mRNA_H-paniculata_contig3396.9067.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: D7FV40_ECTSI (Valine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FV40_ECTSI)

HSP 1 Score: 1672 bits (4329), Expect = 0.000e+0
Identity = 827/1050 (78.76%), Postives = 914/1050 (87.05%), Query Frame = 3

Query:   51 MVLRDPPSLATPNVVKAAAATKATLMLLLTRSVVARSFSS--LAFVPHGVSLGSSTTTASACRRLSIAARPSPAHPRRSPPIRAGWQRRSLGYGPAIVAGTTPRRWIKTTVSASAAVAEPAAVEG-IRPPSIVDDNGDMATHYDFVSVEKKMYKWWEAQGYFKPGGDPTKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAAGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGNLKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKG-DGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQGEALMVAPWPKVEEEVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPDGQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADLD 3188
            MV RDP   ATP+V KAAAATKATLMLLLTRSV  RSFS+   +FVP      S+    S  RRLS         P        G    ++  G        PRRWI+ + +A+AA A+ A   G +RPPSIVDDNGDMATHYDF+SVE+ MY WWE +GYFKPGGDP KKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRG+PTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFL KVW+WKEDKGGYIVRQMRRLGASADWSRERFTLD +M  AV EAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEY+EE GKLYYFKYMVAGGK+GEHL+VATTRPETILGDTAVCVHPED R+KHLVGGKV+VPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKR SLQTINLM KDG M E AG+YAGQDRF+CR+N+W DME AGL LK EP+QQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAV++ +IQILPERF KVWHNWLDGIHDWC+SRQLWWGHQIPVWYA+GHDGYFVARSE DAR QAT AGV  DV L+RDPDVLDTWFSSGLWPFA+VGWPQ EG    +L  FYPAA LETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKD  GMKMSKTKGNVVDPI+TMD +GTDALRYTLVTGVTPGQDVPLSM+RV+TNMFFANKLWNAGRFLLGNLK     +REALAV+GPM+A ELE+L+LPERYI+S+CH+LVE VT GL+ Y+MGDAG+NIYEFLWDEYADWYIEASKTR+GSFA G DGE  + + +++RRTLVYVFD+CLRLLHPFMPF+TEALWQQLPR GEALMVAPWPKV++  LAVD  AI  F ++Q+LVRS+RNAKAEY+VEPGKKIPA VQVS++ALRS+++KE AALCLLGRLDPE L V+G+  PP+GQ VHLV+QDG++VYLPMAGLVDK+KER+RL KQA KL KDME+L+ RL GSNFVEKAPAKVVE+VRA+L+AQ++QLKTVQQ+I DLD
Sbjct:    1 MVQRDPGQSATPHVAKAAAATKATLMLLLTRSVAVRSFSTSPASFVP------SAAAVRSPLRRLS---------PWPGLHTLDGGSTGAVATGAGATGHRQPRRWIRASATAAAAEADTAKSGGALRPPSIVDDNGDMATHYDFLSVERSMYAWWEEKGYFKPGGDPKKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGEPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLKKVWEWKEDKGGYIVRQMRRLGASADWSRERFTLDPEMSAAVTEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYSEEDGKLYYFKYMVAGGKEGEHLSVATTRPETILGDTAVCVHPEDERFKHLVGGKVVVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRHSLQTINLMEKDGTMAETAGAYAGQDRFDCRNNMWADMEAAGLALKAEPYQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVQEKDIQILPERFNKVWHNWLDGIHDWCVSRQLWWGHQIPVWYASGHDGYFVARSEEDARKQATDAGVPTDVSLDRDPDVLDTWFSSGLWPFASVGWPQEEGNDGGDLSNFYPAACLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDGNGMKMSKTKGNVVDPIDTMDSYGTDALRYTLVTGVTPGQDVPLSMERVQTNMFFANKLWNAGRFLLGNLKGLGEGEREALAVTGPMTAEELETLALPERYIVSKCHRLVEGVTTGLQGYDMGDAGKNIYEFLWDEYADWYIEASKTRIGSFAAGGDGEEGEARARSSRRTLVYVFDTCLRLLHPFMPFITEALWQQLPRTGEALMVAPWPKVDDAPLAVDELAIGRFEAVQSLVRSVRNAKAEYKVEPGKKIPAVVQVSNKALRSDVIKEVAALCLLGRLDPEGLEVQGLDGPPEGQNVHLVVQDGLEVYLPMAGLVDKDKERARLEKQAGKLTKDMEVLEKRLSGSNFVEKAPAKVVEEVRANLQAQKDQLKTVQQAIVDLD 1035          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A836CET5_9STRA (Valine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CET5_9STRA)

HSP 1 Score: 1334 bits (3452), Expect = 0.000e+0
Identity = 642/933 (68.81%), Postives = 750/933 (80.39%), Query Frame = 3

