mRNA_H-paniculata_contig76933.15702.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig76933.15702.1
Unique NamemRNA_H-paniculata_contig76933.15702.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: D1H013_ECTSI (Cytochrome oxidase 2 (Fragment) n=9 Tax=Ectocarpus TaxID=2879 RepID=D1H013_ECTSI)

HSP 1 Score: 253 bits (645), Expect = 3.730e-80
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIADEDLVIPEALGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS
Sbjct:  381 FDSYLIADEDLVIPEALGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 502
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: D1H001_9PHAE (Cytochrome oxidase 2 (Fragment) n=3 Tax=Ectocarpus fasciculatus TaxID=43935 RepID=D1H001_9PHAE)

HSP 1 Score: 251 bits (641), Expect = 1.560e-79
Identity = 120/122 (98.36%), Postives = 120/122 (98.36%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIADEDLVIPE LGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS
Sbjct:  383 FDSYLIADEDLVIPEVLGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 504
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: E6ZES9_ECTSI (Cytochrome-c oxidase n=4 Tax=Ectocarpus TaxID=2879 RepID=E6ZES9_ECTSI)

HSP 1 Score: 253 bits (645), Expect = 1.170e-75
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIADEDLVIPEALGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS
Sbjct: 1158 FDSYLIADEDLVIPEALGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 1279
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: E3Q189_9PHAE (Cytochrome oxidase 2 (Fragment) n=24 Tax=unclassified Ectocarpus TaxID=867790 RepID=E3Q189_9PHAE)

HSP 1 Score: 233 bits (594), Expect = 1.960e-75
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 342
            FDSYLIADEDLVIPEALGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT
Sbjct:  150 FDSYLIADEDLVIPEALGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 263
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: D1H019_9PHAE (Cytochrome oxidase 2 (Fragment) n=18 Tax=Ectocarpus TaxID=2879 RepID=D1H019_9PHAE)

HSP 1 Score: 231 bits (590), Expect = 8.720e-75
Identity = 112/114 (98.25%), Postives = 112/114 (98.25%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 342
            FDSYLIADEDLVIPE LGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT
Sbjct:  153 FDSYLIADEDLVIPEVLGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 266
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: D1H027_9PHAE (Cytochrome oxidase 2 (Fragment) n=1 Tax=Kuckuckia sp. Ec160 TaxID=690469 RepID=D1H027_9PHAE)

HSP 1 Score: 231 bits (590), Expect = 8.720e-75
Identity = 112/114 (98.25%), Postives = 112/114 (98.25%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 342
            FDSYLI DEDLVIPEALGTGKAGKVF LLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT
Sbjct:  153 FDSYLITDEDLVIPEALGTGKAGKVFRLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 266
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: D1H031_9PHAE (Cytochrome oxidase 2 (Fragment) n=27 Tax=Ectocarpus TaxID=2879 RepID=D1H031_9PHAE)

HSP 1 Score: 231 bits (588), Expect = 1.750e-74
Identity = 112/114 (98.25%), Postives = 112/114 (98.25%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 342
            FDSYLIADEDLVIPEALGTGKAGKVF LLEVDNRL VPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT
Sbjct:  153 FDSYLIADEDLVIPEALGTGKAGKVFRLLEVDNRLLVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLT 266
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: A0A0U1XDJ3_9PHAE (Cytochrome-c oxidase n=1 Tax=Colpomenia peregrina TaxID=27965 RepID=A0A0U1XDJ3_9PHAE)

HSP 1 Score: 246 bits (629), Expect = 1.880e-73
Identity = 115/122 (94.26%), Postives = 119/122 (97.54%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIADEDLVIPE  GTGKAGKVF LLEVDNRLFVPTNTHIR+L+TSADVLHSWAVPSLG+KVDACPGRLNQVF+FVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS
Sbjct: 1177 FDSYLIADEDLVIPEVFGTGKAGKVFRLLEVDNRLFVPTNTHIRLLVTSADVLHSWAVPSLGIKVDACPGRLNQVFLFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 1298
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: Q94YZ5_PYLLI (Cytochrome-c oxidase n=1 Tax=Pylaiella littoralis TaxID=2885 RepID=Q94YZ5_PYLLI)

HSP 1 Score: 246 bits (628), Expect = 2.490e-73
Identity = 116/122 (95.08%), Postives = 120/122 (98.36%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIADEDLVIPEA GTGKAGKVF LLEVDNRLFVPTNTHIR+L+TSADVLHSWAVPSLGVKVDACPGRLNQVF+FVKREGVFYGQCSELCGVNHGFMPIVVQAVSQD+YLTWVGKRLCS
Sbjct: 1161 FDSYLIADEDLVIPEASGTGKAGKVFRLLEVDNRLFVPTNTHIRLLVTSADVLHSWAVPSLGVKVDACPGRLNQVFIFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDDYLTWVGKRLCS 1282
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BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Match: A0A089MYN4_PETFA (Cytochrome-c oxidase n=1 Tax=Petalonia fascia TaxID=2893 RepID=A0A089MYN4_PETFA)

