mRNA_H-paniculata_contig14046.2472.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig14046.2472.1
Unique NamemRNA_H-paniculata_contig14046.2472.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A6H5JD73_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JD73_9PHAE)

HSP 1 Score: 136 bits (342), Expect = 1.560e-33
Identity = 73/102 (71.57%), Postives = 84/102 (82.35%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALCSAGV 307
            SAVMILFTSFTA+TSFVVFGLL  DYAVPLFF+GLVATA GQ+GTDYLI KF+RPSFI+LSI SVVALSA LMG NG+ D+ +H     G   ++ +CSAGV
Sbjct:  407 SAVMILFTSFTATTSFVVFGLLTFDYAVPLFFLGLVATAMGQYGTDYLINKFKRPSFIILSIASVVALSAFLMGINGLYDI-LH--PTAGGDEEDGICSAGV 505          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: D7FV47_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FV47_ECTSI)

HSP 1 Score: 98.2 bits (243), Expect = 1.340e-22
Identity = 51/74 (68.92%), Postives = 64/74 (86.49%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMG 223
            SAVMIL+TSFTA+TSF+VFGLL  DYA+ LF +GL ATA GQ   ++L++K++R SFIVLSIG+VVALSAV+MG
Sbjct:    7 SAVMILYTSFTATTSFMVFGLLEEDYAIALFVVGLAATAVGQVVVNHLVKKYKRTSFIVLSIGAVVALSAVMMG 80          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A836CFF2_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CFF2_9STRA)

HSP 1 Score: 99.0 bits (245), Expect = 1.420e-21
Identity = 54/101 (53.47%), Postives = 70/101 (69.31%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALCSAG 304
            SA MILFTSFTA+TSF+VFGLLR DYA  +F +GL+AT  GQ G +YL+Q++QR S I++SIG+VVALSA+LMG   +  +      P         C+AG
Sbjct:  126 SACMILFTSFTATTSFMVFGLLRRDYAQLMFVVGLLATFVGQVGVNYLVQRYQRSSLIIVSIGAVVALSALLMGGQSLYFL----AFPRQDDESGGFCAAG 222          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A7R9YCQ0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YCQ0_9STRA)

HSP 1 Score: 97.8 bits (242), Expect = 6.690e-21
Identity = 48/74 (64.86%), Postives = 64/74 (86.49%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMG 223
            SA MIL+TSFTA++SF+ FG+++ DYA+ LFF+GLVATA GQ G +YLI+K++R S I+LSIGSVV+LS V+MG
Sbjct:  172 SACMILYTSFTATSSFIAFGMVKYDYAIFLFFVGLVATAAGQLGVNYLIKKYKRQSLIILSIGSVVSLSTVMMG 245          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A835ZGS5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZGS5_9STRA)

HSP 1 Score: 99.0 bits (245), Expect = 4.620e-20
Identity = 54/101 (53.47%), Postives = 70/101 (69.31%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALCSAG 304
            SA MILFTSFTA+TSF+VFGLLR DYA  +F +GL+AT  GQ G +YL+Q++QR S I++SIG+VVALSA+LMG   +  +      P         C+AG
Sbjct:  426 SACMILFTSFTATTSFMVFGLLRRDYAQLMFVVGLLATFVGQVGVNYLVQRYQRSSLIIVSIGAVVALSALLMGGQSLYFL----AFPRQDDESGGFCAAG 522          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A4D9D1X1_9STRA (Uncharacterized protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D1X1_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 1.380e-18
Identity = 51/100 (51.00%), Postives = 69/100 (69.00%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALCSA 301
            SA MILFTSFTA+TSF +F LL  DYAV LF IG + T+ GQ+G  YL++++ R S+I+ SIG+VV LSA+LMG   I+ ++   G   G  +  A+C A
Sbjct:  425 SACMILFTSFTATTSFYIFDLLIYDYAVWLFIIGFLFTSVGQYGLSYLMKRYNRNSYIIFSIGAVVGLSALLMGLQSIISLFS--GPGSGGKAGGAICDA 522          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A7S0AWA8_9STRA (Hypothetical protein n=1 Tax=Minutocellus polymorphus TaxID=265543 RepID=A0A7S0AWA8_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 2.630e-18
Identity = 54/98 (55.10%), Postives = 62/98 (63.27%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALC 295
            SA MILFTSFTA+TSFVVFGLL  DYA+  F IG +AT  GQ G  YL++KFQR S+I  SIG VV LSA LM    I  +Y     P   H    +C
Sbjct:   20 SACMILFTSFTATTSFVVFGLLIPDYAMICFTIGFLATLVGQIGLFYLMKKFQRNSYIAFSIGFVVLLSAFLMT---IQSLYSMAEEPRKGHGSGGVC 114          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A7S4DAW5_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DAW5_HETAK)

