mRNA_H-paniculata_contig14929.2933.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig14929.2933.1
Unique NamemRNA_H-paniculata_contig14929.2933.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: D8LDY9_ECTSI (Channel voltage activated chloride channel (Partial) (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDY9_ECTSI)

HSP 1 Score: 199 bits (505), Expect = 1.240e-62
Identity = 102/156 (65.38%), Postives = 117/156 (75.00%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVA---------------ASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            LPFLEDE PSVA +NDMTASQAMSRRVRCLRPLERAGAV DTL++C+HDCFPVVDD+GGD+LVGTILRKCLM+LLH+KAFGP   +GT    G+    SA                 AS  ISP +SW AME +YPRYPTIES+ LS+ ERR W+D
Sbjct:   35 LPFLEDECPSVARQNDMTASQAMSRRVRCLRPLERAGAVFDTLNACQHDCFPVVDDEGGDVLVGTILRKCLMLLLHQKAFGP---VGTELGDGQQNSRSAARQHQGRXXXXXXXXXDASLGISPPISWGAMERIYPRYPTIESIDLSDAERRMWMD 187          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A836CAG3_9STRA (Chloride channel protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CAG3_9STRA)

HSP 1 Score: 133 bits (335), Expect = 1.390e-33
Identity = 62/141 (43.97%), Postives = 90/141 (63.83%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            LPFLEDE+P++A++ ++TA Q M+  V+CLRP+ER G V D L + EHDCFPVVD Q G++L+GT+LRKCL +LL  + F P           RP               V++E +E  YPRYPTI+ + ++ ++R+ W+D
Sbjct:  486 LPFLEDELPALAAKGEVTAGQIMTTTVKCLRPIERVGYVYDALRNTEHDCFPVVDTQTGNVLMGTVLRKCLCVLLQRRHFSPNRGYSRGE---RP---------------VTFETLEKDYPRYPTIQEITIANDDRQSWLD 608          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A7R9ZRT7_9STRA (Chloride channel protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZRT7_9STRA)

HSP 1 Score: 121 bits (304), Expect = 8.020e-31
Identity = 60/141 (42.55%), Postives = 80/141 (56.74%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            +PF+E EVP +A R+++ A Q MS +V+CLRP+ERAG V   L    H  FP+VD   G  L GT  R  L  LL  +AFG  D L  +   G            R+SPLV W+ +E  YPRYPTI+ V + E ++  W+D
Sbjct:  110 IPFMEPEVPPIAERHEIVAGQVMSTKVKCLRPVERAGLVYQLLKESSHGTFPIVDAASGGTLYGTASRAMLCTLLQRRAFGSPDVLEEDDINGS-------LGPRRLSPLVQWDTIERAYPRYPTIDDVDMRESDQNCWLD 243          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: B8LCA7_THAPS (Chloride channel protein (Fragment) n=2 Tax=Thalassiosirales TaxID=33847 RepID=B8LCA7_THAPS)

HSP 1 Score: 118 bits (295), Expect = 3.930e-28
Identity = 63/145 (43.45%), Postives = 81/145 (55.86%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDD---QGGDILVGTILRKCLMILLHEKAFG-PEDSLGTNAATGRPQVVSAVAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            +PFLE EVP++A R+++ A Q MS  V+CLRP+ER G V D L + +H  FP+VD         L GT  R  L  LL  +AFG P D LG                  R+SPLV W+ +E VYPRYPTI+ V L   +R  W+D
Sbjct:  502 IPFLEPEVPTIAERHEIVAGQVMSTEVKCLRPVERVGIVYDLLKNVQHGNFPIVDTGELTSSGTLYGTASRSMLCTLLQRRAFGQPHDMLGPK----------------RLSPLVQWDTIERVYPRYPTIDDVELRPGDRNCWLD 630          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: K0RAV9_THAOC (Uncharacterized protein (Fragment) n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RAV9_THAOC)

HSP 1 Score: 105 bits (261), Expect = 4.150e-26
Identity = 53/131 (40.46%), Postives = 72/131 (54.96%), Query Frame = 1
Query:   40 RNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVA---ASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            R+++ A Q MS  V+CLRP+ER G V D L +  H  FP+VD      L GT  R  L  LL  +AFG    +       +P     VA      R+SPLV W+ +E VYPRYPTI+ ++L + +R  W+D
Sbjct:    2 RHEIVAGQVMSTEVKCLRPVERVGIVYDLLKNVSHGNFPIVDTASSGTLYGTASRTMLCTLLQRRAFGQPLEVNNGHYHPKPDGADDVAELLGPKRLSPLVQWDTLERVYPRYPTIDDIKLRQNDRNCWLD 132          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A4D9D9F8_9STRA (Chloride channel protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D9F8_9STRA)

