mRNA_H-paniculata_contig10837.535.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: D7FTL9_ECTSI (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTL9_ECTSI)

HSP 1 Score: 1346 bits (3484), Expect = 0.000e+0
Identity = 666/849 (78.45%), Postives = 747/849 (87.99%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVEN-ENVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVT-------EES--DQE-DFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRRESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDLEW 2945
            V Q+PAFGEDDYRVCLAHGVI KGE+IPCPVDSNGLFT EVG YAG+YVK ADKALCDEIK+ GR+ SKDS  HSYPFCWRSDTPLIYKAVPSWFV VESIR++LL +NA++YWVP+FV EKRFQNWLEGAKDWAVSRNRFWGTPMP+W+S+DGEE+VAVGSIAELKELSGVEVTDLHRE VDHITIPSK+GKGDLKRV+EVFDCWFESGSMPYAQLHYPFEN++KFE+NFPADF+AEGLDQTRGWFYTLTVL  ALFDKPAFKNLIVNGLVLASDG+KMSKRLKNYPDPMKV+D+HGADALRLYLINSPVVRAE LKF EEGVQATVR+VLLPWFNAFRFFVQQARRLEM+CG+RFVP+P+AAA S NVMD WI   LQGLV+FV+TEMKAYRLYTVVPRLV FIE+LTNWYVRLNRNRLKG EG ESA  GLFCMYEVL+TMSVLMAP TPFFSEYTY  LR+CH + +  ++VAEDA GRASSVHMLM+P VDESR+DP+ E+DM +MQ VI+LGR AR+KR ISLKTPVKGITVVCK + TL ALEKLQ YVKGELNAWEV LEADEK+WCTLTAEANNKVLGKRLGK +  VKK L KLDTAALWPLLEG ++E+ G TL+ DDLMLKRGF GD  V++AAVSEDGK+MVVLDTR+DEK+L+QK++REIVSRIQKLRKK+GLQVGE VEVFF+D+TK G+AG AVA NA+LVADAVRC+PLP S MP H+VP+G E F          EE+  DQE DFT FLTRPCLS++  A+DAKC +A VD AV  QL+ SLD+DRVRRE   +  LTVGEA VTVDLG +YFLDAR A+R+    D++W
Sbjct:  343 VHQSPAFGEDDYRVCLAHGVIAKGEEIPCPVDSNGLFTSEVGEYAGQYVKTADKALCDEIKANGRLVSKDSCTHSYPFCWRSDTPLIYKAVPSWFVGVESIRDKLLKANAETYWVPAFVNEKRFQNWLEGAKDWAVSRNRFWGTPMPMWVSEDGEEMVAVGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQLHYPFENKDKFEKNFPADFIAEGLDQTRGWFYTLTVLGAALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDSHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCGDRFVPNPEAAAKSTNVMDNWIQATLQGLVQFVHTEMKAYRLYTVVPRLVEFIEQLTNWYVRLNRNRLKGAEGAESATAGLFCMYEVLSTMSVLMAPITPFFSEYTYRHLRECHPDKDGGKDVAEDAPGRASSVHMLMMPEVDESRLDPQAESDMRAMQTVIDLGRSAREKRGISLKTPVKGITVVCKDEGTLKALEKLQGYVKGELNAWEVVLEADEKSWCTLTAEANNKVLGKRLGKALDGVKKALVKLDTAALWPLLEGGTVEVAGHTLSADDLMLKRGFKGDTSVFQAAVSEDGKVMVVLDTRKDEKVLSQKLAREIVSRIQKLRKKSGLQVGETVEVFFQDDTKDGVAGAAVAANAALVADAVRCMPLPASRMPEHAVPLGHERFCACGGDDGKGEEAAADQEPDFTVFLTRPCLSVDHEAMDAKCVAAKVDPAVARQLLASLDYDRVRREGNATTVLTVGEACVTVDLGADYFLDAREALRKRCPEDMKW 1191          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: W7U130_9STRA (Isoleucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U130_9STRA)

HSP 1 Score: 914 bits (2362), Expect = 5.140e-313
Identity = 467/862 (54.18%), Postives = 607/862 (70.42%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVE-VTDLHREFVDHITIPSKKGKG--DLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENENVAE---------DAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNAL-EKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYM-PSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRRESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDL--EWVF*LFTGG 2969
