mRNA_H-canaliculatus_F_contig14497.3482.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig14497.3482.1
Unique NamemRNA_H-canaliculatus_F_contig14497.3482.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A6H5L3Y1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3Y1_9PHAE)

HSP 1 Score: 109 bits (272), Expect = 1.160e-25
Identity = 67/106 (63.21%), Postives = 76/106 (71.70%), Query Frame = 1 
Query:    4 VTSALHDELAYLLMEGLLVRCAGSALER---SRGVECHSR---LDPSVFRAEHRGHIGL---PPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVR 294
            VTSALHDELAYLLMEGLLV   G+A +R   SR +   S+    D +   A +R  + L       YNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQ++
Sbjct:  308 VTSALHDELAYLLMEGLLVE-QGAAADRGQSSRRLGSSSKNTAKDKNGLAAAYRQRLQLFLQTSEKYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQLK 412
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: D7G8R8_ECTSI (CNH domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8R8_ECTSI)

HSP 1 Score: 108 bits (271), Expect = 1.630e-25
Identity = 65/106 (61.32%), Postives = 72/106 (67.92%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRL-----DPSVFRAEHRGHIGL---PPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVR 294
            EVTSALHDELAYLLMEGLLV   G+A +R               D +   A +R  + L       YNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQ++
Sbjct:  788 EVTSALHDELAYLLMEGLLVE-QGAAADRXXXXXXXXXXKNIAKDKNGLAAAYRQRLQLFLQTSEKYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQLK 892
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A835YS55_9STRA (CNH domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YS55_9STRA)

HSP 1 Score: 80.9 bits (198), Expect = 1.180e-15
Identity = 51/119 (42.86%), Postives = 63/119 (52.94%), Query Frame = 1 
Query:    7 TSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRL--------------------DPSVFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPTA 303
            T+ LHDELAYLLMEG+L       L+ +                             + S +R   R  +    R Y+ ARLL+V+PSHFL EHALLLSRLGRH+ VLHIYV Q+R  A
Sbjct:  727 TAPLHDELAYLLMEGVLAEQQQXXLDSAXXXXXSXXSXXXXSXXXXXXXXXXXXXXGEVSAYRRRLRTFLRAS-RSYSAARLLAVVPSHFLHEHALLLSRLGRHDAVLHIYVAQLRDAA 844
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A7S0GKL0_9STRA (Hypothetical protein n=2 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0GKL0_9STRA)

HSP 1 Score: 68.9 bits (167), Expect = 1.740e-11
Identity = 39/92 (42.39%), Postives = 52/92 (56.52%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRLDPSVFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHI 276
            E T  LHDELAYLL+EG++    G   +  +  E        ++R + R  +  P   Y    LLS +PS FL+EHALLL +LGRHE+ L I
Sbjct:  116 ERTVCLHDELAYLLLEGVIGERGGDQDDFDQDTELGH-----IYRMKLRRLLAWPASKYRVEHLLSSLPSSFLQEHALLLGKLGRHEDALRI 202
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A6G0XXS8_9STRA (CNH domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XXS8_9STRA)

HSP 1 Score: 68.6 bits (166), Expect = 2.470e-11
Identity = 42/102 (41.18%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRLDP-SVFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPTA 303
            E+ SALHDEL YLL + +      S  + S     H + D   V R +    +  P  MY+P RLLS  P   LEE A+LL+ +GRHEEVL +Y+  +R  A
Sbjct:  620 ELPSALHDELVYLLFDAIETERHKS--KTSLHARVHLQRDRLGVLRKQLLAFLQSPLAMYHPERLLSRTPMDLLEERAVLLANMGRHEEVLRLYLHDLRDAA 719
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: W4H9G2_9STRA (Uncharacterized protein n=11 Tax=Aphanomyces astaci TaxID=112090 RepID=W4H9G2_9STRA)

HSP 1 Score: 67.4 bits (163), Expect = 6.290e-11
Identity = 41/111 (36.94%), Postives = 59/111 (53.15%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSRG--------VECHSRLDPSV--FRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPTA 303
            E+   LHDEL YLL++   +  A ++L +           V  H + DP +   R      +  P  MY+P RLLS +P   LEE A+LL+ +GRHEEVL +Y+  +R  A
Sbjct:  644 ELPLTLHDELVYLLLDA--IDAAATSLRKPHQNKKDSTFHVRVHLQRDPELGPLRRHLLTFLQSPLAMYHPERLLSRVPVDMLEERAVLLANMGRHEEVLQLYLHDIRDAA 752
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A485L712_9STRA (Aste57867_17068 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485L712_9STRA)

