mRNA_H-paniculata_contig475.11797.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: D8LBA4_ECTSI (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LBA4_ECTSI)

HSP 1 Score: 761 bits (1964), Expect = 1.050e-267
Identity = 420/629 (66.77%), Postives = 446/629 (70.91%), Query Frame = 2

Query:  197 SRPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPG----------KINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRA-GDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVA-------------EPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            SR FVV+VAA AGSLGIGKNGALPW LA DMAYFK+ TSTP T            KINAVIMGRKTWQSIP RFRPLAGRRN+VLSRNPAAR  L LP DVLLAGSL EAL LL+ A   +EVDKVFVIGGG VY EA            VA  LCEKVLLTSV    DGRFDDCD HFP L P+ FKLV +   QEEKGVE SFDEY+ + H     SE +A  A                       AAE DKE A+                    AS   SKRK+      +        AA                           E  E    N+EEMQYL LVEDIL+NGV RGDRTGTGTLSKFGVQMRFSLR+  FPLLTTKRVFWRGVAEELLWF++GCTNANVLK RGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADY GKGVDQLAECI+KIKTNPEDRRIVLSAWNP DL KMALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASYALLTRLVAQAC LKAG+ VHTIGDAHVYVNHVDAL+EQL+RKPRPFPTL + +  TDIDSF Y  LE+KGY P+K IKMKMAV
Sbjct:   13 SRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGXXXXXXXTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPAA-ASEVDKVFVIGGGSVYAEA------------VASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVELSFDEYESVNH-----SEAAAAMA-----------------------AAEGDKENASA-------------------ASVVGSKRKSSPDGDQVAATGVETPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXEEAEGGPGNEEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIQKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 581          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A835Z035_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z035_9STRA)

HSP 1 Score: 649 bits (1675), Expect = 6.400e-225
Identity = 348/609 (57.14%), Postives = 406/609 (66.67%), Query Frame = 2

Query:  188 AAPSRPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRA-GDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            AA  R FVVVVAA AGSLGIGK G +PW LAGDMAYFKKVTS     GK+NAV+MGRKTW+SIP RFRPL GR N+VL+RNP AR +L+LPDDV++A SL +AL+ L+      +V K+FVIGGG VY+EALG+              C++V LT+V A  DG+  +CD  FP L P  FKLVSR   Q E G++Y F+ Y+R         + SA PA G+                   + A                    NG         +RS    D S A I +E                             NDEE QYL LV+DI++NG LRGDRTGTGTLSKFG QMRFSLR   FPLLTTKRVFWRGVAEELLWF++GCT+A  L+ + IHIWDGNGSR FLD  GL  REEGDLGPVYGFQWRHFGA+Y D   DYKG+GVDQLA+CI KIK +P+DRRI++SAWNP DLE MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASY+LLT +VAQ C LK G+ +HTIGDAHVYVNHVDAL+EQ++R PRPFP L +  + TDIDSF   D  ++GY P   IKM+MAV
Sbjct:    2 AAALRDFVVVVAATAGSLGIGKGGGIPWKLAGDMAYFKKVTSACDIAGKVNAVVMGRKTWESIPQRFRPLPGRCNVVLTRNPRARQELVLPDDVIIARSLEDALSCLSTQDMRDKVHKIFVIGGGQVYKEALGMEQ------------CKQVHLTTVEAPADGKQFECDTFFPPLDPASFKLVSRGDKQSENGIDYRFNVYER--------QDSSAAPASGS----------------FSSVGAT-------------------NG---------SRSSSAKDTSGAAIAEE----------------------------PNDEERQYLDLVQDIIDNGALRGDRTGTGTLSKFGAQMRFSLRGGRFPLLTTKRVFWRGVAEELLWFVAGCTDAGALQAKNIHIWDGNGSRAFLDSRGLEHREEGDLGPVYGFQWRHFGAKYVDFRTDYKGQGVDQLADCIHKIKNSPDDRRIIMSAWNPCDLELMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMVAQVCGLKPGDFIHTIGDAHVYVNHVDALREQIKRVPRPFPKLRLHPARTDIDSFEMKDFTLEGYNPMNAIKMQMAV 518          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A4D9D1L8_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D1L8_9STRA)

