prot_H-canaliculatus_F_contig1374.2988.1 (polypeptide) Hapterophycus canaliculatus Oshoro5f female

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: D7FS20_ECTSI (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FS20_ECTSI)

HSP 1 Score: 1691 bits (4378), Expect = 0.000e+0
Identity = 1022/1624 (62.93%), Postives = 1124/1624 (69.21%), Query Frame = 0

Query:   92 MLTSGVHMELAGSEDAFGRLLDHEWEQFSARPSDTRSTFLVCSAPGRANAKRLRELAGADSLVETLYRRKGEILCSFASLDRNTAALVAAEG---GASEGEPGTFSLEPLPHLAKLAPAVASDPSEAALLLSSEALASASGVTGAGKYSGVYVQESVG----GDRRLGALRGNPRAEKREVSAEHRARILAEAAAATEMGSAGGDRPSTLRLIQDGERGMVDAIEVTLTNHHERRSKSDTENLARRWLSLASDQAALATALKAEHFWSKGQPQEEGDRQVEDGAVGVEETQGEAGAREEXXXXXXXTDE--RAYALAAHEARKRLGWADVLSAVERGQGRPAARTNGTGAEDADAEATVGS-CRFDLARFTAAPSGKKVLFHRPHKMATAXXXXXXXXXXXXDEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDGFTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDT-TDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGEDSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADPSGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEGETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEFIKTNALPTTPDPSDWLDLVDQAAATLFTWKFGAALALGVLACCAAGCMWRSFRRGARHRSKVDRYRREITTGRGG-----TGRKLRTNS-VRPQPFPGPVRHPGELYVAGSGRFQESWRRPGPGSIGGRSMRSMRSEQSARGVLLTGAGAVVPGQSSEGTRPPTPPHEACPECGLQLTDVVQLVNHVETQHGGRATRKKD-------AGGTG---GLKVAEAKVMDDETYRNNVDRNVPVVTVAPSKNAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSPDATHG-SRARRAAPGGGTIHATAIRSSVAATAAGASR-------DGRSPDGARSTSP-GISGASXXXXXXXXXXXXXXXXXXXXXXXXXXXXSDASGPSISSSSGGDRNSLPRIRDLS--GPDRHGNKAAA--GTREGSSPLPRLLSHRRLGGGRDHEGGQTPRGSATPPVRGGVTGRPSSPNATMGGTRRGGSGGGXSRXXAHQKPRGYSAEQGXXXXXXXXXXXXXXXXXXXXXXXQEPAPGFEQPRRGVAKEDSVRSLTRTSSLDRMSALSSRAITRSA----GYKPEGEGSASGG----GAATARKATEHDEETTRSRRSIRRNASTEDVVPQGQRLTADRLRVLGSGLPENFELPPPVFSPVKASAKEPAYSPVDRERAGELDEDRPVSMTRKFFRQLS 1667
            MLTS VHMELAGSEDAFGRLLDHEW++FSARP+ TRSTF+VCSAPGRANAKRLRELAG DSLVETL+RRKGEILCSF       AALVAA+G          GTFSLEPLPHLAKLAPAVA+DPS AALLLSSEA         AG   GVY+ E  G    G R L A  G   A + EVSAE R +ILA        G  G DRP+T RLI+ GERGMVDAIE++L  HH +RSK +TE +ARRWLSLASDQ ALA AL+ EHFW         D  + DG+             E        T+E  RA  ++AHE RKRLGW  +L AV    G    + +        A  T  S C FDLARFT +PSGKKVL HRPH+M  A   XXXXX    DEG CLSALLAF SLQPE  YVTARRKTAT NLDAAWV+QS VDG+TPLWDENLQGQDEIIGVADTGLD NHC FREDDGD++EASDWDDPVTD DKRKVVQYIDFVDDFDE DGHGTHVAG++ GAQSG+ AGDD+ADGMAFQGKLAVFDFGDSDNFNALTTPDEID V+L+PAYDGGARVHSNSWDTVSTEYTALS EVDEFVYTHQN LVVFAAGNCGDT TDD IDNCSVLD+DEGTVLSPAQGKNVVAVGSSESGG   KD DTVSYFSSKGPTIDGRIKPDVVAPGDP +SA+AD +GETCE   QTGTSM+TP+VAGNGAMARQYFREGWYNTGSKNE+LGFDPS ALLKAVLINSAT M++AGVNPD+ TLDVTL +PPD HQGFGRVTMINALNINS+VGI+FEDW EI EGETID++VSLYEDAD DKDLR+TLVWTDPPASVGST ALIHDLDLVVIA+STGTEYYPNG EEADTVNNVEK+ I+DTT+GE Y IRV CN+LSE +TQ+FA IANGEFIKTNALPT  DPSDW DLVDQAA TL TWKF AAL+LGV ACCAA C  RS  +  RHRSKV+RYRREIT  RGG      GRKLR N   RPQP+PGPVR+PG LYV+GSGRFQESWRRPGPGSIGGRSMRSMRSEQSARG LLTGAGA+VPGQ +  T P +PPHEACPECGL+L DVVQLV HVETQHGGRA RKK        A G G   GLKV +A+ +++    N     VPVV VAPSKN                                       +P AT   SRARRA+       AT  ++ VA TA            + R+P   RS+SP G SG S                               S  S SS  GG R+ LPRIRDLS  G +  G+KA+   G   G SPLPRLLSHRR   GR +  G+T       P RG                  GGSG  X  XX+                                   +E APGFE   R + K +S RSLTRTSSLDRMSALSSRAITRS     G   + E  A  G    GA  AR      E    ++R IR + STED +P GQRLTAD+LR LGSGL       PPVFSPV  SAKEPAYSPV R+RAGELDE +PV +TRKFFRQLS
Sbjct:    1 MLTSSVHMELAGSEDAFGRLLDHEWDRFSARPAGTRSTFVVCSAPGRANAKRLRELAGGDSLVETLHRRKGEILCSF-------AALVAADGRDGXXXXXXQGTFSLEPLPHLAKLAPAVAADPSSAALLLSSEA---------AGATQGVYIDEGGGSRLRGRRHLRAAAGG--AGRSEVSAEQRDQILAXXXXXXXAGLPGDDRPTTQRLIEHGERGMVDAIEISLGRHHVKRSKGETEEMARRWLSLASDQPALAKALREEHFWGXXXXXXXXDGVMMDGSSSGXXXXXXXXXXEGEGRRRLSTEEEERATHVSAHEERKRLGWVSLLEAVGGRGGEGGGQGSSVXXXXXAAGGTRASYCGFDLARFTVSPSGKKVLLHRPHEMVAAANDXXXXX----DEG-CLSALLAFFSLQPEVRYVTARRKTATQNLDAAWVTQSAVDGYTPLWDENLQGQDEIIGVADTGLDLNHCQFREDDGDNIEASDWDDPVTDLDKRKVVQYIDFVDDFDEADGHGTHVAGSICGAQSGEDAGDDDADGMAFQGKLAVFDFGDSDNFNALTTPDEIDSVILEPAYDGGARVHSNSWDTVSTEYTALSNEVDEFVYTHQNMLVVFAAGNCGDTSTDDLIDNCSVLDDDEGTVLSPAQGKNVVAVGSSESGGVTGKDMDTVSYFSSKGPTIDGRIKPDVVAPGDPTWSAAADTTGETCEFGSQTGTSMSTPIVAGNGAMARQYFREGWYNTGSKNESLGFDPSGALLKAVLINSATGMSFAGVNPDSGTLDVTLTTPPDTHQGFGRVTMINALNINSTVGIFFEDWVEIREGETIDYDVSLYEDADTDKDLRITLVWTDPPASVGSTEALIHDLDLVVIAESTGTEYYPNGLEEADTVNNVEKITITDTTQGENYIIRVSCNELSEYDTQNFAVIANGEFIKTNALPTAQDPSDWADLVDQAADTLLTWKFAAALSLGVFACCAAACACRSCNKSRRHRSKVNRYRREITANRGGGGGGAPGRKLRPNPPARPQPYPGPVRNPGSLYVSGSGRFQESWRRPGPGSIGGRSMRSMRSEQSARGALLTGAGAMVPGQLASATAPASPPHEACPECGLRLPDVVQLVKHVETQHGGRANRKKPTATAVVAAAGNGPMPGLKVVKARAVNNGG--NPYGDGVPVVAVAPSKNVPGRVMTGNPYADARASAVEVEAKP--------------APSATSPTSRARRAS-------ATHRQAPVAGTAVXXXXXXXXXXXNNRTPTQVRSSSPAGASGTSSGRSGSSAKRSPGNPSVSGSGH-------SRSPTSSSSKPGGSRDGLPRIRDLSSHGKNNDGSKASGDGGGHSGKSPLPRLLSHRR--DGRGNGSGETA-SRVYRPSRGA-----------------GGSGDXXXXXXSRSSGETLGRRSAEVGKPWSKNESGSSGGRKQSRGLEETAPGFEGSGRTLLKNESTRSLTRTSSLDRMSALSSRAITRSTLPKEGMDEDDEAQAPPGRGVPGAVAAR------ERDMANQRRIRHSVSTEDHIPVGQRLTADKLRALGSGLA------PPVFSPVMPSAKEPAYSPVARKRAGELDEKKPVGITRKFFRQLS 1539          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A836CCI7_9STRA (Subtilisin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CCI7_9STRA)