            V QAP FGEDDYRVCLAHGVI KG ++PCPVDSNG FT +V  + G++VK AD  +C  +K+KGR+  K +Y HSYPFCWRSDTPLIYKAVPSWFVAV +++E+LLA+N ++YWVP+FVKE RF NWL  AKDWA+SRNRFWGTP+P+W S+D EE VA+ SI +L+ LSGV+ +TDLHRE VD ITIPS +G     L+RVDEVFDCWFESGSMPYAQLHYPF+N+E+FE NFPADF+AEGLDQTRGWFYTL V+ST LFDKP F+NLIVNGLVLASDG+KMSKRLKNYP P  ++  +GADALRLYLI+SPVVRAEPL+F EE V   ++ V LPW NAFRFF Q   RLEM  G  F P P+ A +S NVMD WI  +LQG++KFV+ EM AYRLYTV PRL+ FIEELTNWYVRLNR RLKG EG E + TGL  +YEVL +M++LMAPFTPF +E+ Y  LRK H N  + + A          DA G+A SVH +M+P  DE+R++P  E  MA++Q VI LGR  R++ +I+LKTPVK + VV K Q  L  L E ++ Y   ELN W +K   +   W  +  E NNK+LGKRLGK    V   +A L    +       S+ + G  L+ ++L+LK+ F GD  +++A  S+DG L+VVLDTRQDE++LAQ  +RE+V+R+QKLRK+ GL++ +VVE+++E   KG +  +A+A NA++V   +R  PLP S + P+H+V +G+E   + + +     T ++TRP LS+ + ++           A  +  M S+D+DR+R      ++  +    V++D G  YF    +A+ ++ +R L  E VF    GG
Sbjct:  345 VHQAPGFGEDDYRVCLAHGVIRKGTNVPCPVDSNGRFTPDVRDFVGQHVKAADGDICQLLKAKGRLVDKATYSHSYPFCWRSDTPLIYKAVPSWFVAVGTVKEKLLANNQKTYWVPAFVKEHRFHNWLADAKDWAISRNRFWGTPIPLWCSEDMEEQVAIESIEQLERLSGVKGITDLHRESVDQITIPSSRGPSYPPLRRVDEVFDCWFESGSMPYAQLHYPFDNKERFEANFPADFIAEGLDQTRGWFYTLMVISTCLFDKPPFQNLIVNGLVLASDGKKMSKRLKNYPPPDDILKKYGADALRLYLISSPVVRAEPLRFKEEDVLGMIKRVFLPWINAFRFFQQGVARLEMEKGSVFTPSPERARASTNVMDVWIQASLQGMIKFVHDEMAAYRLYTVTPRLLRFIEELTNWYVRLNRTRLKGQEGDEESLTGLSTLYEVLLSMTILMAPFTPFLTEFLYQHLRKIHPNFTSCSAAASSSVSALPLDAIGKADSVHYIMLPMYDEARLNPLCEERMATLQTVIELGRMVRERSNINLKTPVKEVIVVSKDQGKLAHLDESVRGYALQELNVWSLKTSDEVGKWSNVHVEPNNKLLGKRLGKDRAKVAAAIAALTPEDIEAFQNTGSLTLAGYELSSEELLLKKEFKGDTSIFQAEASQDGTLLVVLDTRQDERVLAQATARELVNRVQKLRKRVGLKLSDVVEIYYETEDKGMI--EAIASNAAMVRSTLRVFPLPASTLKPTHAVELGKEECLINDVA----VTVYVTRPTLSLCDESILQAVGGEQERAAGASAFMASMDYDRLREGDTRGVTFMLDGHKVSLDAGVHYFF---SAMEKAQARALLPEMVFLSVNGG 1197          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: M4BBV1_HYAAE (Isoleucine--tRNA ligase n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4BBV1_HYAAE)

HSP 1 Score: 912 bits (2358), Expect = 8.320e-313
Identity = 465/848 (54.83%), Postives = 593/848 (69.93%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVEN---ENVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFED-NTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRR-----ESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDLEWV 2948
            V QAP FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G +VK+AD  +C ++K++GR+ SK +  HSYPFC+RS TPLIY+A+P WFV VE IR R++A+N  +YWVPSFV+EKRF NWL   KDW +SR RFWGTP+P+W+S+D EEVV VGSIAEL+EL+G +VTDLHREF+DH+TIPSKKGKG L+RV EVFDCWFESGSMPYAQ HYPFEN+EKFE  FPADFVAEGLDQTRGWFYTL VLSTALFDKPAFKNLIVNGLVLA DGRKMSK LKN+ DP +++  +GADALRLYLINSPVVRAEPLKF   GV   +R++ LPWFN+ RF+ QQA RL++  G  FVPD  AA +S NVMD WI+ AL  L+KFV+ EMKAYRLYTVVPRLV FI +LTNWYVRLNR RLKG  G+  A   L  +YEV   ++ +MAPFTPFF+EY Y  LR+ H NV N    ++AEDA G + SVH LM+P  D S VD   E  M ++Q+V+ +GR  R++R+ISLK PV  + VV   Q+TL+ L +LQ Y+  ELN  ++    DEK WC L AEAN++ LG+RLGK + +VKKE++++    +   +   S+ I G  L GDDL++KR F GD +VYEA VS  G LMVV+DTR+DE+L  Q  +RE+++R+QKLRKKAGL V + + V+FE+ N   G   KA+  +  ++A  +   P PVS  P+HSVP+  E     + S +      + RP +      + AK   A++ V   T  + S+ ++ V+      E+  ++  T  E T+  DL  E FLDA A  +      L W+
