mRNA_H-paniculata_contig4423.11185.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig4423.11185.1
Unique NamemRNA_H-paniculata_contig4423.11185.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: D7FHY4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHY4_ECTSI)

HSP 1 Score: 276 bits (706), Expect = 4.970e-82
Identity = 134/190 (70.53%), Postives = 147/190 (77.37%), Query Frame = 2 
Query:   53 SPDDIAQRVREMEEEILRKLLAEEDAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLRCEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQC---------------------EDTWDPSDEQAARRFVAAVGAALGGARRAYETRVAPPTDSECSFSLETRRCEPQDLCRLRARWGDLTLSSACRL 559
            SP+D+AQRVR+ME E++RKLLAEEDAKSLLDIWVE  +E+VLT+TVPPTDA+CDFNWSRLRCEPKC CG RLRFGDYTPGRACRLLHPWE +P+                         WDPSDE A RR VAA   ALGG R+AYE RVAPP+DSECSFS ETRRCEPQ LCRLR RWGDLT SSACRL
Sbjct:  462 SPEDMAQRVRDMEAELVRKLLAEEDAKSLLDIWVERTREYVLTQTVPPTDAECDFNWSRLRCEPKCTCGARLRFGDYTPGRACRLLHPWERNPELCGSGVDGDDXXXXXXXXXXXXXXXWDPSDEPAVRRVVAAAAGALGGLRQAYEERVAPPSDSECSFSFETRRCEPQPLCRLRFRWGDLTPSSACRL 651
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: A0A6H5JIE4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JIE4_9PHAE)

HSP 1 Score: 275 bits (703), Expect = 3.260e-80
Identity = 133/191 (69.63%), Postives = 147/191 (76.96%), Query Frame = 2 
Query:   53 SPDDIAQRVREMEEEILRKLLAEEDAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLRCEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQC----------------------EDTWDPSDEQAARRFVAAVGAALGGARRAYETRVAPPTDSECSFSLETRRCEPQDLCRLRARWGDLTLSSACRL 559
            SP+++AQRVR+ME E++RKLLAEEDAKSLLDIWVE  +E+VLT+TVPPTDA+CDFNWSRLRCEPKC CG RLRFGDYTPGRACRLLHPWE +P+                          WDPSDE A RR VAAV   LGG R+AYE RVAPP+DSECSFS ETRRCEPQ LCRLR RWGDLT SSACRL
Sbjct:  626 SPEEMAQRVRDMEAELVRKLLAEEDAKSLLDIWVERTREYVLTQTVPPTDAECDFNWSRLRCEPKCTCGARLRFGDYTPGRACRLLHPWERNPELCGSGVNGDDGXXXXXXXXXXXXXXAWDPSDEPAVRRVVAAVAGVLGGLRQAYEARVAPPSDSECSFSFETRRCEPQPLCRLRFRWGDLTPSSACRL 816
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: A0A7S2UXV6_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UXV6_9STRA)

HSP 1 Score: 100 bits (248), Expect = 6.600e-21
Identity = 58/159 (36.48%), Postives = 80/159 (50.31%), Query Frame = 2 
Query:  149 WVENAKEFVLT----KTVPPTDAQCDFNWSRLRCEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQCEDTWDPS---DEQAARRFVAAVGAALGGARRAYETRVAPPTDSECSF----SLETRR--CEPQDLCRLRARWGDLTLSSACRLTKNKDENST 586
            W E   + VLT    K VP TD +C ++W   RC+P+C C ++ +FGDY+PGRACR L   E  P C    DPS   D     +F   V           +T + P TD +C F    S ++RR  C P   C  + ++GDLT+  ACR    ++  ST
Sbjct:   31 WAEKISDKVLTFVNEKVVPDTDPECTWHWGHWRCDPQCECKLKYKFGDYSPGRACRSLTFGELDPNC----DPSAGDDISLLEKFGRVVVVTWRRVALFSKTYLLPRTDDQCQFAWKESWKSRRPTCSPHASCSFQPKFGDLTVGRACRYKYKEESKST 185