HSP 1 Score: 424 bits (1091), Expect = 1.470e-124
Identity = 255/612 (41.67%), Postives = 356/612 (58.17%), Query Frame = 0

Query:  485 EGGCLSALLAFVSLQPEAHYVTARRKTATMN-LDAAW---------------VSQSGVDGFTPLWDENLQGQDEIIGVADTGLD-------RNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTID-NCSVLDEDEGTVLSPAQGKNVVAVGSSES--GGEDSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADP--SGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSK---NETLGFDPSAALLKAVLINSATAMTYAGVNP----------------------------------------------------DASTLDVT-LGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEGETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEFI 1012
            +G C +AL+A+VSLQP  + + A+ +   +N  DA W               V+QSG     P+W   + G  E+IG+AD+G+D       R HC F + DG  V+AS W+ PVTD  +RK++QYI +VD  DE++GHGTHVAGTL GA+ G +AG    DG AF+GK+ VFDFG SD  ++L+ P ++  ++L P+Y+ GARVHSNSW T S     +  E+D +V  HQ  L+V AAGNCGD ++ ++D        +  +V SPAQ KNVVAVG+S +     D  D +T+++FS++GPT+DGRIKPDVV PG    SASA+    G +C  A + GTSMATPVVA + A+ RQYFREGWYN+G +    E  GF PSAALLKAVL+NSA A+      P                                                     A +L +T L  PPD+ QG GR+ +   +N++++VG++F+DW++I EG+ +++ V+    A   + L VTLVWTDPP++ G+   ++H L + V  Q +    YPNG  +AD VNNV+KVV+  T +G  Y I V    ++ES TQ +A  A GEFI
Sbjct:  253 DGTCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQ----IDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFI 860          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: D7FS58_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS58_ECTSI)

HSP 1 Score: 406 bits (1043), Expect = 4.380e-117
Identity = 248/587 (42.25%), Postives = 344/587 (58.60%), Query Frame = 0