HSP 1 Score: 607 bits (1566), Expect = 2.470e-208
Identity = 334/607 (55.02%), Postives = 382/607 (62.93%), Query Frame = 2

Query:  200 RPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGR---FDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            R F +VVAA A + GIGK G LPW+L  DM +FK++T+  + P KINAVIMGR+TWQSIP +FRPL  R N+VLSRNP  R QL LP  V +A SL  ALALLA     + V+KVFVIGG  VYREA            V    CE +  TS+    G    F DCD HFPV+    + LVS +PV  +  + + F  Y+R+   D G S  S    VG G                                                                        P+  S A G                 N EE QYLRLV++I+E+G  RGDRT TGTLSKFG Q+RFSLRD  FPLLTTKRVFWRGVAEELLWFISG T+AN LK + I IWDGN SRE+LD LGL+ REEGDLGPVYGFQWRHFGAEY+DMHADY GKG+DQLA  I  IK NPEDRRI++SAWNP DL KMALPPCHMFCQFYVA+GELSCQMYQRSADMGLGVPFNIASYALLTRLVAQ C LKAGE +HTIGDAHVY+NHVDALKEQL R PR FP L +  +   IDSF++ D  I+GYLPH+ +KMKMAV
Sbjct:   26 RGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREA------------VISEACEAIEFTSIEEKRGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERL---DRGPSADSP---VGGG------------------------------------------------------------------------PSKVSGAAG-----------------NQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKHIDSFTFEDFTIEGYLPHESLKMKMAV 525          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A7S2MDM9_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MDM9_9STRA)

HSP 1 Score: 598 bits (1541), Expect = 2.250e-204
Identity = 325/605 (53.72%), Postives = 396/605 (65.45%), Query Frame = 2

Query:  200 RPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDDCDVHFPVLAPERFKLVSRS-PVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            R F V+VAA A + GIG+ G LPW +  DMA+FK+ T T    G +NAVIMGRKT+QSIP RFRPL GR N+V+SRNP  R +L LPDDVL+AGSL++ALA++        V +VFVIGGG +Y EA            V   LC +V++T+V +    F+DCDV FP L  + + L S S PV   + +  +F+ ++RI                                        ++D+                       + +      +A ++S  +      P++AS  V              E ++  EEMQYL L+ DI+++GV RGDRTGTGTLSKFGVQMRFSLR+N+ PLLTTKRVFWRGVAEELLWF+SG TNA  L+++ I IWDGNGSREFLD  GL  REEGDLGPVYGFQWRHFGA+YTDMHADY G+GVDQLA+ I  IK NP DRRI++SAWNP  L++MALPPCHMFCQFYVA+G LSCQMYQRSADMGLGVPFNIASYALLTRLVA+ CDL AGE +HTIGDAHVY+NHVDAL+ QL R PRPFP L++     DIDSFSY DL ++GY PH  IKMKMAV
Sbjct:    2 RTFDVIVAATASTFGIGRRGRLPWKIGVDMAFFKRATLTCDMDGMMNAVIMGRKTYQSIPARFRPLQGRVNVVISRNPNLRQELELPDDVLVAGSLSDALAMIGEGTLNDRVSEVFVIGGGSIYEEA------------VKSQLCSRVVMTTVHSTG--FEDCDVSFPSLPSDEYILTSASAPVTTSEELVVNFNVFERI------------------------------------KKKTKEDEXXXXXXXXXXXXXXXXXXXXXXXELNALLKTSQAPMTSQLLP-----PSSASTLV--------------ESMAAHEEMQYLNLIRDIIDHGVQRGDRTGTGTLSKFGVQMRFSLRNNVLPLLTTKRVFWRGVAEELLWFVSGSTNAKDLQDKNIRIWDGNGSREFLDSRGLGHREEGDLGPVYGFQWRHFGAQYTDMHADYSGQGVDQLAKAIDAIKNNPTDRRILVSAWNPAALDEMALPPCHMFCQFYVADGALSCQMYQRSADMGLGVPFNIASYALLTRLVARVCDLDAGEFIHTIGDAHVYLNHVDALRTQLTRIPRPFPRLVINEEKRDIDSFSYEDLTVEGYSPHGTIKMKMAV 537          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A7S2WWT1_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WWT1_9STRA)

