mRNA_H-paniculata_contig2162.5705.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: D7G4Q2_ECTSI (Vacuolar protein 8 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G4Q2_ECTSI)

HSP 1 Score: 1913 bits (4955), Expect = 0.000e+0
Identity = 1010/1370 (73.72%), Postives = 1161/1370 (84.74%), Query Frame = 1

Query:    1 MVIGPQSRCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVAS------RTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGG---SRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKCNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKLGPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHSCTVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAMSAVGLSNLAADPGNHWRVLESENMLNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRNRDTHASAIGLILFNISCNPKLAGSLLDDGLTVPMLVDLMKKHGPSVQAACLGVMQNVSTNNAFHRRLLEQGVLEALDAAKDVDGGALSAQCAAVLYNFSLFEQSLTHMVDLGAIFLATHLSYSNVIKTKQLCVATFCNMTIHKVIVEESFLSSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSV-KGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQENTSSRAAFGLLAGTMRAGSLYLTTRLDALRTMVNLVTHYLPARQTAVETHVTSALCIILKALIDEEEKLLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVENAEIKQDVATALCRLSASLELAFDIVDEGLAEALFWLTLEDLLGLTKSVFLRCSVVCRNVALCDNALKRISSESARIFKVMERLISSGDPELLPNIAIVCLRITGTPESMLAFHREGLVAQMVYLSEKGHEEVKQICSAALNQVPPDMVELDDKMVKVLVSLLQGAESWSIGDVGHAVPEPSLHDLKSWTLRQALVTENPVDVQPSWINDVCLDLE 4080
            MVIGPQ RCA LAQERY +FPKEGR E LRP+L +LGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVV ALN HIKHMK+VH ++E+KKAERREADRKR VQ+A GEG    ++AS        E  S+T LY++GVALPRL RP  A L  DSIE+GIIYMSRW QDN K D + S+  E  A    LE  + +P+  E  D  DV G   S+D+ N  K+QAQ+KC LAL+NMTMRDQMS+AFLDEYGLLPPLLEI + NQ VDVL +GLAC++NIL+E Y+I+KLVE GL+ V RPLSGHEDE+VQQ+ A I LAIS+C GLEEWLVQDGAIPALN LARS+ +LTA+LA GGLVNIAITLTAAQADSMQRVV+RT + LL   C+ D LHFCALA KNLTVLDNVRAYLDDQVA IA+DILA+LGPDS DTVILC+AAIFNC+  KQSR+RATDK++V EC+RL+S+C +DAQHSCTVLLAELSK+ DV N LLDGGI+ +FS NLS+ DPRSVA+SA GLS+LAADP NHWRVLES NML MLL+A+VLDH +AQ H+L LLCGL SNE+TQA VV+AGVV AVQ M NRD HASAI LILFNISCNP L+GSLLD+ L VPMLV+L+KKH  SVQAACLG ++N+S+  AFHR+LLE+GVLEA+D++KDVDGGALSAQCAA+LYNFS  E+S++ M++LG IFL THLSYSN+IKTKQLC A FCN+TIHKVI +ESFL++L+ LSTSTEA LV+C AKAL+NLSTYPRGRS LG+NK+VVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFL K+++++LV++G+IQDLIAVTVLR+EEVKTKETLA AIFNLLAREDTR L+ADQDAVFALVRLTRLQSPDLN ICVRAIYNLTCEM  Y+ KLLEMEAERVLV QASFPNGGV VKKMCGAAL MMSS  K ASC+LAK+G+VGA RAIMCV+D DTLEH+AT AFNLSREDSC   +AAQD+T+VL +LHE G TIVK +CVAT+CN SSS  AQ+N +S AAFG+LA T+RAGSL L TRLDALRT+VNLVTHY P R+ AVE+  TSALC+ILKAL+DEE+KLLVSKALRDM+ + QGH QMM + VLPALVRLAKVENAEIKQDVATALCRLSAS+ELAFD+VDEGL EAL+WLTLEDLLGL KSV LRCSVVC NV L D+AL+R+S ESAR  KV++RL  + D ELL N+A+VCLRITG  ESMLAFH++GLVA M+ LS +G E+VKQICS ALNQVPPDMV+LDDKMVKVLVSLL  + S  IGD GH V EPS+HDLK W+LR A + ENPVDVQ SW+N VC D E
Sbjct:    1 MVIGPQPRCAILAQERYVKFPKEGRVEFLRPYLARLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVAALNHHIKHMKLVHDHIENKKAERREADRKRQVQNAIGEGVSSSKIASCMKRRPPLETSSDTCLYNEGVALPRLDRPADASLRPDSIESGIIYMSRWRQDNGKGDKLASDKGEKDALHEKLERMSVSPQHDEAEDDGDVDGLQASKDDMN--KMQAQRKCCLALVNMTMRDQMSQAFLDEYGLLPPLLEITHANQTVDVLLMGLACILNILSEEYKINKLVEAGLIGVARPLSGHEDERVQQHAAGIFLAISSCSGLEEWLVQDGAIPALNALARSATVLTAQLATGGLVNIAITLTAAQADSMQRVVMRTVTNLLSGSCDSDGLHFCALAAKNLTVLDNVRAYLDDQVAGIAIDILARLGPDSDDTVILCTAAIFNCVAQKQSRLRATDKNLVAECQRLISVCGSDAQHSCTVLLAELSKHTDVANRLLDGGILDIFSTNLSAADPRSVAISAAGLSHLAADPDNHWRVLESGNMLTMLLQALVLDHALAQRHVLRLLCGLASNEATQAEVVSAGVVRAVQEMSNRDMHASAISLILFNISCNPSLSGSLLDESLAVPMLVELVKKHNLSVQAACLGALKNLSSVTAFHRQLLERGVLEAVDSSKDVDGGALSAQCAAILYNFSFEEKSISKMMELGGIFLVTHLSYSNIIKTKQLCAAAFCNVTIHKVITDESFLAALLLLSTSTEAVLVLCSAKALSNLSTYPRGRSSLGSNKNVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLQKESIVTLVRDGMIQDLIAVTVLRVEEVKTKETLARAIFNLLAREDTRSLVADQDAVFALVRLTRLQSPDLNTICVRAIYNLTCEMSRYERKLLEMEAERVLVVQASFPNGGVHVKKMCGAALTMMSSSGKVASCSLAKKGIVGALRAIMCVRDKDTLEHVATTAFNLSREDSCLPTMAAQDITTVLVSLHEFGNTIVKNLCVATMCNFSSSLEAQDNLASPAAFGVLANTVRAGSLSLATRLDALRTVVNLVTHYAPGREKAVESSTTSALCVILKALVDEEDKLLVSKALRDMSSYAQGHPQMMKEDVLPALVRLAKVENAEIKQDVATALCRLSASVELAFDMVDEGLPEALYWLTLEDLLGLNKSVLLRCSVVCCNVVLSDDALRRVSGESARFSKVLQRLSDTSDSELLLNVAMVCLRITGLRESMLAFHKDGLVAHMLDLSGRGDEDVKQICSTALNQVPPDMVQLDDKMVKVLVSLLTASGSSIIGDSGHNVSEPSVHDLKPWSLRSASIAENPVDVQSSWVNYVCQDFE 1368          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A7S4E603_9STRA (Vacuolar protein 8 n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4E603_9STRA)