HSP 1 Score: 67.0 bits (162), Expect = 8.600e-11
Identity = 40/104 (38.46%), Postives = 53/104 (50.96%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRLDPS---VFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPTA 303
            ++ S LHDEL YLL++ +     G   +R         L      V R      +  P  MY+P RLLS  P   LEE A+LL+ +GRHEEVL +YV  +R  A
Sbjct:  667 DIASTLHDELVYLLLDAI----DGQRQKRKDAFHPRVHLQKDRLGVLRKALLAFLQSPLAMYHPERLLSRTPPDMLEERAVLLANMGRHEEVLRLYVHDIRDAA 766
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A024UUQ4_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UUQ4_9STRA)

HSP 1 Score: 65.9 bits (159), Expect = 2.180e-10
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 1 
Query:    1 EVTSALHDELAYLLMEGLLVRCAGSALERSR-GVECHSRLDPSV--FRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPTA 303
            E+ S+LHDEL YLL++ +         ++    V  H + DP +   R      +  P  MY+P RLLS +    LEE A+LL+ +GRHEEVL +Y+ ++R  A
Sbjct:  622 ELPSSLHDELVYLLLDAIDTAQRNKTPKKEAFHVRVHLQRDPVLGPLRKHLLTFLQSPLAMYHPERLLSRMHVDMLEERAVLLANMGRHEEVLRLYLHEIRDAA 725
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: B8C9D8_THAPS (COP-gamma_platf domain-containing protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C9D8_THAPS)

HSP 1 Score: 65.9 bits (159), Expect = 2.230e-10
Identity = 36/88 (40.91%), Postives = 52/88 (59.09%), Query Frame = 1 
Query:   16 LHDELAYLLMEGLLVRCAGSALERSRGVECHSRLDPSVFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIY 279
            +HDEL+YLL+EG++        ER  G +       +++R + R  +  P  M    RLL+ +PS FL EHALLL R+GRHE+ L I+
Sbjct: 1085 MHDELSYLLLEGVIS-------ERGDGDDGEDSDLGAIYRYKLRRLLSWPNSMIRSERLLASLPSSFLREHALLLGRMGRHEDALRIF 1165
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BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Match: A0A7S2KHW2_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2KHW2_9STRA)