HSP 1 Score: 92.0 bits (227), Expect = 1.160e-17
Identity = 46/74 (62.16%), Postives = 60/74 (81.08%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMG 223
            SAVMIL+TSF+A+TSFVVFGLLR DY   LF +G++ TA GQ G +YL++K  R S+I++SIG+VVALS  +MG
Sbjct:  397 SAVMILYTSFSAATSFVVFGLLRYDYGFMLFVVGIICTAVGQVGVNYLVKKSGRGSYIIMSIGAVVALSTAMMG 470          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A6V1T5B1_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1T5B1_HETAK)

HSP 1 Score: 92.0 bits (227), Expect = 1.220e-17
Identity = 46/74 (62.16%), Postives = 60/74 (81.08%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMG 223
            SAVMIL+TSF+A+TSFVVFGLLR DY   LF +G++ TA GQ G +YL++K  R S+I++SIG+VVALS  +MG
Sbjct:  420 SAVMILYTSFSAATSFVVFGLLRYDYGFMLFVVGIICTAVGQVGVNYLVKKSGRGSYIIMSIGAVVALSTAMMG 493          
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Match: A0A7S3K149_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K149_9STRA)

HSP 1 Score: 91.3 bits (225), Expect = 2.060e-17
Identity = 48/104 (46.15%), Postives = 68/104 (65.38%), Query Frame = 2
Query:    2 SAVMILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQRPSFIVLSIGSVVALSAVLMGYNGILDVYVHWG---MPMGSHSDNALCSAG 304
            +A MIL TS TA+T+F+VFGLL  DY   LF++G++ATA GQ G  YLI+K  R S++  SIG+VV LS +LMG++G++ +        MP   H  + +C  G
Sbjct:  394 TACMILLTSTTAATTFIVFGLLAKDYGFHLFWVGVLATAVGQLGMSYLIKKSGRQSYVAFSIGAVVGLSTILMGFHGLISLAYQRADADMPGTPHHRSGICQVG 497          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig14046.2472.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JD73_9PHAE1.560e-3371.57Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FV47_ECTSI1.340e-2268.92Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A836CFF2_9STRA1.420e-2153.47Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7R9YCQ0_9STRA6.690e-2164.86Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A835ZGS5_9STRA4.620e-2053.47Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A4D9D1X1_9STRA1.380e-1851.00Uncharacterized protein n=2 Tax=Monodopsidaceae Ta... [more]
A0A7S0AWA8_9STRA2.630e-1855.10Hypothetical protein n=1 Tax=Minutocellus polymorp... [more]
A0A7S4DAW5_HETAK1.160e-1762.16Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A6V1T5B1_HETAK1.220e-1762.16Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S3K149_9STRA2.060e-1746.15Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig14046contigH-paniculata_contig14046:301..2697 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score85.9
Seed ortholog evalue1.2e-14
Seed eggNOG ortholog35128.Thaps20587
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.Sulfite exporter TauE/SafE
EggNOG OGs28PZK@1,2QWNB@2759,2XE4Z@2836
COG Functional cat.S
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
Exons3
Model size749
Cds size300
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig14046.2472.1prot_H-paniculata_contig14046.2472.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig14046 311..2258 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929119.256747-UTR-H-paniculata_contig14046:300..3101622929119.256747-UTR-H-paniculata_contig14046:300..310Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14046 301..310 +
1691480738.1011014-UTR-H-paniculata_contig14046:300..3101691480738.1011014-UTR-H-paniculata_contig14046:300..310Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14046 301..310 +
1622929119.3162615-UTR-H-paniculata_contig14046:2258..26971622929119.3162615-UTR-H-paniculata_contig14046:2258..2697Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14046 2259..2697 +
1691480738.13927-UTR-H-paniculata_contig14046:2258..26971691480738.13927-UTR-H-paniculata_contig14046:2258..2697Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14046 2259..2697 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929119.269974-CDS-H-paniculata_contig14046:310..3701622929119.269974-CDS-H-paniculata_contig14046:310..370Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 311..370 +
1691480738.1122713-CDS-H-paniculata_contig14046:310..3701691480738.1122713-CDS-H-paniculata_contig14046:310..370Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 311..370 +
1622929119.285173-CDS-H-paniculata_contig14046:1114..11981622929119.285173-CDS-H-paniculata_contig14046:1114..1198Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 1115..1198 +
1691480738.1230676-CDS-H-paniculata_contig14046:1114..11981691480738.1230676-CDS-H-paniculata_contig14046:1114..1198Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 1115..1198 +
1622929119.3018014-CDS-H-paniculata_contig14046:2102..22581622929119.3018014-CDS-H-paniculata_contig14046:2102..2258Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 2103..2258 +
1691480738.1315277-CDS-H-paniculata_contig14046:2102..22581691480738.1315277-CDS-H-paniculata_contig14046:2102..2258Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14046 2103..2258 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig14046.2472.1