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: A0A6H5JPF6_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JPF6_9PHAE)

HSP 1 Score: 94.4 bits (233), Expect = 8.640e-18
Identity = 54/162 (33.33%), Postives = 82/162 (50.62%), Query Frame = 2 
Query:  125 DAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLRCEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQCEDTWDPSDEQAARRFVAAVGAALGGARRAYETRVAPPTDSECSFSL----ETRR--CEPQDLCRLRARWGDLTLSSACRLTKNKDENSTAI 592
            D ++LL+       ++V T+  P TD +CD++W R RC+P+C C  + RFGDY PGR+CRL    E      D    +D     +  A VG       R     V P TD +C FSL    E ++  C P+  C    ++GD+    +CRL   +D++ T++
Sbjct:   91 DDEALLERIGRLVVQWVNTRLTPRTDEECDWSWGRWRCDPQCSCQYKYRFGDYAPGRSCRLRPEGERGLATCDPERGNDVSLLEKLAAFVGKVGRSTWRFTAEHVVPQTDKDCRFSLAHSLEEKKLVCTPRRHCAFHYKFGDVHPGRSCRL---RDKSGTSV 249
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: A0A7R9ZJ95_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZJ95_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 1.550e-11
Identity = 36/85 (42.35%), Postives = 51/85 (60.00%), Query Frame = 2 
Query:  116 AEEDAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLRCEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQCEDTWDPSDEQA 370
            AE+D ++LLD++ E AKEF+  K +PPTD  C ++W   RCEP C C    + GDY  GR+CR     +    C++  DP+  QA
Sbjct:   96 AEQDDRTLLDVFGEAAKEFLTQKLIPPTDTDCRWDWRFARCEPFCACAFAPQRGDYHLGRSCRR----KTKVACDEPQDPTKVQA 176
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BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Match: A0A7S0CLR0_9STRA (Hypothetical protein n=2 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0CLR0_9STRA)

HSP 1 Score: 60.5 bits (145), Expect = 2.790e-6
Identity = 28/71 (39.44%), Postives = 41/71 (57.75%), Query Frame = 2 
Query:  113 LAEEDAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLRCEPKCVCG-----MRLRFGDYTPGRACRLL 310
            L E+D ++ LD + E +K  +    +P TDA+CDF+W  LRCEP  +CG         FGD+  GR+C + 
Sbjct:  141 LDEDDERTYLDTFGEVSKIIMTHHVIPETDAECDFDWRSLRCEPHDICGGGGCRFEYEFGDFHLGRSCWIF 211
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig4423.11185.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FHY4_ECTSI4.970e-8270.53Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JIE4_9PHAE3.260e-8069.63Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S2UXV6_9STRA6.600e-2136.48Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A6H5JPF6_9PHAE8.640e-1833.33Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7R9ZJ95_9STRA1.550e-1142.35Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