Query:  484 DEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDGFTPLWDENLQGQDEIIGVADTGLDRNHCHFRED-DGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDED---EGTVLSPAQGKNVVAVGSSESGGEDS---KDSDTVSYFSSKGPTID-GRIKPDVVAPGDPNYSASADPSGET-CEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEGETID--FEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTE--YYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEFIKTNALPTTPDPSDW-----LDLVDQAAATLFTWKFGAALALGVLA 1052
            D   CL  +LA++  +PE  YV    +   +N++AAW++QSG +    +W+E + G  EII +AD+GLD N C F ED   D+++ S W +PV D  KRK+VQY+ FVD  D E+GHGTHVAG+ AG  S  S G+   DG  F  KLAVFDFG+S+    L TP  +   MLQP YD GAR+ +NSW  V T YT+L ++VD+F Y +Q+ ++V AAGNCGD     I  C   + D    G++LSPA GKN+++VG+SE+    +    + D V+YFSS+GPT+D  RIKPDVVAPG   +SASA P  E+ CE+    GTSMATPVVAG  AM RQYF +GWY +G    +  F+PS AL+KA+++ S TAM     + D+S    TLGSPPD  QGFGRV + + LN+  SV ++ ED  ++  G+      E+     +  D D++VT+VW +P    GS + + +DLDL V+A    +E  +YPN     D  N VE VVI D    E + + V    ++ +    +A +  G    T + PT      W     +D+     A + T  F A LA  + A
Sbjct:  193 DNAHCLLTVLAYLVTRPEVSYVDDLPQVFELNVEAAWITQSGEETAYSMWNEGIDGSTEIIAMADSGLDVNSCFFTEDLTDDNIDCSSWSNPVFDLTKRKIVQYVGFVDCVDTENGHGTHVAGSAAGGLSNSSPGEHFGDGAGFGAKLAVFDFGNSEG--GLVTPSSLKSQMLQPPYDIGARISTNSWGAVRTTYTSLDRQVDDFAYLNQDMVIVIAAGNCGDE----ISGCQYQNADIYGSGSILSPALGKNIISVGASEASPSTAGLADNVDEVAYFSSQGPTVDDNRIKPDVVAPGYHLFSASAKPDQESSCEIGPMAGTSMATPVVAGAAAMIRQYFVQGWYPSGIATPSNIFNPSNALVKAMIVTSGTAMA----SYDSSAGSTTLGSPPDAIQGFGRVLLDSVLNVRGSVDLFVEDRVDMTHGDVNSHFIEMDGVSSSAFDGDMKVTMVWMEPAGFSGSVAPVFNDLDLKVVAYDGTSESTFYPNNLAAKDPDNTVEMVVIPDVDSYEWFNVTVTAASVTTTSLPYYALVVTGGVAGTYS-PTASTARSWFVRWEMDIDGIPVAQVATEAFEAGLAEAISA 768          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A835YK17_9STRA (Subtilisin (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YK17_9STRA)

HSP 1 Score: 363 bits (933), Expect = 1.090e-104
Identity = 237/539 (43.97%), Postives = 320/539 (59.37%), Query Frame = 0

Query:  488 CLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDGFTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDG-DSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQ--SGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGEDSK---DSDTVSYFSSKGPTI-DGRIKPDVVAPGDPNYSASADPSGE-TCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMT-YAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEG-----ETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQS--TGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSE-SETQSFAFIANG 1009
            CL +L AF+       YV        +N  AAW+ QSGV+G  P+W   LQ   EII VAD+G+D   C F E+D  D++  S + +PVTD  KRKVVQY+ +VD  DE+ GHGTHVAGTLAGAQ  +  + G    DG+AF  KLAVFDFGD+    AL TP ++   ML   Y  GARV SNSW      YT L  ++D + Y   + +VV AAGNCGD       +        G+VLSPA  KNV+AVG+SES G  +        V+YFSS+GP   DGRIKPDVVAPG   +SA A+P+   +C +A + GTSMATPVVA   AM RQYF +GWY +G+ N +   +PS AL+KAVL+N+A  MT Y G+   A+T  V LG+ PD +QG+GRV +  ALN+  SV ++ +D   +Y       +T  F V    DA    D R T+V+ +P  +VG+ S +++DLDL+V A    T + ++PNGG  AD VN VE+V + D     ++ + V  + ++  ++   FA +  G
Sbjct:   24 CLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDA--AGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGL---AAT--VPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDM--VYMNVTGMVKTYTFAV----DAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTG 549          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: K8ZC45_NANGC (Subtilisin n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=K8ZC45_NANGC)