HSP 1 Score: 461 bits (1185), Expect = 1.760e-132
Identity = 421/1406 (29.94%), Postives = 699/1406 (49.72%), Query Frame = 1

Query:    7 IGPQSRCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPR-RNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGII-YMSR-WAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEG--YRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSD-ILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKC-----NGDSLHFCALAVKNLTVLDNVRAYLDDQ--VAAIAVDILAKLGPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHSCTVLLAELSKYRDVTNNLLDGGIMQLFSDNL-SSFDPRSVAMSAVGLSNLAADPGNHWRVLESENMLNMLLRAVVLDHTV---AQHHILCLLCGLVSNESTQANVVAAGV----VGAVQAMRNRDTHASAIGLILFNISCNPKLAGSLLDDGLTVPMLVDL----MKKHGPSVQA--------------ACLGVMQNVSTNNAFHRRLLEQGVLEALDA----------AKDVDGGALSAQCA--AVLYNFSLFEQSLT-------HMVDLGAIFLATHLSYSNVIKTKQLCVATFCNMTIHKVIVEESFLSSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGN-NKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSV-----KGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTI---VKKICVATLCNLSSSSVAQENTSSRAAFGLLAGTMRAGSLYL-TTRLDALRTMVNLVTHYLPARQTAVETHVTSALCIILKALIDEEEKLLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVENAEIKQDVATALCRLSASLELAFDIVDEGLAEALFWLTLEDLLGLTKSVFLRCSVVCRNVALCDNALKRISSESARIFKVMERLISSGDPELLP-NIAIVCLRITGTPESMLAFHREGLVAQMVYLSEKGHEEVKQICSAALNQVPPDMVE-LDDKMVKVLVSLLQGAESWSIGDVGHAVPEPSLHDLKSWTLRQA 4014
            IG   +C   A+E    FP E R   LRPF+ +  LFP   ++  +API ++EL+ LVR W LH+++ FW ++ T++ +V AL   +++      N    + +RR AD  R  + A      PQ +                         +  E   D  +T ++ YMSR + +   + +  +   T+    K    D+  + E  E+             + ++++ ++KCS ALLNM++  +MS  F+++ G+   LL++ +  +  +++    A + N++  G  Y   KL + G+V V+  L   +D +V+ ++A  L  +S    LE+ L   GA+ A   L   SD + T ++AA  L+N+A ++   QAD+  + V++  + L+  +      + ++  FCA A+  +  L   R  L  Q  VA + V  LA   P +++    C++A+ N       R    +  +VK   RLM       Q  CT+ L  L+   D+  +LL  G ++  ++ + +  D   V   A  L   A DP     V+  E  L  L+  +  D  V    Q + L  LC L+++ +T   V+ AGV    VG    +    +    + + + N+S +  +   +      + ++++L    + +  PS +               + L  + N+S ++  H  LLE+     LDA          A+  D    S  C   + L+  SL    LT        ++   A  L   L+ ++  +T+     +  NMT    + EE+ + +L+ LS S+E   V+ CA   ANLSTYP+GR+ LG  + S+VPAL+ MMRSGV DA +VQ+  A+A+CN LSVFL K +V+ +V  G +QD+I +TVLR  +V+TK+ LA A+FNLLAR DTR+ + + D   ALVRLTR++ P LN +    + NL+CE      KLLEM   RVLV+Q    +GGV +K+ C A LA +++V     KG  C   +  +V   R+I   +D +TLE++ATI + LS     R  L AQ+   VLA+L   G+ +   V+++ VA L ++S+ S   E+ +   A  L+  TM AG+++   TR++A+  + NLV H+ P+R  AV      AL   +++   +E   +V+K LRD+T   +    ++  G +    RLAK E A +K DVA  +C L AS      +++E    ALFWLTL+DLL LT++V + C+   R +A     +  I  E A +  ++ R     + E +  + A+V     G   +  A  R G +  +  L+  G E ++++CSAAL+Q+P ++++ +D K++ VL+ LL   ++    D    +P+ SL   K W LR+A
Sbjct:   26 IGHVPKCTQKAREFLKGFPAEERAAALRPFMIRYRLFPDNCKDVHTAPITIKELKGLVRVWKLHNQKGFWSDHTTREQIVLALYERMQY------NYRQVREKRRRADEDRKRREALKHQDGPQ-LTEXXXXXXXXXXXXXXXXXXXXXXXLEEEPKKDQDQTPLLMYMSRGFGEPEDRCNPRSPSPTKK--KKKFFSDERPSFEESEDXX----------XDMQQVRIKRKCSTALLNMSLNKKMSSQFVEQGGM-AALLDLASTCKDEEIITNCAAALNNLIPYGDYYPPWKLCDLGVVPVIVKLVKSDDARVRHFMALCLCRLSQEHQLEDRLAGQGALGAATRLVAVSDSVRTKEIAAKVLINLACSMEGHQADTTVKNVLKCVAVLVTHRDKNNNPDAETQQFCAEAILVMACLPQARPVLAKQGVVALLKVMFLASQRPATTNA---CASALCNMGQAHSCRKEILNLGLVKIMARLMKTGEEATQRICTLCLTALAAQADLRPSLLKEGALRTIAEVVYARKDADLVKQGAGALLAFAFDPSTREDVVH-EGCLGALVALLDKDDKVDEETQANSLMALCNLIADAATCPQVLEAGVLLKLVGYTSQLTELPSLVDYLAVAVLNVSTHKDVRTYVARTPGCLDLIIELALLGVARKDPSGELIEGDGSGADSDRTKSALKTLLNLSLDSETHEALLERRRRSLLDALALLVYEDRKARTFDS---SRPCGKDSTLHLISLLLHILTTNKTNHDQLMSGDASKLLVCLAKTSNDETRTAVAGSLYNMTQLNPVAEENAIEALVRLSKSSENERVLWCAWCFANLSTYPKGRAMLGKLSASLVPALLGMMRSGVADAEKVQYHCAVAVCNTLSVFLKKQHVLDMVASGTVQDVIVITVLRANDVRTKQVLAQALFNLLARVDTRREMIECDVPMALVRLTRVEDPILNLLATNMLKNLSCEADKNVEKLLEMRVVRVLVSQCLSSSGGVQIKRKCAATLANLAAVPEILDKG-FCDR-QSNIVSGVRSIAVARDAETLEYVATICYYLSAMKKGRDELVAQEAVPVLASLCS-GEDVPAKVRQLVVAALTHISNDSTTHESLT-EFALPLIIETM-AGAVHAHDTRMNAMTLLCNLVVHHEPSRGAAVALEALPALKAFVRSCSVDEHFAVVAKILRDLTWDEEHVPLLIEQGAMALAARLAKREPAPLKHDVAAIVCNLCASGARPSQLIEEDAVGALFWLTLQDLLNLTRAVTVECATSLRYLAQHSEIVPLICDE-ANLLPLLLRFFKYDESEQVRYDAAVVLYYCLGHEPAQKALCRAGAIKMLSDLASTG-ERIREVCSAALHQLPNNLMQNVDGKLLGVLMGLLDMQDA-DFTDPATFMPDRSLTSRKPWPLREA 1396          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A835YYM6_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YYM6_9STRA)