HSP 1 Score: 63.5 bits (153), Expect = 1.370e-9
Identity = 34/86 (39.53%), Postives = 49/86 (56.98%), Query Frame = 1 
Query:   19 HDELAYLLMEGLLVRCAGSALERSRGVECHSRLDPSVFRAEHRGHIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHI 276
            HDEL YLL+EG++    GS  +     +         ++++ R  +  P  MY   R+L+ +P+ FL EHALLL RLGRH+E L I
Sbjct:  175 HDELTYLLLEGVISEKKGSGRDELNLSK--------YYQSKLRRFLSWPGAMYRSERILASLPTSFLREHALLLGRLGRHDEALRI 252
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig14497.3482.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L3Y1_9PHAE1.160e-2563.21Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G8R8_ECTSI1.630e-2561.32CNH domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A835YS55_9STRA1.180e-1542.86CNH domain-containing protein n=1 Tax=Tribonema mi... [more]
A0A7S0GKL0_9STRA1.740e-1142.39Hypothetical protein n=2 Tax=Proboscia inermis Tax... [more]
A0A6G0XXS8_9STRA2.470e-1141.18CNH domain-containing protein n=1 Tax=Aphanomyces ... [more]
W4H9G2_9STRA6.290e-1136.94Uncharacterized protein n=11 Tax=Aphanomyces astac... [more]
A0A485L712_9STRA8.600e-1138.46Aste57867_17068 protein n=1 Tax=Aphanomyces stella... [more]
A0A024UUQ4_9STRA2.180e-1037.50Uncharacterized protein n=2 Tax=Aphanomyces invada... [more]
B8C9D8_THAPS2.230e-1040.91COP-gamma_platf domain-containing protein n=1 Tax=... [more]
A0A7S2KHW2_9STRA1.370e-939.53Hypothetical protein n=1 Tax=Leptocylindrus danicu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig14497contigH-canaliculatus_F_contig14497:495..1602 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score108.6
Seed ortholog evalue1.7e-21
Seed eggNOG ortholog2880.D7G8R8
Preferred nameVPS39
KEGG koko:K17267,ko:K20183
KEGG Pathwayko04138,map04138
GOsGO:0000322,GO:0000323,GO:0000324,GO:0000325,GO:0000329,GO:0003674,GO:0005085,GO:0005088,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0005794,GO:0005798,GO:0005911,GO:0006810,GO:0006886,GO:0006888,GO:0006890,GO:0006903,GO:0006906,GO:0006914,GO:0006950,GO:0006996,GO:0007033,GO:0007034,GO:0007041,GO:0007154,GO:0008104,GO:0008150,GO:0008152,GO:0008333,GO:0008593,GO:0009056,GO:0009267,GO:0009506,GO:0009605,GO:0009705,GO:0009966,GO:0009967,GO:0009987,GO:0009991,GO:0010008,GO:0010646,GO:0010647,GO:0012505,GO:0012506,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0016236,GO:0016237,GO:0017016,GO:0017112,GO:0017137,GO:0019899,GO:0022406,GO:0022411,GO:0023051,GO:0023056,GO:0030054,GO:0030117,GO:0030120,GO:0030126,GO:0030135,GO:0030137,GO:0030659,GO:0030660,GO:0030662,GO:0030663,GO:0030897,GO:0031090,GO:0031267,GO:0031338,GO:0031410,GO:0031667,GO:0031668,GO:0031669,GO:0031902,GO:0031982,GO:0032006,GO:0032008,GO:0032879,GO:0032889,GO:0032984,GO:0032991,GO:0033036,GO:0033043,GO:0033554,GO:0034058,GO:0034613,GO:0034727,GO:0035542,GO:0042144,GO:0042594,GO:0042886,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043254,GO:0043933,GO:0044087,GO:0044088,GO:0044232,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0044804,GO:0045184,GO:0046907,GO:0048193,GO:0048278,GO:0048284,GO:0048475,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0050896,GO:0051020,GO:0051049,GO:0051128,GO:0051179,GO:0051234,GO:0051640,GO:0051641,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051716,GO:0055044,GO:0060627,GO:0061024,GO:0061025,GO:0061919,GO:0065007,GO:0065009,GO:0070727,GO:0071496,GO:0071702,GO:0071705,GO:0071840,GO:0090174,GO:0097352,GO:0097576,GO:0097708,GO:0098588,GO:0098772,GO:0098796,GO:0098805,GO:0098852,GO:0098876,GO:0099022,GO:0099023,GO:0140056,GO:1902500,GO:1902531,GO:1902533,GO:1902774,GO:1903432,GO:1904263,GO:1990126,GO:1990816
EggNOG free text desc.intracellular protein transport
EggNOG OGsCOG5240@1,KOG1078@2759,KOG2063@1,KOG2063@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131,ko04147
Hectar predicted targeting categoryother localisation
Exons2
Model size303
Cds size303
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929201.307551-CDS-H-canaliculatus_F_contig14497:494..6231622929201.307551-CDS-H-canaliculatus_F_contig14497:494..623Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig14497 495..623 -
1696845715.3114178-CDS-H-canaliculatus_F_contig14497:494..6231696845715.3114178-CDS-H-canaliculatus_F_contig14497:494..623Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig14497 495..623 -
1622929201.3217335-CDS-H-canaliculatus_F_contig14497:1428..16021622929201.3217335-CDS-H-canaliculatus_F_contig14497:1428..1602Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig14497 1429..1602 -
1696845715.335053-CDS-H-canaliculatus_F_contig14497:1428..16021696845715.335053-CDS-H-canaliculatus_F_contig14497:1428..1602Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig14497 1429..1602 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig14497.3482.1prot_H-canaliculatus_F_contig14497.3482.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig14497 495..1602 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig14497.3482.1