Query:  399 EPAAVEGIRPPSIVDDNGDMATHYDFVSVEKKMYKWWEAQGYFKPGGDPTKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAAGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGNLKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQGEALMVAPWPKVEEEVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGI-GSPPD---GQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            E AAV    PPSI+D+NG+MA  Y+F +VE ++Y WWE QGYFKPGGDPTK S+VVPMPPPNVTG+LHMGHAM   LQDI+TR+HRMRG+PTL++PG DHAGIATQMLVERAL  EG++R+E+GRE FL++VWQWKE+KGGYI+ QMR LGASADWSRERFTL+ DM  AV EAFVRLHEKGLVY+GDYMVNWSPSLQTAVSDLEVEY+EEMGKLYYF+Y VAG  + + + VATTRPETILGDTAVCVHP+D RYKHL+G  V+VP  GREIP+IAD YVDMEFGTGALKITPGHD NDYEIGKR +L  IN++ KD  +    G YAG DRFECR+ LW DM   GL +K EPH QRVPRSQRGGEVIEPLVS+QWFVK + M ++AL AV   +I I PERFEKVW NWL  IHDWC+SRQLWWGH+IPVWY  G +GYFVARS  DARAQAT+AGVS    L +D DVLDTWFSSGLWPFATVGWPQ EGV  S+ ++FYPAAVLETGYDIIFFWVARMVMMG+ELT +SPF  IYLHGLV+D +G KMSKTKGNV+DP++TM +FG DALRYTLVTG +PGQD+PLS +RVE +  FANKLWNAGR+LLGNLK+  + +  ALAV+GPM A EL  L LPER+I+SRCHQLV  VTAGL  Y+MGD GR IYEFLWDE+ADWYIEASK R+G  +  + ++       ARR LVYVFD+CLRLLHPFMPFVTEALWQQLP  G+AL+VAPWP++ +  L VDA A A F + QALVRSIRNA+AEY+VEP +KI A VQVSD ALR++L  E+AAL LL RLDP  LSV  + G+P D   G+ VH V+QDG++ YLP+AG+VD  KE++RL KQAEK+ KD+  L  RL  S F +KAP  VVE  RA L   Q++L TV  S+  +
Sbjct:   70 EAAAVAQCGPPSILDENGEMAKQYNFAAVEGELYAWWERQGYFKPGGDPTKPSFVVPMPPPNVTGHLHMGHAMFVALQDIMTRFHRMRGEPTLWVPGTDHAGIATQMLVERALREEGVERRELGREKFLERVWQWKEEKGGYIINQMRSLGASADWSRERFTLEPDMSAAVVEAFVRLHEKGLVYKGDYMVNWSPSLQTAVSDLEVEYSEEMGKLYYFQYRVAGASESDFIPVATTRPETILGDTAVCVHPDDERYKHLIGKSVVVPTQGREIPVIADTYVDMEFGTGALKITPGHDVNDYEIGKRHALPIINILNKDATINAAGGPYAGLDRFECRERLWSDMAAQGLVIKAEPHLQRVPRSQRGGEVIEPLVSAQWFVKMDRMASRALDAVATKDITIFPERFEKVWQNWLGNIHDWCVSRQLWWGHRIPVWYPEGQEGYFVARSVEDARAQATAAGVSESTPLHQDEDVLDTWFSSGLWPFATVGWPQQEGVSGSDYERFYPAAVLETGYDIIFFWVARMVMMGLELTDQSPFKVIYLHGLVRDGEGQKMSKTKGNVIDPLDTMAQFGADALRYTLVTGASPGQDIPLSQERVEASRNFANKLWNAGRYLLGNLKDVPSAELAALAVTGPMGAYELARLPLPERHIVSRCHQLVARVTAGLGGYDMGDTGRLIYEFLWDEFADWYIEASKVRMGDRSSAEAQT-------ARRVLVYVFDTCLRLLHPFMPFVTEALWQQLPHAGDALIVAPWPQMGDAPLPVDAAATARFEAFQALVRSIRNARAEYKVEPARKISANVQVSDAALRADLTAEAAALALLARLDPAALSVSAMDGAPGDAASGRAVHCVVQDGLEAYLPLAGMVDMAKEKARLAKQAEKIQKDIAALSGRLNSSGFADKAPPAVVEATRAQLVEMQDKLATVLASMESM 995          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A7S3XNU9_HETAK (Valine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XNU9_HETAK)

HSP 1 Score: 1258 bits (3256), Expect = 0.000e+0
Identity = 611/929 (65.77%), Postives = 731/929 (78.69%), Query Frame = 3

Query:  423 RPPSIVDDNGDMATHYDFVSVEKKMYKWWEAQGYFKPGGDPTKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDG---EHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAAGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGNLKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQGEALMVAPWPK-VEEEVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGI--GSPPDGQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADLDA 3191
            +PPSI++D G++A  YDF  VE  +Y+WWE QGYFKP  D   +SYV+PMPPPNVTG LHMGHAM   LQDIL R+HRM+G PTL+LPG DHAGIATQ+LVERALA EG  R+E+GREAFL++VW WKE+KGG I  QMRRLGASADWSRERFTL+ +M  AV EAFV LHE+GL+Y+GDYMVNWSP LQTAVSDLEVEY+EE GKLYYFKYM+A   +G   +HL VATTRPETILGDTAVCVHPED RYKHL+G  V+VP  GREIPIIAD+YVDMEFGTGALKITPGHDPNDYE+GK+ +L TIN+M +D  + E  G YAG DR  CR+ LW DM  AGL LK +PH QRVPRSQRGGEVIEP+VS+QWF +  GM  K+L AV  GEI+I+PERFEK W+NWL+ IHDWCISRQLWWGH+IPV+YA GHDGYFVARS+ +A AQA +A     V L +D DVLDTWFSSGLWPFATVGWP  E   + + ++FYPA  LETGYDI+FFWVARMVMMGIE TGK+PF+ IY+HGLV+D+ G KMSKTKGNV+DPI+T++K+GTDALRY+LVTGVTPGQDVPLSM++VE+N  FANKLWNAGRF+LGNLK+  AE+R ALAV GPMSA EL +L LPE++I+S CH+L  DVTA L+AYN+GDAG  IY+FLWDEYADWYIE+SKTR+ +           +   ARR LVYVFD+CLRLLHPFMP+VTE LWQ+LPR G+ALMVAPWPK   E  L V A A+  + +LQALVR++RNA+AEY V+ G+K+ A VQV+D  LR+EL  E+AAL LLGRLD E LSV  +   SP + + V LV+QDGV+ YLP+AG+VD  KE+ RL KQ              L  S F +KAP +V+E  R     ++EQ+ T+Q SI +++A
Sbjct:   73 KPPSILED-GELAKQYDFARVEAPLYEWWEQQGYFKPN-DKEGESYVLPMPPPNVTGRLHMGHAMFVALQDILARFHRMKGGPTLWLPGTDHAGIATQLLVERALAEEGTSRQELGREAFLERVWAWKEEKGGAITGQMRRLGASADWSRERFTLEPEMSAAVTEAFVTLHERGLIYKGDYMVNWSPHLQTAVSDLEVEYSEEEGKLYYFKYMLADPAEGGGADHLPVATTRPETILGDTAVCVHPEDPRYKHLIGKTVVVPTQGREIPIIADEYVDMEFGTGALKITPGHDPNDYELGKKHALPTINIMNRDASINEAGGPYAGLDRIACRERLWADMGAAGLVLKADPHTQRVPRSQRGGEVIEPMVSTQWFCRMGGMAEKSLEAVRSGEIKIIPERFEKTWYNWLENIHDWCISRQLWWGHRIPVYYAEGHDGYFVARSQEEAEAQAKAALGVDSVALRQDDDVLDTWFSSGLWPFATVGWPNTE---SEDFKRFYPAKCLETGYDILFFWVARMVMMGIEFTGKAPFEVIYMHGLVRDASGQKMSKTKGNVIDPIDTLEKYGTDALRYSLVTGVTPGQDVPLSMEKVESNRNFANKLWNAGRFILGNLKDLPAEERAALAVRGPMSAEELAALPLPEQHIVSLCHELANDVTASLDAYNLGDAGSKIYQFLWDEYADWYIESSKTRMRTD----------QAAMARRVLVYVFDTCLRLLHPFMPYVTEVLWQELPRAGDALMVAPWPKPAGEAALPVAAGAVRRYRALQALVRAVRNARAEYNVDQGRKVEAAVQVADPVLRAELTAEAAALALLGRLDAEKLSVGELTGASPYEEKAVRLVVQDGVEAYLPLAGMVDFAKEKKRLEKQLXXXXXXXXXXXXXLNSSGFADKAPPEVLEATRQQAAEKREQVATIQASIKEIEA 986          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A7S4W3F4_9STRA (Valine--tRNA ligase n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4W3F4_9STRA)