HSP 1 Score: 329 bits (843), Expect = 5.870e-90
Identity = 262/798 (32.83%), Postives = 374/798 (46.87%), Query Frame = 1

Query: 2209 MTIHKVIVEESFLSSLIFLS-TSTEASLVMCCAKALANLSTYPRGRSFLGNNKSV-------------------------------------------------------------VPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVK----EGL-IQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDA----------------------VFALVRLTRLQSPDLN-----------------AICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQEN-----------------------------------------------TSSRAAFGLLAG----------------TMRAGSLYLTTRLDALRTMVNLVTHYLPARQTAVETHVTSALCIILKALIDEEEKLLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVENAE-----------------IKQDVATALCRLSA-----SLELAFDIVDEGLAE---ALFWLTLEDLLGLTKSVFLRCSVVCRNVALC------------------------DNALKRISSESARIFKVMERLIS-------------SGDPELLPNIAIVCLRITGTPESMLAFHREGLVAQMVYLSEKGHEEVKQICSAALNQVPPDMVELDDKMVKVL 3909
            M + +V+ E+SFL +LI L+ TS +++ ++CCAK +ANLS   RGR+F+G + +                                                              VP L++MMRSGVK+AA+VQ+  A+ALCNVLSV   KD +         EGL +QDLIAVTVLR+ EV TKE L+ A+FNLL R +TR+ + DQ                        VFAL++L RLQS + N                  +C+RAIYNLTCE+P YQ ++ E +  +V++ QASFPNGG +V+++CGAALA +S+       L K  VV A RA       DTLEH A   FN+SR    R ALA Q    V+ AL+E G  +VK +CVATL N+S +    +N                                               T      G+L G                T+ A  + L  RLDAL TM N+VT ++P+R  A      +ALC +LKAL  + +K+ +SK  R++         ++ +G++ AL +LAK E AE                 +KQDVA+ALCRLS      + E     + E  AE   A+FWLTLEDLLG T+SV LR ++ CRN+A                          +  +  + ++S R  +V+ +L               +   E   ++A+V L +T +   +    + GL+A M  L E   E V+Q+C+ ALNQ+P +MV+LD+K++K L
Sbjct:    1 MLLSRVVAEDSFLEALIALANTSADSARLLCCAKVMANLSGGSRGRAFMGLSTAAXXXXXXXXXXXXXXXXSIAVAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPCLVSMMRSGVKEAARVQYYCAVALCNVLSVAKLKDALAGATNMKTGEGLWLQDLIAVTVLRVNEVSTKEVLSRALFNLLTRAETRRKVVDQGTXXXXXXXXXTVVDQGKGVDQGTVFALIQLMRLQSSETNMXXXXXXXXXXXXXXXXTVCMRAIYNLTCELPEYQAEVEERDFYKVIMEQASFPNGGTEVRRLCGAALANLSAHPATCQVLPKHPVVSAVRAAAGTGLGDTLEHCAICLFNISRLPVGRIALALQGAGGVVPALNETGAVVVKTLCVATLANVSCTGNTDDNRNCYVDPAVVTGVKPRASTPASSXXXXXXXXXXXXXXXXSARGQRPATGDAPGPGMLGGALDCLCTAEVMAVLCATLSAAHMSLPCRLDALHTMCNMVTRHVPSRYAAASAGCCTALCTMLKALSSDAQKVPLSKCFRELVCEPACCRALLQEGLVTALSKLAKCEMAEXXXXXXXXXXXXXXXXEVKQDVASALCRLSTQEAMLTQEALLTALAEHAAEVVEAMFWLTLEDLLGATRSVLLRMAIACRNMAAXXXXXXXXXXXXXXXXXXXXXXVTEERCVTALCAQSDRFHRVLAKLXXXXXXXXXXXXXXXAEHAETRLHVAMVFLTLTASRAGIPLIAKGGLIASMSRLVEGSDERVRQVCATALNQLPQEMVQLDEKLIKSL 798          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A1V9ZA19_9STRA (Vacuolar protein 8 n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZA19_9STRA)

HSP 1 Score: 187 bits (474), Expect = 8.090e-44
Identity = 293/1346 (21.77%), Postives = 558/1346 (41.46%), Query Frame = 1

Query:   19 SRCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASR-TEGPSNTFLYHQGVALPRLARPVSAELPVD-----------SIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKCNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKLGPDSSDTVI--LCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHSCTVLLAELSKYRDVTNNLLDGGI-MQLFSDNLSSFDPRSVAMSAVGLS--NLAADPGNHWRVLESENMLNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAG----VVGAVQAMRNRDTHASAIGLILFNISCNPKLAGSLLDDGLTVPMLVDLMKKHGPS-------------VQAACLGVMQNVSTNNAFHRRLLEQGVLEALDAA--KDVDGGALSAQCAAVLYNFSLFEQSLTHMVDLGAIFLATHLSYSNVIKTKQLCVAT---FCNMTIHKVI-VEESFLSSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQENTSSRAAFGLLAGTMRAGSLYLTTRLDALRTMVNLVTHYLPARQTAV--ETH-VTSALCIILKALIDEEEKLLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVENAE-IKQDVATALCRLSASLELAFDIVDEGLAEALFWLTLEDLLG-----LTKSVFLRCSVVCRNVALCDNALKRISSESARIFKVMERLISSGDPELLPNIAIVCLRITGTPESML--AFHREGLVAQMVYLSEKGHEEVKQICSAALNQVPPDMVELDDKMVK 3903
            S+ A   QE      ++   +++R +LT+L LFP +R+P +APIR EELR LV+ W LH +RNFW+ + TK+D+V  L +HI + KV+  N ++    ++E                P R  S  T+ P+          + RL  PV     +D              +G+IY+SR A+       +     +NL       D+N       E +  D   ++ E   +++ A+  C+ +L  +TM      + + E G +P ++++ N + + +V +   A +VN+  +    +++++                                           ++PAL  +  ++D  T +     LVNIA    +    ++          +  +K       F    + NL++L   R    D      +D +  +    +D  +  + + A+ N      +       +++     L+ +     +   T   A LS  RD    ++D  +  +L +   + + PR      V L+  NLA     +  VL  + ++ +LL+      T+ +++ +  LC L+ +  ++A ++A      V+G   +   +     AIGL  FN SC    A   L   +  PM + L    GP+             VQ  CL  + N+S        L++   + A  A   K       + + A  + N +        M+    + +   LS +    TK+L +A+    CN+ +  V+   ++ L  LI LS + +A + + C+ A + L+     R  L     + P L+ MMRSG+++   +Q   A ALC +      + N   + KEG + D I  ++LRI    TKE  A  +FN+L  +D R  +     ++AL++L RL+S ++ A+C+ A+YNL+C+ P   P L+E     V+           + ++   A L  M+   G    L + GV+ A   +    D D + + A++  ++S + +    LA+     VL  +          + V  LCN+S  +   +          +   +   +      L   + + N+  H   A+  AV  + H + + + +     I +    ++++ +  ++   Q    M+ +G +  L+R+A V+ +         +L RLS        I+D+GL + L        LG     L   +  RCS++ R ++     + ++  + AR+  +++ L + GD E   N  ++   IT   +          G++  ++ L++ G  E  +IC+ AL  +  ++ E D + ++
Sbjct:    6 SQAARHTQELLEDLERDKELDIIRVYLTRLNLFPRQRDPRTAPIRSEELRDLVKHWKLHRQRNFWKNHTTKEDLVRTLYKHI-NTKVLP-NEKNPLPLQQEPPMTXXXXXXXXXAPSPTRPYSAGTDSPAKR-------RVSRLVSPVKVNEALDPYGGDLFGQRGDYHSGMIYVSRLAKPEKP--SVVETDLQNLL------DQNKPATLTPELEILDEDAAQRE---RRLMAE--CACSLYQLTMEPGHETSIVLE-GCIPAIVQLCNYDDL-EVKKYCSATIVNVSVDHALCARMIDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVPALISMLNNTDFETKEACVKTLVNIA----SFSGGNVSESXXXXXXXIAAKKDPAYD-RFIIETICNLSLLTGSRLKAPDDGI---LDPIPGINQTCNDLNVQTMIAMALSNFSGIDTNHHLMATPALLGALDGLLKLDDDTIKEMATTAAANLSTSRDAIPVIMDSALPTRLIA---TGYTPRQSIQENVTLAIANLALSDEANGLVLVEKGLVPLLLQLFAESSTLTRYNAMVALCTLMQHAGSRAELIAQDSIHVVIGLAASAELKLRELCAIGL--FNFSCYEDFAELTLQPDVLEPM-IRLFTSSGPAIGPKEEKEIVLSAVQEHCLNFLYNLSFYPQSRGTLVQATTVAACCAVFRKVAKTPEFNTRAAVTIANLTFCADGHPQMLAEDVLRVLRRLSAATS-ATKELLLASATALCNLAVPGVLHAGQTLLDLLIDLSHTPQADIALACSLAYSKLAADVALREVLSKCVELPPTLVVMMRSGIEE---IQIHCAAALCGMACERGPRSNK-HMWKEGTVSDFIVNSLLRINSDSTKEVCAKVLFNVLTHDDCRLAMIKDGVLYALIKLARLESLEIRALCITALYNLSCD-PSMVPALMEANVPHVVSKMCDGEFRHEESRRKLSACLTNMALAPGMELKLMEGGVLNAVLVLCEHADLDCMRYGASVLCSVSMQPANCDGLASNAALEVLRRMVHSKDPPQVILAVNALCNVSCHAHLHDKIEEAETICAILHVVSDANEDEGIILTCAKVLCNMAFH---AKHWAVMMKFHLIRTFIRVFANRPIYKSVADVLARIVATLSDAHQLVEAMVGEGAVQ-LLRMAAVDASPGTVVHCIVSLTRLSRGGHSGTRILDDGLFDIL---AAAVPLGDGTPRLAPDISERCSMILRTLSTYTMCIPQMIQD-ARLMPLIQALAAGGDKESCKNCVMLLHNITAARDRTFQKGVRISGVIPLLIRLAKVGSSEDGRICAVALAHINCELSEADRREIE 1299          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: W4GUR8_9STRA (Vacuolar protein 8 n=10 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GUR8_9STRA)

