mRNA_H-paniculata_contig2204.5831.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig2204.5831.1
Unique NamemRNA_H-paniculata_contig2204.5831.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A482SLQ7_9ARCH (GTP-binding protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SLQ7_9ARCH)

HSP 1 Score: 134 bits (337), Expect = 6.690e-35
Identity = 73/116 (62.93%), Postives = 83/116 (71.55%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD LV SNGIIS +LAGKLR+LDSTE+EQKRGITMHSSAI+LLY M  K                 G+   +    K++FLINL+D PGHIDFSSDVSTA RLCD AL+VVDVLE
Sbjct:   36 LSDSLVSSNGIISPRLAGKLRFLDSTEEEQKRGITMHSSAIALLYRMEEK-----------------GAEAAKEIGTKEDFLINLVDCPGHIDFSSDVSTAARLCDGALIVVDVLE 134
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A836CNE8_9STRA (P-loop containing nucleoside triphosphate hydrolase protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNE8_9STRA)

HSP 1 Score: 132 bits (332), Expect = 1.560e-34
Identity = 85/156 (54.49%), Postives = 91/156 (58.33%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAP------------------------GPSPDEKKNGPGGSAGEQ----------------AEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSDGLVCSNGIISSKLAGKLRYLDST DEQ+RGITM SSAISLLY +  K + A                         G + D +    G  A EQ                A  A   +LINLIDSPGHIDFSSDVSTATRLCD  LVVVDVLE
Sbjct:  113 LSDGLVCSNGIISSKLAGKLRYLDSTADEQQRGITMRSSAISLLYELQLKGKRAAAAHAXXXXXXXXXXXXXXAVQNGTGAAADSEAAAAGTPASEQTXXXXXXXXXXXXXXXATPAPGPYLINLIDSPGHIDFSSDVSTATRLCDTGLVVVDVLE 268
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A6A4ZWQ2_9STRA (Tr-type G domain-containing protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A4ZWQ2_9STRA)

HSP 1 Score: 123 bits (308), Expect = 3.240e-32
Identity = 68/116 (58.62%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD LV SNGIIS KLAGK+R+LD+TE+EQ RGITM SSAISL+Y  P         +P E+K G   S  +        +LINL+DSPGH+DFS DVSTA RLCD ALV++DV+E
Sbjct:   36 LSDSLVSSNGIISDKLAGKVRFLDNTEEEQTRGITMKSSAISLMYEGPL--------TPSERKAGGLDSTNDDVTVP---YLINLVDSPGHVDFSFDVSTAVRLCDGALVLIDVIE 140
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A428T4S2_9HYPO (Ribosome assembly protein 1 n=1 Tax=Fusarium sp. AF-4 TaxID=1325735 RepID=A0A428T4S2_9HYPO)

HSP 1 Score: 119 bits (299), Expect = 8.210e-32
Identity = 64/116 (55.17%), Postives = 83/116 (71.55%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            L+D L+ +NGIIS KLAGK+RYLDS  DEQ RGITM SSAISL ++M  +++ AP   P++K+                 +L+NLIDSPGHIDFSS+VSTA+RLCD A+V+VD +E
Sbjct:   35 LTDALLATNGIISPKLAGKIRYLDSRPDEQTRGITMESSAISLYFAM--RRKTAPDAEPEDKE-----------------YLVNLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVE 131
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: W7U9X5_9STRA (Elongation factor ef-2 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7U9X5_9STRA)

HSP 1 Score: 127 bits (320), Expect = 9.140e-32
Identity = 74/131 (56.49%), Postives = 91/131 (69.47%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRP----------APGPSPDEKKNGPGGS-----AGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD LVCSNGIIS KLAGKLR+LDS+E+EQKRGITM +SAISLL++  +++ P          AP  SP       GG+     + E       ++LINLIDSPGHIDFSSDVSTATRL D A+++VD+LE
Sbjct:   36 LSDSLVCSNGIISPKLAGKLRFLDSSEEEQKRGITMKASAISLLHTKKTRRFPIVDLSTPVTNAPRESPGAIAVSQGGTDVGSLSAELPYPVSQDYLINLIDSPGHIDFSSDVSTATRLSDGAIIIVDILE 166
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A7D8YN68_9HELO (Ribosome assembly protein 1 n=5 Tax=Lachnellula TaxID=47830 RepID=A0A7D8YN68_9HELO)