HSP 1 Score: 1174 bits (3037), Expect = 0.000e+0
Identity = 575/928 (61.96%), Postives = 708/928 (76.29%), Query Frame = 3

Query:  468 YDFVSVEKKMYKWWEAQGYFKPGGDP-----TKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLS-GREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYA--AGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQG-------EALMVAPWPKVEEEV-LAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPDG-----QTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            Y+  + E  +YKWWE  G F P         TK+ YV+PMPPPNVTG LHMGHA+   LQD+L R+HRMRGKP L+LPG DHAGIATQ+ VE+AL AEG  R+E+GR+ FL + WQ+KE++GG+I RQ+R LGASADW+RERFT+D D+ EAV EAFVRLHEKGLVYRG+YMVNW+P LQTAVSDLEVEY++E GKL+YFKYMV GG+  E L VATTRPETI+GDTAVCVHPED RYKHLVG K IVP+S GR IPIIAD+YVDMEFGTGALKITPGHDPNDY IGK+  L+ IN+M KDG M  EAG Y G DRF+CR+ LW+DME  GL +KVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKAL AVEDG+++I+P+RFEK+W+NWL  IHDWC+SRQLWWGH+IPVWY   +G   Y VAR+E +AR QA   G   DV L ++ DVLDTWFSSGLWPFATVGWP  EG ++S+L +FYP   LETGYDI+FFWVARM MMG+ELTGK+PFD IYLHGLV+ + G KMSKTKGNVVDP++T+ ++G D+LRY+LVTGVTPGQD+PL+M+++E N  FANKLWN  +F+  N LK    E+ + L V+GP+   E E LSLPERYI+S+CH LV  VT+ +E Y +G AG  +YEFLWDE+ADWYIE SKTR+   A G GE    + K ARR LVY+ D+ LRLLHP+MP+VTE LW  +PR          ALM+A WP++++EV L    +A++SF S QAL RSIRNA+AEY VEPGK+I A + V+  +L+ E+  E  +L  L +LDPE + V   GS         ++V LV+QDGV+ YLP++GL+D  KER RL KQ+EK+ K+++ L  RL    FV+KAPA VV+K + +L   ++Q   ++ S+  L
Sbjct:  119 YNPSAFESHIYKWWEDAGCFTPDAKQSKECNTKQPYVLPMPPPNVTGRLHMGHAIFVALQDVLARFHRMRGKPVLWLPGTDHAGIATQLQVEKALIAEGTTREEVGRDEFLKRTWQYKEEQGGHITRQLRSLGASADWTRERFTMDADLSEAVVEAFVRLHEKGLVYRGEYMVNWAPMLQTAVSDLEVEYSDEEGKLFYFKYMVEGGE--EFLPVATTRPETIMGDTAVCVHPEDTRYKHLVGKKAIVPMSNGRTIPIIADEYVDMEFGTGALKITPGHDPNDYAIGKKFDLEIINIMNKDGSMNGEAGQYEGLDRFDCREKLWEDMEGDGLVIKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALKAVEDGDMKIVPQRFEKIWNNWLTDIHDWCVSRQLWWGHRIPVWYVGESGESEYIVARNEGEARKQAVEKGHPEDVILRQEEDVLDTWFSSGLWPFATVGWPFTEGEEDSDLARFYPGTCLETGYDILFFWVARMAMMGLELTGKAPFDVIYLHGLVRAADGSKMSKTKGNVVDPLDTVAEYGADSLRYSLVTGVTPGQDIPLNMEKIEANRNFANKLWNCCKFVTDNALKGADEEEMKTLGVNGPIGKEEFEKLSLPERYIVSKCHSLVASVTSDIENYQLGAAGSKVYEFLWDEFADWYIEISKTRLYEGAGGGGEE---EAKAARRVLVYILDTSLRLLHPYMPYVTEQLWHHIPRPSATEDQVAHALMLADWPQMDDEVPLTTSEEAVSSFESFQALTRSIRNARAEYNVEPGKRISATI-VAKGSLKDEISAEVKSLVALAKLDPEQVIVVEAGSDEAKAAAAVESVQLVVQDGVEAYLPLSGLIDPVKERKRLEKQSEKIEKEIQKLAGRLQSKGFVDKAPAAVVDKAKNELAELEDQAAKIKSSLEAL 1040          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A1Z5K4B9_FISSO (Valine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K4B9_FISSO)