HSP 1 Score: 165 bits (417), Expect = 2.980e-37
Identity = 311/1428 (21.78%), Postives = 545/1428 (38.17%), Query Frame = 1

Query:   19 SRCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQ----AQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNI----LTEGYRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGA-----------------------------------------IPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKCNGDSLHFCALAVKNLTVLDNVRAYL-DDQVAAIAVDILAKLGPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSI---------CSADAQHSCTV-----LLAELSKYRDVTNNLLDGGI--MQLFSDNLSSFDPRSVAMSAVGLSNLAADPGNHWRVLESENMLNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRNRDTHASAIGLILFNISCNPKLAGSLLDDGLTVPMLVDLMKKHGPSV--------------QAACLGVMQNVSTNNAFHRRLLEQGVLEALDAA--KDVDGGALSAQCAAVLYNFSLFEQS--LTHMVDLGAIFLATHLSYSNVIKTKQLCVAT-FCNMTIHKVIVEESFL-SSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQENTSSRAAFGLLAGTMRAGSLYLTTRLDALRTMVNLVTHYLPARQTAVETHVTSALCIILKALIDEEEK---LLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVENAEIKQDV-ATALCRLSASLELAFDIVDEGLAEALFWLTLE------------DLLGLTKSVFLRCSVVCRNV-------------------------ALCDNALKRISS---------ESARIFKVMERLISSGDPELLPNIAIVCLRITG--TPESMLAFHREGLVAQMVYLSEKGHEEVKQICSAALNQVPPDMVELD 3888
            S+ A   QE      ++   +V+R FL +  LFP +R+P+SAPIR EELR LV+ W LH +RNFW+ + TK+D+V  L +HI  +KV+                                +   T  P  T +    +A    ARP S  + +DS  + I   SR     T+     S G  +  S A +              G D+   R + +   I     A  +  LAL ++ +      A  D   +  P L +++ +      R+   C  ++    L  G+    + EG +  +VR +   +D  V++Y +A                                                             +PAL  +  ++D+ T +     LVNIA    +  ++S+   V R  +     K +     F    + ++++L   RA   DD +     DI       + D   + + A+ N    + +     +  I+     L+ +          +A A  +CT+     L+A  ++  ++   L+  G     +  +N+S+      A+ +VGLS+ A     H   L    ++ +L+  +   +   + H + LLC L++++ ++A +V   V+  V A+ N  T      + LFN+SC   ++  LL       + + L     PS               Q  CL  + N+S  +     L+    +  L A   K       + + A  L N S    +  +T M+D  A+ L   L+ +   K   LC+AT  CN+ I  +      L + LI LS +  A +   CA + + L++    R  L     + P L  MMRSG++D   VQ   A ALC +      K N   + KEG I D I  ++LRI    TKE  A  +FN+L  ED R  +     ++ALV+L RL S ++ ++CV A+YNL+C+  +    L+++    V+          V+ ++   A L  ++   G    L + G + A   +    D + + + A++  +L+ + +   A+A      +L  +     +      +  LCN+S                                              +PA    +E     A+C I++ L + EE+   L  SK L ++    + H+ ++    +P L++  K    +   DV A  L  LS    +   +V  G  EAL   + E             L+ L++     C V+   +                           C   L+ +S+         E  R+  +   L   GD +   NI ++   IT   + E      R G++  ++ L+     +  QIC+ +L  +  ++ E D
Sbjct:    6 SQAARHTQELLEDLERDKELDVIRIFLARFDLFPRQRDPKSAPIRSEELRDLVKYWKLHRQRNFWKNHTTKEDLVRMLYKHIT-VKVLPNEKSP--------------------------LIPETTTPPTTMV---PIAPQSPARPPSGGMAMDS-PSKIRRTSRLTNGTTEG---YSGGVMSKGSPAKMNALGAY--------GGDLFAQRGDYDCGMIYVSRLAPPETDLALQSLVLVAGEESALKD--SVFSPQLNLLDEDSTRREKRLMTECACSLYQLTLEPGHEADIVREGCIPAIVR-MCTFDDLDVKKYCSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPALISMLNNTDLETKEACVKTLVNIASFSGSVVSESVTHTVTRIAA-----KKDTAYDRFIVETICDMSLLTGPRAKAADDGILLPIFDI--NRNSSNVDLKRMMAIALSNFSGIETNHQHMVNGRILHALDSLLGVDDDEIKVMAATAVANIACTLEFIPKLMASHAEPFNLPLRLIQSGYNATDVLQENISA------ALLSVGLSHQA-----HRLHLTQNGVVLLLIHFLETSNATTKRHAVVLLCALMADDLSRAQLVQHDVLKVVVALANNPTTRELCSVALFNLSCFADMSPYLLAPQTMDALALLLSPPPAPSTSKSADAADVTLSITQEFCLNCLYNLSFYDGSAEVLISSHSVATLGAIFRKPSKNADANLRAATALCNMSFCPDAAHITIMLDDDALKLLRRLATNATTKELVLCIATTLCNLAIQALQTSGPLLVNQLIELSHTPHAEIAFVCAISFSKLASNSILRESLAKVMDLPPTLTVMMRSGIED---VQIHCAAALCGLACERGQKGNR-HMWKEGTITDFIVNSLLRINSDSTKEVCARVLFNVLTHEDCRGHMIKDGVLYALVKLARLDSVEIRSLCVTALYNLSCDDAMVSV-LMDINVAHVIAKMCEGEFNQVENRRRLAACLTNIALQPGHELKLMENGGLTAVLLLCDHGDVECMRYSASVLCSLATQPANCDAMAGIPALELLLKMTNSRDSYQCLFALHALCNISC---------------------------------------------VPALHDKIEE--AEAICSIIRVLGEAEEEDIMLTCSKILCNLAFHAKHHATILKHNYVPVLLQGLKKTKFQSVADVNARILATLSEDAAVVELLVAGGAVEALHIASREGDKATTVVQCVISLVRLSRGTLGGCKVIQDGLFDILQAAIPLPYDPKMHPKLGADLTERCSMILRTLSTFPVCIPDLVEDPRLMPLAIALTEDGDKDTTKNIIMLLHNITAARSREFQRQVRRSGVIPLLIKLARVCTTDELQICAVSLAHINSELSEAD 1318          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A6G0WB74_9STRA (Vacuolar protein 8 n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WB74_9STRA)