HSP 1 Score: 340 bits (871), Expect = 3.640e-94
Identity = 221/542 (40.77%), Postives = 296/542 (54.61%), Query Frame = 0

Query:  484 DEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDG-------FTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTE-YTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGEDSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADPSGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINS-----SVGIYFEDWKEIYEGETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEFI 1012
            D+  CL ALLAFVS  P   YV         N  AA ++QSG DG         PL+D  L G ++++ V DTG+D+N C F +  G  +  +   +   D+ +RK+VQY+ + D  D E+GHGTHVAGT+AG     S      +G+A   KLA FD G S     LT P      +  P Y  GAR+ SNSW T S + YT+   E+D+F+Y + + LV+ + GN G              E  G V SPA  KNV++VG+S S      D   V+YFSS GPT DGRIKPD+V PG    SA+   SG TC +  + GTSMA PV AG+ AM RQYF EGWY  G K    GF P+ ALLKA+LINSA  M     N    TL + LG PPD +QG GR+ + + L I S     +  +Y  +   I E E   ++ S+   A     +++TLVW DP  +  S + ++HDLDL+V +Q  G+ Y+PNG   AD VN VEKV +   T G+T T+ V    ++    Q +A  A+G F+
Sbjct:  367 DQSACLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAHLTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVDQNSCFFSDAKG-PLPTTTISEAAFDSSRRKIVQYVQWADSTDVENGHGTHVAGTMAGVSLDTSMDMSSYEGVARGAKLAFFDMGFSSG--GLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKGR-------------EGIGGVASPALSKNVLSVGASLSNRYADLDPTHVAYFSSWGPTKDGRIKPDIVTPGRRLVSAA---SGATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAADGFVPTGALLKAMLINSAVPMAAFDFNN--GTL-LPLGLPPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKFSI--PATGKSPVKITLVWIDPDTTASSATTVLHDLDLMVYSQKEGSSYFPNGRTNADNVNTVEKVALYSPTPGDTLTVTVTATSVATRPEQRYALAASGNFV 884          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A4D9CUI1_9STRA (Subtilisin n=2 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CUI1_9STRA)

HSP 1 Score: 337 bits (864), Expect = 1.700e-91
Identity = 220/542 (40.59%), Postives = 295/542 (54.43%), Query Frame = 0

Query:  484 DEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDG-------FTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTE-YTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGEDSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADPSGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINS-----SVGIYFEDWKEIYEGETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEFI 1012
            D+  CL ALLAFVS  P   YV         N  AA ++QSG DG         PL+D  L G ++++ V DTG+D+N C F +  G  +  +   +   D+ +RK+VQY+ + D  D E+GHGTHVAGT+AG     S      +G+A   KLA FD G S     LT P      +  P Y  GAR+ SNSW T S + YT+   E+D+F+Y + + LV+ + GN G              E  G V SPA  KNV++VG+S S      D   V+YFSS GPT DGRIKPD+V PG    SA+   SG TC +  + GTSMA PV AG+ AM RQYF EGWY  G K    GF P+ ALLKA+LINSA  M     N    TL + LG PPD +QG GR+ + + L I S     +  +Y  +   I E E   +++S+   A     +++TLVW DP     S + ++HDLDL+V +Q  G+ Y+PNG   AD VN VEKV +     G+T T+ V    ++    Q +A  A+G F+
Sbjct:  368 DQSACLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAHLTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVDQNSCFFSDAKG-PLPTTTISEAAFDSSRRKIVQYVQWADSTDVENGHGTHVAGTVAGVSLDTSMDMSSYEGVARGAKLAFFDMGFSSG--GLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKGG-------------EGIGGVASPALSKNVLSVGASLSDRYADLDPTHVAYFSSWGPTKDGRIKPDIVTPGRRLVSAA---SGATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAADGFVPTGALLKAMLINSAVPMAAFDFNN--GTL-LPLGLPPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKLSI--PATGKSPVKITLVWIDPDTRASSATTVLHDLDLMVYSQKEGSSYFPNGRTNADNVNTVEKVALYSPMPGDTLTVTVTATSVATRPEQRYALAASGNFV 885          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A7S3YRY0_9EUKA (Subtilisin n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S3YRY0_9EUKA)