HSP 1 Score: 127 bits (319), Expect = 1.240e-31
Identity = 70/116 (60.34%), Postives = 84/116 (72.41%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD L+ +NGIIS KLAGK+RYLDS  DEQ RGITM SSAISL +SM   +R APG +P+EK+                 +LINLIDSPGHIDFSS+VSTA+RLCD A+V+VD +E
Sbjct:   35 LSDALIATNGIISPKLAGKIRYLDSRPDEQTRGITMESSAISLFFSM--LRRSAPGATPEEKE-----------------YLINLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVE 131
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A8H4V0V2_9HYPO (Tr-type G domain-containing protein n=1 Tax=Fusarium sp. NRRL 66182 TaxID=2692428 RepID=A0A8H4V0V2_9HYPO)

HSP 1 Score: 121 bits (304), Expect = 1.340e-31
Identity = 68/116 (58.62%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            L+D L+ +NGIIS KLAGK+RYLDS  DEQ RGITM SSAISL +SM   +R AP  +P+ K+                 +LINLIDSPGHIDFSS+VSTA+RLCD ALV+VD +E
Sbjct:    7 LTDSLIATNGIISPKLAGKIRYLDSRPDEQLRGITMESSAISLYFSM--LRRSAPDAAPETKE-----------------YLINLIDSPGHIDFSSEVSTASRLCDGALVLVDAVE 103
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A4D9CXS8_9STRA (Tr-type G domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CXS8_9STRA)

HSP 1 Score: 126 bits (317), Expect = 1.370e-31
Identity = 74/131 (56.49%), Postives = 90/131 (68.70%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRP----------APGPSPDEKKNGPGGS-----AGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD LVCSNGIIS KLAGKLR+LDS+E+EQKRGITM +SAISLL++   ++ P          AP  SP       GG+     + E       ++LINLIDSPGHIDFSSDVSTATRL D A+++VD+LE
Sbjct:   36 LSDSLVCSNGIISPKLAGKLRFLDSSEEEQKRGITMKASAISLLHTKKIRRFPIVDLSTPVTSAPRESPGAIAVSQGGTDVGSLSAELPYPVSQDYLINLIDSPGHIDFSSDVSTATRLSDGAIIIVDILE 166
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: A0A1V9ZMG1_9STRA (Elongation factor Tu GTP-binding domain-containing protein (Fragment) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZMG1_9STRA)

HSP 1 Score: 122 bits (307), Expect = 1.830e-31
Identity = 71/116 (61.21%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            LSD LV SNGIIS +LAGK+RYLD+TE+EQ RGITM SSAISLLY  P         +P EKK   G S  E        +LINL+DSPGH+DFS DVSTA RLCD ALV++DV+E
Sbjct:   33 LSDSLVSSNGIISDRLAGKIRYLDNTEEEQLRGITMKSSAISLLYEGPL--------TPQEKK---GRSDDETVP-----YLINLVDSPGHVDFSFDVSTAVRLCDGALVLIDVIE 132
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BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Match: B8NM15_ASPFN (GTP-binding protein lepa, putative n=1 Tax=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) TaxID=332952 RepID=B8NM15_ASPFN)