HSP 1 Score: 591 bits (1523), Expect = 2.200e-201
Identity = 337/606 (55.61%), Postives = 406/606 (67.00%), Query Frame = 2

Query:  206 FVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSA-GPAVG-AGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDE--NGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            F ++VAA A SLGIG+ G LPW L  DMA+FK+ T +   PG+ NAVIMGRKT+QSIP RFRPL GR N+V+SRNP  R +L LP +VL+A SL +AL  L  +     V +V+VIGGG +Y EA            VA   C  +L+T V + +  F+DCD HFP +   RF L+S S    +KGV  SF+ ++R+     G     A GPA   AG      XXXXXXXXXXX          AT    A Q T  E  +G+L +                              + V    G+  V       ++   EMQYL L++DI++NGV+RGDRTGTGT+SKFG QMRFSLR + FPLLTTK+VFWRGVAEELLWF+SG TNA +L+++ I IWDGN SREFLDG GL  REEGDLGPVYGFQWRHFGA Y DMHA+Y GKGVDQL + I  IK+ P DRRI++SAWNP DL+ MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASY+LLTR++AQ C LKAG+ +HTIGDAHVY+NHVDAL+EQL R PRPFP L +    ++IDSF +SD  ++GY PH  IKMKMAV
Sbjct:    5 FDIIVAATASSLGIGRQGKLPWRLGVDMAFFKRATLSAGLPGQSNAVIMGRKTYQSIPARFRPLNGRVNVVISRNPEIRAELDLPAEVLVASSLDDALDQLGSSKVGQAVSQVYVIGGGSIYDEA------------VAHEACRHLLVTMVHSEE--FEDCDTHFPAIDTARFSLLSSSAPFTDKGVVCSFNVFERV-----GSLRCFANGPASPVAGXXXXXXXXXXXXXXXXXXXXXXXXXXXATAE--APQLTPSEILDGLLEKP-----------------------------VEVPRGPGVNLVGS-----MAPHPEMQYLALIQDIIDNGVVRGDRTGTGTVSKFGAQMRFSLRGDEFPLLTTKKVFWRGVAEELLWFVSGSTNAKLLQDKKIKIWDGNASREFLDGRGLHHREEGDLGPVYGFQWRHFGARYEDMHANYDGKGVDQLMQAINTIKSRPNDRRIIVSAWNPADLDLMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTRMMAQVCGLKAGDFIHTIGDAHVYLNHVDALREQLARAPRPFPKLRINPDKSEIDSFDFSDFTVEGYKPHGTIKMKMAV 555          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A1Y1HPI3_KLENI (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1HPI3_KLENI)

HSP 1 Score: 580 bits (1496), Expect = 6.160e-197
Identity = 318/608 (52.30%), Postives = 397/608 (65.30%), Query Frame = 2