Sbjct:  333 VHQAPTFGEDDYRVCVREGVTDKFT-LPDPLDDNGIFTDAVSLVKGLHVKKADDVICQDLKTRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRNRIVANNKLTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSEDYEEVVCVGSIAELEELTGEKVTDLHREFIDHLTIPSKKGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKEKFEATFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKNFTDPEEILQKYGADALRLYLINSPVVRAEPLKFQASGVLGVIREIFLPWFNSARFYAQQATRLQLETGMAFVPDRKAALASTNVMDFWIIAALHNLIKFVHVEMKAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEAAVALSALYEVEYNLAKIMAPFTPFFTEYMYQFLRQFHPNVVNGAGADLAEDADGVSPSVHFLMLPDFDSSHVDEEVEVLMQNLQSVVEMGRVVRERRAISLKNPVMKVIVVSSDQKTLDGLRRLQTYLHDELNVRDLDFSTDEKEWCVLKAEANSRALGRRLGKALSNVKKEISQMTHDDVAAFVSNGSVTIDGHELTGDDLLVKREFKGDTKVYEAGVSPKGDLMVVIDTREDEQLKMQGCAREVITRVQKLRKKAGLVVQDKIHVYFEEKNGDEGPISKAIQSSLPMIASTLGTAPAPVSLQPAHSVPIVSEDAQFADSSVK----LIVARPAVLFASAEVLAK-HEAAMPVEEFTVYVASMKYEDVKTALESTENSVTVRSTTAEVTLKADL--EVFLDASALAKTLDVPKLSWL 1172          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A8K1FDR5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FDR5_PYTOL)

HSP 1 Score: 910 bits (2353), Expect = 4.270e-312
Identity = 461/844 (54.62%), Postives = 592/844 (70.14%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENE--NVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRR---ESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDLEWV 2948
            V QAP FGEDDYRVC+ HGV DK  D+P  +D NG FT  V    G +VKEAD  +C +IK +GR+ SK++  HSYPFC+RS TPLIY+AVP WFV VE IRER++ +N Q+YWVPSFVKEKRF NWL   KDW +SR RFWGTP+P+W+S+D EEVV +GSI EL+ELSG +V DLHREF+DHITIPSK+GKG L+RVDEVFDCWFESGSMPYAQ HYPFEN+EKF+  FPADF+AEGLDQTRGWFYTL V+STALFDKPAFKN+IVNGLVLA DGRKMSK L+NY DP ++ + +GADALRLYLINSP VRAEP+KF++ GV + +RD+ LPW+N+ RFF Q A RLE++ G+ FVP  + A SS NVMD+WI+ AL  ++KFV+TEMKAYRLYTVVPRLV FI++LT WYVRLNR RLKG EG + AR  L  +YEVL T+S LMAPFTPFF+EY Y  LR+ H NV N    +AEDA G A SVH LM+P  D SRVD + E  M ++Q+VI++GR  R++R+ISLK PV+ + VV     TL+ L  L+ Y+  ELN  +++   DEK WC L AEAN+K LG+RLGK +  V+K +  L    +   L  ++I I G  L GDDL +KR F GD ++YEA VS +G LMVVLDTR+DE+L  Q  +R+ ++R+QKLRKKAGL V + ++V+F +         A+ E  S+    +   P P++  P+HSV +  E    +  S     +  +TRP +     A D   +++ V VA     + S+++  V+        ++S  +     T   G+  FLDA+     S   +L+W+
