mRNA_H-canaliculatus_M_contigs75864.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_H-canaliculatus_M_contigs75864.1.1
Unique Name	mRNA_H-canaliculatus_M_contigs75864.1.1
Type	mRNA
Organism	Hapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)

Homology

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=1 Tax=Sulfitobacter sp. TaxID=1903071 RepID=A0A3D2F8P6_9RHOB)

HSP 1 Score: 286 bits (733), Expect = 8.460e-94
Identity = 137/176 (77.84%), Postives = 155/176 (88.07%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NLYWMLA+FGMGFIGAEYALIF+NAQLPALGTKEEIGKISGSGFAFGY+GGV+SL +VL  +VEQ    TI GL+PIFGLD   REGTRATG   ALWFA+FMIPYF+WVRE  +T KRAS+PQAL++V+ +IK L+++PSL  YLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS
Sbjct:  117 NLYWMLASFGMGFIGAEYALIFVNAQLPALGTKEEIGKISGSGFAFGYLGGVVSLILVLALIVEQGNDLTIIGLEPIFGLDPEQREGTRATGIVTALWFAVFMIPYFMWVREKPSTAKRASVPQALNSVMISIKGLRNKPSLATYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 292

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: Major (facilitator superfamily MFS_1 n=3 Tax=Rhodobacteraceae TaxID=31989 RepID=B6ATS5_9RHOB)

HSP 1 Score: 276 bits (706), Expect = 6.670e-89
Identity = 131/176 (74.43%), Postives = 153/176 (86.93%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+WMLAAFG+GFIGAEYALIF NAQLP LGT+E+IGKISGSGFAFGY+GGVI+LAI L+ +VEQ  GKT+AGLDP+FGL+ + REGTRATGPFAA+WF +FMIPYFLWVREVRT + R+S  +A S VI ++K LKSR SLG +LVSSM YRD LNGLY FGGVYA LVL+WEV+
Sbjct:  121 NLFWMLAAFGIGFIGAEYALIFTNAQLPGLGTREQIGKISGSGFAFGYLGGVIALAIALLLLVEQGNGKTVAGLDPLFGLNPAAREGTRATGPFAAIWFIVFMIPYFLWVREVRTDQVRSSFSEAFSQVIASLKGLKSRRSLGYFLVSSMFYRDALNGLYAFGGVYAKLVLDWEVT 296

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: Penicillin-insensitive (murein endopeptidase n=1 Tax=Nymphon striatum TaxID=424472 RepID=A0A8J5D4B4_9CHEL)

HSP 1 Score: 273 bits (699), Expect = 2.090e-86
Identity = 131/177 (74.01%), Postives = 146/177 (82.49%), Query Frame = 1

Query:    1 GNLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            GNLYWMLAAFG+GFIG EYALIFIN+QLP LGT+EEIGKISGSGFAFGY+GGV+ L +VL FVVEQ  GKT+ G DP FGLD + REGTR TGP  ALWFA FMIPYFLWVREV T+  RAS   A+ +V+ ++K L  R SLG YLVSSMLYRDGLNGLY FGGVYA LVLNWEV+
Sbjct:   34 GNLYWMLAAFGLGFIGGEYALIFINSQLPGLGTREEIGKISGSGFAFGYLGGVVCLILVLGFVVEQGNGKTLFGFDPAFGLDPNAREGTRLTGPITALWFAAFMIPYFLWVREVHTSHLRASFSAAIGSVVASVKGLAHRKSLGFYLVSSMLYRDGLNGLYAFGGVYANLVLNWEVA 210

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=1 Tax=Planktotalea arctica TaxID=1481893 RepID=UPI000A178501)

HSP 1 Score: 268 bits (686), Expect = 6.680e-86
Identity = 127/176 (72.16%), Postives = 152/176 (86.36%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+WMLA FG+GFIGAEYALIF NAQLP LGT+E+IGKISGSGFAFGYMGGV+SLAI L+ +VEQ+ GKTIAGLDP+FGL+ + REGTR+TGPFAALWF +FMIPYFLWVREVRT + R+S  +A++ V  +++ LK R SLG +L+ SM YRD LNGLYTFGGVYA LVL+WEV+
Sbjct:  121 NLFWMLAFFGIGFIGAEYALIFTNAQLPGLGTREQIGKISGSGFAFGYMGGVLSLAIALLLLVEQSNGKTIAGLDPLFGLNPADREGTRSTGPFAALWFLLFMIPYFLWVREVRTDQIRSSFSEAVAQVTASLRGLKKRKSLGFFLLGSMFYRDALNGLYTFGGVYAKLVLDWEVT 296