A0A7S0CLR0_9STRA2.790e-639.44Hypothetical protein n=2 Tax=Proboscia inermis Tax... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig4423contigH-paniculata_contig4423:3223..6228 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score260.8
Seed ortholog evalue4.8e-67
Seed eggNOG ortholog2880.D7FHY4
Hectar predicted targeting categoryother localisation
EggNOG OGs2E73F@1,2SDR3@2759
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-12_006360.1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons3
Model size1415
Cds size537
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig4423.11185.1prot_H-paniculata_contig4423.11185.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig4423 3308..5435 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930334.2410614-UTR-H-paniculata_contig4423:3222..33071622930334.2410614-UTR-H-paniculata_contig4423:3222..3307Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig4423 3223..3307 +
1691481246.3041537-UTR-H-paniculata_contig4423:3222..33071691481246.3041537-UTR-H-paniculata_contig4423:3222..3307Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig4423 3223..3307 +
1622930334.3630557-UTR-H-paniculata_contig4423:5435..62281622930334.3630557-UTR-H-paniculata_contig4423:5435..6228Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig4423 5436..6228 +
1691481246.3500724-UTR-H-paniculata_contig4423:5435..62281691481246.3500724-UTR-H-paniculata_contig4423:5435..6228Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig4423 5436..6228 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930334.2703445-CDS-H-paniculata_contig4423:3307..33691622930334.2703445-CDS-H-paniculata_contig4423:3307..3369Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 3308..3369 +
1691481246.3148913-CDS-H-paniculata_contig4423:3307..33691691481246.3148913-CDS-H-paniculata_contig4423:3307..3369Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 3308..3369 +
1622930334.3101852-CDS-H-paniculata_contig4423:4468..48441622930334.3101852-CDS-H-paniculata_contig4423:4468..4844Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 4469..4844 +
1691481246.3256302-CDS-H-paniculata_contig4423:4468..48441691481246.3256302-CDS-H-paniculata_contig4423:4468..4844Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 4469..4844 +
1622930334.3464127-CDS-H-paniculata_contig4423:5336..54351622930334.3464127-CDS-H-paniculata_contig4423:5336..5435Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 5337..5435 +
1691481246.3369486-CDS-H-paniculata_contig4423:5336..54351691481246.3369486-CDS-H-paniculata_contig4423:5336..5435Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig4423 5337..5435 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig4423.11185.1