Sbjct:  332 VHQAPTFGEDDYRVCVKHGVCDKF-DLPNSLDDNGRFTDAVPFVKGLHVKEADDIICKDIKERGRLVSKNTIVHSYPFCYRSGTPLIYRAVPGWFVNVEKIRERIVENNRQTYWVPSFVKEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSEDFEEVVCIGSIKELEELSGEKVEDLHREFIDHITIPSKQGKGTLRRVDEVFDCWFESGSMPYAQQHYPFENKEKFDAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNVIVNGLVLAEDGRKMSKSLRNYTDPNEIFERYGADALRLYLINSPGVRAEPIKFVDSGVLSVIRDLFLPWYNSARFFSQSATRLELATGKTFVPSSEEALSSKNVMDSWIIAALHNVIKFVHTEMKAYRLYTVVPRLVEFIDQLTKWYVRLNRPRLKGSEGNDEARIALSSLYEVLYTISKLMAPFTPFFAEYMYQFLRQFHPNVVNNVAGLAEDADGVAKSVHFLMVPDYDASRVDEQAEQSMKNLQSVIDMGRVVRERRTISLKNPVRKVVVVSSDAATLDKLRALKTYIHDELNMRDLEFTEDEKEWCVLKAEANSKALGRRLGKALSGVRKSIDALSHDEVNSFLLNKTITIDGHELTGDDLFVKREFKGDKKIYEADVSTEGNLMVVLDTREDEQLKQQGCARDFITRVQKLRKKAGLVVQDKIQVYFAEQEGNSTISTAIKEFLSMATTTLGTTPAPLALKPAHSVTIIEEEAQFSGSS----VSIVVTRPAVFF--AADDVLAKASKVAVADAKAYVASMEYADVKTALTSEAAAVSFQILAEKSTFKHGEHVFLDAKEFAAASKQPELQWL 1168          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A482S0Q8_9ARCH (Isoleucine--tRNA ligase n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S0Q8_9ARCH)

HSP 1 Score: 906 bits (2342), Expect = 2.630e-311
Identity = 452/833 (54.26%), Postives = 592/833 (71.07%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENENVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGG-------LAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRRESKPSLSLTVGEATVTVDLGKEYFLDARA 2909
            V QAPAFGEDDYRVC+A+ ++ KGE+I CPVDSNG FT EV  ++G+ VKEAD  +C  +K+KGR+  K+ Y H YPFCWRS+TPLIYKAVPSWFV VE +R +LLA+NAQ+YWVP+FV+EKRF NWL  AKDWA+SRNRFWGTP+P+W+SDD EE+VA+GS+ EL+ LSG ++TDLH+EF+D ITIPS+KGKG L+R+DEVFDCWFESGSMPYAQLHYPFEN ++FE+ FPADF+AEGLDQTRGWFYTL V+ST LFDKPAFKNLIVNGLVLA+DG+KMSKRLKNYPDP+ VV+ +GADALRLYLINSPVVRA+ LKF E+GV   +R V++PW+N+FRFF Q   R E S G+ FVP+   A SS N +D WIL A  GLV+FV+ EMKAYRLYTVVPRLV FI+EL+ WY+RLNR RLKG  G E A  GL  +YEVL TM+V+M+PFTPFF+E+ Y  LRK H N  N+    D FG+A+SVH +M+P  D+S ++PR      ++Q  + L R AR+KR I    P+K +TVV    E + ALE L+ Y   E+N W+++L  D +  C L    N K LGKRLGK++ +V K +++L    +   ++   I++CG +L  +DL++KR FNGD + YEA VS+DG L+V +DT  DE+L  +   R +VS  QKLRK +GL     VE+F+E     G       L   A+  +   V   ++ +PLPVS+ P+HSV VGRE+    + S +  F  +LTRP +S++  AL A          ++   + S+++ +   E+  S+++ +     T+  G  YF  A +
Sbjct:  275 VHQAPAFGEDDYRVCIANNIVGKGEEIVCPVDSNGRFTNEVPEFSGRAVKEADNDICALLKNKGRLVLKEVYAHQYPFCWRSETPLIYKAVPSWFVTVEKLRPQLLANNAQTYWVPNFVQEKRFHNWLADAKDWAISRNRFWGTPIPLWISDDLEEMVAIGSVEELERLSGAKITDLHKEFIDPITIPSQKGKGVLRRIDEVFDCWFESGSMPYAQLHYPFENADRFEKGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYPDPVLVVNNYGADALRLYLINSPVVRADVLKFQEDGVSEVLRGVMIPWYNSFRFFTQCVDRWEGSTGKTFVPNAQLARSSQNDVDIWILAASLGLVEFVHQEMKAYRLYTVVPRLVEFIDELSKWYLRLNRFRLKGEMGDEDAAVGLNVLYEVLVTMTVIMSPFTPFFTEFLYQHLRKLHPNYTNDQAGVDVFGKAASVHFIMLPEADKSLLNPRAVERFKTLQLSVTLARMAREKRRIRTNLPLKSVTVVAARPEDVEALEYLKSYFLAEVNTWQIELSTDWEKMCQLKVMPNWKDLGKRLGKQMKEVAKAISELTFNQISNFMKEGVIDVCGFSLTTEDLVVKREFNGDAKKYEACVSDDGSLLVAIDTTCDEELFCELRMRSVVSAFQKLRKSSGLTPANRVEMFYEVVKAEGQEVEGQLLVDTALNVHRDSVLKRLKQMPLPVSHRPAHSVLVGRENLEEPDVS-KGTFVVYLTRPQVSVDFDALTALVGGNKQTAEMIRMYLCSVEYSKA--ETASSMTVNLEGTAFTLQQGTHYFSSAES 1104          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A2D4BRB4_PYTIN (Isoleucine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BRB4_PYTIN)

HSP 1 Score: 902 bits (2331), Expect = 8.400e-309