HSP 1 Score: 1162 bits (3005), Expect = 0.000e+0
Identity = 566/924 (61.26%), Postives = 707/924 (76.52%), Query Frame = 3

Query:  468 YDFVSVEKKMYKWWEAQGYFKPGGDPT--KKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSG-REIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYA--AGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQG------EALMVAPWPKVEE-EVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPP-----DGQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            Y+  S E+++YKWWE+ G FKP       K  YV+PMPPPNVTG LHMGHA+   LQD+L R+HRMRG+P L+LPG DHAGIATQ+ VE+ L AEG  R+E+GRE FL +VW++KE++GG+I RQ+R LGASADWSRERFT+D  + EAV EAFVRLHEKGLVYRG+YMVNW+P L+TAVSDLEVEY+EE GKLYYFKYMV G +  E L VATTRPETI+GDTAVCVHPED RYKHLVG KV+VP+SG R IPIIAD+YVDMEFGTGALKITPGHDPNDY +GK+  L  IN+M +DG M   AG Y GQDRFECR++LW DME  GL +KV+PH QRVPRSQRGGE+IEPL+SSQWFVKTEGMGAKAL AV+DG+IQI+P+RF+K+W NWL  IHDWC+SRQLWWGH+IPVWY   +G   Y VAR+E +AR +A + G +ADV L ++ DVLDTWFSSGLWPFATVGWPQ+EG  +++L +FYPA  LETGYDI+FFWVARMVMMG+ELTG+SPF  IYLHGLV+ + G KMSKTKGNV+DP++T+ ++G D+LRY+LVTGVTPGQD+PL+M++VE+N  FANKLWN  +F+ GN LK     + ++L V GP+   E E ++LPERYIIS+CH LV +VT  LE Y +G AG  +YEFLWD++ADWYIE SKTR+    +G G  ++ + K ARR LVYV D+ L+LLHP+MP+VTE LW  LPR         ALM++ WP++++ + L  D  ++  F   QAL R IRNA+AEY VEPGK+I A V  S + ++  + KE  +L  L +LDP+ ++V   GS         ++V LV+Q+GV+ YLP++GL+D  KER RL KQ+EKL K++E L  RL    F +KAPA VVEK RA+L   ++Q   V  S+  L
Sbjct:   87 YNPSSFEEEIYKWWESSGCFKPADSSKTDKPPYVLPMPPPNVTGRLHMGHAIFVALQDVLARFHRMRGRPVLWLPGTDHAGIATQLQVEKLLRAEGTTREEVGREEFLKRVWEYKEEQGGHITRQLRSLGASADWSRERFTMDPSLSEAVAEAFVRLHEKGLVYRGEYMVNWAPQLKTAVSDLEVEYSEEQGKLYYFKYMVDGSE--EFLPVATTRPETIVGDTAVCVHPEDERYKHLVGKKVVVPMSGGRTIPIIADEYVDMEFGTGALKITPGHDPNDYTLGKKFDLPIINVMNRDGSMNANAGKYEGQDRFECRESLWADMEKEGLVIKVDPHNQRVPRSQRGGEIIEPLISSQWFVKTEGMGAKALKAVQDGDIQIVPQRFDKIWSNWLTDIHDWCVSRQLWWGHRIPVWYVGESGDAEYIVARNEAEAREKAIAQGHTADVVLRQEEDVLDTWFSSGLWPFATVGWPQDEGKPDTDLSRFYPATCLETGYDILFFWVARMVMMGLELTGESPFRVIYLHGLVRAADGSKMSKTKGNVIDPLDTVAEYGADSLRYSLVTGVTPGQDIPLNMEKVESNRNFANKLWNCCKFVTGNALKGADQAELDSLGVDGPILKEEFEKMALPERYIISKCHTLVAEVTEDLEKYQLGSAGSKVYEFLWDQFADWYIEISKTRL---YEGAGGGNEEEAKIARRVLVYVLDTSLKLLHPYMPYVTEQLWHHLPRPKAVGQATNALMLSNWPQMDDAQPLVTDKSSVNKFECFQALTRRIRNARAEYNVEPGKRISAVVIASGD-IKKAIEKEIKSLVALAKLDPDQVAVYEAGSEEAKTAASVESVQLVVQEGVEAYLPLSGLIDPVKERKRLEKQSEKLSKEIEKLAGRLQSKGFTDKAPADVVEKARAELAELEDQAAKVNASLGAL 1004          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A7S4MJV7_9STRA (Valine--tRNA ligase n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4MJV7_9STRA)

HSP 1 Score: 1157 bits (2992), Expect = 0.000e+0
Identity = 567/931 (60.90%), Postives = 698/931 (74.97%), Query Frame = 3