HSP 1 Score: 315 bits (807), Expect = 6.800e-87
Identity = 215/536 (40.11%), Postives = 296/536 (55.22%), Query Frame = 0

Query:  488 CLSALLAFVSLQPEAHYVTARRKTATM-NLDAAWVSQSGVDGFTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQSGDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGG-ARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGEDSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADPSG--ETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMT--YAGVNPDAS-TLDVTLGSPPDEHQGFGRVTMINALNINSSVG-----IYFEDWKEIYEGETIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVI--SDTTEGETYTIRVICNDLSESETQSFAFIANG 1009
            C  A+L F++ Q +  ++ A     T  N +A+WV QSG     PL+D  + GQ ++IG++DTG+D N C F + +G   ++        D+ KRKVV YI F D  D   GHG+H AG++AG   G +A   +A G+A   KLAV D   S N   L  P  +    L+P +D   AR+HS SW + +  YT  S + D FVY + N LV+ AAGN G                 G+V SPA  KN +AVG+S S   +   S     FSS GPT DGR+KPD++APG    SASA PSG  +TC   F++GTSMATPV AG  A+ RQY  E     G       F PSAALLKA++INSAT +   Y+G    A   +D    + P+E+QGFGRVT+ N +   ++ G     +Y ++    +     + +++   D    KDLR+TLVWTDPPA+  +   L+HDLDL V+   TGT  Y NG    DT+NNVE++ +  S  T G+TY + V    L  S+TQS++ +  G
Sbjct:  265 CGLAMLVFLAFQKDVIFIEAMDPVQTYSNSEASWVVQSGATNSVPLYDLGISGQGQVIGISDTGVDENSCFFDDPNGAVADSQVGGTYTADSSKRKVVMYIAFQDGDDCNAGHGSHCAGSIAG-NPGSTASTQDATGVARDAKLAVMDI--SQNCGGLALPTNMITGFLKPMHDTAQARIHSASWGSNTASYTYTSYQFDLFVYENPNHLVLIAAGNSGRNG-------------LGSVGSPATFKNGLAVGASCSPDTNENGSP---QFSSLGPTADGRLKPDIMAPGVSINSASAGPSGSSQTCASTFKSGTSMATPVAAGAAALLRQYLME-----GRLPRHRSFTPSAALLKAMMINSATNVNRRYSGTCTGAQQNIDYGNAATPNEYQGFGRVTLQNVVPEGTNKGAFNTYVYDDENLSSFGSRMHEVKITAPSDGSTMKDLRITLVWTDPPATSAAGKILVHDLDLYVLGP-TGTVTYANGRSSPDTLNNVERIQLLASSLTVGDTYLVNVTAKALVASQTQSYSLVITG 775          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A7S1BTB4_9STRA (Subtilisin n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1BTB4_9STRA)

HSP 1 Score: 306 bits (784), Expect = 1.880e-84
Identity = 199/540 (36.85%), Postives = 305/540 (56.48%), Query Frame = 0

