGCCCGCGTACCGACGCACCGGCACCCGCTGCGGCCCGCA
GATCAGAGGTGTTGACCTTCAACTCTGGTGGCTCAGGTGCGCGGCCTGAA
CATGATGGCTTGAACGCCACTGCCTTTCCATCTGGTGTGATGACCATGCC
GATCGAGGCAACAGAACACGCAGGTCCT
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Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs75991:1..528-

>mRNA_H-canaliculatus_M_contigs75991.1.1 ID=mRNA_H-canaliculatus_M_contigs75991.1.1|Name=mRNA_H-canaliculatus_M_contigs75991.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=528bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75991:1..528- (Hapterophycus canaliculatus Oshoro7m male)
ACGTTAAAGGTGAAAGTGAGTGTTGAGGGCGACAAGATCGTGTCTGACTT
CACCGGGTCCTCTGGTGTCGACAAGAAGGGCATCAACTGTCCGCTGGTCT
ATGCCAAGGCTTATGCCTGCTATGCCTTGAAAGTCGCCATTGCGCCGGAA
ATCCCCAACAACGCAGCTTCGCTTGCCCCGTTTGAGATCACCGCCCCGGA
AAACACAATCGTCAATGCGCTGCATCCCGCACCTGTGGCCCTGCGCCACA
TTGTGGGCCACTTCGTGCCTGACGTGGTCTTTGACGCGTTTGATAAGATC
GTACCCGGCCTCGTGCCTGCCGAAGGGGCGGGGTGCCTGTGCAATTTCCA
GGTTTCCTTGCGCCCGCGTACCGACGCACCGGCACCCGCTGCGGCCCGCA
GATCAGAGGTGTTGACCTTCAACTCTGGTGGCTCAGGTGCGCGGCCTGAA
CATGATGGCTTGAACGCCACTGCCTTTCCATCTGGTGTGATGACCATGCC
GATCGAGGCAACAGAACACGCAGGTCCT
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