mRNA_H-paniculata_contig10.8.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig10.8.1
Unique NamemRNA_H-paniculata_contig10.8.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: A0A090C2V0_HYAAE (RxLR effector candidate protein (Fragment) n=3 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=A0A090C2V0_HYAAE)

HSP 1 Score: 162 bits (411), Expect = 2.380e-46
Identity = 81/182 (44.51%), Postives = 118/182 (64.84%), Query Frame = 1
Query:    7 DPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIG-FGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKFQTL 549
            DPC   + A KR+ RYL A P+ G+ F  +  G R+ G+ DADWA D+E R+ST+GYVF +NGG I+W S+KQ+ VA SSTEAEY+A  EA  + V+L+  LG +G      +  +FEDN+ +I L+     H R KHI +R+H +RE +  G + L+Y    D LAD++ K ++ V+F++L
Sbjct:   68 DPCPTHWQALKRVLRYLQATPNHGLEFT-REEGSRICGYTDADWAGDIESRRSTSGYVFMMNGGCISWKSQKQRTVALSSTEAEYMALSEATKEAVWLKVFLGELGEMTSDEAIKMFEDNQGSIALAKNPEFHKRTKHIDIRYHFVREKVESGEVILEYCSTQDMLADMMTKPIAAVQFESL 248          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: H3GB69_PHYRM (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GB69_PHYRM)

HSP 1 Score: 160 bits (404), Expect = 5.090e-45
Identity = 80/169 (47.34%), Postives = 112/169 (66.27%), Query Frame = 1
Query:   31 ACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVK 537
            A KRI +YL    DLG++F+G + G  L G+ DA+WA D++ R+STTGYVF LNG AI+W S++Q  VA SSTEAEY+A   A  + V+LRQLL  +G  C+G+TT F+DN+  I L+     H R KHI L++H +RE + + V+ L+Y    + +AD   K L+R K
Sbjct:  133 AAKRILKYLKTTQDLGLVFNGGTKGE-LIGYADANWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNATQEAVWLRQLLLDVGCECVGATTFFQDNQGCIALAKNPAYHSRTKHIDLKYHFLREKVEDKVIVLEYKPTDEMIADGFTKALARPK 300          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: A0A151QXQ6_CAJCA (Copia protein n=2 Tax=Cajanus cajan TaxID=3821 RepID=A0A151QXQ6_CAJCA)

HSP 1 Score: 154 bits (388), Expect = 7.140e-44
Identity = 82/183 (44.81%), Postives = 111/183 (60.66%), Query Frame = 1
Query:    1 MEDPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKFQTL 549
            M  P    F   KR+ RYL    D GI +  KS+G ++ GFVD+DWA  ++D KSTTGYVF L  G  +W+S+KQ  VA SS EAEYVAA  A N  +++R++L  +    M  T L+ DN+SAI+++     H R KHI +++H IRE  + G + L Y +  +QLADIL K L   KF  L
Sbjct:   11 MHSPSLTHFGVGKRVLRYLKGTADFGIWYS-KSDG-KVEGFVDSDWAGSIDDSKSTTGYVFSLGSGVFSWNSKKQDVVAQSSAEAEYVAAAAASNQAIWVRKVLTDLSHVQMEPTVLWCDNKSAISIAKNPIQHGRTKHINVKFHAIREAEKNGDVQLMYCRSEEQLADILTKALPSAKFNEL 191          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: H3G656_PHYRM (Uncharacterized protein n=3 Tax=Phytophthora ramorum TaxID=164328 RepID=H3G656_PHYRM)

HSP 1 Score: 153 bits (386), Expect = 2.470e-43
Identity = 76/182 (41.76%), Postives = 115/182 (63.19%), Query Frame = 1
Query:    7 DPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIG-FGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKFQTL 549
            DPC   + A KR+ RYL A P  G+ F  + +   + G+ DADWA D+E R+ST+GY F +NGG I+W S+KQ+ VA SSTEAEY+A  EA  + V+L+  L  +G      +  +++DN+ +I L+     H R KHI +R+H +RE + +G + L+Y+   D LADI+ K ++ V+F+ L
Sbjct:   38 DPCPTHWQALKRVLRYLQATPTHGLEFS-REDSLGMCGYSDADWAGDIESRRSTSGYAFMMNGGCISWRSKKQRTVALSSTEAEYMALSEATQEAVWLKVFLCELGEMASDEAVKIYKDNQGSIALAKNPQFHKRTKHIDIRYHFVREKVEDGQVVLQYISTTDMLADIMTKAITAVQFRVL 218          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: H3GB17_PHYRM (Reverse transcriptase Ty1/copia-type domain-containing protein n=2 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GB17_PHYRM)