Query:  468 YDFVSVEKKMYKWWEAQGYFKP---------GGDPTKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSG-REIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAAGHDG-YFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKN-SELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQ------GEA---LMVAPWPKVEE-EVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPDG-----QTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSI 3176
            YD  S E  +Y+WWE  G F P         G    KK YV+PMPPPNVTG LHMGHAM   LQD L R+HRMRG+P L+LPG DHAGIATQ+ VE+ L AEG  R+++GRE FL +VW++KE++GG+I RQ+R LGASADWSRERFT+D D+ EAV+EAFVRLH+KGLVYRG+YMVNW+P LQTAVSDLEVEY+EE GKLYYFKYMV GG + ++L VATTRPETI+GDTAVCVHPED RYKHLVG + +VPLSG R IPIIADDYVDMEFGTGALKITPGHDPNDY +GK+  L  +N+M KDG M   AGSY G DRFECR+ LW DME  GL +K EPH QRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKAL AVEDG IQI+P+RFEK+W+NWL  IHDWC+SRQLWWGH+IPVWY    +  Y VAR+  +AR +A++ GV  DV+L ++ DVLDTWFSSGLWPFAT GWP  EG++  ++  +FYP   LETGYDI+FFWVARM MMG+ELTG++PFD +YLHGLV+ S G KMSKTKGNV+DP++T+ ++G D+LRY+LVTGVTPGQD+PL+M+++E N  FANKLWN  +F+  N LK+ S ++   L V GPM+  EL SLSLPERYI+S+CH LV  VT+ +E+Y++G AG  +YEFLWD+YADWYIE SKTR+   A G G       + ARR LVY+ D  LRLLHP+MP+VTE LW  LPR       G A   LM+A WP++++   L  D  A+ +F S QAL RSIRNA++EY VEPGKKI A V  +   L+ E+ +E  +L  L +LDP+++SV   G+          +V LV+QDGV+ YLP++GL+D  KE+ RL +Q EKL K++  L  RL    FV+KAP  VV K R +L   ++Q   +Q S+
Sbjct:   43 YDPASFETDIYRWWEGTGCFDPDASRSEEHRGTPDEKKPYVLPMPPPNVTGRLHMGHAMFVALQDALARFHRMRGRPVLWLPGTDHAGIATQLQVEKQLIAEGTTREQVGREEFLRRVWEYKEEQGGHITRQLRSLGASADWSRERFTMDPDLSEAVSEAFVRLHDKGLVYRGEYMVNWAPMLQTAVSDLEVEYSEEEGKLYYFKYMVDGGTEEDYLPVATTRPETIVGDTAVCVHPEDERYKHLVGKECVVPLSGGRTIPIIADDYVDMEFGTGALKITPGHDPNDYALGKKYDLPIVNVMNKDGSMNGNAGSYDGLDRFECREKLWSDMEGEGLVIKAEPHTQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALKAVEDGSIQIVPQRFEKIWNNWLTDIHDWCVSRQLWWGHRIPVWYVGESESEYVVARNADEAREKASAMGVPDDVELNQEEDVLDTWFSSGLWPFATTGWPAAEGIEGQTDYNRFYPGTCLETGYDILFFWVARMAMMGLELTGRAPFDVVYLHGLVRASDGTKMSKTKGNVIDPLDTVAEYGADSLRYSLVTGVTPGQDIPLNMEKLEANRNFANKLWNCCKFVTDNALKDASDDEMANLGVDGPMTQEELNSLSLPERYIVSKCHDLVASVTSDIESYSLGAAGSKVYEFLWDQYADWYIEISKTRLYEGAGGGGVDES---RAARRVLVYILDVSLRLLHPYMPYVTEQLWHHLPRSINTKAGGSAPHSLMLADWPQLDDGSPLPTDPDAVNAFQSFQALTRSIRNARSEYNVEPGKKISATVVANAGPLKDEIEREIKSLVALAKLDPDSVSVVESGTDEAKAASAVDSVQLVVQDGVEAYLPLSGLIDPVKEKKRLERQKEKLGKEIVKLAGRLSSKGFVDKAPEVVVNKARDELAELEDQAAKIQSSL 970          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A7S3DVF3_9STRA (Valine--tRNA ligase n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S3DVF3_9STRA)

HSP 1 Score: 1152 bits (2981), Expect = 0.000e+0
Identity = 568/938 (60.55%), Postives = 702/938 (74.84%), Query Frame = 3

