mRNA_H-canaliculatus_F_contig13776.3010.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig13776.3010.1
Unique NamemRNA_H-canaliculatus_F_contig13776.3010.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: D8LDS0_ECTSI (Lactamase_B domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDS0_ECTSI)

HSP 1 Score: 197 bits (501), Expect = 1.580e-60
Identity = 93/103 (90.29%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:   58 KVELDAIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            +V+L+AIAQHGEGDPMRVTWIGHAT+LVQIDGLNILTDPMFS+RASASQ+VGPKRF PPA+SV+ELPRIDVVLLSHNHYDHLDT SVKAVGDGPLWVVPSGLK
Sbjct:   87 EVDLEAIAQHGEGDPMRVTWIGHATLLVQIDGLNILTDPMFSERASASQMVGPKRFRPPALSVSELPRIDVVLLSHNHYDHLDTGSVKAVGDGPLWVVPSGLK 189
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A836CLM8_9STRA (Beta-lactamase superfamily domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CLM8_9STRA)

HSP 1 Score: 139 bits (351), Expect = 3.470e-37
Identity = 64/107 (59.81%), Postives = 82/107 (76.64%), Query Frame = 1 
Query:   52 IPKVELD--AIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            +P  E+D  AI  H  GD MRVTWIGHAT+LVQ+ G+N +TDP+F++R SA Q  GPKR+ PPAM V+ELP +D VLLSHNHYDHL   +V+ +G+ PLW+VP G+K
Sbjct:   83 LPFAEVDHAAIHSHRAGDAMRVTWIGHATLLVQMGGVNFITDPIFAERCSAVQWAGPKRYTPPAMRVSELPPLDFVLLSHNHYDHLCYETVQEIGNAPLWIVPKGVK 189
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A0S8CSW9_9DELT (Lactamase_B domain-containing protein n=1 Tax=Desulfobacterales bacterium SG8_35_2 TaxID=1703401 RepID=A0A0S8CSW9_9DELT)

HSP 1 Score: 135 bits (339), Expect = 4.020e-36
Identity = 61/122 (50.00%), Postives = 83/122 (68.03%), Query Frame = 1 
Query:    1 RFLSQKAVHQFSFSLFLIPKVELDAIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            R+ S +    +  +   IP+ E D  A H     ++VTWIGHATIL+Q  G+NILTDPMFSDRAS    +GPKR+ PPA+ + +LP ID +++SH+HYDHLD  +V+ +G G  W+VP GLK
Sbjct:   45 RYFSNEEFADYEANAHKIPRTEPDLAAIHNPPSDLQVTWIGHATILIQYRGVNILTDPMFSDRASPISFLGPKRYNPPALQIKDLPPIDCIVISHSHYDHLDQKTVQQIGSGTKWLVPLGLK 166
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A2N6BVS4_9DELT (MBL fold metallo-hydrolase n=1 Tax=Desulfobulbaceae bacterium TaxID=2053307 RepID=A0A2N6BVS4_9DELT)

HSP 1 Score: 132 bits (331), Expect = 9.320e-35
Identity = 59/122 (48.36%), Postives = 84/122 (68.85%), Query Frame = 1 
Query:    1 RFLSQKAVHQFSFSLFLIPKVELDAIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            R+ S +    +  +   +P+VE D  A     D ++VTWIGHATIL+Q +G+NILTDPMFSDRAS    +GPKR+ PPA+ + +LP ID +++SH+HYDHLD  +V+ +G    W+VP GL+
Sbjct:   66 RYFSNEKFADYEANAHKVPRVETDIAAIQHPMDALQVTWIGHATILIQYNGVNILTDPMFSDRASPVSFLGPKRYNPPALQIEDLPPIDYIVISHSHYDHLDQNTVQQIGSRAKWLVPLGLQ 187
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A1Y1XX77_9FUNG (Metallo-hydrolase/oxidoreductase n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1XX77_9FUNG)