Identity = 462/843 (54.80%), Postives = 591/843 (70.11%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENE--NVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRR--ESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDLEWV 2948
            V QAP FGEDDYRVC+  GV+DK E +P P+D NG FT  V    G +VKEAD  +C+ +K K R+  K    HSYPFC+RS TPLIY+AVP WFV VE IR+R++ +N Q+YWVPSFVKEKRF NWL   KDW +SR RFWGTP+P+W+S+D EE+V VGSI EL+ELSG +VTDLHREF+DHITIPS++GKG L+RV+EVFDCWFESGSMPYAQ HYPFEN+E FE ++PADF+AEGLDQTRGWFYTL V+STALFDKPAFKNLIVNGLVLA DGRKMSK L+N+ DP ++++ +GADALRLYLINSPVVRAEPLKF++ GV   +R++ LPW+N+ RFF Q A RLE + G+ FVP    A +S NVMD+WI+ AL  L+KFV+TEM AYRLYTVVPRLV FI++LT WYVRLNR RLKG EG + A   L  +YEVL T+  LMAPFTPFF+EY Y  LR+ H NV N+   +AEDA G A SVH LM+P  D SRVD   E  M ++Q VI +GR  R++R+ISLK PV+ + VV     T++ L  L+ YV  ELN  +++   DEK WC L AEAN+K LG+RLGK +  V+K +  L    +   L+ +SI I G  L+GDDL++ R F GD ++YEA VS +  LMVVLDTR+DE+L  Q  +RE ++R+QKLRKKAGL+V + + VFF++         AV E A++VA A+  +P  ++  P+HSV +  E       S     +  + RP +     A+ AK  +  V V      + S+D+  V+    S  +LS  V E T T+  G+  FLDA+     +   +L+W+
Sbjct:  331 VHQAPTFGEDDYRVCVKQGVVDKFE-LPNPLDDNGRFTDAVPFVKGLHVKEADPIVCNLLKEKDRLIRKGQIVHSYPFCYRSGTPLIYRAVPGWFVNVEKIRDRIVENNRQTYWVPSFVKEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSEDFEEIVCVGSIQELEELSGEKVTDLHREFIDHITIPSRQGKGQLRRVEEVFDCWFESGSMPYAQKHYPFENKEAFEASYPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAEDGRKMSKSLRNFTDPTEILERYGADALRLYLINSPVVRAEPLKFVDSGVMGVIRELFLPWYNSVRFFSQNATRLEETTGQAFVPSSAEALASKNVMDSWIIAALHNLIKFVHTEMAAYRLYTVVPRLVEFIDQLTKWYVRLNRPRLKGSEGNDDAHVALSAVYEVLYTLCKLMAPFTPFFAEYMYQFLRQYHPNVVNQVAGLAEDADGVARSVHFLMVPDFDASRVDENVETCMKNLQCVIEMGRVVRERRTISLKNPVRKVIVVSSDAATVDGLRNLESYVHMELNMRDLEFTTDEKQWCVLKAEANSKALGRRLGKALSGVRKAVDALSHDEVNAFLQNKSITIDGHELSGDDLIVIREFKGDKKIYEANVSLESNLMVVLDTREDEQLKQQGCAREFITRVQKLRKKAGLEVQDKIHVFFQEADGQSTITAAVKEFAAMVAGALGSVPALLALKPAHSVTIISEEAQFAGSS----LSIVVARPAVLFAADAVVAK-GAGKVAVEDAKAFVASMDYADVKTALSSSGALSFRVLEETATLKQGEHVFLDAKELAVATKDAELQWL 1167          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: W2Q337_PHYPN (Isoleucine--tRNA ligase n=29 Tax=Peronosporaceae TaxID=4777 RepID=W2Q337_PHYPN)

HSP 1 Score: 897 bits (2317), Expect = 1.260e-306
Identity = 456/847 (53.84%), Postives = 592/847 (69.89%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVEN---ENVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLT--RPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRRESKPSLSLTVGEATVTVDLGK---EYFLDARAAVRQSGSRDLEWV 2948
            V QAP FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G +VK+AD  +C ++K++GR+ SK +  HSYPFC+RS TPLIY+A+P WFV VE IR+R++A+N  +YWVPSFV+EKRF NWL   KDW +SR RFWGTP+P+W+SDD EEVV VGSIAEL+EL+G +VTDLHREF+DH+TIPSK+GKG L+RV EVFDCWFESGSMPYAQ HYPFEN++KFE NFPADFVAEGLDQTRGWFYTL VLSTALFDKPAFKNLIVNGLVLA DGRKMSK LKN+ DP +++  +GADALRLYLINSPVVRAEPLKF   GV   +R++ LPWFN+ RFF QQA RL++  G  FVP+ +AA +S NVMD+WI+ A+  L+KFV+ EMKAYRLYTVVPRLV FI +LTNWYVRLNR RLKG  G+  A   L  +YEV   ++ LMAPFTPFF+EY Y  LR+ H NV N   +++AEDA G + SVH LM+P  D SRVD   E  M ++Q+VI +GR  R++R+ISLK PVK + VV  +Q+ L+ L +L+ Y+  ELN  +++   DEK WC L AEAN++ LG+RLGK +  VKK++A++    +   +   S+ I G  L G+DL++KR F GD +++EA VS +G LMV++DTR+DE+L  Q  +RE+++R+QKLRKKAGL V + + V+FE+    G    A+     +++  +   P P+S  P HSVP+      V+EE+   D +  L   RP +      +  K    +V V   T  + S+ +  V+   +                 K   E FLDA+   + SG  +L W+