HSP 1 Score: 122 bits (307), Expect = 2.420e-31
Identity = 68/116 (58.62%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:    1 LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSKQRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVSTATRLCDCALVVVDVLE 348
            L+D L+ +NGIIS KLAGK+RYLDS  DEQ RGITM SSAISL +SM   +RPAP  +P  K+                 +LINLIDSPGHIDFSS+VSTA+RLCD A+V+VD +E
Sbjct:   35 LTDSLIATNGIISPKLAGKIRYLDSRPDEQLRGITMESSAISLFFSM--MRRPAPDAAPVAKE-----------------YLINLIDSPGHIDFSSEVSTASRLCDGAIVLVDAVE 131
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig2204.5831.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A482SLQ7_9ARCH6.690e-3562.93GTP-binding protein (Fragment) n=1 Tax=archaeon Ta... [more]
A0A836CNE8_9STRA1.560e-3454.49P-loop containing nucleoside triphosphate hydrolas... [more]
A0A6A4ZWQ2_9STRA3.240e-3258.62Tr-type G domain-containing protein n=1 Tax=Aphano... [more]
A0A428T4S2_9HYPO8.210e-3255.17Ribosome assembly protein 1 n=1 Tax=Fusarium sp. A... [more]
W7U9X5_9STRA9.140e-3256.49Elongation factor ef-2 n=1 Tax=Nannochloropsis gad... [more]
A0A7D8YN68_9HELO1.240e-3160.34Ribosome assembly protein 1 n=5 Tax=Lachnellula Ta... [more]
A0A8H4V0V2_9HYPO1.340e-3158.62Tr-type G domain-containing protein n=1 Tax=Fusari... [more]
A0A4D9CXS8_9STRA1.370e-3156.49Tr-type G domain-containing protein n=1 Tax=Nannoc... [more]
A0A1V9ZMG1_9STRA1.830e-3161.21Elongation factor Tu GTP-binding domain-containing... [more]
B8NM15_ASPFN2.420e-3158.62GTP-binding protein lepa, putative n=1 Tax=Aspergi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig2204contigH-paniculata_contig2204:12197..13015 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score163.3
Seed ortholog evalue7.1e-38
Seed eggNOG ortholog2880.D7G3I9
Preferred nameEFTUD1
KEGG koko:K03234,ko:K14536
KEGG Pathwayko03008,ko04152,ko04921,map03008,map04152,map04921
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0001882,GO:0001883,GO:0003674,GO:0003676,GO:0003723,GO:0003746,GO:0003824,GO:0003924,GO:0005488,GO:0005515,GO:0005525,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006139,GO:0006163,GO:0006412,GO:0006414,GO:0006417,GO:0006448,GO:0006518,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0006996,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009116,GO:0009117,GO:0009119,GO:0009141,GO:0009144,GO:0009150,GO:0009199,GO:0009205,GO:0009259,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010608,GO:0010628,GO:0016043,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0017076,GO:0017111,GO:0019001,GO:0019222,GO:0019538,GO:0019637,GO:0019693,GO:0022607,GO:0022613,GO:0022618,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0032268,GO:0032270,GO:0032549,GO:0032550,GO:0032553,GO:0032555,GO:0032561,GO:0034248,GO:0034250,GO:0034622,GO:0034641,GO:0034645,GO:0035639,GO:0036094,GO:0042254,GO:0042255,GO:0042256,GO:0042278,GO:0042802,GO:0043021,GO:0043022,GO:0043043,GO:0043167,GO:0043168,GO:0043170,GO:0043603,GO:0043604,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0044877,GO:0045727,GO:0045901,GO:0046039,GO:0046128,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051246,GO:0051247,GO:0055086,GO:0060255,GO:0065003,GO:0065007,GO:0070925,GO:0071704,GO:0071826,GO:0071840,GO:0072521,GO:0080090,GO:0097159,GO:0097367,GO:1901068,GO:1901135,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1901657,GO:1990145,GO:2000112
EggNOG free text desc.GTPase activity
EggNOG OGsCOG0480@1,KOG0467@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03009,ko03012,ko04147
Exons2
Model size366
Cds size366
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929605.0280743-CDS-H-paniculata_contig2204:12196..124851622929605.0280743-CDS-H-paniculata_contig2204:12196..12485Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2204 12197..12485 -
1691480932.5949755-CDS-H-paniculata_contig2204:12196..124851691480932.5949755-CDS-H-paniculata_contig2204:12196..12485Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2204 12197..12485 -
1622929605.0511644-CDS-H-paniculata_contig2204:12938..130151622929605.0511644-CDS-H-paniculata_contig2204:12938..13015Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2204 12939..13015 -
1691480932.6112742-CDS-H-paniculata_contig2204:12938..130151691480932.6112742-CDS-H-paniculata_contig2204:12938..13015Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig2204 12939..13015 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig2204.5831.1prot_H-paniculata_contig2204.5831.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig2204 12197..13015 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig2204.5831.1