HSP 1 Score: 127 bits (320), Expect = 4.750e-33
Identity = 55/92 (59.78%), Postives = 71/92 (77.17%), Query Frame = 1 
Query:   91 EGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            EG   ++TW+GHA  L+Q+DG+NIL DP+FSDR S  Q VGPKR+ PP   + ELP+ID+V++SHNHYDHLD  +VKA+GDGP + VP G K
Sbjct:   88 EGKDFQLTWLGHACFLLQVDGINILFDPVFSDRCSPFQFVGPKRYTPPPCKIEELPKIDIVVISHNHYDHLDYNTVKALGDGPRYFVPLGNK 179
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: UPI001BD11402 (MBL fold metallo-hydrolase n=1 Tax=Variovorax sp. PCZ-1 TaxID=2835533 RepID=UPI001BD11402)

HSP 1 Score: 125 bits (315), Expect = 3.420e-32
Identity = 60/100 (60.00%), Postives = 75/100 (75.00%), Query Frame = 1 
Query:   79 AQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAV----GDGPLWVVPSGLK 366
            A  G      +TWIGHAT+LVQ+ GLNILTDP+FS+RAS     GPKR+ PP +++ +LPRID+VLLSHNHYDHLDT SVKA+    G  PL++VP G+K
Sbjct:  100 ANIGSAQAPAITWIGHATMLVQMGGLNILTDPVFSERASPFSFAGPKRYQPPGIALKDLPRIDLVLLSHNHYDHLDTASVKALNAQPGGAPLFLVPLGVK 199
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A1G6EGK3_9DELT (L-ascorbate metabolism protein UlaG, beta-lactamase superfamily n=1 Tax=Desulfonatronum thiosulfatophilum TaxID=617002 RepID=A0A1G6EGK3_9DELT)

HSP 1 Score: 125 bits (313), Expect = 5.500e-32
Identity = 61/106 (57.55%), Postives = 76/106 (71.70%), Query Frame = 1 
Query:   61 VELDAIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAV----GDGPLWVVPSGLK 366
            VE D     G       TWIGHAT+L+Q+ GLNILTDP FS+RAS  Q  GP+R+ PP +++AELPRID+VL+SHNHYDHLD  SV+A+    G  PL++VP GLK
Sbjct:   83 VEADVAFVQGNRSETAATWIGHATVLMQMGGLNILTDPHFSERASPVQWAGPQRWQPPGLNLAELPRIDLVLISHNHYDHLDLASVRALAAQEGGSPLFMVPLGLK 188
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A090D3C5_9BACT (Lactamase_B domain-containing protein n=1 Tax=Criblamydia sequanensis CRIB-18 TaxID=1437425 RepID=A0A090D3C5_9BACT)

HSP 1 Score: 123 bits (308), Expect = 8.760e-32
Identity = 56/105 (53.33%), Postives = 76/105 (72.38%), Query Frame = 1 
Query:   52 IPKVELDAIAQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAVGDGPLWVVPSGLK 366
            IP V++D  A +      +V WIGH+T+L+Q +G+NILTDP+FS+R S     GPKR   PA+S+ ELP+ID VL+SHNHYDHLD  +VK +G+ P W++P GLK
Sbjct:   36 IPAVKIDLEAIYHPIYENQVLWIGHSTLLIQCEGINILTDPVFSNRCSPLAFAGPKRLVKPALSLKELPKIDYVLISHNHYDHLDEKTVKFLGNNPKWLIPLGLK 140
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: A0A2S7JEH9_9BURK (MBL fold metallo-hydrolase n=1 Tax=Rhodoferax sp. TS-BS-61-7 TaxID=2094194 RepID=A0A2S7JEH9_9BURK)