HSP 1 Score: 162 bits (409), Expect = 2.710e-36
Identity = 289/1324 (21.83%), Postives = 519/1324 (39.20%), Query Frame = 1

Query:   22 RCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIK---HMKVVHQNMEDKKAERREADRKRLVQ----SAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISKLVEGGLVN---VVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRV--------------------------------VVRTTSALLLE-KCNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKL---------GPDSSDTVILCSAAIFNCLTFKQSRIRATD---------------KSIVKECRRLMSICSA---DAQHSCTVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAMSAVGLSNLAADPGNHWRVLESENMLNM--LLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRNRD----THASAIGLILFNISCNPKLAGSLLDDGLTVPMLVDLMKKHGPSVQAACLGVMQNVSTNNAFHRRLLEQGV----LEALDAAKDVDGGALSAQ----CAAVLYNFSLFEQSLTHMVDLGAI-----FLATHLSY------SNVIKTKQLCVATFCNMTIHKVIVEESFLSSLIFL------STSTEASLVMC-------CAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLV-KEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQD--VTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQENTSSRAAFGLLAGTMRAGSLYLTTRL--DALRTM---------------VNLVTHYLPARQTAVETHVTSALCIILKALIDEEEKLLVSKALRDMTLFTQGHSQMMTDGVLPALVRLAKVE------------------NAEIKQDVATALCRLSASLELAFDIVDEGLAEALFWLTLED 3555
            +CA   ++R     ++   + LR +L K  LFP  R+P ++PIR+EEL+ LV+ W LH +R FWR++P KDD+V AL +HI+   + K   Q+ +DK  +  + D          S   E  +  +++   +  +    Y +G                   + G+IY+SR  +   +   + SE  E L   AD E    A  P+     RD   + + S   K+    KC+  L N++         L E G +  ++ ++  N  VD   V L                          V+  L+   +   +                                               L+N+++   +  +D++                                   VV   + L L+  C+   +  C LA   L  L  VRA  D  V   A+ +L +L               T    +AA+ +C +  Q+ +   D               K++ K+   L++   A   D    C + L+  S    +   ++  G +      L      +     + LSNLAA       ++    ++ +  L+ A   D  V Q  ++ L C L+ +  T   +V  GVV A+ A  + D      +                             L D   K    ++AACL  +  +ST+      L  +G     + AL      D  ++S +    C A+L N + +E     +VD GAI     FL TH +       +  +   Q   A+  +      ++   + ++L+ L      S+S+ +   +C       CA A A +S   +GR  L N+ +V P L AMMR+G  +    Q  +AIALCN+    + +  +   +  +  I D I V +LR+   +TK   A  +FNLL  EDTR+ +     ++AL++L +L  P++  +C+RAIYN++ E+   Q +L+++E  R+L T     +     + +CG  L+ +S+V G    L ++G +    +++ ++DP+T  H A    NLS  +     +   D  + ++L +L       +++     + NLS+S +  +  +     G L   +   S  +T  L   ALR +               V +++H + A +T    H  +A  +   AL  +E  ++ ++ L  +   +    Q++ DG++ AL  ++K++                   A  K D+ ++   LS +      ++ +G+ EA+  L L+D
Sbjct:    5 KCAQNMEQRLAALRRDQEIDGLRVYLIKYNLFPRNRDPHNSPIRVEELKELVKHWKLHRQRGFWRDHPEKDDLVRALLQHIRTEANSKKRRQDAQDKYRKASDGDEXXXXXXXXXSVDQERHHLSKMSEGVKPFTGDLFYTRG-----------------HYDEGLIYLSRMEKSKQQRHKMHSEQNE-LRVVADKE----ADMPIHL---RDKESTANLSKDMKL----KCAEGLYNVSCYANNEMQMLSE-GTINIIVGLLK-NLGVDDTHVRLXXXXXXXXXXXXXXXXXXXXXXXXXPVLLELAHANNMTTKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLINLSVIPRSTSSDTVMSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVVEDGAMLALKILCSYQQVPVCFLASCVLCNLAAVRANQDSMVKNGALAVLVELFELPRRRSTASHPETTSSSSAAAMGSCPSISQA-VAGGDAPPPFNLLELYGRKAKALKKQADDLVTADEATMLDIHIQCAITLSYFSCNTKLQPRIVSAGFVPRLLAMLELRHDDTTKYITLVLSNLAAHDACRVHMVNDGCVVPLIRLMSASYTDMVVKQDCVVAL-CSLMLHPQTYKTMVDDGVVPALVAFSDDDHPEIQKSXXXXXXXXXXXXXXXXXXXXXXXXXXXXNLADRCAKQR-ELRAACLSALFQLSTDLTNAEALFYEGTQTVAITALSEPIATDVPSVSHRMWMHCLALLSNMATYESGRQVLVDDGAIDAVLRFLQTHATSVRDKASARYVSRAQAFAASMLSQLADAAMMHAGYFNALLALASSHSFSSSSSSRCDVCAIVATLRCAVAFAAMSCSTKGRRLLANSSNVSPGLNAMMRTGHHET---QLYAAIALCNIA---MERGAIPDRIWADDTISDFIVVALLRVNSDETKVKCAKVLFNLLTHEDTREKLVVDGVLYALIKLAKLDIPEIRELCLRAIYNISLELSKIQ-RLVDVEIVRILGTMFQGDHAKETKRLVCGI-LSNVSAVPGNEVQLLQEGALAIVASLIKIRDPETRVHCANALSNLSCNEKVAEMILKDDGNIVAILISLSRAESRDIRRFAAGAISNLSASRLGVDVMTREGMIGALTELLHRLSCDVTLALCVRALRNLMVDVENQTKLVTCRGVQILSHLMTATETTDANHANAATAM---ALSRKETGMICAELLCLLCCNSGVEVQLVEDGIIRALTAISKLDLGSTSSNHGASHDHAKAAAASAKLDIISSFSTLSKNPACHDQMIKDGIMEAIVRLCLDD 1283          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A485KQF2_9STRA (Vacuolar protein 8 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KQF2_9STRA)