>prot_H-canaliculatus_F_contig14497.3482.1 ID=prot_H-canaliculatus_F_contig14497.3482.1|Name=mRNA_H-canaliculatus_F_contig14497.3482.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=101bp
EVTSALHDELAYLLMEGLLVRCAGSALERSRGVECHSRLDPSVFRAEHRG
HIGLPPRMYNPARLLSVIPSHFLEEHALLLSRLGRHEEVLHIYVRQVRPT
A
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mRNA from alignment at H-canaliculatus_F_contig14497:495..1602-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig14497.3482.1 ID=mRNA_H-canaliculatus_F_contig14497.3482.1|Name=mRNA_H-canaliculatus_F_contig14497.3482.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=1108bp|location=Sequence derived from alignment at H-canaliculatus_F_contig14497:495..1602- (Hapterophycus canaliculatus Oshoro5f female)
GAGGTAACGTCGGCGCTGCATGACGAGCTGGCGTACTTGCTCATGGAGGG
CCTGCTGGTGAGATGCGCTGGGAGTGCCCTTGAACGATCACGGGGGGTTG
AATGCCACTCCCGTTTGGATCCCTCGGTTTTCCGTGCGGAACACCGTGGG
CATATCGGACTCCCCCCCCGAATGGTTTCGCGCGGAGGACAGGCTTTTGA
TGCTTCGATCGGTGCGATCTAGTGTGCAAATTGTTGTGTGCAGACTAGAC
TTCACGCGTCAGGACTGCAGACTTGTGTCCTCCGGGACGCGGAGTAGACG
GTGCGCGGCGCAAGAGGCGTGCGTCACGTGCGCTTGGCAAGTGCAAGTCG
TTTTTGAACCCGTCGGTTTGGAACGCTCGCGCAGGTGGAGCAGGGAGTGG
CGGCGGATCGAAGCCAGAGCTCAAGGAAACATCTTGCGTCCCCCAGGAAT
ACTTCCAAGGATGGAATGGGCCTAGCAGGGGCATACAGGCAAAGATTACA
GCTGTTCCTGCAGGTGATTACCGATCACTCATTGCTCGAAGAGGCGTGCG
AACGTGGCTGAGGCTTGCAGTTTGACCATTCGGCGCGTGGGTAAGCTCGT
CTTGATCATGCGGTTAGAGGAAGGCTCGATCACTTACCATGCCTATTGCT
CCGTCGTCCGGGTGTAAACCAAACTGCTGTTCTCGTCGACTTCCACCCCT
GCCCCTGGTCGATTGCGTATTTGCGTCCGCTGTGGCACGTCCTACCATAC
CGTTCTGTGTTTGATACCGTTCTTAGGAATGCCGTGGTAGTGTAGACACA
CCTCTCTCACCGGTCCCCGCTCGACCTCCTTTCCACTCACTTTGTTTCCA
CCCACTATGTCTGCCGCCTACCCTCTGCACCGTCTGCCGACTCGATCATC
AACAAAAACGCCTCATCAACAACACCAACGGCGACAACAACGACGACGAC
GACGACCGCACACTAAGACGAGCGAGAAGTACAACCCCGCCCGCTTGCTG
TCGGTTATCCCGTCGCATTTCTTGGAGGAGCACGCCTTGCTGTTGTCGAG
GCTGGGGCGGCACGAGGAAGTGCTGCACATCTACGTCAGACAGGTTAGAC
CTACGGCA
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig14497:495..1602-

>mRNA_H-canaliculatus_F_contig14497.3482.1 ID=mRNA_H-canaliculatus_F_contig14497.3482.1|Name=mRNA_H-canaliculatus_F_contig14497.3482.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=606bp|location=Sequence derived from alignment at H-canaliculatus_F_contig14497:495..1602- (Hapterophycus canaliculatus Oshoro5f female)
GAGGTAACGTCGGCGCTGCATGACGAGCTGGCGTACTTGCTCATGGAGGG
CCTGCTGGTGAGATGCGCTGGGAGTGCCCTTGAACGATCACGGGGGGTTG
AATGCCACTCCCGTTTGGATCCCTCGGTTTTCCGTGCGGAACACCGTGGG
CATATCGGACTCCCCCCCCGAATGGAGGTAACGTCGGCGCTGCATGACGA
GCTGGCGTACTTGCTCATGGAGGGCCTGCTGGTGAGATGCGCTGGGAGTG
CCCTTGAACGATCACGGGGGGTTGAATGCCACTCCCGTTTGGATCCCTCG
GTTTTCCGTGCGGAACACCGTGGGCATATCGGACTCCCCCCCCGAATGTA
CAACCCCGCCCGCTTGCTGTCGGTTATCCCGTCGCATTTCTTGGAGGAGC
ACGCCTTGCTGTTGTCGAGGCTGGGGCGGCACGAGGAAGTGCTGCACATC
TACGTCAGACAGGTTAGACCTACGGCATACAACCCCGCCCGCTTGCTGTC
GGTTATCCCGTCGCATTTCTTGGAGGAGCACGCCTTGCTGTTGTCGAGGC
TGGGGCGGCACGAGGAAGTGCTGCACATCTACGTCAGACAGGTTAGACCT
ACGGCA
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