>prot_H-paniculata_contig2204.5831.1 ID=prot_H-paniculata_contig2204.5831.1|Name=mRNA_H-paniculata_contig2204.5831.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=122bp
LSDGLVCSNGIISSKLAGKLRYLDSTEDEQKRGITMHSSAISLLYSMPSK
QRPAPGPSPDEKKNGPGGSAGEQAEAAKDNFLINLIDSPGHIDFSSDVST
ATRLCDCALVVVDVLEVSEPLS
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mRNA from alignment at H-paniculata_contig2204:12197..13015-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig2204.5831.1 ID=mRNA_H-paniculata_contig2204.5831.1|Name=mRNA_H-paniculata_contig2204.5831.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=819bp|location=Sequence derived from alignment at H-paniculata_contig2204:12197..13015- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTGTCCGACGGGCTCGTGTGCTCCAATGGTATAATCTCTTCCAAGTTGGC
TGGCAAGCTAAGATACCTGGACAGCACGTGAGATCGCGTAACGACGTAGG
TTTTACCACGTGCAAAACACTGGACGCTGCGTACGTTCTTTGCTACGACT
TCGGCCGTGTAATGCTATGCATATTGCATCTTTAGCGCAGTATCCCCGTC
CAACATGATGAGGATACTACTGCAGTATTCCGTATTCTACATTATGAGAC
GTGCATACCCGGCGCACCGGCTTGTCACCGATGGCGAATTATAACCGTCT
ACGTCTAGTCTATGGTCTCCACTTGTCCCACTCATAATGCTTTCGGTATT
GAGAATTAGTCGTCGACAGCGAAATTGGAGTCGAATGCTGGCTCGTCCTA
CTGGACCCGACTTTACTACAGCAGTCGTCCCGTTATTGTCGAACCATTCG
CGTTTTCAAAAATCGACCGTGCTTCTTGTCTTATCATGCTGGTCAACTGA
CCGGGCAATTCCATGGCTCTTTTTTTTCAGGGAAGATGAACAGAAGAGAG
GCATCACGATGCACTCCAGCGCGATATCGCTACTGTACTCCATGCCGTCG
AAGCAAAGGCCAGCTCCCGGACCCTCGCCCGACGAAAAAAAAAACGGTCC
AGGTGGGTCGGCGGGCGAGCAGGCGGAGGCGGCCAAGGACAATTTCCTCA
TCAACCTGATTGATTCTCCGGGTCACATTGACTTTAGCTCGGACGTATCC
ACGGCGACCCGACTGTGTGACTGTGCGCTAGTGGTGGTAGACGTGTTGGA
GGTGAGCGAACCTCTGTCC
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Coding sequence (CDS) from alignment at H-paniculata_contig2204:12197..13015-

>mRNA_H-paniculata_contig2204.5831.1 ID=mRNA_H-paniculata_contig2204.5831.1|Name=mRNA_H-paniculata_contig2204.5831.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=732bp|location=Sequence derived from alignment at H-paniculata_contig2204:12197..13015- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTGTCCGACGGGCTCGTGTGCTCCAATGGTATAATCTCTTCCAAGTTGGC
TGGCAAGCTAAGATACCTGGACAGCACTTGTCCGACGGGCTCGTGTGCTC
CAATGGTATAATCTCTTCCAAGTTGGCTGGCAAGCTAAGATACCTGGACA
GCACGGAAGATGAACAGAAGAGAGGCATCACGATGCACTCCAGCGCGATA
TCGCTACTGTACTCCATGCCGTCGAAGCAAAGGCCAGCTCCCGGACCCTC
GCCCGACGAAAAAAAAAACGGTCCAGGTGGGTCGGCGGGCGAGCAGGCGG
AGGCGGCCAAGGACAATTTCCTCATCAACCTGATTGATTCTCCGGGTCAC
ATTGACTTTAGCTCGGACGTATCCACGGCGACCCGACTGTGTGACTGTGC
GCTAGTGGTGGTAGACGTGTTGGAGGTGAGCGAACCTCTGTCCGGAAGAT
GAACAGAAGAGAGGCATCACGATGCACTCCAGCGCGATATCGCTACTGTA
CTCCATGCCGTCGAAGCAAAGGCCAGCTCCCGGACCCTCGCCCGACGAAA
AAAAAAACGGTCCAGGTGGGTCGGCGGGCGAGCAGGCGGAGGCGGCCAAG
GACAATTTCCTCATCAACCTGATTGATTCTCCGGGTCACATTGACTTTAG
CTCGGACGTATCCACGGCGACCCGACTGTGTGACTGTGCGCTAGTGGTGG
TAGACGTGTTGGAGGTGAGCGAACCTCTGTCC
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