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: Vacuole (effluxer Atg22 like protein n=1 Tax=Boseongicola aestuarii TaxID=1470561 RepID=A0A238J3C1_9RHOB)

HSP 1 Score: 259 bits (662), Expect = 3.410e-82
Identity = 127/176 (72.16%), Postives = 142/176 (80.68%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL W L  F +GFIGAEYALIF NAQLPALGT +EIGKISGSGFAFGY+GG+ISLAIVLVF VEQ  G+T+AGL P+FGLD  MREGTR  GPFAALWF +FM+PYFLWVREVR+ R+  S+  ALS++   IK L  RPSL  YL SSMLYRD LNGLY FGG YA LVLNWEV+
Sbjct:  117 NLMWALLFFAVGFIGAEYALIFTNAQLPALGTPQEIGKISGSGFAFGYLGGLISLAIVLVFFVEQANGRTVAGLAPVFGLDPEMREGTRFAGPFAALWFLVFMVPYFLWVREVRSERRGYSMKAALSSLGGAIKGLVKRPSLASYLASSMLYRDALNGLYGFGGTYALLVLNWEVT 292

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: Membrane (protein, putative n=11 Tax=Ruegeria TaxID=97050 RepID=Q5LWS7_RUEPO)

HSP 1 Score: 258 bits (659), Expect = 7.330e-82
Identity = 119/177 (67.23%), Postives = 144/177 (81.36%), Query Frame = 1

Query:    1 GNLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            GNL+WM+A+FG+GFIGAEYALIFINAQLP+LGTKEE+G+ISGSGFAFGY+GGV++L I+L+  VEQ  GKT+ GLDP+ GLD   REGTRA GPF ALWF IFMIPYFLWVRE   + +R S  +A++ V+ ++K L+ R SL  YL SSM YRD LNGLY FGGVYA LVL WE++
Sbjct:  116 GNLWWMMASFGIGFIGAEYALIFINAQLPSLGTKEEVGQISGSGFAFGYLGGVVALFIMLLLFVEQGNGKTLVGLDPVLGLDPDTREGTRAVGPFTALWFVIFMIPYFLWVREPAGSGRRGSFAEAVAMVVQSVKNLRHRGSLASYLASSMFYRDALNGLYGFGGVYARLVLGWEIT 292

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=1 Tax=Boseongicola sp. TaxID=1886600 RepID=A0A7Y3FCP0_9RHOB)

HSP 1 Score: 258 bits (659), Expect = 9.630e-82
Identity = 129/176 (73.30%), Postives = 141/176 (80.11%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+W L  F +GFIGAEYALIF NAQLP LGT +EIGKISGSGFAFGY+GG+ISLAIVLVF VEQ  G+TIAGL PI GLD  MREGTR  GPFAALWF IFMIPYFLWVREVR  R+  S+  ALS++  +IK L  RPSL  YL SSMLYRD LNGLY FGG YA LVLNWEV+
Sbjct:  117 NLFWALTFFAVGFIGAEYALIFTNAQLPTLGTPQEIGKISGSGFAFGYLGGLISLAIVLVFFVEQANGRTIAGLAPILGLDPEMREGTRFAGPFAALWFLIFMIPYFLWVREVRVERRGHSLRAALSSLGASIKGLARRPSLAAYLGSSMLYRDALNGLYGFGGTYALLVLNWEVT 292

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A1J5LC13_9PROT)

HSP 1 Score: 256 bits (654), Expect = 4.880e-81
Identity = 119/177 (67.23%), Postives = 151/177 (85.31%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRK-RASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+WML +FG+GFIGAE+AL+F+NAQLP+LGTKEE+G++SGSGFAFGY+GGVISL IVL+  VEQ++GKT+ GLDP+ GLDA+ +EGTRA GPF A+WFA+FM+PYFLWVRE RT R+ RAS  +A++ V+ ++K L  R S+  YLVSSMLYRD LNGLY FGGVYA LVL+W ++
Sbjct:  122 NLWWMLISFGIGFIGAEFALVFVNAQLPSLGTKEELGQVSGSGFAFGYLGGVISLFIVLLLFVEQDSGKTLIGLDPVLGLDATKKEGTRAVGPFVAIWFAVFMLPYFLWVREPRTARQGRASPGRAIAMVVASVKGLAHRRSMANYLVSSMLYRDALNGLYGFGGVYAGLVLDWSIT 298

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BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=14 Tax=Rhodobacterales TaxID=204455 RepID=A0A1B1A596_9RHOB)