Sbjct:  333 VHQAPTFGEDDYRVCVREGVADKFT-LPDPLDDNGVFTDAVPLVKGLHVKKADDVICQDLKNRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIVANNKLTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELEELAGEKVTDLHREFIDHLTIPSKQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKDKFEANFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKNFTDPEEILQKYGADALRLYLINSPVVRAEPLKFQAPGVLGVIREIFLPWFNSARFFAQQATRLQLETGVAFVPNREAALASTNVMDSWIIAAMHNLIKFVHEEMKAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEAAVALSALYEVEYNLAKLMAPFTPFFTEYMYQFLRQFHPNVVNGAGKDLAEDADGVSPSVHFLMLPDFDASRVDEEVEELMKNLQSVIEMGRVVRERRTISLKNPVKKVIVVSNNQKALDGLRRLETYLHDELNMRDLEFSTDEKEWCVLKAEANSRALGRRLGKALSGVKKQIAQMTHDDVAAYVSSGSVTIDGHELTGEDLVVKREFKGDSKIFEADVSPEGNLMVIIDTREDEELKMQGCAREVITRVQKLRKKAGLVVQDKIHVYFEEKGGKGPISTAIQTFLPMISSTLGTAPAPLSLQPQHSVPI------VSEEAQFADSSVKLVVARPAVLFASADV-LKKHEETVPVEQFTAYVASMKYADVKAALESXXXXXXXXXXXXXXXLKANVEVFLDAKTFAKASGKPELAWL 1171          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A484DRB2_BRELC (Isoleucine--tRNA ligase n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484DRB2_BRELC)

HSP 1 Score: 891 bits (2303), Expect = 1.700e-304
Identity = 455/846 (53.78%), Postives = 598/846 (70.69%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENE---NVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFE--DNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVR---RESKPSLSLTVGEATVTVDLGKEYFLDARAAVRQSGSRDLEW 2945
            V Q+P FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G +VK+AD  LC ++KS+GR+ SK +  HSYPFC+RS TPLIY+A+P WFV VE IR+R++A+N Q+YWVPSFV+EKRF NWL   KDW +SR RFWGTP+P+W+SDD EEVV VGSIAEL++L+G +VTDLHREF+DH+TIPS +GKG L+RV EVFDCWFESGSMPYAQ HYPFEN+EKFE NFPADFVAEGLDQTRGWFYTL VLSTALFDKPAFKNLIVNGLVLA DGRKMSK LKN+ DP +++  +GADALRLYLINSPVVRAEP++F   GV   +R++ LPWFN+ RFF QQA RL++  G  FVP+ +AA +S NVMD+WI+ AL  L+KFV+ EM+AYRLYTVVPRLV FI +LTNWYVRLNR RLKG  G+  A   L  +YEV   ++ LM+PFTPFF+EY Y  LR+ H N   E   ++AEDA G + SVH L++P  D +RVD   E  M +MQ+VI +GR  R++R ISLK PVK + VV  +++TL+ L +L  Y+  ELN  +++L ++EK WC L AEAN++VLG+RLGK +  VK  +  +    +   +   S+ + G  L+GDDLM+KR F GD  ++EA VS +G +MV++D R+D++L  Q  +RE+++R+QKLRKKAGL V ++V V+FE  D T G +   A+     ++A  +   P P++  P HSV +  E+      S +      + RP +      + AK  S  V V+  T  + S+ +  V+     +  S+++    A+VT+   ++ FLDA+A  R SG+ DL W