Query:  488 CLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQS-GVDGFTPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGAQS------GDSAGDDEADGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTAL-SKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGED----SKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADPSGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNETLGFDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNI--NSSVGIYFEDWKEIYEGETIDFEVSLYEDADLDKD--LRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGEF 1011
            C+  +L+ +  Q E  +++ R      N++A W++Q  GV G++P +D  L G+ +I+ V+DTGLD   C+FR  D   V     ++   DT  RKVVQYI + D  D   GHGTHV G++ G         G        +G+A   K++ +D GDS    AL  P   ++V++ P YD GA+VHS SW +V+  Y +  + E DEF+Y HQ+FL++ AAGN GD   +T+ +             PA  KN+++VG++ES G       +  D ++ FSS+GPT DGRIKPDVVAPG    SASA P  E+C +  + GTSM+TP+ AGN  + RQYF EG++ +G KN   G  P+AAL+KAVL+N A      GV+    T +     P DE+QGFGR++++++L +  ++++   + D + +   +   F +++       +   L VTLVWTD  +    +  L++DLDLVV+    G +Y+PNG +  D +NN E++ + D   G+T  I +  ++L  +E Q +A    G F
Sbjct:  233 CVGVVLSVLLNQMEICHLSVRPDAVIRNMEARWITQGRGVSGYSPFYDAGLNGEGQIVAVSDTGLDTKSCYFR--DETHVVMPQLENVGVDTAHRKVVQYIAYADQTDAPQGHGTHVVGSIVGRSLVEKPLLGTLGTKLNQNGIAENAKISFYDIGDSSQ--ALYVPYPYNQVLI-PGYDAGAKVHSASWGSVNVNYYSEGAAEFDEFMYYHQDFLILVAAGNSGDAGLNTVSD-------------PATAKNIISVGATESLGSSFISGMQGMDYLASFSSRGPTKDGRIKPDVVAPGFFVASASAVPQSESCALRLEAGTSMSTPITAGNALLVRQYFEEGFHKSGKKNVENGIKPTAALVKAVLMNGA--QYILGVSNQGVTSETR---PYDENQGFGRISLLDSLPLAGHNTINGKYVDGRALQSSKNDFFNITVDNQGGKCESSILSVTLVWTDHSSRPSCSKCLLNDLDLVVVMD--GVKYHPNGRDNRDDINNAERIKL-DVKNGDTLIIGIAASNLV-TEAQHYAMAMTGCF 745          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: A0A4D9D243_9STRA (Subtilisin n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D243_9STRA)

HSP 1 Score: 314 bits (804), Expect = 2.900e-84
Identity = 219/614 (35.67%), Postives = 308/614 (50.16%), Query Frame = 0

Query:  484 DEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDGF--TPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGA-------------------------------------------------------------QSGDSAG------DDEA----DGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGE-DSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADP--SGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNE-TLG--------FDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEGE--TIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGE 1010
            D   CL  L+AF++  P+   +  R   A  N  A W+ QSG +     PLWD  L G+ E+IGVADTGLD   C F ++    V  S W+ P TD  +RKVVQY+ FVD  D  +GHGTHV+G+LAG                                                                GD  G       +EA     GMA + K+A FD   +D    L+TP +   ++  PAY+ GAR+HSNSW     EY   + ++D++ Y HQ+FL+++AAGN G+             +   ++ SP   KNV+ VG++ESG E  S+D + ++ FSS+GPT DGR+KPD+V+PG   YSA +    +G TC      GTSMA P+VAG  A+ RQYF+EG Y    +     G          PSA LLKA+ +NSA A+    +  +     V L  PPD  QG GR+ +   L+      ++F D + +  G+  T  F+++   +A     L+VTL W DPP S  +   L+HDLDL V+  S G  +YPNG    D+ NNVEK+V+ +   G  YT+ V   +LSE+E Q +A +A G+
Sbjct:   92 DRQACLEGLVAFIASHPDILSIQTRATYALQNDQATWIIQSGDEATHARPLWDLGLTGKGELIGVADTGLDDESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVEPRNEGRRGHVCAEGETLACTGMCLSAPTCQLKEYGYVSCQDWLQDGHCDAWFACPLYGCDGGDCVGLACNPNPEEALMANQGMAREAKIAFFDI--ADEHGNLSTPSDFHRMLFPPAYEAGARIHSNSWGINVGEYLFDAMKIDQYAYEHQDFLIIYAAGNYGE-------------DGFHSIGSPGNTKNVLTVGATESGPERQSQDRNYIAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGGTCSYVGMAGTSMAAPLVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDVIQPSAPLLKALFVNSAVAVKALRLEDEL----VALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGKSRTYYFQIAAASNAS-SVPLQVTLCWIDPPNSFTAQKQLLHDLDLHVLDPS-GVRFYPNGLPGPDSKNNVEKIVVVEPLPGGVYTLVVTAGELSEAEEQLYALVATGD 684          