HSP 1 Score: 159 bits (402), Expect = 1.820e-35
Identity = 248/1110 (22.34%), Postives = 447/1110 (40.27%), Query Frame = 1

Query:   22 RCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKH---MKVVHQNMEDKKAERREADRKRL-VQSAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGIIYMSRWAQDNTKIDDITSEGTE-NLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISK---LVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAI-PALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSM-------------------------------QRVVVRTTSALLLEK--CNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKLGPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHSC-TVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAMS-----AVGLSNLAADPGNHWRVLESENMLNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRN------------------------------------------RDTHAS---AIGLILFNISCNPKLAGSLLDDGLTVPM--LVDLMKKHGPSVQAACLGVMQNVSTNNAFHRRLLEQGVLEAL-----DAAKDVDGGA--LSAQCAAVLYNFSLFEQSLTHMVDLGAI-FLATHLSYSNVIKTK---------QLCVATFCNMTIHKVIVEESFLSSLIFLSTSTEASL-------------VMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLS 2976
            +CA   ++R     ++   + LR +L K  LFP  R+P+++PIR+EEL+ LV+ W LH +R FWR++P KDD+V AL +HIK     K   Q+ +DK  +  +AD +    ++A     +P     + +       Y +G+                    G+IY+SR  +   +   +  E +E  + ++ +L+        L+    +D   + + S   K+    KC+  L N++         L E  +  P+L  +  N   D   V L C   +L      S    +++ G + +V  L+   +   + +                    DG +   L ++A ++D L  +L+   L+N+++   +  +D++                                RV V    A+L  K  C+   +  C LA   L  L   RA  D+ V   A+ +L +L                    F+  + R T      E        S+   HS  T +  +L         LL     Q   D     D  +  +      A+ LS  + +P    R++ S   +  +L  + + H      I  +L  L S+E+ +  +V+ G VG + ++ +                                           D+H     +    L +++ +  +   L+  G+ + +  L D   K  P+++AACL  + ++ST       L  +G          +   DV   +  +     A+L N + +E+    +VD GA+  +   L  +++++ K         Q   A+         ++ ESF ++L+ L+++   S               + CA A A++S   +GR  L NN +V P L AMMR+G  +    Q  +A+ LCN+ +        I    +  + D I V +LR+   +TK   A  +FNLL  +DTR+ +     ++AL++L +L   D+  +C+++IYN+  E    Q +L+EME  R+L T     N     + +CG  L+ +SSV G    L  +G +     ++ ++DP+T  + A    NLS
Sbjct:    5 KCAQNMEQRLAALRRDQEIDGLRVYLIKYNLFPRNRDPQNSPIRIEELKELVKHWKLHRQRGFWRDHPEKDDLVRALLQHIKSEAASKKRRQDAQDKFRKVTDADDQHAKARAALELDPFPSHSPEKGQAVGGDLFYTRGL-----------------YNEGLIYLSRMEKSKQQQHKLQQEQSELRIVAERELD--------LQAAHMKDKDSTANLSREMKL----KCAEGLYNVSCYPHNELQMLAENAV--PILAGLLKNLSGDDTHVRLYCAATLLNLSIMASARAMMIDHGAIPIVLELAHCNNITCKLFXXXXXXXXXXXXXXXXXXXXDGCVLTLLQLMAVANDELK-ELSMKALINLSVVPRSTSSDTVLSXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTRVNVVEDGAILALKILCSYQQIPVCQLASCVLCNLAAARANQDNMVKNGALAVLVEL--------------------FELPQRRFTKAQPHPE--TTTDTTSSHPHHSGGTDVKFDLHGLYHRKAKLL-----QTKPDAAPLSDDEAAMLEIHMQCAITLSYFSCNPKLQPRIV-SAGFVPRVLAMLTMRHDDTTKAITLILSNLASHEACRVQMVSDGCVGPLISLMSASHSDMVVKQDCVVALCXXXXXXXXXXXXXXXXXXXXXXXYSEDSHPEIQKSCAFALLSLTMDKAMKVKLVSQGVILALINLADRCAKR-PTLRAACLCALYHLSTECDNEESLFYEGTQTVAITVLNEPITDVPSVSHRMWMHSLALLSNMATYEKGRAVLVDDGAVDSVFRFLQVNSIVRDKASAMYLARAQSYAASMLCKLCDVAMMHESFFAALLALASTHTVSASSSSRSDISAMITTLRCALAFASMSCSMKGRRLLANNGAVAPGLNAMMRTGHHET---QLYAAVGLCNIATERGATPGRIW--ADDTVSDFIVVALLRVNSDETKVMCAKVLFNLLTHDDTREKLIIDGVLYALIKLAKLDIQDIRELCLQSIYNIALETAKVQ-RLVEMEIVRILSTMFQADNSKETKRLVCGI-LSHVSSVAGNEVQLIHEGALAIITILVKIRDPETRVYCANALSNLS 1046          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A1V9ZVE9_9STRA (Vacuolar protein 8 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZVE9_9STRA)

HSP 1 Score: 155 bits (391), Expect = 3.580e-34
Identity = 248/1141 (21.74%), Postives = 460/1141 (40.32%), Query Frame = 1