Sbjct:  333 VHQSPTFGEDDYRVCVREGVADKFT-LPDPLDDNGIFTDVVPLVQGLHVKKADDVLCQDLKSRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIIANNKQTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELEKLTGEKVTDLHREFIDHLTIPSTQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKEKFEANFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKNFTDPEEILQKYGADALRLYLINSPVVRAEPVRFHAPGVLGVIREIFLPWFNSARFFAQQATRLQLETGVAFVPNREAALASTNVMDSWIIAALHNLIKFVHKEMQAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEAAVALSALYEVEYNLAKLMSPFTPFFTEYMYQFLRQFHPNQVLEASVDLAEDADGVSRSVHFLLLPDFDATRVDEEVEELMKNMQSVIEMGRVIRERRMISLKNPVKKVIVVSNNKKTLDGLRRLASYLHDELNMRDLELSSEEKQWCVLKAEANSRVLGRRLGKALASVKTHIKAMTHDDVAAFVARGSVTVDGFDLSGDDLMVKREFQGDATIFEADVSPEGNVMVIIDVREDDELKMQGCAREVITRVQKLRKKAGLVVQDMVHVYFEEKDGTNGPIT-VAIQTFLPMIASTLGTSPAPLALQPPHSVSIVSETAQFAGSSVK----LIVARPAVHFALPNVLAKHESL-VPVSQFTAYVASMKYQDVKTALESADASVTVRTATASVTLKANEDVFLDAKAFARSSGNPDLAW 1171          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A7S3XRA7_HETAK (Isoleucine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XRA7_HETAK)

HSP 1 Score: 883 bits (2281), Expect = 2.260e-302
Identity = 449/796 (56.41%), Postives = 552/796 (69.35%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSC----GERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNVENENVAEDAFGRASSVHMLMIPPVDESRV-DPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFFEDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTFLTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLD 2804
            V QAPAFGEDDYRVCL HG+I K E+ PCPVD NG FT  V  + G+++KEAD  +C  +K +GR+  K  Y HSYPFCWRSDTPLIYKAVPSWFV+VE I+ERLLA+N Q+YWVP+FVKEKRF NWL  AKDWA+SRNRFWGTP+P+W SDD +E+VAVGS+ EL+ LSGV V DLHRE +DHITIPSK+G+G L+RVDEVFDCWFESGSMPYAQ HYPFEN+E+FE  FPADF+AEGLDQTRGWFYTL V+STALFDKPAFKNLIVNGLVLA DG+KMSKRLKNYPDP  VV  +GADALRLYL+NSPVVRAEPL+F EEGV A V+DV LPW+NAFRFF+Q ARR E++     G RF PD  AAA SAN  D WIL A + L+KFV  EM AYRLYTVVP L+ F+  LTNWY+RLNR+RLKG +G   A   L C+Y+VL  + +LMAP TPFF+E  Y  LR       +   AEDA G+A+SVH   +P        D    A MA ++  + LGR AR++R++SLK PV+ + VVC  +  L+ L +L  YV+ ELNA  ++L ADE AWC  TAE NNK LGKRLGK +  V+   A+L    L       ++ + G  L  +DL+++R F GD   YEA  + DG  +V LDT +D  L     +RE+V+R+QKLRK AGLQ+ + VEVFFE+         A+A NA L+  A+   PLP+      ++ + RE   V            + RPC  ++   + A+        A V  L+ SLD
Sbjct:  263 VHQAPAFGEDDYRVCLEHGLIAKDEEAPCPVDFNGRFTEAVADFQGRHIKEADADICAHLKERGRLVQKAVYDHSYPFCWRSDTPLIYKAVPSWFVSVEKIKERLLANNDQTYWVPAFVKEKRFHNWLRDAKDWAISRNRFWGTPIPLWCSDDYQEIVAVGSVDELEALSGVRVEDLHREHIDHITIPSKEGRGALRRVDEVFDCWFESGSMPYAQQHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAGDGKKMSKRLKNYPDPSIVVSKYGADALRLYLVNSPVVRAEPLRFKEEGVLAVVKDVFLPWYNAFRFFMQNARRWELTHPEGGGRRFAPDAAAAAGSANPTDAWILAAKEDLIKFVRQEMGAYRLYTVVPELLTFLNHLTNWYIRLNRDRLKGKDGPAEAHAALCCLYDVLFALCLLMAPLTPFFAETLYQHLRPFRPEAADAGAAEDAPGKAASVHYCTLPAARAGAPSDAAIGAKMAVLRRAVELGRVARERRNLSLKHPVRAVVVVCADRAKLDGLRELAGYVRSELNAVTLELTADEDAWCKYTAETNNKALGKRLGKDLRAVRAAAARLTNDQLRAFQAEGALTLEGHALGAEDLVVRREFIGDTARYEADTAPDGSFVVALDTTRDAALEQMGTAREVVNRVQKLRKAAGLQMEDAVEVFFEEEAGKTAVAAALAANAGLLEGALGAAPLPLGARAPRALEIAREEAEVAGSR----CVVAVCRPCPVVDAARVGAQA-------AGVETLLASLD 1047          

