mRNA_H-paniculata_contig267.7297.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 795 bits (2052), Expect = 5.620e-263
Identity = 465/1031 (45.10%), Postives = 617/1031 (59.84%), Query Frame = 2

Query:  545 LDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNN---NNAAPYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHD--------VAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILE--LVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKGDAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEGVEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKT----------------------------SKGVSNDD---------------------DLFFYFSEVF-------STSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDTIVEFDTYCPNFDCDGHGST-SASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGT-TGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPL 3424
            L  +Q+I  +SA  DFA    AY+AA  W   +L  +    + APY+ACADY QG  A  SLE  F   +VH VS++  +G CF+ TA+PS+A  +++ P  F L +A P L +LK+A  +LDH          A D D+ D   P          L++ +G  + LD V GLSV+LSPG +T    E      +R+WH+ L S S+++  +SFWSD +    E           R REW+RAA VV GL  +   +VGE+C F +  L+   DD + V G++HLLP     +E + AC+M LLS LA+KPEVLRV PL  AR  NAVA A+VQSAT T TPL DAGLDGTGEVIQVVDSG+DETSCFF DD GL++EHG  FDG+  VED +GNV     + +  +P+D +RRK+VQY++ FKT                            S+ +S DD                     DL ++FS +F       S+++ S TGGF  D   GHGT  AGSAAGA I          C   E+ GC G C+  +     + D+  + D +CP + CDG+    S+ CL DDP E L  H GVAPGA++++FDVS  G E    +AGN LW   + TGA +HTNSWG  +LC + E   LYDTFMYENPEHLL+F+AGN GGY+D P RE CT+ +PA+ KN L VGATSSG      TG DG L YE  G+ +Y P+G P +   P +G PS++ E  A +DTVA+FS  GPT D R+KP+VVAPGDQV +  S G+D  +C L    GTS +CP+VAG+AALVRQYF D +F+  D+V+RG C   +    C  F+PSAATVKA++INSA+LMGG + P  +RGFGRVHLEAGMP+ G G+  L V ++A+    G      T  V+  A +E+RATL+W+DPP++A +  QL HDLDL V++P G +Y MW    + + D  NV ERVIVP   +  G  W++ V++  L+T +Q YSLVV+G +
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAP----------LRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYD-------VTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVR-VED-DGNVS--TVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGA-ISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEP-AGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIAD--GEITELVT--VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMW---ESGVDDTVNVVERVIVPVESMESGV-WSVVVSAKALLTDEQLYSLVVTGAI 1013          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 749 bits (1934), Expect = 8.280e-246
Identity = 448/979 (45.76%), Postives = 588/979 (60.06%), Query Frame = 2

Query:  539 PSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNNNNA--APYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKG--------DAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEGVEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPA-TAAGYVSDTIV----EFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKT-EWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPLS 3427