HSP 1 Score: 111 bits (278), Expect = 8.460e-26
Identity = 58/141 (41.13%), Postives = 88/141 (62.41%), Query Frame = 1
Query:    4 PFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVAASGRI-SPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            P LE ++P  A +N M A++ MS   +  R +ER   + D+L+S EHDCFPV++ + G++LVGT+LRK L ++L  KAF       T+  T  P   S+    GR+ SPL+ W  +E +YPRYP I+ +R++ E+R+  ID
Sbjct:  740 PVLEPDMPPAARKN-MIAAEIMSASPKTFRRVERVSTIYDSLASTEHDCFPVLNAEDGNVLVGTVLRKALCVILQHKAF-------THPDTFSPLSESSHREKGRLFSPLLDWPTLEKIYPRYPNIQDIRITVEDRQCVID 872          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A7S2CPI8_9STRA (Chloride channel protein n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CPI8_9STRA)

HSP 1 Score: 105 bits (263), Expect = 7.090e-24
Identity = 60/152 (39.47%), Postives = 88/152 (57.89%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLG---------TNAATGRPQVVS-AVAASGRISPL-VSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            +PFLE ++P  A   ++TA   MS     ++P+ R GA+   LSSC+HDCFPVVD Q GDILVGTILR+ + +LL  +A+ PED++           N A  +P   +      G+ S   V W+ +  +YPR+P I  VR++ +ER   +D
Sbjct:  301 IPFLEGDLPIDALDAELTAGHVMSSGCVTMQPMMRVGALQSILSSCDHDCFPVVDPQRGDILVGTILRRTICVLLKHQAWFPEDAMHCDDDLDMSKANLAKKQPSAWAWRSQKQGKSSAFPVGWDEIGQLYPRFPEIWQVRITPQERELVVD 452          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A7S2B9U1_9STRA (Chloride channel protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2B9U1_9STRA)

HSP 1 Score: 100 bits (250), Expect = 1.940e-22
Identity = 53/143 (37.06%), Postives = 83/143 (58.04%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAF--GPEDSLGTNAATGRPQVVSAVAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            +P LE E+ +  +   +T  + MS+ V CLRP E+   +++ L  C+ +CFPVVD  G  +L GT+LRK L  LLH++A+  GP   + + +   R    S    S    P++++E +ESVYP YP+I  + +S EE   W+D
Sbjct:  194 MPMLEPELEAELAPYHLTVEKIMSKDVECLRPFEKVRILMELLRDCDFECFPVVDPSG--LLCGTMLRKHLTTLLHQRAYVPGPTPKIDSESFIKRDDESSGPPIS---PPIITYEILESVYPNYPSIHDINISGEELDYWLD 331          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A7S2YH57_9STRA (Chloride channel protein n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S2YH57_9STRA)

HSP 1 Score: 100 bits (249), Expect = 5.520e-22
Identity = 56/152 (36.84%), Postives = 84/152 (55.26%), Query Frame = 1
Query:    7 FLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAAT------GRPQVVSAVAASGRIS-------PLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            F+E E+ SV   +D+TA Q MS +V   RP+E+   ++DTL SC H  FPVVD + G +L GTI R  L +LL ++AFG  +  G N  +      G  +  SA+  +  +        PLV W  +E+ YP++P +E VR  + +R   +D
Sbjct:  319 FMESELKSVPRHHDLTARQIMSDQVVFFRPVEKVSNIIDTLHSCNHSFFPVVDPEDGGVLSGTIGRNELCVLLQQRAFGHPNEDGENGNSHHYKSGGLARQSSAMIYNNYMEYQGKHFFPLVEWNVLENAYPKFPKVEDVRFHDGDRDCLMD 470          
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Match: A0A836CM41_9STRA (Chloride channel protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CM41_9STRA)