HSP 1 Score: 158 bits (399), Expect = 2.510e-43
Identity = 79/169 (46.75%), Postives = 111/169 (65.68%), Query Frame = 1
Query:   31 ACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVK 537
            A KRI +YL    DLG++F+G + G  L G+ DA WA D++ R+STTGYVF LNG AI+W S++Q  VA SSTEAEY+A   A  + V+LRQLL  +G  C+ +TT+F+DN+  I L+     H R KHI L++H +RE + + V+ L+Y    + +AD   K L+R K
Sbjct:  229 AAKRILKYLKTTQDLGLVFNGGTKGE-LIGYADASWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNATQEAVWLRQLLLDVGCECVAATTIFQDNQGCIALAKNPAYHSRTKHIDLKYHFLREKVEDKVIVLEYKPTDEMIADGFTKALARPK 396          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: H3G557_PHYRM (Uncharacterized protein n=3 Tax=Phytophthora ramorum TaxID=164328 RepID=H3G557_PHYRM)

HSP 1 Score: 153 bits (386), Expect = 2.550e-43
Identity = 74/150 (49.33%), Postives = 104/150 (69.33%), Query Frame = 1
Query:   31 ACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKY 480
            A KRI +YL    DLG++F+G + G  L G+ DA+WA D++ R+STTGYVF LNG AI+W S++Q  VA SSTEAEY+A   A  + V+LRQLL  +G  C+G+TT+F+DN+  I L+     H R KHI L++H +RE + + V+ L+Y
Sbjct:   74 AAKRILKYLKTTQDLGLVFNGGTKGE-LIGYADANWAGDLDSRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNATQEAVWLRQLLLDVGCECVGATTIFQDNQGCIALAKNPAYHSRTKHIDLKYHFLREKVEDKVIVLEY 222          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: UPI001C093D0F (LOW QUALITY PROTEIN: secreted RxLR effector protein 161-like n=1 Tax=Alosa sapidissima TaxID=34773 RepID=UPI001C093D0F)

HSP 1 Score: 152 bits (383), Expect = 1.300e-42
Identity = 74/178 (41.57%), Postives = 115/178 (64.61%), Query Frame = 1
Query:    7 DPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKF 540
            DP ++ ++A KRI RYL+    LG+++  K+      G+ DADWA D++DRKS +GY+FK++G A++W S+KQ  V  S+ EAEY+A   A  + V++RQLL  +     G+T ++EDN+SAI ++     H R KHI ++ H IR+ +  G + LK  +  D +AD+L K L++VKF
Sbjct:   58 DPTKQHWTAVKRILRYLNGTQPLGLLYS-KNTEKECIGYSDADWAGDLDDRKSVSGYMFKMSGAAVSWKSKKQSXVLLSTAEAEYMALASAAQEAVWMRQLLSDLKNCPAGATVIYEDNQSAICMARNPQFHGRAKHIDIKHHFIRDQVASGAVDLKDCKTDDMIADMLTKGLTQVKF 234          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: A0A4Y9ZSL8_9AGAM (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Hericium alpestre TaxID=135208 RepID=A0A4Y9ZSL8_9AGAM)

HSP 1 Score: 154 bits (389), Expect = 1.440e-42
Identity = 79/169 (46.75%), Postives = 104/169 (61.54%), Query Frame = 1
Query:   31 ACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVK 537
            A    F+YL    D  ++FDGK     L G+VDADWA DV DR+S +GY F L GGAI+WSS+KQ   A SSTEAEY+ A  A  +  +LR  L  IG  C  +T L  DN+SA+ ++     H R KHI +R+H +RE +  G + L+YV  GDQ+AD+L K L + K
Sbjct:  151 ALVHAFKYLRGTADYQLVFDGKKPWE-LTGYVDADWAGDVNDRRSISGYTFLLAGGAISWSSKKQNTTALSSTEAEYIVATHAAKEATWLRTFLSKIGRPCKAATLLLMDNQSAMAIAWNPAFHARTKHIDMRYHFLREKVEAGAINLQYVPTGDQVADVLMKGLVKAK 318          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: A0A2P4XPH2_9STRA (RxLR effector candidate protein (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4XPH2_9STRA)