            PSL+LV++ L T+A+ DF GH  A+ AA  W  ++L  + +   AP+L CA+YG G +    L+Q     +V  VSHS  +GACFLATA  + A  +++D  +FGL +  PF SALK+APG++DH         +S +   +   G   L  +HG T+ +  V GL+VKL+P T+  R       + +    LMS S+D+HS++FWSDP M  GE  H +   G +RAR+W++AA VVH L++    S G++CS+D   L  A DD + VTG+DHLL  G        +   E  +ACFM L+S  A +PEVLRV P H  R  N  AR I+QSA+ T+TPL+DAGLDGTGEVIQVVD+G+DETSCFF+DDGG +++HG FF+ +      +  + Y+     G + Y   RRKV+QY+E       V+ D D   + +             F  D + GHGT  AGSAAGA++    E   + C+  E  GCVG C+D + T    VS  I     + D  CP + CDG   ++  CL D+ +ETL  + G+A GAKL+IFDV    E F   E  GNG+W   +  G  VH+ S G D+LC   E  +LYD FMYENPE+LL+F+AGN G    +  R VCT+ +PAI KNVL VGA++SG +R   T                                     E  A ID VA FS YG T D R+KPEVVAPGD V +  S G+D  +C L    GTSM+CPIVAG+AA+VRQYF D  FYTAD+ ARG C  + GF C  FSPSAATVKA+LINSANLMG  + P   RGFGR+HLE GMPL+GEG LALFVAD+  TSIA  ++  Y F V+  A +E+RATLSWID P+++ + +QL++DLDLVVVSP G  YRMWG   + + D+ NVNERVIV A DV  G  WT+ V S  L+T  Q YSLVV+G +S
Sbjct:   32 PSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHS--------ESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGE--HLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIEL------VNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAE--TLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDG---STDPCLSDEVDETLTKNGGMAQGAKLSIFDVFF--EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDG----ETERSVCTMASPAIGKNVLAVGASTSGDTRVIST-------------------------------------EGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFC--SDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWG---SDVTDNRNVNERVIVDADDVETG-TWTVLVYSNSLVTDSQSYSLVVNGAIS 940          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 730 bits (1885), Expect = 6.570e-239
Identity = 437/986 (44.32%), Postives = 574/986 (58.22%), Query Frame = 2

Query:  539 PSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNNNNA--APYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKG--------DAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFD--GIEGVEDSNGNVDYHMAV-NDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDTIV---------EFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYT-GADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPLS 3427
            P+L+LV++ L T+A+ DF GH GA+ AA  W  ++L ++     AP+LACA YG G +A   L+Q     +V  VSHS  +GACF A+A  + A  +++D  +FGL    PF S LK+APG++DH  + D      +   E    G   L  +HG  +    V GLSV+L+PGT+  R  E    + +    LMS S+D+HS++FWSDP M +G   H + P G +RAR+W +AA VVH L++  +   G++CS++R  +  A DD + V+G+DHLL  G        +   E  +ACFM L+S LA +PEVLRV P    R  NA AR ++QSA+ T+TPL+DAGLDGTGE+IQV+DSG+DETSC+F+DD G +++HG +FD  G   V  S+ + D    + N G + Y   RRK                                             GHGT  AGSAAGA++    E   I C   +   CVG C+D   A    +D +V         + D  CP + CD   ST  +CL DD  ETL ++ G+A GAKLAIFDV   G +  +   GN LW V    G  +H+NS G D  C +    +L D FMYEN E+LL+F+AGN GG +    R  CTI TP I KN L +GA++SG +R   T GA G                                      IDTVA FS +G T D R+KPEVVAPGD V +  S G+D  +C L    GTSM+CPIVAG+AA++RQYF D  FYTAD+ ARG C  + GF C  FSPSAATVKA+LINSANLMGG + P   RGFGRVHLEAGMPL+GEG L LFVAD+  TSIAG ++  Y F V+  A +++RATLSWIDPP++A + +QL+H+LDLVVVSPDG  YRMWG  +    D+ NVNERVIV A DV  G  WT++V +  L+T  Q YSLVV+G +S
Sbjct:   27 PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHD------EAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGM-DGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFNGGDFSYYPERRKA--------------------------------------------GHGTHTAGSAAGATLNDPAE--TITCNVTDTLSCVGACIDLLDA----TDDLVSISSQTDGTDLDRLCPMYGCDN--STDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSS-IGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATG--------------------------------------IDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFC--SDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTT---DNRNVNERVIVDATDVVTG-TWTVWVWANNLVTDSQSYSLVVNGAIS 904          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 723 bits (1866), Expect = 5.430e-236