HSP 1 Score: 96.7 bits (239), Expect = 1.440e-20
Identity = 52/150 (34.67%), Postives = 83/150 (55.33%), Query Frame = 1
Query:    1 LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDCFPVVDDQGG---------DILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSAVAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID 423
            +PFL+ + P    + ++   Q M++ V+CL+P+E    V  TL SC+H+CFPV+D +            +L+GTILRK L++LL  + F P  +   +A+  RP             P+++W  +E  YP+YP +E + LSEE+ R  ID
Sbjct:  433 IPFLQHQPPESIRQQNVRVHQIMTQEVKCLQPVETVERVYRTLRSCDHNCFPVIDGESRPFRRRYTLRSVLLGTILRKTLLVLLARRRFAPPSA--DDASMPRPL------------PVLAWGDLEGRYPKYPRMEHLDLSEEDGRCLID 568          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig14929.2933.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LDY9_ECTSI1.240e-6265.38Channel voltage activated chloride channel (Partia... [more]
A0A836CAG3_9STRA1.390e-3343.97Chloride channel protein n=1 Tax=Tribonema minus T... [more]
A0A7R9ZRT7_9STRA8.020e-3142.55Chloride channel protein n=1 Tax=Craspedostauros a... [more]
B8LCA7_THAPS3.930e-2843.45Chloride channel protein (Fragment) n=2 Tax=Thalas... [more]
K0RAV9_THAOC4.150e-2640.46Uncharacterized protein (Fragment) n=1 Tax=Thalass... [more]
A0A4D9D9F8_9STRA8.460e-2641.13Chloride channel protein n=2 Tax=Monodopsidaceae T... [more]
A0A7S2CPI8_9STRA7.090e-2439.47Chloride channel protein n=2 Tax=Dictyocha speculu... [more]
A0A7S2B9U1_9STRA1.940e-2237.06Chloride channel protein n=1 Tax=Dictyocha speculu... [more]
A0A7S2YH57_9STRA5.520e-2236.84Chloride channel protein n=1 Tax=Amphiprora paludo... [more]
A0A836CM41_9STRA1.440e-2034.67Chloride channel protein n=1 Tax=Tribonema minus T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig14929contigH-paniculata_contig14929:3243..3885 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score201.8
Seed ortholog evalue2.1e-49
Seed eggNOG ortholog2880.D8LDY9
Preferred nameCLCN7
KEGG koko:K05015,ko:K05016
KEGG TC2.A.49.3.3,2.A.49.3.4
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0003008,GO:0003674,GO:0005215,GO:0005216,GO:0005244,GO:0005247,GO:0005253,GO:0005254,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005764,GO:0005765,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0006807,GO:0006810,GO:0006811,GO:0006820,GO:0006821,GO:0006884,GO:0007039,GO:0007154,GO:0007165,GO:0007600,GO:0008150,GO:0008152,GO:0008308,GO:0008361,GO:0008509,GO:0009056,GO:0009057,GO:0009268,GO:0009605,GO:0009612,GO:0009628,GO:0009987,GO:0010008,GO:0012505,GO:0015075,GO:0015103,GO:0015108,GO:0015267,GO:0015291,GO:0015297,GO:0015318,GO:0015698,GO:0016020,GO:0016021,GO:0016043,GO:0019538,GO:0019725,GO:0022803,GO:0022804,GO:0022832,GO:0022836,GO:0022838,GO:0022839,GO:0022857,GO:0023052,GO:0030163,GO:0031090,GO:0031224,GO:0031410,GO:0031974,GO:0031981,GO:0031982,GO:0032501,GO:0032535,GO:0034220,GO:0042592,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050794,GO:0050877,GO:0050896,GO:0050954,GO:0050975,GO:0051179,GO:0051234,GO:0051716,GO:0055085,GO:0065007,GO:0065008,GO:0070013,GO:0071704,GO:0071840,GO:0090066,GO:0097708,GO:0098588,GO:0098656,GO:0098660,GO:0098661,GO:0098805,GO:0098852,GO:1901564,GO:1901565,GO:1901575,GO:1902476,GO:1905146
EggNOG free text desc.Chloride Channel
EggNOG OGsCOG0038@1,KOG0474@2759
Ec32 ortholog descriptionChloride channel, core
Ec32 orthologEc-23_003260.1
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01009,ko04040
Exons2
Model size423
Cds size423
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929194.3131487-CDS-H-paniculata_contig14929:3242..34621622929194.3131487-CDS-H-paniculata_contig14929:3242..3462Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14929 3243..3462 -
1691480765.336975-CDS-H-paniculata_contig14929:3242..34621691480765.336975-CDS-H-paniculata_contig14929:3242..3462Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14929 3243..3462 -
1622929194.3255873-CDS-H-paniculata_contig14929:3682..38851622929194.3255873-CDS-H-paniculata_contig14929:3682..3885Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14929 3683..3885 -
1691480765.3498993-CDS-H-paniculata_contig14929:3682..38851691480765.3498993-CDS-H-paniculata_contig14929:3682..3885Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14929 3683..3885 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig14929.2933.1prot_H-paniculata_contig14929.2933.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig14929 3243..3885 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig14929.2933.1