Query:   22 RCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHM---KVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLARPVSAELPVDSIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAP-EPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVV---NILTEGYRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTT-------------------------------SALLLEK--CNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKL-----------GPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHS-------CTVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAMSAVGLSNLAADPGNHWRVLESENM--LNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRNRDTHAS---AIGLILFNISCNPKLAGSLLDDGLTVPMLVDLMKK--HGPSVQAACLGVMQNVSTNNAFHRRLLEQGVL--------EALDAAKDVDGGALSAQCAAVLYNFSLFEQSLTHMVDLGAI-----FLATHLSYSNVIKTKQLCVAT------FCNMTIHKVIVEE-SFLSSLIFLSTSTEAS-------------LVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNV------LSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVL--VTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQD--VTSVLAALHEIGQTIVKKICVATLCNLSSSSVAQE 3120
            +CA   ++R     ++   + LR +L K  LFP  R+P ++PIR+EEL+ LV+ W LH +R FWR++P KDD+V AL +HIK     K   Q+ +DK  +  E D  +   +   +G+  Q +A   +  +    Y +G                 + + G+IY+SR         D + +  + L S +D   K T   + L +G   +V   +D S       + KC+  L N++         L E  + P +  ++     +D   + L C     N+         ++E G ++ +  L+   +   +                               L    +    +LA   L+N                                                     A+L  K  C+  ++  C LA   +  L  +RA  D+ +   A+ ++ +L            P++ +T        F+   F   + +  D + + +      +   D +++       C + LA  S    +   ++  G +      L      +     + LSN A+      +++    +  L  L+ A   D  V Q  ++ L C L+ +  T   +V  GVV A+ A  + D H     +    L +++ +  +   L+  G+ +  L+ L  +    P ++AACL  + ++ST+    + L  +G          E L+  +      +     A+L N + +E+    +VD GA+     FL  + +  + +  + L  A        C ++  +V +E   + S+L+ L++S   +               + CA A AN+S   +GR  +G +  V   L AMMR+G  +    Q  +AI LCN+      L   +  DN +S        D I V +LR+    TK   A  +FNLLA +DTR+ +    A++AL++L +L   ++  +C+R+IYN++ ++   Q +L+EME  R+L  + Q  +     D+K++    L+ +SSV G    +  +G +     ++ V+DP++  + + +  NLS        L   +  V  +L +L       +++     + NLS+ S+  E
Sbjct:    5 KCAQNMEQRLAALRRDQEIDGLRVYLIKYNLFPRNRDPRNSPIRIEELKELVKHWKLHRQRGFWRDHPEKDDLVRALLQHIKTEAANKKRRQDAQDKFRKINEIDEHQQESAQEEDGKPMQMLAETAQTSNGDLFYTRG-----------------NYDEGLIYLSRM--------DKSKQRQKLLNSASDTSLKTTTNVQHLTKGLLANVEHEKDVSTNFSKDMKVKCAEGLYNVSCYHNNEMQMLAENAI-PIIAGLIKNPPTIDDTHIRLYCAAALQNLAMSHQARDLMMEQGAISTILELAHTNNGTTKLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQLMSLPNEEIKELAMKSLINXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVEDGAILALKILCSYRNVQVCQLASCVVCNLAALRANQDNMIKNGALLVIMELFNLPQFHRKQTSPETQNT--------FDYTRFYAKKAKLLDPNYIGD------LAFTDDENAMLEIHMQCAITLAYFSCNSKLQPRVVGAGFVPRLLALLGMRHEETTKYITLSLSNFASHESCRVQMVREGAVRPLITLMSAPYADMLVKQDCVVTL-CNLMLHPETYKEMVDDGVVPALVAY-SEDNHPEIQKSCAFALLSLTMDTSMKPKLVQQGV-ITALISLADRCAQRPELRAACLCALYHISTDVQSAQALYYEGTQFLAVAVLNEPLNDKQPNISHRMYMHSLALLSNMASYEKERNALVDDGAVDAVLRFLQANSTVKDKVSAQYLARAQAYASSLLCKLS--EVAMEHPGYFSALLVLASSANITPNSSNRNDVTGIITTLRCALAFANMSCSMKGRRLMGKHGEVAIGLNAMMRTGHHET---QICAAIGLCNLAIERGPLKGQVWADNTVS--------DFIVVALLRVNSDTTKVICAKVLFNLLAHDDTREKLIVDGALYALIKLAKLDMEEIRQLCLRSIYNISLDLGKVQ-RLVEMEIVRILCGMHQQDYSK---DMKRLICGILSNLSSVPGHELQMINEGALTIISTLVKVRDPESRVYCSNVLSNLSCNPDVLELLLKDEGNVIGILISLTRAESKDIRRYSAGAISNLSTVSMGIE 1085          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: A0A418AQI7_9STRA (Vacuolar protein 8 n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A418AQI7_9STRA)

HSP 1 Score: 146 bits (368), Expect = 1.800e-31
Identity = 237/1041 (22.77%), Postives = 424/1041 (40.73%), Query Frame = 1

Query:   22 RCAALAQERYCRFPKEGRTEVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASRTEGPSNTFLYHQG-VALPRLARPVSAE---LPVDSIETGIIYMSRWAQDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDESNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISK---LVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPAL-NVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKCNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILA---KLGPDSSDTVILCSAAIFN----CLTFKQSRIRATDKSIVKECRRL---MSICSADAQHSCTVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAM------SAVGLSNLAADPGNHWRVLESENMLNMLLRAVVLDHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQAMRNRDTHASAIG----LILFNISCNPKLAGSLLDDGLTVPMLVDLMKKHGPSVQAACLGVMQNVSTNNAFHRRLLEQGVLEAL-----DAAKDVDGGALSAQCAAVLYNFSLFEQSLTHMVDLGAIFLATHLSYSNVIKTKQLCVATFCNMTIHKVIVEESFLSSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMM-------RSGVKDAAQVQFLSAIALCNVLSVF-LHKDNVISLVK---------------EGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLS 2976
            +CA   ++R     ++   + LR +L K  LFP  R+P+++PIR+EEL+ LV+ W LH +R FWR++P KDD+V AL +HI+           KK  +   D+ R V       Q  Q  A  T   S + L   G +   R+    S           + G+IY+SR  + + ++  +  E  ++L   A+ E    A         +D   + + S   K+    KCS  L N++          +E  +L P  +    N   D + V L C   +L      +    +V+ G + +V  L+   +   +   A  L   S    +   LV DG + AL  ++A + D L  +L    L+N+++   +  +D+     V +T                             R  + +  A +A+ IL    ++      + +LC+ A       C+  K   +    +      RRL       + +   S T   A    +   T   L    +Q    +L +  P    M       A  LS  + +     R++ S   +  +L  + + H      +  +L  L S+ES + ++VA G V  + ++ + +     +     + L N+  +P+    ++DDG+ VP LV   +   P +Q +C   + +++ +     +L+ QGV+ AL       A  VD   L A C   L+  S    +            AT L Y       Q    T  N  I                  +    + M     L+N++TY +GRS L ++ +V   L  +        ++  +  A+ Q  +A  LC +  V  +H     SL+                +  + D I V +LR+   +TK   A  +FNLL  +DTR+ +     ++AL++L +L+   +  +C+++IYN++ E+   Q +L++ME  R+L T     +     + +CG  L+ +S+V G    L  +G +    +++  +DP+T  + A+   N S
Sbjct:    5 KCAQNMEQRLAALRRDQEIDGLRVYLIKYNLFPRNRDPQNSPIRIEELKELVKHWKLHRQRGFWRDHPEKDDLVRALLQHIR------TEASSKKRRQDAQDKFRKVNGDLDIDQQ-QANAMTTSASSPSLLSPSGQINEMRMGDKTSTGDLFYTRGHYDEGLIYLSRMEK-SKQVHKMQHEHNDDLRVVAEKELDLLAHM-------KDKEATANLSKDMKL----KCSEGLYNVSCYPN------NESQMLGPFTDSSRRNLASDDVHVRLYCAATLLNLSMTPASRALMVDQGAIPLVLELAHANNITCKLCCAHTLFRFSGDEDIHFHLVHDGCVMALLQLMAVAHDELK-ELCMKALINLSVIPRSTSSDT-----VLSTXXXXXXXXXXXXXXXXXXXXXXXXXXXTTRVNVVEDGAILALKILCSYQQIQVSELASAVLCNLAAVRANQECMV-KNGALAVLVELFELPQRRLGKPHQAATPEGSSSDTGSGAPFDLHALYT---LKAKWIQNECASLPALTPDQSVMLDIHMQCATTLSYFSCNTKLQPRLV-SAGFVPRVLGLLAMRHDDTTKTVTLILSNLASHESCRVHMVADGCVRPLISLMSSNNVDMVVKQDCVIALCNLMLHPQTYKEMVDDGV-VPALVAYSENPHPDIQKSCAFALLSLTMDTGMKAKLVGQGVIVALINLADRCASRVD---LRAACVCALFQLSTDPDN------------ATALFYDGT----QTVAITVLNEPISPT-------------QPAVSHRMWMHALALLSNMATYDKGRSVLVDDGAVDAVLRFLQAHTNVRTKACARYVARAQSFAASMLCKLRDVASMHSGYFASLLALASTHRGATPDRIWADNTVSDFIVVALLRVNSDETKLKCAKVLFNLLTHDDTREKLIVDGVLYALIKLAKLEIDTIRELCLQSIYNISLELGKVQ-RLVDMEIVRILSTMFQTDHSKEMKRVVCGI-LSNISAVAGNERQLVHEGALAIIASLVKARDPETRVYCASALSNFS 974          