Identity = 436/1026 (42.50%), Postives = 588/1026 (57.31%), Query Frame = 2

Query:  545 LDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLN--NNNAAPYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKGDAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEGVEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKT-----------------------------------------SKGVSND------------DDLFFYFSEVF---------STSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDTIVEFDTYCPNFDCDGHGST-SASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGT-TGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPL 3424
            L  +Q+I  +SA  DFA    AY+AA  W  ++L +    + APY+ACADY QG  A  SLE  F   +VH VS++ T+G+CF+ TA+PS+A  ++  P  F L +A PFL +LK+A  +LDH        PD+                   H  A D        LS  +  +R                        S++  D   ++             R REW+RAA VV  L  +   + GE+C F + +L+   DD + V G++HLLP   A +EA+ AC+M LLS LA+KPEVLRV PL  A   NAVA A+VQSAT T TPL DAGLDGTGEVIQVVD+G+DETSCFF DD GL++EHG  F G+  VE  +GNV     + +  +P+D +RRK+VQY++ FKT                                         S   S+D            DDL ++FS +F         ++S+ SSTGGFD D+  GHGT  AG AAGA I      +   C   E+ GC G C+  +     + +   + D +CP ++CDG+    S+ CL DDP E L  H GVAPGA++++ D S  G +    +AGN LW   + TGA +H+NSWG D LC + E   LYD+FMYENPEHLL+F+AGN GG +D P  E CTI +PA+ KN+L VGATSSG SR   TG DG L YE +G+ +Y P+G P +   P +G PS++ E  A +DTVA+FS  GPT D R+KP+VVAPGDQV +  S G+D  +C L    GTS +CP+VAG+AALVRQYF D +F+  D+ +RG C   +    C  F+PSAATVKA++INSA+LMGG + P  LRGFGRVHLEAGMP+ G G+  L V ++ + S       +   +V+  A  E+RATL+W+DPP++A +  QL HDLDL V++P G +Y MW   ++   D  NV ERVIVP   +  G  W++ V++  L+T +Q YSLVV+G +
Sbjct:   25 LGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHG-------PDTSP-----------------HVGAAD-----DFNLSDLSAPLR------------------------STYGEDRSQYD-----------VTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVR-VE-GDGNVS--TVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGA-ISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEP-AGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGS---DDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGAD---DTVNVVERVIVPVESMESGV-WSVVVSAKGLLTDEQSYSLVVTGAI 973          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 698 bits (1802), Expect = 2.600e-225
Identity = 431/1029 (41.89%), Postives = 590/1029 (57.34%), Query Frame = 2

Query:  515 FPVPSSDYPSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNNNNA--APYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKGDA---------KAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFV-DDGGLKIEHGSFFD--GIEG----------VEDSNGNVDYHMAVND---------------GPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGG---------FDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDT------IVEFDTYCP---NFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGS-DEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPLS 3427