Query:  435 IVDDNGDMATHYDFVSVEKKMYKWWEAQGYFKPGGDPT----KKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSG-REIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYA--AGHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQ--GE-----ALMVAPWPKVEEEV-LAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPDG-----QTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            I++  G +   YD  S E ++Y+WWE  G F+P         +K YV+PMPPPNVTG LHMGHA+   LQD+L R+HRMRG+P L+LPG DHAGIATQ+ VE+ L AEG  R+E+GRE FL +VW +KE++GG+I RQ+R LGASADWSRERFT+D+D+ EAV EAFVRLHEKGLVYRG+YMVNW+P L+TAVSDLEVEY+EE GKLYYFKY V G    E L VATTRPETI GDTAVCVHPED RYKHLVG  V+VP+ G R IPIIAD+YVDMEFGTGALKITPGHDPNDY +GK+  L  IN+M KDG M   AG Y G DRFECR+ LW DM+ AGL ++ +PH QRVPRSQRGGEVIEP+VSSQWFVKTEGMGAKAL AV+ G+I+I+P+RF+K+W+NWL+ IHDWC+SRQLWWGH+IPVWY   +G   Y VAR+E +AR +A   G  ADV L ++ DVLDTWFSSGLWPFATVGWPQNEGV+ S+L +FYP + LETGYDIIFFWVARMVMMG+ELTG+SPF+ IYLHGLV+ + G KMSKTKGNV+DP++T+ ++G D+LRY+LVTGVTPGQD+PL+M++++ N  F NKLWN  +F+ GN L++   E    + VSGP+   E +SLSLPERYIIS+CH LVE VT+ +E Y++G AG  +YEFLWD YADWYIE SKTR+   A G GE+   ++K  RR LVYV D+ LRLLHP+MP++TE LW  LPR   GE     +LM+A WP++++E  L   A A+  F   QAL RSIRNA+AEY VEPGK+I A + V+   +R EL KE  +L  L +LDPE ++V   GS          +V LVI+DGV+ YLP++GL+D +KER RL KQ E        L  RL    FV+KAP  VV+K + DL   ++Q   V+  +  L
Sbjct:  112 ILNKKGGLDGSYDPASFESEVYQWWENSGCFQPDAKQKPQVGEKPYVLPMPPPNVTGRLHMGHAIFVALQDVLARFHRMRGQPVLWLPGTDHAGIATQLQVEKQLLAEGTTREEVGREEFLRRVWDYKEEQGGHITRQLRSLGASADWSRERFTMDQDLSEAVAEAFVRLHEKGLVYRGEYMVNWAPLLKTAVSDLEVEYSEEEGKLYYFKYNVEGSD--EFLPVATTRPETICGDTAVCVHPEDERYKHLVGKTVVVPMGGGRSIPIIADEYVDMEFGTGALKITPGHDPNDYALGKKYDLPIINIMNKDGSMNSNAGEYDGLDRFECREKLWSDMDEAGLVIEAKPHTQRVPRSQRGGEVIEPMVSSQWFVKTEGMGAKALQAVKGGDIKIVPQRFDKIWYNWLEDIHDWCVSRQLWWGHRIPVWYVGESGESEYVVARNEKEAREKAIEKGHDADVVLRQEEDVLDTWFSSGLWPFATVGWPQNEGVEGSDLSRFYPGSCLETGYDIIFFWVARMVMMGLELTGESPFNVIYLHGLVRAADGSKMSKTKGNVIDPLDTVAEYGADSLRYSLVTGVTPGQDIPLNMEKIKANRQFGNKLWNCCKFVTGNALRDMDEESLAEIGVSGPIMQEEFDSLSLPERYIISKCHTLVEGVTSDIENYSLGAAGSKVYEFLWDHYADWYIEISKTRLYEGAGGGGEA---EMKATRRVLVYVLDTSLRLLHPYMPYITERLWHHLPRSKAGEDQAAHSLMLADWPQMDDESPLVTSADAVDQFECFQALTRSIRNARAEYNVEPGKRISAVI-VASGGMREELEKEVKSLVALAKLDPEQVAVLEAGSDEAKAATAVDSVQLVIRDGVEAYLPLSGLIDPDKERKRLEKQDEXXXXXXXXLAGRLKSKGFVDKAPEAVVDKAKQDLAELEDQAAKVKAGLEAL 1043          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A448Z0P4_9STRA (Valine--tRNA ligase n=2 Tax=Pseudo-nitzschia TaxID=41953 RepID=A0A448Z0P4_9STRA)

HSP 1 Score: 1152 bits (2980), Expect = 0.000e+0
Identity = 564/929 (60.71%), Postives = 700/929 (75.35%), Query Frame = 3

Query:  465 HYDFVSVEKKMYKWWEAQGYFKPGGDPTK--------KSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPL-SGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAA--GHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQ--GE-----ALMVAPWPKVEEEV-LAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPP-----DGQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSI 3176
            +Y     E  +YKWWE+ G F+P    TK        K YV+PMPPPNVTG LHMGHA+   LQD+L R+HRMRG+P L+LPG DHAGIATQ+ VE+ L AEG  R+E+GRE FLD+VW +K ++GG+I RQ+R LGASADW+RERFT+D D+ EAV+EAFVRLH+KGL+YRG+YMVNW+P L+TAVSDLEVEYTEE GKLY+FKYMV G  DGE L VATTRPETI+GDTAVCVHPED RYK LVG + +VP+ +GR IPIIAD+YVDMEFGTGALKITPGHDPNDY IGK+  L  IN+M KDG + E AGSY G DRF+ R+ LWKDME   LT+KV+PH QRVPRSQRGGE+IEPLVSSQWFVKTEGMGAKAL AVEDG+I+I+P+RF+K+W+NWL  IHDWCISRQLWWGH+IPVWY    G   + VAR+E +AR++A   G   DV L ++ DVLDTWFSSGLWPFATVGWP NEG  NS+L +FYP   LETGYDI+FFWVARMVMMGIELTG+SPF  IYLHGLV+ + G KMSKTKGNV+DP++T+  +G D+LRY+LVTGVTPGQD+PL+M+++E N  F NKLWN+ +F+ GN LK+    + E L V+GPMS  E + L LPE+YI+S+ H+LV  VT  +E Y +G AG  IYEF+WD+YADWYIE SKTR+    +G+G    ++ K +RR LVYV D  LR+LHP+MP+VTE LW  LPR   GE     ALM++ WP++++   L  +  ++++F   QA+ RSIRNA+AEY VE GKKI A V      L+  +  E  +L LL RLDPE   V   GS       + ++V LV+QDGV+V+LP++GL+D EKER+RL KQ  KL  +++ L  RL    FV+KAPA VV+K +A+L   + Q K VQ S+
Sbjct:  128 NYAPTEFENDIYKWWESAGCFQPDAKQTKEESDKNGRKPYVLPMPPPNVTGRLHMGHAIFVALQDVLARFHRMRGRPVLWLPGTDHAGIATQLQVEKQLIAEGTSREEVGREEFLDRVWTYKAEQGGHITRQLRSLGASADWTRERFTMDSDLSEAVSEAFVRLHDKGLIYRGEYMVNWAPMLKTAVSDLEVEYTEEEGKLYFFKYMVEGS-DGEFLPVATTRPETIMGDTAVCVHPEDERYKDLVGKQAVVPMGNGRTIPIIADEYVDMEFGTGALKITPGHDPNDYAIGKKFDLPIINIMNKDGSLNENAGSYNGLDRFDAREALWKDMEKESLTIKVDPHTQRVPRSQRGGEIIEPLVSSQWFVKTEGMGAKALKAVEDGDIEIIPKRFDKIWNNWLTDIHDWCISRQLWWGHRIPVWYVGETGESEFIVARNEKEARSKAVENGHPEDVVLRQEEDVLDTWFSSGLWPFATVGWPANEGDVNSDLARFYPGTCLETGYDILFFWVARMVMMGIELTGESPFSVIYLHGLVRAADGSKMSKTKGNVIDPLDTVADYGADSLRYSLVTGVTPGQDIPLNMEKIEANRNFCNKLWNSCKFVTGNALKDADEAEMEELGVTGPMSQEEFDKLLLPEKYIVSKAHELVASVTEDIEKYQLGAAGSKIYEFMWDQYADWYIEISKTRL---YEGNGGGDIVEAKASRRVLVYVLDIILRVLHPYMPYVTEKLWHHLPRAKAGENQAAHALMLSDWPQMDDAFPLVTNHDSVSTFECFQAVTRSIRNARAEYNVEQGKKISAIVVAPAGTLKDAIEAELKSLVLLARLDPEDAKVVEAGSDDAKAANNDESVQLVVQDGVEVFLPLSGLIDPEKERARLTKQQGKLEIEIQKLAGRLSSKGFVDKAPAAVVDKAKAELAELESQAKMVQSSL 1052          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: B7G3Y1_PHATC (Valine--tRNA ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G3Y1_PHATC)