HSP 1 Score: 246 bits (628), Expect = 2.610e-73
Identity = 114/122 (93.44%), Postives = 120/122 (98.36%), Query Frame = 1 
Query:    1 FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSADVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 366
            FDSYLIAD+DL+IPEA GTGKAGKVF LLEVDNRLFVPTNTHIR+L+TSADVLHSWAVPSLG+KVDACPGRLNQVF+FVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS
Sbjct: 1186 FDSYLIADDDLIIPEAYGTGKAGKVFRLLEVDNRLFVPTNTHIRLLVTSADVLHSWAVPSLGIKVDACPGRLNQVFLFVKREGVFYGQCSELCGVNHGFMPIVVQAVSQDEYLTWVGKRLCS 1307
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig76933.15702.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D1H013_ECTSI3.730e-8099.18Cytochrome oxidase 2 (Fragment) n=9 Tax=Ectocarpus... [more]
D1H001_9PHAE1.560e-7998.36Cytochrome oxidase 2 (Fragment) n=3 Tax=Ectocarpus... [more]
E6ZES9_ECTSI1.170e-7599.18Cytochrome-c oxidase n=4 Tax=Ectocarpus TaxID=2879... [more]
E3Q189_9PHAE1.960e-7599.12Cytochrome oxidase 2 (Fragment) n=24 Tax=unclassif... [more]
D1H019_9PHAE8.720e-7598.25Cytochrome oxidase 2 (Fragment) n=18 Tax=Ectocarpu... [more]
D1H027_9PHAE8.720e-7598.25Cytochrome oxidase 2 (Fragment) n=1 Tax=Kuckuckia ... [more]
D1H031_9PHAE1.750e-7498.25Cytochrome oxidase 2 (Fragment) n=27 Tax=Ectocarpu... [more]
A0A0U1XDJ3_9PHAE1.880e-7394.26Cytochrome-c oxidase n=1 Tax=Colpomenia peregrina ... [more]
Q94YZ5_PYLLI2.490e-7395.08Cytochrome-c oxidase n=1 Tax=Pylaiella littoralis ... [more]
A0A089MYN4_PETFA2.610e-7393.44Cytochrome-c oxidase n=1 Tax=Petalonia fascia TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig76933contigH-paniculata_contig76933:216..584 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score172.2
Seed ortholog evalue1.5e-40
Seed eggNOG ortholog45157.CMW010CT
Preferred nameCOX2
KEGG koko:K02256,ko:K02261
KEGG TC3.D.4.11,3.D.4.7,3.D.4.8
KEGG Pathwayko00190,ko01100,ko04260,ko04714,ko04932,ko05010,ko05012,ko05016,map00190,map01100,map04260,map04714,map04932,map05010,map05012,map05016
KEGG ModuleM00154
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004129,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005746,GO:0005751,GO:0006091,GO:0006119,GO:0006123,GO:0006139,GO:0006140,GO:0006163,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006811,GO:0006812,GO:0006950,GO:0006979,GO:0007275,GO:0007589,GO:0007595,GO:0008150,GO:0008152,GO:0008324,GO:0009055,GO:0009060,GO:0009117,GO:0009123,GO:0009126,GO:0009141,GO:0009144,GO:0009150,GO:0009161,GO:0009167,GO:0009199,GO:0009205,GO:0009259,GO:0009266,GO:0009409,GO:0009628,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010310,GO:0010562,GO:0010726,GO:0010728,GO:0010729,GO:0010939,GO:0010940,GO:0010941,GO:0010942,GO:0015002,GO:0015075,GO:0015077,GO:0015078,GO:0015318,GO:0015672,GO:0015980,GO:0016020,GO:0016021,GO:0016310,GO:0016491,GO:0016675,GO:0016676,GO:0017144,GO:0019219,GO:0019220,GO:0019222,GO:0019637,GO:0019646,GO:0019693,GO:0019866,GO:0022857,GO:0022890,GO:0022900,GO:0022904,GO:0030808,GO:0030810,GO:0030879,GO:0031090,GO:0031224,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031966,GO:0031967,GO:0031975,GO:0032501,GO:0032502,GO:0032991,GO:0034220,GO:0034641,GO:0042773,GO:0042775,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044281,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0045277,GO:0045333,GO:0045935,GO:0045937,GO:0045981,GO:0046034,GO:0046483,GO:0046903,GO:0048513,GO:0048518,GO:0048522,GO:0048731,GO:0048732,GO:0048856,GO:0050789,GO:0050794,GO:0050878,GO:0050896,GO:0051171,GO:0051173,GO:0051174,GO:0051179,GO:0051193,GO:0051194,GO:0051234,GO:0055085,GO:0055086,GO:0055114,GO:0062012,GO:0062013,GO:0065007,GO:0065008,GO:0070069,GO:0070469,GO:0071704,GO:0072521,GO:0080090,GO:0098655,GO:0098660,GO:0098662,GO:0098796,GO:0098798,GO:0098800,GO:0098803,GO:1900371,GO:1900373,GO:1900542,GO:1900544,GO:1901135,GO:1901360,GO:1901564,GO:1902600,GO:1903426,GO:1903428,GO:1903578,GO:1903580,GO:2000377,GO:2000379,GO:2001169,GO:2001171
EggNOG free text desc.oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor
EggNOG OGsCOG1622@1,KOG4767@2759
EC1.9.3.1
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03029
Exons1
Model size369
Cds size369
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930952.3523223-CDS-H-paniculata_contig76933:215..5841622930952.3523223-CDS-H-paniculata_contig76933:215..584Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig76933 216..584 -
1691481525.1989682-CDS-H-paniculata_contig76933:215..5841691481525.1989682-CDS-H-paniculata_contig76933:215..584Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig76933 216..584 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig76933.15702.1prot_H-paniculata_contig76933.15702.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig76933 216..584 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig76933.15702.1