>prot_H-paniculata_contig14046.2472.1 ID=prot_H-paniculata_contig14046.2472.1|Name=mRNA_H-paniculata_contig14046.2472.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=100bp
MILFTSFTASTSFVVFGLLRMDYAVPLFFIGLVATAFGQWGTDYLIQKFQ
RPSFIVLSIGSVVALSAVLMGYNGILDVYVHWGMPMGSHSDNALCSAGV*
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mRNA from alignment at H-paniculata_contig14046:301..2697+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig14046.2472.1 ID=mRNA_H-paniculata_contig14046.2472.1|Name=mRNA_H-paniculata_contig14046.2472.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=2397bp|location=Sequence derived from alignment at H-paniculata_contig14046:301..2697+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATCTGCTGTCATGATCCTGTTCACCAGCTTCACGGCGAGCACCAGCTTTG TAGTGTTTGGTCTGCTCAGGGTGAGAAACCAAGCGTGTCGAAACAACCTG CCTTTTGCTTTGGATGGCGGATTTGGTCTGGTTAGCCAAGTGCCCTTTTT ACTATCATTATTGTTGTATATGTTGGATGTGGAAAGAGGACATGATGCAG ATGGTCACAGGATCGTGTCGCATGCACAACTACAGGATTGTGTAACATGC ACATCTCTATGAATCGACACTAAACCGTCTGCCAAAATAAATAGTCTTGC CCGTTGAATGGGATACGAGGTGACCCATCACCGATTCAGTGTCATGCGTT ATCAGTCGTGTTTTTATAGTCATGTGATGGAAGTCCTCTTGAATATATAT ATATATATATTTTCAACTTTGATTGCGACCAGGTAGCTTTATTCGGCAAT TTTTGTTATCTTTGTATACCGGATAGAAAAAGAACAGTCTTGTGGTCCAC ATTCATTCTTGGGATAATAGTCCCCTTGTTCAATCTTGAGATCCGTTGGG AAGATTTTAAACTCGACCTTCCATCACATGACTTGCTTTCATACGCGCGC TTTTTCTTTCATGTGTATTGCGTCCTACCCCACCACAGTCATGATCCTTG AACATGGCCTAAAGGATTGAATGTCCATCATTTTATTGCTCCTGTGAGAT CAACCTTCCCCCTCCCAAAAGCAGACATGAAAATGTGGATACTGACCAAA GCGTGAAAAATGTTCATACCCCCCCCCTCCCCGCTGTTATACCACCCATA TTTATCCTTTGCAGATGGACTACGCCGTGCCCCTATTCTTCATCGGATTG GTGGCAACGGCGTTCGGACAGTGGGGCACCGACTACCTCATCCAAAAGGT GAGTGGAGACAATATTTTTCCCAATTTGGAACCTCGTAAAATTTTGTCAC GTTCGAAGTTGTCGGGGGGGTGTTAAATATAAGAATATTCCGGGATTGAT TAAAACCATGGTCGAACTTAGTTGAAAGGTTCTTTTTGACGGTTTAATGG TTGACGTACGTGGTCGTGAGGTACGTCGAAGGGATAGAAGGAGGTGGCGT GTGTGTTTCGTAGATTTGCTTTCATAAAAAGCTTGTCGAGCAAGCTGAAA AAAAAGACACGGGGGATTTTTTCAGAGGATCCCAACAAACGTGAGCAATC CCTGAAAGCGCCCCCAGTATGGTGAAGGTTCATGAGGGAGGCACGATGTG ATCGAAACAGAATTTGCAAACTCCAAAATCGTGCGTTCTGATTGGGCAGC CAATGCCCTTGACAGTCTCGTATGAAGTCGCGTCACACCACAGTTTCAAT AACACAAAATACCCACAGAGGCCGCCTCGCCATCGGTGTCGGGCCGTGTC TGGGCTGTGCTGGGTTGAGGGGGGTATTGACTAATCCGCGGGGGAGAGCT AGGATCGGGTGTCGATCGAGGAATATGAACGGGTTAATGCCTCTGTGCAG ATACTTCGAAGGGTTGGGGGGATGGGGGGGGCGGTCAGTGCTGTCGAATG GGGTAGTATTGCGCCCAAAAGTACAGCCCAACCCGGAAGTTAGCTGTACG CCTCCTTCGATGAAACGTTTCCTTCATCAAATATCATGTTCGGTTCGTGC CTTACTCATTTGATGCATCCCATCCTAGTTTAAATATTGCAAGTAAATCG TATATTGATACGAAACACGCGCTTGATCGCCCGCCCTCCCGCACCGTGTT CCGGTCGTGAATGCTGCTGTCCCCACTTCGTGTCCGTAATCCGTTGATAC AGTTCCAGCGGCCGAGTTTCATCGTCCTTTCCATTGGCTCTGTCGTGGCT CTTAGCGCGGTGCTGATGGGCTACAACGGGATTTTGGATGTGTACGTCCA CTGGGGCATGCCGATGGGCTCGCACTCGGATAACGCCCTTTGCTCCGCCG GAGTCTAGTCCCCCTTACCCGCCACACGTTCGCCAGGGCGAGGGTTGCAC TTCACAGGATAGAGCGATTGGTTGTGGACTGCGGCTCTTGTGGTAAGCTT CGAAGCCCCCGTGGATATGTTTGTAGTGTGGTCTAAACTGGCGCAGAATA GTGGGGCCTGCGAAACATGAACATCCCATTTACGCCCGCCGTAAAAACGT GTGACATGTAAAAAAAAACGTTGGCTGTCCATCTGCTGCACGGGTGGGAT ATGTGGCTTGTCGAGGTTCGGTATCTGAAGGAAGCGGAGCGAGCGGTACA TGCATACGAGCATCGTGTATACATATATTTTTGGAGGACGCAGTAGTTAC GCCTACTGTTGCCTCCTTGATAATTTTGATTATGCCGACATGTCAAACAA ACGTTTTTTTTTGTTTATGGGTACGGTGGACTGATGAGAGATAGACG
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Coding sequence (CDS) from alignment at H-paniculata_contig14046:301..2697+