>prot_H-paniculata_contig14929.2933.1 ID=prot_H-paniculata_contig14929.2933.1|Name=mRNA_H-paniculata_contig14929.2933.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=141bp
LPFLEDEVPSVASRNDMTASQAMSRRVRCLRPLERAGAVLDTLSSCEHDC
FPVVDDQGGDILVGTILRKCLMILLHEKAFGPEDSLGTNAATGRPQVVSA
VAASGRISPLVSWEAMESVYPRYPTIESVRLSEEERRGWID
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mRNA from alignment at H-paniculata_contig14929:3243..3885-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig14929.2933.1 ID=mRNA_H-paniculata_contig14929.2933.1|Name=mRNA_H-paniculata_contig14929.2933.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=643bp|location=Sequence derived from alignment at H-paniculata_contig14929:3243..3885- (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTACCTTTTCTAGAGGATGAAGTGCCCTCGGTGGCGTCGAGAAATGACAT GACAGCATCCCAGGCCATGTCCAGGCGGGTTCGGTGCTTGAGGCCTCTTG AGCGCGCGGGCGCCGTTTTGGACACGCTGTCGTCGTGTGAACATGACTGT TTTCCCGTTGTGGATGACCAGGGAGGGGACATTCTCGTGGGCACAATCTT GCGGTGAGTTACGTGTCTCTGTGTGTTTGTGGGGGTTTGTTTTCAGCTAT ATTTCTGGGGGGGGTTTTAGTATTTTTATGCTTCGATTATTGTGTTCGTC GTGTGTGGGTGTGTTTTGACCGATATATTTGTCAGGGTTACGCATGTATT TTGTCTGTACACGGGCTCCGAGCCATGGCTTCACGCCGCCCTCCCTCTGC CCCCCACGTGATAAATTGAACAGAAAATGTCTCATGATCCTCTTGCACGA GAAGGCGTTTGGGCCCGAAGACTCTCTGGGTACTAATGCTGCCACCGGCC GGCCGCAGGTAGTCTCGGCCGTGGCTGCATCGGGGCGTATTTCGCCTCTG GTATCCTGGGAGGCGATGGAGAGCGTGTACCCGAGGTATCCGACGATAGA GTCGGTGCGATTGTCGGAGGAGGAGAGACGGGGATGGATCGAC
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Coding sequence (CDS) from alignment at H-paniculata_contig14929:3243..3885-

>mRNA_H-paniculata_contig14929.2933.1 ID=mRNA_H-paniculata_contig14929.2933.1|Name=mRNA_H-paniculata_contig14929.2933.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=846bp|location=Sequence derived from alignment at H-paniculata_contig14929:3243..3885- (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTACCTTTTCTAGAGGATGAAGTGCCCTCGGTGGCGTCGAGAAATGACAT
GACAGCATCCCAGGCCATGTCCAGGCGGGTTCGGTGCTTGAGGCCTCTTG
AGCGCGCGGGCGCCGTTTTGGACACGCTGTCGTCGTGTGAACATGACTGT
TTTCCCGTTGTGGATGACCAGGGAGGGGACATTCTCGTGGGCACAATCTT
GCGCTACCTTTTCTAGAGGATGAAGTGCCCTCGGTGGCGTCGAGAAATGA
CATGACAGCATCCCAGGCCATGTCCAGGCGGGTTCGGTGCTTGAGGCCTC
TTGAGCGCGCGGGCGCCGTTTTGGACACGCTGTCGTCGTGTGAACATGAC
TGTTTTCCCGTTGTGGATGACCAGGGAGGGGACATTCTCGTGGGCACAAT
CTTGCGAAAATGTCTCATGATCCTCTTGCACGAGAAGGCGTTTGGGCCCG
AAGACTCTCTGGGTACTAATGCTGCCACCGGCCGGCCGCAGGTAGTCTCG
GCCGTGGCTGCATCGGGGCGTATTTCGCCTCTGGTATCCTGGGAGGCGAT
GGAGAGCGTGTACCCGAGGTATCCGACGATAGAGTCGGTGCGATTGTCGG
AGGAGGAGAGACGGGGATGGATCGACAAAATGTCTCATGATCCTCTTGCA
CGAGAAGGCGTTTGGGCCCGAAGACTCTCTGGGTACTAATGCTGCCACCG
GCCGGCCGCAGGTAGTCTCGGCCGTGGCTGCATCGGGGCGTATTTCGCCT
CTGGTATCCTGGGAGGCGATGGAGAGCGTGTACCCGAGGTATCCGACGAT
AGAGTCGGTGCGATTGTCGGAGGAGGAGAGACGGGGATGGATCGAC
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