HSP 1 Score: 256 bits (653), Expect = 7.080e-81
Identity = 121/177 (68.36%), Postives = 150/177 (84.75%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRK-RASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+WML +FG+GFIGAE+ALIF+NAQLP+LGTKEE+G++SGSGFAFGY+GGVI+L I+L+  VEQ++GKT+ GLDP+ GLDAS REGTRA GPF A+WFA+FMIPYF WVR+ R  R+  AS+ +A+S V+ +IK LK R SL  YLVSSMLYRD LNGLY FGGVYA LVL+W ++
Sbjct:  123 NLWWMLISFGVGFIGAEFALIFVNAQLPSLGTKEEVGQVSGSGFAFGYLGGVIALFIMLLLFVEQDSGKTLIGLDPVLGLDASAREGTRAVGPFVAIWFALFMIPYFAWVRDPRGPRRGSASLGRAVSMVVTSIKGLKHRKSLTSYLVSSMLYRDALNGLYGFGGVYAGLVLDWSIT 299

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Match: MFS (transporter n=9 Tax=Rhodobacterales TaxID=204455 RepID=A0A1B8RXZ0_9RHOB)

HSP 1 Score: 254 bits (650), Expect = 1.660e-80
Identity = 117/176 (66.48%), Postives = 146/176 (82.95%), Query Frame = 1

Query:    4 NLYWMLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVISLAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMIPYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRDGLNGLYTFGGVYAALVLNWEVS 531
            NL+WML +FG GFIGAE+ALIF+NAQLP+L +KE++G +SGSGFAFGY+GGVISL I+L+  VEQ +GKT+ GL P+FGLDA  REGTRA GPF ALWFA+FMIPYFLWVRE R T +R ++ QA++ V+ ++K L++R SL  YL SSMLYRD LNGLY FGGVYA LVL W+++
Sbjct:  117 NLWWMLISFGFGFIGAEFALIFVNAQLPSLASKEDVGNVSGSGFAFGYLGGVISLFIMLLLFVEQASGKTLIGLSPLFGLDAEAREGTRAVGPFTALWFAVFMIPYFLWVREGRATGRRGTVGQAVALVVQSMKNLRNRVSLASYLGSSMLYRDALNGLYGFGGVYAKLVLGWQIT 292

The following BLAST results are available for this feature:

BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.

BLAST of mRNA_H-canaliculatus_M_contigs75864.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
MFS	8.460e-94	77.84	transporter n=1 Tax=Sulfitobacter sp. TaxID=190307...	[more]
Major	6.670e-89	74.43	facilitator superfamily MFS_1 n=3 Tax=Rhodobactera...	[more]
Penicillin-insensitive	2.090e-86	74.01	murein endopeptidase n=1 Tax=Nymphon striatum TaxI...	[more]
MFS	6.680e-86	72.16	transporter n=1 Tax=Planktotalea arctica TaxID=148...	[more]
Vacuole	3.410e-82	72.16	effluxer Atg22 like protein n=1 Tax=Boseongicola a...	[more]
Membrane	7.330e-82	67.23	protein, putative n=11 Tax=Ruegeria TaxID=97050 Re...	[more]
MFS	9.630e-82	73.30	transporter n=1 Tax=Boseongicola sp. TaxID=1886600...	[more]
MFS	4.880e-81	67.23	transporter n=2 Tax=Alphaproteobacteria TaxID=2821...	[more]
MFS	7.080e-81	68.36	transporter n=14 Tax=Rhodobacterales TaxID=204455 ...	[more]
MFS	1.660e-80	66.48	transporter n=9 Tax=Rhodobacterales TaxID=204455 R...	[more]

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
H-canaliculatus_M_contigs75864	contig	H-canaliculatus_M_contigs75864:2..532 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90	2022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male	2022-07-08

Properties


Property Name	Value
Stop	0
Start	1
Seed ortholog	314270.RB2083_191
Preferred name	MA20_25070
PFAMs	ATG22
Model size	531
Max annot lvl	28211\|Alphaproteobacteria
KEGG ko	ko:K06902
KEGG TC	2.A.1.24,9.A.15.1
KEGG Pathway	ko04138,map04138
Hectar predicted targeting category	other localisation
Exons	1
Evalue	1.85e-86
EggNOG OGs	COG2270@1\|root,COG2270@2\|Bacteria,1MWXB@1224\|Proteobacteria,2TSCV@28211\|Alphaproteobacteria,3ZG39@58840\|unclassified Rhodobacteraceae
Description	Permeases of the major facilitator superfamily
Cds size	516
COG category	S
BRITE	ko00000,ko00001,ko02000,ko04131