HSP 1 Score: 123 bits (309), Expect = 2.120e-31
Identity = 59/100 (59.00%), Postives = 74/100 (74.00%), Query Frame = 1 
Query:   79 AQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAV----GDGPLWVVPSGLK 366
            A  GE     +TWIGHAT+LVQ+ GLNIL DP+FSDR S  Q  GPKR+ PP +S+ +LP ID+VL+SHNHYDHLDT SV+A+    G  PL+VVP G++
Sbjct:   86 ANKGESQVPAITWIGHATMLVQMGGLNILLDPVFSDRVSPVQFAGPKRYQPPGISLQDLPHIDLVLISHNHYDHLDTGSVRALNSQAGGPPLFVVPLGVR 185
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BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Match: UPI001BCFDE75 (MBL fold metallo-hydrolase n=1 Tax=Variovorax sp. PCZ-1 TaxID=2835533 RepID=UPI001BCFDE75)

HSP 1 Score: 123 bits (308), Expect = 2.640e-31
Identity = 60/100 (60.00%), Postives = 72/100 (72.00%), Query Frame = 1 
Query:   79 AQHGEGDPMRVTWIGHATILVQIDGLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDHLDTTSVKAV----GDGPLWVVPSGLK 366
            A  G      +TWIGHAT+LVQ+ GLNILTDP FS+RAS  Q  GPKR  PP M++ +LP ID+VLLSHNHYDHLD  SVKA+    G  PL++VP G+K
Sbjct:   81 ANRGAAQEPAITWIGHATMLVQMGGLNILTDPHFSERASPVQFAGPKRAQPPGMALKDLPHIDLVLLSHNHYDHLDAGSVKALNAQAGGAPLFIVPLGVK 180
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig13776.3010.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LDS0_ECTSI1.580e-6090.29Lactamase_B domain-containing protein n=1 Tax=Ecto... [more]
A0A836CLM8_9STRA3.470e-3759.81Beta-lactamase superfamily domain-containing prote... [more]
A0A0S8CSW9_9DELT4.020e-3650.00Lactamase_B domain-containing protein n=1 Tax=Desu... [more]
A0A2N6BVS4_9DELT9.320e-3548.36MBL fold metallo-hydrolase n=1 Tax=Desulfobulbacea... [more]
A0A1Y1XX77_9FUNG4.750e-3359.78Metallo-hydrolase/oxidoreductase n=1 Tax=Basidiobo... [more]
UPI001BD114023.420e-3260.00MBL fold metallo-hydrolase n=1 Tax=Variovorax sp. ... [more]
A0A1G6EGK3_9DELT5.500e-3257.55L-ascorbate metabolism protein UlaG, beta-lactamas... [more]
A0A090D3C5_9BACT8.760e-3253.33Lactamase_B domain-containing protein n=1 Tax=Crib... [more]
A0A2S7JEH9_9BURK2.120e-3159.00MBL fold metallo-hydrolase n=1 Tax=Rhodoferax sp. ... [more]
UPI001BCFDE752.640e-3160.00MBL fold metallo-hydrolase n=1 Tax=Variovorax sp. ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig13776contigH-canaliculatus_F_contig13776:1805..2851 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score198.7
Seed ortholog evalue1.5e-48
Seed eggNOG ortholog2880.D8LDS0
Preferred nameNAPEPLD
KEGG koko:K13985
KEGG Pathwayko04723,map04723
GOsGO:0001523,GO:0001659,GO:0001750,GO:0003674,GO:0003824,GO:0004620,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005654,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005768,GO:0005769,GO:0005794,GO:0005829,GO:0005886,GO:0005929,GO:0006066,GO:0006576,GO:0006629,GO:0006644,GO:0006650,GO:0006720,GO:0006721,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0007568,GO:0008081,GO:0008150,GO:0008152,GO:0008270,GO:0009308,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016101,GO:0016298,GO:0016787,GO:0016788,GO:0019637,GO:0019866,GO:0031090,GO:0031224,GO:0031253,GO:0031300,GO:0031301,GO:0031304,GO:0031305,GO:0031347,GO:0031349,GO:0031410,GO:0031966,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0031982,GO:0032101,GO:0032103,GO:0032501,GO:0032502,GO:0032592,GO:0034308,GO:0034641,GO:0035900,GO:0036477,GO:0042439,GO:0042578,GO:0042592,GO:0042622,GO:0042802,GO:0042995,GO:0043005,GO:0043025,GO:0043167,GO:0043169,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043603,GO:0044106,GO:0044237,GO:0044238,GO:0044255,GO:0044281,GO:0044297,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044429,GO:0044441,GO:0044444,GO:0044446,GO:0044455,GO:0044459,GO:0044463,GO:0044464,GO:0045595,GO:0045597,GO:0045598,GO:0045600,GO:0046337,GO:0046486,GO:0046872,GO:0046914,GO:0048518,GO:0048519,GO:0048521,GO:0048522,GO:0048583,GO:0048584,GO:0048871,GO:0050727,GO:0050729,GO:0050789,GO:0050793,GO:0050794,GO:0050795,GO:0050896,GO:0051094,GO:0060170,GO:0060259,GO:0065007,GO:0065008,GO:0070013,GO:0070290,GO:0070291,GO:0070292,GO:0071704,GO:0071944,GO:0080134,GO:0090335,GO:0090336,GO:0097458,GO:0097708,GO:0097730,GO:0097731,GO:0097733,GO:0098573,GO:0098588,GO:0098590,GO:0120025,GO:0120038,GO:1901564,GO:1901615,GO:1903998,GO:1903999,GO:2000252
EggNOG free text desc.N-acetylphosphatidylethanolamine-hydrolysing phospholipas activity
EggNOG OGsCOG2220@1,KOG3798@2759
EC3.1.4.54
COG Functional cat.V
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionN-acyl-phosphatidylethanolamine-hydrolysing phospholipase D
Ec32 orthologEc-08_003110.1
Exons2
Model size372
Cds size372
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929119.4755461-CDS-H-canaliculatus_F_contig13776:1804..19481622929119.4755461-CDS-H-canaliculatus_F_contig13776:1804..1948Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13776 1805..1948 +
1696845685.550074-CDS-H-canaliculatus_F_contig13776:1804..19481696845685.550074-CDS-H-canaliculatus_F_contig13776:1804..1948Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13776 1805..1948 +
1622929119.5213094-CDS-H-canaliculatus_F_contig13776:2623..28511622929119.5213094-CDS-H-canaliculatus_F_contig13776:2623..2851Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13776 2624..2851 +
1696845685.570697-CDS-H-canaliculatus_F_contig13776:2623..28511696845685.570697-CDS-H-canaliculatus_F_contig13776:2623..2851Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13776 2624..2851 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig13776.3010.1prot_H-canaliculatus_F_contig13776.3010.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig13776 1805..2851 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig13776.3010.1