>mRNA_H-paniculata_contig14046.2472.1 ID=mRNA_H-paniculata_contig14046.2472.1|Name=mRNA_H-paniculata_contig14046.2472.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=600bp|location=Sequence derived from alignment at H-paniculata_contig14046:301..2697+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGATCCTGTTCACCAGCTTCACGGCGAGCACCAGCTTTGTAGTGTTTGG
TCTGCTCAGGATGATCCTGTTCACCAGCTTCACGGCGAGCACCAGCTTTG
TAGTGTTTGGTCTGCTCAGGATGGACTACGCCGTGCCCCTATTCTTCATC
GGATTGGTGGCAACGGCGTTCGGACAGTGGGGCACCGACTACCTCATCCA
AAAGATGGACTACGCCGTGCCCCTATTCTTCATCGGATTGGTGGCAACGG
CGTTCGGACAGTGGGGCACCGACTACCTCATCCAAAAGTTCCAGCGGCCG
AGTTTCATCGTCCTTTCCATTGGCTCTGTCGTGGCTCTTAGCGCGGTGCT
GATGGGCTACAACGGGATTTTGGATGTGTACGTCCACTGGGGCATGCCGA
TGGGCTCGCACTCGGATAACGCCCTTTGCTCCGCCGGAGTCTAGTTCCAG
CGGCCGAGTTTCATCGTCCTTTCCATTGGCTCTGTCGTGGCTCTTAGCGC
GGTGCTGATGGGCTACAACGGGATTTTGGATGTGTACGTCCACTGGGGCA
TGCCGATGGGCTCGCACTCGGATAACGCCCTTTGCTCCGCCGGAGTCTAG
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