BLAST of mRNA_H-canaliculatus_F_contig1374.2988.1 vs. uniprot
Match: W7TM23_9STRA (Subtilisin n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TM23_9STRA)

HSP 1 Score: 316 bits (809), Expect = 7.090e-84
Identity = 221/614 (35.99%), Postives = 306/614 (49.84%), Query Frame = 0

Query:  484 DEGGCLSALLAFVSLQPEAHYVTARRKTATMNLDAAWVSQSGVDGF--TPLWDENLQGQDEIIGVADTGLDRNHCHFREDDGDSVEASDWDDPVTDTDKRKVVQYIDFVDDFDEEDGHGTHVAGTLAGA-------------------------------------------------------------QSGDSAG------DDEA----DGMAFQGKLAVFDFGDSDNFNALTTPDEIDEVMLQPAYDGGARVHSNSWDTVSTEYTALSKEVDEFVYTHQNFLVVFAAGNCGDTTDDTIDNCSVLDEDEGTVLSPAQGKNVVAVGSSESGGE-DSKDSDTVSYFSSKGPTIDGRIKPDVVAPGDPNYSASADP--SGETCEMAFQTGTSMATPVVAGNGAMARQYFREGWYNTGSKNE-TLG--------FDPSAALLKAVLINSATAMTYAGVNPDASTLDVTLGSPPDEHQGFGRVTMINALNINSSVGIYFEDWKEIYEGE--TIDFEVSLYEDADLDKDLRVTLVWTDPPASVGSTSALIHDLDLVVIAQSTGTEYYPNGGEEADTVNNVEKVVISDTTEGETYTIRVICNDLSESETQSFAFIANGE 1010
            D   CL  L+AF++  P+   +  R + A  N  A W+ QSG +     PLWD  L G+ E+IGVADTGLD   C F ++    V  S W+ P TD  +RKVVQY+ FVD  D  +GHGTHV+G+LAG                                                                GD  G       +EA     GMA + K+A FD   +D    L+TP +   ++  PAY+ GAR+HSNSW     EY     ++D++ Y HQ+FL+++AAGN G+             +   ++ SP   KNV+ VG++ESG E  S+D D ++ FSS+GPT DGR+KPD+V+PG   YSA +    +G TC      GTSMA PVVAG  A+ RQYF+EG Y    +     G          PSA LLKA+ +NSA  +    +  +     V L  PPD  QG GR+ +   L+      ++F D + +  GE  T  F+++   +A     L+VTL W DPP S  +   L+HDLDL V+  S G  +YPNG    D  NNVEK+V+ +   G  YT+ V   +LSE+E Q +A +A G+
Sbjct:  419 DRQACLEGLVAFIASHPDILSIQTRARYALQNDQATWIIQSGDEATHARPLWDLGLTGKGELIGVADTGLDDESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVEPRNEGRRGHFCAEGETLACTGMCLSAPTCQLKDYGYVSCQDWLQDGHCDAWFACPLYGCDGGDCVGLACNPNPEEALMANQGMAREAKIAFFDI--ADEHGNLSTPSDFHRMLFPPAYEAGARIHSNSWGINVGEYLFDDMKIDQYAYEHQDFLIIYAAGNYGE-------------DGFHSIGSPGNTKNVLTVGATESGPERQSQDRDYIAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGGTCSYVAMAGTSMAAPVVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDVIQPSAPLLKALFVNSAVPVKALRLEDEL----VALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGESRTYYFQIAAASNAS-SVPLQVTLCWIDPPNSFTAQKQLLHDLDLHVLDPS-GVLFYPNGLPGPDRKNNVEKIVVVEPLPGGVYTLVVTAGELSEAEEQLYALVATGD 1011