>prot_H-paniculata_contig4423.11185.1 ID=prot_H-paniculata_contig4423.11185.1|Name=mRNA_H-paniculata_contig4423.11185.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=179bp
MEEEILRKLLAEEDAKSLLDIWVENAKEFVLTKTVPPTDAQCDFNWSRLR
CEPKCVCGMRLRFGDYTPGRACRLLHPWEHSPQCEDTWDPSDEQAARRFV
AAVGAALGGARRAYETRVAPPTDSECSFSLETRRCEPQDLCRLRARWGDL
TLSSACRLTKNKDENSTAIPNKMRARPL*
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mRNA from alignment at H-paniculata_contig4423:3223..6228+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig4423.11185.1 ID=mRNA_H-paniculata_contig4423.11185.1|Name=mRNA_H-paniculata_contig4423.11185.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=3006bp|location=Sequence derived from alignment at H-paniculata_contig4423:3223..6228+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CGTTCGTGGAACCAGTCGTGTTGGGGCGGAGGGAGGCTCAAAGAGTGCGT
CGTCGCCTGACGATATCGCACAGCGCGTGAGGGAAATGGAAGAAGAAATA
TTGCGCAAGCTTCTGGCAGAGGAGGATGCGAAGTCTCTGCTCGATATGTG
AGTGTTGTTCTCTCGGCGGAAAGCAGTCAGACTTGCGTTTGGGTGGCCTT
GGCCAGGCTTTTCAGGTTGCCGAGATTGTTCATAATATTTTACACGTGAG
CAGAATCGGAATCGGTGTGCGGTATTGCTTCGTCAGATGTTTTTATTTTG
CTACAACCGGTTCGCCGTTGGCTCTCTCCTCTGATCCAAAGCGGGTTGGG
TTGGCGGGTTGGTTCTGTCGGACTGTGTCGGTCATGGGAAAACGCTTGCT
TGTTAGTCAAACACTCGAAATTCTTGAGGATAACGACTCTTCACTTCACG
GCCGAGAGATCGTGTCCATTTATAGATCTGGACGAGATTTTGTTTCATGA
ACAAAATACAGTAGTGAAGGGAAAAAAAAAAAGCGAGTTCGAACAATAAC
AAGGCTGTTCACGTGATTGGACATTACACGTGTTTTGTTCATACGTCCAT
GGTTTCAGCCGGTTGGTGGATGGCCCTGTGAGAATTTTCATTCTGCTGTC
CGGTTCGCAGATGGTTGTGTGAGAGTATTTCCCTGTTCGCGGTATGTGAG
ATTCTGATGGCCATGTGAGATATTGACAGGTCTTGTGATAACTCTTGAAA
ATTATCAGAATTTTTCACGTGACCGGCAACACGAAAGACTTTTGACATGT
GACAAGAATTGGAAACGGTTCATCGTAGAGTTCTGTAAATGTTTTTTCAT
TTCTACGACCGGTTCGCGGTCGGTGTGTCTGATTCAAGCAGGTCGGCGGC
CGGTTTGGTCAGGCATTCATGTGTACAACCGGTTCGCGGTTGGTTCTGTC
TAAACTTCATCCGGTGGGCGGTTGACTCGATTTCAATATTGCGGTGTGAG
TTTTAGTTGAGAACCATCGTGAATGTTTCTGTATTATGTGTTAAAAACAT
ATTTTGGCTTGATTCCATTTACAAGGCACTGTTGCACTATTCGAATTCGT
AAGCTAGAAATCAACTCATTGACACACACACACACATACACACACAATCC
CGTCCTACGCCGTACTTCCCCCCTCCTCCTGGGCCTGTAACGCCCCCCCC
ACCCCATTGCGTCATCACCATCACCACCACCAACCCACCCGCTCAGCTGG
GTCGAGAATGCCAAAGAATTTGTGCTCACGAAAACAGTACCACCTACGGA
CGCCCAATGCGACTTCAACTGGAGCCGCCTACGCTGCGAACCCAAGTGTG
TGTGCGGTATGCGCCTACGCTTCGGAGATTACACGCCTGGTAGGGCCTGC
CGCTTATTGCATCCGTGGGAGCACAGCCCTCAATGCGAGGACACCTGGGA
CCCCTCGGACGAACAGGCGGCGAGGCGTTTCGTGGCGGCGGTGGGTGCGG
CGCTCGGCGGCGCTCGGCGCGCTTACGAGACGCGAGTGGCGCCCCCGACA
GACTCAGAGTGTAGCTTCAGTTTGGAAACCCGCCGCTGCGAGCCACAAGA
TCTGTGTCGGCTGCGGGCACGGGTGAGTTATCTGCTGTAGCAGAATTTGT
TTATTAGCATGATTTGCGAGGCCGCGGCATGCCATACGCCGGTACTTTGT
TCTTTTGCGGCACCTAGTCAGTCAGATAATCGGCTGTGAAAATCGTATTG
TCGTATTTCCGTATGACATCTGATGCTGCCGGCAATCCAGGTAGGCGGTC
GTAGTCTTCGCAATCAAATTGACCTCGCTAAATCTGGATATCATCTGGGA
GCTGAAGGGTAGCATGTTTGTCGTGTATAGGTCAAATTTTGGCCTCGCCT