BLAST of mRNA_H-paniculata_contig10837.535.1 vs. uniprot
Match: A0A0P1ALS0_PLAHL (Isoleucine--tRNA ligase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1ALS0_PLAHL)

HSP 1 Score: 882 bits (2278), Expect = 6.400e-301
Identity = 453/838 (54.06%), Postives = 586/838 (69.93%), Query Frame = 3

Query:  432 VMQAPAFGEDDYRVCLAHGVIDKGEDIPCPVDSNGLFTVEVGAYAGKYVKEADKALCDEIKSKGRMFSKDSYKHSYPFCWRSDTPLIYKAVPSWFVAVESIRERLLASNAQSYWVPSFVKEKRFQNWLEGAKDWAVSRNRFWGTPMPIWMSDDGEEVVAVGSIAELKELSGVEVTDLHREFVDHITIPSKKGKGDLKRVDEVFDCWFESGSMPYAQLHYPFENREKFEQNFPADFVAEGLDQTRGWFYTLTVLSTALFDKPAFKNLIVNGLVLASDGRKMSKRLKNYPDPMKVVDAHGADALRLYLINSPVVRAEPLKFLEEGVQATVRDVLLPWFNAFRFFVQQARRLEMSCGERFVPDPDAAASSANVMDTWILVALQGLVKFVNTEMKAYRLYTVVPRLVGFIEELTNWYVRLNRNRLKGGEGTESARTGLFCMYEVLTTMSVLMAPFTPFFSEYTYLSLRKCHSNV---ENENVAEDAFGRASSVHMLMIPPVDESRVDPRTEADMASMQAVINLGRFARDKRSISLKTPVKGITVVCKSQETLNALEKLQLYVKGELNAWEVKLEADEKAWCTLTAEANNKVLGKRLGKKIGDVKKELAKLDTAALWPLLEGESIEICGETLAGDDLMLKRGFNGDVEVYEAAVSEDGKLMVVLDTRQDEKLLAQKMSREIVSRIQKLRKKAGLQVGEVVEVFF-EDNTKGGLAGKAVAENASLVADAVRCLPLPVSYMPSHSVPVGRESFSVTEESDQEDFTTF--LTRPCLSMNEVALDAKCRSASVDVAVVTQLMTSLDFDRVRRESKP---SLSLTVGEATVTVDLGKEYFLDARAAVR 2918
            V QAP FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G YVK+AD  +C ++K++GR+ SK +  HSYPFC+RS TPLIY+A+P WFV VE IR+R++A+N  +YWVPSFV+EKRF NWL   KDW +SR RFWGTP+P+W+SDD EEVV VGSIAEL+ L+G +VTDLHREF+DH+TIPSKKGKG L RV EVFDCWFESGSMPYAQ HYPFEN+ KFE+ FPADFVAEGLDQTRGWFYTL VLSTALFDKPAF+NLIVNGLVLA DGRKMSK LKN+ DP +++  +GADALRLYLINSPVVRAEPLKF   GV   +R++ LPWFN+ RFF QQA RL++  G  FVPD ++A +S NVMD+WIL AL  L+KFV+ EMKAYRLYTVVPRLV FIE+LTNWYVRLNR RLKG  G+  A   L  +YEV   ++ LMAPFTPFF+EY Y  LR+ H NV     ++ AED  G + S+H LM+P  D  RVD   E  M ++Q VI +GR  R++R ISLK PVK + VV   QETL+ L +LQ Y+  ELN  +++   DEK WC L AE N++VLG+RLGK +  VK ++A++    +   L    + I G  L G+DL++KR F GD  ++EA VS +G LMV++DTR+DE+L  Q  +RE+++R+QKLRKKAGL V + + VF+ E  ++ G    A+    +++A A+   P+P+S  P +SV +      V+EE+   D +    + RP +      + AK  S  + +  +   ++S+ F  V+   +    SL++   +   T+    + FLDA A  +
Sbjct:  333 VHQAPTFGEDDYRVCVREGVTDKFT-LPDPLDDNGIFTEIVPLVQGLYVKKADDVICQDLKARGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIVANNKLTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELETLTGEKVTDLHREFIDHLTIPSKKGKGILHRVPEVFDCWFESGSMPYAQQHYPFENQLKFEKTFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFQNLIVNGLVLAEDGRKMSKSLKNFTDPEEILQQYGADALRLYLINSPVVRAEPLKFQPLGVFGVIREIFLPWFNSARFFAQQATRLQLETGISFVPDRESALASTNVMDSWILAALHNLIKFVHEEMKAYRLYTVVPRLVNFIEQLTNWYVRLNRPRLKGSAGSTEAAIALSTLYEVEYNLAKLMAPFTPFFTEYMYQFLRQFHPNVVTSSGQDFAEDVDGVSLSIHFLMLPDFDARRVDNDVEVLMKNLQCVIEMGRIVRERRMISLKNPVKKVIVVSNQQETLDGLSRLQTYLHDELNMRDLEFSTDEKKWCVLKAETNSRVLGRRLGKALASVKTQVAQMTHDDVAAYLSSRCVTIDGHVLKGEDLIVKRDFQGDSTIFEANVSSEGNLMVIIDTREDEELKMQGCAREVITRVQKLRKKAGLVVQDKIHVFYQEQGSEKGPITTAIQTFQTMIASALGTTPVPLSLQPPYSVTI------VSEEAQFADSSIMFVVARPAVFFASTDVLAKHESI-ISIEELKAYVSSMRFQDVQAALESIDGSLTVRTVKGETTLQGNVDVFLDAMALAK 1162