>prot_H-canaliculatus_F_contig13776.3010.1 ID=prot_H-canaliculatus_F_contig13776.3010.1|Name=mRNA_H-canaliculatus_F_contig13776.3010.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=124bp
RFLSQKAVHQFSFSLFLIPKVELDAIAQHGEGDPMRVTWIGHATILVQID
GLNILTDPMFSDRASASQLVGPKRFAPPAMSVAELPRIDVVLLSHNHYDH
LDTTSVKAVGDGPLWVVPSGLKG*
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mRNA from alignment at H-canaliculatus_F_contig13776:1805..2851+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig13776.3010.1 ID=mRNA_H-canaliculatus_F_contig13776.3010.1|Name=mRNA_H-canaliculatus_F_contig13776.3010.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=1047bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13776:1805..2851+ (Hapterophycus canaliculatus Oshoro5f female)
CGCTTTCTCTCTCAAAAGGCAGTACATCAATTTTCTTTCTCTCTCTTCCT
TATCCCAAAGGTGGAGCTGGACGCTATCGCTCAGCATGGGGAGGGCGACC
CGATGCGGGTGACTTGGATAGGGCACGCCACCATACTCGTCCAGGTGAGG
TGATGGATAATTTCTTCCTTCAGATTACGTGGGGGTGAGTATCGGGGCAC
ATCCCTCATCCTGGTGACCAAGAGGATTCATCTAGGCCAAACCAAGAAGA
AAAATATCGTCTTACGACAACCGCAAGCCCGTTTCCCGACGATGCATTTT
GCCACGCGGTTTGAGCGCTCTGACGACTGTAGCAAGCCTCTCTCTCGAGG
AAGACGATAGACGGCACGCACGTGCAAACTTTAACTTTCATACCGCTGGA
CCCCGTCGCCGAAGTCTCGTGGTGTCTCCGGATCACCGGAAGCGCGTGGC
CCCCTTCTCGTCGTGCCCAAGAAGACGATGGAATTGACAGTAAAGCACCT
CCCGCGAACCGCCACCCATGCCCCCGCGCTTGTGCGCGATCCCGGAGAAA
CAGGCCGCTTTGCAACACCCACCTTCATGGCATGATGGAGCACAGGGTCT
GAGTCTCAGTCTCGACCTCAACCTCAACCCTGACTGATGAGCATCGTGAA
CCTTCCTTGCCACCCCTTTCCAGCTCGCCCACTCGCCTGCCCTTCCCTCC
GACCCTTGTTCTCTATACGTTCCGTGTCCGGAACCAAAACCGTCTCCAAC
GACACAAACAAATGTCCCCACCACCAACACCACCACCACCATCAACGACG
ACGACGACGACGACGAAAGATCGACGGGCTGAACATCCTGACCGACCCGA
TGTTCTCGGACCGGGCGTCGGCGAGCCAGCTGGTTGGGCCCAAGCGCTTC
GCGCCGCCCGCGATGTCTGTGGCGGAGCTGCCCCGCATCGACGTGGTGCT
GCTGAGCCACAACCACTACGACCACCTAGATACGACCTCGGTGAAGGCGG
TCGGCGACGGTCCGCTCTGGGTCGTACCCTCCGGGCTCAAGGGGTGA
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig13776:1805..2851+