Query:  194 PSRPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAAR-GQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVG-AGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            P R F +VVAA    LGIG+NG+LPW L GDM +FK+VT+T ++P K NAV+MGRKTW SIP +FRPL GR N+VL+R  +        P+ VL+  SL  A++ LA A H  +V+ VFVIGGG +YREA+  P            LC+ V LT V   D   + CD   P + P +F+  S SP + E  + YSF  Y R    +  +   + G AV  +G+V E                  +    A L      A R E G  A+ D            +   ++ +     A S + GA  G G VA   +      EE+QYL+L+++I+  G  +GDRTGTGT+SKFG  MRF+LR + FPLLTTKRVFWRGV EELLWF++G TNA +L++RG+HIWDGNGSR +LD +GLT REE DLGPVYGFQWRHFGA+YTDMHADY G+GVDQLA+ I +IKT PEDRR++LSAWNP DL+ MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQ C L+ G+ +H +GDAHVY NHV+ L+EQLR  PRPFP L V  +  DIDSF+  D E+ GY PHK I MKMAV
Sbjct:    9 PKRTFQIVVAATQ-DLGIGRNGSLPWKLPGDMRFFKQVTTTTSSPDKRNAVVMGRKTWDSIPPKFRPLPGRLNVVLTRAASKTDSDSAYPEGVLVCASLDSAMSHLASADHADKVETVFVIGGGQIYREAMASP------------LCDAVHLTEVNTRDTPLE-CDTFMPPVDPSQFRAWSASPPKVENDLRYSFVTYVRTHAPEEPQGVETNGAAVAPSGVVVE-------------STGRVESAGGAELGAGESAAERPERG--AEGDGXXXXXXXXXXSAQLGVLGK-----AKSGSDGAT-GPGAVARLPKSIQEKHEELQYLKLIDEIIREGAHKGDRTGTGTISKFGCSMRFNLRKS-FPLLTTKRVFWRGVVEELLWFVAGSTNARLLQDRGVHIWDGNGSRAYLDSIGLTHREEHDLGPVYGFQWRHFGAKYTDMHADYTGQGVDQLADLIHRIKTKPEDRRLILSAWNPADLQLMALPPCHMFCQFYVADGELSCQMYQRSCDMGLGVPFNIASYSLLTLMIAQVCGLRPGDFIHVLGDAHVYTNHVEPLQEQLRNTPRPFPMLKVNPAVNDIDSFTADDFELIGYEPHKKIAMKMAV 580          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: F0YJX9_AURAN (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YJX9_AURAN)

HSP 1 Score: 573 bits (1478), Expect = 2.820e-195
Identity = 321/599 (53.59%), Postives = 370/599 (61.77%), Query Frame = 2

Query:  215 VVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            +VAA AGS GIGK+G LPW LAGDM YFKKVTS  A  GK NA +MGRKTW SIP +FRPL GR+N+VLSRNP AR +L LP DV +  SL  A+  LAG    A++ +VFVIGGG VY  AL            A   C KVLLTS+    G F DCD  FP  A   F   +      E GV Y F+   R              PA                             +K  L        R+ NG  A   A+                                         E+    N EE QYL L+ +IL+ GV RGDRTGTGT+S FG +M FSLR++ FPLLTTKRVFWRGVAEEL+WF+SG TN  +L ++GIHIWDGNGSREFLD  GL  REE DLGPVYGFQWRHFGA+Y DMH DY G+GVDQLA C++ IK +P  RRIVLSAWNP DL++MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTR+VAQA  LK G+ VH IGDAHVY NHVDALK QL+R+PRPFPTL +  + T ID F ++D  ++GY P+  I MKMAV
Sbjct:   20 IVAAVAGSYGIGKDGTLPWKLAGDMKYFKKVTSA-APEGKTNACVMGRKTWLSIPPKFRPLGGRKNVVLSRNPNAREELGLPPDVFVCPSLDAAVDALAGE---ADLGEVFVIGGGQVYASAL------------ADARCTKVLLTSIDDAAGAFADCDAVFPDPAKAGFASAASGEALVENGVAYRFETLTR--------------PA-----------------------------KKPPL--------RESNGPPAPPPAN-----------------------------------------EKPAAPNHEEKQYLDLIAEILDRGVRRGDRTGTGTISIFGARMSFSLRNDRFPLLTTKRVFWRGVAEELVWFVSGSTNGKLLADKGIHIWDGNGSREFLDRRGLGHREEMDLGPVYGFQWRHFGAKYVDMHKDYAGEGVDQLAACVETIKQDPNSRRIVLSAWNPADLDEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQATGLKPGDFVHVIGDAHVYSNHVDALKVQLQREPRPFPTLTINPAKTGIDDFEFADFTVEGYKPYPKIDMKMAV 510          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A7R9UC25_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=2 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UC25_9STRA)

HSP 1 Score: 574 bits (1480), Expect = 5.110e-195
Identity = 321/608 (52.80%), Postives = 378/608 (62.17%), Query Frame = 2