            +P  S  YPSL+LV++ L T+A+ DF GH  A+ AA  W  ++L +  +   AP+L CA+YG G +    L+Q     +V  VSHS   GACF A+A  + A  +++D  +FGL +  PF SALK+APG++DH    D+D        ++   G   L  +HG  + +  V GLS++L+PGT+  R  E    + +    LM  S+D+HS++FWSD  M  GE   F  P G +RAR+W++AA VVH L++    S G++CS++R ++  A+ D + V+G+DHLL  G            AE  +ACFM L+S LASKPEVLRV P       NA ARA +QSAT T+TPL+DAGLDGTG+V+QV+DSG+DE+SCFF  D+ G  + HG +F+  GI              D + ++ +  A +D               G +     RRK++QY+   K+     +  D F           T+S G          F+ D   GHGT VAGS AGA++    E  +I C D E   CVG C+D    A  V D       IV+ D  CP   ++DCD     +  CL DD  ETL ++ G+A GAKLAIFD   +     + + GNGLW         +H+NS+G D  C +    VLYD +M  NPE+LL+F+AGN G    +  R  CT+ +PAI+KNVL VGA++SG +R   T                           P +G           IDTVA FS YGPT D R+KPEV+APGD + +  S GS D  +C L    GTSM+ PIVAG++A++RQYF +  F+  D+ ARG C    G  C AFSPS+AT+KA++INSANLMGG + P   RGFGR+HLEAGMPL+GEG LALFV+DS  TSI  R+   Y F V  +A +++R TLSW+DPP++ F+ +QL+HDLDLVV+SP+G  Y MW   ++ + D  NVNERVIV AGDV  G  W++++ +  L T  Q YSLVV+G +S
Sbjct:   14 WPGKSLPYPSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKD--------KETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSF--PGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSF-----------TTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAE--LISCKDTETMSCVGACVDD-DEANDVDDLATPNVEIVDLDRLCPLFSDYDCDV--LETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG----ERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSAT---------------------------PGLG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG--CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMW---ASGVTDSANVNERVIVDAGDVETG-TWSVWIWADNLTTDVQSYSLVVNGAIS 967          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 683 bits (1762), Expect = 5.190e-222
Identity = 416/956 (43.51%), Postives = 544/956 (56.90%), Query Frame = 2

Query:  536 YPSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLN---NNNAAPYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPK------GDAKA-EAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEGVEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDTI----------VEFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGG 3343
            YPSL +V+  L T+A+  F GH  A+  A  W  + L +   +N  AP+L C +Y +G      L++     +V PV+HS+T+GACF  TA  + A  +   P  +GLT+  PF SALK+APGVL+H V+        DQ S+        L T HGH +    V GL+++LSPGT+    PE    +      LMS S+D+H  + WSDP    GE  H   P G +R REW+ AA +VH L      S G++CS+D  ++  A +D + V+G+DHLL K       D +A E ++ACFM L+S L+++ EVLRV P H  R  NAVAR I+QSAT T  PL+DAGLDGTG+VIQV+D+G+DETSCFF+DD G ++ HG +++ +                                                      E+  +S+++       D   GHGT  AGSA GA+I    E     C+  EV GCVG C+D A    +  D            ++ D  CP  DCD   +T   CL DD  +TL  H G+A GAKLA FDV  +G    T + GNGLW   +  G  +H+ S G D  C V E+ VLYD FMY NPE+L++ +AGNAG  +D   R VCT+ +PAI KN L VGATSSG +R   T ADG L            DG+                  YAD+DTVA FS YGPT+D R+KPEVVAPGD + +  S GSD  +C LS  +GTSMACPIVAG++A+VRQYF D +FY AD  ARG    +G   C +FSPS+ATVKA+LINSANLMGG + P   RGFGR+HLE GMPL GEG LALFVADS+ TS+       + F V+ +A ++ RATLSWIDPP+S+ + +QL+HDLDL VVSP G  + MW   ++   D  NVNERVI+ A DV  G
Sbjct:   31 YPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSP-YYGLTSISPFPSALKVAPGVLEHGVS-------LDQASQSPGR----LTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGE--HLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEAL-----------------------------------------------------GEIIPSSSSA------RDDVEGHGTHCAGSALGATIRTPAETPTT-CSGTEVPGCVGGCID-ADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDD--ATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSS-GTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDG--RTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLI-----------DGT----------------NGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGR-RCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWA--TSGEVDSRNVNERVIIAAEDVESG 876          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 644 bits (1662), Expect = 1.740e-206