HSP 1 Score: 151 bits (382), Expect = 1.510e-42
Identity = 76/175 (43.43%), Postives = 111/175 (63.43%), Query Frame = 1
Query:   31 ACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKFQTLIN 555
            A KRI +YL    D+G++F+G   G  L G+ DA+WA D++ R+STTGYVF LNG AI+W S++Q  VA SSTEAEY+A   A  + V+LRQLL  +G+   G+TT+++DN+  I L+     H R KHI +++H +RE +    + L+Y    + +AD   K L+R K    +N
Sbjct:   61 AAKRILKYLKTTQDVGLVFNGGIKGE-LVGYADANWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNAIQEAVWLRQLLKDLGYENKGATTIYQDNQGCIALAKNPAYHTRTKHIDIKFHFLREKVESKTVELEYKPTDEMIADGFTKALARDKHNKFVN 234          
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Match: A0A8K1FHK1_PYTOL (Uncharacterized protein n=3 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FHK1_PYTOL)

HSP 1 Score: 161 bits (408), Expect = 1.650e-42
Identity = 84/182 (46.15%), Postives = 116/182 (63.74%), Query Frame = 1
Query:    7 DPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDVEDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYLRQLLGSIGFGC-MGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREWIREGVLALKYVQIGDQLADILPKRLSRVKFQTL 549
            DPC   + A KR+ RYL A P  GI F G S+G  L G+ DADWA D+E R+ST+GYV  LNGG I+W S+KQ+ VA SSTEAEY+A  EA  + V+L+  +  +G     G+ T+FEDN+ AI L+     H R KHI +R+H +RE + +G + L+Y    + LAD++ K L+  +F  L
Sbjct: 1194 DPCPTHWQALKRVLRYLQATPMHGICFSGSSDGE-LIGYSDADWAGDIETRRSTSGYVSVLNGGCISWRSKKQRTVALSSTEAEYMALSEATQEAVWLKSFMCELGEDSGNGALTVFEDNQGAIALAKNPEFHKRTKHIDIRYHFVREKVEDGQVVLEYCPTQEMLADLMTKPLAAAQFDAL 1374          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig10.8.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A090C2V0_HYAAE2.380e-4644.51RxLR effector candidate protein (Fragment) n=3 Tax... [more]
H3GB69_PHYRM5.090e-4547.34Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A151QXQ6_CAJCA7.140e-4444.81Copia protein n=2 Tax=Cajanus cajan TaxID=3821 Rep... [more]
H3G656_PHYRM2.470e-4341.76Uncharacterized protein n=3 Tax=Phytophthora ramor... [more]
H3GB17_PHYRM2.510e-4346.75Reverse transcriptase Ty1/copia-type domain-contai... [more]
H3G557_PHYRM2.550e-4349.33Uncharacterized protein n=3 Tax=Phytophthora ramor... [more]
UPI001C093D0F1.300e-4241.57LOW QUALITY PROTEIN: secreted RxLR effector protei... [more]
A0A4Y9ZSL8_9AGAM1.440e-4246.75Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A2P4XPH2_9STRA1.510e-4243.43RxLR effector candidate protein (Fragment) n=1 Tax... [more]
A0A8K1FHK1_PYTOL1.650e-4246.15Uncharacterized protein n=3 Tax=Pythium oligandrum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig10contigH-paniculata_contig10:11460..12026 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeArthropoda
Seed ortholog score156.0
Seed ortholog evalue1.8e-35
Seed eggNOG ortholog7029.ACYPI24114-PA
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Reverse transcriptase (RNA-dependent DNA polymerase)
EggNOG OGs38DPC@33154,3BI7U@33208,3CTS1@33213,3SQRW@50557,41WYJ@6656,COG2801@1,KOG0017@2759
COG Functional cat.L
Best tax levelInsecta
Best eggNOG OGNA|NA|NA
Exons1
Model size567
Cds size567
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928706.8076997-CDS-H-paniculata_contig10:11459..120261622928706.8076997-CDS-H-paniculata_contig10:11459..12026Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10 11460..12026 +
1691480584.8572733-CDS-H-paniculata_contig10:11459..120261691480584.8572733-CDS-H-paniculata_contig10:11459..12026Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10 11460..12026 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig10.8.1prot_H-paniculata_contig10.8.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig10 11460..12026 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig10.8.1