Query:  200 RPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAA-RGQLLLPDDVLLAGSLAEALALLA-GAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDD--CDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            R FVVVVAA A SLGIGK+G LPW + GDM++FKK+TS    PG  NAVIMGRKTW+SIP +FRPL+GR NIVLSR+P + RGQL  P+ V +A SL EALA++A G  H  +V+ VFVIGGG VY +AL                C++++LT V   +G  +D  CD   P +    +  V+ S  Q ++   YSF  Y+R               A GA                    AA     +A    +AQ  T  ++                                                           E QYL LV DI+E+GV RGDRTGTGTLSKFGVQMRFSLRD  FPLLTTKRVFWRG+AEELLWFI GCTNANVL  + IHIWDGNGSREFLD  GLT+RE GDLGPVYGFQWRHFGA+Y DMHADY G+GVDQL + I  +K NPE RR +L+AWNP DL KMALPPCHM  QFYVA+GELSC +YQRS DMGLGVPFNIASYALLTR++AQ   L+ G+ VH IGDAHVY+NHV+ LKEQLRR+P PFPTL V S  TDIDSF +SD  +  Y  H+ +KM MAV
Sbjct:   46 RHFVVVVAATASSLGIGKDGKLPWRIRGDMSFFKKLTSETRIPGHRNAVIMGRKTWESIPSKFRPLSGRLNIVLSRHPESLRGQL--PEQVAVASSLEEALAMVAPGGIHCDQVEDVFVIGGGSVYADAL------------RSSYCKRIVLTEVTPLEGTEEDLGCDTFLPRIDGSAYMRVAESGDQVDENFRYSFVTYERR--------------ATGA--------------------AATDGPPQAQAQPQAQARTNPDHA----------------------------------------------------------EWQYLDLVRDIIESGVERGDRTGTGTLSKFGVQMRFSLRDGSFPLLTTKRVFWRGLAEELLWFIQGCTNANVLAEKNIHIWDGNGSREFLDKRGLTDREVGDLGPVYGFQWRHFGAQYRDMHADYAGEGVDQLRQIISDLKANPESRRHILTAWNPSDLPKMALPPCHMMAQFYVADGELSCLLYQRSGDMGLGVPFNIASYALLTRIIAQVTGLQPGDFVHCIGDAHVYLNHVEPLKEQLRREPLPFPTLEVNSKKTDIDSFEFSDFVLHNYRHHRGVKMDMAV 547          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: A0A8J2SV63_9STRA (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SV63_9STRA)

HSP 1 Score: 568 bits (1463), Expect = 4.650e-193
Identity = 315/601 (52.41%), Postives = 375/601 (62.40%), Query Frame = 2

Query:  209 VVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPAARGQLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFDDCDVHFPVLAPERFKLVSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            V  + AA   +GIG++GALPW L GDMAYFK+VT T A   + NAV+MGRKTW SIP +FRPL GR N+VLSRNP AR QL LP+DV    SL  AL  +     VA   KVFVIGGG +Y+ A            V    C +VLLT V    G    CD  FP L    F  V+ SP ++E  + YSF                                                                     L +E +ST    +  DV+++   +    P            + PV         N EE QYL L+ +IL++GV+RGDRTGTGT S FG +M+FSLRD++FPLLTTKRVFWRGVAEELLWF+SG TN  +L+ + IHIWDGNGSREFLD  GL  REE DLGPVYGFQWRHFGA Y DMHADY GKGVDQLAECI++IK +P  RRIVLSAWNP DL +MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTR+VAQ+ +LKAG+ VH IGDAHVY+NH+DAL+EQL+R PRPFP+L + ++  DID+F Y D  + GY P+  I+M+MAV
Sbjct:   13 VACIVAATAQMGIGRDGALPWRLKGDMAYFKRVT-TEAPASQQNAVVMGRKTWLSIPKKFRPLPGRANVVLSRNPDAREQLDLPEDVACYASLDAALTQMKAREDVA---KVFVIGGGELYKAA------------VNDARCTEVLLTRVEDPKGVLPQCDAFFPDLTGTGFTTVAMSPPEKEGDLAYSFQ-------------------------------------------------------------------TLTRECSST----KLGDVTNSLKEKLVEKPPP----------VPPV---------NKEEQQYLDLIREILDSGVVRGDRTGTGTKSIFGARMKFSLRDDVFPLLTTKRVFWRGVAEELLWFVSGATNGKLLQEKNIHIWDGNGSREFLDKRGLPHREEMDLGPVYGFQWRHFGASYEDMHADYAGKGVDQLAECIRQIKGDPNSRRIVLSAWNPADLPEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQSTNLKAGDFVHVIGDAHVYLNHIDALEEQLKRAPRPFPSLTINAAKKDIDAFEYDDFTVDGYKPYPKIQMEMAV 507          