>mRNA_H-canaliculatus_F_contig13776.3010.1 ID=mRNA_H-canaliculatus_F_contig13776.3010.1|Name=mRNA_H-canaliculatus_F_contig13776.3010.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=744bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13776:1805..2851+ (Hapterophycus canaliculatus Oshoro5f female)
CGCTTTCTCTCTCAAAAGGCAGTACATCAATTTTCTTTCTCTCTCTTCCT
TATCCCAAAGGTGGAGCTGGACGCTATCGCTCAGCATGGGGAGGGCGACC
CGATGCGGGTGACTTGGATAGGGCACGCCACCATACTCGTCCAGCGCTTT
CTCTCTCAAAAGGCAGTACATCAATTTTCTTTCTCTCTCTTCCTTATCCC
AAAGGTGGAGCTGGACGCTATCGCTCAGCATGGGGAGGGCGACCCGATGC
GGGTGACTTGGATAGGGCACGCCACCATACTCGTCCAGATCGACGGGCTG
AACATCCTGACCGACCCGATGTTCTCGGACCGGGCGTCGGCGAGCCAGCT
GGTTGGGCCCAAGCGCTTCGCGCCGCCCGCGATGTCTGTGGCGGAGCTGC
CCCGCATCGACGTGGTGCTGCTGAGCCACAACCACTACGACCACCTAGAT
ACGACCTCGGTGAAGGCGGTCGGCGACGGTCCGCTCTGGGTCGTACCCTC
CGGGCTCAAGGGGTGAATCGACGGGCTGAACATCCTGACCGACCCGATGT
TCTCGGACCGGGCGTCGGCGAGCCAGCTGGTTGGGCCCAAGCGCTTCGCG
CCGCCCGCGATGTCTGTGGCGGAGCTGCCCCGCATCGACGTGGTGCTGCT
GAGCCACAACCACTACGACCACCTAGATACGACCTCGGTGAAGGCGGTCG
GCGACGGTCCGCTCTGGGTCGTACCCTCCGGGCTCAAGGGGTGA
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