>prot_H-paniculata_contig10.8.1 ID=prot_H-paniculata_contig10.8.1|Name=mRNA_H-paniculata_contig10.8.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=189bp
MEDPCREDFSACKRIFRYLSAVPDLGIMFDGKSNGHRLRGFVDADWAEDV
EDRKSTTGYVFKLNGGAINWSSRKQQFVAWSSTEAEYVAAGEAGNDTVYL
RQLLGSIGFGCMGSTTLFEDNRSAINLSNGSGAHCRPKHIGLRWHGIREW
IREGVLALKYVQIGDQLADILPKRLSRVKFQTLINLLM*
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mRNA from alignment at H-paniculata_contig10:11460..12026+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig10.8.1 ID=mRNA_H-paniculata_contig10.8.1|Name=mRNA_H-paniculata_contig10.8.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=567bp|location=Sequence derived from alignment at H-paniculata_contig10:11460..12026+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGAGGACCCATGTCGGGAAGACTTCAGTGCTTGCAAGCGCATTTTCCG CTATCTCAGTGCTGTGCCTGACCTCGGCATTATGTTCGACGGTAAGAGTA ATGGTCACCGTCTGAGAGGATTCGTTGATGCCGATTGGGCGGAAGATGTC GAAGACCGAAAGTCCACAACAGGATACGTATTCAAACTAAACGGTGGTGC GATAAATTGGTCCTCTAGGAAGCAACAGTTTGTCGCGTGGTCAAGTACAG AGGCAGAGTACGTTGCTGCAGGAGAAGCGGGAAATGATACGGTATATCTT AGGCAGTTACTAGGATCGATAGGTTTCGGATGCATGGGGAGCACGACATT GTTTGAGGACAATAGATCAGCTATCAATCTCAGTAATGGATCTGGCGCAC ATTGTAGGCCAAAGCACATCGGACTGCGCTGGCACGGAATTCGAGAGTGG ATTCGAGAAGGAGTATTGGCATTGAAATACGTGCAGATCGGCGATCAACT TGCTGACATTTTGCCGAAACGACTAAGCAGAGTAAAGTTTCAGACACTGA TAAATTTACTTATGTGA
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Coding sequence (CDS) from alignment at H-paniculata_contig10:11460..12026+

>mRNA_H-paniculata_contig10.8.1 ID=mRNA_H-paniculata_contig10.8.1|Name=mRNA_H-paniculata_contig10.8.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1134bp|location=Sequence derived from alignment at H-paniculata_contig10:11460..12026+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGAGGACCCATGTCGGGAAGACTTCAGTGCTTGCAAGCGCATTTTCCG
CTATCTCAGTGCTGTGCCTGACCTCGGCATTATGTTCGACGGTAAGAGTA
ATGGTCACCGTCTGAGAGGATTCGTTGATGCCGATTGGGCGGAAGATGTC
GAAGACCGAAAGTCCACAACAGGATACGTATTCAAACTAAACGGTGGTGC
GATAAATTGGTCCTCTAGGAAGCAACAGTTTGTCGCGTGGTCAAGTACAG
AGGCAGAGTACGTTGCTGCAGGAGAAGCGGGAAATGATACGGTATATCTT
AGGCAGTTACTAGGATCGATAGGTTTCGGATGCATGGGGAGCACGACATT
GTTTGAGGACAATAGATCAGCTATCAATCTCAGTAATGGATCTGGCGCAC
ATTGTAGGCCAAAGCACATCGGACTGCGCTGGCACGGAATTCGAGAGTGG
ATTCGAGAAGGAGTATTGGCATTGAAATACGTGCAGATCGGCGATCAACT
TGCTGACATTTTGCCGAAACGACTAAGCAGAGTAAAGTTTCAGACACTGA
TAAATTTACTTATGTGAATGGAGGACCCATGTCGGGAAGACTTCAGTGCT
TGCAAGCGCATTTTCCGCTATCTCAGTGCTGTGCCTGACCTCGGCATTAT
GTTCGACGGTAAGAGTAATGGTCACCGTCTGAGAGGATTCGTTGATGCCG
ATTGGGCGGAAGATGTCGAAGACCGAAAGTCCACAACAGGATACGTATTC
AAACTAAACGGTGGTGCGATAAATTGGTCCTCTAGGAAGCAACAGTTTGT
CGCGTGGTCAAGTACAGAGGCAGAGTACGTTGCTGCAGGAGAAGCGGGAA
ATGATACGGTATATCTTAGGCAGTTACTAGGATCGATAGGTTTCGGATGC
ATGGGGAGCACGACATTGTTTGAGGACAATAGATCAGCTATCAATCTCAG
TAATGGATCTGGCGCACATTGTAGGCCAAAGCACATCGGACTGCGCTGGC
ACGGAATTCGAGAGTGGATTCGAGAAGGAGTATTGGCATTGAAATACGTG
CAGATCGGCGATCAACTTGCTGACATTTTGCCGAAACGACTAAGCAGAGT
AAAGTTTCAGACACTGATAAATTTACTTATGTGA
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