HSP 1 Score: 1148 bits (2970), Expect = 0.000e+0
Identity = 566/932 (60.73%), Postives = 691/932 (74.14%), Query Frame = 3

Query:  468 YDFVSVEKKMYKWWEAQGYFKPGG---------DPTKKS-YVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKDGEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSG-REIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAA--GHDGYFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGN-LKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQ-------GEALMVAPWPKVEEEVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPDGQ-----TVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            Y+  S E  +Y+WWE  G F+P           D T  + YV+PMPPPNVTG LHMGHA+   LQD+L R+HRMRG+P L+LPG DHAGIATQ+ VE+ L AEG  R+E+GR+ FL +VW +KE++GG+I  Q+R LGASADWSRERFT+D+D+ +AV EAF RLHEKGLVYRG+YMVNW+P LQTAVSDLEVEY+EE GKLYYFKYMV G +  E + VATTRPETI GDTAVCVHPED RYKHLVG  ++VP+SG R +P+IAD+YVDMEFGTGALKITPGHDPNDY +GK+  L  IN+M KDG M   AG Y G DRFECR  LW DME  GL +K +PH QRVPRSQRGGE+IEPLVSSQWFVKTEGMGAKAL AVEDG+I+I+P+RF+K+W+NWL  IHDWC+SRQLWWGH+IPVWY    G D + VAR+E +AR +A + G SADV L ++ DVLDTWFSSGLWPFATVGWPQNEGVK S+  +F+PA+ LETGYDIIFFWVARMVMMGIELTGKSPF  +YLHGLV+ + G KMSKTKGNV+DP++T+ +FG D+LRY+LVTGVTPGQD+PL+M+++E N  FANKLWN  +F+ GN LK+ S ED  +LAVSGP+   E +SL LPERYIIS+CH LV  VT  +E Y +G AG  +YEFLWD++ADWYIE SKTR+   A G     + +   ARR LVYV D+ LRLLHP+MP+VTE LW  LPR          ALM+A WP++ + VL     A+A F S QAL RS+RNA+AEY VEPGK+I A + V+   L+  + KE  +L  L +LDPE   +   GS    Q     +V +V+QDGV+ +LP++GL+D EKER RL                RL    FV+KAPA VVEK +A+L   ++Q   VQ S+  L
Sbjct:  107 YEPASFESDIYRWWETAGCFQPDAKQKAIDDNDDSTSTAPYVLPMPPPNVTGRLHMGHAIFVALQDVLARFHRMRGRPVLWLPGTDHAGIATQLQVEKLLIAEGTTREEVGRDEFLRRVWMYKEEQGGFITSQLRSLGASADWSRERFTMDDDLSQAVVEAFCRLHEKGLVYRGEYMVNWAPLLQTAVSDLEVEYSEEEGKLYYFKYMVEGSE--EFIPVATTRPETICGDTAVCVHPEDERYKHLVGKALVVPMSGGRTVPVIADEYVDMEFGTGALKITPGHDPNDYTLGKKFDLPIINIMNKDGSMNANAGQYDGLDRFECRQQLWTDMETEGLVIKADPHTQRVPRSQRGGEIIEPLVSSQWFVKTEGMGAKALKAVEDGDIKIVPQRFDKIWNNWLTDIHDWCVSRQLWWGHRIPVWYVGETGEDEFIVARNEKEAREKAVANGHSADVVLRQEEDVLDTWFSSGLWPFATVGWPQNEGVKGSDFDRFFPASCLETGYDIIFFWVARMVMMGIELTGKSPFSVVYLHGLVRAADGSKMSKTKGNVLDPLDTVAEFGADSLRYSLVTGVTPGQDIPLNMEKIEANRNFANKLWNCCKFVTGNALKDLSDEDLASLAVSGPIEQEEFDSLLLPERYIISKCHTLVASVTQDIEKYQLGAAGSKVYEFLWDQFADWYIEISKTRLYEGAGGGDNIEEAQA--ARRVLVYVLDTSLRLLHPYMPYVTEQLWHHLPRADAGPDQAAHALMLANWPQMNDNVLTTSEAAVAQFESFQALTRSVRNARAEYNVEPGKRIAAVI-VARGKLKQAIEKELKSLIALAKLDPEQTLIYEAGSEEARQATQVESVQVVVQDGVEAFLPLSGLIDPEKERLRLEXXXXXXXXXXXXXAGRLQSKGFVDKAPADVVEKAQAELAELEDQAGKVQASLETL 1033          

BLAST of mRNA_H-paniculata_contig3396.9067.1 vs. uniprot
Match: A0A7S4ETN8_CHRCT (Valine--tRNA ligase n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4ETN8_CHRCT)

HSP 1 Score: 1144 bits (2958), Expect = 0.000e+0
Identity = 560/940 (59.57%), Postives = 697/940 (74.15%), Query Frame = 3