Identity = 404/982 (41.14%), Postives = 538/982 (54.79%), Query Frame = 2

Query:  536 YPSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLN---NNNAAPYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVALDGVRGLSVKLSPGTVTMRHPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPK------GDAKA-EAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEGVEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDP---------ATAAGYVSDTIVEFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPL 3424
            YPSL +V+  L T+A + F GH   +  A  W    + +   +N  AP+L C +Y +G +    L++     +V  V+HS+T+GACF  TA  + A  +  +P  +GLT+  PF SALK+APGVL+H V+        DQ S+        L T HGH +    V GL+++LSPGT+    P+    +      LMS S+D+H  + WSDP    GE  H   P G +R REW+ AA +VH L      S G++CS+D  ++  A +D + V+G+DHLL K       D +A E ++ACFM L+S L+S+ EV RV P H  R  NAVAR I+QSAT T  PL+ AGLDGTG+VIQV+                + +       G      S GN  + ++ +                                                 GF  D  + HGT  AGSA GA+I    E     C+  EV GCVG C+D           T     S  + + D  CP   CD        CL D  E+TL  H G+A GAKL+ FD+  +G     ++ GNGLW   +  G  +H+ S+G D  C V +  +LYD FMY NPE+L++ +AGNAG  +D   R VCT+ +PAI KN L VGATSSG +R   T ADG              DG+                  Y+D+DTVA FS YGPT+D R+KPEVVAPGD V +  S GSD  +C LS  +GTSMACPIVAG++A+VRQYF D +FY AD  ARG C  +G   C +FSPS+ATVKA+LINSANLMGG + P   RGFGR+HLE GMPL GEG LALFVADS+ TS+   S   + F V+ +A ++ RATLSWIDPP+S+ + +QL+HDLDL VVSP G  + MW   ++   D  NVNERVI+ A +V  G  +T+ V++  L+T  Q YSLVV+G +
Sbjct:   31 YPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNP-YYGLTSISPFPSALKVAPGVLEHGVS-------RDQASQSPGR----LATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGE--HLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVIQY--------------INLIKADSVSGPASSTTSGGNRVWWVSPD-------------------------------------------------GFQGDDVDDHGTHTAGSALGATIRTPAETPTT-CSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPW--CLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDG--RTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRA-----------DGT----------------NGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGR-QCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWA--TSGEVDSRNVNERVIIAAENVESG-TYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 639 bits (1647), Expect = 1.830e-203
Identity = 408/993 (41.09%), Postives = 559/993 (56.29%), Query Frame = 2

Query:  530 SDYPSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNNNNAAPYLACADYGQGLDARDSLEQEFGFRSVHPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHV--LQTKHGHTVALDGVRGLSVKLSPGTVTMR-------HPEILELVRNWHSSLMSRSLDVHSSSFWSDPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNNSVGEVCSFDRANLQLADDDFMAVTGIDHLLPKG--------DAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIEG-----VEDSNGNVDYHMAVNDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGFDNDVANGHGTQVAGSAAGASIPIHGEDRVIPCADG-EVAGCVGKCLDPATAAGYVSDTIVEFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVA---GGGDWTIFVTSGELITPDQ-RYSLVVSGPLS 3427