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696854096.0983713-UTR-H-canaliculatus_M_contigs75864:1..16	1696854096.0983713-UTR-H-canaliculatus_M_contigs75864:1..16	Hapterophycus canaliculatus Oshoro7m male	UTR	H-canaliculatus_M_contigs75864 2..16 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696854096.1112192-CDS-H-canaliculatus_M_contigs75864:16..532	1696854096.1112192-CDS-H-canaliculatus_M_contigs75864:16..532	Hapterophycus canaliculatus Oshoro7m male	CDS	H-canaliculatus_M_contigs75864 17..532 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_H-canaliculatus_M_contigs75864.1.1	prot_H-canaliculatus_M_contigs75864.1.1	Hapterophycus canaliculatus Oshoro7m male	polypeptide	H-canaliculatus_M_contigs75864 17..532 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at H-canaliculatus_M_contigs75864:2..532+
coding sequence from alignment at H-canaliculatus_M_contigs75864:2..532+

protein sequence of mRNA_H-canaliculatus_M_contigs75864.1.1

>prot_H-canaliculatus_M_contigs75864.1.1 ID=prot_H-canaliculatus_M_contigs75864.1.1|Name=mRNA_H-canaliculatus_M_contigs75864.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=172bp
MLAAFGMGFIGAEYALIFINAQLPALGTKEEIGKISGSGFAFGYMGGVIS
LAIVLVFVVEQNTGKTIAGLDPIFGLDASMREGTRATGPFAALWFAIFMI
PYFLWVREVRTTRKRASIPQALSTVIDTIKRLKSRPSLGVYLVSSMLYRD
GLNGLYTFGGVYAALVLNWEVS

mRNA from alignment at H-canaliculatus_M_contigs75864:2..532+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_H-canaliculatus_M_contigs75864.1.1 ID=mRNA_H-canaliculatus_M_contigs75864.1.1|Name=mRNA_H-canaliculatus_M_contigs75864.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=531bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75864:2..532+ (Hapterophycus canaliculatus Oshoro7m male)
GGTAACCTGTATTGGATGCTGGCGGCCTTTGGCATGGGATTCATCGGGGC
GGAATACGCGCTGATCTTTATCAATGCGCAATTACCCGCGTTGGGCACCA
AGGAAGAGATCGGCAAAATCTCGGGCTCGGGATTTGCGTTTGGGTACATG
GGCGGCGTCATTTCGCTGGCCATCGTGCTGGTGTTTGTGGTCGAGCAGAA
CACAGGCAAAACCATTGCCGGGCTGGACCCGATATTCGGGCTTGATGCGT
CGATGCGCGAAGGCACCCGCGCGACCGGCCCCTTTGCCGCCCTGTGGTTT
GCGATCTTTATGATCCCCTATTTTTTGTGGGTGCGCGAAGTCCGCACAAC
CCGCAAACGCGCCAGCATCCCCCAAGCGCTGAGCACGGTTATCGACACCA
TCAAACGCCTGAAATCACGCCCCAGCCTTGGCGTGTATCTGGTGTCATCG
ATGCTGTACCGTGACGGGCTAAACGGGCTTTATACCTTTGGCGGCGTCTA
TGCGGCACTGGTGCTGAATTGGGAAGTCAGC

Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs75864:2..532+

>mRNA_H-canaliculatus_M_contigs75864.1.1 ID=mRNA_H-canaliculatus_M_contigs75864.1.1|Name=mRNA_H-canaliculatus_M_contigs75864.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=516bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75864:2..532+ (Hapterophycus canaliculatus Oshoro7m male)
ATGCTGGCGGCCTTTGGCATGGGATTCATCGGGGCGGAATACGCGCTGAT
CTTTATCAATGCGCAATTACCCGCGTTGGGCACCAAGGAAGAGATCGGCA
AAATCTCGGGCTCGGGATTTGCGTTTGGGTACATGGGCGGCGTCATTTCG
CTGGCCATCGTGCTGGTGTTTGTGGTCGAGCAGAACACAGGCAAAACCAT
TGCCGGGCTGGACCCGATATTCGGGCTTGATGCGTCGATGCGCGAAGGCA
CCCGCGCGACCGGCCCCTTTGCCGCCCTGTGGTTTGCGATCTTTATGATC
CCCTATTTTTTGTGGGTGCGCGAAGTCCGCACAACCCGCAAACGCGCCAG
CATCCCCCAAGCGCTGAGCACGGTTATCGACACCATCAAACGCCTGAAAT
CACGCCCCAGCCTTGGCGTGTATCTGGTGTCATCGATGCTGTACCGTGAC
GGGCTAAACGGGCTTTATACCTTTGGCGGCGTCTATGCGGCACTGGTGCT
GAATTGGGAAGTCAGC