BLAST of mRNA_H-paniculata_contig475.11797.1 vs. uniprot
Match: C1MMA6_MICPC (Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Micromonas pusilla (strain CCMP1545) TaxID=564608 RepID=C1MMA6_MICPC)

HSP 1 Score: 563 bits (1450), Expect = 7.680e-191
Identity = 317/622 (50.96%), Postives = 375/622 (60.29%), Query Frame = 2

Query:  197 SRPFVVVVAAAAGSLGIGKNGALPWHLAGDMAYFKKVTSTPATPGKINAVIMGRKTWQSIPGRFRPLAGRRNIVLSRNPA------------ARG--QLLLPDDVLLAGSLAEALALLAGAPHVAEVDKVFVIGGGMVYREALGLPSAEGLTSRVAQGLCEKVLLTSVRAGDGRFD--DCDVHFPVLAPERFKL-VSRSPVQEEKGVEYSFDEYDRIFHADTGKSEHSAGPAVGAGIVSEGGXXXXXXXXXXXDIAAEQDKEKATLSCRAQQATRDENGVLAQEDASTARSKRKADVSSACIVQESSAPTAASLAVGAKAGIGPVAEPEEERVSNDEEMQYLRLVEDILENGVLRGDRTGTGTLSKFGVQMRFSLRDNLFPLLTTKRVFWRGVAEELLWFISGCTNANVLKNRGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYKGKGVDQLAECIKKIKTNPEDRRIVLSAWNPKDLEKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACDLKAGELVHTIGDAHVYVNHVDALKEQLRRKPRPFPTLLVKSSNTDIDSFSYSDLEIKGYLPHKPIKMKMAV 2011
            +RPF VVVAA  G +GIG +GALPW L  DMAYFK VT+T A PGK NAVIMGRKTW+SIP +FRPL GR N+VLS++ A            A G    +LP  VLL  SL  ALA L    H   V+K+FV+GG  VY EA+  P+            C+ V +T V       D   CD H P +  E+FKL  S  PV E++G +  F  Y     A TGK       ++  G V+                                      NGV                                                        EE+QYL L+ DI+E+G ++GDRTGTGT+SKFG  MRF LR + FPLLTTKRVFWRGVAEELLWF+ G TNA  L+++ IHIWDGNGSRE+LD +GL+ REE DLGPVYGFQWRHFGAEYTDMHA+Y GKGVDQLAE I KIK NP DRRI+L+AWNP  L++MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT ++AQ C LK G+ VH +GD HVY NHV+ LK+QL  +PRPFPTL +  + TDIDSF +SD  I+GY PH  I+MKMAV
Sbjct:    9 TRPFQVVVAATKGDMGIGLDGALPWRLPKDMAYFKSVTATVAEPGKRNAVIMGRKTWESIPAKFRPLPGRLNVVLSKSGALVESTGSENAAPANGTPSNVLPPSVLLQPSLEAALATLGAGEHAENVEKIFVVGGAQVYAEAMASPA------------CQAVHITEVTPPKDTPDAFKCDTHMPPVDMEKFKLYASAPPVNEKEGAKIQFLSYVAK-DAATGKFRIPGSVSLPPGAVA--------------------------------------NGV------------------------------------------------------RHEELQYLDLIRDIIESGNVKGDRTGTGTISKFGTSMRFDLRRS-FPLLTTKRVFWRGVAEELLWFVGGRTNAKELQDKDIHIWDGNGSREYLDSVGLSHREEQDLGPVYGFQWRHFGAEYTDMHANYDGKGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCGLKPGDFVHVMGDTHVYSNHVEPLKKQLLCEPRPFPTLKINPAKTDIDSFEFSDFTIEGYDPHPKIEMKMAV 524