>prot_H-paniculata_contig76933.15702.1 ID=prot_H-paniculata_contig76933.15702.1|Name=mRNA_H-paniculata_contig76933.15702.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=123bp
FDSYLIADEDLVIPEALGTGKAGKVFSLLEVDNRLFVPTNTHIRMLITSA
DVLHSWAVPSLGVKVDACPGRLNQVFMFVKREGVFYGQCSELCGVNHGFM
PIVVQAVSQDEYLTWVGKRLCS*
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mRNA from alignment at H-paniculata_contig76933:216..584-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig76933.15702.1 ID=mRNA_H-paniculata_contig76933.15702.1|Name=mRNA_H-paniculata_contig76933.15702.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=369bp|location=Sequence derived from alignment at H-paniculata_contig76933:216..584- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTTGATTCATATCTTATAGCTGATGAAGATTTAGTAATTCCAGAGGCTTT
AGGTACGGGTAAAGCAGGTAAAGTTTTCAGTCTACTCGAAGTAGATAATC
GTTTATTTGTCCCAACTAATACACATATACGTATGCTTATTACTTCTGCG
GATGTTCTTCATTCGTGGGCTGTACCAAGCTTAGGCGTTAAAGTAGATGC
TTGTCCTGGTCGTTTAAATCAAGTATTTATGTTTGTTAAGCGGGAAGGTG
TTTTCTATGGCCAATGTAGCGAACTTTGCGGAGTTAATCACGGTTTTATG
CCCATCGTAGTTCAAGCGGTTAGTCAAGATGAATACTTAACTTGGGTTGG
TAAAAGATTATGCTCTTAA
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Coding sequence (CDS) from alignment at H-paniculata_contig76933:216..584-

>mRNA_H-paniculata_contig76933.15702.1 ID=mRNA_H-paniculata_contig76933.15702.1|Name=mRNA_H-paniculata_contig76933.15702.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=738bp|location=Sequence derived from alignment at H-paniculata_contig76933:216..584- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTTGATTCATATCTTATAGCTGATGAAGATTTAGTAATTCCAGAGGCTTT
AGGTACGGGTAAAGCAGGTAAAGTTTTCAGTCTACTCGAAGTAGATAATC
GTTTATTTGTCCCAACTAATACACATATACGTATGCTTATTACTTCTGCG
GATGTTCTTCATTCGTGGGCTGTACCAAGCTTAGGCGTTAAAGTAGATGC
TTGTCCTGGTCGTTTAAATCAAGTATTTATGTTTGTTAAGCGGGAAGGTG
TTTTCTATGGCCAATGTAGCGAACTTTGCGGAGTTAATCACGGTTTTATG
CCCATCGTAGTTCAAGCGGTTAGTCAAGATGAATACTTAACTTGGGTTGG
TAAAAGATTATGCTCTTAATTTGATTCATATCTTATAGCTGATGAAGATT
TAGTAATTCCAGAGGCTTTAGGTACGGGTAAAGCAGGTAAAGTTTTCAGT
CTACTCGAAGTAGATAATCGTTTATTTGTCCCAACTAATACACATATACG
TATGCTTATTACTTCTGCGGATGTTCTTCATTCGTGGGCTGTACCAAGCT
TAGGCGTTAAAGTAGATGCTTGTCCTGGTCGTTTAAATCAAGTATTTATG
TTTGTTAAGCGGGAAGGTGTTTTCTATGGCCAATGTAGCGAACTTTGCGG
AGTTAATCACGGTTTTATGCCCATCGTAGTTCAAGCGGTTAGTCAAGATG
AATACTTAACTTGGGTTGGTAAAAGATTATGCTCTTAA
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