BLAST of mRNA_H-paniculata_contig2162.5705.1 vs. uniprot
Match: F0WI41_9STRA (Vacuolar protein 8 n=1 Tax=Albugo laibachii Nc14 TaxID=890382 RepID=F0WI41_9STRA)

HSP 1 Score: 144 bits (363), Expect = 7.050e-31
Identity = 239/1068 (22.38%), Postives = 407/1068 (38.11%), Query Frame = 1

Query:   79 EVLRPFLTKLGLFPPRRNPESAPIRMEELRALVRKWNLHHKRNFWRENPTKDDVVTALNRHIKHMKVVHQNMEDKKAERREADRKRLVQSAFGEGQYPQRVASRTEGPSNTFLYHQGVALPRLAR---PVSAELPVDS----------------IETGIIYMSRWA--QDNTKIDDITSEGTENLASKADLEDKNTAPEPLEEGDGRDVGGSRDE--SNAKKIQAQQKCSLALLNMTMRDQMSKAFLDEYGLLPPLLEIMNVNQVVDVLRVGLACVVNILTEGYRISKLVEGGLVNVVRPLSGHEDEQVQQYVAAILLAISACPGLEEWLVQDGAIPALNVLARSSDILTAKLAAGGLVNIAITLTAAQADSMQRVVVRTTSALLLEKCNGDSLHFCALAVKNLTVLDNVRAYLDDQVAAIAVDILAKLGPDSSDTVILCSAAIFNCLTFKQSRIRATDKSIVKECRRLMSICSADAQHSCTVLLAELSKYRDVTNNLLDGGIMQLFSDNLSSFDPRSVAMSAVGLSNLAADPGNHWRVLESENMLNMLLRAVVL-------DHTVAQHHILCLLCGLVSNESTQANVVAAGVVGAVQ--AMRNRDTHASAIGLILFNISCNPK-----LAGSLLD-------------DGLTVPMLVDLMKKHG---------PSVQAACLGVMQNVSTNNAFHRRLLEQGVLEALDA--AKDVDGGALSAQCAAVLYNFSLFEQSLTHMVDLGAIFLATHLSYSNVIKTKQLCVATFCNMTIHKVIVEESFLSSLIFLSTSTEASLVMCCAKALANLSTYPRGRSFLGNNKSVVPALIAMMRSGVKDAAQVQFLSAIALCNVLSVFLHKDNVISLVKEGLIQDLIAVTVLRIEEVKTKETLAHAIFNLLAREDTRKLIADQDAVFALVRLTRLQSPDLNAICVRAIYNLTCEMPIYQPKLLEMEAERVLVTQASFPNGGVDVKKMCGAALAMMSSVKGASCALAKQGVVGAFRAIMCVKDPDTLEHIATIAFNLSREDSCRAALAAQDVTSVLAALHEIGQTIVKKICVATLCNLS 3099
            +VLR +LT+  LFP  R+P SAPIR EEL+ LV+ WNLH +RNFW+ + T+DD+V  L+ +I       +  EDK+      ++ R  +   G  +  + +A     P+       G    R      P S E P  +                 E G+IY SR +  Q+ TK   + +E T     K + +DK+ + +   EG G D     +   S A++I  +++C+  +   T      +  ++E  L    +  + +   ++V                                                                             +DI   +     L+NIA    +   DS   VV   T    +E+       F +L V NL++L   RA + D+     +  L+    DS  +  +  A + N      +     D  +++  R L+         +CT+ L                                            A+        L++E  L   L  +            +AQ + L  LC LVS +     ++    + A+   +++  D        +  NIS +       + GS++D             DG+ +   +  +   G         P  Q   LG + N+S + A   +  E  +++ L     +        + CA +L N +       H +++G + L   L +SN   T   C    CN+   + + + + LS +I LSTS   +++  CA AL  L+   + RS L     V P LI+MMRSG ++   VQ  SA  LCN+      +   I    EG + + I   +LR+    TKE  A A++NLL  ++ R        ++AL++L+RL S  +  +CV A+YNL+CE  + +  L+E+   +V+           D++++  A+L  +SS      +L + G + A   ++      T+ +      NLS E S   A+   DV+  L  L   G      + +  LCN+S
Sbjct:   26 DVLRTYLTRFNLFPRNRDPASAPIRYEELKDLVKHWNLHRQRNFWKNHTTRDDLVRTLHTYINTKAEAREPREDKRKASVTVNKDREFED--GADKAFEMLAHTPSAPNRNNSISLGSPRTRSISKVVPKSPEGPTSNHVFNMYSGDIFTQRGDYENGMIYSSRLSKPQEKTK-SHLQNENT--YLGKDEDQDKDKSSDM--EGMGVDQIALAEHVGSTAREITLKRECAFTIYYFTTIPGNERQMVNERCLFA--IGCLALVDDLEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXADIEAKESCIKALINIASFSGSPIPDS---VVQSLTKMAKIEEMKYK--RFASLVVYNLSLLPASRAKILDEDMLNTIFFLSHSNKDSELSRNIAKA-LRNFSGITSNLDTLGDIKVLETIRVLLKQREVHVIENCTITLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAS--------LQNEAQLKYWLLIIPFFINLFKDGACLAQLYTLTALCYLVSFDEVSTALIQLDFIAALSELSLKASDKTKELCANLYLNISHSLAYHSHLMEGSVIDSILRLLRRTKINKDGVEIDDEITPLSIGGVHEKMPLALPRSQECALGCIYNLSFSEAHCSKFAESTLMKVLITMFTRSTRTEDAISWCAIILANLTFDACCRKHFIEIGGVRLIRKLMHSNTKDTLLCCATAACNLA-GEALEKTAVLSIIIDLSTSQHTTIINTCAIALYKLAIKEQSRSTLAKTPEVFPTLISMMRSGNEN---VQIRSAATLCNLAMEKNARSRHIW--TEGTVPNFIVNALLRVNSAATKEICACALYNLLTHDEHRISHIKDGVLYALIKLSRLDSKRIQILCVDALYNLSCEHTMLE-SLMEVNVAQVMTKICENECIDQDLRRLLAASLMNLSSRSNVRVSLVENGALVAVTKLLHSGVTPTVSYCTATLSNLSSERSNCEAMVTNDVSKSLLNLASSGDQQQSILALNALCNMS 1063