            S  PSL   +  L T+A  DF     A+A A  WQ         ++PYL+C ++G+G  A   LE   G R+V PVS +AT+GACF+ TA P+ A  L    S    T  G F +ALKIAPG+LDH   G  D P + + +           L T HG ++ +D   GL V+LSPGT+          H  I EL++    SL S SLD+H+ + WSD  M  G+  H     G LR REW+RAA +V+  ++ +  +  +VC +++  +  A +D + V G+DHLL  G        +  AE +MAC+M L+S LAS+ EVL+V   +     NA ARA +QSA+  +TPL+DAGLDG+GEVIQ++D+G++ET+CF     G ++EHG +FD +          S     +      G + YD+ RRKV+QY++      G  +   + F  ++ F +         D+D+  GHGT  A + AG++I    E  ++ CADG EV GC G C+  ++     +D   + +  CP F C G       CL DD  +TL  H G+A GAK+A  DV   G+    + AGNGLW      G  +H+NS+G+D  C +    VLYD +MYEN EHLLLFSAGN G   D   R+ CTI++P I+KNVLTVG+TSSG +R   T  DGR                   GY        +  +  + ID V+ FSGYG T D R+KPEVVAPGD + +    G D  +C L    GTS +  + +G+AA+VRQYF DS+FY+AD+ +RG C    GF C +FSP A TVKA+LINSAN+MGG   P T RGFGR+HLEAGMPL G G +ALFVADS+   +       + F+V+P A +E+RATLSW DP +S+ +  QL++DLDL V +PDG  Y MW D     +D +NVNERVIVPA  VA      +W + V   +    D   +SLVV+G +S
Sbjct:   22 SQVPSLGFFEQTLLTTAGADFDSE-AAFAFASAWQAGD--RQGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQL---SSWEDFTTFGAFPAALKIAPGLLDH---GSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLK----SLSSPSLDLHAITCWSDLVMKGGD--HLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSSSGMPFVSADSFVS---------DDDL--GHGTHAATTVAGSTISSPAE--LVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD-----QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGR-------------------GY--------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQ--GFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDN---CWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAIS 944          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 606 bits (1563), Expect = 1.520e-189
Identity = 439/1101 (39.87%), Postives = 566/1101 (51.41%), Query Frame = 2

Query:  524 PSSDYPSLDLVQSILRTSARQDFAGHHGAYAAAQDWQRQQLLNNNNAAPYLACA--DYGQGLDARDSLEQEFGFRSV--HPVSHSATNGACFLATAVPSAAETLLQDPSRFGLTAAGPFLSALKIAPGVLDHDVAGDRDVPDSDQPSEKMFEGTHVLQTKHGHTVAL-------------------------------------DGVRGLSVKLSPGTVTMR----------HPEILELVRNWHSSLMSRSLDVHSSSFWS--------DPEMFEGENGHFARPEGKLRAREWTRAARVVHGLTQQDNN-SVGEVCSFDRANLQLADDDFMAVTGIDHLLPKGDAKA--------EAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVD-DGGLKIEHGSFFDGIEGVEDSNG---------NVDYHMAVNDGPYPYDTTRRK--VVQYMEYFKTSKGVSNDDDL-----------------FFYFSEVFSTSTTS-----STGGFDNDVANGHGTQVAGSAAGA--SIPIHGE--DRVIPCADGEVAGCVGKCLDPATAAG-------------YVSDTIVEFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYTGADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVA--------------GGGDWTIFVTSGELITPD-QRYSLVVSGPL 3424
            P+  +P L +++  L T+A   F  H G + AA +W   +L ++   APYLACA  D  +G     +L    G  ++    V  SA +G C +ATA P  AE++ Q    F   AA P  SALK+APG+LDH    DRD    D    +M  G  + + K                                             GV GL+V+L+PG +             +    EL   W   LMS+SLDVH  SFWS        D    EG         G++ AREWTRAA V+ GL   +   ++G+VCS+D   +   DDD + + G+DHLLP+G            E ++ACFM+LLS LAS+PEVLRV P    +  NA A   VQSA+ T +P++DAGLDGTGEVIQ+VD+G+D  SCFFVD DGG  + HG +F+ I GVE S+          NV        G + +D +RRK  VV        S  +S    L                    + E ++TS        S   F  D A GHGT  AGSAAGA  S P + +  D    C +G    CVG C+D     G             +     ++ D  CP  DCD        CLGDD  ETL  H GVA GA+LAIFD+  +      + AGNG+W   +  G+ + +NSWG+ + C      VL+D FMY N + LLLF+AGN G   DD +   CT+ TPA++KNVL VGA++SG  R  +T                + DG PT G                DID +AYFS +GPT D R+KPEVVAPGD + +    G    TC L    GTSM+ PIVAG+AALVRQYF +  FY  D+  RG C +   F C  F+PSAATVKA+LINSANLMGG   P  LRGFGRVHLEAGMPL GEG  ALFVADSAVTS A  ++  Y+F+V+ +A I++RATLSWIDPP++ F+ +QL+HDLDL V SP G  Y MW D +    D  N NERVIVPA ++                 G WT+ V S  L   + Q YSLVV+G +