Query:  453 DMATHYDFVSVEKKMYKWWEAQGYFKPGGDPTKKSYVVPMPPPNVTGYLHMGHAMGTTLQDILTRYHRMRGKPTLFLPGQDHAGIATQMLVERALAAEGIDRKEIGREAFLDKVWQWKEDKGGYIVRQMRRLGASADWSRERFTLDEDMIEAVNEAFVRLHEKGLVYRGDYMVNWSPSLQTAVSDLEVEYTEEMGKLYYFKYMVAGGKD----GEHLAVATTRPETILGDTAVCVHPEDARYKHLVGGKVIVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRCSLQTINLMTKDGRMGEEAGSYAGQDRFECRDNLWKDMENAGLTLKVEPHQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVEDGEIQILPERFEKVWHNWLDGIHDWCISRQLWWGHQIPVWYAAGHDG------------------YFVARSEHDARAQATSAGVSADVKLERDPDVLDTWFSSGLWPFATVGWPQNEGVKNSELQKFYPAAVLETGYDIIFFWVARMVMMGIELTGKSPFDTIYLHGLVKDSKGMKMSKTKGNVVDPIETMDKFGTDALRYTLVTGVTPGQDVPLSMDRVETNMFFANKLWNAGRFLLGNLKEPSAEDREALAVSGPMSAAELESLSLPERYIISRCHQLVEDVTAGLEAYNMGDAGRNIYEFLWDEYADWYIEASKTRVGSFAKGDGESHDLKVKNARRTLVYVFDSCLRLLHPFMPFVTEALWQQLPRQGEALMVAPWPKVEEEVLAVDAKAIASFGSLQALVRSIRNAKAEYRVEPGKKIPAFVQVSDEALRSELLKESAALCLLGRLDPETLSVEGIGSPPD-------GQTVHLVIQDGVQVYLPMAGLVDKEKERSRLLKQAEKLIKDMEMLKTRLGGSNFVEKAPAKVVEKVRADLKAQQEQLKTVQQSIADL 3185
            DM T Y   SVE+++Y+WWE+ GYFKP GD +K+ +V+ MPPPNVTG LHMGHAM   L+DI+ R+HRMRG PTL+LPG DHAGIATQMLVERAL AEG  R E+GR+AFL +VW+WKE+ GG I  Q+RRLGAS DWSRE+FTL  +++ AVNEAFVRLHEKGLVYRG+YMVNWSP+L TAVSDLEV Y EE G LYYFKYM+A   D    G ++ VATTRPETILGDTAVCVHPED R+KHLVG +V+VP++GR++ +IADDYV M+FGTGALKITP HD NDY IGKR  L  IN++ KD  +    G YA  DR++ R  LW DM+ AGL LK E H QRVPRS R GEVIEPL+S+QWF+K +GM  K L AV  G+ +ILPERFEKV+ NWL+ I DWC+SRQLWWGH+IPVWY  G D                   +FVAR+E +AR +A  A     V+L +D DVLDTWFSSGLWPFATVGWP +     +EL KFYP+AV+ETGYDI+FFWVARM+M+G+ELTG++PF TIYLHGLV+D++G KMSKTKGNVVDPIET++  GTDALR +LVTGVTPGQDVPLS+++V+ N  FANKLWN  RF+L  L +  A+ ++ALAV+  +   EL ++ LPER+++SRCH LV++VT+ L++Y+ G AG+ IY FLWDEYADWYIE SK R+ S   GD E+     + +RR LVYV D+CLRLLHP+MP++TE LWQ+LP +GEALM++ WP+ E +VLA D++A   F S+QALVRS+RNA+AEYRVE  KK+ A V  +D AL   +  E+ A+  LGR+DP    V   G           G  V LV++DG++  LPMA LVD EKER+RL KQ + L+  +E L+TRL    F +KAPA VV K   +L+ QQEQL  V+ S+  L
Sbjct:  121 DMPTQYSPSSVEQRLYQWWESSGYFKPAGDESKQCFVISMPPPNVTGKLHMGHAMFVALEDIMARFHRMRGHPTLWLPGTDHAGIATQMLVERALKAEGQSRVELGRDAFLKRVWEWKEEYGGAITGQIRRLGASCDWSREKFTLQPELVTAVNEAFVRLHEKGLVYRGEYMVNWSPALGTAVSDLEVNYVEEEGTLYYFKYMLADADDASGEGAYIPVATTRPETILGDTAVCVHPEDERFKHLVGKEVVVPMTGRKVRVIADDYVQMDFGTGALKITPAHDINDYAIGKRHDLPLINILNKDATINANGGKYADLDRYDARAQLWSDMDAAGLVLKTEKHTQRVPRSDRSGEVIEPLISTQWFIKMDGMAQKGLDAVRTGQTKILPERFEKVYFNWLENIQDWCVSRQLWWGHRIPVWYVEGVDAADLAPGKGAKDGAKARQTFFVARNEAEAREKA-EAVYGEGVQLRQDEDVLDTWFSSGLWPFATVGWPADNEEAKAELNKFYPSAVMETGYDILFFWVARMMMLGLELTGEAPFHTIYLHGLVRDAQGQKMSKTKGNVVDPIETIEAMGTDALRLSLVTGVTPGQDVPLSLEKVQANRNFANKLWNTARFILIGLDDMPADAKKALAVTSAIDKDELSTMPLPERWVVSRCHALVDEVTSQLKSYDFGPAGQAIYAFLWDEYADWYIEISKRRIAS---GDVEA----AQRSRRVLVYVLDTCLRLLHPYMPYITEELWQKLPHEGEALMISNWPQAEGDVLASDSQAEKQFESIQALVRSVRNARAEYRVEGFKKVAATV-FADAALADAISAEADAVSTLGRIDPAEFKVVEAGQEASETAKAEAGNAVRLVVEDGLEALLPMAALVDAEKERARLTKQEKTLVAAIEKLETRLNSPGFADKAPAAVVAKAEGELREQQEQLLAVRDSLKAL 1051