GGTCGTACAACTGAAAGCACGAGGAGCTGGAAATCTCACGTACTGTCAGC
AACGTTTTTGGACCGCTGCGGTATAATTTGTATGCTGGGATGACTGCAGT
TATGTGCAGTAAGGCACGCTGAGCTGATGATGATGTGCTGCACTGCTGTA
CTCACGTGTCCCGCGTATTCCCACCTTCTCCCTGCCTTTTTCTCTTTTTC
TTTTCTTGATACAGTGGGGAGACTTGACGTTGAGCAGCGCATGCAGGCTC
ACCAAGAACAAGGATGAAAATTCTACAGCAATACCAAACAAGATGAGAGC
CAGGCCTTTGTAATATTTAGAGAAGTAGTGCTATCATCGCTGTCGGTTTC
GGTTTCTTTGATCGATTTTCGAGAGAGGTCCGATAAGAATGGCGGGTGAT
GTTTCCAAAGGGAGAGCGTGCATGGTCCTCGTGACGGTGGTACCATCAAA
ACAAACAGTGTAGGGAGAGTCGTGGTGGGCATGGTGATCGACGGAGAGCT
TCACATGTACGCCGTGTAGTAGGTCGGTCCCTTGGAAAGCCCCTCGCCTC
TTTGTATCCCATCCTTTGCGCACAAGGAAACAAGTCGTTTTCTTGGCACT
GTTGTACCGTACCGCTATGTGTATATATGGTACTGGTTGTTGTAGTCGTG
GGTCATATCCTCGTTCTCGGCACGATCTCTGTCCATTTACAATGCTTCTG
TGTGTTTGTGCTGGTGCGCTCTGTCTAAAGTGTGGTGTGAAAGCGTGTGG
GGGGCAGGAGTTGCTGGGGTTTCTTTATAAGCATGCGCTGTCATTCCTAC
TCGGGTTCCACTCTTGCTGCAAACACTGAAGGGCTCTGAGCAACACCGGG
CGTTGTTGACTTTTTGTATAGGCCGCACATGAAAACGGGAGCATGTGCGA
GTTGTTCCCACTTTTGTTTTTCACGAGGAAATAACATGTGTGTGAAAAAA
AGGTGAAATTGGAAGAGAAAGGGAATTAATTTTGTTAGAAACGAGGAGCA
ATACATTTCATTTGATTCTCTAGCCAAGTGTAACATTATAACAAAAAAAC
AGAAACAACATTTTGCACAGTGAAAAGAATAATAATAAAAAAAGGGAGAT
GGGTGG
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Coding sequence (CDS) from alignment at H-paniculata_contig4423:3223..6228+

>mRNA_H-paniculata_contig4423.11185.1 ID=mRNA_H-paniculata_contig4423.11185.1|Name=mRNA_H-paniculata_contig4423.11185.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1074bp|location=Sequence derived from alignment at H-paniculata_contig4423:3223..6228+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGAAGAAGAAATATTGCGCAAGCTTCTGGCAGAGGAGGATGCGAAGTC
TCTGCTCGATATATGGAAGAAGAAATATTGCGCAAGCTTCTGGCAGAGGA
GGATGCGAAGTCTCTGCTCGATATCTGGGTCGAGAATGCCAAAGAATTTG
TGCTCACGAAAACAGTACCACCTACGGACGCCCAATGCGACTTCAACTGG
AGCCGCCTACGCTGCGAACCCAAGTGTGTGTGCGGTATGCGCCTACGCTT
CGGAGATTACACGCCTGGTAGGGCCTGCCGCTTATTGCATCCGTGGGAGC
ACAGCCCTCAATGCGAGGACACCTGGGACCCCTCGGACGAACAGGCGGCG
AGGCGTTTCGTGGCGGCGGTGGGTGCGGCGCTCGGCGGCGCTCGGCGCGC
TTACGAGACGCGAGTGGCGCCCCCGACAGACTCAGAGTGTAGCTTCAGTT
TGGAAACCCGCCGCTGCGAGCCACAAGATCTGTGTCGGCTGCGGGCACGG
CTGGGTCGAGAATGCCAAAGAATTTGTGCTCACGAAAACAGTACCACCTA
CGGACGCCCAATGCGACTTCAACTGGAGCCGCCTACGCTGCGAACCCAAG
TGTGTGTGCGGTATGCGCCTACGCTTCGGAGATTACACGCCTGGTAGGGC
CTGCCGCTTATTGCATCCGTGGGAGCACAGCCCTCAATGCGAGGACACCT
GGGACCCCTCGGACGAACAGGCGGCGAGGCGTTTCGTGGCGGCGGTGGGT
GCGGCGCTCGGCGGCGCTCGGCGCGCTTACGAGACGCGAGTGGCGCCCCC
GACAGACTCAGAGTGTAGCTTCAGTTTGGAAACCCGCCGCTGCGAGCCAC
AAGATCTGTGTCGGCTGCGGGCACGGTGGGGAGACTTGACGTTGAGCAGC
GCATGCAGGCTCACCAAGAACAAGGATGAAAATTCTACAGCAATACCAAA
CAAGATGAGAGCCAGGCCTTTGTAATGGGGAGACTTGACGTTGAGCAGCG
CATGCAGGCTCACCAAGAACAAGGATGAAAATTCTACAGCAATACCAAAC
AAGATGAGAGCCAGGCCTTTGTAA
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