Sbjct:   25 PTLPHPPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARL-DDARPAPYLACASPDSDRGGSGA-TLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFP--SALKLAPGLLDHP-HDDRDGGGGDGRL-RMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEI-GVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDD--LPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDG---DDWSG--CTMGTPALAKNVLAVGASTSGPGRLSFT----------------DEDGDPTDG-----------DSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDS---FGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGT---VDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077          

BLAST of mRNA_H-paniculata_contig267.7297.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 530 bits (1365), Expect = 4.400e-166
Identity = 338/733 (46.11%), Postives = 427/733 (58.25%), Query Frame = 2

Query: 1334 MAVTGIDHLLPKG--------DAKAEAQMACFMSLLSHLASKPEVLRVEPLHGARPANAVARAIVQSATETNTPLSDAGLDGTGEVIQVVDSGIDETSCFFVDDGGLKIEHGSFFDGIE--GVEDSNGNVDYHMAV-NDGPYPYDTTRRKVVQYMEYFKTSKGVSNDDDLFFYFSEVFSTSTTSSTGGF-------DNDVA-------NGHGTQVAGSAAGASIPIHGEDRVIPCADGEVAGCVGKCLDPATAAGYVSDTIV---------EFDTYCPNFDCDGHGSTSASCLGDDPEETLIHHTGVAPGAKLAIFDVSVNGEEFKTEWAGNGLWNVTVGTGAMVHTNSWGMDSLCLVDERSVLYDTFMYENPEHLLLFSAGNAGGYKDDPTREVCTIETPAISKNVLTVGATSSGASRTPYT-GADGRLRYEAYGIRNYEPDGSPTVGYYPMIGLPSSTPESYADIDTVAYFSGYGPTRDLRVKPEVVAPGDQVVTPDSGGSDEGTCALSGGTGTSMACPIVAGSAALVRQYFADSAFYTADLVARGLCGTTGGFPCGAFSPSAATVKAILINSANLMGGPTTPTTLRGFGRVHLEAGMPLQGEGKLALFVADSAVTSIAGRSMNSYTFSVEPSAAIEIRATLSWIDPPSSAFAELQLIHDLDLVVVSPDGEEYRMWGDPSNPLYDDTNVNERVIVPAGDVAGGGDWTIFVTSGELITPDQRYSLVVSGPLS 3427
            M  TG+DHLL  G        +   E  +ACFM L+S LA +PEVLRV P    R  NA AR IVQSA+ T+TPL+DAGLDGTGE+IQV+DSG+DETSC+F+DD G +++HG +FD +    V  S  + D    + N G + Y   RRK++QY++        S+           F TS  S  G F       ++D A       +    +V  SAAGA++    E   I C   +   CVG C+D       V+D +V         + D  CP + CD   ST  +CL DD  ETL ++ G+A GAKLAIFDV   G+   +   GN LW V    G  +H+NS G D  C +    +L D FMYEN EHLL+F+AGN GG +    R  CTI TP I KN L +GA++SG +R   T GA G                                      I+TVA FS +G T D R+KPEVVAPGD V +  S G+D  +C L    GTSM+CPIVAG+AA++RQYF D  FY AD+ ARG C     F C  FSPSAATVKA+LINSANLMGG + P   RGFGRVHLEAGMPL+GEG L LFVAD+  TSIA  +++ Y F V+  A +++RATLSWIDPPS+A + +QL+H+LDLVVVSPDG  YRMWG  +    D+ NVNERVIV A DV  G  WT++V +  LIT  Q YSLVV+G +S
Sbjct:    1 MHATGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVNPDSPYSS-----------FYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAE--TITCNVTDTLSCVGACIDLLD----VTDDLVSISHQTGGTDLDRLCPMYGCDN--STDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFY-GDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEE----RSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATG--------------------------------------INTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDV--FICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETT---DNRNVNERVIVDATDVVTG-TWTVWVWANSLITDSQSYSLVVNGAIS 665